CSPI01G07600 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI01G07600
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionKunitz trypsin inhibitor
LocationChr1 : 4820888 .. 4821719 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAACCTTTCCCTATCGAATTAAGCAACCCAACAAAAGAAAGTCCACATTGTTAATAAATAAAATGAAGAATTTCGGAATATTATTCTATTTTCTTTTCATTCTCCTTGCCTCAACCCAGCTGATTCGCTTCTCCACAGCCGACGCTTCGCCGGAGGCCGTTCTCGACATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCCGTTTTCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGAAATGTCCACTCAACGTCGTTCAAGAACAACTCGAAGTAATGAACGGATTTCCAACAACATTTCATCCTGTAAACCCTAAAAAGGGAGTGGTCCGAGTTTCAACCGATTTGAATGTACAATTCGAGGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGATGAATCGACCGGACAATGGTTGGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAGACGGTGGATAACTGGTTCAAAATTGAGAAGCATGGTAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGTAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAGAAAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAATTGAAAGATTAAAGTGAATGAGTTTGTGAAATAGTTTGTGTTTAATTCATGTATAAGTGTTTTATCTTAATTAAATAAAAGATATGGTTATTGTTTGTTTATTATTAGAAGAATAAAATACTCTATTGGATTGTTTTTCTATAAACG

mRNA sequence

ATGAAGAATTTCGGAATATTATTCTATTTTCTTTTCATTCTCCTTGCCTCAACCCAGCTGATTCGCTTCTCCACAGCCGACGCTTCGCCGGAGGCCGTTCTCGACATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCCGTTTTCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGAAATGTCCACTCAACGTCGTTCAAGAACAACTCGAAGTAATGAACGGATTTCCAACAACATTTCATCCTGTAAACCCTAAAAAGGGAGTGGTCCGAGTTTCAACCGATTTGAATGTACAATTCGAGGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGATGAATCGACCGGACAATGGTTGGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAGACGGTGGATAACTGGTTCAAAATTGAGAAGCATGGTAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGTAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAGAAAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

Coding sequence (CDS)

ATGAAGAATTTCGGAATATTATTCTATTTTCTTTTCATTCTCCTTGCCTCAACCCAGCTGATTCGCTTCTCCACAGCCGACGCTTCGCCGGAGGCCGTTCTCGACATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCCGTTTTCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGAAATGTCCACTCAACGTCGTTCAAGAACAACTCGAAGTAATGAACGGATTTCCAACAACATTTCATCCTGTAAACCCTAAAAAGGGAGTGGTCCGAGTTTCAACCGATTTGAATGTACAATTCGAGGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGATGAATCGACCGGACAATGGTTGGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAGACGGTGGATAACTGGTTCAAAATTGAGAAGCATGGTAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGTAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAGAAAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA
Homology
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BLAST of CSPI01G07600 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 216.5 bits (550), Expect = 2.8e-55
Identity = 113/206 (54.85%), Postives = 136/206 (66.02%), Query Frame = 1 

Query: 8   FYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQS 67
           F+F+  LLA+      S AD++P  VLDIDG+KLR G NYYI+PV R  GGGLT+     
Sbjct: 11  FFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTP 70

Query: 68  EK---CPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTIC--VTSTVWK 127
                CP  VVQ + EV +  P  F P NPK+ VVRVSTDLN+ F A   C   +STVW+
Sbjct: 71  NGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWR 130

Query: 128 LDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKH--GKDYKLVFCPTVCNFCKVMCRD 187
           LDK+DESTGQ+ VTIGG +GNPG ET+ +WFKIE+      YKLVFCPTVC  CKV C D
Sbjct: 131 LDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGD 190

Query: 188 IGIFF-KNGERALALSDTPFPVMFKK 206
           +GI+  + G R LALSD PF   F K
Sbjct: 191 VGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]
BLAST of CSPI01G07600 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.3e-41
Identity = 88/188 (46.81%), Postives = 114/188 (60.64%), Query Frame = 1 

Query: 23  FSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGG-LTLGNLQSEKCPLNVVQEQLEV 82
           F  A+A+   VLD DG +L+ GV YY+L    G GGG L LG    + CP  VVQ + ++
Sbjct: 21  FGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRSDL 80

Query: 83  MNGFPTTFHPVNPKKGVVRVSTDLNVQFEA--STICVTSTVWKLDKFDESTGQWLVTIGG 142
            NG P  F   + K  VVRVSTD+N++F      +C TSTVW+LD +D S G+W VT  G
Sbjct: 81  DNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDG 140

