Cucsa.338500 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.338500
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	tRNA guanosine-2'-O-methyltransferase
Location	scaffold03356 : 1365129 .. 1367719 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTGAACCTCTTTCAACGCACATCCATGGCGTCCTGCAGAATCGTATCCCGTTCTTCTCTTTTTCTGCTCCAAAGCTGCAGTCCCCGAAGAACTCAACGGATTTTCTCTTCTGTTTTTCTCCCTTCTCTACACCTTCCCTCCTTCTCTCTGTTCTTCACTAACAGATGTACTGTCTTCTCTCTTTCCCTCTCTTGGAGCCCTATCTTTACATTTCTTTTTATTAGTCTCTTCTTTACTTTATTTCTCTTTTTATTGCTACATAGTCTCAAGAAGCAACCCTAACGCAGTTTCTACAAGATGTTTCGCTTCAATTTCCGCCGCAACATCGCCACTAGAAGTAGAAGATATTGTGGCTGACGTAGAACCACCGGAGGTTCGTACACAAGACACGGTGGAGCACCTGCTCACGCATTCCAATGACGTTGCTAGGTTGATGAAGATGGAACGGAGGTCGGTTGAGTTAGATGGAGCTGAATATCCACATAAAGGGCGGTGGTTTCCGTATCTGGATAAGTATAGCTGTGGAAGTTCTTGGTTGAATAGTGGTGAGATTTTGGAGGCCTTGGATTCTCTCATATTGGACTCGAGGAAAGAGCGGTTCAAGAACGTGGTGCGGAATAGAAGCTACTCCGTTTGTTTGGTGGTGGAGGGATTGACAGATTTCGGTAACGTCTCGGCGGCGTTTCGATCTGCTGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAACAACCATGGAGGTGAAATTGTTTTCGAATGCATTTGTTTTTTTTTTtAATTTCTTTCGGGAAAATTGTTTGATTCTGGAATTTGGAAGCTGGTTTGTTTTCAATGTGATGGTGTGGAATTTCATTACTGTTCTTGTGCTTTTACTTTGTTGTTCAATGGACCATTTGAATTCAATCAATTAGTCGAATGCCCTTGCTGGTTAATATGGTTAGAAGGTTAAACCGATCAAATACGTTTGATTTTGATTGGTAATTTTTAAATACGGATGACAATGTGACACAGATTGAGCAATGGAATTCCTGCAAATGTGCATCTCTTGTATATATTGTATCTTTATATGCACATAACCATTGAAAACTTTATACCAAATGTCGATTTTAATGTATTATTTTTCATTGCACAACGTTTATTTTCCATGTGAAAGTATATGTTCATATGTTTTTTCTTCCTTCATTTGCCATCTTTATCTCGCCATTTCATAACATTAAGTCATATTTGTCTTGGGCATGAAACCTTTTCCAGTGCTTGTATTCTCATGCTTACCCTGAATGAGATCATGCTTTGATAAATTAGGGTGCAACATGAGTTCGTTCAGCTTACTAAGAATGGCAAAAATTCCCATTGCTTACATTTTCTTTGTGAATATTTTCTCGTATTTCTTCATCCGACATCATTGCTATTCAATTCAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCTACCCCAGAATGTTTTAAAATTCTGAAATCCCGGGGTTATAGAATAGTCTCTACTCATCTAGGAGCGAATGCGGTATTGATTGATTTTGTAAGCCCTTCTTTCACATTAGAAATTTTGTAAGCTTGTCCTGACAGCTGAATTGCGATTGTCCTGTTGTAGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTGGGGAATGAAAGTAGGTAATTATCTTCTTGCTCCAGTAATATGGAGTGTGATCAAGTTCAAAGCAATTAGTTACGGGATGATTGCAATAGCTGTTTTCTCTATGTTATTCGATGAACATTTTTGAATCACTGCAAGTTGGGAGATAATAGATATCAGTAAAATTTAGGAAATCATAACTGAATACACGCTTAAAAGTAGCAACATTTTTTTGAAATTGACAATTATCTCATGCTTCAGTTAGCAAGTTTCGACGCATGAATGATGTCGGAGTCTGGAGAAACTATACTTGTAGCTATGTTGATTTCTGAGGTTAAGATTTCTTCCTTTTTTAGGGGAATAAGTAATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTTTCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCAATCGGACTTCTCGGCTGGTAACTATAAAAAACCATGTTTGAATACTCTTGGATTCATCAGGCCATAAGGGTTTGGATAGCTCAATAAGGGAGAATTCTCATCGTCTCTCTCAGAGCTGGAATTCGAAAATGCTTCTATCATAGAATTGATGGATTATGATGTTCTTTATCAAACCTTTCAGGGCTGTCATGGAGATCTTACATCAGAAGAAAGACAAATTCTACTGGCGGAGTTCTCTCTTAGGCATAGCAAGAGTGCAATCAGCATTGCGAACGAGTTGGCAAAGCGGAAGGGTTCCGCAATCTCTAGTACGGAGGTATTGCTGTAGAATGCCAAAAGTTTTGGGCATTTGATGTGTTGGAAATGTTTACGAGAAGCAGAAACACAGGGAATCTCTTTCAAAGTTTACACTAAAACGGG

