Lsi04G010850 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi04G010850
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Ring finger protein, putative
Location	chr04 : 13084864 .. 13085654 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAAGGTATTTAGAATAATATCTGTACGACTTCCATAACTATCTTTATCGACAAAAAATAAATATCTAAATATCTAAACTTAATTATTTACTTTTTTTCAAAAAAAAAAAAAAAAAAACTTAATTATTTACTCAGAAATAAAAAGAGAAAATATCTGACACAGTTTCCAAAAACCCCAAAACCTTTCCCCTTTACTCTTTCCTCCGCCGCCGCAATTCTCCGACGACCGTTCGGTGCCGCCGAGATTCTCTGGCCGCCGTCCGTTTTATGAGTGGCAGGTTGAAATGGCTACATCCCAAGTCCCTCCGTCTGTTACCTGGCACTTCACAGAGCTCGACGATCGGCTCTTCCAAATCCGCGGCCGGACTTTCTTCTTCGTCGCCGTTTTATTCGCCGTCATCCTTCTTGTAACTTTTATCTTCCTTTACGCCCGATGGGTCTGCCGATTCCATCAACTCACCACCTTCTCCGCCGCTGTCCCCGGCGGCCGGTCGCCGAGGTCGCCGCCGCAGCTGGGGCTCGCCGCAACGGCGATTAACTGCCTTCCGATCACTCTATACAAGACACCAACGGCGGAGAAGGATCCACGCGCTGTGGCCGCCGCTGCAGGGGAGTGCCCCATTTGCTTGGGCGTGTTTGAGGATGGGGAGAAGGTGAAGATTCTGCCGCCGTGCCGCCATTGTTACCATTCCGAGTGCGTTGACCGGTGGCTCCGATCGCAGTCGAGCTGCCCGCTTTGCCGTGTCTCTCCCTGTATCGATACCTCGAACAATCTTCAAATTGTTTGA

mRNA sequence

ATGCCAAGTTTCCAAAAACCCCAAAACCTTTCCCCTTTACTCTTTCCTCCGCCGCCGCAATTCTCCGACGACCGTTCGGTGCCGCCGAGATTCTCTGGCCGCCGTCCGTTTTATGAGTGGCAGGTTGAAATGGCTACATCCCAAGTCCCTCCGTCTGTTACCTGGCACTTCACAGAGCTCGACGATCGGCTCTTCCAAATCCGCGGCCGGACTTTCTTCTTCGTCGCCGTTTTATTCGCCGTCATCCTTCTTGTAACTTTTATCTTCCTTTACGCCCGATGGGTCTGCCGATTCCATCAACTCACCACCTTCTCCGCCGCTGTCCCCGGCGGCCGGTCGCCGAGGTCGCCGCCGCAGCTGGGGCTCGCCGCAACGGCGATTAACTGCCTTCCGATCACTCTATACAAGACACCAACGGCGGAGAAGGATCCACGCGCTGTGGCCGCCGCTGCAGGGGAGTGCCCCATTTGCTTGGGCGTGTTTGAGGATGGGGAGAAGGTGAAGATTCTGCCGCCGTGCCGCCATTGTTACCATTCCGAGTGCGTTGACCGGTGGCTCCGATCGCAGTCGAGCTGCCCGCTTTGCCGTGTCTCTCCCTGTATCGATACCTCGAACAATCTTCAAATTGTTTGA

Coding sequence (CDS)

ATGCCAAGTTTCCAAAAACCCCAAAACCTTTCCCCTTTACTCTTTCCTCCGCCGCCGCAATTCTCCGACGACCGTTCGGTGCCGCCGAGATTCTCTGGCCGCCGTCCGTTTTATGAGTGGCAGGTTGAAATGGCTACATCCCAAGTCCCTCCGTCTGTTACCTGGCACTTCACAGAGCTCGACGATCGGCTCTTCCAAATCCGCGGCCGGACTTTCTTCTTCGTCGCCGTTTTATTCGCCGTCATCCTTCTTGTAACTTTTATCTTCCTTTACGCCCGATGGGTCTGCCGATTCCATCAACTCACCACCTTCTCCGCCGCTGTCCCCGGCGGCCGGTCGCCGAGGTCGCCGCCGCAGCTGGGGCTCGCCGCAACGGCGATTAACTGCCTTCCGATCACTCTATACAAGACACCAACGGCGGAGAAGGATCCACGCGCTGTGGCCGCCGCTGCAGGGGAGTGCCCCATTTGCTTGGGCGTGTTTGAGGATGGGGAGAAGGTGAAGATTCTGCCGCCGTGCCGCCATTGTTACCATTCCGAGTGCGTTGACCGGTGGCTCCGATCGCAGTCGAGCTGCCCGCTTTGCCGTGTCTCTCCCTGTATCGATACCTCGAACAATCTTCAAATTGTTTGA

