Cucsa.398180 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.398180
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	ribonuclease 2
Location	scaffold04158 : 740088 .. 742473 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAAAAAAGAATGATAAAAGCGGGGAGTAGGAGGGTGGTGTTTGTAGTAGTTGTTGTTATTGGTATTGTTGTTGTTGTTGTTGACGGTGGGGGCTTCACCGTGGGGAAGGAACAGAGGGAATTTGATTACTTTATATTGGCTCTTCAATGGCCTTCCACTTCATGCACAAACGTAACCGTATGTTGCACCACCAACGCTTGCTGCCGCCGGTCAGTTCTTTTTAATTTCCTTCTCTCCTTCTTCGGTTTTCAAATGTTACACTCTCATTTCTGAACATCAAAAACATCATCTGATCTCTTTTATTAGTATTATTATTTTTGTGGTTCTTCATGTGATAGCCTGACCAAAATATTTCAAGCTGTACCATACTACTTCTTCTATTGTTTTTTTCATCATCTTTTTCTTATTCGAACAACTCTTTTAACACTTGTCAACTTCATATTATTAACATTGCTCTTTCTTTTGTTGCATGCATGATATGAGTTTTACTTTCTTGTTACATTCAGAAGTGCAATAATAATAATTAATGTTCTAATTACGATTTTTCTATTGTCTCTCTAGAGCTGTCTCTCCCACTGAATTCACAATACGTAAGTGTGAAGAGAAAAAACTCTTCCTTTAATTCACTTTGATTTTGCTTTGCATTTGGATGTTCATAAGAATTTTATTATCATTTTCATGATTTTTCTAAAAAACAAAGAAAAATTGCGCAGATGGGCTGTGGCCTAAGTACGAAGGAAAAGGATGGCCTTCATGTTGTACTAATGAAACCTTCGATGAAACTCAGGTCTCTCTCTCTCTCTCTCAAATTTAGAGGTTAGGAAGAATTTAAACTAGAGCAATATTTAGATACATGAATTCTTTGTGCATTCTAATCCAATCTCACACACAAAAAAATGTTATTAAAAAAAACGACACATACTATAAGATTGGACAATTGAATGAAATATAAAAGGATACCGAGTACTTTTCTCTGAAATATATATTCTATATTAGTCCTTTTGGTTTCTTTTAAAAAAAAATATCCTCCCTCTTAGTCTTACCTAACATACACAAAATGGACTAGTTAGATGAATCCATAATTTTTAAGATATTCATAAAACAAATAAATATACATTTTAACCTTTTGAAAAAAGTACTTATTTATATAGGTTAAAAAAATAGACATGTTTGGTCTGTGAGATAAGAAGGTTCAATTTAAGGAACATATAAAAAAGGGTACACTATTGTTATTGATATCATTAGGGTAATGCTCGCTATATATTTATCCATATGATCTAAAATATAATATACTCATCTCTTCCATATAACCAATAATATATTAACTCTAAACTTTCACTAATACTTGTAGATTCGTTCCCTGTTTGAAGATGTTGACAAGTATTGGCCAACCTATCGGTGTGGTCTTGTAAGCAGTTGTGATAATAGAAAAGGATCATTTTGGGCTCATCAGGTTCATACTTTTCTACCCAAAAATCTACTTTTAGTCCCAAATCTGACAATAATAAAAAAGCTAGCATTCAAATATTTAATGGTAAAAATTGAAGTCACAAACTAATAATACAATTGTTATAATATAATTTCTACCATTATGTAAGTCTCAGAGTTCAACTCTAACAGTTATACAGGTTTAAGTTTGAGGTATCCTGCCCACTACAATCATACTGAAGAGTAAATTTTACAATAGTTTACACAAAAACATTGTCTGCAAGCAGCTACAGTGATAGAAATGAATGATAATTAATTAAGGACTGAAAAAAGAGATTTTCTCGCAAATGTAGTGAGTTAAAATGTCCCTTTGAAAACTCAGCAACCCTAATCCTATATTGAAATTAAAAATGTATCTTTCTTCATTCTCTATGTCTATTTTTTCTTGCTTTGATCTTATTATAAATAAATTAGAGGCTCAATCCTAAAATTTCTCTCCTTTTCCTTTTTCATGATGGGCCTTTCACATGTTTCCATTGCTGCTCAGTATGGTATGTGTTTTTCTGTCCTTTTTTTCACCTCTTCCATCTGATCTAATGTTTTTCCTTTCTTTCTTCCATATTCTTCACCCATTCTTAAAGACATTTTTTTAAAGTTAAATATTTACTTATTGAGCAATGAAAGAATAAGTATTTTGAAAATATTTAAAAACACATTTTGTTAAGAACAAACAAACAGTACATACCATTATACGTGTAACTAATTTTTAGTTCCCTCTGCAACTTTTCTTTTTTTGTAGAGAAGCATGGAACCTGTGGATCGCCTGTAATCCTACAAGAATATGATTACTTCTTGACAACTCTCACCCTTTTCTACAAGTATAATGTCACTGTAAGCAACCTTTTACTAACTACACTTTATCCTCAATTTGTCTCATTAAGATTGTATCTTTCTTAG

