Lsi04G000340 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi04G000340
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionE3 ubiquitin-protein ligase
Locationchr04 : 495319 .. 498419 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACTCTAGTGTTGTTGAAATATCTAAGCTTCACCATAAGAATCTAGAACAGGCAATCAGGGGTTTGTCTTTTGTCCCATGAGTTTTTCCTATGCTATCTAAAATACTGAGGATCTGGAGCATTTGATTTTCGTGTTTTGTGGCTAAATTGTTCATTTTGTCTTCCCTGGTGTTTTTCTATGGTATTAAAATCCTAGGAGGCTTTGTAAGTACAGTACTTTTTTTATTTTGACAAACTAGAGAGCTTTCTCGTAATTTTTATTTTCCTTTTAAGAGGTCCAACTTACCAATCCCCTCTTATTTTTTAACGTCATTTCTTTATAGAGGAGTTTCTTATCCTGGGAAGGGTATCTTGATGAAGGAGCAATATTTCTCCTTGTTAACACTCTAAATACTTATTTTTCTTGGGTAGACCTATTGATTGACATATTATGTTCTCAATTCTTGCAAGTTCTTGTTTTTACTTTTTCTAACTCATGTTTATGCATGTAGGCATTCTATTGATAAAAATGTCATCTGCCAATCCTTATTTTGATGACATTCGGACCAAACCTGAGGTTATTGATCCTCCCCAAGATGATGACATGATGGATGTTAGTGAAAGTGTGAGTGATCCTGCTCGAACTGGAGGAAAACCTAATGTAGCTGTCTCTAGCAGTGTTCGTGAGCTGTTAGAGTGCCCTGTTTGCTTAAATGCTATGTACCCTCCCATTCATCAGGTCTGTTGTCATCTTTTTAAGATCTTTTGAATGAAATTGCTTTCTTGTTTAGAGAGAGGGCCTGGGTGAACTATGAAGTAGGTGGTGTCAGTGATGGACTGATGGAGTTGGTGGTGATGTTTGTTATGGAAAAGGTTTTGGTGGTGGGTTTGGAAGATTTAGGTTTTGTAGGTGGATTTGGTGGTGGGGCAGGCAGGGGATTCCGGTGGGGGAGGAAATGGACATCCCTAAACTTTGAAAAATCTCATTAATTATACCATATATCAATACAACTTTGTTGTTATTACCACATGAAACCATTCTCATGGTTACGGAAGAGATTACATAAATATCTGTCGAGAGAGTAACATAACAAAAAACAAAAGCAGAGTGGAAGATGAGAAAGCAACCCACTCTAATCCTCCAATTGACTTGAATTAATAGAATTGTATAATTACAACATAAATTGGATACTGATTCCAAATGAGTAATATAATCCTGAATCTCCTAAAAACTCCTGAACTCTCTTCAAAGAACCTATGAACGATTTTTTTTTTTTTTTTTTCATTCCACAGGTCACATTGAATTCCTCTAATCGTCCACACGATTTTGGCCTTCAACTTAAAGGTGTCTCTCGAAGGCACCAATGTAGGGCAAATATATTCAGCAGTTTAACCACAACTAGAAGCCATACCATATCTTAAGAGATAGATGATTTTGGCTTGTGTTCCTATACGAAATACACCGGTTTTGTTTGGATTAGTATAGCGGGTTAATGAAAAATCTTACTTTCTTAAGATACTTGTGCTTTGCATGGATGTCCTTGCCCAAATTCTTCATTTTGGCAATTAAAAAACAGAAGACAACCTAATCTGGCTATCACAAAACGAATTTGGAGGTAGAGAGCTGTTTTCCTCTTTAACCTAGACTAAGTGTTCTCCTTGAGTTTATGAATAATGTGAAAGGTCTGGATTTTTTTCCCTGTATTATTGAGAATACCATTTCTTTCCGGGATATATGACAATTATTTTTGTTTCTTGTATTCTTATGTCAATAAAATTCTGTTTTTGCAGTGTTCGAATGGTCACACGTTGTGTTCCGGTTGCAAGCCTAGGGTGCATAACCGATGTCCGACCTGCAGGCATGAACTTGGAAATATCAGATGTCTTGCATTAGAGAAGGTGGCTGCATCTCTGGAACTTCCATGTAAATATCAAACTTTTGGATGTATCGGCATATACCCATACTATAGCAAGCTGAAACACGAATCTCAGTGTGTGTATCGACCCTATAACTGTCCATATGCGGGATCGGAATGCACCGTTATCGGCGATATTCCATTTTTGGTAGCACACCTCAAGGATGATCACAAAGTTGACATGCATAATGGGAGCACTTTCAATCACCGGTATGTCAAGTCAAATCCGCAAGAGGTCGAAAATGCCACCTGGATGTTAACGGTACTATTCTTTTTTGTCTTATTTTGTCATGTTATGGTTTTTGGCCTGTTGAACTGTTGAACCTTGTTCAACATATTTTCAAATTAAGGTTTAATTTTTGATAGAAGAGGCTTTATGAGTTGAGAATAAACAGATGGACAGCGGACATGTCCTTTTTTTTATTAGATAGGGAGATCAGATAGCGAGATTGGATCGTATATGTTTCGAATTATGAGAGATCGGGCACGCAACTGATAACCTTTTTCTGTTTGATTTCTAGGTTTTCAGCTGTTTTGGTCAATACTTTTGTTTACATTTTGAAGCTTTCCAGCTTGGAATGGCTCCTGTCTACATAGCTTTCTTGCGGTTTATGGGCGATGATAATGAAGCAAAGAACTACAGTTATAGTCTTGAGGTTGGTGGAAATGGGAGGAAGATGGTGTGGCAAGGAGTGCCACGGAGTATTCGGGACAGTCATCGGAAGGTCCGAGACAGTTTTGACGGTCTTATTATCCAACGTAATATGGCGCTCTTCTTCTCAGGGGGAGATCGAAAAGAATTGAAACTCAGGGTAACTGGTAGGATTTGGAAGGAGCAGTAATAGTCTGTTTCTGATCACTTTTTCCTGTTCTATGTTTAGATCCTTAGAAAGTAAAAGTAGGCTAATTAGAGTAATGATATAATAGATCTCCAATCATGTAATGTGTAGTTATTTTATTTTTAGTTTTTAACATTCAAAGGCAAACTCTGTTTGCTTGTGGGAAATCAAATACATGAACTGGACTGCAAAAACCTGTGTTCTTACTTCACTGTTGCAAGAGAAAGGTGTTCTTTTATTTTTCTCTTTATTTTTTATTTTTACTTTCTGCCCCTCAGAAGACTTGAAATAACATTTGTAGGTCCAGTAGTTATTTCAATGGAAAAGCAACAGGAAAAGAGAGAGAGAGAGAGAGAGAGAGAGA