Protein sequence

MPSFQKPQNLSPLLFPPPPQFSDDRSVPPRFSGRRPFYEWQVEMATSQVPPSVTWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTTFSAAVPGGRSPRSPPQLGLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDGEKVKILPPCRHCYHSECVDRWLRSQSSCPLCRVSPCIDTSNNLQIV

Homology

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BLAST of Lsi04G010850 vs. Swiss-Prot
Match: ATL66_ARATH (RING-H2 finger protein ATL66 OS=Arabidopsis thaliana GN=ATL66 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 6.0e-29
Identity = 70/151 (46.36%), Postives = 88/151 (58.28%), Query Frame = 1


Query: 55  WHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTTFSAAVPGGRSP 114
           WH  + DDR FQI GRT FF   LF+V+L    + LY    C         A+ P  R  
Sbjct: 16  WHENQYDDRNFQIHGRTLFFALALFSVVLFFALLTLYIHRNCLPRDSINLHASSPD-RLT 75

Query: 115 RSPPQLGLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDGEKVKILPPCR 174
           R     GL    I  LP+ L +   AE++         EC ICLG FE+GEK+K+LPPC 
Sbjct: 76  RCRSG-GLDPAEIRSLPVVLCRRERAEEEEEK------ECCICLGGFEEGEKMKVLPPCS 135

Query: 175 HCYHSECVDRWLRSQSSCPLCRVSPCIDTSN 206
           HCYH ECVDRWL+++SSCPLCRVS  +D+S+
Sbjct: 136 HCYHCECVDRWLKTESSCPLCRVSIRVDSSS 158

[Close]

BLAST of Lsi04G010850 vs. Swiss-Prot
Match: ATL74_ARATH (RING-H2 finger protein ATL74 OS=Arabidopsis thaliana GN=ATL74 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.2e-15
Identity = 37/65 (56.92%), Postives = 42/65 (64.62%), Query Frame = 1


Query: 134 LYKTPTAEKDPRAVAAAAGECPICLGVFEDGEKVKILPPCRHCYHSECVDRWLRSQSSCP 193
           L K P AE     V  AA EC ICLG F DGE+V++LPPC H +H  C+D WL S SSCP
Sbjct: 85  LKKFPVAEYGSGEVKIAATECAICLGEFADGERVRVLPPCNHSFHMSCIDTWLVSHSSCP 144

Query: 194 LCRVS 199
            CR S
Sbjct: 145 NCRHS 149

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BLAST of Lsi04G010850 vs. Swiss-Prot
Match: ATL15_ARATH (E3 ubiquitin-protein ligase ATL15 OS=Arabidopsis thaliana GN=ATL15 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-14
Identity = 51/141 (36.17%), Postives = 70/141 (49.65%), Query Frame = 1


Query: 65  FQIRGRTFFFVAVLFAVILLVTFIF------LYARWVCRFHQLTTFSAAVPGG-RSPRSP 124
           F   GRT F       +I+LV+  F      +Y R  C  H L   S   PG   + R  
Sbjct: 27  FDDEGRTSFSPTTAIIMIVLVSVFFALGCISVYMRR-CLQHALGMDSGGGPGNWLNVRQT 86

Query: 125 PQLGLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDGEKVKILPPCRHCY 184
            + GL A+ I   P   Y T    +    +   A ECP+CL  FED E ++++P C H +
Sbjct: 87  TEPGLDASVIETFPTFPYSTVKTLR----IGKEALECPVCLNEFEDDETLRLIPQCCHVF 146

Query: 185 HSECVDRWLRSQSSCPLCRVS 199
           H  C+D WLRSQ++CPLCR +
Sbjct: 147 HPGCIDAWLRSQTTCPLCRAN 162

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BLAST of Lsi04G010850 vs. Swiss-Prot
Match: ATL2_ARATH (RING-H2 finger protein ATL2 OS=Arabidopsis thaliana GN=ATL2 PE=2 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 3.2e-14
Identity = 46/135 (34.07%), Postives = 64/135 (47.41%), Query Frame = 1