mRNA sequence

GCAAAAAAGAATGATAAAAGCGGGGAGTAGGAGGGTGGTGTttgtagtagttgttgttattggtattgttgttgttgttgttgACGGTGGGGGCTTCACCGTGGGGAAGGAACAGAGGGAATTTGATTACTTTATATTGGCTCTTCAATGGCCTTCCACTTCATGCACAAACGTAACCGTATGTTGCACCACCAACGCTTGCTGCCGCCGAGCTGTCTCTCCCACTGAATTCACAATACATGGGCTGTGGCCTAAGTACGAAGGAAAAGGATGGCCTTCATGTTGTACTAATGAAACCTTCGATGAAACTCAGATTCGTTCCCTGTTTGAAGATGTTGACAAGTATTGGCCAACCTATCGGTGTGGTCTTGTAAGCAGTTGTGATAATAGAAAAGGATCATTTTGGGCTCATCAGAAGCATGGAACCTGTGGATCGCCTGTAATCCTACAAGAATATGATTACTTCTTGACAACTCTCACCCTTTTCTACAAGTATAATGTCACTGTAAGCAACCTTTTACTAACTACACTTTATCCTCAATTTGTCTCATTAAGATTGTATCTTTCTTAG

Coding sequence (CDS)

ATGATAAAAGCGGGGAGTAGGAGGGTGGTGTTTGTAGTAGTTGTTGTTATTGGTATTGTTGTTGTTGTTGTTGACGGTGGGGGCTTCACCGTGGGGAAGGAACAGAGGGAATTTGATTACTTTATATTGGCTCTTCAATGGCCTTCCACTTCATGCACAAACGTAACCGTATGTTGCACCACCAACGCTTGCTGCCGCCGAGCTGTCTCTCCCACTGAATTCACAATACATGGGCTGTGGCCTAAGTACGAAGGAAAAGGATGGCCTTCATGTTGTACTAATGAAACCTTCGATGAAACTCAGATTCGTTCCCTGTTTGAAGATGTTGACAAGTATTGGCCAACCTATCGGTGTGGTCTTGTAAGCAGTTGTGATAATAGAAAAGGATCATTTTGGGCTCATCAGAAGCATGGAACCTGTGGATCGCCTGTAATCCTACAAGAATATGATTACTTCTTGACAACTCTCACCCTTTTCTACAAGTATAATGTCACTGTAAGCAACCTTTTACTAACTACACTTTATCCTCAATTTGTCTCATTAAGATTGTATCTTTCTTAG

Protein sequence

MIKAGSRRVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCTTNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGLVSSCDNRKGSFWAHQKHGTCGSPVILQEYDYFLTTLTLFYKYNVTVSNLLLTTLYPQFVSLRLYLS*

Homology

[Close]

BLAST of Cucsa.398180 vs. Swiss-Prot
Match: RNS2_ARATH (Ribonuclease 2 OS=Arabidopsis thaliana GN=RNS2 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.1e-45
Identity = 78/155 (50.32%), Postives = 102/155 (65.81%), Query Frame = 1


Query: 13  VVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCTTNACCRRAVSPT 72
           +++++  +     G    + + QREFDYF L+LQWP T C     CC+ NACCR + +PT
Sbjct: 7   LLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPT 66

Query: 73  EFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGLVSSCDNRKGSFW 132
           +FTIHGLWP Y    WPSCC    F E +I +L + ++KYWP+  CG  SSC+  KGSFW
Sbjct: 67  QFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFW 126

Query: 133 AH--QKHGTCGSPVILQEYDYFLTTLTLFYKYNVT 166
            H  +KHGTC SPV   EY+YFLTTL L+ K+NVT
Sbjct: 127 GHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVT 161

[Close]

