CmaCh11G011530 (gene) Cucurbita maxima (Rimu)

NameCmaCh11G011530
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptiontRNA (Guanosine(18)-2'-O)-methyltransferase
LocationCma_Chr11 : 6658431 .. 6661359 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCTAGGTGATTAAATTGAATTTAGATCTATAGTCCCAAATGTGCTCATCAATTGGAGCTCATTTGATTTTGACCTATAATTCATATTTCGAAAGGACGCCGGCACTGCATTTGTGGATGAGGAGGGTGTCTATCTCTGCTTCTACAGCTGTCTGAACCATCGAAGCTCTTCAACAAGCAGCCATGGCGTCCTGCAGAATCGCAGTCCGACCTTCACTCTTTCTACTTGGAAGCTGCAGTTCCCGAACAACTCAACTAATTCTCCCTTCTGTTTCCTTCCCCTCACCACCGCTTTCTTCCTACTCTCCATTCTTCACCGCCGTCTCAAACCATTGTACTCTTCTCTCACTTCCTCTCTTTCTCGCTCTCGGAGGCCATACAATTTCTTTTGCATTTCTTTCTTAAATCTGTTCTTGACTTTCCTTCTGTTTTGATTGCTTTATAGCTTCAAGAAACAACCCTAAGGCAGTTTTTACAAGATGTTTTTCCTCGACTTCGGCCGCCGCATCTTCAGTAGAAGTAACAGACGCTGTAGGAAGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACACACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAGGCCGATTAATTTAGATGGAGCTGAATATCCGCACCAAGGGCGATGGTTTCCGTACCTGGATAAGTTTAGCTGTGAAAATTCTTGGTTGAATAGTGGTGAGGTTTTGGACGCCTTGGATCCTCTCATATTGGACTCGAGGAAAGAGAGGTTCAAGAACGTCGTGCGACACAGGAGCTATTCAGTTTGTTTGGTGGTAGAGGGTTTGAGTGATTTCGGCAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAAGAATGATTGAGGTGAAATTGTTTTCGAATACATTTGCCTTTTCCCCCTTGATTTTCCCAATGGAAAATTGTTTGGTTCAGGAATTCGACAGCTGCTTCTAGTCCATTGCGCCGGCGGAATTTCATTCCTCTACATAGTGTGGTTTTACTTCGTTGTTCAATGGAGCACTTGAATTCAATCAATTAGTCAATTTGCATATCTGGTTTTATATGATTAGGAGGTTAAATTGATGAAAATGCATTTGATTTCCATGGTAATTGTTAGATATGGGTGACAATGTGACACATTCTACTTGCTAATAATGACAGAAATTTCCATTGAATACATTTCATTCATGAGTCATAATCTTTCATTCCATGTTTCTTCTTCTGAGATTATTGCAATTTCAATGCAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCAACCCCAGAATGTTTTAGAATTCTGAAATCTCGGGGTTATAGAATAGTATCGACGCATCTTGGATCGAATGCGGTATTGATTCATTTTGTAAACTTTCTCCCTTCCACAATGTCCCATGAAGTAGCTTTACATTGAAATCAATTCTGTTCTTATATCGATTGTCTTATGCCAATTTTATTATTTTTCAACCTTTTTGAGCTTATCCTGACAGCTGAGTTGTGATTGTCCTGTTTTAGGTTTCTGTTTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGGAATGAAAGTAGGTAATTATCTTCTTTGCTTCAATAATACCGAGTTTGATCAAAGTTTTAGTACACTTTTTTAAATCTCTGCTAGCTGGGAGATATCTGTTATATTTAGCAAACCATAACTTAGTCCATGTTTAATAAACTGGCCTACATTTTTGTTGTCCAGCAATTCTAATCCGCTCATCCTTTGATTAGTGAGTTTCGAGTTGTGAATATTGTCCGAATCAGGAGAAATTATACTTGTAGCTAGTTATGATATTTACAATTTATGTTAGTATCTGAGGTTTAGTTCTCTTCGGTCTCAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCACTATTCCTATGAATGGGATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGATCGAACATCTCGACTGGTAACTCTAAAGTCATGTTTGATGTTTTGAATTCGTTTACGCCTAAGTGGTAGTTCAACTAGTTGGGGCGCTACCAGGGGTGTTGTCTGGAAATATGTCGATTCGAGTCTTCCGGTGGGTTCGACACAAAGGATGTCAAAATGGACATATACTGTTTCTAGTACTGAATTGATGGTATGTTCTTTATCAAACTTTTCAGGGCTGCCATGGAGATCTCACATCAATAGAAAGGCAAATTCTACTGGCGGAGTTCTCACTACGGCATAACAAGAACGCAATCAGCGTTGCTCACGAGTATGCGAAGAGGAAGAGTAGTAGCACGAGCTCGGGTTTGGAGGTGTTGCTGTAGAATGGCAAGAGTGGTCTCTTGATGTGTGTAGCAAATGAGTATGAGAAGCAGAAACACACAGAAGAGCCCCCTTCAAAGTTGATCAAAACAGGTAGGCCTGGGATTAAATGGGGATCAGAAAATTTGGAAGCAGAAGAACAATTTCCCCATCTGAAGCATTTTTATCAGCCTTCTTCTTTCTGTGGTAAGCTTCAGCATTCAATTCACATGGCTCTATAATGTTTTACTGTGATTCTGTAATTGACTTGGAGAAACTTTTTAACTTAGTTGTTCATATCAGTTTTTCTTTTCTAATGCTCAAAGATGTCTGTGAAACGCAGTGTCTGGTAATCGAAAAATATGATGCTGAGTTATCTGAAATGCTAAAAGTGAAAATGTAAAAGAGATGGTAATGTGTGGATGATATTATGGATGTTCATATATGTTTCTACTCCTAAGTATTCACGATCACGAG

