Lsi03G015200 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G015200
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	TRNA/rRNA methyltransferase family protein
Location	chr03 : 26209028 .. 26212043 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTCTTCCTAGTTCCCGGCTTGCTTTTCTACCACTGTCTGAACCATCGTAGCTCTTCAACGTACAGCCATGGCGTCCTACAGAATTGTAGCCCGTTCTTCTCTTATTCTGCTGGAAAGCTGCAGTCCCCGAAGACCTCAGCGAATCTTCCCTTCTGTTTCTCTCCCCTCTCTACCTCTTCCTTCCTCCTCTCCGTTCTTCACCATCGTCACTAACCCACGTACCGTCCACTCTCTCTCTCTCTCTCTCGCTTGAAGGCCATACAAATCTTTACATTTCTTTTATAAATCTGTTTTTTATTTTCCTTCTGTTTTGATTGCTATATAGTCTCAAGAAGCAGCCCTAACGCAGTTTCTACAAGATGTCTTGCTTCAATTTCGGCCGCAACATCTTCAATAGAAGTAGCAGATACTGTGGGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACGCACGCCGATGACGTTACCAGGTTGATGAAAATGGAGCGGAGGCCGCTTGAGTTAGATGGAGCTGAATATCCACATAAAGGGCGGCGGTTTCCGTACCTGGATAAGTTTAGCTGTGGAAGTTCTTGGTTAAATAGTGGTGAGATTTTGGAGGCCTTGGATCCTCTCATACTGGACTCGAGGAAAAAGCGGTTCAAGAACGTGGTGCAGAACAGAAGCTACTCGGTTTGCTTGGTGGTGGAGGGATTAAGCGATTTCGGCAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAACAACCATGGAGGTGAAATTGTTTTCGAATGCATTTGTTCCTTTATTTCTTATGGGACAATTGTTTGATTCTAGAATTTGGCAGCTGCTTTGTTTCCATTGTGCTGGCGGAATTTCATACTGTTCTTGGTGCTTTTACTTTGTTGTTCAATGGGTCACTTGGATCCAATTTATTAGTCGAATGCCATTGCTGGTTTCATATGGTTAGAAGGTTAAATTGATCAAATACGTTTCATTTTCATTGGTAATTGTTAGATATGGGTGGCAGTGTAACACAAATTGAGCAATGGAATGGAGTGTTTTCTTCCTGCAACTATGCATCTCTTGTATAGTTACAAGTATATATTATACTTTTATATGTATATATGTATATGTGTGTATTTTTATATGTATATAATCATTGGAAACTGTATACCAAATACCAATTAAAATGTATTATTTTACATTGCACAACGTTTCTTTTCATGTTGATTTTCCATGTGAAATGTACATGTTCATATGTTTTTTCTTCCTTCATTTGCTATCTTTATCTCGCCATTTCATGACAGTAAGTGCTGTTTGTCTTGGACATGAAACTTTAACCAGTGCTTGTATTTTCATGCTTATCCTGAATGAGATGATGCTTTGATAAATTAGGGTGCAACAGGGGTGAGGTTACATATATTGCCTATGTGGAAAGAGCTACTTCTATTTAATGGGTTCATTCAACTTGCCAAAAATGACAAAAGTCCCCATTTCTTACATTTTCTTTGTGGATTTTATTCCTGTTTTTCTTCGTCTAACATAATTGCAATTCGAAGCAGGTACAGAGAAAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCCACCCCAGAATGTTTTAAAATTCTGAAATCTCGCGGTTATAGAATAGTCTCTACGCATCTAGGAACGAATGCGGTATTGATCAATTTTGTACTTTCCCTCTTGTCACATTAAAACTTTTGTAAGCTTGTCCTGACAGCTGAGTTATGATTGTCCTGTTGTAGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTGGGGAATGAAAGTAGGTAATTATCTTCTTGCTCCAATAATATGGAGTGTGATCAAGTTTAGACCACTTTAAGTTATGGAATGATTGCAACAGTTGTTTTCTCTTTGTTATTTGATGCACTTTTTTGAACCACTGCAAGTTGGGAGATATCAGTTAAATTTACGAAATCAAAACTGAATCCACGTTTAAAAGGCATACACTGCCATTATCCAACAATTCTTTGAAATCTACAATTATCTCATGCTTCAGTTAGCAAGTTTCGACGCATGAATGATGTCCGAATCAGGAGAAATTATACTTGTAGCTAGTTATAACCTTCACAATTTATGTTGGTTTCTGAGGTTAAGATTTCTTCCTTCTTCAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGACCGCACTTCTCGACTGGTAACTAGAAAAAAAAAAGGCGTTTTTGATACTTGGATTCATTATGCCATAAGGGCTTTGTGTCGAATTTTATTGCCTGGATAGCTCAATATGGAGAATTCTCAATTGTCTCTCGCAGCTGGGGATGTGGGCTGAGCTAGTATTTCCGTCGATAGGGAGGATGTCAAAAATAGATATATATACTGCTTCTATCATACAATTGATGGACTATGTTCTTTATCAAACTTTTCAGGGCTGTCACGGAGATCTTACATCAGAAGAAAGACAAATTCTACTTGCGGAGTTCTCTCTAAGGCATAGCAAGAATGCAATCAGCATTGCGAACGAGTTGGCAAAGCGGAAGGGTTCCGTGATCTCTGGTACCGACGTCTTGCTCTAGCATGGCAAGAATTTGGCGTCGTGATGTGTCTGGGAAATGTTTATGAGAAGCAGAAACACACAGAAGAAGCCCCCCCTTCAAAGTTTGATTAAAACAGGTAAAATTTCTAAAGGAACCTGGCTCAGAAAATTTGCAACAAGTACAATTTCAAAGCAGTTTAGTATCAATTCATATGGCTGTATAATGTTGTTGAAGTTCTGTATTTGACTTGGAGAAACGTTTACCTTAGTTGTTCAGCATCA

