ClCG01G014760 (gene) Watermelon (Charleston Gray)

Overview
NameClCG01G014760
Typegene
OrganismCitrullus lanatus (Watermelon (Charleston Gray))
DescriptionKunitz trypsin inhibitor
LocationCG_Chr01 : 29085598 .. 29086215 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAATTTGGGAATATTACTTTCTTTTCTTTTCATTCTCCTTGCCTCTACCGTGGTCCGCTTCTCCAGAGCCGACGCTTCGCCGGAGGCCGTCCGCGACATCGACGGCGATAAGCTCCGAGCCGGCGTCAATTATTACATCCTCCCTGTTATCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGACATGTCCACTCAACGTGGTTCAAGAACAATTCGAAGTGATGAACGGCTTGCCTACAACATTTGCGCCTGTAAACCCTAAAAAGGGAGTGATTCGAGTTTCGACGGATTTGAACGTGCAATTCGAGGCAAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTCGGGATTACAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCAGTTATGTTCAAGAAAGTTTGA

mRNA sequence

ATGAAGAATTTGGGAATATTACTTTCTTTTCTTTTCATTCTCCTTGCCTCTACCGTGGTCCGCTTCTCCAGAGCCGACGCTTCGCCGGAGGCCGTCCGCGACATCGACGGCGATAAGCTCCGAGCCGGCGTCAATTATTACATCCTCCCTGTTATCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGACATGTCCACTCAACGTGGTTCAAGAACAATTCGAAGTGATGAACGGCTTGCCTACAACATTTGCGCCTGTAAACCCTAAAAAGGGAGTGATTCGAGTTTCGACGGATTTGAACGTGCAATTCGAGGCAAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTCGGGATTACAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCAGTTATGTTCAAGAAAGTTTGA

Coding sequence (CDS)

ATGAAGAATTTGGGAATATTACTTTCTTTTCTTTTCATTCTCCTTGCCTCTACCGTGGTCCGCTTCTCCAGAGCCGACGCTTCGCCGGAGGCCGTCCGCGACATCGACGGCGATAAGCTCCGAGCCGGCGTCAATTATTACATCCTCCCTGTTATCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGACATGTCCACTCAACGTGGTTCAAGAACAATTCGAAGTGATGAACGGCTTGCCTACAACATTTGCGCCTGTAAACCCTAAAAAGGGAGTGATTCGAGTTTCGACGGATTTGAACGTGCAATTCGAGGCAAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTCGGGATTACAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCAGTTATGTTCAAGAAAGTTTGA

Protein sequence

MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDIGIFFKNGKRALALSDTPFPVMFKKV
Homology
[Close]
BLAST of ClCG01G014760 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 211.5 bits (537), Expect = 8.9e-54
Identity = 116/216 (53.70%), Postives = 142/216 (65.74%), Query Frame = 1 

Query: 1   MKNLGIL-LSFLFI---LLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRG 60
           MK L +L LSF F+   L A+     S AD++P  V DIDG+KLR G NYYI+PV+R  G
Sbjct: 1   MKELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHG 60

Query: 61  GGLTLGNLQSE---TCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTI 120
           GGLT+          CP  VVQ + EV +  P  F P NPK+ V+RVSTDLN+ F A   
Sbjct: 61  GGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 120

Query: 121 C--ATSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKH--GRDYKLVFCPSV 180
           C   +STVW+LDK+DESTGQ+FVTIGG +GNPG ET+ +WFKIE+      YKLVFCP+V
Sbjct: 121 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTV 180

Query: 181 CDFCKVMCRDIGIFF-KNGKRALALSDTPFPVMFKK 205
           C  CKV C D+GI+  + G+R LALSD PF   F K
Sbjct: 181 CGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]
BLAST of ClCG01G014760 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 7.8e-42
Identity = 95/204 (46.57%), Postives = 123/204 (60.29%), Query Frame = 1 

Query: 6   ILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGG-LTLGNL 65
           +LL F F    S    F  A+A+   V D DGD+L+ GV YY+L  I G GGG L LG  
Sbjct: 8   VLLLFAF---TSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRA 67

Query: 66  QSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEA--STICATSTVWKL 125
             ++CP  VVQ + ++ NG P  F+  + K  V+RVSTD+N++F      +C+TSTVW+L
Sbjct: 68  TGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRL 127