mRNA sequence

ACCTAGGTGATTAAATTGAATTTAGATCTATAGTCCCAAATGTGCTCATCAATTGGAGCTCATTTGATTTTGACCTATAATTCATATTTCGAAAGGACGCCGGCACTGCATTTGTGGATGAGGAGGGTGTCTATCTCTGCTTCTACAGCTGTCTGAACCATCGAAGCTCTTCAACAAGCAGCCATGGCGTCCTGCAGAATCGCAGTCCGACCTTCACTCTTTCTACTTGGAAGCTGCAGTTCCCGAACAACTCAACTAATTCTCCCTTCTGTTTCCTTCCCCTCACCACCGCTTTCTTCCTACTCTCCATTCTTCACCGCCGTCTCAAACCATTCTTCAAGAAACAACCCTAAGGCAGTTTTTACAAGATGTTTTTCCTCGACTTCGGCCGCCGCATCTTCAGTAGAAGTAACAGACGCTGTAGGAAGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACACACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAGGCCGATTAATTTAGATGGAGCTGAATATCCGCACCAAGGGCGATGGTTTCCGTACCTGGATAAGTTTAGCTGTGAAAATTCTTGGTTGAATAGTGGTGAGGTTTTGGACGCCTTGGATCCTCTCATATTGGACTCGAGGAAAGAGAGGTTCAAGAACGTCGTGCGACACAGGAGCTATTCAGTTTGTTTGGTGGTAGAGGGTTTGAGTGATTTCGGCAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCAACCCCAGAATGTTTTAGAATTCTGAAATCTCGGGGTTATAGAATAGTATCGACGCATCTTGGATCGAATGCGGTTTCTGTTTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGGAATGAAAGTAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCACTATTCCTATGAATGGGATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGATCGAACATCTCGACTGGGCTGCCATGGAGATCTCACATCAATAGAAAGGCAAATTCTACTGGCGGAGTTCTCACTACGGCATAACAAGAACGCAATCAGCGTTGCTCACGAGTATGCGAAGAGGAAGAGTAGTAGCACGAGCTCGGGTTTGGAGGTGTTGCTGTAGAATGGCAAGAGTGGTCTCTTGATGTGTGTAGCAAATGAGTATGAGAAGCAGAAACACACAGAAGAGCCCCCTTCAAAGTTGATCAAAACAGGTAGGCCTGGGATTAAATGGGGATCAGAAAATTTGGAAGCAGAAGAACAATTTCCCCATCTGAAGCATTTTTATCAGCCTTCTTCTTTCTGTGGTAAGCTTCAGCATTCAATTCACATGGCTCTATAATGTTTTACTGTGATTCTGTAATTGACTTGGAGAAACTTTTTAACTTAGTTGTTCATATCAGTTTTTCTTTTCTAATGCTCAAAGATGTCTGTGAAACGCAGTGTCTGGTAATCGAAAAATATGATGCTGAGTTATCTGAAATGCTAAAAGTGAAAATGTAAAAGAGATGGTAATGTGTGGATGATATTATGGATGTTCATATATGTTTCTACTCCTAAGTATTCACGATCACGAG

Coding sequence (CDS)