mRNA sequence

TTTTCTTCCTAGTTCCCGGCTTGCTTTTCTACCACTGTCTGAACCATCGTAGCTCTTCAACGTACAGCCATGGCGTCCTACAGAATTGTAGCCCGTTCTTCTCTTATTCTGCTGGAAAGCTGCAGTCCCCGAAGACCTCAGCGAATCTTCCCTTCTGTTTCTCTCCCCTCTCTACCTCTTCCTTCCTCCTCTCCGTTCTTCACCATCGTCACTAACCCACTCTCAAGAAGCAGCCCTAACGCAGTTTCTACAAGATGTCTTGCTTCAATTTCGGCCGCAACATCTTCAATAGAAGTAGCAGATACTGTGGGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACGCACGCCGATGACGTTACCAGGTTGATGAAAATGGAGCGGAGGCCGCTTGAGTTAGATGGAGCTGAATATCCACATAAAGGGCGGCGGTTTCCGTACCTGGATAAGTTTAGCTGTGGAAGTTCTTGGTTAAATAGTGGTGAGATTTTGGAGGCCTTGGATCCTCTCATACTGGACTCGAGGAAAAAGCGGTTCAAGAACGTGGTGCAGAACAGAAGCTACTCGGTTTGCTTGGTGGTGGAGGGATTAAGCGATTTCGGCAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAACAACCATGGAGAATTTGGCAGCTGCTTTGTTTCCATTGTGCTGGCGGAATTTCATACTGTTCTTGGTGCTTTTACTTTGTTGTTCAATGGGTCACTTGGATCCAATTTATTAGTCGAATGCCATTGCTGGTTTCATATGGTTAGAAGGTACAGAGAAAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCCACCCCAGAATGTTTTAAAATTCTGAAATCTCGCGGTTATAGAATAGTCTCTACGCATCTAGGAACGAATGCGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTGGGGAATGAAAGTAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGACCGCACTTCTCGACTGGGCTGTCACGGAGATCTTACATCAGAAGAAAGACAAATTCTACTTGCGGAGTTCTCTCTAAGGCATAGCAAGAATGCAATCAGCATTGCGAACGAGTTGGCAAAGCGGAAGGGTTCCGTGATCTCTGGTACCGACGTCTTGCTCTAGCATGGCAAGAATTTGGCGTCGTGATGTGTCTGGGAAATGTTTATGAGAAGCAGAAACACACAGAAGAAGCCCCCCCTTCAAAGTTTGATTAAAACAGGTAAAATTTCTAAAGGAACCTGGCTCAGAAAATTTGCAACAAGTACAATTTCAAAGCAGTTTAGTATCAATTCATATGGCTGTATAATGTTGTTGAAGTTCTGTATTTGACTTGGAGAAACGTTTACCTTAGTTGTTCAGCATCA

Coding sequence (CDS)

ATGGCGTCCTACAGAATTGTAGCCCGTTCTTCTCTTATTCTGCTGGAAAGCTGCAGTCCCCGAAGACCTCAGCGAATCTTCCCTTCTGTTTCTCTCCCCTCTCTACCTCTTCCTTCCTCCTCTCCGTTCTTCACCATCGTCACTAACCCACTCTCAAGAAGCAGCCCTAACGCAGTTTCTACAAGATGTCTTGCTTCAATTTCGGCCGCAACATCTTCAATAGAAGTAGCAGATACTGTGGGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACGCACGCCGATGACGTTACCAGGTTGATGAAAATGGAGCGGAGGCCGCTTGAGTTAGATGGAGCTGAATATCCACATAAAGGGCGGCGGTTTCCGTACCTGGATAAGTTTAGCTGTGGAAGTTCTTGGTTAAATAGTGGTGAGATTTTGGAGGCCTTGGATCCTCTCATACTGGACTCGAGGAAAAAGCGGTTCAAGAACGTGGTGCAGAACAGAAGCTACTCGGTTTGCTTGGTGGTGGAGGGATTAAGCGATTTCGGCAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAACAACCATGGAGAATTTGGCAGCTGCTTTGTTTCCATTGTGCTGGCGGAATTTCATACTGTTCTTGGTGCTTTTACTTTGTTGTTCAATGGGTCACTTGGATCCAATTTATTAGTCGAATGCCATTGCTGGTTTCATATGGTTAGAAGGTACAGAGAAAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCCACCCCAGAATGTTTTAAAATTCTGAAATCTCGCGGTTATAGAATAGTCTCTACGCATCTAGGAACGAATGCGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTGGGGAATGAAAGTAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGACCGCACTTCTCGACTGGGCTGTCACGGAGATCTTACATCAGAAGAAAGACAAATTCTACTTGCGGAGTTCTCTCTAAGGCATAGCAAGAATGCAATCAGCATTGCGAACGAGTTGGCAAAGCGGAAGGGTTCCGTGATCTCTGGTACCGACGTCTTGCTCTAG

Protein sequence

MASYRIVARSSLILLESCSPRRPQRIFPSVSLPSLPLPSSSPFFTIVTNPLSRSSPNAVSTRCLASISAATSSIEVADTVGDLEPPEIHTQDTVEHLLTHADDVTRLMKMERRPLELDGAEYPHKGRRFPYLDKFSCGSSWLNSGEILEALDPLILDSRKKRFKNVVQNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNHGEFGSCFVSIVLAEFHTVLGAFTLLFNGSLGSNLLVECHCWFHMVRRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKNAISIANELAKRKGSVISGTDVLL

Homology

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BLAST of Lsi03G015200 vs. Swiss-Prot
Match: TRMH_AQUAE (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) GN=trmH PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.6e-17
Identity = 47/135 (34.81%), Postives = 83/135 (61.48%), Query Frame = 1