Query: 126 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHG-RDYKLVFCPSVCDFCKVMCRDIG 185
           D +D S G+W+VT  G +G PG  T+ +WFKIEK G   YK  FCPSVCD C  +C DIG
Sbjct: 128 DNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIG 187

Query: 186 IFF-KNGKRALALSDTPFPVMFKK 205
                +G+  LALSD  +  MFKK
Sbjct: 188 RHSDDDGQIRLALSDNEWAWMFKK 208

[Close]
BLAST of ClCG01G014760 vs. Swiss-Prot
Match: PIP21_PEA (Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 9.6e-24
Identity = 74/198 (37.37%), Postives = 99/198 (50.00%), Query Frame = 1 

Query: 10  FLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRG-RGGGLTLGNLQSET 69
           FLF+ + +  + FS  D   E V D +G+ +  GV Y+ILP IRG  GGG+ LG     T
Sbjct: 12  FLFVFITNLSLAFSNEDV--EQVLDFNGNPIFPGVQYFILPAIRGPAGGGVRLGRTGDLT 71

Query: 70  CPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDES 129
           CP+ V+Q++ EV NGLP  F       G+I   T + +++     CA S+ W L   D  
Sbjct: 72  CPVTVLQDRQEVKNGLPVKFVIPEISPGIIFTGTPIEIEYTKKPNCAKSSKW-LVFVDNV 131

Query: 130 TGQWFVTIGGSRGNPGVETVDNWFKIEKH--GRDYKLVFCPSVCDFCKVMCRDIGIF--F 189
             +  V IGG    PGV+T+   FKIEKH  G  YKL FC          C D+G F   
Sbjct: 132 IQKACVGIGGPENYPGVQTLSGLFKIEKHESGFGYKLGFCVK----GSPTCLDVGRFDND 191

Query: 190 KNGKRALALSDTPFPVMF 203
           ++G+R        F V+F
Sbjct: 192 EDGRRLNLTEHESFQVVF 202

[Close]
BLAST of ClCG01G014760 vs. Swiss-Prot
Match: LSPI_CARPA (Latex serine proteinase inhibitor OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.1e-23
Identity = 71/183 (38.80%), Postives = 102/183 (55.74%), Query Frame = 1 

Query: 28  SPEAVRDIDGDKLRAGVNYYILPVIRGRGGG-LTL-GNLQSETCPLNVVQEQFEVMNGLP 87
           +P+ + DIDG  +  GV+Y+++  I G GGG LT+ G    + CPL+VVQ+ F+  NG P
Sbjct: 2   APKPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKKCPLSVVQDPFD--NGEP 61

Query: 88  TTFAPV-NPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPG 147
             F+ + N K  ++  S DLNV+F  +  C  +T WK+D+F    G W VT+GG +G  G
Sbjct: 62  IIFSAIKNVKDNIVFESVDLNVKFNITINCNETTAWKVDRFPGVIG-WTVTLGGEKGYHG 121

Query: 148 VETVDNWFKIEKHGR--DYKLVFCPSVCDFCKVMCRDIGIFF-KNGKRALALSDTPFPVM 205
            E+  + FKI+K G    YK  FCPS      + C ++ IFF K   R L L++     +
Sbjct: 122 FESTHSMFKIKKAGLPFSYKFHFCPSYPRTRLIPCNNVDIFFDKYRIRRLILTNDAKEFV 181

[Close]
BLAST of ClCG01G014760 vs. Swiss-Prot
Match: DRTI_DELRE (Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.8e-23
Identity = 71/180 (39.44%), Postives = 97/180 (53.89%), Query Frame = 1 

Query: 30  EAVRDIDGDKLRAGVNYYILPVIRGR-GGGLTLGNLQSETCPLNVVQEQFEVMNGLPTTF 89
           E V DI+G  +  G  YYI+  I G  GGG+  G  +   CP++++QEQ ++  GLP  F
Sbjct: 4   EKVYDIEGYPVFLGSEYYIVSAIIGAGGGGVRPGRTRGSMCPMSIIQEQSDLQMGLPVRF 63

Query: 90  APVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPGVETV 149
           +     +G I   T+L ++F     CA S+ W + K    +G+  V IGGS  +P  E V
Sbjct: 64  SSPEESQGKIYTDTELEIEFVEKPDCAESSKWVIVK---DSGEARVAIGGSEDHPQGELV 123