mRNA sequence

GTTGAACCTCTTTCAACGCACATCCATGGCGTCCTGCAGAATCGTATCCCGTTCTTCTCTTTTTCTGCTCCAAAGCTGCAGTCCCCGAAGAACTCAACGGAttttctcttctgtttttctcccttctctacaccttccctccttctctctgttcttcactaacagatTCTCAAGAAGCAACCCTAACGCAGTTTCTACAAGATGTTTCGCTTCAATTTCCGCCGCAACATCGCCACTAGAAGTAGAAGATATTGTGGCTGACGTAGAACCACCGGAGGTTCGTACACAAGACACGGTGGAGCACCTGCTCACGCATTCCAATGACGTTGCTAGGTTGATGAAGATGGAACGGAGGTCGGTTGAGTTAGATGGAGCTGAATATCCACATAAAGGGCGGTGGTTTCCGTATCTGGATAAGTATAGCTGTGGAAGTTCTTGGTTGAATAGTGGTGAGATTTTGGAGGCCTTGGATTCTCTCATATTGGACTCGAGGAAAGAGCGGTTCAAGAACGTGGTGCGGAATAGAAGCTACTCCGTTTGTTTGGTGGTGGAGGGATTGACAGATTTCGGTAACGTCTCGGCGGCGTTTCGATCTGCTGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCTACCCCAGAATGTTTTAAAATTCTGAAATCCCGGGGTTATAGAATAGTCTCTACTCATCTAGGAGCGAATGCGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTGGGGAATGAAAGTAGGGGAATAAGTAATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTTTCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCAATCGGACTTCTCGGCTGGGCTGTCATGGAGATCTTACATCAGAAGAAAGACAAATTCTACTGGCGGAGTTCTCTCTTAGGCATAGCAAGAGTGCAATCAGCATTGCGAACGAGTTGGCAAAGCGGAAGGGTTCCGCAATCTCTAGTACGGAGGTATTGCTGTAGAATGCCAAAAGTTTTGGGCATTTGATGTGTTGGAAATGTTTACGAGAAGCAGAAACACAGGGAATCTCTTTCAAAGTTTACACTAAAACGGG

Coding sequence (CDS)

ATGGCGTCCTGCAGAATCGTATCCCGTTCTTCTCTTTTTCTGCTCCAAAGCTGCAGTCCCCGAAGAACTCAACGGATTTTCTCTTCTGTTTTTCTCCCTTCTCTACACCTTCCCTCCTTCTCTCTGTTCTTCACTAACAGATTCTCAAGAAGCAACCCTAACGCAGTTTCTACAAGATGTTTCGCTTCAATTTCCGCCGCAACATCGCCACTAGAAGTAGAAGATATTGTGGCTGACGTAGAACCACCGGAGGTTCGTACACAAGACACGGTGGAGCACCTGCTCACGCATTCCAATGACGTTGCTAGGTTGATGAAGATGGAACGGAGGTCGGTTGAGTTAGATGGAGCTGAATATCCACATAAAGGGCGGTGGTTTCCGTATCTGGATAAGTATAGCTGTGGAAGTTCTTGGTTGAATAGTGGTGAGATTTTGGAGGCCTTGGATTCTCTCATATTGGACTCGAGGAAAGAGCGGTTCAAGAACGTGGTGCGGAATAGAAGCTACTCCGTTTGTTTGGTGGTGGAGGGATTGACAGATTTCGGTAACGTCTCGGCGGCGTTTCGATCTGCTGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCTACCCCAGAATGTTTTAAAATTCTGAAATCCCGGGGTTATAGAATAGTCTCTACTCATCTAGGAGCGAATGCGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTGGGGAATGAAAGTAGGGGAATAAGTAATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTTTCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCAATCGGACTTCTCGGCTGGGCTGTCATGGAGATCTTACATCAGAAGAAAGACAAATTCTACTGGCGGAGTTCTCTCTTAGGCATAGCAAGAGTGCAATCAGCATTGCGAACGAGTTGGCAAAGCGGAAGGGTTCCGCAATCTCTAGTACGGAGGTATTGCTGTAG

Protein sequence

MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRCFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSAISSTEVLL*