Query: 262 RRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPT 321
           ++ + N  ++ G+ KW+ IE  ++  +     K+RG++IV+T L   +V+  ++D++ PT
Sbjct: 62  KKAKINEGITQGSHKWVFIEKVDNPVQKLLEFKNRGFQIVATWLSKESVNFREVDYTKPT 121

Query: 322 AIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR-LGCHG 381
            +VVGNE +G+S E +E++D    IPM GM  S NVSVA GI+++ A   R  + +    
Sbjct: 122 VLVVGNELQGVSPEIVEIADKKIVIPMYGMAQSLNVSVATGIILYEAQRQREEKGMYSRP 181

Query: 382 DLTSEERQILLAEFS 396
            L+ EE Q +L +++
Sbjct: 182 SLSEEEIQKILKKWA 196

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BLAST of Lsi03G015200 vs. Swiss-Prot
Match: TRMH_SHIFL (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri GN=trmH PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.8e-16
Identity = 53/155 (34.19%), Postives = 83/155 (53.55%), Query Frame = 1


Query: 263 RYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTA 322
           R R     + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT 
Sbjct: 56  RMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTC 115

Query: 323 IVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCH 382
           I++G E  GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +
Sbjct: 116 ILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLREN 175

Query: 383 GDLTSEERQILLAEFSLRHSKNAISIANELAKRKG 415
             L   E+Q LL E           +  ++AKRKG
Sbjct: 176 SMLPEAEQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of Lsi03G015200 vs. Swiss-Prot
Match: TRMH_ECOLI (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) GN=trmH PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.8e-16
Identity = 53/155 (34.19%), Postives = 83/155 (53.55%), Query Frame = 1


Query: 263 RYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTA 322
           R R     + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT 
Sbjct: 56  RMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTC 115

Query: 323 IVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCH 382
           I++G E  GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +
Sbjct: 116 ILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLREN 175

Query: 383 GDLTSEERQILLAEFSLRHSKNAISIANELAKRKG 415
             L   E+Q LL E           +  ++AKRKG
Sbjct: 176 SMLPEAEQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of Lsi03G015200 vs. Swiss-Prot
Match: TRMH_ECO57 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 GN=trmH PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.8e-16
Identity = 53/155 (34.19%), Postives = 83/155 (53.55%), Query Frame = 1


Query: 263 RYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTA 322
           R R     + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT 
Sbjct: 56  RMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTC 115

Query: 323 IVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCH 382
           I++G E  GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +
Sbjct: 116 ILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLREN 175

Query: 383 GDLTSEERQILLAEFSLRHSKNAISIANELAKRKG 415
             L   E+Q LL E           +  ++AKRKG
Sbjct: 176 SMLPEAEQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of Lsi03G015200 vs. Swiss-Prot
Match: TRMH_THET8 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=trmH PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.7e-14
Identity = 40/98 (40.82%), Postives = 61/98 (62.24%), Query Frame = 1


Query: 271 SMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESR 330
           S G+ KW+ + +     E F+ LK RG+ + +T L  +A    ++D++ PTA++ G E  
Sbjct: 67  SGGSHKWVYLRVHPDLHEAFRFLKERGFTVYATALREDARDFREVDYTKPTAVLFGAEKW 126

Query: 331 GISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHA 369
           G+S+EAL L+D    IPM GMV S NVSVAA +++  A
Sbjct: 127 GVSEEALALADGAIKIPMLGMVQSLNVSVAAAVILFEA 164

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BLAST of Lsi03G015200 vs. TrEMBL
Match: A0A0A0LC15_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G824750 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 2.0e-123
Identity = 268/428 (62.62%), Postives = 302/428 (70.56%), Query Frame = 1


Query: 1   MASYRIVARSSLILLESCSPRRPQRIFPSVSLPSLPLPSSSPFFTIVTNPLSRSSPNAVS 60
           MAS RIV+RSSL LL+SCSPRR QRIF SV LPSL LPS S FFT   N  SRS+PNAVS
Sbjct: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT---NRFSRSNPNAVS 60

Query: 61  TRCLASISAATSSIEVADTVGDLEPPEIHTQDTVEHLLTHADDVTRLMKMERRPLELDGA 120
           TRC ASISAATS +EV D V D+EPPE+ TQDTVEHLLTH++DV RLMKMERR +ELDGA
Sbjct: 61  TRCFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGA 120

Query: 121 EYPHKGRRFPYLDKFSCGSSWLNSGEILEALDPLILDSRKKRFKNVVQNRSYSVCLVVEG 180
           EYPHKGR FPYLDK+SCGSSWLNSGEILEALD LILDSRK+RFKNVV+NRSYSVCLVVEG
Sbjct: 121 EYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEG 180

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRC--NNHGEFGS-CFVSIVLAEFHTVLGA 240
           L+DFGNVSAAFRSADALGFQSVHVVSCDASKR   N H   G+  ++ I L  +++    
Sbjct: 181 LTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIEL--WNSTPEC 240

Query: 241 FTLLFNGSLGSNLLVECHCWFHMVRRYR-ENRHVSMGAEKWLDIELWNSTPECFKILKSR 300
           F +L +                  R YR  + H+   A    D++ W+         +SR
Sbjct: 241 FKILKS------------------RGYRIVSTHLGANAVSVYDMD-WSCPTAIVVGNESR 300

Query: 301 GYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFN 360
           G       +   A+ + D+  S P                            NGMVDSFN
Sbjct: 301 G-------ISNEALELSDLHCSIPM---------------------------NGMVDSFN 360

Query: 361 VSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKNAISIANELAKRKGSV 420
           VSVAAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSK+AISIANELAKRKGS 
Sbjct: 361 VSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSA 370

Query: 421 ISGTDVLL 425
           IS T+VLL
Sbjct: 421 ISSTEVLL 370

[Close]