ATGGCGTCCTGCAGAATCGCAGTCCGACCTTCACTCTTTCTACTTGGAAGCTGCAGTTCCCGAACAACTCAACTAATTCTCCCTTCTGTTTCCTTCCCCTCACCACCGCTTTCTTCCTACTCTCCATTCTTCACCGCCGTCTCAAACCATTCTTCAAGAAACAACCCTAAGGCAGTTTTTACAAGATGTTTTTCCTCGACTTCGGCCGCCGCATCTTCAGTAGAAGTAACAGACGCTGTAGGAAGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACACACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAGGCCGATTAATTTAGATGGAGCTGAATATCCGCACCAAGGGCGATGGTTTCCGTACCTGGATAAGTTTAGCTGTGAAAATTCTTGGTTGAATAGTGGTGAGGTTTTGGACGCCTTGGATCCTCTCATATTGGACTCGAGGAAAGAGAGGTTCAAGAACGTCGTGCGACACAGGAGCTATTCAGTTTGTTTGGTGGTAGAGGGTTTGAGTGATTTCGGCAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCAACCCCAGAATGTTTTAGAATTCTGAAATCTCGGGGTTATAGAATAGTATCGACGCATCTTGGATCGAATGCGGTTTCTGTTTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGGAATGAAAGTAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCACTATTCCTATGAATGGGATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGATCGAACATCTCGACTGGGCTGCCATGGAGATCTCACATCAATAGAAAGGCAAATTCTACTGGCGGAGTTCTCACTACGGCATAACAAGAACGCAATCAGCGTTGCTCACGAGTATGCGAAGAGGAAGAGTAGTAGCACGAGCTCGGGTTTGGAGGTGTTGCTGTAG

Protein sequence

MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVFTRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDGAEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYAKRKSSSTSSGLEVLL
BLAST of CmaCh11G011530 vs. Swiss-Prot
Match: TRMH_AQUAE (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) GN=trmH PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.0e-23
Identity = 56/174 (32.18%), Postives = 102/174 (58.62%), Query Frame = 1

Query: 152 LDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDAS 211
           ++ L+L+ R +R + V+  R   + +  + + +  N SA  R+ DA+G   ++    +  
Sbjct: 3   MEYLVLEKRLKRLREVLEKRQKDLIVFADNVKNEHNFSAIVRTCDAVGVLYLYYYHAEG- 62

Query: 212 KRYRENRHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPT 271
           K+ + N  ++ G+ KW+ IE  ++  +     K+RG++IV+T L   +V+  ++D++ PT
Sbjct: 63  KKAKINEGITQGSHKWVFIEKVDNPVQKLLEFKNRGFQIVATWLSKESVNFREVDYTKPT 122

Query: 272 AIVVGNESRGISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHAVCDRTSR 326
            +VVGNE +G+S E +E++D    IPM GM  S NVSVA GI+++ A   R  +
Sbjct: 123 VLVVGNELQGVSPEIVEIADKKIVIPMYGMAQSLNVSVATGIILYEAQRQREEK 175

BLAST of CmaCh11G011530 vs. Swiss-Prot
Match: TRMH_SHIFL (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri GN=trmH PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.7e-22
Identity = 64/188 (34.04%), Postives = 100/188 (53.19%), Query Frame = 1

Query: 159 SRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 218
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 219 HVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNE 278
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 279 SRGISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSI 338
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 339 ERQILLAE 344
           E+Q LL E
Sbjct: 184 EQQRLLFE 189

BLAST of CmaCh11G011530 vs. Swiss-Prot
Match: TRMH_ECOLI (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) GN=trmH PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.7e-22
Identity = 64/188 (34.04%), Postives = 100/188 (53.19%), Query Frame = 1

Query: 159 SRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 218
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 219 HVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNE 278
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 279 SRGISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSI 338
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 339 ERQILLAE 344
           E+Q LL E
Sbjct: 184 EQQRLLFE 189

BLAST of CmaCh11G011530 vs. Swiss-Prot
Match: TRMH_ECO57 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 GN=trmH PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.7e-22
Identity = 64/188 (34.04%), Postives = 100/188 (53.19%), Query Frame = 1

Query: 159 SRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 218
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 219 HVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNE 278
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 279 SRGISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSI 338
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 339 ERQILLAE 344
           E+Q LL E
Sbjct: 184 EQQRLLFE 189

BLAST of CmaCh11G011530 vs. Swiss-Prot
Match: TRMH_THET2 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=trmH PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.8e-21
Identity = 57/162 (35.19%), Postives = 92/162 (56.79%), Query Frame = 1

Query: 158 DSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDAS-KRYRE 217
           ++R+ R + V+R R   + +++E +    N+SA  R+ DA+G    H V+       + E
Sbjct: 6   EARRRRIEEVLRRRQPDLTVLLENVHKPHNLSAILRTCDAVGVLEAHAVNPTGGVPTFNE 65

Query: 218 NRHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVG 277
               S G+ KW+ + +     E FR LK RG+ + +T L  +A    ++D++ PTA++ G
Sbjct: 66  T---SGGSHKWVYLRVHPDLHEAFRFLKERGFTVYATALREDARDFREVDYTKPTAVLFG 125

Query: 278 NESRGISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHA 319
            E  G+S+EAL L+D    IPM GMV S NVSVAA +++  A
Sbjct: 126 AEKWGVSEEALALADGAIKIPMLGMVQSLNVSVAAAVILFEA 164

BLAST of CmaCh11G011530 vs. TrEMBL
Match: A0A0A0LC15_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G824750 PE=4 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 1.9e-170
Identity = 310/375 (82.67%), Postives = 336/375 (89.60%), Query Frame = 1