Homology

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BLAST of Cucsa.338500 vs. Swiss-Prot
Match: TRMH_AQUAE (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) GN=trmH PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.9e-22
Identity = 61/195 (31.28%), Postives = 113/195 (57.95%), Query Frame = 1


Query: 148 LDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDAS 207
           ++ L+L+ R +R + V+  R   + +  + + +  N SA  R+ DA+G   ++    +  
Sbjct: 3   MEYLVLEKRLKRLREVLEKRQKDLIVFADNVKNEHNFSAIVRTCDAVGVLYLYYYHAEG- 62

Query: 208 KRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPT 267
           K+ + N  ++ G+ KW+ IE  ++  +     K+RG++IV+T L   +V+  ++D++ PT
Sbjct: 63  KKAKINEGITQGSHKWVFIEKVDNPVQKLLEFKNRGFQIVATWLSKESVNFREVDYTKPT 122

Query: 268 AIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSR-LGCHG 327
            +VVGNE +G+S E +E++D    IPM GM  S NVSVA GI+++ A   R  + +    
Sbjct: 123 VLVVGNELQGVSPEIVEIADKKIVIPMYGMAQSLNVSVATGIILYEAQRQREEKGMYSRP 182

Query: 328 DLTSEERQILLAEFS 342
            L+ EE Q +L +++
Sbjct: 183 SLSEEEIQKILKKWA 196

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BLAST of Cucsa.338500 vs. Swiss-Prot
Match: TRMH_SHIFL (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri GN=trmH PE=3 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.1e-20
Identity = 69/209 (33.01%), Postives = 108/209 (51.67%), Query Frame = 1


Query: 155 SRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 214
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 215 HVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNE 274
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 275 SRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSR---LGCHGDLTSE 334
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 335 ERQILLAEFSLRHSKSAISIANELAKRKG 361
           E+Q LL E           +  ++AKRKG
Sbjct: 184 EQQRLLFE-------GGYPVLAKVAKRKG 203

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BLAST of Cucsa.338500 vs. Swiss-Prot
Match: TRMH_ECOLI (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) GN=trmH PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.1e-20
Identity = 69/209 (33.01%), Postives = 108/209 (51.67%), Query Frame = 1


Query: 155 SRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 214
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 215 HVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNE 274
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 275 SRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSR---LGCHGDLTSE 334
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 335 ERQILLAEFSLRHSKSAISIANELAKRKG 361
           E+Q LL E           +  ++AKRKG
Sbjct: 184 EQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of Cucsa.338500 vs. Swiss-Prot
Match: TRMH_ECO57 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 GN=trmH PE=3 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.1e-20
Identity = 69/209 (33.01%), Postives = 108/209 (51.67%), Query Frame = 1


Query: 155 SRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 214
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 215 HVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNE 274
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 275 SRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSR---LGCHGDLTSE 334
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 335 ERQILLAEFSLRHSKSAISIANELAKRKG 361
           E+Q LL E           +  ++AKRKG
Sbjct: 184 EQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of Cucsa.338500 vs. Swiss-Prot
Match: TRMH_THET2 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=trmH PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.4e-19
Identity = 56/162 (34.57%), Postives = 91/162 (56.17%), Query Frame = 1


Query: 154 DSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDAS-KRYRE 213
           ++R+ R + V+R R   + +++E +    N+SA  R+ DA+G    H V+       + E
Sbjct: 6   EARRRRIEEVLRRRQPDLTVLLENVHKPHNLSAILRTCDAVGVLEAHAVNPTGGVPTFNE 65

Query: 214 NRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVG 273
               S G+ KW+ + +     E F+ LK RG+ + +T L  +A    ++D++ PTA++ G
Sbjct: 66  T---SGGSHKWVYLRVHPDLHEAFRFLKERGFTVYATALREDARDFREVDYTKPTAVLFG 125

Query: 274 NESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHA 315
            E  G+S EAL L+D    IPM GMV S NVSVAA +++  A
Sbjct: 126 AEKWGVSEEALALADGAIKIPMLGMVQSLNVSVAAAVILFEA 164

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BLAST of Cucsa.338500 vs. TrEMBL
Match: A0A0A0LC15_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G824750 PE=4 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 1.2e-209
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 1


Query: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC 60
           MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC
Sbjct: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC 60

Query: 61  FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 120
           FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP
Sbjct: 61  FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 120

Query: 121 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 180
           HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD
Sbjct: 121 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 180

Query: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 240
           FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK
Sbjct: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 240

Query: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300
           SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS
Sbjct: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300

Query: 301 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360
           FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG
Sbjct: 301 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360

Query: 361 SAISSTEVLL 371
           SAISSTEVLL
Sbjct: 361 SAISSTEVLL 370

[Close]

