Lsi02G002790 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi02G002790
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionKunitz trypsin inhibitor
Locationchr02 : 2360483 .. 2361100 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAGCTTCGGAATATTACTGTCTTTTCTTTTCATTCTCCTTGCCTCTACCGAGGTTCGCTTCTCCAGAGCCGAAGCTTCGCCGGAGGCCGTCCGCGATATCGACGGCAATAAGCTCCGAGCCGGTGTCAACTATTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGAAATGTCCACTGAACGTGGTTCAAGAACAATTCGAATTAATGAACGGCTTGCCTACAACATTTGCGCCTGTAAACCCTAAAAAAGGAGTGATTCGAGTTTCGACGGATTTGAATGTGCAATTCGAGGCGAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGGACGGTAAGGATTATAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGCAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

mRNA sequence

ATGAAGAGCTTCGGAATATTACTGTCTTTTCTTTTCATTCTCCTTGCCTCTACCGAGGTTCGCTTCTCCAGAGCCGAAGCTTCGCCGGAGGCCGTCCGCGATATCGACGGCAATAAGCTCCGAGCCGGTGTCAACTATTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGAAATGTCCACTGAACGTGGTTCAAGAACAATTCGAATTAATGAACGGCTTGCCTACAACATTTGCGCCTGTAAACCCTAAAAAAGGAGTGATTCGAGTTTCGACGGATTTGAATGTGCAATTCGAGGCGAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGGACGGTAAGGATTATAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGCAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

Coding sequence (CDS)

ATGAAGAGCTTCGGAATATTACTGTCTTTTCTTTTCATTCTCCTTGCCTCTACCGAGGTTCGCTTCTCCAGAGCCGAAGCTTCGCCGGAGGCCGTCCGCGATATCGACGGCAATAAGCTCCGAGCCGGTGTCAACTATTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGAAATGTCCACTGAACGTGGTTCAAGAACAATTCGAATTAATGAACGGCTTGCCTACAACATTTGCGCCTGTAAACCCTAAAAAAGGAGTGATTCGAGTTTCGACGGATTTGAATGTGCAATTCGAGGCGAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGGACGGTAAGGATTATAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGCAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

Protein sequence

MKSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDIGIFFKNGKRALALSDTPFPVMFKKV
Homology
[Close]
BLAST of Lsi02G002790 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 209.1 bits (531), Expect = 4.4e-53
Identity = 113/216 (52.31%), Postives = 140/216 (64.81%), Query Frame = 1 

Query: 1   MKSFGIL-LSFLFI---LLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRG 60
           MK   +L LSF F+   L A+     S A+++P  V DIDG KLR G NYYI+PV R  G
Sbjct: 1   MKELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHG 60

Query: 61  GGLTLGNLQSEK---CPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTI 120
           GGLT+          CP  VVQ + E+ +  P  F P NPK+ V+RVSTDLN+ F A   
Sbjct: 61  GGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 120

Query: 121 C--ATSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEK--DGKDYKLVFCPSV 180
           C   +STVW+LDK+DESTGQ+FVTIGG +GNPG ET+ +WFKIE+      YKLVFCP+V
Sbjct: 121 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTV 180

Query: 181 CDFCKVMCRDIGIFF-KNGKRALALSDTPFPVMFKK 205
           C  CKV C D+GI+  + G+R LALSD PF   F K
Sbjct: 181 CGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]
BLAST of Lsi02G002790 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 3.5e-42
Identity = 94/204 (46.08%), Postives = 122/204 (59.80%), Query Frame = 1 

Query: 6   ILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGG-LTLGNL 65
           +LL F F    S    F  A A+   V D DG++L+ GV YY+L    G GGG L LG  
Sbjct: 8   VLLLFAF---TSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRA 67

Query: 66  QSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEA--STICATSTVWKL 125
             + CP  VVQ + +L NG P  F+  + K  V+RVSTD+N++F      +C+TSTVW+L
Sbjct: 68  TGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRL 127

Query: 126 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDG-KDYKLVFCPSVCDFCKVMCRDIG 185
           D +D S G+W+VT  G +G PG  T+ +WFKIEK G   YK  FCPSVCD C  +C DIG
Sbjct: 128 DNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIG 187

Query: 186 IFF-KNGKRALALSDTPFPVMFKK 205
                +G+  LALSD  +  MFKK
Sbjct: 188 RHSDDDGQIRLALSDNEWAWMFKK 208

[Close]
BLAST of Lsi02G002790 vs. Swiss-Prot
Match: DRTI_DELRE (Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.5e-24
Identity = 71/180 (39.44%), Postives = 98/180 (54.44%), Query Frame = 1 