mRNA sequence

ATGCACTCTAGTGTTGTTGAAATATCTAAGCTTCACCATAAGAATCTAGAACAGGCAATCAGGGGCATTCTATTGATAAAAATGTCATCTGCCAATCCTTATTTTGATGACATTCGGACCAAACCTGAGGTTATTGATCCTCCCCAAGATGATGACATGATGGATGTTAGTGAAAGTGTGAGTGATCCTGCTCGAACTGGAGGAAAACCTAATGTAGCTGTCTCTAGCAGTGTTCGTGAGCTAGAGAGGGCCTGGGTGAACTATGAAGTAGGTGGTGTCAGTGATGGACTGATGGAGTTGGTGGTGATGTTTGTTATGGAAAAGTGTTCGAATGGTCACACGTTGTGTTCCGGTTGCAAGCCTAGGGTGCATAACCGATGTCCGACCTGCAGGCATGAACTTGGAAATATCAGATGTCTTGCATTAGAGAAGGTGGCTGCATCTCTGGAACTTCCATGTAAATATCAAACTTTTGGATGTATCGGCATATACCCATACTATAGCAAGCTGAAACACGAATCTCAGTGTGTGTATCGACCCTATAACTGTCCATATGCGGGATCGGAATGCACCGTTATCGGCGATATTCCATTTTTGGTAGCACACCTCAAGGATGATCACAAAGTTGACATGCATAATGGGAGCACTTTCAATCACCGGTATGTCAAGTCAAATCCGCAAGAGGTCGAAAATGCCACCTGGATGTTAACGGTTTTCAGCTGTTTTGGTCAATACTTTTGTTTACATTTTGAAGCTTTCCAGCTTGGAATGGCTCCTGTCTACATAGCTTTCTTGCGGTTTATGGGCGATGATAATGAAGCAAAGAACTACAGTTATAGTCTTGAGGTTGGTGGAAATGGGAGGAAGATGGTGTGGCAAGGAGTGCCACGGAGTATTCGGGACAGTCATCGGAAGGTCCGAGACAGTTTTGACGGTCTTATTATCCAACGTAATATGGCGCTCTTCTTCTCAGGGGGAGATCGAAAAGAATTGAAACTCAGGGTAACTGGTAGGATTTGGAAGGAGCAGTAATAGTCTGTTTCTGATCACTTTTTCCTGTTCTATGTTTAGATCCTTAGAAAGTAAAAGTAGGCTAATTAGAGTAATGATATAATAGATCTCCAATCATGTAATGTGTAGTTATTTTATTTTTAGTTTTTAACATTCAAAGGCAAACTCTGTTTGCTTGTGGGAAATCAAATACATGAACTGGACTGCAAAAACCTGTGTTCTTACTTCACTGTTGCAAGAGAAAGGTGTTCTTTTATTTTTCTCTTTATTTTTTATTTTTACTTTCTGCCCCTCAGAAGACTTGAAATAACATTTGTAGGTCCAGTAGTTATTTCAATGGAAAAGCAACAGGAAAAGAGAGAGAGAGAGAGAGAGAGAGAGA

Coding sequence (CDS)

ATGCACTCTAGTGTTGTTGAAATATCTAAGCTTCACCATAAGAATCTAGAACAGGCAATCAGGGGCATTCTATTGATAAAAATGTCATCTGCCAATCCTTATTTTGATGACATTCGGACCAAACCTGAGGTTATTGATCCTCCCCAAGATGATGACATGATGGATGTTAGTGAAAGTGTGAGTGATCCTGCTCGAACTGGAGGAAAACCTAATGTAGCTGTCTCTAGCAGTGTTCGTGAGCTAGAGAGGGCCTGGGTGAACTATGAAGTAGGTGGTGTCAGTGATGGACTGATGGAGTTGGTGGTGATGTTTGTTATGGAAAAGTGTTCGAATGGTCACACGTTGTGTTCCGGTTGCAAGCCTAGGGTGCATAACCGATGTCCGACCTGCAGGCATGAACTTGGAAATATCAGATGTCTTGCATTAGAGAAGGTGGCTGCATCTCTGGAACTTCCATGTAAATATCAAACTTTTGGATGTATCGGCATATACCCATACTATAGCAAGCTGAAACACGAATCTCAGTGTGTGTATCGACCCTATAACTGTCCATATGCGGGATCGGAATGCACCGTTATCGGCGATATTCCATTTTTGGTAGCACACCTCAAGGATGATCACAAAGTTGACATGCATAATGGGAGCACTTTCAATCACCGGTATGTCAAGTCAAATCCGCAAGAGGTCGAAAATGCCACCTGGATGTTAACGGTTTTCAGCTGTTTTGGTCAATACTTTTGTTTACATTTTGAAGCTTTCCAGCTTGGAATGGCTCCTGTCTACATAGCTTTCTTGCGGTTTATGGGCGATGATAATGAAGCAAAGAACTACAGTTATAGTCTTGAGGTTGGTGGAAATGGGAGGAAGATGGTGTGGCAAGGAGTGCCACGGAGTATTCGGGACAGTCATCGGAAGGTCCGAGACAGTTTTGACGGTCTTATTATCCAACGTAATATGGCGCTCTTCTTCTCAGGGGGAGATCGAAAAGAATTGAAACTCAGGGTAACTGGTAGGATTTGGAAGGAGCAGTAA

