Lsi03G007850 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G007850
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Heavy metal transport/detoxification superfamily protein
Location	chr03 : 11373500 .. 11375451 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTATGTGTGTGGTCCAACCAATCACCATCCAAACCCAGGAAGAAAAAAAAAATTGATCAGAGAGCATTGTGCCATTTCTATCACACCCTCTGATCCCCATTAACCTCTGGAAAAGACAACCTCAACAATTTCATCATATAAATTCTGCCTCTAACTCTGTTTCTTCTACTACTCCACCATATAAACCATGAAGAAGCTTGGTGGATTCATGTGCCACTCTCCGGCTGCCACCGCAGTCTGCATCCCAGCAAATCCCCGCTCCGCAGTTGTGTCGAGAAGGCTGGCTAGAGCCGACGATGTTAATAACGCCAATGGCAACCGTGCGAGGTTAGTGAGCAATGCTGTGAAGTACTCGAAGCTTGTCAAGTGTTCGAGCTTGAGTGTTCGGAGCGAGGGAACGGGGACCGTCATGAAGAAACCGGTGCCCATTATGCTTTCTTCGCGGCCAGAGAATGTCTTCCATGTGGTGGTTATGAGGGTGGCGCTTCACTGCCAAGGCTGTGCTAGTAAAGTTAAGAGACATCTCACCAAAATGGAAGGTACTATTTCATATTCATGATATTATTTCTATCTTGTAGTCGTTATTTCAATTTTAATGATTATATCTATATGGATATATATTATTGGTAAACTTGAAATTTCAAATATATATATTGATAAAATATTGACCTCTGCAAACATCCTATTGATTAAAAAATATATAAAAATTATTAATAATTAATAATTATTTTTTTTCTCTAAATGAGTTACAAACCTTAATTTAAAATATTTTATGTGGTTGGCGTATAAGATTCTGTCAATTTTTTTTTTTTAATATTTGCTGACCATCACAAAATATAAAAAAAATAGTGATTATTTATAATATTGATGTTAAACTCTTCGATTTATGATATATCGAAAAATACATTGAGATATAACCAACTATTTTAATTCTTAGTTGAGAGTATAAATAAAAAATAAATAAAGTGGTAATACTATTATTTCTTTTTTAATAATTTATGTATTATAAAACAAAATATGGCGAACAAGTATTAATTATACTTCCTTCTTAATTTTTTATCATTGTAAAAAACATTTCATAATTATATTAATAAGAGCCTATTGTTGTTCTTTAAGCATTATCTCAAAGTAAATATAACTCAATTGATAAATAAATGTGTTAGGGACCAAGAGATCTGCAATAATTCGAATTTTTCTACTTCTATTGTACTTAAAAAAATTACTATTATTTTACATAAATATAAAACTTTTTTAAAAAAAAAATGTAATTTCTTTAATTATTATTATTTTTTGATAGACTTAATTATTATTATTTAATGACATGCAAAAGTTTCTCCTTTTGTAATATATACATATATAATGTTACATTTTTCATTGGTTTCTAACCGAGATTTTGACCTTAAATTTCGAGTTTAATAATTGCTAAAACTCAAAAGAAAAATACAAAAATAGAACTTTTTAAATTTTAGTGAGAAAAATGTAAAAAAAGAAAAATTAAGGATGAAAGTGAAATTTATATTTTTGTTTTACTAAAATTCTATTGTTGTACAGTGTAATGAAATGACATAACATCTGGGTATGAATGTAATTGGCAAATGACAGGAGTGACATCATTCAGCATAGATCTGGAGGCCAAGAGAGTGACTGTGATGGGACATATTTCACCGCTGGGTGTTTTAGAGAGTATTTCTAAGGTGAAAAGAGCTGAGTTATGGTCAATGTTAAACTAATTCATTTTATAAATAAATGGCTTCTGGCTGTGACAGAACAGTGTGACTGTGAGGGAAAAGATTAGTGAAATGACCAAAGTGGGACAGCAAAAGGAGGAAAGTGTGTGTAATTTGTAAGGCACCCAGGAGGAGTTATGTGTAATTTCAATCTTTTTGTTGGACAAATTATCCATTTTATTAGTGGTGGTCCTAAATTCCAAGGGCACCAACTTTTTTTAAA