Query: 75  VAVLFAVILLVTFIFLYARWVC----RFH---------QLTTFSAAVPGGRSPRSPPQLG 134
           + +LF V++L+ F+ LYARW      R H             F  A P   +       G
Sbjct: 35  IVILFFVVILMVFLHLYARWYLLRARRRHLRRRSRNRRATMVFFTADPSTAATSVVASRG 94

Query: 135 LAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDGEKVKILPPCRHCYHSEC 194
           L    I  LP+  +   T  KDP        EC +CL  FE+ E  ++LP C+H +H +C
Sbjct: 95  LDPNVIKSLPVFTFSDET-HKDPI-------ECAVCLSEFEESETGRVLPNCQHTFHVDC 154

Query: 195 VDRWLRSQSSCPLCR 197
           +D W  S S+CPLCR
Sbjct: 155 IDMWFHSHSTCPLCR 161

[Close]

BLAST of Lsi04G010850 vs. Swiss-Prot
Match: ATL72_ARATH (RING-H2 finger protein ATL72 OS=Arabidopsis thaliana GN=ATL72 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 4.2e-14
Identity = 37/89 (41.57%), Postives = 52/89 (58.43%), Query Frame = 1


Query: 121 GLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDGEKVKILPPCRHCYHSE 180
           GL   A+  +P+ LY +   +         A EC ICLG FEDGEKV++LP C H +H  
Sbjct: 88  GLKKQALKQIPVGLYGSGIIDMK-------ATECLICLGDFEDGEKVRVLPKCNHGFHVR 147

Query: 181 CVDRWLRSQSSCPLCRVSPCIDTSNNLQI 210
           C+D WL S+SSCP CR S  ++  + + +
Sbjct: 148 CIDTWLLSRSSCPTCRQSLLLEQPSPMAV 169

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BLAST of Lsi04G010850 vs. TrEMBL
Match: A0A0A0KLZ2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G210015 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 9.5e-74
Identity = 141/168 (83.93%), Postives = 146/168 (86.90%), Query Frame = 1


Query: 44  MATSQVPPSVTWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT 103
           MATSQVPPSV WHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT
Sbjct: 1   MATSQVPPSVPWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT 60

Query: 104 FSAAVPGGRSPRSPPQLGLAATAINCLPITLYKTPTAEKD-PRAVAAAAGECPICLGVFE 163
           FSA +P  R P SPPQ GL AT I  LPITLYK P A++D P A A  AGEC ICLGVFE
Sbjct: 61  FSAPLPVHRLPSSPPQQGLGATTIISLPITLYKPPAAKEDAPGAAANDAGECSICLGVFE 120

Query: 164 DGEKVKILPPCRHCYHSECVDRWLRSQSSCPLCRVSPCIDTSNNLQIV 211
           DGEKVKILPPCRHCYHSECVDRWLRS SSCPLCRVS CID SNNL++V
Sbjct: 121 DGEKVKILPPCRHCYHSECVDRWLRSHSSCPLCRVSLCIDPSNNLEMV 168

[Close]

BLAST of Lsi04G010850 vs. TrEMBL
Match: W9SF32_9ROSA (RING-H2 finger protein ATL66 OS=Morus notabilis GN=L484_000021 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.7e-38
Identity = 79/153 (51.63%), Postives = 100/153 (65.36%), Query Frame = 1


Query: 51  PSVTWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTTFSAAVPG 110
           P+  WH+T LDDR FQIRG+T FF+ V+F+ +L+VT + LYARW CR+  +     A   
Sbjct: 8   PTTQWHYTVLDDRSFQIRGKTLFFIVVIFSALLVVTLLLLYARWACRYRHVAASPYASQP 67

Query: 111 GRSPRSPPQLGLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDGEKVKIL 170
             +P +PP  GL A AI  LP+  +K+  +          A EC ICLGVFEDGEKVK+L
Sbjct: 68  QTAPPNPPPQGLDAVAIKGLPVISHKSAPSSD-------MAAECSICLGVFEDGEKVKVL 127

Query: 171 PPCRHCYHSECVDRWLRSQSSCPLCRVSPCIDT 204
           P C HCYH ECVDRWL +Q SCPLCR S  +D+
Sbjct: 128 PGCSHCYHPECVDRWLSAQPSCPLCRASLRVDS 153

[Close]

