Lsi03G015550 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G015550
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	tRNA-splicing endonuclease subunit Sen54
Location	chr03 : 26523179 .. 26527984 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGCTACGGATTGGGAAAGCTCTTCAGGAGGAGCCAGTGGTGATGACGACAATTACGAGCAAGACATAAAAGATGAAGAAGAATGTCTCTGCGCATCTGGGTGCTTGCGCAAGTTGCAATTCAGGTTGAAAATTTCAAGAATTTGAACTCCCCCGAGTCCTTCAATAGGATACTGTTATTTGCTTATTATTAAGTGTGTGTTTCAATATTTTATCTGTCTCTAGGAAGCATGCTTCAACTGCTCGATGGAACGATCAAATGGGAATGGCAGAAGTTTTAGAGAACAAGGGCAGCCTTTGGACGACAACTGGCATTGTACGTTGTGGCAAGATTTATTGTTCCATTGAGGAAACTTTGTAGGTTAAGTTATGTTTGTTTTTTGTTTTAATTTATCCCTATTGTTTTTTATATTGGCCACTTCTCTTTCACATTGTCTTAACTTAATTTGATTACTTTAAAGTAGTAGGGTCATATCATGACAAAGAGAAGAAATTGAAAGTGTTTCCAAGCCAAGGCCAGGAAATGGCCTATGAGAGCCGCATTAGGTGCATCTTGGCAATTACCTTAGTCGCCTCTTGGTCAGTTCTTTATTTGCTTTTGGTTGTCTTTGGGGCTGTATTTAGAACATTACATTTTAAATAAAGGGAAGGAATTCAAACTAAAATTTTACAAGAAGAAGCACTAGTAGCCTTTTGGTGTCTTAGTAAAGATGGGAAGTTATCATTTTTTAAGTTCTTTTTTAACTATAGTTTTTTTTTCCTAACATTATCTTTTTAAAATAAGTAAGAAATTTTATTAAGCTTAGTTGTGTCCAACTATATAATTCATTTTATGTATCACTTATTCTTCTTTTCTATTTACAATATATATACATCTACTAGTCTATTTCCAATAAATTTGCACATCTATTGTTTTACATTCACTTGAGCAATGTTCCTTTTTTTAATATTTTTACTGTGTCTCTTGTCTTAGGAAAGTTAGAAGCTTGTTGCCTTAAAGTGTATCTTGTACTTTGTAAATGTTGGTTGTAATATGAACTTGGCTCAAACGTAGAGTTGTACAAATTAAAGCAGAAAAATATTCTCTTTTTTGTTTTTTTTTTTGTTTTTTTTTTTGAACTCTATAAATAGAGATGTTAAGGTTCATAATCTCTTTTGAGACATTTGGATGACAATTTTTATGCACACTATCAAAACTTCTTTTCTATATTTTCAGATTTCTTATTGAAGTTGGGGCCTTAGATCTTTTGGATCATGATAATTCAAGTCTTTCTTTGAAAGATGTATATAAGAAGGTAGCTGAAGGAAAAAGTGGGTGTCTTTGGGAGCAGTTCGAGGTTTATAGGCACCTCAAATCTCTTGGTTTCATTGTTGGAAAGCATAAAGTTCCTTGGTCTCTGAAGAGTGTTAGGAATGACAGTGACGTTTCATCTCCAAGTTCTATTGAAAACAAAGGAGCATCTGATGTCAAATCAGAAGATGAGAGGTTGATCTCTGAACTATTAGATGGCATTCAGCTTGACGAAGTGACACCCATTTTTGATGTTTTCCTTCCGCATAACAAGTTTAAAAAGTCTTCTCCTGGTGACCCAAATTTTATGGTCTGCTTGACTAGGTAAGTTCACTTGTTATAATAAAATGACTTGCACCAGAATTGGAGAAGTGTGTTCTTTTATTCAATCACTTCTCACTATAAATACTAATTCTACGCTTAAAAATAGCTTACTTGATAACAAACTTTGGACTATTTCAGATACTAAAAGTAGACTTGCACTTGAGTTAAACTAACCTACTCCTCCTAACTCAAATGCTGCAAGCTCTCAAAAGCTTGAAATCGACTAACACTAAAAGGCTTAGTGAAGATATTTGCTATTTGCTCCTCTGAACGACAATGAACCAGTTGCAATTCTCCTTTCTTTTGCATATCTCTCAAAACATAATATTTTATCTTAAACTGTTTTGTTCTTCCATGAAAAATTGGATTCAAAGAAATAGTTAATGTGGCTTGATTATCCACAAACACCTTCACACTCTCTTCTTGCTTCAAATGTAAGTCATGTATCAACTTCTTCAACCAAACAACTTGGTTTATTGCTGCAACAAACTCATCTTCTGTAGTTGATTGAGCCATTATATTTTTTTTTTTTTTTGAACACCATGAGAAACACCAGAGCCAAAAGTGAGATAATACTTGGAGGTGCTCTTCATATCATCCAATGATCCACTCACTTTCTGAGTAACCTTGCAACTCAAAACTTTCAACTTTGCTAAATTTGATCCTAAAAGACAATGTGCCTTTTAGATTCTTAGAACGCTTTTTGTAGCAACCATGTGATTTTTACTTGGACATTCTTATCTGTACTTCTGTACTTTGCTAAATTTGATCCCAAAAGACAATGTGCCTTTCAGATATCTTAGAGAGATATACAACTAGACTTCTGTACACATTCTCATCTGCAGGTTCCGCTCCACCAGCCTTTTGCAACTTATCCTTTTGAATCATTGGAGTGTTCACACCCTTACACTCCTCCATATTGAACTTCTTCAATATCTCTCTCATGTACTTCTTTTAACAAAGAAACACTTCATTTTGTATTTGTTGAATCTCCATACCTAGGAAGTAAGACCTAGATCAGTCATCTCAAATACCTTCATCATTTCTTGTTTGAACACCTCTATTTGAGCACCATCACTTCTTGTCACTAGCAAGTCATCTACAGAGATACAATCCCAATATCAATACATTCTTCACATATAGTGGGGATTCACTTAAACTCTTTACAAAACCCAAGTTGTGTTCATCCATCTTGCTATACCATGCCCTAAGAGCTTGCTTCAACCCGTAGAAAGTCTTCTTCAACAAATAAACCTTCTACTCTTGCCTTGATGTGATGAACCCATTTGGTTGCTCAATGTAGATCTCTTCTTCTAACACTCCATTCAGAAATGCTAACTTCACATCCAGTTGATACACCTTCCAACCATGTTGAGCTAACAGAACAAGCACTAATTTGATTATGTCCATCCTTGCAATAGGTGCAAAAGTATAAGAGAAATTAGCTCCCCAGACTTGTGCATACCATTTCACCACTAATCTAACCTTGAGCTTGTTCACAGAGCAATCGGGATTCAGTTTGGTCTTGAAAACCCAGTTAACCCCAATCACTTTCCTGTTTACAGGCTTCTCCACCAACTCTCATGTTTTGTTCTTCTCTAACATTCTCAACTCCTCTTTCATTGCCTCCATCCACTTCTGATCTTGTTTAGCTTCTTCATACCCTTCAGGCTCCAATATAGCTACACTACTTCTCTCATACACATCATTCAATAGTCTAGTCCCTCTAAGTGGTGCATCATCAACCAGTTCATCAGGATCTAGAGAGACTTCTTTATGCTTCACCTCCACTTCTTCTGTCCAGTCCCACTCCTCATGCTCCAAGAATTGAACATCCCTACTAACCATCTTCATTGTGTGAGGATGATAAATCTTGTAGGCTTTGAACACCATGTTATAGCCTACAAAGGTTCTTGGTTTTGCCTTTTCACTCAACTTGTCTCTCTTCACATGTGGATATGAGAAAAGCACAAACAACCAAACACCTTCAAATTTTTCAATCTTGGTTTAGTATTAAACCAAGCTTTGTAAGGTATCTTTTTCTCTAATGCCTTTGTTGGTAGTCTATTCAATAGAAAGACTAATGTGGTAGCTGCTTCAGCCCAAAACTCTTTTGACAAGTTCTTCTCATGCAACATGCATCTTCTCATCTCACTGCTCCATATACTCCACAACAAAACGGTGTAAGTGAGAGGAAGAATAGGAGTATTATGGAGATGAAAAGATGCATTGTAAAAGTAGATTTGCACTTAGTTAACTAACAACCTCACATGACTTTTTAATTGTTTTTTTTTAACCTTACATGACTTTTTAATTGTTTTTTTTAAATCTAAACAAGTTGATTATTCTTAACACCAACCCCAACTAGAGGACACACTTTGTCTGACCCTAAGATAGTGTGCATTATAACTTTTGTGTTCAAATTTTTGTAAACTACACTTGGTGTGCCTTTTGGAAAAGGCGTTTCAAGTGAAGCACATATAATGGAATCAGTTTTAGAAGAAAATTAGTTAAATGCGTCCTCTTTAAGTACTCTATGTACTTTTTACTAATTTTTAGAATTTCTCAAACTACTTTTGCTTATTGAAAACTTTTTAAAATGCTTTTAATTGGTCGAAAACACTTTTTACCACAACATTTTAATCAAATACTACCAGTCTCTAATTAAGTACTTTCAATCATCCACAAGGACTTTAAAAGGCATGTCAAAGACATTTAGATTAGTACTCAATTGTATGGTTGTGAAATTGTTTATGCAATGTATCAATTTCATTTTTAGCCATTATTGTAATCCAATATCGAATAATAGGCTTCAACTTGTGTCGTTTCTTTCTTCTGGAAAATTCTATACATTACTTAGAATTTATCTGATCCTTTAATTCCTGTGATGTAAATTATTTCTTTTACAGGGGATATCCACCTTCAAAAAAAGATATTGAAGTTCTTGAGAGAACATCCAGATGCATTCCAGTGAAATATTGTCATGTTGAACATGGACGTGTCTGTTTCTTTTCATTCGATAATGTGGAGCTCCCCATCTTACCTTGACATTATTGTGGATTGCTCTGCTATTTTATTTTTGATATGTTATTTGAATTCTATTTTATGTTAAAAAAGAGTGTTTGGTCTGGTGGGCAAATTGATAAGTTGAAAGTGTACTTATTTTTAAATCTGATTTTACAGTCCTC