Query: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60
           MASCRI  R SLFLL SCS R TQ I  SV  PS  L S+S FFT   N  SR+NP AV 
Sbjct: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT---NRFSRSNPNAVS 60

Query: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           TRCF+S SAA S +EV D V +D+EPPE+ TQDTVEHLLTHS+DVARLMKMERR + LDG
Sbjct: 61  TRCFASISAATSPLEVEDIV-ADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDG 120

Query: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           AEYPH+GRWFPYLDK+SC +SWLNSGE+L+ALD LILDSRKERFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GL+DFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHC+IPMNG
Sbjct: 241 KILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVC+RTSRLGCHGDLTS ERQILLAEFSLRH+K+AIS+A+E A
Sbjct: 301 MVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELA 360

Query: 361 KRKSSSTSSGLEVLL 376
           KRK S+ SS  EVLL
Sbjct: 361 KRKGSAISS-TEVLL 370

BLAST of CmaCh11G011530 vs. TrEMBL
Match: M5WSN7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008118mg PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 1.3e-129
Identity = 246/368 (66.85%), Postives = 281/368 (76.36%), Query Frame = 1

Query: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60
           M++C+  VR SL  + S           S+    PP+                ++P   F
Sbjct: 1   MSACKTLVRTSLSSISS-----------SLRASGPPIPLL-------------HSPSLRF 60

Query: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           +   S    AA   +      S LEPPEI TQDTVE LL   +DV RLMKMER P     
Sbjct: 61  SPLLSPNQEAADFTDEAQFTDSTLEPPEIPTQDTVEQLLKQKNDVGRLMKMERSP----- 120

Query: 121 AEYPHQ--GRWFPYLDKFSC-ENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCL 180
            E  HQ  GRWFPYLDKFSC E+++LNS EVLDALDP ILD RKERF+ VV++RSYSVCL
Sbjct: 121 -ESAHQRGGRWFPYLDKFSCGEDAYLNSAEVLDALDPYILDVRKERFRRVVKNRSYSVCL 180

Query: 181 VVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTP 240
           VVEGL DFGNVSAAFRSADALGFQSVHV+SCD+SKRYR+NRHVSMGAEKWLDIELW+STP
Sbjct: 181 VVEGLGDFGNVSAAFRSADALGFQSVHVLSCDSSKRYRDNRHVSMGAEKWLDIELWDSTP 240

Query: 241 ECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIP 300
           ECF +LKSRGYRI +TH+G +AVSVYDMDWSCPTAIVVGNE+RGISDEAL+LSDLHC+IP
Sbjct: 241 ECFEVLKSRGYRIATTHVGMDAVSVYDMDWSCPTAIVVGNENRGISDEALDLSDLHCSIP 300

Query: 301 MNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAH 360
           M GMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLT  E QILLAEFSLRH+K+++S+AH
Sbjct: 301 MKGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTFEESQILLAEFSLRHSKSSMSIAH 338

Query: 361 EYAKRKSS 366
           EYAKRK++
Sbjct: 361 EYAKRKAA 338

BLAST of CmaCh11G011530 vs. TrEMBL
Match: W9QIA5_9ROSA (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Morus notabilis GN=L484_020971 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 3.8e-126
Identity = 237/365 (64.93%), Postives = 288/365 (78.90%), Query Frame = 1

Query: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60
           MA+C+  V  SL  + S + R+++L L S    + P   +SP  ++     +  N   V 
Sbjct: 1   MAACKTLVSISLSSVAS-TLRSSRLTLSSPH--NSPSLCFSPSISSPQGLRALQN--RVL 60

Query: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           +R ++S   A+ S+E  D+   ++EPPE  TQD +EHLLTH DDV RLMKMERR I+ D 
Sbjct: 61  SRSYASIEGASLSIESIDSP-EEIEPPESPTQDNLEHLLTHKDDVVRLMKMERRRIDTD- 120

Query: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
                 GRWFPYLD+F C +++L+SGEV++AL+P I++SR ERF+NV  +RSYSVCLVVE
Sbjct: 121 ---QRTGRWFPYLDRFKCGDAYLSSGEVVEALEPHIMESRMERFRNVATNRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCD SKRYRENRHVS GAEKWLDIE+W+S  +CF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDGSKRYRENRHVSKGAEKWLDIEVWDSIEDCF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNG 300
           + LKSRGYRI +TH+G + VS+Y+MDWSCPTAIVVGNE+RGISDEAL LSDLHC+IPM G
Sbjct: 241 KALKSRGYRIATTHVGMDVVSIYEMDWSCPTAIVVGNENRGISDEALGLSDLHCSIPMKG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVCDR SRLG HGDLT  ERQILLAEFSLRH K++IS+AHE+A
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRISRLGSHGDLTVEERQILLAEFSLRHGKSSISIAHEFA 355