Protein sequence

MHSSVVEISKLHHKNLEQAIRGILLIKMSSANPYFDDIRTKPEVIDPPQDDDMMDVSESVSDPARTGGKPNVAVSSSVRELERAWVNYEVGGVSDGLMELVVMFVMEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCIGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ
Homology
[Close]
BLAST of Lsi04G000340 vs. Swiss-Prot
Match: SINA5_ARATH (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 446.4 bits (1147), Expect = 2.8e-124
Identity = 198/238 (83.19%), Postives = 218/238 (91.60%), Query Frame = 1 

Query: 106 MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCIGIYP 165
           + +C NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKVA SLELPCKY   GC+GI+P
Sbjct: 57  IHQCHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYNLGCLGIFP 116

Query: 166 YYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSN 225
           YYSKLKHESQC +RPY+CPYAGSEC  +GDI FLVAHL+DDHKVDMH G TFNHRYVKSN
Sbjct: 117 YYSKLKHESQCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHKVDMHTGCTFNHRYVKSN 176

Query: 226 PQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGG 285
           P+EVENATWMLTVF CFGQYFCLHFEAFQLGMAPVY+AFLRFMGD+++A+NY+YSLEVGG
Sbjct: 177 PREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLEVGG 236

Query: 286 NGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 344
           +GRK  W+G PRS+RDSHRKVRDS DGLIIQRNMALFFSGGD+KELKLRVTGRIWKEQ
Sbjct: 237 SGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDKKELKLRVTGRIWKEQ 294

[Close]
BLAST of Lsi04G000340 vs. Swiss-Prot
Match: SINA3_ARATH (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana GN=SINAT3 PE=2 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 4.0e-123
Identity = 198/238 (83.19%), Postives = 219/238 (92.02%), Query Frame = 1 

Query: 106 MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCIGIYP 165
           + +C NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKVA SLELPCK+ + GC  I+P
Sbjct: 74  IHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLELPCKHMSLGCPEIFP 133

Query: 166 YYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSN 225
           YYSKLKHE+ C +RPY+CPYAGSEC+V GDIPFLVAHL+DDHKVDMH+G TFNHRYVKSN
Sbjct: 134 YYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVDMHSGCTFNHRYVKSN 193

Query: 226 PQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGG 285
           P+EVENATWMLTVF CFGQYFCLHFEAFQLGMAPVY+AFLRFMGD+ EA+NY+YSLEVGG
Sbjct: 194 PREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGG 253

Query: 286 NGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 344
            GRK++W+G PRS+RDSHRKVRDS DGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ
Sbjct: 254 YGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 311

[Close]
BLAST of Lsi04G000340 vs. Swiss-Prot
Match: SINA4_ARATH (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana GN=SINAT4 PE=2 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 5.8e-122
Identity = 197/238 (82.77%), Postives = 217/238 (91.18%), Query Frame = 1 

Query: 106 MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCIGIYP 165
           + +C NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKVA SLELPCK+   GC  I+P
Sbjct: 75  IHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAESLELPCKFYNLGCPEIFP 134

Query: 166 YYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSN 225
           YYSKLKHES C +RPY+CPYAGSEC ++GDIPFLVAHL+DDHKVDMH GSTFNHRYVKSN
Sbjct: 135 YYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHKVDMHAGSTFNHRYVKSN 194

Query: 226 PQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGG 285
           P+EVENATWMLTVF CFGQYFCLHFEAFQLGM PVY+AFLRFMGD+ +A++YSYSLEVGG
Sbjct: 195 PREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFMGDEEDARSYSYSLEVGG 254

Query: 286 NGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 344
           +GRK+ W+G PRSIRDSHRKVRDS DGLIIQRNMALFFSGGDRKELKLRVTG+IWKEQ
Sbjct: 255 SGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDRKELKLRVTGKIWKEQ 312

[Close]
BLAST of Lsi04G000340 vs. Swiss-Prot
Match: SINA2_ARATH (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.3e-118
Identity = 191/238 (80.25%), Postives = 212/238 (89.08%), Query Frame = 1 

Query: 106 MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCIGIYP 165
           + +C NGHTLCS CK RV N CPTCR+ELGNIRCLALEKVA SLE+PC+YQ  GC  I+P
Sbjct: 71  IHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFP 130

Query: 166 YYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSN 225
           YYSKLKHE  C +RPY CPYAGSEC+V GDIP LV HLKDDHKVDMH+G TFNHRYVKSN
Sbjct: 131 YYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSN 190

Query: 226 PQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGG 285
           P EVENATWMLTVF+CFG+ FCLHFEAFQLGMAPVY+AFLRFMGD+NEAK +SYSLEVG 
Sbjct: 191 PHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGA 250