Query: 30  EAVRDIDGNKLRAGVNYYILPVFRGR-GGGLTLGNLQSEKCPLNVVQEQFELMNGLPTTF 89
           E V DI+G  +  G  YYI+    G  GGG+  G  +   CP++++QEQ +L  GLP  F
Sbjct: 4   EKVYDIEGYPVFLGSEYYIVSAIIGAGGGGVRPGRTRGSMCPMSIIQEQSDLQMGLPVRF 63

Query: 90  APVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPGVETV 149
           +     +G I   T+L ++F     CA S+ W + K    +G+  V IGGS  +P  E V
Sbjct: 64  SSPEESQGKIYTDTELEIEFVEKPDCAESSKWVIVK---DSGEARVAIGGSEDHPQGELV 123

Query: 150 DNWFKIEKDGK-DYKLVFCPSVCDFCKVMCRDIGIFFKNGKRALAL---SDTPFPVMFKK 205
             +FKIEK G   YKLVFCP         C DIGI ++ G+R+L L    D+PF V+F K
Sbjct: 124 RGFFKIEKLGSLAYKLVFCPK---SSSGSCSDIGINYE-GRRSLVLKSSDDSPFRVVFVK 176

[Close]
BLAST of Lsi02G002790 vs. Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 7.4e-24
Identity = 70/184 (38.04%), Postives = 95/184 (51.63%), Query Frame = 1 

Query: 29  PEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQFELMNGLPTTF 88
           P  V D DGN+LRA  NYY+LP  R  GGGLT+      +CPL V QE     +GLP   
Sbjct: 2   PPPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQEADGQRDGLPVRI 61

Query: 89  APVN--PKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPGVE 148
           AP    P   +IR+STD+ + F A T C  ST W +D  +  +G+  V  G  R +P   
Sbjct: 62  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHIDS-ELVSGRRHVITGPVR-DPSPS 121

Query: 149 TVDNWFKIEK----DGKDYKLVFCPSVCDFCKVMCRDIGIF--FKNGKRALALSDTPFPV 205
             +N F+IEK    +  +YKL+ C          C+D+G+F   K G   L  ++    V
Sbjct: 122 GRENAFRIEKYSGAEVHEYKLMACGD-------SCQDLGVFRDLKGGAWFLGATEPYHVV 176

[Close]
BLAST of Lsi02G002790 vs. Swiss-Prot
Match: PIP21_PEA (Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV=2)

HSP 1 Score: 108.2 bits (269), Expect = 1.1e-22
Identity = 70/198 (35.35%), Postives = 98/198 (49.49%), Query Frame = 1 

Query: 10  FLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRG-RGGGLTLGNLQSEK 69
           FLF+ + +  + FS  +   E V D +GN +  GV Y+ILP  RG  GGG+ LG      
Sbjct: 12  FLFVFITNLSLAFSNEDV--EQVLDFNGNPIFPGVQYFILPAIRGPAGGGVRLGRTGDLT 71

Query: 70  CPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDES 129
           CP+ V+Q++ E+ NGLP  F       G+I   T + +++     CA S+ W L   D  
Sbjct: 72  CPVTVLQDRQEVKNGLPVKFVIPEISPGIIFTGTPIEIEYTKKPNCAKSSKW-LVFVDNV 131

Query: 130 TGQWFVTIGGSRGNPGVETVDNWFKIEK--DGKDYKLVFCPSVCDFCKVMCRDIGIF--F 189
             +  V IGG    PGV+T+   FKIEK   G  YKL FC          C D+G F   
Sbjct: 132 IQKACVGIGGPENYPGVQTLSGLFKIEKHESGFGYKLGFCVK----GSPTCLDVGRFDND 191

Query: 190 KNGKRALALSDTPFPVMF 203
           ++G+R        F V+F
Sbjct: 192 EDGRRLNLTEHESFQVVF 202

[Close]
BLAST of Lsi02G002790 vs. TrEMBL
Match: A0A0A0LTW2_CUCSA (Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 6.6e-104
Identity = 185/206 (89.81%), Postives = 194/206 (94.17%), Query Frame = 1 