Query: 361 KRKSS 366
           KRK++
Sbjct: 361 KRKAA 355

BLAST of CmaCh11G011530 vs. TrEMBL
Match: A0A059AR42_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I01991 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 9.4e-125
Identity = 247/375 (65.87%), Postives = 280/375 (74.67%), Query Frame = 1

Query: 1   MASCRIAVRPSLFLL--GSCSSRTTQLILP--SVSFPSPPLSSYSPFFTAVSNHSSRNNP 60
           M+SCR  VR SLF L  G  SS      LP  S+ F SPP                   P
Sbjct: 1   MSSCRTLVRASLFSLDSGRRSSHRASFSLPLRSLHFLSPPSRGLGLL----------RAP 60

Query: 61  KAVFTRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPI 120
                R   S   A + +E T+A   D  P E   +D VE LL   +DV +LMKMERRP 
Sbjct: 61  GGATARRLGSIPGAVA-LEPTEASDED-SPAEGPAKDAVERLLIDREDVTQLMKMERRPA 120

Query: 121 NLDGAEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVC 180
             D       GRWFPYLD+F C  S L+SGEVL+ALDPLI+D RKERF++ VR+RSYSVC
Sbjct: 121 VGDPG-----GRWFPYLDRFRCGASHLSSGEVLEALDPLIMDVRKERFRSAVRNRSYSVC 180

Query: 181 LVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNST 240
           LVVEGL DFGNVSAAFRSADALGFQSVHVVSCD+SKRYRENRHVSMGAEKWLDIELW+ST
Sbjct: 181 LVVEGLCDFGNVSAAFRSADALGFQSVHVVSCDSSKRYRENRHVSMGAEKWLDIELWDST 240

Query: 241 PECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTI 300
            ECFR+L+SRGYRI +THL ++AVS+YDMDWS PTAIVVGNE+RGISDEAL LSDLHC+I
Sbjct: 241 QECFRVLRSRGYRIATTHLATDAVSIYDMDWSYPTAIVVGNENRGISDEALRLSDLHCSI 300

Query: 301 PMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVA 360
           PM GMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLT+ E QILLAEFSLRH+K+AIS+A
Sbjct: 301 PMKGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTTEESQILLAEFSLRHSKSAISIA 358

Query: 361 HEYAKRKSSSTSSGL 372
           HEYA+R+ ++  S L
Sbjct: 361 HEYARRRDATPVSKL 358

BLAST of CmaCh11G011530 vs. TrEMBL
Match: D7SXH6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0108g01380 PE=4 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 4.0e-123
Identity = 236/377 (62.60%), Postives = 285/377 (75.60%), Query Frame = 1

Query: 1   MASCRIAVRPSLFLLGS---CSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPK 60
           M++C+  VR S+  L     C+ R    I      P P   S+SP  +++   S  +   
Sbjct: 1   MSACKTLVRISISSLEFSLVCNQRNFSFI------PLPNHPSFSPISSSLRFASLLSPAS 60

Query: 61  AVFTRCFSSTSAAASSVEVTDAVG---SDLEPPEIHTQDTVEHLLTHSDDVARLMKMERR 120
           ++          +   V V ++VG   +DLE      +D VEHLLT++DDV RLMKMERR
Sbjct: 61  SISLGLTRHKLHSRPYVHVAESVGVETTDLEDDS--NKDIVEHLLTNTDDVGRLMKMERR 120

Query: 121 PINLDGAEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYS 180
            +  DG E     RWFPYLDKF    ++L+S E+L+A+DP I++ RKERF+NVV+ RSYS
Sbjct: 121 SMTGDGGELIQSKRWFPYLDKFKTGTTFLSSSEILEAMDPYIMEVRKERFRNVVKSRSYS 180

Query: 181 VCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWN 240
           VCLVVEGL DFGNVSAAFRSADALGFQSVHVVSCD+SKRY++NRHVSMGAEKWLDIELWN
Sbjct: 181 VCLVVEGLCDFGNVSAAFRSADALGFQSVHVVSCDSSKRYKDNRHVSMGAEKWLDIELWN 240

Query: 241 STPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHC 300
           ST ECF +LKSRGYRI +TH+G +AVS+YDMDW+CPTAIVVGNE+RGISDEALELSDLHC
Sbjct: 241 STQECFEVLKSRGYRIATTHVGMDAVSIYDMDWTCPTAIVVGNENRGISDEALELSDLHC 300

Query: 301 TIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAIS 360
           +IPM GMVDSFNVSVAAGILMHHAVCDRTSRLG HGDL+  E +ILLAEFSLRH+K+A S
Sbjct: 301 SIPMKGMVDSFNVSVAAGILMHHAVCDRTSRLGSHGDLSLEESRILLAEFSLRHSKSANS 360

