Cla97C01G013760 (gene) Watermelon (97103) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla97C01G013760
Type	gene
Organism	Citrullus lanatus (Watermelon (97103) v2)
Description	Kunitz trypsin inhibitor
Location	Cla97Chr01 : 27592221 .. 27592838 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAATTTGGGAATATTACTTTCTTTTCTTTTCATTCTCCTTGCCTCTACCGTGGTCCGCTTCTCCAGAGCCGACGCTTCGCCGGAGGCCGTCCGCGACATCGACGGCGATAAGCTCCGAGCCGGCGTCAATTATTACATCCTCCCTGTTATCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGACATGTCCACTCAACGTGGTTCAAGAACAATTCGAAGTGATGAACGGCTTGCCTACAACATTTGCGCCTGTAAACCCTAAAAAGGGAGTGATTCGAGTTTCGACGGATTTGAACGTGCAATTCGAGGCAAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTCGGGATTACAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCAGTTATGTTCAAGAAAGTTTGA

mRNA sequence

ATGAAGAATTTGGGAATATTACTTTCTTTTCTTTTCATTCTCCTTGCCTCTACCGTGGTCCGCTTCTCCAGAGCCGACGCTTCGCCGGAGGCCGTCCGCGACATCGACGGCGATAAGCTCCGAGCCGGCGTCAATTATTACATCCTCCCTGTTATCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGACATGTCCACTCAACGTGGTTCAAGAACAATTCGAAGTGATGAACGGCTTGCCTACAACATTTGCGCCTGTAAACCCTAAAAAGGGAGTGATTCGAGTTTCGACGGATTTGAACGTGCAATTCGAGGCAAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTCGGGATTACAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCAGTTATGTTCAAGAAAGTTTGA

Coding sequence (CDS)

ATGAAGAATTTGGGAATATTACTTTCTTTTCTTTTCATTCTCCTTGCCTCTACCGTGGTCCGCTTCTCCAGAGCCGACGCTTCGCCGGAGGCCGTCCGCGACATCGACGGCGATAAGCTCCGAGCCGGCGTCAATTATTACATCCTCCCTGTTATCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGACATGTCCACTCAACGTGGTTCAAGAACAATTCGAAGTGATGAACGGCTTGCCTACAACATTTGCGCCTGTAAACCCTAAAAAGGGAGTGATTCGAGTTTCGACGGATTTGAACGTGCAATTCGAGGCAAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTCGGGATTACAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCAGTTATGTTCAAGAAAGTTTGA

Protein sequence

MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDIGIFFKNGKRALALSDTPFPVMFKKV

Homology

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BLAST of Cla97C01G013760 vs. NCBI nr
Match: XP_004137534.1 (PREDICTED: miraculin-like [Cucumis sativus] >KGN64217.1 Tumor-related protein [Cucumis sativus])

HSP 1 Score: 383.6 bits (984), Expect = 4.1e-103
Identity = 185/206 (89.81%), Postives = 192/206 (93.20%), Query Frame = 0


Query: 1   MKNLGILLSFLFILLAST-VVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGL 60
           MKN GIL  FLFILLAST ++RFS ADASPEAV DIDG KLRAGVNYYILPV RGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           TLGNLQSE CPLNVVQEQ EVMNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRD 180
           WKLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           IGIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

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BLAST of Cla97C01G013760 vs. NCBI nr
Match: XP_008437058.1 (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 380.9 bits (977), Expect = 2.6e-102
Identity = 182/204 (89.22%), Postives = 191/204 (93.63%), Query Frame = 0


Query: 2   KNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTL 61
           KN GI   F+FILLAST +RFS ADASPEAV DIDG KLRAGVNYYILPV RGRGGGLTL
Sbjct: 3   KNFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTL 62

Query: 62  GNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWK 121
           GNLQSE CP+NVVQEQFE+MNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWK
Sbjct: 63  GNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122

Query: 122 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDIG 181
           LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRDIG
Sbjct: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182