Query: 150 DNWFKIEKHGR-DYKLVFCPSVCDFCKVMCRDIGIFFKNGKRALAL---SDTPFPVMFKK 205
             +FKIEK G   YKLVFCP         C DIGI ++ G+R+L L    D+PF V+F K
Sbjct: 124 RGFFKIEKLGSLAYKLVFCPK---SSSGSCSDIGINYE-GRRSLVLKSSDDSPFRVVFVK 176

[Close]
BLAST of ClCG01G014760 vs. TrEMBL
Match: A0A0A0LTW2_CUCSA (Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 9.5e-103
Identity = 185/206 (89.81%), Postives = 192/206 (93.20%), Query Frame = 1 

Query: 1   MKNLGILLSFLFILLAST-VVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGL 60
           MKN GIL  FLFILLAST ++RFS ADASPEAV DIDG KLRAGVNYYILPV RGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           TLGNLQSE CPLNVVQEQ EVMNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRD 180
           WKLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           IGIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

[Close]
BLAST of ClCG01G014760 vs. TrEMBL
Match: A0A0A0LT81_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 6.0e-73
Identity = 127/206 (61.65%), Postives = 168/206 (81.55%), Query Frame = 1 

Query: 1   MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 60
           M+N   LL FLFI++AS+ VRF RADASP+AV D DG KLRAG  YYIL V     GGL+
Sbjct: 1   MRNFA-LLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLS 60

Query: 61  LGNLQS-ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           +G +   E CP+N++ E ++ ++GLP TF+P+NPKKGV+RVSTDLN+QFEA+T C  STV
Sbjct: 61  IGGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRD 180
           WK+ KFDE   Q+FVT+GG +GNPG ET++NWFK+EK+G++YKLV+CP+VC +CKV+C+D
Sbjct: 121 WKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           +G+F+KNG+R +AL+D PFPVMFKKV
Sbjct: 181 VGLFYKNGRRVIALNDAPFPVMFKKV 205

[Close]
BLAST of ClCG01G014760 vs. TrEMBL
Match: M5X024_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011496mg PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 1.9e-71
Identity = 136/200 (68.00%), Postives = 158/200 (79.00%), Query Frame = 1 

Query: 8   LSFLFILLA-STVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQS 67
           L F F+L A S  +R   ADA+P  V DI G+KL+ GV+YYILPVIRGRGGGLTL +  +
Sbjct: 9   LVFCFLLFAFSAKLRSVAADAAPSPVLDITGNKLQTGVDYYILPVIRGRGGGLTLASTSN 68

Query: 68  ET-CPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKF 127
           +T CPL+VVQEQ EV NGLP  F+PVN  KGV+RVSTDLN++F A+TIC  STVWKL KF
Sbjct: 69  KTSCPLDVVQEQNEVSNGLPLKFSPVNVTKGVVRVSTDLNIKFSATTICVQSTVWKLGKF 128

Query: 128 DESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDIGIFFK 187
           DE TGQWFVT GG  GNPG +T  NWFKIEK G DYKLVFCP+VC+FCKV+C D+GIFF+
Sbjct: 129 DEQTGQWFVTSGGVEGNPGRQTTSNWFKIEKFGDDYKLVFCPTVCNFCKVICGDVGIFFQ 188

Query: 188 NGKRALALSDTPFPVMFKKV 206
           +GKR LALSD PF  MFKKV
Sbjct: 189 DGKRRLALSDVPFRAMFKKV 208

[Close]
BLAST of ClCG01G014760 vs. TrEMBL
Match: A5AXN3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027379 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 2.5e-71
Identity = 138/205 (67.32%), Postives = 162/205 (79.02%), Query Frame = 1 

Query: 1   MKNLGILLSFLFILLASTVVRFS-RADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGL 60
           MK   +L S L I LA  V  FS  A+++P+ V D +G KLR+GV+YYILPV RGRGGGL
Sbjct: 1   MKTTSLLFSLLLIALA--VKPFSVAAESAPDPVLDTEGKKLRSGVDYYILPVFRGRGGGL 60