mRNA sequence

ATGGAGGCTACGGATTGGGAAAGCTCTTCAGGAGGAGCCAGTGGTGATGACGACAATTACGAGCAAGACATAAAAGATGAAGAAGAATGTCTCTGCGCATCTGGGTGCTTGCGCAAGTTGCAATTCAGGAAGCATGCTTCAACTGCTCGATGGAACGATCAAATGGGAATGGCAGAAGTTTTAGAGAACAAGGGCAGCCTTTGGACGACAACTGGCATTGTACGGTCATATCATGACAAAGAGAAGAAATTGAAAGTGTTTCCAAGCCAAGGCCAGGAAATGGCCTATGAGAGCCGCATTAGATTTCTTATTGAAGTTGGGGCCTTAGATCTTTTGGATCATGATAATTCAAGTCTTTCTTTGAAAGATGTATATAAGAAGGTAGCTGAAGGAAAAAGTGGGTGTCTTTGGGAGCAGTTCGAGGTTTATAGGCACCTCAAATCTCTTGGTTTCATTGTTGGAAAGCATAAAGTTCCTTGGTCTCTGAAGAGTGTTAGGAATGACAGTGACGTTTCATCTCCAAGTTCTATTGAAAACAAAGGAGCATCTGATGTCAAATCAGAAGATGAGAGGGGATATCCACCTTCAAAAAAAGATATTGAAGTTCTTGAGAGAACATCCAGATGCATTCCAGTGAAATATTGTCATGTTGAACATGGACGTGTCTGTTTCTTTTCATTCGATAATGTGGAGCTCCCCATCTTACCTTGACATTATTGTGGATTGCTCTGCTATTTTATTTTTGATATGTTATTTGAATTCTATTTTATGTTAAAAAAGAGTGTTTGGTCTGGTGGGCAAATTGATAAGTTGAAAGTGTACTTATTTTTAAATCTGATTTTACAGTCCTC

Coding sequence (CDS)

ATGGAGGCTACGGATTGGGAAAGCTCTTCAGGAGGAGCCAGTGGTGATGACGACAATTACGAGCAAGACATAAAAGATGAAGAAGAATGTCTCTGCGCATCTGGGTGCTTGCGCAAGTTGCAATTCAGGAAGCATGCTTCAACTGCTCGATGGAACGATCAAATGGGAATGGCAGAAGTTTTAGAGAACAAGGGCAGCCTTTGGACGACAACTGGCATTGTACGGTCATATCATGACAAAGAGAAGAAATTGAAAGTGTTTCCAAGCCAAGGCCAGGAAATGGCCTATGAGAGCCGCATTAGATTTCTTATTGAAGTTGGGGCCTTAGATCTTTTGGATCATGATAATTCAAGTCTTTCTTTGAAAGATGTATATAAGAAGGTAGCTGAAGGAAAAAGTGGGTGTCTTTGGGAGCAGTTCGAGGTTTATAGGCACCTCAAATCTCTTGGTTTCATTGTTGGAAAGCATAAAGTTCCTTGGTCTCTGAAGAGTGTTAGGAATGACAGTGACGTTTCATCTCCAAGTTCTATTGAAAACAAAGGAGCATCTGATGTCAAATCAGAAGATGAGAGGGGATATCCACCTTCAAAAAAAGATATTGAAGTTCTTGAGAGAACATCCAGATGCATTCCAGTGAAATATTGTCATGTTGAACATGGACGTGTCTGTTTCTTTTCATTCGATAATGTGGAGCTCCCCATCTTACCTTGA

Protein sequence

MEATDWESSSGGASGDDDNYEQDIKDEEECLCASGCLRKLQFRKHASTARWNDQMGMAEVLENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLLDHDNSSLSLKDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLKSVRNDSDVSSPSSIENKGASDVKSEDERGYPPSKKDIEVLERTSRCIPVKYCHVEHGRVCFFSFDNVELPILP