BLAST of Cucsa.338500 vs. TrEMBL
Match: W9QIA5_9ROSA (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Morus notabilis GN=L484_020971 PE=4 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 6.5e-126
Identity = 237/362 (65.47%), Postives = 281/362 (77.62%), Query Frame = 1


Query: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHL-PSFSLFFTNRFSRSNPNAVSTR 60
           MA+C+ +   SL  + S        + S    PSL   PS S   + +  R+  N V +R
Sbjct: 1   MAACKTLVSISLSSVASTLRSSRLTLSSPHNSPSLCFSPSIS---SPQGLRALQNRVLSR 60

Query: 61  CFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEY 120
            +ASI  A+  +E  D   ++EPPE  TQD +EHLLTH +DV RLMKMERR ++ D    
Sbjct: 61  SYASIEGASLSIESIDSPEEIEPPESPTQDNLEHLLTHKDDVVRLMKMERRRIDTD---- 120

Query: 121 PHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLT 180
              GRWFPYLD++ CG ++L+SGE++EAL+  I++SR ERF+NV  NRSYSVCLVVEGL+
Sbjct: 121 QRTGRWFPYLDRFKCGDAYLSSGEVVEALEPHIMESRMERFRNVATNRSYSVCLVVEGLS 180

Query: 181 DFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKIL 240
           DFGNVSAAFRSADALGFQSVHVVSCD SKRYRENRHVS GAEKWLDIE+W+S  +CFK L
Sbjct: 181 DFGNVSAAFRSADALGFQSVHVVSCDGSKRYRENRHVSKGAEKWLDIEVWDSIEDCFKAL 240

Query: 241 KSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVD 300
           KSRGYRI +TH+G + VS+Y+MDWSCPTAIVVGNE+RGIS+EAL LSDLHCSIPM GMVD
Sbjct: 241 KSRGYRIATTHVGMDVVSIYEMDWSCPTAIVVGNENRGISDEALGLSDLHCSIPMKGMVD 300

Query: 301 SFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRK 360
           SFNVSVAAGILMHHAVC+R SRLG HGDLT EERQILLAEFSLRH KS+ISIA+E AKRK
Sbjct: 301 SFNVSVAAGILMHHAVCDRISRLGSHGDLTVEERQILLAEFSLRHGKSSISIAHEFAKRK 355

Query: 361 GS 362
            +
Sbjct: 361 AA 355

[Close]

BLAST of Cucsa.338500 vs. TrEMBL
Match: M5WSN7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008118mg PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.1e-125
Identity = 243/366 (66.39%), Postives = 283/366 (77.32%), Query Frame = 1


Query: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRS-NPNAVSTR 60
           M++C+ + R+SL  + S          S   +P LH PS       RFS   +PN     
Sbjct: 1   MSACKTLVRTSLSSISSSLRA------SGPPIPLLHSPSL------RFSPLLSPNQ---- 60

Query: 61  CFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEY 120
                 AA    E +   + +EPPE+ TQDTVE LL   NDV RLMKMER       + +
Sbjct: 61  -----EAADFTDEAQFTDSTLEPPEIPTQDTVEQLLKQKNDVGRLMKMERSPE----SAH 120

Query: 121 PHKGRWFPYLDKYSCGS-SWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGL 180
              GRWFPYLDK+SCG  ++LNS E+L+ALD  ILD RKERF+ VV+NRSYSVCLVVEGL
Sbjct: 121 QRGGRWFPYLDKFSCGEDAYLNSAEVLDALDPYILDVRKERFRRVVKNRSYSVCLVVEGL 180

Query: 181 TDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKI 240
            DFGNVSAAFRSADALGFQSVHV+SCD+SKRYR+NRHVSMGAEKWLDIELW+STPECF++
Sbjct: 181 GDFGNVSAAFRSADALGFQSVHVLSCDSSKRYRDNRHVSMGAEKWLDIELWDSTPECFEV 240

Query: 241 LKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMV 300
           LKSRGYRI +TH+G +AVSVYDMDWSCPTAIVVGNE+RGIS+EAL+LSDLHCSIPM GMV
Sbjct: 241 LKSRGYRIATTHVGMDAVSVYDMDWSCPTAIVVGNENRGISDEALDLSDLHCSIPMKGMV 300

Query: 301 DSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKR 360
           DSFNVSVAAGILMHHAVC+RTSRLGCHGDLT EE QILLAEFSLRHSKS++SIA+E AKR
Sbjct: 301 DSFNVSVAAGILMHHAVCDRTSRLGCHGDLTFEESQILLAEFSLRHSKSSMSIAHEYAKR 341

Query: 361 KGSAIS 365
           K + ++
Sbjct: 361 KAAVLT 341

[Close]