BLAST of Cucsa.398180 vs. Swiss-Prot
Match: RNS1_ARATH (Ribonuclease 1 OS=Arabidopsis thaliana GN=RNS1 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 2.7e-17
Identity = 51/149 (34.23%), Postives = 72/149 (48.32%), Query Frame = 1


Query: 19  IVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCTTNACCRRAVSPTEFTIHG 78
           I ++V+    F+      +FD+F    QWP + C     CC  N+         +F IHG
Sbjct: 11  ISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNS----GKPAADFGIHG 70

Query: 79  LWPKYEGKGWPS-CCTNETFDETQIRSLFEDVDKYWPTYRCGLVSSCDNRKGSFWAH--Q 138
           LWP Y+   +PS C  ++ FD + I  L   + K WPT  C   S       +FW H  +
Sbjct: 71  LWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSG-----EAFWEHEWE 130

Query: 139 KHGTCGSPVILQEYDYFLTTLTLFYKYNV 165
           KHGTC   VI  +++YF T L L  K N+
Sbjct: 131 KHGTCSESVI-DQHEYFQTALNLKQKTNL 149

[Close]

BLAST of Cucsa.398180 vs. Swiss-Prot
Match: RNS3_ARATH (Ribonuclease 3 OS=Arabidopsis thaliana GN=RNS3 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 2.7e-17
Identity = 49/132 (37.12%), Postives = 66/132 (50.00%), Query Frame = 1


Query: 36  REFDYFILALQWPSTSCTNVTVCCTTNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNE 95
           ++FD+F   LQWP   C +   CC             +F IHGLWP Y+  GWP  C  +
Sbjct: 20  QDFDFFYFVLQWPGAYCDSRHSCCYPQT----GKPAADFGIHGLWPNYKTGGWPQNCNPD 79

Query: 96  T-FDETQIRSLFEDVDKYWPTYRCGLVSSCDNRKGSFWAH--QKHGTCGSPVILQEYDYF 155
           + FD+ ++  L  D+ + WPT  C    S D  K  FW H  +KHGTC     L ++DYF
Sbjct: 80  SRFDDLRVSDLMSDLQREWPTLSC---PSNDGMK--FWTHEWEKHGTCAESE-LDQHDYF 139

Query: 156 LTTLTLFYKYNV 165
              L L  K N+
Sbjct: 140 EAGLKLKQKANL 141

[Close]

BLAST of Cucsa.398180 vs. Swiss-Prot
Match: RNLX_SOLLC (Intracellular ribonuclease LX OS=Solanum lycopersicum GN=RNALX PE=1 SV=2)

HSP 1 Score: 87.0 bits (214), Expect = 2.3e-16
Identity = 52/167 (31.14%), Postives = 81/167 (48.50%), Query Frame = 1


Query: 1   MIKAGSRRVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCT 60
           M+K+  + ++ ++VV   +V+ V            ++FD+F    QWP++ C     CC 
Sbjct: 1   MMKSQKKLLIKIIVVQCLLVLCVTS----------QDFDFFYFVQQWPASYCDTRRSCCY 60

Query: 61  TNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNE-TFDETQIRSLFEDVDKYWPTYRCG 120
                       +F+IHGLWP Y+   WP  C  E + DE++   L   ++K WP+  C 
Sbjct: 61  PTT----GKPDEDFSIHGLWPNYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLAC- 120

Query: 121 LVSSCDNRKGSFWAHQ--KHGTCGSPVILQEYDYFLTTLTLFYKYNV 165
              S D  K  FW+H+  KHGTC +   L ++ YF T L    K N+
Sbjct: 121 --PSSDGLK--FWSHEWLKHGTCSA---LNQHAYFQTALDFKTKSNL 145

[Close]

BLAST of Cucsa.398180 vs. Swiss-Prot
Match: RNLE_SOLLC (Extracellular ribonuclease LE OS=Solanum lycopersicum PE=1 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 9.7e-15
Identity = 42/126 (33.33%), Postives = 59/126 (46.83%), Query Frame = 1


Query: 36  REFDYFILALQWPSTSCTNVTVCCTTNACCRRAVSPTEFTIHGLWPKYEGKGWPS-CCTN 95
           ++FD+F    QWP + C     CC             +F IHGLWP      +PS C  N
Sbjct: 27  KDFDFFYFVQQWPGSYCDTKQSCCYPTT----GKPAADFGIHGLWPNNNDGTYPSNCDPN 86