Query: 361 VAHEYAKRKSSSTSSGL 372
           +AHE+AKRK++S  S L
Sbjct: 361 IAHEFAKRKAASPVSKL 369

BLAST of CmaCh11G011530 vs. TAIR10
Match: AT5G15390.1 (AT5G15390.1 tRNA/rRNA methyltransferase (SpoU) family protein)

HSP 1 Score: 390.2 bits (1001), Expect = 1.4e-108
Identity = 210/365 (57.53%), Postives = 260/365 (71.23%), Query Frame = 1

Query: 15  LGSCSS--RTTQLILPSVSFPSPPLS-------SYSPFFTAVSNHSSRNNPKAVFTRCFS 74
           + SC S  R +    P V  P+P  S       ++SP      N S     +  F     
Sbjct: 1   MNSCKSLIRASISSFPFVPLPNPNFSITFISVRAFSPLSVLHPNSSCIVTARRTF----- 60

Query: 75  STSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLT-HSDDVARLMKMERRPINLDGAEYP 134
             + A S   +T+      E P+    DTV+ LLT +  + + LMKMERR  +L   E  
Sbjct: 61  HGAVALSPESLTE------ESPK----DTVKGLLTTNRGEASSLMKMERR-CSLSNGEGD 120

Query: 135 HQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEGLSD 194
            +G WFPY D+F C    L+S EVL+A+ P +++ R +RF+ VV +RSYSVCLVVEGLSD
Sbjct: 121 CRGSWFPYEDRFRCGEVHLSSREVLEAVSPHMMEERTDRFRRVVENRSYSVCLVVEGLSD 180

Query: 195 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFRILK 254
           FGN+SAAFRSADALG QSVHVVSCD+SKRY  NRHVSMGAEKWLDIE W++  ECF++LK
Sbjct: 181 FGNISAAFRSADALGIQSVHVVSCDSSKRYNGNRHVSMGAEKWLDIEFWDTPKECFKVLK 240

Query: 255 SRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNGMVDS 314
           SRGYRI +THLG + VS+YDMDWSCPTAIVVGNE RGISDEALELSDL C+IPMNGMVDS
Sbjct: 241 SRGYRIATTHLGMDTVSIYDMDWSCPTAIVVGNEGRGISDEALELSDLRCSIPMNGMVDS 300

Query: 315 FNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYAKRKS 370
           FNVSVAAGILMHHAV DRT+RLG HGDL+  E++IL+AEFSLRH++++I +A+E+AKRK 
Sbjct: 301 FNVSVAAGILMHHAVSDRTTRLGSHGDLSEAEKEILMAEFSLRHSRSSICIAYEFAKRKQ 349

BLAST of CmaCh11G011530 vs. TAIR10
Match: AT2G19870.1 (AT2G19870.1 tRNA/rRNA methyltransferase (SpoU) family protein)

HSP 1 Score: 48.9 bits (115), Expect = 7.9e-06
Identity = 47/202 (23.27%), Postives = 86/202 (42.57%), Query Frame = 1

Query: 144 NSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSV 203
           + G VLDA  PL L   KE          YS+ + ++ ++D  N+ A  RSA   G   V
Sbjct: 388 HQGLVLDA-SPLELVKVKELDPISSEEEKYSLWVALDEVTDPQNLGAIIRSAYFFGATGV 447

Query: 204 HVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVY 263
            V + +++         S G+ + +++    +  +        G+R+V   +   AV++ 
Sbjct: 448 VVCAKNSAPLSAVVSKASAGSLEVMELRYCKNMMQFLEASAENGWRVVGGSVSPKAVALN 507

Query: 264 DMDWSCPTAIV---VGNESRGISDEAL-ELSDLHCTIPMNGM-----------------V 323
           ++    PT +V    G   R + + +  +L  +   +P                     V
Sbjct: 508 EVLPGSPTILVLGNEGTGLRPLVERSCTDLVRISGNMPNEVAVTESDDAEGEGFRSFLAV 567

Query: 324 DSFNVSVAAGILMHHAVCDRTS 325
           +S NVSVAAG+ +HH + ++ S
Sbjct: 568 ESLNVSVAAGLFLHHLIGNKAS 588

BLAST of CmaCh11G011530 vs. NCBI nr
Match: gi|700204397|gb|KGN59530.1| (hypothetical protein Csa_3G824750 [Cucumis sativus])

HSP 1 Score: 606.7 bits (1563), Expect = 2.8e-170
Identity = 310/375 (82.67%), Postives = 336/375 (89.60%), Query Frame = 1

Query: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60
           MASCRI  R SLFLL SCS R TQ I  SV  PS  L S+S FFT   N  SR+NP AV 
Sbjct: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT---NRFSRSNPNAVS 60

Query: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           TRCF+S SAA S +EV D V +D+EPPE+ TQDTVEHLLTHS+DVARLMKMERR + LDG
Sbjct: 61  TRCFASISAATSPLEVEDIV-ADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDG 120