BLAST of Cucsa.338500 vs. TrEMBL
Match: A0A059AR42_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I01991 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.7e-124
Identity = 243/362 (67.13%), Postives = 277/362 (76.52%), Query Frame = 1


Query: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHL---PSFSLFFTNRFSRSNPNAVS 60
           M+SCR + R+SLF L S   R + R   S+ L SLH    PS  L          P   +
Sbjct: 1   MSSCRTLVRASLFSLDS-GRRSSHRASFSLPLRSLHFLSPPSRGLGLLRA-----PGGAT 60

Query: 61  TRCFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGA 120
            R   SI  A + LE  +   +  P E   +D VE LL    DV +LMKMERR    D  
Sbjct: 61  ARRLGSIPGAVA-LEPTEASDEDSPAEGPAKDAVERLLIDREDVTQLMKMERRPAVGDPG 120

Query: 121 EYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEG 180
                GRWFPYLD++ CG+S L+SGE+LEALD LI+D RKERF++ VRNRSYSVCLVVEG
Sbjct: 121 -----GRWFPYLDRFRCGASHLSSGEVLEALDPLIMDVRKERFRSAVRNRSYSVCLVVEG 180

Query: 181 LTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFK 240
           L DFGNVSAAFRSADALGFQSVHVVSCD+SKRYRENRHVSMGAEKWLDIELW+ST ECF+
Sbjct: 181 LCDFGNVSAAFRSADALGFQSVHVVSCDSSKRYRENRHVSMGAEKWLDIELWDSTQECFR 240

Query: 241 ILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGM 300
           +L+SRGYRI +THL  +AVS+YDMDWS PTAIVVGNE+RGIS+EAL LSDLHCSIPM GM
Sbjct: 241 VLRSRGYRIATTHLATDAVSIYDMDWSYPTAIVVGNENRGISDEALRLSDLHCSIPMKGM 300

Query: 301 VDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAK 360
           VDSFNVSVAAGILMHHAVC+RTSRLGCHGDLT+EE QILLAEFSLRHSKSAISIA+E A+
Sbjct: 301 VDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTTEESQILLAEFSLRHSKSAISIAHEYAR 350

[Close]

BLAST of Cucsa.338500 vs. TrEMBL
Match: D7SXH6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0108g01380 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 3.3e-122
Identity = 239/371 (64.42%), Postives = 287/371 (77.36%), Query Frame = 1


Query: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTN-RFSRSNPNAVSTR 60
           M++C+ + R S+  L+  S    QR FS  F+P  + PSFS   ++ RF+    + +S  
Sbjct: 1   MSACKTLVRISISSLEF-SLVCNQRNFS--FIPLPNHPSFSPISSSLRFA----SLLSPA 60

Query: 61  CFASISAATSPLEVEDIVADVEPPEVRT--------QDTVEHLLTHSNDVARLMKMERRS 120
              S+      L     V   E   V T        +D VEHLLT+++DV RLMKMERRS
Sbjct: 61  SSISLGLTRHKLHSRPYVHVAESVGVETTDLEDDSNKDIVEHLLTNTDDVGRLMKMERRS 120

Query: 121 VELDGAEYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSV 180
           +  DG E     RWFPYLDK+  G+++L+S EILEA+D  I++ RKERF+NVV++RSYSV
Sbjct: 121 MTGDGGELIQSKRWFPYLDKFKTGTTFLSSSEILEAMDPYIMEVRKERFRNVVKSRSYSV 180

Query: 181 CLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNS 240
           CLVVEGL DFGNVSAAFRSADALGFQSVHVVSCD+SKRY++NRHVSMGAEKWLDIELWNS
Sbjct: 181 CLVVEGLCDFGNVSAAFRSADALGFQSVHVVSCDSSKRYKDNRHVSMGAEKWLDIELWNS 240

Query: 241 TPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCS 300
           T ECF++LKSRGYRI +TH+G +AVS+YDMDW+CPTAIVVGNE+RGIS+EALELSDLHCS
Sbjct: 241 TQECFEVLKSRGYRIATTHVGMDAVSIYDMDWTCPTAIVVGNENRGISDEALELSDLHCS 300

Query: 301 IPMNGMVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISI 360
           IPM GMVDSFNVSVAAGILMHHAVC+RTSRLG HGDL+ EE +ILLAEFSLRHSKSA SI
Sbjct: 301 IPMKGMVDSFNVSVAAGILMHHAVCDRTSRLGSHGDLSLEESRILLAEFSLRHSKSANSI 360

Query: 361 ANELAKRKGSA 363
           A+E AKRK ++
Sbjct: 361 AHEFAKRKAAS 364

[Close]

BLAST of Cucsa.338500 vs. TAIR10
Match: AT5G15390.1 (AT5G15390.1 tRNA/rRNA methyltransferase (SpoU) family protein)