Query: 182 IFFKNGKRALALSDTPFPVMFKKV 206
           IFFKNGKRALALSDTPFPVMFKKV
Sbjct: 183 IFFKNGKRALALSDTPFPVMFKKV 205

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BLAST of Cla97C01G013760 vs. NCBI nr
Match: XP_022923703.1 (kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin inhibitor 2 [Cucurbita maxima])

HSP 1 Score: 369.4 bits (947), Expect = 8.0e-99
Identity = 178/205 (86.83%), Postives = 188/205 (91.71%), Query Frame = 0


Query: 1   MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 60
           MKN   LLSFLFILLAS  VR SRADASP+AVRDIDG KLRAGVNYYILPV RGRGGGL 
Sbjct: 1   MKNFA-LLSFLFILLASE-VRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLA 60

Query: 61  LGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 120
           LGNLQS+ CPLNVVQEQFE+MNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDI 180
           KLDKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCP+VCDFCKVMCRD+
Sbjct: 121 KLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDV 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFK+V
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKEV 203

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BLAST of Cla97C01G013760 vs. NCBI nr
Match: XP_023520252.1 (kunitz trypsin inhibitor 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 368.2 bits (944), Expect = 1.8e-98
Identity = 178/205 (86.83%), Postives = 187/205 (91.22%), Query Frame = 0


Query: 1   MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 60
           MKN   LLSFLFILLAS  VR SRADASP+AVRDIDG KLRAGVNYYILPV RGRGGGL 
Sbjct: 1   MKNFA-LLSFLFILLASE-VRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLA 60

Query: 61  LGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 120
           LGNLQS+ CPLNVVQEQFE+MNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDI 180
           KLDKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCP+VCDFCKVMCRD+
Sbjct: 121 KLDKFDESTKQWFITIGGARGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDV 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFK V
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKVV 203

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BLAST of Cla97C01G013760 vs. NCBI nr
Match: XP_022137472.1 (kunitz trypsin inhibitor 2 [Momordica charantia])

HSP 1 Score: 347.8 bits (891), Expect = 2.5e-92
Identity = 164/205 (80.00%), Postives = 180/205 (87.80%), Query Frame = 0


Query: 1   MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 60
           MKN   LL FLF L+AST VR  ++DASP+AV DIDG KLRAGVNYYILPVIRGRGGGLT
Sbjct: 1   MKNFA-LLCFLFALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLT 60

Query: 61  LGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 120
           LGN+  + CPL+VVQEQFE+ NGLP TFAPVNPKKGV+RVSTDLNV+FEAST+CA STVW
Sbjct: 61  LGNIGHDKCPLHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAKSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDI 180
           KLDKFDEST QW V IGG RGNPG ET+DNWFKIEKHG+DYK VFCPSVC FCKV+C+D+
Sbjct: 121 KLDKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGKDYKFVFCPSVCKFCKVICKDV 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIF KNG RALALSDTPFPVMFKKV
Sbjct: 181 GIFIKNGNRALALSDTPFPVMFKKV 204

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BLAST of Cla97C01G013760 vs. TrEMBL
Match: tr|A0A0A0LTW2|A0A0A0LTW2_CUCSA (Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.7e-103
Identity = 185/206 (89.81%), Postives = 192/206 (93.20%), Query Frame = 0


Query: 1   MKNLGILLSFLFILLAST-VVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGL 60
           MKN GIL  FLFILLAST ++RFS ADASPEAV DIDG KLRAGVNYYILPV RGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           TLGNLQSE CPLNVVQEQ EVMNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRD 180
           WKLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           IGIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

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BLAST of Cla97C01G013760 vs. TrEMBL
Match: tr|A0A1S3ASR3|A0A1S3ASR3_CUCME (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.7e-102
Identity = 182/204 (89.22%), Postives = 191/204 (93.63%), Query Frame = 0


Query: 2   KNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTL 61
           KN GI   F+FILLAST +RFS ADASPEAV DIDG KLRAGVNYYILPV RGRGGGLTL
Sbjct: 3   KNFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTL 62

Query: 62  GNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWK 121
           GNLQSE CP+NVVQEQFE+MNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWK
Sbjct: 63  GNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122