mRNA sequence

ATTTATGTGTGTGGTCCAACCAATCACCATCCAAACCCAGGAAGAAAAAAAAAATTGATCAGAGAGCATTGTGCCATTTCTATCACACCCTCTGATCCCCATTAACCTCTGGAAAAGACAACCTCAACAATTTCATCATATAAATTCTGCCTCTAACTCTGTTTCTTCTACTACTCCACCATATAAACCATGAAGAAGCTTGGTGGATTCATGTGCCACTCTCCGGCTGCCACCGCAGTCTGCATCCCAGCAAATCCCCGCTCCGCAGTTGTGTCGAGAAGGCTGGCTAGAGCCGACGATGTTAATAACGCCAATGGCAACCGTGCGAGGTTAGTGAGCAATGCTGTGAAGTACTCGAAGCTTGTCAAGTGTTCGAGCTTGAGTGTTCGGAGCGAGGGAACGGGGACCGTCATGAAGAAACCGGTGCCCATTATGCTTTCTTCGCGGCCAGAGAATGTCTTCCATGTGGTGGTTATGAGGGTGGCGCTTCACTGCCAAGGCTGTGCTAGTAAAGTTAAGAGACATCTCACCAAAATGGAAGGAGTGACATCATTCAGCATAGATCTGGAGGCCAAGAGAGTGACTGTGATGGGACATATTTCACCGCTGGGTGTTTTAGAGAGTATTTCTAAGGTGAAAAGAGCTGAGTTATGGTCAATGTTAAACTAATTCATTTTATAAATAAATGGCTTCTGGCTGTGACAGAACAGTGTGACTGTGAGGGAAAAGATTAGTGAAATGACCAAAGTGGGACAGCAAAAGGAGGAAAGTGTGTGTAATTTGTAAGGCACCCAGGAGGAGTTATGTGTAATTTCAATCTTTTTGTTGGACAAATTATCCATTTTATTAGTGGTGGTCCTAAATTCCAAGGGCACCAACTTTTTTTAAA

Coding sequence (CDS)

ATGAAGAAGCTTGGTGGATTCATGTGCCACTCTCCGGCTGCCACCGCAGTCTGCATCCCAGCAAATCCCCGCTCCGCAGTTGTGTCGAGAAGGCTGGCTAGAGCCGACGATGTTAATAACGCCAATGGCAACCGTGCGAGGTTAGTGAGCAATGCTGTGAAGTACTCGAAGCTTGTCAAGTGTTCGAGCTTGAGTGTTCGGAGCGAGGGAACGGGGACCGTCATGAAGAAACCGGTGCCCATTATGCTTTCTTCGCGGCCAGAGAATGTCTTCCATGTGGTGGTTATGAGGGTGGCGCTTCACTGCCAAGGCTGTGCTAGTAAAGTTAAGAGACATCTCACCAAAATGGAAGGAGTGACATCATTCAGCATAGATCTGGAGGCCAAGAGAGTGACTGTGATGGGACATATTTCACCGCTGGGTGTTTTAGAGAGTATTTCTAAGGTGAAAAGAGCTGAGTTATGGTCAATGTTAAACTAA

Protein sequence

MKKLGGFMCHSPAATAVCIPANPRSAVVSRRLARADDVNNANGNRARLVSNAVKYSKLVKCSSLSVRSEGTGTVMKKPVPIMLSSRPENVFHVVVMRVALHCQGCASKVKRHLTKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWSMLN

Homology

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BLAST of Lsi03G007850 vs. Swiss-Prot
Match: ATOX1_ARATH (Copper transport protein ATX1 OS=Arabidopsis thaliana GN=ATX1 PE=1 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 4.7e-10
Identity = 30/63 (47.62%), Postives = 45/63 (71.43%), Query Frame = 1


Query: 94  VVMRVALHCQGCASKVKRHLTKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKV-KRA 153
           VV+RVA+ C+GC   VKR L KMEGV SF +D++ ++VTV G++ P  VL++++K  K+ 
Sbjct: 5   VVLRVAMTCEGCVGAVKRVLGKMEGVESFDVDIKEQKVTVKGNVQPDAVLQTVTKTGKKT 64