Query: 143 SRGNPGVETVDNWFKIEKHG-KDYKLVFCPTVCNFCKVMCRDIGIFF-KNGERALALSDT 202
            +G PG  T+ +WFKIEK G   YK  FCP+VC+ C  +C DIG     +G+  LALSD 
Sbjct: 141 VKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIGRHSDDDGQIRLALSDN 200

Query: 203 PFPVMFKK 206
            +  MFKK
Sbjct: 201 EWAWMFKK 208

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BLAST of CSPI01G07600 vs. Swiss-Prot
Match: LSPI_CARPA (Latex serine proteinase inhibitor OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.0e-25
Identity = 71/183 (38.80%), Postives = 104/183 (56.83%), Query Frame = 1 

Query: 29  SPEAVLDIDGKKLRAGVNYYILPVFRGR-GGGLTL-GNLQSEKCPLNVVQEQLEVMNGFP 88
           +P+ ++DIDGK +  GV+Y+++    G  GGGLT+ G    +KCPL+VVQ+  +  NG P
Sbjct: 2   APKPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKKCPLSVVQDPFD--NGEP 61

Query: 89  TTFHPV-NPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNPG 148
             F  + N K  +V  S DLNV+F  +  C  +T WK+D+F    G W VT+GG +G  G
Sbjct: 62  IIFSAIKNVKDNIVFESVDLNVKFNITINCNETTAWKVDRFPGVIG-WTVTLGGEKGYHG 121

Query: 149 VETVDNWFKIEKHGK--DYKLVFCPTVCNFCKVMCRDIGIFF-KNGERALALSDTPFPVM 206
            E+  + FKI+K G    YK  FCP+      + C ++ IFF K   R L L++     +
Sbjct: 122 FESTHSMFKIKKAGLPFSYKFHFCPSYPRTRLIPCNNVDIFFDKYRIRRLILTNDAKEFV 181

[Close]
BLAST of CSPI01G07600 vs. Swiss-Prot
Match: DRTI_DELRE (Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.2e-23
Identity = 67/180 (37.22%), Postives = 94/180 (52.22%), Query Frame = 1 

Query: 31  EAVLDIDGKKLRAGVNYYILPVFRGR-GGGLTLGNLQSEKCPLNVVQEQLEVMNGFPTTF 90
           E V DI+G  +  G  YYI+    G  GGG+  G  +   CP++++QEQ ++  G P  F
Sbjct: 4   EKVYDIEGYPVFLGSEYYIVSAIIGAGGGGVRPGRTRGSMCPMSIIQEQSDLQMGLPVRF 63

Query: 91  HPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNPGVETV 150
                 +G +   T+L ++F     C  S+ W + K    +G+  V IGGS  +P  E V
Sbjct: 64  SSPEESQGKIYTDTELEIEFVEKPDCAESSKWVIVK---DSGEARVAIGGSEDHPQGELV 123

Query: 151 DNWFKIEKHGK-DYKLVFCPTVCNFCKVMCRDIGIFFKNGERALAL---SDTPFPVMFKK 206
             +FKIEK G   YKLVFCP         C DIGI ++ G R+L L    D+PF V+F K
Sbjct: 124 RGFFKIEKLGSLAYKLVFCP---KSSSGSCSDIGINYE-GRRSLVLKSSDDSPFRVVFVK 176

[Close]
BLAST of CSPI01G07600 vs. Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.8e-22
Identity = 66/184 (35.87%), Postives = 92/184 (50.00%), Query Frame = 1 

Query: 30  PEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQLEVMNGFPTTF 89
           P  V D DG +LRA  NYY+LP  R  GGGLT+      +CPL V QE     +G P   
Sbjct: 2   PPPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQEADGQRDGLPVRI 61

Query: 90  HPVN--PKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNPGVE 149
            P    P   ++R+STD+ + F A T CV ST W +D    S  + ++T  G   +P   
Sbjct: 62  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHIDSELVSGRRHVIT--GPVRDPSPS 121

Query: 150 TVDNWFKIEKHG----KDYKLVFCPTVCNFCKVMCRDIGIF--FKNGERALALSDTPFPV 206
             +N F+IEK+      +YKL+ C          C+D+G+F   K G   L  ++    V
Sbjct: 122 GRENAFRIEKYSGAEVHEYKLMACGD-------SCQDLGVFRDLKGGAWFLGATEPYHVV 176