Homology

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BLAST of Lsi03G015550 vs. TrEMBL
Match: A0A0A0KXV5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G334710 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 8.4e-87
Identity = 181/281 (64.41%), Postives = 198/281 (70.46%), Query Frame = 1


Query: 1   MEATDWESSSGGAS--GDDDNYEQDIKDEEECLCASGCLRKLQFRKHASTARWNDQMGMA 60
           MEAT+WESSSGGAS  GD+DNYEQDI DEEECL ASGCLRKLQFRKHASTARWNDQMGMA
Sbjct: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60

Query: 61  EVLENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLLDHDNSS 120
           EVLENKGSLWTTTGIVR         K++ S  + +       FLIEVGAL LLDHDNSS
Sbjct: 61  EVLENKGSLWTTTGIVRCG-------KIYCSIEETL-------FLIEVGALHLLDHDNSS 120

Query: 121 LSLKDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLKSVRNDSDVSSPSSIE 180
           LSLKDVYKKVAEG+SG LWEQFEVYRHLKSLG+IVGKH+VPWSLK+VRND D+SSPSS E
Sbjct: 121 LSLKDVYKKVAEGRSGRLWEQFEVYRHLKSLGYIVGKHRVPWSLKNVRNDCDISSPSSTE 180

Query: 181 NKGASDVKSEDERGYPPSKKDIEVLER--------------------------------- 237
           NKGASDVKS+DE+        I++ E                                  
Sbjct: 181 NKGASDVKSDDEQSICRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPP 240

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BLAST of Lsi03G015550 vs. TrEMBL
Match: A0A059D747_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B02734 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.4e-49
Identity = 123/278 (44.24%), Postives = 153/278 (55.04%), Query Frame = 1


Query: 1   MEATDWESSSGGASGDDDNYEQDIKDEEECLCASGCLRKLQFRKHASTARWNDQMGMAEV 60
           ME  DW+    G S D +  EQD+KDEEE    S    KLQFRK  S ARWN ++ MAEV
Sbjct: 1   MECEDWD----GLSSDSELLEQDLKDEEEYNYTSNSSSKLQFRKVTSVARWNSRVRMAEV 60

Query: 61  LENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLLDHDNSSLS 120
           +E KG LWTTTGIVRS              G+         FL+E+GAL LL  ++ S+S
Sbjct: 61  VERKGQLWTTTGIVRS--------------GKLYCSIEETLFLMEIGALLLLSENDESIS 120

Query: 121 LKDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLKSV-RNDSDVSS---PSS 180
           L+ +Y+++AE K GC WE +EVYRHLKSLG+IVG+H +PWS+KSV RN   VSS     S
Sbjct: 121 LETMYQQLAEEKDGCFWELYEVYRHLKSLGYIVGRHGIPWSMKSVKRNTGTVSSEGTSES 180

Query: 181 IENKGASDVKSEDE--------------------------------------RGYPPSKK 237
           +E +   D  S  E                                      RG PPSK 
Sbjct: 181 LEVQSPQDCNSVIERLIKMDLNELKLVFDVYLPNGKFRKSSPGDPSYILSLIRGQPPSKI 240

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BLAST of Lsi03G015550 vs. TrEMBL
Match: A0A059DI98_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02322 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.1e-46
Identity = 120/278 (43.17%), Postives = 148/278 (53.24%), Query Frame = 1


Query: 1   MEATDWESSSGGASGDDDNYEQDIKDEEECLCASGCLRKLQFRKHASTARWNDQMGMAEV 60
           ME  DW     G S D +  E+D+KDEEE    S    KLQFRK  S ARWN  +GMAEV
Sbjct: 1   MECEDWH----GLSSDSELLEEDLKDEEEYNYTSNSSSKLQFRKVTSVARWNSGVGMAEV 60

Query: 61  LENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLLDHDNSSLS 120
           +E KG LW TTGIV S              G+         FL+E+GAL LL  ++ S+S
Sbjct: 61  VERKGQLWMTTGIVHS--------------GKLYCSIEETLFLMEIGALLLLSENDESIS 120

Query: 121 LKDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLKSV-RNDSDVSS---PSS 180
           L+ +Y+++AE K GC WE +EVYRHLKSLG+IVG+H +PWSLKSV +N   VSS     S
Sbjct: 121 LETMYQQLAEEKDGCFWELYEVYRHLKSLGYIVGRHGIPWSLKSVKKNTGTVSSEGTSES 180

Query: 181 IENKGASDVKSEDERG--------------------------------------YPPSKK 237
           +E +   D  S  ER                                        PPSK 
Sbjct: 181 LEVQSPQDCNSVIERFIKMDLNELKLVFDVYLPNGKFRKSSPGDPSYVLSLIRGQPPSKI 240