Query: 122 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDIG 181
           LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRDIG
Sbjct: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182

Query: 182 IFFKNGKRALALSDTPFPVMFKKV 206
           IFFKNGKRALALSDTPFPVMFKKV
Sbjct: 183 IFFKNGKRALALSDTPFPVMFKKV 205

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BLAST of Cla97C01G013760 vs. TrEMBL
Match: tr|A0A2N9HQC2|A0A2N9HQC2_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS42080 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.6e-74
Identity = 127/180 (70.56%), Postives = 155/180 (86.11%), Query Frame = 0


Query: 25  ADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPLNVVQEQFEVMNGL 84
           AD++P+ V D+ G+KLR GV+YYILPVIRGRGGGLTL + +++TCPL+VVQEQ E+ NGL
Sbjct: 16  ADSAPDPVPDLAGEKLRTGVDYYILPVIRGRGGGLTLASTRNKTCPLDVVQEQHEISNGL 75

Query: 85  PTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPG 144
           P TF+ VNPKKGV+RVSTDLN++F A+TIC  STVWKLDKFDEST QWFVT GG  GNPG
Sbjct: 76  PLTFSSVNPKKGVVRVSTDLNIKFSAATICVQSTVWKLDKFDESTRQWFVTTGGVEGNPG 135

Query: 145 VETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDIGIFFKNGKRALALSDTPFPVMFKK 204
            ET  NW+KIEK+  D+KLVFCP+VCDFCKV+CRD+GI+ ++G+R+LALSD PF VMFKK
Sbjct: 136 RETTSNWYKIEKYDDDFKLVFCPTVCDFCKVLCRDVGIYIEDGRRSLALSDVPFKVMFKK 195

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BLAST of Cla97C01G013760 vs. TrEMBL
Match: tr|A0A1S3AST3|A0A1S3AST3_CUCME (miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 5.9e-74
Identity = 129/206 (62.62%), Postives = 166/206 (80.58%), Query Frame = 0


Query: 1   MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 60
           MKN   LL FLFI++AS+ VRF RADASP+AV D DG KLRAG  YYIL V     GGL+
Sbjct: 1   MKNFA-LLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLS 60

Query: 61  LGNLQS-ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           +G +   E CP+N++ E ++ ++GLP TF+PVNPKKGV+RVSTDLN++FEAST C  STV
Sbjct: 61  IGGIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRD 180
           WK+ KFD+   Q+FVT+GG++GNPG ET+ NWFK+EKHG++YK V+CP+VC +CKVMC+D
Sbjct: 121 WKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           +G+F+KNG+R  AL+D PFPVMFKKV
Sbjct: 181 VGLFYKNGRRIFALNDAPFPVMFKKV 205

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BLAST of Cla97C01G013760 vs. TrEMBL
Match: tr|A0A0A0LT81|A0A0A0LT81_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.3e-73
Identity = 127/206 (61.65%), Postives = 168/206 (81.55%), Query Frame = 0


Query: 1   MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 60
           M+N   LL FLFI++AS+ VRF RADASP+AV D DG KLRAG  YYIL V     GGL+
Sbjct: 1   MRNFA-LLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLS 60

Query: 61  LGNLQS-ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           +G +   E CP+N++ E ++ ++GLP TF+P+NPKKGV+RVSTDLN+QFEA+T C  STV
Sbjct: 61  IGGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRD 180
           WK+ KFDE   Q+FVT+GG +GNPG ET++NWFK+EK+G++YKLV+CP+VC +CKV+C+D
Sbjct: 121 WKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           +G+F+KNG+R +AL+D PFPVMFKKV
Sbjct: 181 VGLFYKNGRRVIALNDAPFPVMFKKV 205

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BLAST of Cla97C01G013760 vs. Swiss-Prot
Match: sp|Q9LMU2|KTI2_ARATH (Kunitz trypsin inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=KTI2 PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 6.5e-60
Identity = 109/198 (55.05%), Postives = 148/198 (74.75%), Query Frame = 0