[Close]
BLAST of CSPI01G07600 vs. TrEMBL
Match: A0A0A0LTW2_CUCSA (Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 1.8e-117
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 1 

Query: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60
           MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120
           TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180
           WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGERALALSDTPFPVMFKKV 207
           IGIFFKNGERALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

[Close]
BLAST of CSPI01G07600 vs. TrEMBL
Match: A0A0A0LT81_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 5.5e-74
Identity = 129/207 (62.32%), Postives = 168/207 (81.16%), Query Frame = 1 

Query: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60
           M+NF +L  FLFI++AS++ +RF  ADASP+AVLD DGKKLRAG  YYIL V+    GGL
Sbjct: 1   MRNFALLC-FLFIVIASSE-VRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGL 60

Query: 61  TLGNLQS-EKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTST 120
           ++G +   EKCP+N++ E  + ++G P TF P+NPKKGVVRVSTDLN+QFEA+T C  ST
Sbjct: 61  SIGGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGIST 120

Query: 121 VWKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCR 180
           VWK+ KFDE   Q+ VT+GG +GNPG ET++NWFK+EK+GK+YKLV+CPTVC +CKV+C+
Sbjct: 121 VWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCK 180

Query: 181 DIGIFFKNGERALALSDTPFPVMFKKV 207
           D+G+F+KNG R +AL+D PFPVMFKKV
Sbjct: 181 DVGLFYKNGRRVIALNDAPFPVMFKKV 205

[Close]
BLAST of CSPI01G07600 vs. TrEMBL
Match: A0A061EZK2_THECC (Kunitz family trypsin and protease inhibitor protein OS=Theobroma cacao GN=TCM_025233 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 3.5e-73
Identity = 128/180 (71.11%), Postives = 147/180 (81.67%), Query Frame = 1 

Query: 26  ADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQLEVMNGF 85
           A+A+P+ VLDI GKKLR G +YYILPVFRGRGGGLTL +  +E CPL+VVQEQLEV +G 
Sbjct: 15  ANAAPDPVLDISGKKLRTGTDYYILPVFRGRGGGLTLASTGNESCPLDVVQEQLEVSDGL 74

Query: 86  PTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNPG 145
           P TF PVN KKGVVRVSTD N++F A+TICV  T+WKLD FD+ST QW VT GG  GNPG
Sbjct: 75  PVTFSPVNIKKGVVRVSTDQNIKFSAATICVQPTLWKLDSFDDSTRQWFVTTGGVEGNPG 134

Query: 146 VETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGERALALSDTPFPVMFKK 205
            ET+DNWFKIEK+  DYKLVFCPTVC+FCKVMCRD+G+F   G R LALSD PF VMFK+
Sbjct: 135 RETIDNWFKIEKYEDDYKLVFCPTVCDFCKVMCRDVGVFIDGGVRRLALSDVPFKVMFKR 194

[Close]
BLAST of CSPI01G07600 vs. TrEMBL
Match: M5X024_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011496mg PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 4.6e-73
Identity = 134/198 (67.68%), Postives = 153/198 (77.27%), Query Frame = 1 

Query: 10  FLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSE- 69
           F F+L A +  +R   ADA+P  VLDI G KL+ GV+YYILPV RGRGGGLTL +  ++ 
Sbjct: 11  FCFLLFAFSAKLRSVAADAAPSPVLDITGNKLQTGVDYYILPVIRGRGGGLTLASTSNKT 70

Query: 70  KCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDE 129
            CPL+VVQEQ EV NG P  F PVN  KGVVRVSTDLN++F A+TICV STVWKL KFDE
Sbjct: 71  SCPLDVVQEQNEVSNGLPLKFSPVNVTKGVVRVSTDLNIKFSATTICVQSTVWKLGKFDE 130

Query: 130 STGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNG 189
            TGQW VT GG  GNPG +T  NWFKIEK G DYKLVFCPTVCNFCKV+C D+GIFF++G
Sbjct: 131 QTGQWFVTSGGVEGNPGRQTTSNWFKIEKFGDDYKLVFCPTVCNFCKVICGDVGIFFQDG 190