Query: 1   MKSFGILLSFLFILLASTE-VRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGL 60
           MK+FGIL  FLFILLAST+ +RFS A+ASPEAV DIDG KLRAGVNYYILPVFRGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           TLGNLQSEKCPLNVVQEQ E+MNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRD 180
           WKLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEK GKDYKLVFCP+VC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           IGIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

[Close]
BLAST of Lsi02G002790 vs. TrEMBL
Match: A0A0A0LT81_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.2e-75
Identity = 129/206 (62.62%), Postives = 171/206 (83.01%), Query Frame = 1 

Query: 1   MKSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLT 60
           M++F  LL FLFI++AS+EVRF RA+ASP+AV D DG KLRAG  YYIL V+    GGL+
Sbjct: 1   MRNFA-LLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLS 60

Query: 61  LGNLQS-EKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           +G +   EKCP+N++ E ++ ++GLP TF+P+NPKKGV+RVSTDLN+QFEA+T C  STV
Sbjct: 61  IGGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRD 180
           WK+ KFDE   Q+FVT+GG +GNPG ET++NWFK+EK GK+YKLV+CP+VC +CKV+C+D
Sbjct: 121 WKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           +G+F+KNG+R +AL+D PFPVMFKKV
Sbjct: 181 VGLFYKNGRRVIALNDAPFPVMFKKV 205

[Close]
BLAST of Lsi02G002790 vs. TrEMBL
Match: A5AXN3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027379 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 3.9e-72
Identity = 136/204 (66.67%), Postives = 160/204 (78.43%), Query Frame = 1 

Query: 1   MKSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLT 60
           MK+  +L S L I LA      + AE++P+ V D +G KLR+GV+YYILPVFRGRGGGLT
Sbjct: 1   MKTTSLLFSLLLIALAVKPFSVA-AESAPDPVLDTEGKKLRSGVDYYILPVFRGRGGGLT 60

Query: 61  LGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 120
           L +  +E CPL+VVQEQ E+ NGLP TF PVNPKKGVIRVSTD N++F ASTIC  ST+W
Sbjct: 61  LASTGNESCPLDVVQEQQEVSNGLPLTFTPVNPKKGVIRVSTDHNIKFSASTICVQSTLW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDI 180
           KL+ +DES+GQWFVT GG  GNPG ET+DNWFKIEK   DYKLVFCP+VCDFCK +C DI
Sbjct: 121 KLE-YDESSGQWFVTTGGVEGNPGRETLDNWFKIEKYEDDYKLVFCPTVCDFCKPVCGDI 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKK 205
           GI+ +NG R LALSD PF VMFKK
Sbjct: 181 GIYIQNGYRRLALSDVPFKVMFKK 202

[Close]
BLAST of Lsi02G002790 vs. TrEMBL
Match: M5X024_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011496mg PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 1.9e-71
Identity = 133/200 (66.50%), Postives = 157/200 (78.50%), Query Frame = 1 

Query: 8   LSFLFILLA-STEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTLGNLQS 67
           L F F+L A S ++R   A+A+P  V DI GNKL+ GV+YYILPV RGRGGGLTL +  +
Sbjct: 9   LVFCFLLFAFSAKLRSVAADAAPSPVLDITGNKLQTGVDYYILPVIRGRGGGLTLASTSN 68

Query: 68  E-KCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKF 127
           +  CPL+VVQEQ E+ NGLP  F+PVN  KGV+RVSTDLN++F A+TIC  STVWKL KF
Sbjct: 69  KTSCPLDVVQEQNEVSNGLPLKFSPVNVTKGVVRVSTDLNIKFSATTICVQSTVWKLGKF 128

Query: 128 DESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDIGIFFK 187
           DE TGQWFVT GG  GNPG +T  NWFKIEK G DYKLVFCP+VC+FCKV+C D+GIFF+
Sbjct: 129 DEQTGQWFVTSGGVEGNPGRQTTSNWFKIEKFGDDYKLVFCPTVCNFCKVICGDVGIFFQ 188

Query: 188 NGKRALALSDTPFPVMFKKV 206
           +GKR LALSD PF  MFKKV
Sbjct: 189 DGKRRLALSDVPFRAMFKKV 208

[Close]
BLAST of Lsi02G002790 vs. TrEMBL
Match: A0A061EZK2_THECC (Kunitz family trypsin and protease inhibitor protein OS=Theobroma cacao GN=TCM_025233 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 7.3e-71
Identity = 124/180 (68.89%), Postives = 144/180 (80.00%), Query Frame = 1 