HSP 1 Score: 390.6 bits (1002), Expect = 1.1e-108
Identity = 213/354 (60.17%), Postives = 258/354 (72.88%), Query Frame = 1


Query: 15  LQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTN-----RFSRSNPNA---VSTRCFASISA 74
           + SC       I S  F+P L  P+FS+ F +       S  +PN+   V+ R     + 
Sbjct: 1   MNSCKSLIRASISSFPFVP-LPNPNFSITFISVRAFSPLSVLHPNSSCIVTARRTFHGAV 60

Query: 75  ATSPLEVEDIVADVEPPEVRTQDTVEHLLT-HSNDVARLMKMERRSVELDGAEYPHKGRW 134
           A SP  + +     E P+    DTV+ LLT +  + + LMKMERR   L   E   +G W
Sbjct: 61  ALSPESLTE-----ESPK----DTVKGLLTTNRGEASSLMKMERRC-SLSNGEGDCRGSW 120

Query: 135 FPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVS 194
           FPY D++ CG   L+S E+LEA+   +++ R +RF+ VV NRSYSVCLVVEGL+DFGN+S
Sbjct: 121 FPYEDRFRCGEVHLSSREVLEAVSPHMMEERTDRFRRVVENRSYSVCLVVEGLSDFGNIS 180

Query: 195 AAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYR 254
           AAFRSADALG QSVHVVSCD+SKRY  NRHVSMGAEKWLDIE W++  ECFK+LKSRGYR
Sbjct: 181 AAFRSADALGIQSVHVVSCDSSKRYNGNRHVSMGAEKWLDIEFWDTPKECFKVLKSRGYR 240

Query: 255 IVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSV 314
           I +THLG + VS+YDMDWSCPTAIVVGNE RGIS+EALELSDL CSIPMNGMVDSFNVSV
Sbjct: 241 IATTHLGMDTVSIYDMDWSCPTAIVVGNEGRGISDEALELSDLRCSIPMNGMVDSFNVSV 300

Query: 315 AAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRK 360
           AAGILMHHAV +RT+RLG HGDL+  E++IL+AEFSLRHS+S+I IA E AKRK
Sbjct: 301 AAGILMHHAVSDRTTRLGSHGDLSEAEKEILMAEFSLRHSRSSICIAYEFAKRK 343

[Close]

BLAST of Cucsa.338500 vs. NCBI nr
Match: gi|700204397|gb|KGN59530.1| (hypothetical protein Csa_3G824750 [Cucumis sativus])

HSP 1 Score: 736.9 bits (1901), Expect = 1.8e-209
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 1


Query: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC 60
           MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC
Sbjct: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC 60

Query: 61  FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 120
           FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP
Sbjct: 61  FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 120

Query: 121 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 180
           HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD
Sbjct: 121 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 180

Query: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 240
           FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK
Sbjct: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 240

Query: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300
           SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS
Sbjct: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300

Query: 301 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360
           FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG
Sbjct: 301 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360

Query: 361 SAISSTEVLL 371
           SAISSTEVLL
Sbjct: 361 SAISSTEVLL 370

[Close]

BLAST of Cucsa.338500 vs. NCBI nr
Match: gi|778685436|ref|XP_004136796.2| (PREDICTED: uncharacterized protein LOC101218911 isoform X1 [Cucumis sativus])

HSP 1 Score: 736.9 bits (1901), Expect = 1.8e-209
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 1


Query: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC 60
           MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC
Sbjct: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC 103

Query: 61  FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 120
           FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP
Sbjct: 104 FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 163

Query: 121 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 180
           HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD
Sbjct: 164 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 223

Query: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 240
           FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK
Sbjct: 224 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 283

Query: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300
           SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS
Sbjct: 284 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 343

Query: 301 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360
           FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG
Sbjct: 344 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 403

Query: 361 SAISSTEVLL 371
           SAISSTEVLL
Sbjct: 404 SAISSTEVLL 413

[Close]

BLAST of Cucsa.338500 vs. NCBI nr
Match: gi|778685447|ref|XP_011652219.1| (PREDICTED: uncharacterized protein LOC101218911 isoform X2 [Cucumis sativus])

HSP 1 Score: 714.1 bits (1842), Expect = 1.2e-202
Identity = 362/370 (97.84%), Postives = 362/370 (97.84%), Query Frame = 1


Query: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC 60
           MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRF        STRC
Sbjct: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRF--------STRC 103

Query: 61  FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 120
           FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP
Sbjct: 104 FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 163

Query: 121 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 180
           HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD
Sbjct: 164 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 223

Query: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 240
           FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK
Sbjct: 224 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 283

Query: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300
           SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS
Sbjct: 284 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 343