Query: 190 ERALALSDTPFPVMFKKV 207
           +R LALSD PF  MFKKV
Sbjct: 191 KRRLALSDVPFRAMFKKV 208

[Close]
BLAST of CSPI01G07600 vs. TrEMBL
Match: A5AXN3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027379 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 6.0e-73
Identity = 138/206 (66.99%), Postives = 162/206 (78.64%), Query Frame = 1 

Query: 1   MKNFGILFYFLFILLASTQLIRFSTA-DASPEAVLDIDGKKLRAGVNYYILPVFRGRGGG 60
           MK   +LF  L I LA      FS A +++P+ VLD +GKKLR+GV+YYILPVFRGRGGG
Sbjct: 1   MKTTSLLFSLLLIALAVKP---FSVAAESAPDPVLDTEGKKLRSGVDYYILPVFRGRGGG 60

Query: 61  LTLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTST 120
           LTL +  +E CPL+VVQEQ EV NG P TF PVNPKKGV+RVSTD N++F ASTICV ST
Sbjct: 61  LTLASTGNESCPLDVVQEQQEVSNGLPLTFTPVNPKKGVIRVSTDHNIKFSASTICVQST 120

Query: 121 VWKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCR 180
           +WKL+ +DES+GQW VT GG  GNPG ET+DNWFKIEK+  DYKLVFCPTVC+FCK +C 
Sbjct: 121 LWKLE-YDESSGQWFVTTGGVEGNPGRETLDNWFKIEKYEDDYKLVFCPTVCDFCKPVCG 180

Query: 181 DIGIFFKNGERALALSDTPFPVMFKK 206
           DIGI+ +NG R LALSD PF VMFKK
Sbjct: 181 DIGIYIQNGYRRLALSDVPFKVMFKK 202

[Close]
BLAST of CSPI01G07600 vs. TAIR10
Match: AT1G17860.1 (AT1G17860.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 229.2 bits (583), Expect = 2.4e-60
Identity = 107/196 (54.59%), Postives = 140/196 (71.43%), Query Frame = 1 

Query: 10  FLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEK 69
           ++F+LLA     R  T +A+ E V DI+GK L  GVNYYILPV RGRGGGLT+ NL++E 
Sbjct: 6   YIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTET 65

Query: 70  CPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDES 129
           CP +V+Q+Q EV  G P  F P + K   + VSTD+N++F  ++I      W+L  FDE+
Sbjct: 66  CPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPTSI------WELANFDET 125

Query: 130 TGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGE 189
           T QW ++  G  GNPG +TVDNWFKI+K  KDYK+ FCPTVCNFCKV+CRD+G+F ++G+
Sbjct: 126 TKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGK 185

Query: 190 RALALSDTPFPVMFKK 206
           R LALSD P  VMFK+
Sbjct: 186 RRLALSDVPLKVMFKR 194

[Close]
BLAST of CSPI01G07600 vs. TAIR10
Match: AT1G73260.1 (AT1G73260.1 kunitz trypsin inhibitor 1)

HSP 1 Score: 149.8 bits (377), Expect = 1.8e-36
Identity = 84/197 (42.64%), Postives = 115/197 (58.38%), Query Frame = 1 

Query: 12  FILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCP 71
           +++LA T ++    A  +  AV+DIDG  +    +YY+LPV RGRGGGLTL     + CP
Sbjct: 13  YLVLALTAVL----ASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCP 72

Query: 72  LNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFE-ASTICVTSTVWKLDKFDEST 131
            ++VQE  EV  G P  F     K   V  S +LN++ +  +TIC+ ST W++ +FD   
Sbjct: 73  YDIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHER 132

Query: 132 GQWLVTIGGSRGNPGVETVDNWFKIEKHGKD-YKLVFCPTVCNFCKVMCRDIGIFFKN-G 191
            Q+ V  G      G +++ ++FKIEK G+D YK VFCP  C+     C D+GIF    G
Sbjct: 133 KQYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELG 192

Query: 192 ERALALSDTPFPVMFKK 206
            R LALSD PF VMFKK
Sbjct: 193 VRRLALSDKPFLVMFKK 204

[Close]
BLAST of CSPI01G07600 vs. TAIR10
Match: AT1G73325.1 (AT1G73325.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 105.1 bits (261), Expect = 5.1e-23
Identity = 71/204 (34.80%), Postives = 111/204 (54.41%), Query Frame = 1 

Query: 10  FLFILLASTQLIRFSTADASP-EAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGN--LQ 69
           F+ + + S      S ADA+P + VLDI G  +++ V YYI+P   G GGGL   N  L 
Sbjct: 8   FITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLS 67