BLAST of Lsi03G015200 vs. TrEMBL
Match: M5WSN7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008118mg PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.3e-74
Identity = 143/208 (68.75%), Postives = 166/208 (79.81%), Query Frame = 1


Query: 211 KRCNNHGEFGSCFVSIVLAEFHTVLGAFTLLFNGSLGSNLLVECHCWFHMVRRYRENRHV 270
           +R   +  +  C V   L +F  V  AF         S  ++ C       +RYR+NRHV
Sbjct: 138 RRVVKNRSYSVCLVVEGLGDFGNVSAAFRSADALGFQSVHVLSCDS----SKRYRDNRHV 197

Query: 271 SMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESR 330
           SMGAEKWLDIELW+STPECF++LKSRGYRI +TH+G +AVSVYDMDWSCPTAIVVGNE+R
Sbjct: 198 SMGAEKWLDIELWDSTPECFEVLKSRGYRIATTHVGMDAVSVYDMDWSCPTAIVVGNENR 257

Query: 331 GISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQIL 390
           GISDEAL+LSDLHCSIPM GMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLT EE QIL
Sbjct: 258 GISDEALDLSDLHCSIPMKGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTFEESQIL 317

Query: 391 LAEFSLRHSKNAISIANELAKRKGSVIS 419
           LAEFSLRHSK+++SIA+E AKRK +V++
Sbjct: 318 LAEFSLRHSKSSMSIAHEYAKRKAAVLT 341

[Close]

BLAST of Lsi03G015200 vs. TrEMBL
Match: A0A067FZF2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g018308mg PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 5.0e-74
Identity = 195/417 (46.76%), Postives = 242/417 (58.03%), Query Frame = 1


Query: 1   MASYRIVARSSLILLESCSPRRPQRIFPSVSLPSLPLPSSSPFFTIVTNPLSRSSPNAVS 60
           M++ R + R+S   +ES          PS S  SL L S  P       P+S   P +V 
Sbjct: 1   MSTCRAIIRTSFSFVES-----KFYFLPSKSFLSLSLRSQKP------GPIS---PYSVR 60

Query: 61  TRCLASISAATSSIEVADTVGDLEPPEIHTQDTVEHLLTHADDVTRLMKMERRPLELDGA 120
           TR   S+ AA S +E  + + D E  +  ++D VE LLT+ DDVT+ MKMER  +  DG 
Sbjct: 61  TRQYGSVQAAIS-LENGNNLIDDEN-DTPSKDNVEKLLTNPDDVTQFMKMERSCMVNDGV 120

Query: 121 EYPHKGRRFPYLDKFSCGSSWLNSGEILEALDPLILDSRKKRFKNVVQNRSYSVCL---- 180
                            GS        +E+L      S K+ F  + + +   V L    
Sbjct: 121 -----------------GS--------MESL------SNKRWFPYLDRYKCGDVYLSSSE 180

Query: 181 VVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNHGEFGSCFVSIVLAEFHTVLG 240
           VVE LS +       R  + +  +S  V                 C V   L++F  V  
Sbjct: 181 VVEALSPYLMEERKERFVNVVKNRSYSV-----------------CLVVEGLSDFGNVSA 240

Query: 241 AFTLLFNGSLGSNLLVECHCWFHMVRRYRENRHVSMGAEKWLDIELWNSTPECFKILKSR 300
            F       + S  +V C       +RYRENRHVSMGAEKWLDIELW++  ECF +L+SR
Sbjct: 241 TFRSADALGVQSVHVVSCDS----SKRYRENRHVSMGAEKWLDIELWDAPRECFNVLRSR 300

Query: 301 GYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFN 360
           GYRI +TH+G +A+SVYDMDWSCPTAIVVGNE+RG+SDEAL LSDL CSIPM GMVDSFN
Sbjct: 301 GYRIATTHVGMDAISVYDMDWSCPTAIVVGNENRGVSDEALSLSDLRCSIPMKGMVDSFN 349

Query: 361 VSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKNAISIANELAKRK 414
           VSVAAGILMHHAVCDR +RLGC+GDLTSEE+QILLAEFSLRHSK+AISI +E AKRK
Sbjct: 361 VSVAAGILMHHAVCDRATRLGCNGDLTSEEKQILLAEFSLRHSKSAISIVHEYAKRK 349

[Close]

BLAST of Lsi03G015200 vs. TrEMBL
Match: A0A067FZB2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g018308mg PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.2e-72
Identity = 195/418 (46.65%), Postives = 242/418 (57.89%), Query Frame = 1


Query: 1   MASYRIVARSSLILLESCSPRRPQRIFPSVSLPSLPLPSSSPFFTIVTNPLSRSSPNAVS 60
           M++ R + R+S   +ES          PS S  SL L S  P       P+S   P +V 
Sbjct: 1   MSTCRAIIRTSFSFVES-----KFYFLPSKSFLSLSLRSQKP------GPIS---PYSVR 60

Query: 61  TRCLASISAATSSIEVADTVGDLEPPEIHTQDTVEHLLTHADDVTRLMKMERRPLELDGA 120
           TR   S+ AA S +E  + + D E  +  ++D VE LLT+ DDVT+ MKMER  +  DG 
Sbjct: 61  TRQYGSVQAAIS-LENGNNLIDDEN-DTPSKDNVEKLLTNPDDVTQFMKMERSCMVNDGV 120

Query: 121 EYPHKGRRFPYLDKFSCGSSWLNSGEILEALDPLILDSRKKRFKNVVQNRSYSVCL---- 180
                            GS        +E+L      S K+ F  + + +   V L    
Sbjct: 121 -----------------GS--------MESL------SNKRWFPYLDRYKCGDVYLSSSE 180

Query: 181 VVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNHGEFGSCFVSIVLAEFHTVLG 240
           VVE LS +       R  + +  +S  V                 C V   L++F  V  
Sbjct: 181 VVEALSPYLMEERKERFVNVVKNRSYSV-----------------CLVVEGLSDFGNVSA 240