Query: 25  AEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQFELMNGL 84
           A A+P+ V DI G KLR G +YYILPVFRGRGGGLTL +  +E CPL+VVQEQ E+ +GL
Sbjct: 15  ANAAPDPVLDISGKKLRTGTDYYILPVFRGRGGGLTLASTGNESCPLDVVQEQLEVSDGL 74

Query: 85  PTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPG 144
           P TF+PVN KKGV+RVSTD N++F A+TIC   T+WKLD FD+ST QWFVT GG  GNPG
Sbjct: 75  PVTFSPVNIKKGVVRVSTDQNIKFSAATICVQPTLWKLDSFDDSTRQWFVTTGGVEGNPG 134

Query: 145 VETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDIGIFFKNGKRALALSDTPFPVMFKK 204
            ET+DNWFKIEK   DYKLVFCP+VCDFCKVMCRD+G+F   G R LALSD PF VMFK+
Sbjct: 135 RETIDNWFKIEKYEDDYKLVFCPTVCDFCKVMCRDVGVFIDGGVRRLALSDVPFKVMFKR 194

[Close]
BLAST of Lsi02G002790 vs. TAIR10
Match: AT1G17860.1 (AT1G17860.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 228.0 bits (580), Expect = 5.2e-60
Identity = 109/198 (55.05%), Postives = 144/198 (72.73%), Query Frame = 1 

Query: 8   LSFLFILLAS-TEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTLGNLQS 67
           L ++F+LLA     R    EA+ E V+DI+G  L  GVNYYILPV RGRGGGLT+ NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 68  EKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 127
           E CP +V+Q+QFE+  GLP  F+P + K   I VSTD+N++F  ++I      W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPTSI------WELANFD 123

Query: 128 ESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDIGIFFKN 187
           E+T QWF++  G  GNPG +TVDNWFKI+K  KDYK+ FCP+VC+FCKV+CRD+G+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 188 GKRALALSDTPFPVMFKK 205
           GKR LALSD P  VMFK+
Sbjct: 184 GKRRLALSDVPLKVMFKR 194

[Close]
BLAST of Lsi02G002790 vs. TAIR10
Match: AT1G73260.1 (AT1G73260.1 kunitz trypsin inhibitor 1)

HSP 1 Score: 154.5 bits (389), Expect = 7.3e-38
Identity = 87/196 (44.39%), Postives = 116/196 (59.18%), Query Frame = 1 

Query: 12  FILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPL 71
           +++LA T V  S A     AV DIDGN +    +YY+LPV RGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFE-ASTICATSTVWKLDKFDESTG 131
           ++VQE  E+  G+P  F+    K   +  S +LN++ +  +TIC  ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKDGKD-YKLVFCPSVCDFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G+D YK VFCP  CD     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]
BLAST of Lsi02G002790 vs. TAIR10
Match: AT1G73325.1 (AT1G73325.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 107.5 bits (267), Expect = 1.0e-23
Identity = 73/208 (35.10%), Postives = 120/208 (57.69%), Query Frame = 1 

Query: 6   ILLSFLFI-LLASTEVRFSRAEASP-EAVRDIDGNKLRAGVNYYILPVFRGRGGGLTLGN 65
           + LSF+ + +L++     S A+A+P + V DI G+ +++ V YYI+P   G GGGL   N
Sbjct: 4   LTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSN 63

Query: 66  --LQSEKCPLN--VVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEAST-ICATST 125
             L ++   LN  +VQ     ++GLP TF+P+N K   +++S  LN++F+++  +C  S 
Sbjct: 64  RNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSK 123

Query: 126 VWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCR 185
           VW++D       + FV+IGG +G       ++WF+I++DG  YKL++CP       V C 
Sbjct: 124 VWRID-HSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIVACI 183

Query: 186 DIGI-FFKNGKRALALS-DTPFPVMFKK 205
           ++ +    +G R L LS D  F V F+K
Sbjct: 184 NVSLEIDDHGVRRLVLSTDQSFVVKFQK 202

[Close]
BLAST of Lsi02G002790 vs. TAIR10
Match: AT3G04320.1 (AT3G04320.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 48.9 bits (115), Expect = 4.3e-06
Identity = 44/170 (25.88%), Postives = 72/170 (42.35%), Query Frame = 1 

Query: 1   MKSFGILL-SFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGL 60
           MK+F +++ SFLF+ + +T    S      + V D +G+ ++  V YYI  +       +
Sbjct: 1   MKTFQLMMISFLFVAITTTSGVVSEGN---DVVYDGEGDPVKPNVPYYISFMTSDYNMWI 60