Query: 70  SEKCPLN--VVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEAST-ICVTSTVWKL 129
           ++   LN  +VQ     ++G P TF P+N K   V++S  LN++F+++  +C  S VW++
Sbjct: 68  TQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRI 127

Query: 130 DKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 189
           D       +  V+IGG +G       ++WF+I++ G  YKL++CP       V C ++ +
Sbjct: 128 D-HSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIVACINVSL 187

Query: 190 -FFKNGERALALS-DTPFPVMFKK 206
               +G R L LS D  F V F+K
Sbjct: 188 EIDDHGVRRLVLSTDQSFVVKFQK 202

[Close]
BLAST of CSPI01G07600 vs. NCBI nr
Match: gi|449439521|ref|XP_004137534.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 429.9 bits (1104), Expect = 2.6e-117
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 1 

Query: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60
           MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120
           TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180
           WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGERALALSDTPFPVMFKKV 207
           IGIFFKNGERALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

[Close]
BLAST of CSPI01G07600 vs. NCBI nr
Match: gi|659066983|ref|XP_008437058.1| (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 402.5 bits (1033), Expect = 4.4e-109
Identity = 193/205 (94.15%), Postives = 200/205 (97.56%), Query Frame = 1 

Query: 2   KNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 61
           KNFGI FYF+FILLAST+ +RFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 3   KNFGI-FYFIFILLASTE-LRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 62

Query: 62  LGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVW 121
           LGNLQSE CP+NVVQEQ E+MNGFPTTFHPVNPKKGVVRVSTDLNVQF+ASTICVTSTVW
Sbjct: 63  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 122

Query: 122 KLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 181
           KLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 123 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 182

Query: 182 GIFFKNGERALALSDTPFPVMFKKV 207
           GIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 183 GIFFKNGKRALALSDTPFPVMFKKV 205

[Close]
BLAST of CSPI01G07600 vs. NCBI nr
Match: gi|659066987|ref|XP_008437082.1| (PREDICTED: miraculin [Cucumis melo])

HSP 1 Score: 286.6 bits (732), Expect = 3.5e-74
Identity = 131/207 (63.29%), Postives = 166/207 (80.19%), Query Frame = 1 

Query: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60
           MKNF +L  FLFI++AS++ +RF  ADASP+AVLD DGKKLRAG  YYIL V+    GGL
Sbjct: 1   MKNFALLC-FLFIVIASSE-VRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGL 60

Query: 61  TLGNLQS-EKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTST 120
           ++G +   EKCP+N++ E  + ++G P TF PVNPKKGVVRVSTDLN++FEAST C  ST
Sbjct: 61  SIGGIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGIST 120

Query: 121 VWKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCR 180
           VWK+ KFD+   Q+ VT+GG++GNPG ET+ NWFK+EKHGK+YK V+CPTVC +CKVMC+
Sbjct: 121 VWKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCK 180

Query: 181 DIGIFFKNGERALALSDTPFPVMFKKV 207
           D+G+F+KNG R  AL+D PFPVMFKKV
Sbjct: 181 DVGLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]
BLAST of CSPI01G07600 vs. NCBI nr
Match: gi|449439731|ref|XP_004137639.1| (PREDICTED: miraculin [Cucumis sativus])

HSP 1 Score: 285.4 bits (729), Expect = 7.8e-74
Identity = 129/207 (62.32%), Postives = 168/207 (81.16%), Query Frame = 1 

Query: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60
           M+NF +L  FLFI++AS++ +RF  ADASP+AVLD DGKKLRAG  YYIL V+    GGL
Sbjct: 1   MRNFALLC-FLFIVIASSE-VRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGL 60

Query: 61  TLGNLQS-EKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTST 120
           ++G +   EKCP+N++ E  + ++G P TF P+NPKKGVVRVSTDLN+QFEA+T C  ST
Sbjct: 61  SIGGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGIST 120

Query: 121 VWKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCR 180
           VWK+ KFDE   Q+ VT+GG +GNPG ET++NWFK+EK+GK+YKLV+CPTVC +CKV+C+
Sbjct: 121 VWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCK 180

Query: 181 DIGIFFKNGERALALSDTPFPVMFKKV 207
           D+G+F+KNG R +AL+D PFPVMFKKV
Sbjct: 181 DVGLFYKNGRRVIALNDAPFPVMFKKV 205