Query: 286 NGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 344
           +GRK+ WQG+PRSIRDSHRKVRDS DGLII RN+AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 251 HGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

[Close]
BLAST of Lsi04G000340 vs. Swiss-Prot
Match: SINA1_ARATH (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana GN=SINAT1 PE=3 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 1.4e-115
Identity = 187/238 (78.57%), Postives = 210/238 (88.24%), Query Frame = 1 

Query: 106 MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCIGIYP 165
           + +C NGHTLCS CK RV N CPTCR+ELGNIRCLALEKVA SLE+PC+YQ  GC  I+P
Sbjct: 68  IHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDIFP 127

Query: 166 YYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSN 225
           YYSKLKHE  C +R Y+CPYAGSEC+V GDIP LV HLKDDHKVDMH+G TFNHRYVKSN
Sbjct: 128 YYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVKSN 187

Query: 226 PQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGG 285
           P EVENATWMLTVF+CFG+ FCLHFEAFQLGMAPVY+AFLRFMGD+NEAK +SYSLEVG 
Sbjct: 188 PHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGA 247

Query: 286 NGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 344
           + RK+ WQG+PRSIRDSHRKVRDS DGLII RN+AL+FSG D++ELKLRVTGRIWKE+
Sbjct: 248 HSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWKEE 305

[Close]
BLAST of Lsi04G000340 vs. TrEMBL
Match: E5GCK0_CUCME (E3 ubiquitin-protein ligase OS=Cucumis melo subsp. melo PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 2.9e-165
Identity = 285/317 (89.91%), Postives = 292/317 (92.11%), Query Frame = 1 

Query: 28  MSSANPYFDDIRTKPEVIDPPQDDDMMDVSESVSDPARTGGKPNVAVSSSVRELERAWVN 87
           M+SANPYFDDIR KPEVIDPPQDDDMMDVSESVSDPAR GGKPNV VSSSVREL    V 
Sbjct: 1   MTSANPYFDDIRNKPEVIDPPQDDDMMDVSESVSDPARAGGKPNV-VSSSVRELLECPVC 60

Query: 88  YEVGGVSDGLMELVVMFV-MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 147
                       L  M+  + +CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA
Sbjct: 61  ------------LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 120

Query: 148 ASLELPCKYQTFGCIGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDD 207
           ASLELPCKYQTFGC+GIYPYYSKLKHESQC+YRPYNCPYAGSEC+VIGDIPFLVAHLKDD
Sbjct: 121 ASLELPCKYQTFGCVGIYPYYSKLKHESQCIYRPYNCPYAGSECSVIGDIPFLVAHLKDD 180

Query: 208 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 267
           HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR
Sbjct: 181 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 240

Query: 268 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 327
           FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG
Sbjct: 241 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 300

Query: 328 DRKELKLRVTGRIWKEQ 344
           DRKELKLRVTGRIWKEQ
Sbjct: 301 DRKELKLRVTGRIWKEQ 304

[Close]
BLAST of Lsi04G000340 vs. TrEMBL
Match: A0A0A0KRN7_CUCSA (E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_5G623730 PE=3 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 5.0e-165
Identity = 284/317 (89.59%), Postives = 293/317 (92.43%), Query Frame = 1 

Query: 28  MSSANPYFDDIRTKPEVIDPPQDDDMMDVSESVSDPARTGGKPNVAVSSSVRELERAWVN 87
           M+SANPYFDDIR KPEVIDPPQDDDMMDVSESVSDPAR+GGKPNV VSSSVREL    V 
Sbjct: 1   MTSANPYFDDIRNKPEVIDPPQDDDMMDVSESVSDPARSGGKPNV-VSSSVRELLECPVC 60

Query: 88  YEVGGVSDGLMELVVMFV-MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 147
                       L  M+  + +CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA
Sbjct: 61  ------------LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 120

Query: 148 ASLELPCKYQTFGCIGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDD 207
           ASLELPCKYQTFGC+GIYPYYSKLKHESQC+YRPYNCPYAGSEC+VIGDIPFLV+HLKDD
Sbjct: 121 ASLELPCKYQTFGCVGIYPYYSKLKHESQCIYRPYNCPYAGSECSVIGDIPFLVSHLKDD 180

Query: 208 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 267
           HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR
Sbjct: 181 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 240

Query: 268 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 327
           FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG
Sbjct: 241 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 300

Query: 328 DRKELKLRVTGRIWKEQ 344
           DRKELKLRVTGRIWKEQ
Sbjct: 301 DRKELKLRVTGRIWKEQ 304

[Close]
BLAST of Lsi04G000340 vs. TrEMBL
Match: A0A067KWI1_JATCU (E3 ubiquitin-protein ligase OS=Jatropha curcas GN=JCGZ_24516 PE=3 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 3.7e-160
Identity = 273/317 (86.12%), Postives = 289/317 (91.17%), Query Frame = 1 

Query: 28  MSSANPYFDDIRTKPEVIDPPQDDDMMDVSESVSDPARTGGKPNVAVSSSVRELERAWVN 87
           M+S NP+F+D+R+KPEVIDPPQ++DMMDVSESV+DPA+   KPNV VSSSVREL    V 
Sbjct: 1   MASGNPFFEDMRSKPEVIDPPQNEDMMDVSESVNDPAQMALKPNVTVSSSVRELLECPVC 60

Query: 88  YEVGGVSDGLMELVVMFV-MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 147
                       L  M+  + +CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA
Sbjct: 61  ------------LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 120

Query: 148 ASLELPCKYQTFGCIGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDD 207
           ASLELPCKYQ+FGC+GIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIP+LVAHLKDD
Sbjct: 121 ASLELPCKYQSFGCLGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPYLVAHLKDD 180