Query: 241 AFTLLFNGSLGSNLLVECHCWFHMVRRYRENRHVSMGAEKWLDIELWNSTPECFKILKSR 300
            F       + S  +V C       +RYRENRHVSMGAEKWLDIELW++  ECF +L+SR
Sbjct: 241 TFRSADALGVQSVHVVSCDS----SKRYRENRHVSMGAEKWLDIELWDAPRECFNVLRSR 300

Query: 301 GYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFN 360
           GYRI +TH+G +A+SVYDMDWSCPTAIVVGNE+RG+SDEAL LSDL CSIPM GMVDSFN
Sbjct: 301 GYRIATTHVGMDAISVYDMDWSCPTAIVVGNENRGVSDEALSLSDLRCSIPMKGMVDSFN 350

Query: 361 VSVAAGILMHHAVCDRTSRL-GCHGDLTSEERQILLAEFSLRHSKNAISIANELAKRK 414
           VSVAAGILMHHAVCDR +RL GC+GDLTSEE+QILLAEFSLRHSK+AISI +E AKRK
Sbjct: 361 VSVAAGILMHHAVCDRATRLVGCNGDLTSEEKQILLAEFSLRHSKSAISIVHEYAKRK 350

[Close]

BLAST of Lsi03G015200 vs. TrEMBL
Match: A0A0J8CYU2_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g039990 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.2e-72
Identity = 153/275 (55.64%), Postives = 181/275 (65.82%), Query Frame = 1


Query: 149 EALDPLILDSRKKRFKNVVQNRSYSVCLVVEG------LSDFGNVSAAFRSADALGFQSV 208
           + ++ L+    K +  N+++    SVC+          L  F        S + L   + 
Sbjct: 72  DTVEELLSPKNKDKVPNLMKMERKSVCMPDNHGRWFPYLDKFSANGVYLNSGEILEAVNP 131

Query: 209 HVVSCDASKRCN--NHGEFGSCFVSIVLAEFHTVLGAFTLLFNGSLGSNLLVECHCWFHM 268
            ++     K  N   H  +G C V   L++F  V  A          S  +V C      
Sbjct: 132 LILDVRKEKFRNVVKHRSYGVCLVVEGLSDFGNVSAALRSADALGFQSVHVVSCDS---- 191

Query: 269 VRRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCP 328
            +RYR+NRHVSMG+EKWLDIE+WNST ECF +LKSRGYRI STHLG N VSVYDMDWSCP
Sbjct: 192 SKRYRDNRHVSMGSEKWLDIEVWNSTRECFDVLKSRGYRIASTHLGKNTVSVYDMDWSCP 251

Query: 329 TAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHG 388
           TA+VVGNE++GIS+EALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLG HG
Sbjct: 252 TAVVVGNENKGISEEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGSHG 311

Query: 389 DLTSEERQILLAEFSLRHSKNAISIANELAKRKGS 416
           DLTS+E QILLAEF LRHSK+AIS A E A RK S
Sbjct: 312 DLTSDESQILLAEFFLRHSKSAISTACEYATRKSS 342

[Close]

BLAST of Lsi03G015200 vs. TAIR10
Match: AT5G15390.1 (AT5G15390.1 tRNA/rRNA methyltransferase (SpoU) family protein)

HSP 1 Score: 262.3 bits (669), Expect = 5.1e-70
Identity = 135/223 (60.54%), Postives = 161/223 (72.20%), Query Frame = 1


Query: 193 SADALGFQSVHVVS--CDASKRCNNHGEFGSCFVSIVLAEFHTVLGAFTLLFNGSLGSNL 252
           S + L   S H++    D  +R   +  +  C V   L++F  +  AF       + S  
Sbjct: 125 SREVLEAVSPHMMEERTDRFRRVVENRSYSVCLVVEGLSDFGNISAAFRSADALGIQSVH 184

Query: 253 LVECHCWFHMVRRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAV 312
           +V C       +RY  NRHVSMGAEKWLDIE W++  ECFK+LKSRGYRI +THLG + V
Sbjct: 185 VVSCDS----SKRYNGNRHVSMGAEKWLDIEFWDTPKECFKVLKSRGYRIATTHLGMDTV 244

Query: 313 SVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVC 372
           S+YDMDWSCPTAIVVGNE RGISDEALELSDL CSIPMNGMVDSFNVSVAAGILMHHAV 
Sbjct: 245 SIYDMDWSCPTAIVVGNEGRGISDEALELSDLRCSIPMNGMVDSFNVSVAAGILMHHAVS 304

Query: 373 DRTSRLGCHGDLTSEERQILLAEFSLRHSKNAISIANELAKRK 414
           DRT+RLG HGDL+  E++IL+AEFSLRHS+++I IA E AKRK
Sbjct: 305 DRTTRLGSHGDLSEAEKEILMAEFSLRHSRSSICIAYEFAKRK 343

[Close]

BLAST of Lsi03G015200 vs. NCBI nr
Match: gi|659085333|ref|XP_008443364.1| (PREDICTED: uncharacterized protein LOC103486969 [Cucumis melo])

HSP 1 Score: 451.8 bits (1161), Expect = 1.3e-123
Identity = 270/428 (63.08%), Postives = 305/428 (71.26%), Query Frame = 1


Query: 1   MASYRIVARSSLILLESCSPRRPQRIFPSVSLPSLPLPSSSPFFTIVTNPLSRSSPNAVS 60
           MAS RIV+RSSL LL+S SPRR QR+FPSV LPSL LPSS PFFT   N LSRS+PNAVS
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPSS-PFFT---NTLSRSNPNAVS 60

Query: 61  TRCLASISAATSSIEVADTVGDLEPPEIHTQDTVEHLLTHADDVTRLMKMERRPLELDGA 120
           +RC ASISAATS +E+AD V D+EPPE+ TQDTVEHLLTH+DDV+RLMKMERRP+ELDGA
Sbjct: 61  SRCFASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGA 120