Query: 154 ELW 156
             W
Sbjct: 65  AFW 67

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BLAST of Lsi03G007850 vs. Swiss-Prot
Match: CCH_ARATH (Copper transport protein CCH OS=Arabidopsis thaliana GN=CCH PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.0e-09
Identity = 29/63 (46.03%), Postives = 42/63 (66.67%), Query Frame = 1


Query: 94  VVMRVALHCQGCASKVKRHLTKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKV-KRA 153
           VV++V + CQGC   V R L KMEGV SF ID++ ++VTV G++ P  V +++SK  K+ 
Sbjct: 5   VVLKVGMSCQGCVGAVNRVLGKMEGVESFDIDIKEQKVTVKGNVEPEAVFQTVSKTGKKT 64

Query: 154 ELW 156
             W
Sbjct: 65  SYW 67

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BLAST of Lsi03G007850 vs. Swiss-Prot
Match: HIP20_ARATH (Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana GN=HIPP20 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 2.4e-06
Identity = 25/71 (35.21%), Postives = 42/71 (59.15%), Query Frame = 1


Query: 86  RPENVFHVVVMRVALHCQGCASKVKRHLTKMEGVTSFSIDLEAKRVTVMGHISPLGVLES 145
           R   V   V ++V + C GC  +VK  ++ M+GV S  ++ +  +VTV G++ P  VL+ 
Sbjct: 22  RKRKVMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEVNRKIHKVTVSGYVEPKKVLKR 81

Query: 146 ISKV-KRAELW 156
           I +  K+AE+W
Sbjct: 82  IERTGKKAEIW 92

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BLAST of Lsi03G007850 vs. TrEMBL
Match: A0A0A0K2I2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G060180 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 6.7e-56
Identity = 130/165 (78.79%), Postives = 140/165 (84.85%), Query Frame = 1


Query: 1   MKKLGGFMCHSPAATAVCIPANPRSAVVSRRLARADDVNNA---NGNR-ARLVSN-AVKY 60
           MKKLGGFMCHS A TAVCIP++PRS VVSRRLAR D  NN    NGNR AR VSN  VKY
Sbjct: 1   MKKLGGFMCHSAATTAVCIPSDPRSTVVSRRLARPDYANNNHANNGNRSARFVSNNTVKY 60

Query: 61  SKLVKCS-SLSVRSEGTGTVMKKP-VPIMLSSRPEN--VFHVVVMRVALHCQGCASKVKR 120
           SKLV+ S SLSVRSEG    +KKP +P+MLSS  EN  VFHVVVMRVALHCQGCASKVKR
Sbjct: 61  SKLVESSPSLSVRSEGKHINLKKPAMPVMLSSPAENNNVFHVVVMRVALHCQGCASKVKR 120

Query: 121 HLTKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWS 157
           HL++MEGVTSFSIDLEAKRVTVMGHISPLGVL+SISKVKRAELW+
Sbjct: 121 HLSRMEGVTSFSIDLEAKRVTVMGHISPLGVLQSISKVKRAELWA 165

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BLAST of Lsi03G007850 vs. TrEMBL
Match: V7CNZ1_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G207200g PE=4 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 3.8e-35
Identity = 88/158 (55.70%), Postives = 111/158 (70.25%), Query Frame = 1


Query: 6   GFMCHSPAATAVCIPA-NPRSAVVSRRLARADDVNNANGNRARLVSNAVKYSKLVK---- 65
           GFMCHS A+TAVC+   +PR  VV +RL R+  +++      RL+ N  KYSKLV+    
Sbjct: 14  GFMCHSQASTAVCMSTRDPRPVVVPKRLERSVFLDDT-----RLI-NYAKYSKLVEPPRS 73

Query: 66  --CSSLSVRSEGTGTVMKKPVPIMLSSRPENVFHVVVMRVALHCQGCASKVKRHLTKMEG 125
                + +R +       +P  ++ +    NVF VVVMRVA+HCQGCA KVK+HL+KMEG
Sbjct: 74  NPVPKIKLRGQDQDQAKNEPRELLKTQTDNNVFQVVVMRVAIHCQGCAGKVKKHLSKMEG 133

Query: 126 VTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWS 157
           VTSFSIDLE+KRVTVMGHISP+ VLESISKVKRAELW+
Sbjct: 134 VTSFSIDLESKRVTVMGHISPVAVLESISKVKRAELWT 165