Query: 208 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 267
           HKVDMHNGSTFNHRYVKSNP EVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR
Sbjct: 181 HKVDMHNGSTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 240

Query: 268 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 327
           FMGDDNEAKNYSYSLEVGGNGRKM+WQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG
Sbjct: 241 FMGDDNEAKNYSYSLEVGGNGRKMIWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 300

Query: 328 DRKELKLRVTGRIWKEQ 344
           DRKELKLRVTGRIWKEQ
Sbjct: 301 DRKELKLRVTGRIWKEQ 305

[Close]
BLAST of Lsi04G000340 vs. TrEMBL
Match: B9RGC2_RICCO (E3 ubiquitin-protein ligase OS=Ricinus communis GN=RCOM_1452850 PE=3 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 1.8e-159
Identity = 271/317 (85.49%), Postives = 286/317 (90.22%), Query Frame = 1 

Query: 28  MSSANPYFDDIRTKPEVIDPPQDDDMMDVSESVSDPARTGGKPNVAVSSSVRELERAWVN 87
           M+S NP+FDD+R+KPEVIDPP  +DMMDV ESV+DPA+T  KPNV VSSSVREL    V 
Sbjct: 1   MASGNPFFDDLRSKPEVIDPPPSEDMMDVGESVNDPAQTAMKPNVTVSSSVRELLECPVC 60

Query: 88  YEVGGVSDGLMELVVMFV-MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 147
                       L  M+  + +CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA
Sbjct: 61  ------------LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 120

Query: 148 ASLELPCKYQTFGCIGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDD 207
           ASLELPCKY +FGC+GIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIP+LVAHLKDD
Sbjct: 121 ASLELPCKYHSFGCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPYLVAHLKDD 180

Query: 208 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 267
           HKVDMHNGSTFNHRYVKSNP EVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR
Sbjct: 181 HKVDMHNGSTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 240

Query: 268 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 327
           FMGDDNEAKNY+YSLEVGGNGRKM+WQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG
Sbjct: 241 FMGDDNEAKNYTYSLEVGGNGRKMIWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 300

Query: 328 DRKELKLRVTGRIWKEQ 344
           DRKELKLRVTGRIWKEQ
Sbjct: 301 DRKELKLRVTGRIWKEQ 305

[Close]
BLAST of Lsi04G000340 vs. TrEMBL
Match: A5CAJ5_VITVI (E3 ubiquitin-protein ligase OS=Vitis vinifera GN=VIT_13s0139g00150 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 7.7e-158
Identity = 271/317 (85.49%), Postives = 285/317 (89.91%), Query Frame = 1 

Query: 28  MSSANPYFDDIRTKPEVIDPPQDDDMMDVSESVSDPARTGGKPNVAVSSSVRELERAWVN 87
           M+S N YFDD+R+K EVIDPPQ +DMM+VSE V+DPA+T  KPNV VSSSVREL    V 
Sbjct: 1   MASGNTYFDDMRSKSEVIDPPQSEDMMEVSEHVNDPAQTTLKPNVTVSSSVRELLECPVC 60

Query: 88  YEVGGVSDGLMELVVMFV-MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 147
                       L  M+  + +CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA
Sbjct: 61  ------------LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 120

Query: 148 ASLELPCKYQTFGCIGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDD 207
           ASLELPCKYQ+FGC+GIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIP+LVAHLKDD
Sbjct: 121 ASLELPCKYQSFGCLGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPYLVAHLKDD 180

Query: 208 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 267
           HKVDMHNGSTFNHRYVKSNP EVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR
Sbjct: 181 HKVDMHNGSTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 240

Query: 268 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 327
           FMGDDNEAKNYSYSLEVGG GRKM+WQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG
Sbjct: 241 FMGDDNEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 300

Query: 328 DRKELKLRVTGRIWKEQ 344
           DRKELKLRVTGRIWKEQ
Sbjct: 301 DRKELKLRVTGRIWKEQ 305

[Close]
BLAST of Lsi04G000340 vs. TAIR10
Match: AT3G61790.1 (AT3G61790.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 442.6 bits (1137), Expect = 2.2e-124
Identity = 198/238 (83.19%), Postives = 219/238 (92.02%), Query Frame = 1 

Query: 106 MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCIGIYP 165
           + +C NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKVA SLELPCK+ + GC  I+P
Sbjct: 74  IHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLELPCKHMSLGCPEIFP 133

Query: 166 YYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSN 225
           YYSKLKHE+ C +RPY+CPYAGSEC+V GDIPFLVAHL+DDHKVDMH+G TFNHRYVKSN
Sbjct: 134 YYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVDMHSGCTFNHRYVKSN 193

Query: 226 PQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGG 285
           P+EVENATWMLTVF CFGQYFCLHFEAFQLGMAPVY+AFLRFMGD+ EA+NY+YSLEVGG
Sbjct: 194 PREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGG 253

Query: 286 NGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 344
            GRK++W+G PRS+RDSHRKVRDS DGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ
Sbjct: 254 YGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 311

[Close]
BLAST of Lsi04G000340 vs. TAIR10
Match: AT4G27880.1 (AT4G27880.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 438.7 bits (1127), Expect = 3.2e-123
Identity = 197/238 (82.77%), Postives = 217/238 (91.18%), Query Frame = 1 

Query: 106 MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCIGIYP 165
           + +C NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKVA SLELPCK+   GC  I+P
Sbjct: 75  IHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAESLELPCKFYNLGCPEIFP 134

Query: 166 YYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSN 225
           YYSKLKHES C +RPY+CPYAGSEC ++GDIPFLVAHL+DDHKVDMH GSTFNHRYVKSN
Sbjct: 135 YYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHKVDMHAGSTFNHRYVKSN 194