Query: 121 EYPHKGRRFPYLDKFSCGSSWLNSGEILEALDPLILDSRKKRFKNVVQNRSYSVCLVVEG 180
           EYPHKGR FPYLDK+S GSSWLNSGEILEALD LILD+RK+RFKNVV+NRSYSVCLVVEG
Sbjct: 121 EYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEG 180

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRC--NNHGEFGS-CFVSIVLAEFHTVLGA 240
           LSDFGNVSAAFRSADALGFQSVHVVSCDASKR   N H   G+  ++ I L  +++    
Sbjct: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIEL--WNSTSEC 240

Query: 241 FTLLFNGSLGSNLLVECHCWFHMVRRYR-ENRHVSMGAEKWLDIELWNSTPECFKILKSR 300
           F +L +                  R YR  + H+   A    D++ W+         +SR
Sbjct: 241 FKILKS------------------RGYRIVSTHLGANAVSVYDMD-WSCPTAIVVGNESR 300

Query: 301 GYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFN 360
           G       +   A+ + D+  S P                            NGMVDSFN
Sbjct: 301 G-------ISNEALELSDLHCSIPM---------------------------NGMVDSFN 360

Query: 361 VSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKNAISIANELAKRKGSV 420
           VSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSK+AISIANELAKRKGS 
Sbjct: 361 VSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSA 369

Query: 421 ISGTDVLL 425
           IS T VLL
Sbjct: 421 ISSTGVLL 369

[Close]

BLAST of Lsi03G015200 vs. NCBI nr
Match: gi|700204397|gb|KGN59530.1| (hypothetical protein Csa_3G824750 [Cucumis sativus])

HSP 1 Score: 450.7 bits (1158), Expect = 2.9e-123
Identity = 268/428 (62.62%), Postives = 302/428 (70.56%), Query Frame = 1


Query: 1   MASYRIVARSSLILLESCSPRRPQRIFPSVSLPSLPLPSSSPFFTIVTNPLSRSSPNAVS 60
           MAS RIV+RSSL LL+SCSPRR QRIF SV LPSL LPS S FFT   N  SRS+PNAVS
Sbjct: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT---NRFSRSNPNAVS 60

Query: 61  TRCLASISAATSSIEVADTVGDLEPPEIHTQDTVEHLLTHADDVTRLMKMERRPLELDGA 120
           TRC ASISAATS +EV D V D+EPPE+ TQDTVEHLLTH++DV RLMKMERR +ELDGA
Sbjct: 61  TRCFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGA 120

Query: 121 EYPHKGRRFPYLDKFSCGSSWLNSGEILEALDPLILDSRKKRFKNVVQNRSYSVCLVVEG 180
           EYPHKGR FPYLDK+SCGSSWLNSGEILEALD LILDSRK+RFKNVV+NRSYSVCLVVEG
Sbjct: 121 EYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEG 180

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRC--NNHGEFGS-CFVSIVLAEFHTVLGA 240
           L+DFGNVSAAFRSADALGFQSVHVVSCDASKR   N H   G+  ++ I L  +++    
Sbjct: 181 LTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIEL--WNSTPEC 240

Query: 241 FTLLFNGSLGSNLLVECHCWFHMVRRYR-ENRHVSMGAEKWLDIELWNSTPECFKILKSR 300
           F +L +                  R YR  + H+   A    D++ W+         +SR
Sbjct: 241 FKILKS------------------RGYRIVSTHLGANAVSVYDMD-WSCPTAIVVGNESR 300

Query: 301 GYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFN 360
           G       +   A+ + D+  S P                            NGMVDSFN
Sbjct: 301 G-------ISNEALELSDLHCSIPM---------------------------NGMVDSFN 360

Query: 361 VSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKNAISIANELAKRKGSV 420
           VSVAAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSK+AISIANELAKRKGS 
Sbjct: 361 VSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSA 370

Query: 421 ISGTDVLL 425
           IS T+VLL
Sbjct: 421 ISSTEVLL 370

[Close]

BLAST of Lsi03G015200 vs. NCBI nr
Match: gi|778685436|ref|XP_004136796.2| (PREDICTED: uncharacterized protein LOC101218911 isoform X1 [Cucumis sativus])

HSP 1 Score: 450.7 bits (1158), Expect = 2.9e-123
Identity = 268/428 (62.62%), Postives = 302/428 (70.56%), Query Frame = 1


Query: 1   MASYRIVARSSLILLESCSPRRPQRIFPSVSLPSLPLPSSSPFFTIVTNPLSRSSPNAVS 60
           MAS RIV+RSSL LL+SCSPRR QRIF SV LPSL LPS S FFT   N  SRS+PNAVS
Sbjct: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT---NRFSRSNPNAVS 103

Query: 61  TRCLASISAATSSIEVADTVGDLEPPEIHTQDTVEHLLTHADDVTRLMKMERRPLELDGA 120
           TRC ASISAATS +EV D V D+EPPE+ TQDTVEHLLTH++DV RLMKMERR +ELDGA
Sbjct: 104 TRCFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGA 163

Query: 121 EYPHKGRRFPYLDKFSCGSSWLNSGEILEALDPLILDSRKKRFKNVVQNRSYSVCLVVEG 180
           EYPHKGR FPYLDK+SCGSSWLNSGEILEALD LILDSRK+RFKNVV+NRSYSVCLVVEG
Sbjct: 164 EYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEG 223

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRC--NNHGEFGS-CFVSIVLAEFHTVLGA 240
           L+DFGNVSAAFRSADALGFQSVHVVSCDASKR   N H   G+  ++ I L  +++    
Sbjct: 224 LTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIEL--WNSTPEC 283

