Cucsa.352210 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.352210
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionProtein DCL, chloroplastic
Locationscaffold03533 : 124085 .. 126664 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAGAGAATGCGGATTGAAGAGAAATCCTGTAGAATCTCCAATCCATGAGATTGATGATAATGTGAGCGACCGTTAACTGTTCTTCTTCCTCCGTCTTCTCTAACAATTTGGGAGAAAAGCCATGCTCCGAAATCGCACACTACCATTTCATTCTCAGTAGTTGCAAATTCAAGCTCCTTGTTTCGATTGATCAAGGGCATGGCCATGGCGTCCATTTTGAAACCACCGCCATTTCCGCCCTTTCACTCTCTAAATCCAAACTTCTTCAACTCTTCACCACTGATCTTATGTTTTCCTACACACCCAATCAATTCGTTTCACCCATCTACTCGTGCCCTCAAAACTGGCCCTGAAGGCATTAGAATTCGAAGCCATCAAGAATATAGTTCCGATTTGCTGCGGAAACCGGTTGGTCCATCGGCGAAGGACTTGGCTGGACCATCGGAGGATGACGACAGCAGTGAGGAGAGTGGAAATGAAGACGAGGAGGAAGTGGAGTGGGTTGATTGGGAGGACAAGATTTTGGAGGATACTGTTCCTCTAGTTGGCTTTGTTAGGATGGTTCTTCACACTGGAAAGTAAGCAACCATATTCTTAGTGAATTTTTAACTGAATTTGAACTTCCTGGTCAAATAGAAATTATGCAACTCTATTCTTAGTGAATTTTTAACTTGCGTTTGTTCAACTGGAAACGTGTCACTTGAAGATGAACGGAACTATATAATTTGAGATTGAAGTTACGGAACTTCTACGGAATGTTCATCAGTTTCCATAGCACAAGAACAAGAATCACGAGTACCGTCAATTAATCTCTTCGAAGTTCATTGATTTTATTCAATTTTTTGGAATTTGATTAATAATTGGAATACCCAAAGACTTTGACTCTAAAAAATTGATGAAAAAATGGCTCTTGCAACAGTACATACTCAACCTATGTCCCTTAGCCCATGGATATTGCTTGCAGTACTCGCAATATTATCTTTATGAAAAAGGGTTTCACAATATGAATGATTAAGATTTCTATCTCTCTCAAAAAGATATGTAGTCTAAGCCAGTAGTCAAGTCAATATATCTTCTCTCTCTTACTCATCTGATTAGGTGTTCGTAGCATGTATGCTGTTATCTCTTAGTAAAAGTTTCAAGTTGCTATTATTTCAGCATATCTCCTCAGAGACAAAATTATAGCGTTTTGTAGTTGTTTTTCTTCCCTTGGATGTATGAGATATCATGACTGTTGGTCTTGCAATTACAATGGTGGATAAAAAATCTGAAAGAGAATATTTTGTGTCTTGGAGTCATTAGCATGATGAAGTAGAGTTTAATCTCCATTCGTTAATCATCTTTAATCATACACTTGCAGATATGAAAATGGGGATAGGTTGAGACCAGAGCACGAGAAAACAATTCTTGAGAGGTTGCTTCCATATCATCCCGAGAGTGAGAAGAAGATTGGATGTGGGGTTGATTATATCACGGTATATCTCTCTCCATGCATATTTGTACATGTCATGTGTTTTGAGCTTATTTTTATAATTTACATTTATGTAAAATAAAACAACTAATTAGTACTCTAGCCTGTAGCCTTTTTTTCTATTTCCTTGGACGAAAGAAAGCTCGTCTTGAACCTGGTAAAGAAATTAAATGTTCACAATCAAGTTTTTTATTACTTCTGATGAAGCATGCTGATTATCGCTGTTGATGAGATCAACTTCAATCAGTCCATGATCTGTACCGTGGATGTTTTTACAAGCTTAGTTTGTAATGCTAAAATATTTGTCTGTACTGTTCAACTTGTAAAGACATAAATTGTTGTGGAACAGAACACTTCTAAGAAAGGAAAACTTTCTTTTCCTTCTACTTGTTATGTACATTAACTGTGGATTTTTTTTtCTTTCTAAAAGACGTGTTTCCTGTGATATAAACGAAGAATGCATAAGAGCGAGAAGGCATTGATAAGTGGCAACGGTTTGAATTTTCACTTGATTGTATATATGTGATGCTGCCGAAAGGGTTATGCCTTGACTTGAAAAaCCTGCTTATGGGCAGTTCCATTTTCGTTTTTtGAACTATTTTCTTTGGAATTATACTGGGCATTGGATATACGCTGTATTTGTCAACTGAAATGATCATGGCAATTTTTTATTGGTGGTGTTGTGGTCGGCAGGTTGGATATCATCCTGATTTTGAAAGCTCAAGATGTTTATTCATAGTCAGAAAAGATGGAGAGATGGTTGACTTTTCATATTGGAAGTGCATCAAGGGTCTGATCAGAAAGAATTATCCTCTTTACGCAGAAAGCTTCATTCTTAGGCATTTTCGGCGACGTAGACGCAGTTCCCGTCGGTAACACATGTAAGATCTGTTAATCATACTCTTGTATCATTCAATTTCAGGAATTCTTTGAATATCAAAGCTTGAGATTGGACCAGCCACGCTTGTGTGGTTCTCTTGTGTTTAATGACTTGAAATTAACAAATCAAGATGGATATGGGGTGAATATAGACTCTTGCTTAGTAAAATTCAATTTTGTAAATTCCAACAGTTGCCTCCAGTTTTCCTTGATCCAATTCATGCTTAGT