Query: 96  ETFDETQIRSLFEDVDKYWPTYRCGLVSSCDNRKGSFWAH--QKHGTCGSPVILQEYDYF 155
             +D++QI  L   + + WPT  C   S       +FW+H  +KHGTC   V+  ++ YF
Sbjct: 87  SPYDQSQISDLISSMQQNWPTLACPSGSG-----STFWSHEWEKHGTCAESVLTNQHAYF 143

Query: 156 LTTLTL 159
              L L
Sbjct: 147 KKALDL 143

[Close]

BLAST of Cucsa.398180 vs. TrEMBL
Match: A0A0A0KVR6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G109040 PE=3 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 3.3e-94
Identity = 165/167 (98.80%), Postives = 165/167 (98.80%), Query Frame = 1


Query: 1   MIKAGSRRVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCT 60
           MIKAGSRRVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCT
Sbjct: 1   MIKAGSRRVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCT 60

Query: 61  TNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGL 120
           TNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGL
Sbjct: 61  TNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGL 120

Query: 121 VSSCDNRKGSFWAHQ--KHGTCGSPVILQEYDYFLTTLTLFYKYNVT 166
           VSSCDNRKGSFWAHQ  KHGTCGSPVILQEYDYFLTTLTLFYKYNVT
Sbjct: 121 VSSCDNRKGSFWAHQYEKHGTCGSPVILQEYDYFLTTLTLFYKYNVT 167

[Close]

BLAST of Cucsa.398180 vs. TrEMBL
Match: A0A0A0L0P9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G109540 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 2.1e-56
Identity = 103/166 (62.05%), Postives = 126/166 (75.90%), Query Frame = 1


Query: 6   SRRVVFVVVVVIGIVVVVVDGGGFTV----GKEQREFDYFILALQWPSTSCTNVTVCCTT 65
           ++ +VFVVVV++ ++ VV    GF+       EQREFDYF+LALQWP +SC N   CC +
Sbjct: 8   AKAMVFVVVVLLQLLTVVF---GFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGKCCPS 67

Query: 66  NACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGLV 125
           NACCR A SPTEFTIHGLWP+Y  KGWPSCCT+ +F+E +I  L ED+ KYWPTYRCG  
Sbjct: 68  NACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYRCGTT 127

Query: 126 SSCDNRKGSFWAH--QKHGTCGSPVILQEYDYFLTTLTLFYKYNVT 166
           S+C   KGSFWAH  +KHGTC +PVI+ EYDYFLTT+T+F KYNVT
Sbjct: 128 STCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVT 170

[Close]

BLAST of Cucsa.398180 vs. TrEMBL
Match: A0A162A2W5_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_018604 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 7.9e-48
Identity = 82/156 (52.56%), Postives = 109/156 (69.87%), Query Frame = 1


Query: 12  VVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCTTNACCRRAVSP 71
           ++V++    +  ++ GGF + KEQREFDYF L+LQWP T C N   CC +N CCR + SP
Sbjct: 15  LIVLIGSFFIEPINAGGFNIVKEQREFDYFKLSLQWPGTVCRNTRHCCASNGCCRGSNSP 74

Query: 72  TEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGLVSSCDNRKGSF 131
           TEFTIHGLWP Y    WP+CCT   FD  +I +L + ++KYWP+Y C   S+C  +KG F
Sbjct: 75  TEFTIHGLWPDYNDGTWPACCTKSGFDVKEISTLKDALEKYWPSYSCSKASTCSGKKGLF 134

Query: 132 WAH--QKHGTCGSPVILQEYDYFLTTLTLFYKYNVT 166
           WAH  +KHGTC SPV+  EY YF+TTL +++K+NVT
Sbjct: 135 WAHEWEKHGTCSSPVVKDEYSYFITTLNVYFKFNVT 170

[Close]

BLAST of Cucsa.398180 vs. TrEMBL
Match: M5W6T6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009963mg PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.0e-46
Identity = 87/160 (54.37%), Postives = 110/160 (68.75%), Query Frame = 1


Query: 9   VVFVVVVVIGIVVVVVDGGGFTVG-KEQREFDYFILALQWPSTSCTNVTVCCTTNACCRR 68
           V  +VV  + ++     G G  +G ++QREFDYF LALQWP T C +   CC++NACCR 
Sbjct: 12  VALIVVASLCLIEANQTGIGIEIGSRKQREFDYFNLALQWPGTFCQHTRHCCSSNACCRG 71

Query: 69  AVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGLVSSCDNR 128
           A SPT FTIHGLWP Y    WP+CCT ++FDE +I +L + ++KYWP+  CG  SSC   
Sbjct: 72  ANSPTVFTIHGLWPDYNDGTWPACCTQKSFDEKEISTLHDALEKYWPSLSCGRPSSCHGG 131