Query: 241 FTLLFNGSLGSNLLVECHCWFHMVRRYR-ENRHVSMGAEKWLDIELWNSTPECFKILKSR 300
           F +L +                  R YR  + H+   A    D++ W+         +SR
Sbjct: 284 FKILKS------------------RGYRIVSTHLGANAVSVYDMD-WSCPTAIVVGNESR 343

Query: 301 GYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFN 360
           G       +   A+ + D+  S P                            NGMVDSFN
Sbjct: 344 G-------ISNEALELSDLHCSIPM---------------------------NGMVDSFN 403

Query: 361 VSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKNAISIANELAKRKGSV 420
           VSVAAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSK+AISIANELAKRKGS 
Sbjct: 404 VSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSA 413

Query: 421 ISGTDVLL 425
           IS T+VLL
Sbjct: 464 ISSTEVLL 413

[Close]

BLAST of Lsi03G015200 vs. NCBI nr
Match: gi|778685447|ref|XP_011652219.1| (PREDICTED: uncharacterized protein LOC101218911 isoform X2 [Cucumis sativus])

HSP 1 Score: 435.3 bits (1118), Expect = 1.3e-118
Identity = 261/428 (60.98%), Postives = 294/428 (68.69%), Query Frame = 1


Query: 1   MASYRIVARSSLILLESCSPRRPQRIFPSVSLPSLPLPSSSPFFTIVTNPLSRSSPNAVS 60
           MAS RIV+RSSL LL+SCSPRR QRIF SV LPSL LPS S FFT           N  S
Sbjct: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT-----------NRFS 103

Query: 61  TRCLASISAATSSIEVADTVGDLEPPEIHTQDTVEHLLTHADDVTRLMKMERRPLELDGA 120
           TRC ASISAATS +EV D V D+EPPE+ TQDTVEHLLTH++DV RLMKMERR +ELDGA
Sbjct: 104 TRCFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGA 163

Query: 121 EYPHKGRRFPYLDKFSCGSSWLNSGEILEALDPLILDSRKKRFKNVVQNRSYSVCLVVEG 180
           EYPHKGR FPYLDK+SCGSSWLNSGEILEALD LILDSRK+RFKNVV+NRSYSVCLVVEG
Sbjct: 164 EYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEG 223

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRC--NNHGEFGS-CFVSIVLAEFHTVLGA 240
           L+DFGNVSAAFRSADALGFQSVHVVSCDASKR   N H   G+  ++ I L  +++    
Sbjct: 224 LTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIEL--WNSTPEC 283

Query: 241 FTLLFNGSLGSNLLVECHCWFHMVRRYR-ENRHVSMGAEKWLDIELWNSTPECFKILKSR 300
           F +L +                  R YR  + H+   A    D++ W+         +SR
Sbjct: 284 FKILKS------------------RGYRIVSTHLGANAVSVYDMD-WSCPTAIVVGNESR 343

Query: 301 GYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFN 360
           G       +   A+ + D+  S P                            NGMVDSFN
Sbjct: 344 G-------ISNEALELSDLHCSIPM---------------------------NGMVDSFN 403

Query: 361 VSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKNAISIANELAKRKGSV 420
           VSVAAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSK+AISIANELAKRKGS 
Sbjct: 404 VSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSA 405

Query: 421 ISGTDVLL 425
           IS T+VLL
Sbjct: 464 ISSTEVLL 405

[Close]

BLAST of Lsi03G015200 vs. NCBI nr
Match: gi|720007054|ref|XP_010258185.1| (PREDICTED: uncharacterized protein LOC104598020 isoform X1 [Nelumbo nucifera])

HSP 1 Score: 320.5 bits (820), Expect = 4.5e-84
Identity = 216/423 (51.06%), Postives = 257/423 (60.76%), Query Frame = 1


Query: 9   RSSLILLESCSPRRPQRIFPSVSLPSLPLPSSSPFFTIVTNPLSRSSP-----NAVSTRC 68
           RSS+  LE C+ R  +R   SV L S+   +S+ +FT   +PLSR+       +   +RC
Sbjct: 6   RSSVSSLE-CNLRSNRRELSSVFLRSVYSNASNLWFT---SPLSRNRGLGFRYSRAHSRC 65

Query: 69  LASISAATSSIEVADTVGD-------LEPPEIHTQDTVEHLLTHADDVTRLMKMERRPLE 128
              +  A S  E  D + +       LEPP   T+D VE LL + D V RLMKMERR   
Sbjct: 66  YGCVQGAISR-ETEDCLDEAASGNLILEPPCNLTKDNVEELLGNRDSVARLMKMERRSEV 125

Query: 129 LDGAEYPHKGRRFPYLDKFSCGSSWLNSGEILEALDPLILDSRKKRFKNVVQNRSYSVCL 188
            DG E+  KGR FPYLD F  GS++L S E+LEA+DP ILD RK+R + VV+NRSYSVCL
Sbjct: 126 EDGVEFSRKGRWFPYLDMFKTGSTFLTSREVLEAVDPYILDVRKERIRKVVKNRSYSVCL 185

Query: 189 VVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNHGEFGSCFVSIVLAEFHTVLG 248
           VVEGL+DFGNVSA FRSADALGFQSVHVVSCD+SKR  ++               H  +G
Sbjct: 186 VVEGLTDFGNVSAVFRSADALGFQSVHVVSCDSSKRYRDNR--------------HVSMG 245

Query: 249 AFTLLFNGSLGSNLLVECHCWFHMV--RRYR-ENRHVSMGAEKW---LDIELWNSTPECF 308
           A   L      S    EC   F ++  R YR    HV + A  W   + + +++    C 
Sbjct: 246 AEKWLDIELWNST--KEC---FRVLKSRGYRIATTHVGIDA-VWIGLISVSIYDMDWSCP 305

Query: 309 KILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 368
                                         TAIVVGNESRGISDEAL+LSDLHCSIPM G
Sbjct: 306 ------------------------------TAIVVGNESRGISDEALKLSDLHCSIPMKG 365