mRNA sequence

CCAGAGAATGCGGATTGAAGAGAAATCCTGTAGAATCTCCAATCCATGAGATTGATGATAATGTGAGCGACCGTTAACTGTTCTTCTTCCTCCGTCTTCTCTAACAATTTGGGAGAAAAGCCATGCTCCGAAATCGCACACTACCATTTCATTCTCAGTAGTTGCAAATTCAAGCTCCTTGTTTCGATTGATCAAGGGCATGGCCATGGCGTCCATTTTGAAACCACCGCCATTTCCGCCCTTTCACTCTCTAAATCCAAACTTCTTCAACTCTTCACCACTGATCTTATGTTTTCCTACACACCCAATCAATTCGTTTCACCCATCTACTCGTGCCCTCAAAACTGGCCCTGAAGGCATTAGAATTCGAAGCCATCAAGAATATAGTTCCGATTTGCTGCGGAAACCGGTTGGTCCATCGGCGAAGGACTTGGCTGGACCATCGGAGGATGACGACAGCAGTGAGGAGAGTGGAAATGAAGACGAGGAGGAAGTGGAGTGGGTTGATTGGGAGGACAAGATTTTGGAGGATACTGTTCCTCTAGTTGGCTTTGTTAGGATGGTTCTTCACACTGGAAAATATGAAAATGGGGATAGGTTGAGACCAGAGCACGAGAAAACAATTCTTGAGAGGTTGCTTCCATATCATCCCGAGAGTGAGAAGAAGATTGGATGTGGGGTTGATTATATCACGGTTGGATATCATCCTGATTTTGAAAGCTCAAGATGTTTATTCATAGTCAGAAAAGATGGAGAGATGGTTGACTTTTCATATTGGAAGTGCATCAAGGGTCTGATCAGAAAGAATTATCCTCTTTACGCAGAAAGCTTCATTCTTAGGCATTTTCGGCGACGTAGACGCAGTTCCCGTCGGTAACACATGTAAGATCTGTTAATCATACTCTTGTATCATTCAATTTCAGGAATTCTTTGAATATCAAAGCTTGAGATTGGACCAGCCACGCTTGTGTGGTTCTCTTGTGTTTAATGACTTGAAATTAACAAATCAAGATGGATATGGGGTGAATATAGACTCTTGCTTAGTAAAATTCAATTTTGTAAATTCCAACAGTTGCCTCCAGTTTTCCTTGATCCAATTCATGCTTAGT

Coding sequence (CDS)

ATGGCCATGGCGTCCATTTTGAAACCACCGCCATTTCCGCCCTTTCACTCTCTAAATCCAAACTTCTTCAACTCTTCACCACTGATCTTATGTTTTCCTACACACCCAATCAATTCGTTTCACCCATCTACTCGTGCCCTCAAAACTGGCCCTGAAGGCATTAGAATTCGAAGCCATCAAGAATATAGTTCCGATTTGCTGCGGAAACCGGTTGGTCCATCGGCGAAGGACTTGGCTGGACCATCGGAGGATGACGACAGCAGTGAGGAGAGTGGAAATGAAGACGAGGAGGAAGTGGAGTGGGTTGATTGGGAGGACAAGATTTTGGAGGATACTGTTCCTCTAGTTGGCTTTGTTAGGATGGTTCTTCACACTGGAAAATATGAAAATGGGGATAGGTTGAGACCAGAGCACGAGAAAACAATTCTTGAGAGGTTGCTTCCATATCATCCCGAGAGTGAGAAGAAGATTGGATGTGGGGTTGATTATATCACGGTTGGATATCATCCTGATTTTGAAAGCTCAAGATGTTTATTCATAGTCAGAAAAGATGGAGAGATGGTTGACTTTTCATATTGGAAGTGCATCAAGGGTCTGATCAGAAAGAATTATCCTCTTTACGCAGAAAGCTTCATTCTTAGGCATTTTCGGCGACGTAGACGCAGTTCCCGTCGGTAA

Protein sequence

MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRIRSHQEYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVRMVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFIVRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRSSRR*
Homology
[Close]
BLAST of Cucsa.352210 vs. Swiss-Prot
Match: DCL_SOLLC (Protein DCL, chloroplastic OS=Solanum lycopersicum GN=DCL PE=2 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 7.8e-67
Identity = 124/180 (68.89%), Postives = 148/180 (82.22%), Query Frame = 1 