Query: 129 KGSFWAH--QKHGTCGSPVILQEYDYFLTTLTLFYKYNVT 166
           KGSFW H  +KHGTC SPV   EY YFLTTL +++KYNVT
Sbjct: 132 KGSFWGHEWEKHGTCSSPVARDEYSYFLTTLNVYFKYNVT 171

[Close]

BLAST of Cucsa.398180 vs. TrEMBL
Match: D2KKB6_CITRE (S-like RNase OS=Citrus reticulata PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.7e-45
Identity = 87/157 (55.41%), Postives = 104/157 (66.24%), Query Frame = 1


Query: 11  FVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCTTNACCRRAVS 70
           F  +  IGI  VV D  G     EQREFDYF  ALQWP T C N   CC +N CCR + +
Sbjct: 22  FCQLKAIGIREVVDDAVG-----EQREFDYFNFALQWPGTQCKNTRHCCPSNGCCRGSNA 81

Query: 71  PTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGLVSSCDNRKGS 130
           PTEFTIHGLWP Y    WPSCC    FDE +I +L + ++KYWP+YRCG  S+C + +G 
Sbjct: 82  PTEFTIHGLWPDYNDGTWPSCCKKSKFDEKEISTLLDALEKYWPSYRCGSTSTCYSGEGL 141

Query: 131 FWAH--QKHGTCGSPVILQEYDYFLTTLTLFYKYNVT 166
           FWAH  +KHGTC  PV+  EY YF TTL L++KYNVT
Sbjct: 142 FWAHEWEKHGTCSFPVVRDEYSYFSTTLNLYFKYNVT 173

[Close]

BLAST of Cucsa.398180 vs. TAIR10
Match: AT2G39780.1 (AT2G39780.1 ribonuclease 2)

HSP 1 Score: 183.0 bits (463), Expect = 1.7e-46
Identity = 78/155 (50.32%), Postives = 102/155 (65.81%), Query Frame = 1


Query: 13  VVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCTTNACCRRAVSPT 72
           +++++  +     G    + + QREFDYF L+LQWP T C     CC+ NACCR + +PT
Sbjct: 7   LLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPT 66

Query: 73  EFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGLVSSCDNRKGSFW 132
           +FTIHGLWP Y    WPSCC    F E +I +L + ++KYWP+  CG  SSC+  KGSFW
Sbjct: 67  QFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFW 126

Query: 133 AH--QKHGTCGSPVILQEYDYFLTTLTLFYKYNVT 166
            H  +KHGTC SPV   EY+YFLTTL L+ K+NVT
Sbjct: 127 GHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVT 161

[Close]

BLAST of Cucsa.398180 vs. TAIR10
Match: AT1G14220.1 (AT1G14220.1 Ribonuclease T2 family protein)

HSP 1 Score: 99.4 bits (246), Expect = 2.5e-21
Identity = 52/140 (37.14%), Postives = 69/140 (49.29%), Query Frame = 1


Query: 28  GFTVGKEQREFDYFILALQWPSTSCTNVTVCCTTNACCRRAVSPTEFTIHGLWPKYEGKG 87
           G  V + Q +FD+F   LQWP   C     CC   +         +F IHGLWP Y G  
Sbjct: 18  GLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTS----GKPAADFGIHGLWPNYNGGS 77

Query: 88  WPSCCTNET-FDETQIRSLFEDVDKYWPTYRCGLVSSCDNRKGSFWAH--QKHGTCGSPV 147
           WPS C  ++ FD +QI  L   + K WPT  C       N   +FW H  +KHGTC S  
Sbjct: 78  WPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCP-----SNEGFNFWEHEWEKHGTC-SES 137

Query: 148 ILQEYDYFLTTLTLFYKYNV 165
           ++ +++YF   L L  K N+
Sbjct: 138 VMDQHEYFENALKLKQKANL 147

[Close]

BLAST of Cucsa.398180 vs. TAIR10
Match: AT2G02990.1 (AT2G02990.1 ribonuclease 1)

HSP 1 Score: 90.1 bits (222), Expect = 1.5e-18
Identity = 51/149 (34.23%), Postives = 72/149 (48.32%), Query Frame = 1


Query: 19  IVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCTTNACCRRAVSPTEFTIHG 78
           I ++V+    F+      +FD+F    QWP + C     CC  N+         +F IHG
Sbjct: 11  ISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNS----GKPAADFGIHG 70