Query: 61  T---LGNLQSEKCPLNVVQEQF-ELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICA 120
                G+     CP   +      L    P  F   N K  V+R S  L ++F     C 
Sbjct: 61  CRMQYGSTDPNSCPQQPLMVTHPNLAAPTPVMFVLAN-KSDVVRESAKLKIKFVGPRQCG 120

Query: 121 TSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVF 166
            S  WK+ + + S G+ F+      G+  +   D+ F I K  + YK  F
Sbjct: 121 KSGFWKVVQRNSSEGEVFL-----NGSKSMSHNDSTFAIHKTNEYYKFTF 161

[Close]
BLAST of Lsi02G002790 vs. NCBI nr
Match: gi|449439521|ref|XP_004137534.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 384.8 bits (987), Expect = 9.4e-104
Identity = 185/206 (89.81%), Postives = 194/206 (94.17%), Query Frame = 1 

Query: 1   MKSFGILLSFLFILLASTE-VRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGL 60
           MK+FGIL  FLFILLAST+ +RFS A+ASPEAV DIDG KLRAGVNYYILPVFRGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           TLGNLQSEKCPLNVVQEQ E+MNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRD 180
           WKLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEK GKDYKLVFCP+VC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           IGIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

[Close]
BLAST of Lsi02G002790 vs. NCBI nr
Match: gi|659066983|ref|XP_008437058.1| (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 383.3 bits (983), Expect = 2.7e-103
Identity = 184/204 (90.20%), Postives = 193/204 (94.61%), Query Frame = 1 

Query: 2   KSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTL 61
           K+FGI   F+FILLASTE+RFS A+ASPEAV DIDG KLRAGVNYYILPVFRGRGGGLTL
Sbjct: 3   KNFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTL 62

Query: 62  GNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWK 121
           GNLQSE CP+NVVQEQFELMNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWK
Sbjct: 63  GNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122

Query: 122 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDIG 181
           LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEK GKDYKLVFCP+VC+FCKVMCRDIG
Sbjct: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182

Query: 182 IFFKNGKRALALSDTPFPVMFKKV 206
           IFFKNGKRALALSDTPFPVMFKKV
Sbjct: 183 IFFKNGKRALALSDTPFPVMFKKV 205

[Close]
BLAST of Lsi02G002790 vs. NCBI nr
Match: gi|449439731|ref|XP_004137639.1| (PREDICTED: miraculin [Cucumis sativus])

HSP 1 Score: 290.0 bits (741), Expect = 3.2e-75
Identity = 129/206 (62.62%), Postives = 171/206 (83.01%), Query Frame = 1 

Query: 1   MKSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLT 60
           M++F  LL FLFI++AS+EVRF RA+ASP+AV D DG KLRAG  YYIL V+    GGL+
Sbjct: 1   MRNFA-LLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLS 60

Query: 61  LGNLQS-EKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           +G +   EKCP+N++ E ++ ++GLP TF+P+NPKKGV+RVSTDLN+QFEA+T C  STV
Sbjct: 61  IGGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRD 180
           WK+ KFDE   Q+FVT+GG +GNPG ET++NWFK+EK GK+YKLV+CP+VC +CKV+C+D
Sbjct: 121 WKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           +G+F+KNG+R +AL+D PFPVMFKKV
Sbjct: 181 VGLFYKNGRRVIALNDAPFPVMFKKV 205

[Close]
BLAST of Lsi02G002790 vs. NCBI nr
Match: gi|659066987|ref|XP_008437082.1| (PREDICTED: miraculin [Cucumis melo])

HSP 1 Score: 289.7 bits (740), Expect = 4.1e-75
Identity = 130/206 (63.11%), Postives = 169/206 (82.04%), Query Frame = 1 

Query: 1   MKSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLT 60
           MK+F  LL FLFI++AS+EVRF RA+ASP+AV D DG KLRAG  YYIL V+    GGL+
Sbjct: 1   MKNFA-LLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLS 60

Query: 61  LGNLQS-EKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           +G +   EKCP+N++ E ++ ++GLP TF+PVNPKKGV+RVSTDLN++FEAST C  STV
Sbjct: 61  IGGIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRD 180
           WK+ KFD+   Q+FVT+GG++GNPG ET+ NWFK+EK GK+YK V+CP+VC +CKVMC+D
Sbjct: 121 WKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           +G+F+KNG+R  AL+D PFPVMFKKV
Sbjct: 181 VGLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]
BLAST of Lsi02G002790 vs. NCBI nr
Match: gi|147805678|emb|CAN65022.1| (hypothetical protein VITISV_027379 [Vitis vinifera])