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BLAST of Lsi03G007850 vs. TrEMBL
Match: A0A0L9TMD1_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g087400 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 6.6e-35
Identity = 88/158 (55.70%), Postives = 111/158 (70.25%), Query Frame = 1


Query: 6   GFMCHSPAATAVCIPA-NPRSAVVSRRLARADDVNNANGNRARLVSNAVKYSKLVK---- 65
           GFMCHS A+TAVC+   +PRS V+ +RL R   +++      RL+ N  KYSKLV+    
Sbjct: 13  GFMCHSQASTAVCMSTRDPRSVVLPKRLERTVFLDDT-----RLI-NYAKYSKLVEPPRS 72

Query: 66  --CSSLSVRSEGTGTVMKKPVPIMLSSRPENVFHVVVMRVALHCQGCASKVKRHLTKMEG 125
                + +R +       +P  ++ +    NVF VVVMRVA+HCQGCA KVK+HL+KMEG
Sbjct: 73  NPVPKIKLRVQDQDQAKNQPKDLLKTQTDNNVFQVVVMRVAIHCQGCAVKVKKHLSKMEG 132

Query: 126 VTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWS 157
           VTSFSIDLE+KRVTVMGHISP+ VLESISKVKRAELW+
Sbjct: 133 VTSFSIDLESKRVTVMGHISPVAVLESISKVKRAELWT 164

[Close]

BLAST of Lsi03G007850 vs. TrEMBL
Match: A0A0S3R048_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G156700 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 6.6e-35
Identity = 88/158 (55.70%), Postives = 111/158 (70.25%), Query Frame = 1


Query: 6   GFMCHSPAATAVCIPA-NPRSAVVSRRLARADDVNNANGNRARLVSNAVKYSKLVK---- 65
           GFMCHS A+TAVC+   +PRS V+ +RL R   +++      RL+ N  KYSKLV+    
Sbjct: 13  GFMCHSQASTAVCMSTRDPRSVVLPKRLERTVFLDDT-----RLI-NYAKYSKLVEPPRS 72

Query: 66  --CSSLSVRSEGTGTVMKKPVPIMLSSRPENVFHVVVMRVALHCQGCASKVKRHLTKMEG 125
                + +R +       +P  ++ +    NVF VVVMRVA+HCQGCA KVK+HL+KMEG
Sbjct: 73  NPVPKIKLRVQDQDQAKNQPKDLLKTQTDNNVFQVVVMRVAIHCQGCAVKVKKHLSKMEG 132

Query: 126 VTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWS 157
           VTSFSIDLE+KRVTVMGHISP+ VLESISKVKRAELW+
Sbjct: 133 VTSFSIDLESKRVTVMGHISPVAVLESISKVKRAELWT 164

[Close]

BLAST of Lsi03G007850 vs. TrEMBL
Match: F6H1N1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0055g00930 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.3e-34
Identity = 93/171 (54.39%), Postives = 114/171 (66.67%), Query Frame = 1


Query: 6   GFMCHSPAATAVCIPANPRSAVVSRRLARADDVNNANGNRARLVSNAVKYSKLVKCSSLS 65
           G MCHSPAATAVCIP +PRS +V RR  R         + +RL++NA KYS+LV+    +
Sbjct: 3   GLMCHSPAATAVCIPGDPRSVIVPRRPDRT------LVDHSRLINNA-KYSRLVESHRFT 62

Query: 66  VRSEGTGTV--MKK-------PVPIMLSSRPENVFHV-----------VVMRVALHCQGC 125
             ++ +  V  MK+       P+P    S   ++ HV           VVMRV+LHCQGC
Sbjct: 63  PAAKRSVLVPAMKRGHRPSAEPMPFHPPSSTPSLDHVFQVRFMIICKVVVMRVSLHCQGC 122

Query: 126 ASKVKRHLTKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWS 157
           A KVK+HL+KMEGVTSFSIDLE KRVTVMGH+SP GVLESISKVK+AELWS
Sbjct: 123 AGKVKKHLSKMEGVTSFSIDLETKRVTVMGHVSPSGVLESISKVKKAELWS 166

[Close]

BLAST of Lsi03G007850 vs. TAIR10
Match: AT2G28660.1 (AT2G28660.1 Chloroplast-targeted copper chaperone protein)