Query: 61  TLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           TL +  +E+CPL+VVQEQ EV NGLP TF PVNPKKGVIRVSTD N++F ASTIC  ST+
Sbjct: 61  TLASTGNESCPLDVVQEQQEVSNGLPLTFTPVNPKKGVIRVSTDHNIKFSASTICVQSTL 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRD 180
           WKL+ +DES+GQWFVT GG  GNPG ET+DNWFKIEK+  DYKLVFCP+VCDFCK +C D
Sbjct: 121 WKLE-YDESSGQWFVTTGGVEGNPGRETLDNWFKIEKYEDDYKLVFCPTVCDFCKPVCGD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKK 205
           IGI+ +NG R LALSD PF VMFKK
Sbjct: 181 IGIYIQNGYRRLALSDVPFKVMFKK 202

[Close]
BLAST of ClCG01G014760 vs. TrEMBL
Match: A5BCQ0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025776 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.1e-69
Identity = 135/204 (66.18%), Postives = 158/204 (77.45%), Query Frame = 1 

Query: 1   MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 60
           MK    L S L I LA      + A++SP+ V D +G +LR+GV+YYILPVIRGRGGGLT
Sbjct: 1   MKTTSFLFSLLLIALAVKPFPVA-AESSPDPVLDTEGKQLRSGVDYYILPVIRGRGGGLT 60

Query: 61  LGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 120
           L +  +E CPL+VVQEQ EV NGLP TF PVNPKKGVIRVSTD N++F ASTIC  ST+W
Sbjct: 61  LASTGNENCPLDVVQEQHEVSNGLPLTFTPVNPKKGVIRVSTDHNIKFSASTICVQSTLW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDI 180
           KL+ +DES+GQ FVT GG  GNPG ET+DNWFKIEK+  DYKLVFCP+VCDFCK +C DI
Sbjct: 121 KLE-YDESSGQRFVTTGGVEGNPGXETLDNWFKIEKYEDDYKLVFCPTVCDFCKPVCGDI 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKK 205
           GI+ +NG R LALSD PF VMFKK
Sbjct: 181 GIYIQNGYRRLALSDVPFKVMFKK 202

[Close]
BLAST of ClCG01G014760 vs. TAIR10
Match: AT1G17860.1 (AT1G17860.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 228.4 bits (581), Expect = 4.0e-60
Identity = 110/198 (55.56%), Postives = 145/198 (73.23%), Query Frame = 1 

Query: 8   LSFLFILLASTVV-RFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQS 67
           L ++F+LLA  +  R    +A+ E V+DI+G  L  GVNYYILPVIRGRGGGLT+ NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 68  ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 127
           ETCP +V+Q+QFEV  GLP  F+P + K   I VSTD+N++F  ++I      W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPTSI------WELANFD 123

Query: 128 ESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDIGIFFKN 187
           E+T QWF++  G  GNPG +TVDNWFKI+K  +DYK+ FCP+VC+FCKV+CRD+G+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 188 GKRALALSDTPFPVMFKK 205
           GKR LALSD P  VMFK+
Sbjct: 184 GKRRLALSDVPLKVMFKR 194

[Close]
BLAST of ClCG01G014760 vs. TAIR10
Match: AT1G73260.1 (AT1G73260.1 kunitz trypsin inhibitor 1)

HSP 1 Score: 150.6 bits (379), Expect = 1.1e-36
Identity = 88/196 (44.90%), Postives = 114/196 (58.16%), Query Frame = 1 

Query: 12  FILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPL 71
           +++LA T V  S A     AV DIDG+ +    +YY+LPVIRGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFE-ASTICATSTVWKLDKFDESTG 131
           ++VQE  EV  G+P  F+    K   +  S +LN++ +  +TIC  ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKHGRD-YKLVFCPSVCDFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G D YK VFCP  CD     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]
BLAST of ClCG01G014760 vs. TAIR10
Match: AT1G73325.1 (AT1G73325.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 104.4 bits (259), Expect = 8.7e-23
Identity = 72/208 (34.62%), Postives = 113/208 (54.33%), Query Frame = 1 

Query: 6   ILLSFLFILLASTV-VRFSRADASP-EAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGN 65
           + LSF+ + + S +    S ADA+P + V DI G  +++ V YYI+P   G GGGL   N
Sbjct: 4   LTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSN 63

Query: 66  LQSET----CPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEAST-ICATST 125
               T      L++VQ     ++GLP TF+P+N K   +++S  LN++F+++  +C  S 
Sbjct: 64  RNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSK 123