BLAST of Lsi04G010850 vs. TrEMBL
Match: A0A061FKY9_THECC (RING/U-box superfamily protein, putative OS=Theobroma cacao GN=TCM_042472 PE=4 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 8.4e-38
Identity = 82/156 (52.56%), Postives = 104/156 (66.67%), Query Frame = 1


Query: 44  MATSQVPPSVTWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT 103
           MA+SQ      WH+ EL+DR FQIRGRT FF+ VLF+ +L+ T IF+Y+RW+C FH+  +
Sbjct: 1   MASSQDSQPFHWHYAELEDRDFQIRGRTLFFIIVLFSFVLVFTLIFVYSRWICSFHRDNS 60

Query: 104 FSAAVPGGRSPRSPPQ-LGLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFE 163
            + +      P+  P+  GL    IN LPIT+  T   +    A  A   EC ICLGVFE
Sbjct: 61  STRSPTSHAPPQPRPEPRGLDPVTINALPITMV-TRGRKSAAAAAVALESECCICLGVFE 120

Query: 164 DGEKVKILPPCRHCYHSECVDRWLRSQSSCPLCRVS 199
           DGEKVK+LP C+H YHSECVDRWL ++SSCPLCR S
Sbjct: 121 DGEKVKVLPACQHSYHSECVDRWLSAESSCPLCRAS 155

[Close]

BLAST of Lsi04G010850 vs. TrEMBL
Match: A0A068V266_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00039150001 PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.4e-37
Identity = 81/158 (51.27%), Postives = 104/158 (65.82%), Query Frame = 1


Query: 46  TSQVPPSVTWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTTFS 105
           ++Q  P+  WH+++ DD  FQIRGRT FF  VLF VIL +T I+LYARWVCR    ++  
Sbjct: 95  SNQDSPTFHWHYSDFDDSNFQIRGRTLFFAVVLFCVILFITLIYLYARWVCRLPPFSSRP 154

Query: 106 AAVPGGRSPRSPPQLGLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDGE 165
             +P   S    PQ GL   AI+ LPI L++  +A  +        GEC ICLG+F+DGE
Sbjct: 155 RLLPDAPSGPPAPQ-GLDLNAISNLPIILHRASSANPNE-------GECAICLGIFQDGE 214

Query: 166 KVKILPPCRHCYHSECVDRWLRSQSSCPLCRVSPCIDT 204
           KVK+LP C H YHSEC+D WLR+QSSCPLCR S  +D+
Sbjct: 215 KVKVLPDCNHRYHSECIDEWLRTQSSCPLCRASLRVDS 244

[Close]

BLAST of Lsi04G010850 vs. TrEMBL
Match: A0A067FNF6_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031354mg PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.4e-37
Identity = 87/160 (54.37%), Postives = 106/160 (66.25%), Query Frame = 1


Query: 47  SQVPPSVTWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTTFSA 106
           SQ      WH+TELDD  FQIRGRT F + V+FAV+LLV+F+FLYARW CR      FS 
Sbjct: 3   SQESQPFHWHYTELDDHNFQIRGRTLFSIIVVFAVVLLVSFLFLYARWFCR-----RFSH 62

Query: 107 AVPGGRSPRSPPQL--GLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDG 166
                  PR+   L  GL  T IN LPI L+++  + KD +   +   EC ICLGVFEDG
Sbjct: 63  QNSNPDVPRAQQALTRGLDLTTINTLPIVLHRSEKSTKDDKEEES---ECCICLGVFEDG 122

Query: 167 EKVKILPPCRHCYHSECVDRWLRSQSSCPLCRVSPCIDTS 205
           +KVKILP CRHCYH ECVD WL ++SSCPLCR S  +D++
Sbjct: 123 DKVKILPKCRHCYHCECVDAWLSARSSCPLCRNSLRVDSA 154

[Close]

BLAST of Lsi04G010850 vs. TAIR10
Match: AT3G11110.1 (AT3G11110.1 RING/U-box superfamily protein)

HSP 1 Score: 129.0 bits (323), Expect = 3.4e-30
Identity = 70/151 (46.36%), Postives = 88/151 (58.28%), Query Frame = 1


Query: 55  WHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTTFSAAVPGGRSP 114
           WH  + DDR FQI GRT FF   LF+V+L    + LY    C         A+ P  R  
Sbjct: 16  WHENQYDDRNFQIHGRTLFFALALFSVVLFFALLTLYIHRNCLPRDSINLHASSPD-RLT 75