HSP 1 Score: 97.1 bits (240), Expect = 1.1e-20
Identity = 47/94 (50.00%), Postives = 67/94 (71.28%), Query Frame = 1


Query: 62  SSLSVRSEGTGTVMKKPVPIMLSSRPENVFHVVVMRVALHCQGCASKVKRHLTKMEGVTS 121
           SS S  S  + +     V  + S  P     VVV+RV++HC+GC  KV++H++KMEGVTS
Sbjct: 153 SSASSSSSSSSSSFSSSVTNVSSPAPSTDDQVVVLRVSIHCKGCEGKVRKHISKMEGVTS 212

Query: 122 FSIDLEAKRVTVMGHISPLGVLESISKVKRAELW 156
           ++IDL  K+VTV+G I+P+G++ESISKVK A+LW
Sbjct: 213 YTIDLATKKVTVVGKITPVGLVESISKVKFAQLW 246

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BLAST of Lsi03G007850 vs. TAIR10
Match: AT5G02600.2 (AT5G02600.2 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 5.9e-19
Identity = 41/63 (65.08%), Postives = 54/63 (85.71%), Query Frame = 1


Query: 93  VVVMRVALHCQGCASKVKRHLTKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRA 152
           VVV+RV+LHC+GCA KVK+HL+K++GVTS++ID  AK+VTV G ++PL VL SISKVK A
Sbjct: 251 VVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLTVLASISKVKNA 310

Query: 153 ELW 156
           + W
Sbjct: 311 QFW 313

[Close]

BLAST of Lsi03G007850 vs. TAIR10
Match: AT2G37390.1 (AT2G37390.1 Chloroplast-targeted copper chaperone protein)

HSP 1 Score: 89.7 bits (221), Expect = 1.7e-18
Identity = 39/64 (60.94%), Postives = 54/64 (84.38%), Query Frame = 1


Query: 93  VVVMRVALHCQGCASKVKRHLTKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRA 152
           VVV++V+LHC+GC  KV++HL +M+GVTSF+ID  AK+VTV G I+PL +L+SISKVK A
Sbjct: 182 VVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLEILDSISKVKNA 241

Query: 153 ELWS 157
           + W+
Sbjct: 242 QFWT 245

[Close]

BLAST of Lsi03G007850 vs. TAIR10
Match: AT3G24450.1 (AT3G24450.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 1.4e-17
Identity = 41/93 (44.09%), Postives = 64/93 (68.82%), Query Frame = 1


Query: 68  SEGTGTVMKKPVPIMLSSRPENVFH----VVVMRVALHCQGCASKVKRHLTKMEGVTSFS 127
           +E +G VM+    +    R    FH    +V ++V++HC GCA KV++H++K++GVT + 
Sbjct: 46  TEKSGKVMRLKDVVAADHRQTLAFHLKPKIVELKVSMHCYGCAKKVEKHISKLDGVTWYK 105

Query: 128 IDLEAKRVTVMGHISPLGVLESISKVKRAELWS 157
           ++LE+K+V V G+I P+ VLESI KVK A+LWS
Sbjct: 106 VELESKKVVVKGNILPVDVLESICKVKNAQLWS 138

[Close]

BLAST of Lsi03G007850 vs. TAIR10
Match: AT3G53530.2 (AT3G53530.2 Chloroplast-targeted copper chaperone protein)

HSP 1 Score: 79.0 bits (193), Expect = 3.0e-15
Identity = 40/67 (59.70%), Postives = 53/67 (79.10%), Query Frame = 1


Query: 93  VVVMRVALHC--QGCASKVKRHLTKME-GVTSFSIDLEAKRVTVMGHISPLGVLESISKV 152
           VVV+RV+LHC  +GC  KVK+HL+KM+ GVTSF+ID  +K+VTV G I+PL VL  +SKV
Sbjct: 169 VVVLRVSLHCHCRGCQGKVKKHLSKMQVGVTSFNIDFASKKVTVTGDITPLEVLGCLSKV 228

Query: 153 KRAELWS 157
           K A+ W+
Sbjct: 229 KNAQFWT 235

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BLAST of Lsi03G007850 vs. NCBI nr
Match: gi|659111017|ref|XP_008455533.1| (PREDICTED: uncharacterized protein LOC103495684 [Cucumis melo])