Query: 226 PQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGG 285
           P+EVENATWMLTVF CFGQYFCLHFEAFQLGM PVY+AFLRFMGD+ +A++YSYSLEVGG
Sbjct: 195 PREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFMGDEEDARSYSYSLEVGG 254

Query: 286 NGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 344
           +GRK+ W+G PRSIRDSHRKVRDS DGLIIQRNMALFFSGGDRKELKLRVTG+IWKEQ
Sbjct: 255 SGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDRKELKLRVTGKIWKEQ 312

[Close]
BLAST of Lsi04G000340 vs. TAIR10
Match: AT3G58040.1 (AT3G58040.1 seven in absentia of Arabidopsis 2)

HSP 1 Score: 426.8 bits (1096), Expect = 1.3e-119
Identity = 191/238 (80.25%), Postives = 212/238 (89.08%), Query Frame = 1 

Query: 106 MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCIGIYP 165
           + +C NGHTLCS CK RV N CPTCR+ELGNIRCLALEKVA SLE+PC+YQ  GC  I+P
Sbjct: 71  IHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFP 130

Query: 166 YYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSN 225
           YYSKLKHE  C +RPY CPYAGSEC+V GDIP LV HLKDDHKVDMH+G TFNHRYVKSN
Sbjct: 131 YYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSN 190

Query: 226 PQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGG 285
           P EVENATWMLTVF+CFG+ FCLHFEAFQLGMAPVY+AFLRFMGD+NEAK +SYSLEVG 
Sbjct: 191 PHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGA 250

Query: 286 NGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 344
           +GRK+ WQG+PRSIRDSHRKVRDS DGLII RN+AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 251 HGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

[Close]
BLAST of Lsi04G000340 vs. TAIR10
Match: AT2G41980.1 (AT2G41980.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 417.5 bits (1072), Expect = 7.7e-117
Identity = 187/238 (78.57%), Postives = 210/238 (88.24%), Query Frame = 1 

Query: 106 MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCIGIYP 165
           + +C NGHTLCS CK RV N CPTCR+ELGNIRCLALEKVA SLE+PC+YQ  GC  I+P
Sbjct: 68  IHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDIFP 127

Query: 166 YYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSN 225
           YYSKLKHE  C +R Y+CPYAGSEC+V GDIP LV HLKDDHKVDMH+G TFNHRYVKSN
Sbjct: 128 YYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVKSN 187

Query: 226 PQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGG 285
           P EVENATWMLTVF+CFG+ FCLHFEAFQLGMAPVY+AFLRFMGD+NEAK +SYSLEVG 
Sbjct: 188 PHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGA 247

Query: 286 NGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 344
           + RK+ WQG+PRSIRDSHRKVRDS DGLII RN+AL+FSG D++ELKLRVTGRIWKE+
Sbjct: 248 HSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWKEE 305

[Close]
BLAST of Lsi04G000340 vs. TAIR10
Match: AT5G53360.1 (AT5G53360.1 TRAF-like superfamily protein)

HSP 1 Score: 383.6 bits (984), Expect = 1.2e-106
Identity = 171/204 (83.82%), Postives = 189/204 (92.65%), Query Frame = 1 

Query: 140 LALEKVAASLELPCKYQTFGCIGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFL 199
           +ALEKVA SLELPCKY   GC+GI+PYYSKLKHESQC +RPY+CPYAGSEC  +GDI FL
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 200 VAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAP 259
           VAHL+DDHKVDMH G TFNHRYVKSNP+EVENATWMLTVF CFGQYFCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 260 VYIAFLRFMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNM 319
           VY+AFLRFMGD+++A+NY+YSLEVGG+GRK  W+G PRS+RDSHRKVRDS DGLIIQRNM
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 320 ALFFSGGDRKELKLRVTGRIWKEQ 344
           ALFFSGGD+KELKLRVTGRIWKEQ
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQ 218

[Close]
BLAST of Lsi04G000340 vs. NCBI nr
Match: gi|659092182|ref|XP_008446940.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT5-like [Cucumis melo])

HSP 1 Score: 589.3 bits (1518), Expect = 4.2e-165
Identity = 285/317 (89.91%), Postives = 292/317 (92.11%), Query Frame = 1 

Query: 28  MSSANPYFDDIRTKPEVIDPPQDDDMMDVSESVSDPARTGGKPNVAVSSSVRELERAWVN 87
           M+SANPYFDDIR KPEVIDPPQDDDMMDVSESVSDPAR GGKPNV VSSSVREL    V 
Sbjct: 1   MTSANPYFDDIRNKPEVIDPPQDDDMMDVSESVSDPARAGGKPNV-VSSSVRELLECPVC 60

Query: 88  YEVGGVSDGLMELVVMFV-MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 147
                       L  M+  + +CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA
Sbjct: 61  ------------LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 120

Query: 148 ASLELPCKYQTFGCIGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDD 207
           ASLELPCKYQTFGC+GIYPYYSKLKHESQC+YRPYNCPYAGSEC+VIGDIPFLVAHLKDD
Sbjct: 121 ASLELPCKYQTFGCVGIYPYYSKLKHESQCIYRPYNCPYAGSECSVIGDIPFLVAHLKDD 180

Query: 208 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 267
           HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR
Sbjct: 181 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 240

Query: 268 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 327
           FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG
Sbjct: 241 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 300

Query: 328 DRKELKLRVTGRIWKEQ 344
           DRKELKLRVTGRIWKEQ
Sbjct: 301 DRKELKLRVTGRIWKEQ 304

[Close]
BLAST of Lsi04G000340 vs. NCBI nr
Match: gi|778706762|ref|XP_011655908.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT5 [Cucumis sativus])