Query: 115 RSPPQLGLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDGEKVKILPPCR 174
           R     GL    I  LP+ L +   AE++         EC ICLG FE+GEK+K+LPPC 
Sbjct: 76  RCRSG-GLDPAEIRSLPVVLCRRERAEEEEEK------ECCICLGGFEEGEKMKVLPPCS 135

Query: 175 HCYHSECVDRWLRSQSSCPLCRVSPCIDTSN 206
           HCYH ECVDRWL+++SSCPLCRVS  +D+S+
Sbjct: 136 HCYHCECVDRWLKTESSCPLCRVSIRVDSSS 158

[Close]

BLAST of Lsi04G010850 vs. TAIR10
Match: AT5G01880.1 (AT5G01880.1 RING/U-box superfamily protein)

HSP 1 Score: 84.0 bits (206), Expect = 1.2e-16
Identity = 37/65 (56.92%), Postives = 42/65 (64.62%), Query Frame = 1


Query: 134 LYKTPTAEKDPRAVAAAAGECPICLGVFEDGEKVKILPPCRHCYHSECVDRWLRSQSSCP 193
           L K P AE     V  AA EC ICLG F DGE+V++LPPC H +H  C+D WL S SSCP
Sbjct: 85  LKKFPVAEYGSGEVKIAATECAICLGEFADGERVRVLPPCNHSFHMSCIDTWLVSHSSCP 144

Query: 194 LCRVS 199
            CR S
Sbjct: 145 NCRHS 149

[Close]

BLAST of Lsi04G010850 vs. TAIR10
Match: AT1G22500.1 (AT1G22500.1 RING/U-box superfamily protein)

HSP 1 Score: 80.9 bits (198), Expect = 1.0e-15
Identity = 51/141 (36.17%), Postives = 70/141 (49.65%), Query Frame = 1


Query: 65  FQIRGRTFFFVAVLFAVILLVTFIF------LYARWVCRFHQLTTFSAAVPGG-RSPRSP 124
           F   GRT F       +I+LV+  F      +Y R  C  H L   S   PG   + R  
Sbjct: 27  FDDEGRTSFSPTTAIIMIVLVSVFFALGCISVYMRR-CLQHALGMDSGGGPGNWLNVRQT 86

Query: 125 PQLGLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDGEKVKILPPCRHCY 184
            + GL A+ I   P   Y T    +    +   A ECP+CL  FED E ++++P C H +
Sbjct: 87  TEPGLDASVIETFPTFPYSTVKTLR----IGKEALECPVCLNEFEDDETLRLIPQCCHVF 146

Query: 185 HSECVDRWLRSQSSCPLCRVS 199
           H  C+D WLRSQ++CPLCR +
Sbjct: 147 HPGCIDAWLRSQTTCPLCRAN 162

[Close]

BLAST of Lsi04G010850 vs. TAIR10
Match: AT3G16720.1 (AT3G16720.1 TOXICOS EN LEVADURA 2)

HSP 1 Score: 80.1 bits (196), Expect = 1.8e-15
Identity = 46/135 (34.07%), Postives = 64/135 (47.41%), Query Frame = 1


Query: 75  VAVLFAVILLVTFIFLYARWVC----RFH---------QLTTFSAAVPGGRSPRSPPQLG 134
           + +LF V++L+ F+ LYARW      R H             F  A P   +       G
Sbjct: 35  IVILFFVVILMVFLHLYARWYLLRARRRHLRRRSRNRRATMVFFTADPSTAATSVVASRG 94

Query: 135 LAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDGEKVKILPPCRHCYHSEC 194
           L    I  LP+  +   T  KDP        EC +CL  FE+ E  ++LP C+H +H +C
Sbjct: 95  LDPNVIKSLPVFTFSDET-HKDPI-------ECAVCLSEFEESETGRVLPNCQHTFHVDC 154

Query: 195 VDRWLRSQSSCPLCR 197
           +D W  S S+CPLCR
Sbjct: 155 IDMWFHSHSTCPLCR 161

[Close]

BLAST of Lsi04G010850 vs. TAIR10
Match: AT3G10910.1 (AT3G10910.1 RING/U-box superfamily protein)

HSP 1 Score: 79.7 bits (195), Expect = 2.3e-15
Identity = 37/89 (41.57%), Postives = 52/89 (58.43%), Query Frame = 1