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BLAST of Lsi03G015550 vs. TrEMBL
Match: V4S2K6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005938mg PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 5.1e-44
Identity = 115/285 (40.35%), Postives = 156/285 (54.74%), Query Frame = 1


Query: 2   EATDWESSSGGASGDDDNYEQDIKDEEECLCASGCLRKLQFRKHASTARWNDQMGMAEVL 61
           +   WE     AS ++D+Y  +I +E+  +  SG + KLQFRK  S A WN+++GMAEV+
Sbjct: 5   DCDSWEK----ASDNEDSYVDEIDEEDYYM--SGFISKLQFRKDISKAHWNNELGMAEVV 64

Query: 62  ENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLLDHDNSSLSL 121
           E KG +WTTTGIVR+              G+         FL E+GAL LLD+++  L L
Sbjct: 65  EKKGKMWTTTGIVRN--------------GKTYCSIEETLFLAEIGALYLLDNNDLCLPL 124

Query: 122 KDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLK--SVRNDSDVSSPSSIE- 181
           K++Y+K+A  KSGC WE FEVYRHLKSLG+IVG+H VPW +K    R+ +  S P S++ 
Sbjct: 125 KEIYEKIANEKSGCSWELFEVYRHLKSLGYIVGRHGVPWIVKIPKGRDINITSDPVSLQV 184

Query: 182 ---NKGASDVKSEDERG-------------------YPPSKK------------------ 237
                G  +V+ ++E                     Y P++K                  
Sbjct: 185 TPKRHGVMEVEPKEESSLVALFDNIQINEVRPVFDVYLPNRKFKKSCPGDPSFLLYLTCK 244

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BLAST of Lsi03G015550 vs. TrEMBL
Match: A0A0B0MEI8_GOSAR (tRNA-splicing endonuclease subunit Sen54 OS=Gossypium arboreum GN=F383_20012 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 6.3e-42
Identity = 113/280 (40.36%), Postives = 145/280 (51.79%), Query Frame = 1


Query: 1   MEATDWESSSGGASGDDDNYEQDIKDEEECLCASGCLRKLQFRKHASTARWNDQMGMAEV 60
           M + DW S S       D  E D  DEE+C  +SG L KLQFRK  S ARW D + MAEV
Sbjct: 1   MGSDDWGSCSDNEVYLQDRNEDD--DEEDCY-SSGFLSKLQFRKDISKARWVDDLAMAEV 60

Query: 61  LENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLLDHDNSSLS 120
           +E KG +W T GIVR+              G    +     FLIE+GAL +LD ++  LS
Sbjct: 61  VEKKGKMWVTMGIVRN--------------GNTYCFIEETLFLIEIGALHVLDGNDIHLS 120

Query: 121 LKDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLKSVRNDSDVSS-PSSIEN 180
           LK++Y+K++ GKSGC WE FEVY+HLKSLG++VG+H +PWS+K  +  S   S   S E+
Sbjct: 121 LKELYEKLSNGKSGCYWELFEVYKHLKSLGYVVGRHGIPWSVKGQQIKSGTCSLEGSQES 180

Query: 181 KGASDVKSEDERG-------------------YPPSKKD--------------------- 237
               +++ +DE                     Y P+ K                      
Sbjct: 181 NEMLELEPKDENSVIELFNNMQITDVSPAFNVYLPNSKFRKSSPGDPSFVLYISRCSPSS 240

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BLAST of Lsi03G015550 vs. TAIR10
Match: AT3G57360.1 (AT3G57360.1 unknown protein)

HSP 1 Score: 137.9 bits (346), Expect = 8.1e-33
Identity = 93/272 (34.19%), Postives = 138/272 (50.74%), Query Frame = 1


Query: 1   MEATDWESSSGGASGDDDNYEQDIKDEEECLCASGCLRKLQFRKHASTARWNDQMGMAEV 60
           ME  DWE+SS  +S ++  +  D  D+EE   + G + KLQFR  +S ARW  ++GMAEV
Sbjct: 1   MEEKDWEASS--SSENEGGFPND--DDEE-FHSGGSVPKLQFRVGSSKARWITELGMAEV 60

Query: 61  LENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLL-DHDNSSL 120
              +G LWTTTGI+RS              G+   +     +L E+G L +L + D+  +
Sbjct: 61  EVKRGKLWTTTGIIRS--------------GKTYCFIEEALYLSEIGELQILGNEDDIVI 120

Query: 121 SLKDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLK-SVRNDSDVSSPSSIE 180
            LKD+Y+K+AE KSGC WE +EVYR+LK LG+I+G+H V W+LK + R + +  S  + E
Sbjct: 121 PLKDLYEKIAEEKSGCSWENYEVYRYLKGLGYILGRHGVSWTLKDAARPNGEEESACAGE 180