Query: 369 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKNAISIANELA 414
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDL  EE QILLAEFSLRHSKNAI+IA+E A
Sbjct: 366 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLPIEESQILLAEFSLRHSKNAINIAHEYA 373

The following BLAST results are available for this feature:

BLAST of Lsi03G015200 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TRMH_AQUAE	1.6e-17	34.81	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) GN=...	[more]
TRMH_SHIFL	1.8e-16	34.19	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri GN=trmH PE=3 SV...	[more]
TRMH_ECOLI	1.8e-16	34.19	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) GN=...	[more]
TRMH_ECO57	1.8e-16	34.19	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 GN=trmH ...	[more]
TRMH_THET8	1.7e-14	40.82	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain HB8 ...	[more]

BLAST of Lsi03G015200 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LC15_CUCSA	2.0e-123	62.62	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G824750 PE=4 SV=1	[more]
M5WSN7_PRUPE	1.3e-74	68.75	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008118mg PE=4 SV=1	[more]
A0A067FZF2_CITSI	5.0e-74	46.76	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g018308mg PE=4 SV=1	[more]
A0A067FZB2_CITSI	1.2e-72	46.65	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g018308mg PE=4 SV=1	[more]
A0A0J8CYU2_BETVU	1.2e-72	55.64	Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g039990 PE=4 S...	[more]

BLAST of Lsi03G015200 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G15390.1	5.1e-70	60.54	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

BLAST of Lsi03G015200 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659085333\|ref\|XP_008443364.1\|	1.3e-123	63.08	PREDICTED: uncharacterized protein LOC103486969 [Cucumis melo]	[more]
gi\|700204397\|gb\|KGN59530.1\|	2.9e-123	62.62	hypothetical protein Csa_3G824750 [Cucumis sativus]	[more]
gi\|778685436\|ref\|XP_004136796.2\|	2.9e-123	62.62	PREDICTED: uncharacterized protein LOC101218911 isoform X1 [Cucumis sativus]	[more]
gi\|778685447\|ref\|XP_011652219.1\|	1.3e-118	60.98	PREDICTED: uncharacterized protein LOC101218911 isoform X2 [Cucumis sativus]	[more]
gi\|720007054\|ref\|XP_010258185.1\|	4.5e-84	51.06	PREDICTED: uncharacterized protein LOC104598020 isoform X1 [Nelumbo nucifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008173	RNA methyltransferase activity
GO:0003723	RNA binding
GO:0008168	methyltransferase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006396	RNA processing
GO:0030488	tRNA methylation

Vocabulary: **INTERPRO**
Term	Definition
IPR001537	SpoU_MeTrfase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009451	RNA modification
biological_process	GO:0006396	RNA processing
biological_process	GO:0001510	RNA methylation
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0009020	tRNA (guanosine-2'-O-)-methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G015200.1	Lsi03G015200.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 265..366 score: 1.3
None	No IPR available	PANTHER	PTHR12029	RNA METHYLTRANSFERASE	coord: 87..216 score: 3.7E-154coord: 268..393 score: 3.7E
None	No IPR available	PANTHER	PTHR12029:SF45	RRNA METHYLASE-LIKE PROTEIN	coord: 87..216 score: 3.7E-154coord: 268..393 score: 3.7E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G015200	Csa3G824750	Cucumber (Chinese Long) v2	culsiB186
Lsi03G015200	CSPI03G38640	Wild cucumber (PI 183967)	cpilsiB184
Lsi03G015200	Cucsa.338500	Cucumber (Gy14) v1	cgylsiB551
Lsi03G015200	CmaCh11G011530	Cucurbita maxima (Rimu)	cmalsiB114
Lsi03G015200	CmoCh11G011950	Cucurbita moschata (Rifu)	cmolsiB106
Lsi03G015200	Cla016971	Watermelon (97103) v1	lsiwmB240
Lsi03G015200	MELO3C009837	Melon (DHL92) v3.5.1	lsimeB260
Lsi03G015200	ClCG10G015450	Watermelon (Charleston Gray)	lsiwcgB219
Lsi03G015200	MELO3C009837.2	Melon (DHL92) v3.6.1	lsimedB273
Lsi03G015200	Cla97C10G198300	Watermelon (97103) v2	lsiwmbB218
Lsi03G015200	Bhi11G002445	Wax gourd	lsiwgoB300
Lsi03G015200	Cp4.1LG04g00370	Cucurbita pepo (Zucchini)	cpelsiB546
Lsi03G015200	CsGy3G036170	Cucumber (Gy14) v2	cgyblsiB171
Lsi03G015200	Carg25013	Silver-seed gourd	carlsiB220
Lsi03G015200	CsaV3_3G041040	Cucumber (Chinese Long) v3	cuclsiB203

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G015200	Wax gourd	lsiwgoB313
Lsi03G015200	Cucumber (Gy14) v1	cgylsiB255
Lsi03G015200	Cucurbita maxima (Rimu)	cmalsiB242
Lsi03G015200	Cucurbita maxima (Rimu)	cmalsiB658
Lsi03G015200	Cucurbita moschata (Rifu)	cmolsiB230
Lsi03G015200	Cucurbita moschata (Rifu)	cmolsiB647
Lsi03G015200	Cucurbita pepo (Zucchini)	cpelsiB145
Lsi03G015200	Cucurbita pepo (Zucchini)	cpelsiB503
Lsi03G015200	Wild cucumber (PI 183967)	cpilsiB359
Lsi03G015200	Cucumber (Chinese Long) v2	culsiB356
Lsi03G015200	Melon (DHL92) v3.6.1	lsimedB245
Lsi03G015200	Silver-seed gourd	carlsiB238
Lsi03G015200	Silver-seed gourd	carlsiB394
Lsi03G015200	Cucumber (Chinese Long) v3	cuclsiB381