Query: 79  LWPKYEGKGWPS-CCTNETFDETQIRSLFEDVDKYWPTYRCGLVSSCDNRKGSFWAH--Q 138
           LWP Y+   +PS C  ++ FD + I  L   + K WPT  C   S       +FW H  +
Sbjct: 71  LWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSG-----EAFWEHEWE 130

Query: 139 KHGTCGSPVILQEYDYFLTTLTLFYKYNV 165
           KHGTC   VI  +++YF T L L  K N+
Sbjct: 131 KHGTCSESVI-DQHEYFQTALNLKQKTNL 149

[Close]

BLAST of Cucsa.398180 vs. TAIR10
Match: AT1G26820.1 (AT1G26820.1 ribonuclease 3)

HSP 1 Score: 90.1 bits (222), Expect = 1.5e-18
Identity = 49/132 (37.12%), Postives = 66/132 (50.00%), Query Frame = 1


Query: 36  REFDYFILALQWPSTSCTNVTVCCTTNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNE 95
           ++FD+F   LQWP   C +   CC             +F IHGLWP Y+  GWP  C  +
Sbjct: 20  QDFDFFYFVLQWPGAYCDSRHSCCYPQT----GKPAADFGIHGLWPNYKTGGWPQNCNPD 79

Query: 96  T-FDETQIRSLFEDVDKYWPTYRCGLVSSCDNRKGSFWAH--QKHGTCGSPVILQEYDYF 155
           + FD+ ++  L  D+ + WPT  C    S D  K  FW H  +KHGTC     L ++DYF
Sbjct: 80  SRFDDLRVSDLMSDLQREWPTLSC---PSNDGMK--FWTHEWEKHGTCAESE-LDQHDYF 139

Query: 156 LTTLTLFYKYNV 165
              L L  K N+
Sbjct: 140 EAGLKLKQKANL 141

[Close]

BLAST of Cucsa.398180 vs. NCBI nr
Match: gi|778691966|ref|XP_011653385.1| (PREDICTED: ribonuclease 2-like [Cucumis sativus])

HSP 1 Score: 352.4 bits (903), Expect = 4.7e-94
Identity = 165/167 (98.80%), Postives = 165/167 (98.80%), Query Frame = 1


Query: 1   MIKAGSRRVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCT 60
           MIKAGSRRVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCT
Sbjct: 1   MIKAGSRRVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCT 60

Query: 61  TNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGL 120
           TNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGL
Sbjct: 61  TNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGL 120

Query: 121 VSSCDNRKGSFWAHQ--KHGTCGSPVILQEYDYFLTTLTLFYKYNVT 166
           VSSCDNRKGSFWAHQ  KHGTCGSPVILQEYDYFLTTLTLFYKYNVT
Sbjct: 121 VSSCDNRKGSFWAHQYEKHGTCGSPVILQEYDYFLTTLTLFYKYNVT 167

[Close]

BLAST of Cucsa.398180 vs. NCBI nr
Match: gi|700198557|gb|KGN53715.1| (hypothetical protein Csa_4G109040 [Cucumis sativus])

HSP 1 Score: 352.4 bits (903), Expect = 4.7e-94
Identity = 165/167 (98.80%), Postives = 165/167 (98.80%), Query Frame = 1


Query: 1   MIKAGSRRVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCT 60
           MIKAGSRRVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCT
Sbjct: 1   MIKAGSRRVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCT 60

Query: 61  TNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGL 120
           TNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGL
Sbjct: 61  TNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGL 120

Query: 121 VSSCDNRKGSFWAHQ--KHGTCGSPVILQEYDYFLTTLTLFYKYNVT 166
           VSSCDNRKGSFWAHQ  KHGTCGSPVILQEYDYFLTTLTLFYKYNVT
Sbjct: 121 VSSCDNRKGSFWAHQYEKHGTCGSPVILQEYDYFLTTLTLFYKYNVT 167

[Close]

BLAST of Cucsa.398180 vs. NCBI nr
Match: gi|659125796|ref|XP_008462864.1| (PREDICTED: ribonuclease 2-like isoform X3 [Cucumis melo])

HSP 1 Score: 305.1 bits (780), Expect = 8.6e-80
Identity = 146/169 (86.39%), Postives = 153/169 (90.53%), Query Frame = 1


Query: 1   MIKAGSR--RVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVC 60
           M+KAGSR  RVVFVVVVVIGI+V   DG GFTVGKEQREFDYF+LALQWPST C NVT C
Sbjct: 1   MLKAGSRSRRVVFVVVVVIGIIV---DGWGFTVGKEQREFDYFVLALQWPSTLCRNVTEC 60