Query: 181 NKGASD--VKSEDERGYPPSKKDIEVLERTSR---------------------------- 236
               +D   K   +     +K   +V    SR                            
Sbjct: 181 CPADNDTVTKLLGDMQICDAKAVFDVYLPNSRFKKSSPGEPSFVACFSGDSPPSKEDIKV 240

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BLAST of Lsi03G015550 vs. TAIR10
Match: AT3G02370.2 (AT3G02370.2 unknown protein)

HSP 1 Score: 102.1 bits (253), Expect = 4.9e-22
Identity = 52/122 (42.62%), Postives = 72/122 (59.02%), Query Frame = 1


Query: 57  MAEVLENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLL-DHD 116
           MAEV   +G LWTTTGI+R+              G+   +     +L E+G L LL D D
Sbjct: 1   MAEVEVKRGKLWTTTGIIRT--------------GKTYCFIEEALYLSEIGELQLLGDED 60

Query: 117 NSSLSLKDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLKSVRNDSDVSSPS 176
           +  +SLKD+Y ++AEGK GC WE +EVYR+LK LG+I+G+H VPW+ K   N +      
Sbjct: 61  DVVISLKDLYGEIAEGKYGCCWENYEVYRYLKGLGYILGRHGVPWTTKYAVNTTPSDEDE 108

Query: 177 SI 178
           S+
Sbjct: 121 SL 108

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BLAST of Lsi03G015550 vs. NCBI nr
Match: gi|778694019|ref|XP_011653726.1| (PREDICTED: uncharacterized protein LOC101210680 isoform X1 [Cucumis sativus])

HSP 1 Score: 328.2 bits (840), Expect = 1.2e-86
Identity = 181/281 (64.41%), Postives = 198/281 (70.46%), Query Frame = 1


Query: 1   MEATDWESSSGGAS--GDDDNYEQDIKDEEECLCASGCLRKLQFRKHASTARWNDQMGMA 60
           MEAT+WESSSGGAS  GD+DNYEQDI DEEECL ASGCLRKLQFRKHASTARWNDQMGMA
Sbjct: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60

Query: 61  EVLENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLLDHDNSS 120
           EVLENKGSLWTTTGIVR         K++ S  + +       FLIEVGAL LLDHDNSS
Sbjct: 61  EVLENKGSLWTTTGIVRCG-------KIYCSIEETL-------FLIEVGALHLLDHDNSS 120

Query: 121 LSLKDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLKSVRNDSDVSSPSSIE 180
           LSLKDVYKKVAEG+SG LWEQFEVYRHLKSLG+IVGKH+VPWSLK+VRND D+SSPSS E
Sbjct: 121 LSLKDVYKKVAEGRSGRLWEQFEVYRHLKSLGYIVGKHRVPWSLKNVRNDCDISSPSSTE 180

Query: 181 NKGASDVKSEDERGYPPSKKDIEVLER--------------------------------- 237
           NKGASDVKS+DE+        I++ E                                  
Sbjct: 181 NKGASDVKSDDEQSICRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPP 240

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BLAST of Lsi03G015550 vs. NCBI nr
Match: gi|659126611|ref|XP_008463274.1| (PREDICTED: uncharacterized protein LOC103501474 [Cucumis melo])

HSP 1 Score: 322.8 bits (826), Expect = 5.1e-85
Identity = 178/281 (63.35%), Postives = 191/281 (67.97%), Query Frame = 1


Query: 1   MEATDWESSSGGAS--GDDDNYEQDIKDEEECLCASGCLRKLQFRKHASTARWNDQMGMA 60
           MEAT+W+SSSGGAS  GD+DNYEQDI DEEECL AS CLRKLQFRKHASTARWNDQMGMA
Sbjct: 1   MEATNWKSSSGGASDTGDEDNYEQDINDEEECLSASRCLRKLQFRKHASTARWNDQMGMA 60

Query: 61  EVLENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLLDHDNSS 120
           EVLENKGSLWTTTGIVR               G+         FLIEVGAL LLDHDNS+
Sbjct: 61  EVLENKGSLWTTTGIVRC--------------GKIYCTIEETLFLIEVGALHLLDHDNST 120

Query: 121 LSLKDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLKSVRNDSDVSSPSSIE 180
           LSLKDVYKKVAEG+SGC+WEQFEVYRHLKSLG+IVGKHKVPWSLK+VRND D+SSPSS E
Sbjct: 121 LSLKDVYKKVAEGRSGCIWEQFEVYRHLKSLGYIVGKHKVPWSLKNVRNDCDLSSPSSTE 180