HSP 1 Score: 241.9 bits (616), Expect = 7.6e-61
Identity = 136/162 (83.95%), Postives = 143/162 (88.27%), Query Frame = 1


Query: 1   MKKLGGFMCHSPAATAVCIPANPRSAVVSRRLARADDVNNA---NGNRARLVSNAVKYSK 60
           MKKLGGF CHS A TAVCIPA+PRSAVVSRRLARAD VNN    NGNRARLVSNAVKYSK
Sbjct: 1   MKKLGGFTCHSAATTAVCIPADPRSAVVSRRLARADYVNNNHANNGNRARLVSNAVKYSK 60

Query: 61  LVKCS-SLSVRSEGTGTVMKKP-VPIMLSSRPEN--VFHVVVMRVALHCQGCASKVKRHL 120
           LV+ S SLSVRSEG    +KKP +P+MLSS  EN  VFHVVVMRVALHCQGCASKVKRHL
Sbjct: 61  LVESSPSLSVRSEGKQINLKKPAMPVMLSSPAENNNVFHVVVMRVALHCQGCASKVKRHL 120

Query: 121 TKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELW 156
           +KMEGVTSFSIDLEAKRVTVMGHISPLGVL+SISKVKRAELW
Sbjct: 121 SKMEGVTSFSIDLEAKRVTVMGHISPLGVLQSISKVKRAELW 162

[Close]

BLAST of Lsi03G007850 vs. NCBI nr
Match: gi|778724303|ref|XP_011658781.1| (PREDICTED: uncharacterized protein LOC105436087 [Cucumis sativus])

HSP 1 Score: 224.9 bits (572), Expect = 9.7e-56
Identity = 130/165 (78.79%), Postives = 140/165 (84.85%), Query Frame = 1


Query: 1   MKKLGGFMCHSPAATAVCIPANPRSAVVSRRLARADDVNNA---NGNR-ARLVSN-AVKY 60
           MKKLGGFMCHS A TAVCIP++PRS VVSRRLAR D  NN    NGNR AR VSN  VKY
Sbjct: 1   MKKLGGFMCHSAATTAVCIPSDPRSTVVSRRLARPDYANNNHANNGNRSARFVSNNTVKY 60

Query: 61  SKLVKCS-SLSVRSEGTGTVMKKP-VPIMLSSRPEN--VFHVVVMRVALHCQGCASKVKR 120
           SKLV+ S SLSVRSEG    +KKP +P+MLSS  EN  VFHVVVMRVALHCQGCASKVKR
Sbjct: 61  SKLVESSPSLSVRSEGKHINLKKPAMPVMLSSPAENNNVFHVVVMRVALHCQGCASKVKR 120

Query: 121 HLTKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWS 157
           HL++MEGVTSFSIDLEAKRVTVMGHISPLGVL+SISKVKRAELW+
Sbjct: 121 HLSRMEGVTSFSIDLEAKRVTVMGHISPLGVLQSISKVKRAELWA 165

[Close]

BLAST of Lsi03G007850 vs. NCBI nr
Match: gi|731410542|ref|XP_010657601.1| (PREDICTED: uncharacterized protein LOC100243595 [Vitis vinifera])

HSP 1 Score: 163.7 bits (413), Expect = 2.6e-37
Identity = 98/167 (58.68%), Postives = 120/167 (71.86%), Query Frame = 1


Query: 2   KKLGGFMCHSPAATAVCIPANPRSAVVSRRLARADDVNNANGNRARLVSNAVKYSKLVKC 61
           KK+ G MCHSPAATAVCIP +PRS +V RR  R         + +RL++NA KYS+LV+ 
Sbjct: 7   KKMRGLMCHSPAATAVCIPGDPRSVIVPRRPDRT------LVDHSRLINNA-KYSRLVES 66

Query: 62  SSLSVRSEGTGTV--MKK-------PVPIML-SSRP--ENVFHVVVMRVALHCQGCASKV 121
              +  ++ +  V  MK+       P+P    SS P  ++VF VVVMRV+LHCQGCA KV
Sbjct: 67  HRFTPAAKRSVLVPAMKRGHRPSAEPMPFHPPSSTPSLDHVFQVVVMRVSLHCQGCAGKV 126