Query: 126 VWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCR 185
           VW++D       + FV+IGG +G       ++WF+I++ G  YKL++CP       V C 
Sbjct: 124 VWRID-HSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIVACI 183

Query: 186 DIGI-FFKNGKRALALS-DTPFPVMFKK 205
           ++ +    +G R L LS D  F V F+K
Sbjct: 184 NVSLEIDDHGVRRLVLSTDQSFVVKFQK 202

[Close]
BLAST of ClCG01G014760 vs. NCBI nr
Match: gi|449439521|ref|XP_004137534.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 380.9 bits (977), Expect = 1.4e-102
Identity = 185/206 (89.81%), Postives = 192/206 (93.20%), Query Frame = 1 

Query: 1   MKNLGILLSFLFILLAST-VVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGL 60
           MKN GIL  FLFILLAST ++RFS ADASPEAV DIDG KLRAGVNYYILPV RGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           TLGNLQSE CPLNVVQEQ EVMNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRD 180
           WKLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           IGIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

[Close]
BLAST of ClCG01G014760 vs. NCBI nr
Match: gi|659066983|ref|XP_008437058.1| (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 377.9 bits (969), Expect = 1.2e-101
Identity = 182/204 (89.22%), Postives = 191/204 (93.63%), Query Frame = 1 

Query: 2   KNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTL 61
           KN GI   F+FILLAST +RFS ADASPEAV DIDG KLRAGVNYYILPV RGRGGGLTL
Sbjct: 3   KNFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTL 62

Query: 62  GNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWK 121
           GNLQSE CP+NVVQEQFE+MNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWK
Sbjct: 63  GNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122

Query: 122 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDIG 181
           LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRDIG
Sbjct: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182

Query: 182 IFFKNGKRALALSDTPFPVMFKKV 206
           IFFKNGKRALALSDTPFPVMFKKV
Sbjct: 183 IFFKNGKRALALSDTPFPVMFKKV 205

[Close]
BLAST of ClCG01G014760 vs. NCBI nr
Match: gi|659066987|ref|XP_008437082.1| (PREDICTED: miraculin [Cucumis melo])

HSP 1 Score: 283.1 bits (723), Expect = 3.9e-73
Identity = 129/206 (62.62%), Postives = 166/206 (80.58%), Query Frame = 1 

Query: 1   MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 60
           MKN   LL FLFI++AS+ VRF RADASP+AV D DG KLRAG  YYIL V     GGL+
Sbjct: 1   MKNFA-LLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLS 60

Query: 61  LGNLQS-ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           +G +   E CP+N++ E ++ ++GLP TF+PVNPKKGV+RVSTDLN++FEAST C  STV
Sbjct: 61  IGGIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRD 180
           WK+ KFD+   Q+FVT+GG++GNPG ET+ NWFK+EKHG++YK V+CP+VC +CKVMC+D
Sbjct: 121 WKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           +G+F+KNG+R  AL+D PFPVMFKKV
Sbjct: 181 VGLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]
BLAST of ClCG01G014760 vs. NCBI nr
Match: gi|449439731|ref|XP_004137639.1| (PREDICTED: miraculin [Cucumis sativus])

HSP 1 Score: 282.0 bits (720), Expect = 8.6e-73
Identity = 127/206 (61.65%), Postives = 168/206 (81.55%), Query Frame = 1 

Query: 1   MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 60
           M+N   LL FLFI++AS+ VRF RADASP+AV D DG KLRAG  YYIL V     GGL+
Sbjct: 1   MRNFA-LLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLS 60

Query: 61  LGNLQS-ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           +G +   E CP+N++ E ++ ++GLP TF+P+NPKKGV+RVSTDLN+QFEA+T C  STV
Sbjct: 61  IGGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRD 180
           WK+ KFDE   Q+FVT+GG +GNPG ET++NWFK+EK+G++YKLV+CP+VC +CKV+C+D
Sbjct: 121 WKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           +G+F+KNG+R +AL+D PFPVMFKKV
Sbjct: 181 VGLFYKNGRRVIALNDAPFPVMFKKV 205

[Close]
BLAST of ClCG01G014760 vs. NCBI nr
Match: gi|595860796|ref|XP_007211211.1| (hypothetical protein PRUPE_ppa011496mg [Prunus persica])