Query: 121 GLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDGEKVKILPPCRHCYHSE 180
           GL   A+  +P+ LY +   +         A EC ICLG FEDGEKV++LP C H +H  
Sbjct: 88  GLKKQALKQIPVGLYGSGIIDMK-------ATECLICLGDFEDGEKVRVLPKCNHGFHVR 147

Query: 181 CVDRWLRSQSSCPLCRVSPCIDTSNNLQI 210
           C+D WL S+SSCP CR S  ++  + + +
Sbjct: 148 CIDTWLLSRSSCPTCRQSLLLEQPSPMAV 169

[Close]

BLAST of Lsi04G010850 vs. NCBI nr
Match: gi|659109653|ref|XP_008454808.1| (PREDICTED: RING-H2 finger protein ATL66 [Cucumis melo])

HSP 1 Score: 296.6 bits (758), Expect = 3.5e-77
Identity = 145/168 (86.31%), Postives = 151/168 (89.88%), Query Frame = 1


Query: 44  MATSQVPPSVTWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT 103
           MATSQVPPSV WHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT
Sbjct: 1   MATSQVPPSVPWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT 60

Query: 104 FSAAVPGGRSPRSPPQL-GLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFE 163
           FSA++P  R PRSPPQ  GL ATAIN LPITLYK P AE+DP A A+ AGEC ICLGVFE
Sbjct: 61  FSASLPVRRQPRSPPQQQGLGATAINSLPITLYKAPAAEEDPPAAASDAGECSICLGVFE 120

Query: 164 DGEKVKILPPCRHCYHSECVDRWLRSQSSCPLCRVSPCIDTSNNLQIV 211
           DGEKVKILPPCRHCYHSECVDRWLRS SSCPLCRVS CID SNNL++V
Sbjct: 121 DGEKVKILPPCRHCYHSECVDRWLRSHSSCPLCRVSLCIDPSNNLEMV 168

[Close]

BLAST of Lsi04G010850 vs. NCBI nr
Match: gi|700195498|gb|KGN50675.1| (hypothetical protein Csa_5G210015 [Cucumis sativus])

HSP 1 Score: 284.6 bits (727), Expect = 1.4e-73
Identity = 141/168 (83.93%), Postives = 146/168 (86.90%), Query Frame = 1


Query: 44  MATSQVPPSVTWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT 103
           MATSQVPPSV WHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT
Sbjct: 1   MATSQVPPSVPWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT 60

Query: 104 FSAAVPGGRSPRSPPQLGLAATAINCLPITLYKTPTAEKD-PRAVAAAAGECPICLGVFE 163
           FSA +P  R P SPPQ GL AT I  LPITLYK P A++D P A A  AGEC ICLGVFE
Sbjct: 61  FSAPLPVHRLPSSPPQQGLGATTIISLPITLYKPPAAKEDAPGAAANDAGECSICLGVFE 120

Query: 164 DGEKVKILPPCRHCYHSECVDRWLRSQSSCPLCRVSPCIDTSNNLQIV 211
           DGEKVKILPPCRHCYHSECVDRWLRS SSCPLCRVS CID SNNL++V
Sbjct: 121 DGEKVKILPPCRHCYHSECVDRWLRSHSSCPLCRVSLCIDPSNNLEMV 168

[Close]

BLAST of Lsi04G010850 vs. NCBI nr
Match: gi|778708415|ref|XP_004148199.2| (PREDICTED: RING-H2 finger protein ATL66 [Cucumis sativus])

HSP 1 Score: 283.1 bits (723), Expect = 3.9e-73
Identity = 140/167 (83.83%), Postives = 145/167 (86.83%), Query Frame = 1


Query: 44  MATSQVPPSVTWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT 103
           MATSQVPPSV WHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT
Sbjct: 1   MATSQVPPSVPWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTT 60

Query: 104 FSAAVPGGRSPRSPPQLGLAATAINCLPITLYKTPTAEKD-PRAVAAAAGECPICLGVFE 163
           FSA +P  R P SPPQ GL AT I  LPITLYK P A++D P A A  AGEC ICLGVFE
Sbjct: 61  FSAPLPVHRLPSSPPQQGLGATTIISLPITLYKPPAAKEDAPGAAANDAGECSICLGVFE 120