Query: 61  CTTNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRC 120
           C TNAC RRAVS T+FTIHGLWPKYEGKGWPSCCTN TF++TQI SLFEDVDKYWPTYRC
Sbjct: 61  CPTNACYRRAVSATKFTIHGLWPKYEGKGWPSCCTNATFNQTQIGSLFEDVDKYWPTYRC 120

Query: 121 GLVSSCDNRKGSFWAHQ--KHGTCGSPVILQEYDYFLTTLTLFYKYNVT 166
           GLVSSCDNRKGSFWAHQ  KHGTCGSPVILQEYDYFLTTLTLF+KYNVT
Sbjct: 121 GLVSSCDNRKGSFWAHQYEKHGTCGSPVILQEYDYFLTTLTLFFKYNVT 166

[Close]

BLAST of Cucsa.398180 vs. NCBI nr
Match: gi|659125792|ref|XP_008462862.1| (PREDICTED: ribonuclease 2-like isoform X1 [Cucumis melo])

HSP 1 Score: 305.1 bits (780), Expect = 8.6e-80
Identity = 146/169 (86.39%), Postives = 153/169 (90.53%), Query Frame = 1


Query: 1   MIKAGSR--RVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVC 60
           M+KAGSR  RVVFVVVVVIGI+V   DG GFTVGKEQREFDYF+LALQWPST C NVT C
Sbjct: 1   MLKAGSRSRRVVFVVVVVIGIIV---DGWGFTVGKEQREFDYFVLALQWPSTLCRNVTEC 60

Query: 61  CTTNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRC 120
           C TNAC RRAVS T+FTIHGLWPKYEGKGWPSCCTN TF++TQI SLFEDVDKYWPTYRC
Sbjct: 61  CPTNACYRRAVSATKFTIHGLWPKYEGKGWPSCCTNATFNQTQIGSLFEDVDKYWPTYRC 120

Query: 121 GLVSSCDNRKGSFWAHQ--KHGTCGSPVILQEYDYFLTTLTLFYKYNVT 166
           GLVSSCDNRKGSFWAHQ  KHGTCGSPVILQEYDYFLTTLTLF+KYNVT
Sbjct: 121 GLVSSCDNRKGSFWAHQYEKHGTCGSPVILQEYDYFLTTLTLFFKYNVT 166

[Close]

BLAST of Cucsa.398180 vs. NCBI nr
Match: gi|659125798|ref|XP_008462865.1| (PREDICTED: ribonuclease 2-like isoform X4 [Cucumis melo])

HSP 1 Score: 305.1 bits (780), Expect = 8.6e-80
Identity = 146/169 (86.39%), Postives = 153/169 (90.53%), Query Frame = 1


Query: 1   MIKAGSR--RVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVC 60
           M+KAGSR  RVVFVVVVVIGI+V   DG GFTVGKEQREFDYF+LALQWPST C NVT C
Sbjct: 1   MLKAGSRSRRVVFVVVVVIGIIV---DGWGFTVGKEQREFDYFVLALQWPSTLCRNVTEC 60

Query: 61  CTTNACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRC 120
           C TNAC RRAVS T+FTIHGLWPKYEGKGWPSCCTN TF++TQI SLFEDVDKYWPTYRC
Sbjct: 61  CPTNACYRRAVSATKFTIHGLWPKYEGKGWPSCCTNATFNQTQIGSLFEDVDKYWPTYRC 120

Query: 121 GLVSSCDNRKGSFWAHQ--KHGTCGSPVILQEYDYFLTTLTLFYKYNVT 166
           GLVSSCDNRKGSFWAHQ  KHGTCGSPVILQEYDYFLTTLTLF+KYNVT
Sbjct: 121 GLVSSCDNRKGSFWAHQYEKHGTCGSPVILQEYDYFLTTLTLFFKYNVT 166

The following BLAST results are available for this feature:

BLAST of Cucsa.398180 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RNS2_ARATH	3.1e-45	50.32	Ribonuclease 2 OS=Arabidopsis thaliana GN=RNS2 PE=2 SV=1	[more]
RNS1_ARATH	2.7e-17	34.23	Ribonuclease 1 OS=Arabidopsis thaliana GN=RNS1 PE=1 SV=1	[more]
RNS3_ARATH	2.7e-17	37.12	Ribonuclease 3 OS=Arabidopsis thaliana GN=RNS3 PE=2 SV=1	[more]
RNLX_SOLLC	2.3e-16	31.14	Intracellular ribonuclease LX OS=Solanum lycopersicum GN=RNALX PE=1 SV=2	[more]
RNLE_SOLLC	9.7e-15	33.33	Extracellular ribonuclease LE OS=Solanum lycopersicum PE=1 SV=2	[more]