Query: 181 NKGASDVKSEDE----------------------------RGYPPSKKDIEVLER----- 237
            KG SDVKSEDE                            R   P   +  V        
Sbjct: 181 KKGVSDVKSEDEQLISGLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLARGYPP 240

[Close]

BLAST of Lsi03G015550 vs. NCBI nr
Match: gi|702274046|ref|XP_010044016.1| (PREDICTED: tRNA-splicing endonuclease subunit Sen54-like [Eucalyptus grandis])

HSP 1 Score: 203.8 bits (517), Expect = 3.4e-49
Identity = 123/278 (44.24%), Postives = 153/278 (55.04%), Query Frame = 1


Query: 1   MEATDWESSSGGASGDDDNYEQDIKDEEECLCASGCLRKLQFRKHASTARWNDQMGMAEV 60
           ME  DW+    G S D +  EQD+KDEEE    S    KLQFRK  S ARWN ++ MAEV
Sbjct: 1   MECEDWD----GLSSDSELLEQDLKDEEEYNYTSNSSSKLQFRKVTSVARWNSRVRMAEV 60

Query: 61  LENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLLDHDNSSLS 120
           +E KG LWTTTGIVRS              G+         FL+E+GAL LL  ++ S+S
Sbjct: 61  VERKGQLWTTTGIVRS--------------GKLYCSIEETLFLMEIGALLLLSENDESIS 120

Query: 121 LKDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLKSV-RNDSDVSS---PSS 180
           L+ +Y+++AE K GC WE +EVYRHLKSLG+IVG+H +PWS+KSV RN   VSS     S
Sbjct: 121 LETMYQQLAEEKDGCFWELYEVYRHLKSLGYIVGRHGIPWSMKSVKRNTGTVSSEGTSES 180

Query: 181 IENKGASDVKSEDE--------------------------------------RGYPPSKK 237
           +E +   D  S  E                                      RG PPSK 
Sbjct: 181 LEVQSPQDCNSVIERLIKMDLNELKLVFDVYLPNGKFRKSSPGDPSYILSLIRGQPPSKI 240

[Close]

BLAST of Lsi03G015550 vs. NCBI nr
Match: gi|702247545|ref|XP_010056838.1| (PREDICTED: tRNA-splicing endonuclease subunit Sen54-like isoform X1 [Eucalyptus grandis])

HSP 1 Score: 194.9 bits (494), Expect = 1.6e-46
Identity = 120/278 (43.17%), Postives = 148/278 (53.24%), Query Frame = 1


Query: 1   MEATDWESSSGGASGDDDNYEQDIKDEEECLCASGCLRKLQFRKHASTARWNDQMGMAEV 60
           ME  DW     G S D +  E+D+KDEEE    S    KLQFRK  S ARWN  +GMAEV
Sbjct: 1   MECEDWH----GLSSDSELLEEDLKDEEEYNYTSNSSSKLQFRKVTSVARWNSGVGMAEV 60

Query: 61  LENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLLDHDNSSLS 120
           +E KG LW TTGIV S              G+         FL+E+GAL LL  ++ S+S
Sbjct: 61  VERKGQLWMTTGIVHS--------------GKLYCSIEETLFLMEIGALLLLSENDESIS 120

Query: 121 LKDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLKSV-RNDSDVSS---PSS 180
           L+ +Y+++AE K GC WE +EVYRHLKSLG+IVG+H +PWSLKSV +N   VSS     S
Sbjct: 121 LETMYQQLAEEKDGCFWELYEVYRHLKSLGYIVGRHGIPWSLKSVKKNTGTVSSEGTSES 180

Query: 181 IENKGASDVKSEDERG--------------------------------------YPPSKK 237
           +E +   D  S  ER                                        PPSK 
Sbjct: 181 LEVQSPQDCNSVIERFIKMDLNELKLVFDVYLPNGKFRKSSPGDPSYVLSLIRGQPPSKI 240

[Close]

BLAST of Lsi03G015550 vs. NCBI nr
Match: gi|720076187|ref|XP_010279548.1| (PREDICTED: uncharacterized protein LOC104613432 [Nelumbo nucifera])

HSP 1 Score: 188.3 bits (477), Expect = 1.5e-44
Identity = 120/278 (43.17%), Postives = 155/278 (55.76%), Query Frame = 1


Query: 1   MEATDWESSSGGASGDDDNYEQDIKDEEECLCASGCLRKLQFRKHASTARWNDQMGMAEV 60
           ME  D  SSS G S D +   +D+ DEE     SG + KLQFRK  S ARWN +MGMAEV
Sbjct: 1   MEIEDLASSSDGMS-DLEVPSEDLNDEE-LQYTSGSIPKLQFRKDTSKARWNAEMGMAEV 60