HSP 1 Score: 588.6 bits (1516), Expect = 7.2e-165
Identity = 284/317 (89.59%), Postives = 293/317 (92.43%), Query Frame = 1 

Query: 28  MSSANPYFDDIRTKPEVIDPPQDDDMMDVSESVSDPARTGGKPNVAVSSSVRELERAWVN 87
           M+SANPYFDDIR KPEVIDPPQDDDMMDVSESVSDPAR+GGKPNV VSSSVREL    V 
Sbjct: 1   MTSANPYFDDIRNKPEVIDPPQDDDMMDVSESVSDPARSGGKPNV-VSSSVRELLECPVC 60

Query: 88  YEVGGVSDGLMELVVMFV-MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 147
                       L  M+  + +CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA
Sbjct: 61  ------------LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 120

Query: 148 ASLELPCKYQTFGCIGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDD 207
           ASLELPCKYQTFGC+GIYPYYSKLKHESQC+YRPYNCPYAGSEC+VIGDIPFLV+HLKDD
Sbjct: 121 ASLELPCKYQTFGCVGIYPYYSKLKHESQCIYRPYNCPYAGSECSVIGDIPFLVSHLKDD 180

Query: 208 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 267
           HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR
Sbjct: 181 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 240

Query: 268 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 327
           FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG
Sbjct: 241 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 300

Query: 328 DRKELKLRVTGRIWKEQ 344
           DRKELKLRVTGRIWKEQ
Sbjct: 301 DRKELKLRVTGRIWKEQ 304

[Close]
BLAST of Lsi04G000340 vs. NCBI nr
Match: gi|802574062|ref|XP_012068638.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT5-like isoform X2 [Jatropha curcas])

HSP 1 Score: 572.4 bits (1474), Expect = 5.3e-160
Identity = 273/317 (86.12%), Postives = 289/317 (91.17%), Query Frame = 1 

Query: 28  MSSANPYFDDIRTKPEVIDPPQDDDMMDVSESVSDPARTGGKPNVAVSSSVRELERAWVN 87
           M+S NP+F+D+R+KPEVIDPPQ++DMMDVSESV+DPA+   KPNV VSSSVREL    V 
Sbjct: 1   MASGNPFFEDMRSKPEVIDPPQNEDMMDVSESVNDPAQMALKPNVTVSSSVRELLECPVC 60

Query: 88  YEVGGVSDGLMELVVMFV-MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 147
                       L  M+  + +CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA
Sbjct: 61  ------------LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 120

Query: 148 ASLELPCKYQTFGCIGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDD 207
           ASLELPCKYQ+FGC+GIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIP+LVAHLKDD
Sbjct: 121 ASLELPCKYQSFGCLGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPYLVAHLKDD 180

Query: 208 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 267
           HKVDMHNGSTFNHRYVKSNP EVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR
Sbjct: 181 HKVDMHNGSTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 240

Query: 268 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 327
           FMGDDNEAKNYSYSLEVGGNGRKM+WQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG
Sbjct: 241 FMGDDNEAKNYSYSLEVGGNGRKMIWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 300

Query: 328 DRKELKLRVTGRIWKEQ 344
           DRKELKLRVTGRIWKEQ
Sbjct: 301 DRKELKLRVTGRIWKEQ 305

[Close]
BLAST of Lsi04G000340 vs. NCBI nr
Match: gi|802574058|ref|XP_012068636.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT5-like isoform X1 [Jatropha curcas])

HSP 1 Score: 572.4 bits (1474), Expect = 5.3e-160
Identity = 273/317 (86.12%), Postives = 289/317 (91.17%), Query Frame = 1 

Query: 28  MSSANPYFDDIRTKPEVIDPPQDDDMMDVSESVSDPARTGGKPNVAVSSSVRELERAWVN 87
           M+S NP+F+D+R+KPEVIDPPQ++DMMDVSESV+DPA+   KPNV VSSSVREL    V 
Sbjct: 15  MASGNPFFEDMRSKPEVIDPPQNEDMMDVSESVNDPAQMALKPNVTVSSSVRELLECPVC 74

Query: 88  YEVGGVSDGLMELVVMFV-MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 147
                       L  M+  + +CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA
Sbjct: 75  ------------LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 134

Query: 148 ASLELPCKYQTFGCIGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDD 207
           ASLELPCKYQ+FGC+GIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIP+LVAHLKDD
Sbjct: 135 ASLELPCKYQSFGCLGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPYLVAHLKDD 194

Query: 208 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 267
           HKVDMHNGSTFNHRYVKSNP EVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR
Sbjct: 195 HKVDMHNGSTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 254

Query: 268 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 327
           FMGDDNEAKNYSYSLEVGGNGRKM+WQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG
Sbjct: 255 FMGDDNEAKNYSYSLEVGGNGRKMIWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 314

Query: 328 DRKELKLRVTGRIWKEQ 344
           DRKELKLRVTGRIWKEQ
Sbjct: 315 DRKELKLRVTGRIWKEQ 319

[Close]
BLAST of Lsi04G000340 vs. NCBI nr
Match: gi|1000979549|ref|XP_015570744.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT5 isoform X1 [Ricinus communis])

HSP 1 Score: 570.1 bits (1468), Expect = 2.6e-159
Identity = 271/317 (85.49%), Postives = 286/317 (90.22%), Query Frame = 1 

Query: 28  MSSANPYFDDIRTKPEVIDPPQDDDMMDVSESVSDPARTGGKPNVAVSSSVRELERAWVN 87
           M+S NP+FDD+R+KPEVIDPP  +DMMDV ESV+DPA+T  KPNV VSSSVREL    V 
Sbjct: 1   MASGNPFFDDLRSKPEVIDPPPSEDMMDVGESVNDPAQTAMKPNVTVSSSVRELLECPVC 60