HSP 1 Score: 276.9 bits (707), Expect = 2.8e-71
Identity = 136/200 (68.00%), Postives = 158/200 (79.00%), Query Frame = 1 

Query: 8   LSFLFILLA-STVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQS 67
           L F F+L A S  +R   ADA+P  V DI G+KL+ GV+YYILPVIRGRGGGLTL +  +
Sbjct: 9   LVFCFLLFAFSAKLRSVAADAAPSPVLDITGNKLQTGVDYYILPVIRGRGGGLTLASTSN 68

Query: 68  ET-CPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKF 127
           +T CPL+VVQEQ EV NGLP  F+PVN  KGV+RVSTDLN++F A+TIC  STVWKL KF
Sbjct: 69  KTSCPLDVVQEQNEVSNGLPLKFSPVNVTKGVVRVSTDLNIKFSATTICVQSTVWKLGKF 128

Query: 128 DESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGRDYKLVFCPSVCDFCKVMCRDIGIFFK 187
           DE TGQWFVT GG  GNPG +T  NWFKIEK G DYKLVFCP+VC+FCKV+C D+GIFF+
Sbjct: 129 DEQTGQWFVTSGGVEGNPGRQTTSNWFKIEKFGDDYKLVFCPTVCNFCKVICGDVGIFFQ 188

Query: 188 NGKRALALSDTPFPVMFKKV 206
           +GKR LALSD PF  MFKKV
Sbjct: 189 DGKRRLALSDVPFRAMFKKV 208

The following BLAST results are available for this feature:
BLAST of ClCG01G014760 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MIRA_SYNDU8.9e-5453.70Miraculin OS=Synsepalum dulcificum PE=1 SV=3[more]
ASP_THECC7.8e-4246.5721 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1[more]
PIP21_PEA9.6e-2437.37Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV... [more]
LSPI_CARPA2.1e-2338.80Latex serine proteinase inhibitor OS=Carica papaya PE=1 SV=1[more]
DRTI_DELRE2.8e-2339.44Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1[more]
BLAST of ClCG01G014760 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LTW2_CUCSA9.5e-10389.81Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1[more]
A0A0A0LT81_CUCSA6.0e-7361.65Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1[more]
M5X024_PRUPE1.9e-7168.00Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011496mg PE=4 SV=1[more]
A5AXN3_VITVI2.5e-7167.32Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027379 PE=4 SV=1[more]
A5BCQ0_VITVI1.1e-6966.18Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025776 PE=4 SV=1[more]
BLAST of ClCG01G014760 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G17860.14.0e-6055.56 Kunitz family trypsin and protease inhibitor protein[more]
AT1G73260.11.1e-3644.90 kunitz trypsin inhibitor 1[more]
AT1G73325.18.7e-2334.62 Kunitz family trypsin and protease inhibitor protein[more]
BLAST of ClCG01G014760 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449439521|ref|XP_004137534.1|1.4e-10289.81PREDICTED: miraculin-like [Cucumis sativus][more]
gi|659066983|ref|XP_008437058.1|1.2e-10189.22PREDICTED: miraculin-like [Cucumis melo][more]
gi|659066987|ref|XP_008437082.1|3.9e-7362.62PREDICTED: miraculin [Cucumis melo][more]
gi|449439731|ref|XP_004137639.1|8.6e-7361.65PREDICTED: miraculin [Cucumis sativus][more]
gi|595860796|ref|XP_007211211.1|2.8e-7168.00hypothetical protein PRUPE_ppa011496mg [Prunus persica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002160Prot_inh_Kunz-lg
IPR011065Kunitz_inhibitor_STI-like_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004866endopeptidase inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004866 endopeptidase inhibitor activity
molecular_function GO:0008233 peptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG01G014760.1ClCG01G014760.1mRNA


InterPro
Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumePRINTSPR00291KUNITZINHBTRcoord: 31..60
score: 2.0E-18coord: 174..203
score: 2.0E-18coord: 69..89
score: 2.0E-18coord: 151..170
score: 2.0
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 32..204
score: 3.6
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 31..205
score: 1.2
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 32..48
scor
IPR011065Kunitz inhibitor ST1-likeunknownSSF50386STI-likecoord: 28..205
score: 3.92
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 28..205
score: 2.3
NoneNo IPR availablePANTHERPTHR33107FAMILY NOT NAMEDcoord: 1..205
score: 3.4