Query: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           AEYPH+GRWFPYLDK+SC +SWLNSGE+L+ALD LILDSRKERFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GL+DFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHC+IPMNG
Sbjct: 241 KILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVC+RTSRLGCHGDLTS ERQILLAEFSLRH+K+AIS+A+E A
Sbjct: 301 MVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELA 360

Query: 361 KRKSSSTSSGLEVLL 376
           KRK S+ SS  EVLL
Sbjct: 361 KRKGSAISS-TEVLL 370

BLAST of CmaCh11G011530 vs. NCBI nr
Match: gi|778685436|ref|XP_004136796.2| (PREDICTED: uncharacterized protein LOC101218911 isoform X1 [Cucumis sativus])

HSP 1 Score: 606.7 bits (1563), Expect = 2.8e-170
Identity = 310/375 (82.67%), Postives = 336/375 (89.60%), Query Frame = 1

Query: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60
           MASCRI  R SLFLL SCS R TQ I  SV  PS  L S+S FFT   N  SR+NP AV 
Sbjct: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT---NRFSRSNPNAVS 103

Query: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           TRCF+S SAA S +EV D V +D+EPPE+ TQDTVEHLLTHS+DVARLMKMERR + LDG
Sbjct: 104 TRCFASISAATSPLEVEDIV-ADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDG 163

Query: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           AEYPH+GRWFPYLDK+SC +SWLNSGE+L+ALD LILDSRKERFKNVVR+RSYSVCLVVE
Sbjct: 164 AEYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVE 223

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GL+DFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 224 GLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 283

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHC+IPMNG
Sbjct: 284 KILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNG 343

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVC+RTSRLGCHGDLTS ERQILLAEFSLRH+K+AIS+A+E A
Sbjct: 344 MVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELA 403

Query: 361 KRKSSSTSSGLEVLL 376
           KRK S+ SS  EVLL
Sbjct: 404 KRKGSAISS-TEVLL 413

BLAST of CmaCh11G011530 vs. NCBI nr
Match: gi|659085333|ref|XP_008443364.1| (PREDICTED: uncharacterized protein LOC103486969 [Cucumis melo])

HSP 1 Score: 593.2 bits (1528), Expect = 3.2e-166
Identity = 303/369 (82.11%), Postives = 330/369 (89.43%), Query Frame = 1

Query: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60
           MASCRI  R SL LL S S R TQ + PSV  PS  L S SPFFT   N  SR+NP AV 
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFT---NTLSRSNPNAVS 60

Query: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           +RCF+S SAA S +E+ D V +D+EPPE+ TQDTVEHLLTHSDDV+RLMKMERRP+ LDG
Sbjct: 61  SRCFASISAATSPLELADIV-ADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDG 120

Query: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           AEYPH+GRWFPYLDK+S  +SWLNSGE+L+ALD LILD+RKERFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNST ECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTSECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHC+IPMNG
Sbjct: 241 KILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTS ERQILLAEFSLRH+K+AIS+A+E A
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELA 360

Query: 361 KRKSSSTSS 370
           KRK S+ SS
Sbjct: 361 KRKGSAISS 364

BLAST of CmaCh11G011530 vs. NCBI nr
Match: gi|778685447|ref|XP_011652219.1| (PREDICTED: uncharacterized protein LOC101218911 isoform X2 [Cucumis sativus])

HSP 1 Score: 590.1 bits (1520), Expect = 2.7e-165
Identity = 305/375 (81.33%), Postives = 330/375 (88.00%), Query Frame = 1

Query: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60
           MASCRI  R SLFLL SCS R TQ I  SV  PS  L S+S FFT   N  S        
Sbjct: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT---NRFS-------- 103

Query: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           TRCF+S SAA S +EV D V +D+EPPE+ TQDTVEHLLTHS+DVARLMKMERR + LDG
Sbjct: 104 TRCFASISAATSPLEVEDIV-ADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDG 163

Query: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           AEYPH+GRWFPYLDK+SC +SWLNSGE+L+ALD LILDSRKERFKNVVR+RSYSVCLVVE
Sbjct: 164 AEYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVE 223

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GL+DFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 224 GLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 283

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHC+IPMNG
Sbjct: 284 KILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNG 343

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVC+RTSRLGCHGDLTS ERQILLAEFSLRH+K+AIS+A+E A
Sbjct: 344 MVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELA 403

Query: 361 KRKSSSTSSGLEVLL 376
           KRK S+ SS  EVLL
Sbjct: 404 KRKGSAISS-TEVLL 405

BLAST of CmaCh11G011530 vs. NCBI nr
Match: gi|595938113|ref|XP_007215633.1| (hypothetical protein PRUPE_ppa008118mg [Prunus persica])

HSP 1 Score: 471.1 bits (1211), Expect = 1.8e-129
Identity = 246/368 (66.85%), Postives = 281/368 (76.36%), Query Frame = 1