BLAST of Cucsa.398180 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KVR6_CUCSA	3.3e-94	98.80	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G109040 PE=3 SV=1	[more]
A0A0A0L0P9_CUCSA	2.1e-56	62.05	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G109540 PE=3 SV=1	[more]
A0A162A2W5_DAUCA	7.9e-48	52.56	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_018604 PE=4 SV=1	[more]
M5W6T6_PRUPE	2.0e-46	54.38	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009963mg PE=3 SV=1	[more]
D2KKB6_CITRE	1.7e-45	55.41	S-like RNase OS=Citrus reticulata PE=2 SV=1	[more]

BLAST of Cucsa.398180 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G39780.1	1.7e-46	50.32	ribonuclease 2	[more]
AT1G14220.1	2.5e-21	37.14	Ribonuclease T2 family protein	[more]
AT2G02990.1	1.5e-18	34.23	ribonuclease 1	[more]
AT1G26820.1	1.5e-18	37.12	ribonuclease 3	[more]

BLAST of Cucsa.398180 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778691966\|ref\|XP_011653385.1\|	4.7e-94	98.80	PREDICTED: ribonuclease 2-like [Cucumis sativus]	[more]
gi\|700198557\|gb\|KGN53715.1\|	4.7e-94	98.80	hypothetical protein Csa_4G109040 [Cucumis sativus]	[more]
gi\|659125796\|ref\|XP_008462864.1\|	8.6e-80	86.39	PREDICTED: ribonuclease 2-like isoform X3 [Cucumis melo]	[more]
gi\|659125792\|ref\|XP_008462862.1\|	8.6e-80	86.39	PREDICTED: ribonuclease 2-like isoform X1 [Cucumis melo]	[more]
gi\|659125798\|ref\|XP_008462865.1\|	8.6e-80	86.39	PREDICTED: ribonuclease 2-like isoform X4 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001568	RNase_T2-like
IPR018188	RNase_T2_His_AS_1

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0033897	ribonuclease T2 activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044248	cellular catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0008150	biological_process
cellular_component	GO:0044444	cytoplasmic part
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.398180.1	Cucsa.398180.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	GENE3D	G3DSA:3.90.730.10		coord: 35..165 score: 4.8
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 1..165 score: 1.4
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 40..165 score: 4.1
IPR001568	Ribonuclease T2-like	unknown	SSF55895	Ribonuclease Rh-like	coord: 37..165 score: 2.75
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 74..81 scor
None	No IPR available	PANTHER	PTHR11240:SF16	RIBONUCLEASE 2	coord: 1..165 score: 1.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.398180	Cla002480	Watermelon (97103) v1	cgywmB714
Cucsa.398180	Csa4G109040	Cucumber (Chinese Long) v2	cgycuB556
Cucsa.398180	MELO3C024816	Melon (DHL92) v3.5.1	cgymeB653
Cucsa.398180	ClCG10G009300	Watermelon (Charleston Gray)	cgywcgB678
Cucsa.398180	CSPI04G09320	Wild cucumber (PI 183967)	cgycpiB585
Cucsa.398180	CmaCh16G002600	Cucurbita maxima (Rimu)	cgycmaB1101
Cucsa.398180	CmoCh16G002670	Cucurbita moschata (Rifu)	cgycmoB1100
Cucsa.398180	Cp4.1LG14g05080	Cucurbita pepo (Zucchini)	cgycpeB1052
Cucsa.398180	MELO3C024816.2	Melon (DHL92) v3.6.1	cgymedB649
Cucsa.398180	CsaV3_4G009480	Cucumber (Chinese Long) v3	cgycucB604
Cucsa.398180	Cla97C10G192460	Watermelon (97103) v2	cgywmbB680
Cucsa.398180	Carg16789	Silver-seed gourd	carcgyB0994

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.398180	Silver-seed gourd	carcgyB0441
Cucsa.398180	Wax gourd	cgywgoB792
Cucsa.398180	Cucurbita maxima (Rimu)	cgycmaB1102
Cucsa.398180	Cucurbita moschata (Rifu)	cgycmoB1101
Cucsa.398180	Cucurbita pepo (Zucchini)	cgycpeB1053
Cucsa.398180	Bottle gourd (USVL1VR-Ls)	cgylsiB641