Query: 8   LSFLFILLASTVV-RFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQS 67
           L ++F+LLA  +  R    +A+ E V+DI+G  L  GVNYYILPVIRGRGGGLT+ NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 68  ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 127
           ETCP +V+Q+QFEV  GLP  F+P + K   I VSTD+N++F      + +++W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANFD 123

Query: 128 ESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDIGIFFKN 187
           E+T QWF++  G  GNPG +TVDNWFKI+K  +DYK+ FCP+VC+FCKV+CRD+G+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 188 GKRALALSDTPFPVMFKK 205
           GKR LALSD P  VMFK+
Sbjct: 184 GKRRLALSDVPLKVMFKR 194

[Close]

BLAST of Cla97C01G013760 vs. Swiss-Prot
Match: sp|P13087|MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 214.2 bits (544), Expect = 1.4e-54
Identity = 116/216 (53.70%), Postives = 143/216 (66.20%), Query Frame = 0


Query: 1   MKNLGIL-LSFLFI---LLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRG 60
           MK L +L LSF F+   L A+     S AD++P  V DIDG+KLR G NYYI+PV+R  G
Sbjct: 1   MKELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHG 60

Query: 61  GGLTLGNLQSE---TCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTI 120
           GGLT+          CP  VVQ + EV +  P  F P NPK+ V+RVSTDLN+ F A   
Sbjct: 61  GGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 120

Query: 121 C--ATSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKH--GRDYKLVFCPSV 180
           C   +STVW+LDK+DESTGQ+FVTIGG +GNPG ET+ +WFKIE+      YKLVFCP+V
Sbjct: 121 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTV 180

Query: 181 CDFCKVMCRDIGIFF-KNGKRALALSDTPFPVMFKK 205
           C  CKV C D+GI+  + G+R LALSD PF   F K
Sbjct: 181 CGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]

BLAST of Cla97C01G013760 vs. Swiss-Prot
Match: sp|P32765|ASP_THECC (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 7.2e-43
Identity = 95/204 (46.57%), Postives = 125/204 (61.27%), Query Frame = 0


Query: 6   ILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGR-GGGLTLGNL 65
           +LL F F    S    F  A+A+   V D DGD+L+ GV YY+L  I G  GGGL LG  
Sbjct: 8   VLLLFAF---TSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRA 67

Query: 66  QSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFE--ASTICATSTVWKL 125
             ++CP  VVQ + ++ NG P  F+  + K  V+RVSTD+N++F      +C+TSTVW+L
Sbjct: 68  TGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRL 127

Query: 126 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHG-RDYKLVFCPSVCDFCKVMCRDIG 185
           D +D S G+W+VT  G +G PG  T+ +WFKIEK G   YK  FCPSVCD C  +C DIG
Sbjct: 128 DNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIG 187

Query: 186 IFF-KNGKRALALSDTPFPVMFKK 205
                +G+  LALSD  +  MFKK
Sbjct: 188 RHSDDDGQIRLALSDNEWAWMFKK 208

[Close]

BLAST of Cla97C01G013760 vs. Swiss-Prot
Match: sp|Q8RXD5|KTI1_ARATH (Kunitz trypsin inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=KTI1 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.3e-36
Identity = 88/196 (44.90%), Postives = 116/196 (59.18%), Query Frame = 0


Query: 12  FILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPL 71
           +++LA T V  S A     AV DIDG+ +    +YY+LPVIRGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFE-ASTICATSTVWKLDKFDESTG 131
           ++VQE  EV  G+P  F+    K   +  S +LN++ +  +TIC  ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKHGRD-YKLVFCPSVCDFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G D YK VFCP  CD     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of Cla97C01G013760 vs. Swiss-Prot
Match: sp|P80691|LSPI_CARPA (Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 6.8e-25
Identity = 71/183 (38.80%), Postives = 104/183 (56.83%), Query Frame = 0


Query: 28  SPEAVRDIDGDKLRAGVNYYILPVIRGR-GGGLTL-GNLQSETCPLNVVQEQFEVMNGLP 87
           +P+ + DIDG  +  GV+Y+++  I G  GGGLT+ G    + CPL+VVQ+ F+  NG P
Sbjct: 2   APKPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKKCPLSVVQDPFD--NGEP 61

Query: 88  TTFAPV-NPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPG 147
             F+ + N K  ++  S DLNV+F  +  C  +T WK+D+F    G W VT+GG +G  G
Sbjct: 62  IIFSAIKNVKDNIVFESVDLNVKFNITINCNETTAWKVDRFPGVIG-WTVTLGGEKGYHG 121

Query: 148 VETVDNWFKIEKHGR--DYKLVFCPSVCDFCKVMCRDIGIFF-KNGKRALALSDTPFPVM 205
            E+  + FKI+K G    YK  FCPS      + C ++ IFF K   R L L++     +
Sbjct: 122 FESTHSMFKIKKAGLPFSYKFHFCPSYPRTRLIPCNNVDIFFDKYRIRRLILTNDAKEFV 181

[Close]

BLAST of Cla97C01G013760 vs. TAIR10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 231.9 bits (590), Expect = 3.6e-61
Identity = 109/198 (55.05%), Postives = 148/198 (74.75%), Query Frame = 0


Query: 8   LSFLFILLASTVV-RFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQS 67
           L ++F+LLA  +  R    +A+ E V+DI+G  L  GVNYYILPVIRGRGGGLT+ NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 68  ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 127
           ETCP +V+Q+QFEV  GLP  F+P + K   I VSTD+N++F      + +++W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANFD 123

Query: 128 ESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDIGIFFKN 187
           E+T QWF++  G  GNPG +TVDNWFKI+K  +DYK+ FCP+VC+FCKV+CRD+G+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 188 GKRALALSDTPFPVMFKK 205
           GKR LALSD P  VMFK+
Sbjct: 184 GKRRLALSDVPLKVMFKR 194

[Close]

BLAST of Cla97C01G013760 vs. TAIR10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1)

HSP 1 Score: 154.5 bits (389), Expect = 7.3e-38
Identity = 88/196 (44.90%), Postives = 116/196 (59.18%), Query Frame = 0


Query: 12  FILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPL 71
           +++LA T V  S A     AV DIDG+ +    +YY+LPVIRGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFE-ASTICATSTVWKLDKFDESTG 131
           ++VQE  EV  G+P  F+    K   +  S +LN++ +  +TIC  ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKHGRD-YKLVFCPSVCDFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G D YK VFCP  CD     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of Cla97C01G013760 vs. TAIR10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 106.3 bits (264), Expect = 2.3e-23
Identity = 72/208 (34.62%), Postives = 115/208 (55.29%), Query Frame = 0


Query: 6   ILLSFLFILLASTV-VRFSRADASP-EAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGN 65
           + LSF+ + + S +    S ADA+P + V DI G  +++ V YYI+P   G GGGL   N
Sbjct: 4   LTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSN 63

Query: 66  LQSET----CPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEAST-ICATST 125
               T      L++VQ     ++GLP TF+P+N K   +++S  LN++F+++  +C  S 
Sbjct: 64  RNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSK 123

Query: 126 VWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCR 185
           VW++D       + FV+IGG +G       ++WF+I++ G  YKL++CP       V C 
Sbjct: 124 VWRID-HSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIVACI 183

Query: 186 DIGI-FFKNGKRALALS-DTPFPVMFKK 205
           ++ +    +G R L LS D  F V F+K
Sbjct: 184 NVSLEIDDHGVRRLVLSTDQSFVVKFQK 202

The following BLAST results are available for this feature:

BLAST of Cla97C01G013760 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137534.1	4.1e-103	89.81	PREDICTED: miraculin-like [Cucumis sativus] >KGN64217.1 Tumor-related protein [C...	[more]
XP_008437058.1	2.6e-102	89.22	PREDICTED: miraculin-like [Cucumis melo]	[more]
XP_022923703.1	8.0e-99	86.83	kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin i...	[more]
XP_023520252.1	1.8e-98	86.83	kunitz trypsin inhibitor 2 [Cucurbita pepo subsp. pepo]	[more]
XP_022137472.1	2.5e-92	80.00	kunitz trypsin inhibitor 2 [Momordica charantia]	[more]