Query: 88  YEVGGVSDGLMELVVMFV-MEKCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 147
                       L  M+  + +CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA
Sbjct: 61  ------------LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 120

Query: 148 ASLELPCKYQTFGCIGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPFLVAHLKDD 207
           ASLELPCKY +FGC+GIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIP+LVAHLKDD
Sbjct: 121 ASLELPCKYHSFGCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPYLVAHLKDD 180

Query: 208 HKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 267
           HKVDMHNGSTFNHRYVKSNP EVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR
Sbjct: 181 HKVDMHNGSTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 240

Query: 268 FMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 327
           FMGDDNEAKNY+YSLEVGGNGRKM+WQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG
Sbjct: 241 FMGDDNEAKNYTYSLEVGGNGRKMIWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGG 300

Query: 328 DRKELKLRVTGRIWKEQ 344
           DRKELKLRVTGRIWKEQ
Sbjct: 301 DRKELKLRVTGRIWKEQ 305

The following BLAST results are available for this feature:
BLAST of Lsi04G000340 vs. Swiss-Prot
Match NameE-valueIdentityDescription
SINA5_ARATH2.8e-12483.19E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana GN=SINAT5 PE=1 SV=2[more]
SINA3_ARATH4.0e-12383.19E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana GN=SINAT3 PE=2 SV=1[more]
SINA4_ARATH5.8e-12282.77E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana GN=SINAT4 PE=2 SV=1[more]
SINA2_ARATH2.3e-11880.25E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana GN=SINAT2 PE=1 SV=1[more]
SINA1_ARATH1.4e-11578.57Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana GN=SINAT1 PE... [more]
BLAST of Lsi04G000340 vs. TrEMBL
Match NameE-valueIdentityDescription
E5GCK0_CUCME2.9e-16589.91E3 ubiquitin-protein ligase OS=Cucumis melo subsp. melo PE=3 SV=1[more]
A0A0A0KRN7_CUCSA5.0e-16589.59E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_5G623730 PE=3 SV=1[more]
A0A067KWI1_JATCU3.7e-16086.12E3 ubiquitin-protein ligase OS=Jatropha curcas GN=JCGZ_24516 PE=3 SV=1[more]
B9RGC2_RICCO1.8e-15985.49E3 ubiquitin-protein ligase OS=Ricinus communis GN=RCOM_1452850 PE=3 SV=1[more]
A5CAJ5_VITVI7.7e-15885.49E3 ubiquitin-protein ligase OS=Vitis vinifera GN=VIT_13s0139g00150 PE=3 SV=1[more]
BLAST of Lsi04G000340 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G61790.12.2e-12483.19 Protein with RING/U-box and TRAF-like domains[more]
AT4G27880.13.2e-12382.77 Protein with RING/U-box and TRAF-like domains[more]
AT3G58040.11.3e-11980.25 seven in absentia of Arabidopsis 2[more]
AT2G41980.17.7e-11778.57 Protein with RING/U-box and TRAF-like domains[more]
AT5G53360.11.2e-10683.82 TRAF-like superfamily protein[more]
BLAST of Lsi04G000340 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659092182|ref|XP_008446940.1|4.2e-16589.91PREDICTED: E3 ubiquitin-protein ligase SINAT5-like [Cucumis melo][more]
gi|778706762|ref|XP_011655908.1|7.2e-16589.59PREDICTED: E3 ubiquitin-protein ligase SINAT5 [Cucumis sativus][more]
gi|802574062|ref|XP_012068638.1|5.3e-16086.12PREDICTED: E3 ubiquitin-protein ligase SINAT5-like isoform X2 [Jatropha curcas][more]
gi|802574058|ref|XP_012068636.1|5.3e-16086.12PREDICTED: E3 ubiquitin-protein ligase SINAT5-like isoform X1 [Jatropha curcas][more]
gi|1000979549|ref|XP_015570744.1|2.6e-15985.49PREDICTED: E3 ubiquitin-protein ligase SINAT5 isoform X1 [Ricinus communis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
GO:0007275multicellular organism development
GO:0006511ubiquitin-dependent protein catabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004842ubiquitin-protein transferase activity
GO:0008270zinc ion binding
GO:0005515protein binding
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
Vocabulary: INTERPRO
TermDefinition
IPR0181217-in-absentia-prot_TRAF-dom
IPR013323SIAH-type domain
IPR013083Znf_RING/FYVE/PHD
IPR013010Znf_SIAH
IPR008974TRAF-like
IPR004162SINA-like
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006915 apoptotic process
biological_process GO:0007275 multicellular organism development
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G000340.1Lsi04G000340.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004162E3 ubiquitin-protein ligase SIN-likePANTHERPTHR10315SEVEN IN ABSENTIA HOMOLOGcoord: 15..343
score: 1.9E
IPR008974TRAF-likeGENE3DG3DSA:2.60.210.10coord: 214..340
score: 1.1
IPR008974TRAF-likeunknownSSF49599TRAF domain-likecoord: 148..340
score: 5.1
IPR013010Zinc finger, SIAH-typePROFILEPS51081ZF_SIAHcoord: 148..208
score: 13
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 107..134
score: 2.
IPR013323SIAH-type domainGENE3DG3DSA:3.90.890.10coord: 148..203
score: 3.0
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 137..336
score: 2.5
NoneNo IPR availablePANTHERPTHR10315:SF29SUBFAMILY NOT NAMEDcoord: 15..343
score: 1.9E