Query: 61  LENKGSLWTTTGIVRSYHDKEKKLKVFPSQGQEMAYESRIRFLIEVGALDLLDHDNSSLS 120
           +E KG +W TTGIVR              +G+         FL E+GAL LLD D+++L 
Sbjct: 61  IEKKGKMWITTGIVR--------------RGKLYCTIEETLFLAEIGALHLLDVDDTTLF 120

Query: 121 LKDVYKKVAEGKSGCLWEQFEVYRHLKSLGFIVGKHKVPWSLK---------------SV 180
           LKD+Y+KVAEG+SGC WE FEVYRHLKSLG+IVG++ VPW++K               S 
Sbjct: 121 LKDLYEKVAEGRSGCCWETFEVYRHLKSLGYIVGRYGVPWTMKGDNGCNSTLQGTAETST 180

Query: 181 RNDSDVSSPSSI----ENKGASDVKSEDERGYPPSK-----------------------K 237
            +DS++ +  S     EN   SD++ + E   P +K                        
Sbjct: 181 VSDSELVNRFSFINQRENLQISDLRPDFEVYLPNNKFRKSSPGDPSFMLFVTRAHPPSRN 240

The following BLAST results are available for this feature:

BLAST of Lsi03G015550 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Lsi03G015550 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXV5_CUCSA	8.4e-87	64.41	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G334710 PE=4 SV=1	[more]
A0A059D747_EUCGR	2.4e-49	44.24	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B02734 PE=4 SV=1	[more]
A0A059DI98_EUCGR	1.1e-46	43.17	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02322 PE=4 SV=1	[more]
V4S2K6_9ROSI	5.1e-44	40.35	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005938mg PE=4 SV=1	[more]
A0A0B0MEI8_GOSAR	6.3e-42	40.36	tRNA-splicing endonuclease subunit Sen54 OS=Gossypium arboreum GN=F383_20012 PE=...	[more]

BLAST of Lsi03G015550 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G57360.1	8.1e-33	34.19	unknown protein	[more]
AT3G02370.2	4.9e-22	42.62	unknown protein	[more]

BLAST of Lsi03G015550 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778694019\|ref\|XP_011653726.1\|	1.2e-86	64.41	PREDICTED: uncharacterized protein LOC101210680 isoform X1 [Cucumis sativus]	[more]
gi\|659126611\|ref\|XP_008463274.1\|	5.1e-85	63.35	PREDICTED: uncharacterized protein LOC103501474 [Cucumis melo]	[more]
gi\|702274046\|ref\|XP_010044016.1\|	3.4e-49	44.24	PREDICTED: tRNA-splicing endonuclease subunit Sen54-like [Eucalyptus grandis]	[more]
gi\|702247545\|ref\|XP_010056838.1\|	1.6e-46	43.17	PREDICTED: tRNA-splicing endonuclease subunit Sen54-like isoform X1 [Eucalyptus ...	[more]
gi\|720076187\|ref\|XP_010279548.1\|	1.5e-44	43.17	PREDICTED: uncharacterized protein LOC104613432 [Nelumbo nucifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR024337	tRNA_splic_suSen54

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G015550.1	Lsi03G015550.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024337	tRNA-splicing endonuclease, subunit Sen54	PANTHER	PTHR21027	TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54	coord: 39..179 score: 7.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G015550	Csa3G822480	Cucumber (Chinese Long) v2	culsiB186
Lsi03G015550	CSPI03G38330	Wild cucumber (PI 183967)	cpilsiB184
Lsi03G015550	Cla002926	Watermelon (97103) v1	lsiwmB240
Lsi03G015550	ClCG10G015700	Watermelon (Charleston Gray)	lsiwcgB219
Lsi03G015550	MELO3C030712.2	Melon (DHL92) v3.6.1	lsimedB273
Lsi03G015550	Cla97C10G198600	Watermelon (97103) v2	lsiwmbB218
Lsi03G015550	Bhi11G002396	Wax gourd	lsiwgoB303
Lsi03G015550	CsGy3G035870	Cucumber (Gy14) v2	cgyblsiB171
Lsi03G015550	CsaV3_3G040730	Cucumber (Chinese Long) v3	cuclsiB203

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G015550	Silver-seed gourd	carlsiB292
Lsi03G015550	Cucumber (Gy14) v1	cgylsiB551
Lsi03G015550	Cucurbita maxima (Rimu)	cmalsiB242
Lsi03G015550	Cucurbita maxima (Rimu)	cmalsiB753
Lsi03G015550	Cucurbita moschata (Rifu)	cmolsiB230
Lsi03G015550	Cucurbita moschata (Rifu)	cmolsiB745
Lsi03G015550	Cucurbita pepo (Zucchini)	cpelsiB503
Lsi03G015550	Melon (DHL92) v3.5.1	lsimeB260