Query: 301 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360
           FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG
Sbjct: 344 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 403

Query: 361 SAISSTEVLL 371
           SAISSTEVLL
Sbjct: 404 SAISSTEVLL 405

[Close]

BLAST of Cucsa.338500 vs. NCBI nr
Match: gi|659085333|ref|XP_008443364.1| (PREDICTED: uncharacterized protein LOC103486969 [Cucumis melo])

HSP 1 Score: 685.6 bits (1768), Expect = 4.7e-194
Identity = 349/370 (94.32%), Postives = 357/370 (96.49%), Query Frame = 1


Query: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC 60
           MASCRIVSRSSL LLQS SPRRTQR+F SVFLPSLHLPS S FFTN  SRSNPNAVS+RC
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFTNTLSRSNPNAVSSRC 60

Query: 61  FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 120
           FASISAATSPLE+ DIVADVEPPEV TQDTVEHLLTHS+DV+RLMKMERR VELDGAEYP
Sbjct: 61  FASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYP 120

Query: 121 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 180
           HKGRWFPYLDKYS GSSWLNSGEILEALDSLILD+RKERFKNVVRNRSYSVCLVVEGL+D
Sbjct: 121 HKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSD 180

Query: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 240
           FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNST ECFKILK
Sbjct: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTSECFKILK 240

Query: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300
           SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS
Sbjct: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300

Query: 301 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360
           FNVSVAAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG
Sbjct: 301 FNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360

Query: 361 SAISSTEVLL 371
           SAISST VLL
Sbjct: 361 SAISSTGVLL 369

[Close]

BLAST of Cucsa.338500 vs. NCBI nr
Match: gi|720007057|ref|XP_010258186.1| (PREDICTED: uncharacterized protein LOC104598020 isoform X2 [Nelumbo nucifera])

HSP 1 Score: 472.2 bits (1214), Expect = 8.1e-130
Identity = 247/371 (66.58%), Postives = 291/371 (78.44%), Query Frame = 1


Query: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNP-----NA 60
           M++C   S SSL     C+ R  +R  SSVFL S++  + +L+FT+  SR+       + 
Sbjct: 1   MSACLRSSVSSL----ECNLRSNRRELSSVFLRSVYSNASNLWFTSPLSRNRGLGFRYSR 60

Query: 61  VSTRCFASISAATSPLEVEDIVAD-------VEPPEVRTQDTVEHLLTHSNDVARLMKME 120
             +RC+  +  A S  E ED + +       +EPP   T+D VE LL + + VARLMKME
Sbjct: 61  AHSRCYGCVQGAISR-ETEDCLDEAASGNLILEPPCNLTKDNVEELLGNRDSVARLMKME 120

Query: 121 RRSVELDGAEYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRS 180
           RRS   DG E+  KGRWFPYLD +  GS++L S E+LEA+D  ILD RKER + VV+NRS
Sbjct: 121 RRSEVEDGVEFSRKGRWFPYLDMFKTGSTFLTSREVLEAVDPYILDVRKERIRKVVKNRS 180

Query: 181 YSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIEL 240
           YSVCLVVEGLTDFGNVSA FRSADALGFQSVHVVSCD+SKRYR+NRHVSMGAEKWLDIEL
Sbjct: 181 YSVCLVVEGLTDFGNVSAVFRSADALGFQSVHVVSCDSSKRYRDNRHVSMGAEKWLDIEL 240

Query: 241 WNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDL 300
           WNST ECF++LKSRGYRI +TH+G +AVS+YDMDWSCPTAIVVGNESRGIS+EAL+LSDL
Sbjct: 241 WNSTKECFRVLKSRGYRIATTHVGIDAVSIYDMDWSCPTAIVVGNESRGISDEALKLSDL 300

Query: 301 HCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSA 360
           HCSIPM GMVDSFNVSVAAGILMHHAVC+RTSRLGCHGDL  EE QILLAEFSLRHSK+A
Sbjct: 301 HCSIPMKGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLPIEESQILLAEFSLRHSKNA 360

The following BLAST results are available for this feature:

BLAST of Cucsa.338500 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TRMH_AQUAE	1.9e-22	31.28	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) GN=...	[more]
TRMH_SHIFL	1.1e-20	33.01	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri GN=trmH PE=3 SV...	[more]
TRMH_ECOLI	1.1e-20	33.01	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) GN=...	[more]
TRMH_ECO57	1.1e-20	33.01	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 GN=trmH ...	[more]
TRMH_THET2	3.4e-19	34.57	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain HB27...	[more]