Query: 164 DGEKVKILPPCRHCYHSECVDRWLRSQSSCPLCRVSPCIDTSNNLQI 210
           DGEKVKILPPCRHCYHSECVDRWLRS SSCPLCRVS CID SNNL++
Sbjct: 121 DGEKVKILPPCRHCYHSECVDRWLRSHSSCPLCRVSLCIDPSNNLEM 167

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BLAST of Lsi04G010850 vs. NCBI nr
Match: gi|698500514|ref|XP_009796011.1| (PREDICTED: RING-H2 finger protein ATL66-like [Nicotiana sylvestris])

HSP 1 Score: 172.9 bits (437), Expect = 5.8e-40
Identity = 86/159 (54.09%), Postives = 107/159 (67.30%), Query Frame = 1


Query: 46  TSQVPPSVTWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTTFS 105
           +SQ   S  WH+ E DD  FQIRGRT FFV VLFA+IL+ T +FLYARWVC+F   ++  
Sbjct: 21  SSQNSHSFHWHYAEFDDNDFQIRGRTLFFVVVLFAIILIATLLFLYARWVCKFRSPSSAE 80

Query: 106 AAVPGGRSPRSPPQLGLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFEDGE 165
           AA         PP  GL    I+ LPI L+++     D R   AA  EC ICLG+F+DG+
Sbjct: 81  AARSSHAPSVPPPPQGLDPAFIDNLPIILHRS----SDCRGNTAAEVECCICLGIFQDGD 140

Query: 166 KVKILPPCRHCYHSECVDRWLRSQSSCPLCRVSPCIDTS 205
           KVK+LP C+HCYHSECVD+WLR+QSSCPLCR S   D++
Sbjct: 141 KVKVLPSCQHCYHSECVDKWLRNQSSCPLCRASIQFDSA 175

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BLAST of Lsi04G010850 vs. NCBI nr
Match: gi|697113112|ref|XP_009610438.1| (PREDICTED: RING-H2 finger protein ATL66-like [Nicotiana tomentosiformis])

HSP 1 Score: 172.2 bits (435), Expect = 9.8e-40
Identity = 88/155 (56.77%), Postives = 106/155 (68.39%), Query Frame = 1


Query: 46  TSQVPPSVTWHFTELDDRLFQIRGRTFFFVAVLFAVILLVTFIFLYARWVCRFHQLTTFS 105
           +SQ   S  WH+ E DD  FQIRGRT FFV VLFA+IL+ T +FLYARWVC+F    +  
Sbjct: 21  SSQNSHSFHWHYAEFDDNDFQIRGRTLFFVVVLFAIILIATLLFLYARWVCKFRSPLSAE 80

Query: 106 AAVPGGRSPRS--PPQLGLAATAINCLPITLYKTPTAEKDPRAVAAAAGECPICLGVFED 165
           AA     +P S  PP  GL    I+ LPI L++T     D R   AA  EC ICLG+F+D
Sbjct: 81  AA-RSAHAPSSVPPPPQGLDPAFIDNLPIILHRT----SDCRGNTAAEVECCICLGIFQD 140

Query: 166 GEKVKILPPCRHCYHSECVDRWLRSQSSCPLCRVS 199
           G+KVK+LP C+HCYHSECVD+WLR+QSSCPLCR S
Sbjct: 141 GDKVKVLPSCQHCYHSECVDKWLRNQSSCPLCRAS 170

The following BLAST results are available for this feature:

BLAST of Lsi04G010850 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ATL66_ARATH	6.0e-29	46.36	RING-H2 finger protein ATL66 OS=Arabidopsis thaliana GN=ATL66 PE=2 SV=1	[more]
ATL74_ARATH	2.2e-15	56.92	RING-H2 finger protein ATL74 OS=Arabidopsis thaliana GN=ATL74 PE=2 SV=1	[more]
ATL15_ARATH	1.9e-14	36.17	E3 ubiquitin-protein ligase ATL15 OS=Arabidopsis thaliana GN=ATL15 PE=1 SV=1	[more]
ATL2_ARATH	3.2e-14	34.07	RING-H2 finger protein ATL2 OS=Arabidopsis thaliana GN=ATL2 PE=2 SV=2	[more]
ATL72_ARATH	4.2e-14	41.57	RING-H2 finger protein ATL72 OS=Arabidopsis thaliana GN=ATL72 PE=2 SV=1	[more]