Query: 122 KRHLTKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWS 157
           K+HL+KMEGVTSFSIDLE KRVTVMGH+SP GVLESISKVK+AELWS
Sbjct: 127 KKHLSKMEGVTSFSIDLETKRVTVMGHVSPSGVLESISKVKKAELWS 166

[Close]

BLAST of Lsi03G007850 vs. NCBI nr
Match: gi|593792090|ref|XP_007159084.1| (hypothetical protein PHAVU_002G207200g [Phaseolus vulgaris])

HSP 1 Score: 156.0 bits (393), Expect = 5.5e-35
Identity = 88/158 (55.70%), Postives = 111/158 (70.25%), Query Frame = 1


Query: 6   GFMCHSPAATAVCIPA-NPRSAVVSRRLARADDVNNANGNRARLVSNAVKYSKLVK---- 65
           GFMCHS A+TAVC+   +PR  VV +RL R+  +++      RL+ N  KYSKLV+    
Sbjct: 14  GFMCHSQASTAVCMSTRDPRPVVVPKRLERSVFLDDT-----RLI-NYAKYSKLVEPPRS 73

Query: 66  --CSSLSVRSEGTGTVMKKPVPIMLSSRPENVFHVVVMRVALHCQGCASKVKRHLTKMEG 125
                + +R +       +P  ++ +    NVF VVVMRVA+HCQGCA KVK+HL+KMEG
Sbjct: 74  NPVPKIKLRGQDQDQAKNEPRELLKTQTDNNVFQVVVMRVAIHCQGCAGKVKKHLSKMEG 133

Query: 126 VTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWS 157
           VTSFSIDLE+KRVTVMGHISP+ VLESISKVKRAELW+
Sbjct: 134 VTSFSIDLESKRVTVMGHISPVAVLESISKVKRAELWT 165

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BLAST of Lsi03G007850 vs. NCBI nr
Match: gi|920687336|gb|KOM31319.1| (hypothetical protein LR48_Vigan01g087400 [Vigna angularis])

HSP 1 Score: 155.2 bits (391), Expect = 9.4e-35
Identity = 88/158 (55.70%), Postives = 111/158 (70.25%), Query Frame = 1


Query: 6   GFMCHSPAATAVCIPA-NPRSAVVSRRLARADDVNNANGNRARLVSNAVKYSKLVK---- 65
           GFMCHS A+TAVC+   +PRS V+ +RL R   +++      RL+ N  KYSKLV+    
Sbjct: 13  GFMCHSQASTAVCMSTRDPRSVVLPKRLERTVFLDDT-----RLI-NYAKYSKLVEPPRS 72

Query: 66  --CSSLSVRSEGTGTVMKKPVPIMLSSRPENVFHVVVMRVALHCQGCASKVKRHLTKMEG 125
                + +R +       +P  ++ +    NVF VVVMRVA+HCQGCA KVK+HL+KMEG
Sbjct: 73  NPVPKIKLRVQDQDQAKNQPKDLLKTQTDNNVFQVVVMRVAIHCQGCAVKVKKHLSKMEG 132

Query: 126 VTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWS 157
           VTSFSIDLE+KRVTVMGHISP+ VLESISKVKRAELW+
Sbjct: 133 VTSFSIDLESKRVTVMGHISPVAVLESISKVKRAELWT 164

The following BLAST results are available for this feature:

BLAST of Lsi03G007850 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ATOX1_ARATH	4.7e-10	47.62	Copper transport protein ATX1 OS=Arabidopsis thaliana GN=ATX1 PE=1 SV=2	[more]
CCH_ARATH	3.0e-09	46.03	Copper transport protein CCH OS=Arabidopsis thaliana GN=CCH PE=1 SV=1	[more]
HIP20_ARATH	2.4e-06	35.21	Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana GN...	[more]

BLAST of Lsi03G007850 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K2I2_CUCSA	6.7e-56	78.79	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G060180 PE=4 SV=1	[more]
V7CNZ1_PHAVU	3.8e-35	55.70	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G207200g PE=4 SV=1	[more]
A0A0L9TMD1_PHAAN	6.6e-35	55.70	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g087400 PE=4 SV=1	[more]
A0A0S3R048_PHAAN	6.6e-35	55.70	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G156700 PE=...	[more]
F6H1N1_VITVI	3.3e-34	54.39	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0055g00930 PE=4 SV=...	[more]