Query: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60
           M++C+  VR SL  + S           S+    PP+                ++P   F
Sbjct: 1   MSACKTLVRTSLSSISS-----------SLRASGPPIPLL-------------HSPSLRF 60

Query: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           +   S    AA   +      S LEPPEI TQDTVE LL   +DV RLMKMER P     
Sbjct: 61  SPLLSPNQEAADFTDEAQFTDSTLEPPEIPTQDTVEQLLKQKNDVGRLMKMERSP----- 120

Query: 121 AEYPHQ--GRWFPYLDKFSC-ENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCL 180
            E  HQ  GRWFPYLDKFSC E+++LNS EVLDALDP ILD RKERF+ VV++RSYSVCL
Sbjct: 121 -ESAHQRGGRWFPYLDKFSCGEDAYLNSAEVLDALDPYILDVRKERFRRVVKNRSYSVCL 180

Query: 181 VVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTP 240
           VVEGL DFGNVSAAFRSADALGFQSVHV+SCD+SKRYR+NRHVSMGAEKWLDIELW+STP
Sbjct: 181 VVEGLGDFGNVSAAFRSADALGFQSVHVLSCDSSKRYRDNRHVSMGAEKWLDIELWDSTP 240

Query: 241 ECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIP 300
           ECF +LKSRGYRI +TH+G +AVSVYDMDWSCPTAIVVGNE+RGISDEAL+LSDLHC+IP
Sbjct: 241 ECFEVLKSRGYRIATTHVGMDAVSVYDMDWSCPTAIVVGNENRGISDEALDLSDLHCSIP 300

Query: 301 MNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAH 360
           M GMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLT  E QILLAEFSLRH+K+++S+AH
Sbjct: 301 MKGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTFEESQILLAEFSLRHSKSSMSIAH 338

Query: 361 EYAKRKSS 366
           EYAKRK++
Sbjct: 361 EYAKRKAA 338

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TRMH_AQUAE1.0e-2332.18tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) GN=... [more]
TRMH_SHIFL5.7e-2234.04tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri GN=trmH PE=3 SV... [more]
TRMH_ECOLI5.7e-2234.04tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) GN=... [more]
TRMH_ECO575.7e-2234.04tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 GN=trmH ... [more]
TRMH_THET22.8e-2135.19tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain HB27... [more]
Match NameE-valueIdentityDescription
A0A0A0LC15_CUCSA1.9e-17082.67Uncharacterized protein OS=Cucumis sativus GN=Csa_3G824750 PE=4 SV=1[more]
M5WSN7_PRUPE1.3e-12966.85Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008118mg PE=4 SV=1[more]
W9QIA5_9ROSA3.8e-12664.93tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Morus notabilis GN=L484_020971 PE... [more]
A0A059AR42_EUCGR9.4e-12565.87Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I01991 PE=4 SV=1[more]
D7SXH6_VITVI4.0e-12362.60Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0108g01380 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT5G15390.11.4e-10857.53 tRNA/rRNA methyltransferase (SpoU) family protein[more]
AT2G19870.17.9e-0623.27 tRNA/rRNA methyltransferase (SpoU) family protein[more]
Match NameE-valueIdentityDescription
gi|700204397|gb|KGN59530.1|2.8e-17082.67hypothetical protein Csa_3G824750 [Cucumis sativus][more]
gi|778685436|ref|XP_004136796.2|2.8e-17082.67PREDICTED: uncharacterized protein LOC101218911 isoform X1 [Cucumis sativus][more]
gi|659085333|ref|XP_008443364.1|3.2e-16682.11PREDICTED: uncharacterized protein LOC103486969 [Cucumis melo][more]
gi|778685447|ref|XP_011652219.1|2.7e-16581.33PREDICTED: uncharacterized protein LOC101218911 isoform X2 [Cucumis sativus][more]
gi|595938113|ref|XP_007215633.1|1.8e-12966.85hypothetical protein PRUPE_ppa008118mg [Prunus persica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001537SpoU_MeTrfase
Vocabulary: Molecular Function
TermDefinition
GO:0008168methyltransferase activity
GO:0003723RNA binding
GO:0008173RNA methyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0030488tRNA methylation
GO:0006396RNA processing
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
biological_process GO:0001510 RNA methylation
biological_process GO:0006396 RNA processing
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003723 RNA binding
molecular_function GO:0009020 tRNA (guanosine-2'-O-)-methyltransferase activity
molecular_function GO:0008173 RNA methyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G011530.1CmaCh11G011530.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001537tRNA/rRNA methyltransferase, SpoU typePFAMPF00588SpoU_methylasecoord: 175..316
score: 3.5
NoneNo IPR availablePANTHERPTHR12029RNA METHYLTRANSFERASEcoord: 88..343
score: 1.4E
NoneNo IPR availablePANTHERPTHR12029:SF45RRNA METHYLASE-LIKE PROTEINcoord: 88..343
score: 1.4E