BLAST of Cla97C01G013760 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LTW2\|A0A0A0LTW2_CUCSA	2.7e-103	89.81	Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=4 SV=1	[more]
tr\|A0A1S3ASR3\|A0A1S3ASR3_CUCME	1.7e-102	89.22	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=4 SV=1	[more]
tr\|A0A2N9HQC2\|A0A2N9HQC2_FAGSY	2.6e-74	70.56	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS42080 PE=4 SV=1	[more]
tr\|A0A1S3AST3\|A0A1S3AST3_CUCME	5.9e-74	62.62	miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=4 SV=1	[more]
tr\|A0A0A0LT81\|A0A0A0LT81_CUCSA	1.3e-73	61.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=4 SV=1	[more]

BLAST of Cla97C01G013760 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9LMU2\|KTI2_ARATH	6.5e-60	55.05	Kunitz trypsin inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=KTI2 PE=2 SV=1	[more]
sp\|P13087\|MIRA_SYNDU	1.4e-54	53.70	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
sp\|P32765\|ASP_THECC	7.2e-43	46.57	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
sp\|Q8RXD5\|KTI1_ARATH	1.3e-36	44.90	Kunitz trypsin inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=KTI1 PE=2 SV=1	[more]
sp\|P80691\|LSPI_CARPA	6.8e-25	38.80	Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1	[more]

BLAST of Cla97C01G013760 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G17860.1	3.6e-61	55.05	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	7.3e-38	44.90	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	2.3e-23	34.62	Kunitz family trypsin and protease inhibitor protein	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011065	Kunitz_inhibitor_STI-like_sf
IPR002160	Prot_inh_Kunz-lg

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0008233	peptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C01G013760.1	Cla97C01G013760.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 31..60 score: 44.51 coord: 174..203 score: 25.03 coord: 69..89 score: 34.45 coord: 151..170 score: 25.29
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 31..205 e-value: 1.2E-75 score: 267.3
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 32..204 e-value: 3.7E-61 score: 206.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 1..205
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 32..48
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 31..204 e-value: 2.95419E-58 score: 182.542
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 29..205 e-value: 2.4E-73 score: 247.7
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	SSF50386	STI-like	coord: 28..205

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla97C01G013760	Cla008374	Watermelon (97103) v1	wmwmbB244
Cla97C01G013760	Csa1G043200	Cucumber (Chinese Long) v2	cuwmbB010
Cla97C01G013760	MELO3C002177	Melon (DHL92) v3.5.1	mewmbB133
Cla97C01G013760	ClCG01G014760	Watermelon (Charleston Gray)	wcgwmbB089
Cla97C01G013760	CSPI01G07600	Wild cucumber (PI 183967)	cpiwmbB010
Cla97C01G013760	Cucsa.122330	Cucumber (Gy14) v1	cgywmbB185
Cla97C01G013760	CmaCh13G009570	Cucurbita maxima (Rimu)	cmawmbB207
Cla97C01G013760	Lsi02G002790	Bottle gourd (USVL1VR-Ls)	lsiwmbB158
Cla97C01G013760	CsGy1G007470	Cucumber (Gy14) v2	cgybwmbB010
Cla97C01G013760	MELO3C002177.2	Melon (DHL92) v3.6.1	medwmbB125
Cla97C01G013760	Carg04878	Silver-seed gourd	carwmbB0406
Cla97C01G013760	CsaV3_1G007570	Cucumber (Chinese Long) v3	cucwmbB009
Cla97C01G013760	Bhi08G001119	Wax gourd	wgowmbB457

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla97C01G013760	Silver-seed gourd	carwmbB0516
Cla97C01G013760	Cucurbita maxima (Rimu)	cmawmbB399
Cla97C01G013760	Cucurbita moschata (Rifu)	cmowmbB191
Cla97C01G013760	Cucurbita moschata (Rifu)	cmowmbB383