BLAST of Cucsa.338500 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LC15_CUCSA	1.2e-209	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G824750 PE=4 SV=1	[more]
W9QIA5_9ROSA	6.5e-126	65.47	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Morus notabilis GN=L484_020971 PE...	[more]
M5WSN7_PRUPE	1.1e-125	66.39	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008118mg PE=4 SV=1	[more]
A0A059AR42_EUCGR	2.7e-124	67.13	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I01991 PE=4 SV=1	[more]
D7SXH6_VITVI	3.3e-122	64.42	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0108g01380 PE=4 SV=...	[more]

BLAST of Cucsa.338500 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G15390.1	1.1e-108	60.17	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

BLAST of Cucsa.338500 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700204397\|gb\|KGN59530.1\|	1.8e-209	100.00	hypothetical protein Csa_3G824750 [Cucumis sativus]	[more]
gi\|778685436\|ref\|XP_004136796.2\|	1.8e-209	100.00	PREDICTED: uncharacterized protein LOC101218911 isoform X1 [Cucumis sativus]	[more]
gi\|778685447\|ref\|XP_011652219.1\|	1.2e-202	97.84	PREDICTED: uncharacterized protein LOC101218911 isoform X2 [Cucumis sativus]	[more]
gi\|659085333\|ref\|XP_008443364.1\|	4.7e-194	94.32	PREDICTED: uncharacterized protein LOC103486969 [Cucumis melo]	[more]
gi\|720007057\|ref\|XP_010258186.1\|	8.1e-130	66.58	PREDICTED: uncharacterized protein LOC104598020 isoform X2 [Nelumbo nucifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001537	SpoU_MeTrfase

Vocabulary: **Molecular Function**
Term	Definition
GO:0008168	methyltransferase activity
GO:0003723	RNA binding
GO:0008173	RNA methyltransferase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0030488	tRNA methylation
GO:0006396	RNA processing

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001510	RNA methylation
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0006396	RNA processing
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.338500.1	Cucsa.338500.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 171..312 score: 9.8
None	No IPR available	PANTHER	PTHR12029	RNA METHYLTRANSFERASE	coord: 80..339 score: 8.4E
None	No IPR available	PANTHER	PTHR12029:SF45	RRNA METHYLASE-LIKE PROTEIN	coord: 80..339 score: 8.4E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.338500	Csa3G824750	Cucumber (Chinese Long) v2	cgycuB478
Cucsa.338500	MELO3C009837	Melon (DHL92) v3.5.1	cgymeB558
Cucsa.338500	CSPI03G38640	Wild cucumber (PI 183967)	cgycpiB504
Cucsa.338500	CmaCh11G011530	Cucurbita maxima (Rimu)	cgycmaB0924
Cucsa.338500	CmoCh11G011950	Cucurbita moschata (Rifu)	cgycmoB0924
Cucsa.338500	Lsi03G015200	Bottle gourd (USVL1VR-Ls)	cgylsiB551
Cucsa.338500	Cp4.1LG04g00370	Cucurbita pepo (Zucchini)	cgycpeB0895
Cucsa.338500	MELO3C009837.2	Melon (DHL92) v3.6.1	cgymedB559
Cucsa.338500	CsaV3_3G041040	Cucumber (Chinese Long) v3	cgycucB523
Cucsa.338500	Cla97C10G198300	Watermelon (97103) v2	cgywmbB582
Cucsa.338500	Bhi11G002445	Wax gourd	cgywgoB673

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.338500	Cucumber (Chinese Long) v3	cgycucB525
Cucsa.338500	Wax gourd	cgywgoB679
Cucsa.338500	Cucumber (Gy14) v1	cgycgyB098
Cucsa.338500	Cucurbita maxima (Rimu)	cgycmaB0926
Cucsa.338500	Cucurbita maxima (Rimu)	cgycmaB0932
Cucsa.338500	Cucurbita maxima (Rimu)	cgycmaB0939
Cucsa.338500	Cucurbita moschata (Rifu)	cgycmoB0926
Cucsa.338500	Cucurbita moschata (Rifu)	cgycmoB0932
Cucsa.338500	Cucurbita moschata (Rifu)	cgycmoB0939
Cucsa.338500	Wild cucumber (PI 183967)	cgycpiB507
Cucsa.338500	Cucumber (Chinese Long) v2	cgycuB481
Cucsa.338500	Melon (DHL92) v3.5.1	cgymeB550
Cucsa.338500	Watermelon (Charleston Gray)	cgywcgB579
Cucsa.338500	Watermelon (97103) v1	cgywmB609
Cucsa.338500	Cucurbita pepo (Zucchini)	cgycpeB0879
Cucsa.338500	Cucurbita pepo (Zucchini)	cgycpeB0888
Cucsa.338500	Melon (DHL92) v3.6.1	cgymedB551
Cucsa.338500	Silver-seed gourd	carcgyB0321
Cucsa.338500	Silver-seed gourd	carcgyB0506