HSP 1 Score: 279.3 bits (713), Expect = 5.6e-72
Identity = 136/204 (66.67%), Postives = 160/204 (78.43%), Query Frame = 1 

Query: 1   MKSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLT 60
           MK+  +L S L I LA      + AE++P+ V D +G KLR+GV+YYILPVFRGRGGGLT
Sbjct: 1   MKTTSLLFSLLLIALAVKPFSVA-AESAPDPVLDTEGKKLRSGVDYYILPVFRGRGGGLT 60

Query: 61  LGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 120
           L +  +E CPL+VVQEQ E+ NGLP TF PVNPKKGVIRVSTD N++F ASTIC  ST+W
Sbjct: 61  LASTGNESCPLDVVQEQQEVSNGLPLTFTPVNPKKGVIRVSTDHNIKFSASTICVQSTLW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDI 180
           KL+ +DES+GQWFVT GG  GNPG ET+DNWFKIEK   DYKLVFCP+VCDFCK +C DI
Sbjct: 121 KLE-YDESSGQWFVTTGGVEGNPGRETLDNWFKIEKYEDDYKLVFCPTVCDFCKPVCGDI 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKK 205
           GI+ +NG R LALSD PF VMFKK
Sbjct: 181 GIYIQNGYRRLALSDVPFKVMFKK 202

The following BLAST results are available for this feature:
BLAST of Lsi02G002790 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MIRA_SYNDU4.4e-5352.31Miraculin OS=Synsepalum dulcificum PE=1 SV=3[more]
ASP_THECC3.5e-4246.0821 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1[more]
DRTI_DELRE1.5e-2439.44Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1[more]
IAAS_WHEAT7.4e-2438.04Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1[more]
PIP21_PEA1.1e-2235.35Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV... [more]
BLAST of Lsi02G002790 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LTW2_CUCSA6.6e-10489.81Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1[more]
A0A0A0LT81_CUCSA2.2e-7562.62Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1[more]
A5AXN3_VITVI3.9e-7266.67Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027379 PE=4 SV=1[more]
M5X024_PRUPE1.9e-7166.50Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011496mg PE=4 SV=1[more]
A0A061EZK2_THECC7.3e-7168.89Kunitz family trypsin and protease inhibitor protein OS=Theobroma cacao GN=TCM_0... [more]
BLAST of Lsi02G002790 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G17860.15.2e-6055.05 Kunitz family trypsin and protease inhibitor protein[more]
AT1G73260.17.3e-3844.39 kunitz trypsin inhibitor 1[more]
AT1G73325.11.0e-2335.10 Kunitz family trypsin and protease inhibitor protein[more]
AT3G04320.14.3e-0625.88 Kunitz family trypsin and protease inhibitor protein[more]
BLAST of Lsi02G002790 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449439521|ref|XP_004137534.1|9.4e-10489.81PREDICTED: miraculin-like [Cucumis sativus][more]
gi|659066983|ref|XP_008437058.1|2.7e-10390.20PREDICTED: miraculin-like [Cucumis melo][more]
gi|449439731|ref|XP_004137639.1|3.2e-7562.62PREDICTED: miraculin [Cucumis sativus][more]
gi|659066987|ref|XP_008437082.1|4.1e-7563.11PREDICTED: miraculin [Cucumis melo][more]
gi|147805678|emb|CAN65022.1|5.6e-7266.67hypothetical protein VITISV_027379 [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004866endopeptidase inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR011065Kunitz_inhibitor_STI-like_sf
IPR002160Prot_inh_Kunz-lg
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004866 endopeptidase inhibitor activity
molecular_function GO:0008233 peptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G002790.1Lsi02G002790.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumePRINTSPR00291KUNITZINHBTRcoord: 31..60
score: 7.3E-18coord: 69..89
score: 7.3E-18coord: 174..203
score: 7.3E-18coord: 151..170
score: 7.3
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 32..204
score: 5.9
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 31..205
score: 7.7
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 32..48
scor
IPR011065Kunitz inhibitor ST1-likeunknownSSF50386STI-likecoord: 28..205
score: 1.72
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 28..205
score: 5.0
NoneNo IPR availablePANTHERPTHR33107FAMILY NOT NAMEDcoord: 1..205
score: 1.7