BLAST of Lsi04G010850 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLZ2_CUCSA	9.5e-74	83.93	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G210015 PE=4 SV=1	[more]
W9SF32_9ROSA	1.7e-38	51.63	RING-H2 finger protein ATL66 OS=Morus notabilis GN=L484_000021 PE=4 SV=1	[more]
A0A061FKY9_THECC	8.4e-38	52.56	RING/U-box superfamily protein, putative OS=Theobroma cacao GN=TCM_042472 PE=4 S...	[more]
A0A068V266_COFCA	1.4e-37	51.27	Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00039150001 PE=4 SV=1	[more]
A0A067FNF6_CITSI	2.4e-37	54.38	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031354mg PE=4 SV=1	[more]

BLAST of Lsi04G010850 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G11110.1	3.4e-30	46.36	RING/U-box superfamily protein	[more]
AT5G01880.1	1.2e-16	56.92	RING/U-box superfamily protein	[more]
AT1G22500.1	1.0e-15	36.17	RING/U-box superfamily protein	[more]
AT3G16720.1	1.8e-15	34.07	TOXICOS EN LEVADURA 2	[more]
AT3G10910.1	2.3e-15	41.57	RING/U-box superfamily protein	[more]

BLAST of Lsi04G010850 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659109653\|ref\|XP_008454808.1\|	3.5e-77	86.31	PREDICTED: RING-H2 finger protein ATL66 [Cucumis melo]	[more]
gi\|700195498\|gb\|KGN50675.1\|	1.4e-73	83.93	hypothetical protein Csa_5G210015 [Cucumis sativus]	[more]
gi\|778708415\|ref\|XP_004148199.2\|	3.9e-73	83.83	PREDICTED: RING-H2 finger protein ATL66 [Cucumis sativus]	[more]
gi\|698500514\|ref\|XP_009796011.1\|	5.8e-40	54.09	PREDICTED: RING-H2 finger protein ATL66-like [Nicotiana sylvestris]	[more]
gi\|697113112\|ref\|XP_009610438.1\|	9.8e-40	56.77	PREDICTED: RING-H2 finger protein ATL66-like [Nicotiana tomentosiformis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008270	zinc ion binding
GO:0005515	protein binding

Vocabulary: **INTERPRO**
Term	Definition
IPR013083	Znf_RING/FYVE/PHD
IPR001841	Znf_RING

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044267	cellular protein metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G010850.1	Lsi04G010850.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 153..196 score: 2.9
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 154..195 score: 3.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 154..196 score: 12
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 152..197 score: 5.5
None	No IPR available	PANTHER	PTHR14155	RING FINGER DOMAIN-CONTAINING	coord: 61..198 score: 2.1
None	No IPR available	PANTHER	PTHR14155:SF100	RING-H2 FINGER PROTEIN ATL66	coord: 61..198 score: 2.1
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 130..202 score: 1.06

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi04G010850	CSPI05G10840	Wild cucumber (PI 183967)	cpilsiB373
Lsi04G010850	Cucsa.368380	Cucumber (Gy14) v1	cgylsiB597
Lsi04G010850	CmaCh04G001430	Cucurbita maxima (Rimu)	cmalsiB671
Lsi04G010850	CmoCh04G001390	Cucurbita moschata (Rifu)	cmolsiB663
Lsi04G010850	Cla004365	Watermelon (97103) v1	lsiwmB306
Lsi04G010850	MELO3C018366	Melon (DHL92) v3.5.1	lsimeB285
Lsi04G010850	ClCG07G004370	Watermelon (Charleston Gray)	lsiwcgB297
Lsi04G010850	MELO3C018366.2	Melon (DHL92) v3.6.1	lsimedB299
Lsi04G010850	Cla97C07G132830	Watermelon (97103) v2	lsiwmbB298
Lsi04G010850	Bhi07G000489	Wax gourd	lsiwgoB381
Lsi04G010850	CsGy5G008170	Cucumber (Gy14) v2	cgyblsiB338
Lsi04G010850	CsaV3_5G010310	Cucumber (Chinese Long) v3	cuclsiB394

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi04G010850	Cucurbita maxima (Rimu)	cmalsiB672
Lsi04G010850	Cucurbita moschata (Rifu)	cmolsiB662
Lsi04G010850	Cucurbita pepo (Zucchini)	cpelsiB354
Lsi04G010850	Cucurbita pepo (Zucchini)	cpelsiB355
Lsi04G010850	Cucumber (Chinese Long) v2	culsiB369
Lsi04G010850	Silver-seed gourd	carlsiB081
Lsi04G010850	Silver-seed gourd	carlsiB605