[Close]
BLAST of CSPI01G07600 vs. NCBI nr
Match: gi|590638321|ref|XP_007029360.1| (Kunitz family trypsin and protease inhibitor protein [Theobroma cacao])

HSP 1 Score: 282.7 bits (722), Expect = 5.1e-73
Identity = 128/180 (71.11%), Postives = 147/180 (81.67%), Query Frame = 1 

Query: 26  ADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQLEVMNGF 85
           A+A+P+ VLDI GKKLR G +YYILPVFRGRGGGLTL +  +E CPL+VVQEQLEV +G 
Sbjct: 15  ANAAPDPVLDISGKKLRTGTDYYILPVFRGRGGGLTLASTGNESCPLDVVQEQLEVSDGL 74

Query: 86  PTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNPG 145
           P TF PVN KKGVVRVSTD N++F A+TICV  T+WKLD FD+ST QW VT GG  GNPG
Sbjct: 75  PVTFSPVNIKKGVVRVSTDQNIKFSAATICVQPTLWKLDSFDDSTRQWFVTTGGVEGNPG 134

Query: 146 VETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGERALALSDTPFPVMFKK 205
            ET+DNWFKIEK+  DYKLVFCPTVC+FCKVMCRD+G+F   G R LALSD PF VMFK+
Sbjct: 135 RETIDNWFKIEKYEDDYKLVFCPTVCDFCKVMCRDVGVFIDGGVRRLALSDVPFKVMFKR 194

The following BLAST results are available for this feature:
BLAST of CSPI01G07600 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MIRA_SYNDU2.8e-5554.85Miraculin OS=Synsepalum dulcificum PE=1 SV=3[more]
ASP_THECC2.3e-4146.8121 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1[more]
LSPI_CARPA1.0e-2538.80Latex serine proteinase inhibitor OS=Carica papaya PE=1 SV=1[more]
DRTI_DELRE2.2e-2337.22Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1[more]
IAAS_WHEAT1.8e-2235.87Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1[more]
BLAST of CSPI01G07600 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LTW2_CUCSA1.8e-117100.00Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1[more]
A0A0A0LT81_CUCSA5.5e-7462.32Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1[more]
A0A061EZK2_THECC3.5e-7371.11Kunitz family trypsin and protease inhibitor protein OS=Theobroma cacao GN=TCM_0... [more]
M5X024_PRUPE4.6e-7367.68Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011496mg PE=4 SV=1[more]
A5AXN3_VITVI6.0e-7366.99Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027379 PE=4 SV=1[more]
BLAST of CSPI01G07600 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G17860.12.4e-6054.59 Kunitz family trypsin and protease inhibitor protein[more]
AT1G73260.11.8e-3642.64 kunitz trypsin inhibitor 1[more]
AT1G73325.15.1e-2334.80 Kunitz family trypsin and protease inhibitor protein[more]
BLAST of CSPI01G07600 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449439521|ref|XP_004137534.1|2.6e-117100.00PREDICTED: miraculin-like [Cucumis sativus][more]
gi|659066983|ref|XP_008437058.1|4.4e-10994.15PREDICTED: miraculin-like [Cucumis melo][more]
gi|659066987|ref|XP_008437082.1|3.5e-7463.29PREDICTED: miraculin [Cucumis melo][more]
gi|449439731|ref|XP_004137639.1|7.8e-7462.32PREDICTED: miraculin [Cucumis sativus][more]
gi|590638321|ref|XP_007029360.1|5.1e-7371.11Kunitz family trypsin and protease inhibitor protein [Theobroma cacao][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002160Prot_inh_Kunz-lg
IPR011065Kunitz_inhibitor_STI-like_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004866endopeptidase inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004866 endopeptidase inhibitor activity
molecular_function GO:0008233 peptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G07600.1CSPI01G07600.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumePRINTSPR00291KUNITZINHBTRcoord: 70..90
score: 9.3E-19coord: 32..61
score: 9.3E-19coord: 175..204
score: 9.3E-19coord: 152..171
score: 9.3
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 33..205
score: 1.7
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 32..206
score: 7.3
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 33..49
scor
IPR011065Kunitz inhibitor ST1-likeunknownSSF50386STI-likecoord: 29..206
score: 7.85
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 29..206
score: 5.9
NoneNo IPR availablePANTHERPTHR33107FAMILY NOT NAMEDcoord: 1..206
score: 4.6