BLAST of Lsi03G007850 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G28660.1	1.1e-20	50.00	Chloroplast-targeted copper chaperone protein	[more]
AT5G02600.2	5.9e-19	65.08	Heavy metal transport/detoxification superfamily protein	[more]
AT2G37390.1	1.7e-18	60.94	Chloroplast-targeted copper chaperone protein	[more]
AT3G24450.1	1.4e-17	44.09	Heavy metal transport/detoxification superfamily protein	[more]
AT3G53530.2	3.0e-15	59.70	Chloroplast-targeted copper chaperone protein	[more]

BLAST of Lsi03G007850 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659111017\|ref\|XP_008455533.1\|	7.6e-61	83.95	PREDICTED: uncharacterized protein LOC103495684 [Cucumis melo]	[more]
gi\|778724303\|ref\|XP_011658781.1\|	9.7e-56	78.79	PREDICTED: uncharacterized protein LOC105436087 [Cucumis sativus]	[more]
gi\|731410542\|ref\|XP_010657601.1\|	2.6e-37	58.68	PREDICTED: uncharacterized protein LOC100243595 [Vitis vinifera]	[more]
gi\|593792090\|ref\|XP_007159084.1\|	5.5e-35	55.70	hypothetical protein PHAVU_002G207200g [Phaseolus vulgaris]	[more]
gi\|920687336\|gb\|KOM31319.1\|	9.4e-35	55.70	hypothetical protein LR48_Vigan01g087400 [Vigna angularis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0046872	metal ion binding

Vocabulary: **Biological Process**
Term	Definition
GO:0030001	metal ion transport

Vocabulary: **INTERPRO**
Term	Definition
IPR006121	HMA_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046916	cellular transition metal ion homeostasis
biological_process	GO:0030001	metal ion transport
biological_process	GO:0008150	biological_process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046914	transition metal ion binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G007850.1	Lsi03G007850.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 96..144 score: 1.2
IPR006121	Heavy metal-associated domain, HMA	PROFILE	PS50846	HMA_2	coord: 92..149 score: 11
IPR006121	Heavy metal-associated domain, HMA	unknown	SSF55008	HMA, heavy metal-associated domain	coord: 92..150 score: 9.69
None	No IPR available	GENE3D	G3DSA:3.30.70.100		coord: 93..155 score: 6.0
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 79..155 score: 1.5
None	No IPR available	PANTHER	PTHR22814:SF82	SUBFAMILY NOT NAMED	coord: 79..155 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G007850	Csa7G060180	Cucumber (Chinese Long) v2	culsiB522
Lsi03G007850	CSPI07G05370	Wild cucumber (PI 183967)	cpilsiB536
Lsi03G007850	Cucsa.217770	Cucumber (Gy14) v1	cgylsiB367
Lsi03G007850	CmaCh19G002710	Cucurbita maxima (Rimu)	cmalsiB482
Lsi03G007850	CmoCh19G002960	Cucurbita moschata (Rifu)	cmolsiB466
Lsi03G007850	Cla006387	Watermelon (97103) v1	lsiwmB235
Lsi03G007850	MELO3C018661	Melon (DHL92) v3.5.1	lsimeB247
Lsi03G007850	ClCG09G015140	Watermelon (Charleston Gray)	lsiwcgB215
Lsi03G007850	MELO3C018661.2	Melon (DHL92) v3.6.1	lsimedB260
Lsi03G007850	Cla97C09G177230	Watermelon (97103) v2	lsiwmbB251
Lsi03G007850	Bhi05G000669	Wax gourd	lsiwgoB320
Lsi03G007850	Cp4.1LG15g02430	Cucurbita pepo (Zucchini)	cpelsiB206
Lsi03G007850	CsGy7G004970	Cucumber (Gy14) v2	cgyblsiB489
Lsi03G007850	Carg24572	Silver-seed gourd	carlsiB590
Lsi03G007850	CsaV3_7G006310	Cucumber (Chinese Long) v3	cuclsiB555

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G007850	Cucurbita maxima (Rimu)	cmalsiB117
Lsi03G007850	Cucurbita moschata (Rifu)	cmolsiB104
Lsi03G007850	Cucurbita pepo (Zucchini)	cpelsiB544
Lsi03G007850	Silver-seed gourd	carlsiB674