Query: 46  ALKTGPEGIRIRSHQEYSSDLLRKPVGPSAKDLAGPSED---DDSSEESGNEDEEEVEWV 105
           A+KTG EG  IRS    +++LLRKPV  +  +    SE+   ++S +E G +  +   WV
Sbjct: 47  AVKTGSEGGGIRSD---NAELLRKPVISTELETTSESEELVKEESDDEVGKKSGDGEGWV 106

Query: 106 DWEDKILEDTVPLVGFVRMVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVD 165
           DWED+ILEDTVPLVGFVRM+LH+GKY  GDRL P+H++TIL+RLLPYHPE +KKIG GVD
Sbjct: 107 DWEDQILEDTVPLVGFVRMILHSGKYAIGDRLSPDHQRTILQRLLPYHPECDKKIGPGVD 166

Query: 166 YITVGYHPDFESSRCLFIVRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRS 223
           YITVGYHPDFE+SRCLFIVRKDGE VDFSYWKCIKGLIRKNYPLYA+SFILRHFR+RRR+
Sbjct: 167 YITVGYHPDFENSRCLFIVRKDGETVDFSYWKCIKGLIRKNYPLYADSFILRHFRKRRRN 223

[Close]
BLAST of Cucsa.352210 vs. Swiss-Prot
Match: NRPE1_ARATH (DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana GN=NRPE1 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.2e-21
Identity = 48/121 (39.67%), Postives = 72/121 (59.50%), Query Frame = 1 

Query: 105  EDKILEDTVPLVGFVRMVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYI 164
            E ++L D  P++  +R ++H   Y +GD +  + +  +LE++L +HP+ E K+G GVD+I
Sbjct: 1736 EQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDFI 1795

Query: 165  TVGYHPDFESSRCLFIVRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRSSR 224
            TV  H  F  SRC F+V  DG   DFSY K +   + K YP  AE FI ++F + R S  
Sbjct: 1796 TVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTKPRPSGN 1855

Query: 225  R 226
            R
Sbjct: 1856 R 1856

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BLAST of Cucsa.352210 vs. TrEMBL
Match: A0A0A0L2D0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G639820 PE=4 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 4.5e-130
Identity = 223/225 (99.11%), Postives = 225/225 (100.00%), Query Frame = 1 

Query: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRIRSHQ 60
           MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIR+RSHQ
Sbjct: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRLRSHQ 60

Query: 61  EYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVR 120

Query: 121 MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180
           MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 181 VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRSSRR 226
           VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRR+SRR
Sbjct: 181 VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNSRR 225

[Close]
BLAST of Cucsa.352210 vs. TrEMBL
Match: A0A061FBM3_THECC (Uncharacterized protein isoform 1 OS=Theobroma cacao GN=TCM_033826 PE=4 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 5.2e-78
Identity = 144/220 (65.45%), Postives = 175/220 (79.55%), Query Frame = 1 

Query: 3   MASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRIRSHQEY 62
           MAS+LKPPP+   + ++ +  +SSP+IL  P+    S    + AL+TG +G RI S + Y
Sbjct: 1   MASVLKPPPYFHRNCISISS-SSSPVILSSPSQRTTSLQVRSCALRTGSDGGRIGSQESY 60

Query: 63  SSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVRMV 122
            +D+LRKP   + KD  G SE ++ SE       +  +W+DWED+ILEDTVPLVGFVRM+
Sbjct: 61  GADMLRKPSILTPKDSGGTSEQEEGSEGK----RKRGKWIDWEDRILEDTVPLVGFVRMI 120

Query: 123 LHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFIVR 182
           +H+GKYE+GDRL PEHEKTIL+RLLPYHPE EKKIGCG+DYITVGYHPDFE SRCLFIVR
Sbjct: 121 IHSGKYESGDRLSPEHEKTILDRLLPYHPECEKKIGCGIDYITVGYHPDFEGSRCLFIVR 180

Query: 183 KDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRS 223
           KDGE++DFSYWKCIKGLIRKNYPLYA+SFILRHFRRRRRS
Sbjct: 181 KDGELIDFSYWKCIKGLIRKNYPLYADSFILRHFRRRRRS 215

[Close]
BLAST of Cucsa.352210 vs. TrEMBL
Match: B9R7B7_RICCO (DCL protein, chloroplast, putative OS=Ricinus communis GN=RCOM_1590270 PE=4 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 4.4e-77
Identity = 149/224 (66.52%), Postives = 177/224 (79.02%), Query Frame = 1 

Query: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTH-PINSFHPSTRALKTGPEGIRIRSH 60
           M MAS+ KPPP    H  NP     SP+IL FP H    SF+    ALKTG +G      
Sbjct: 1   MTMASLSKPPPCLHGHYSNPISLYFSPVILSFPFHRTTTSFNSRIFALKTGSDG------ 60

Query: 61  QEYSSDLLRKPVGPSAKDLAGPSEDD-DSSEESGNEDEEEVEWVDWEDKILEDTVPLVGF 120
               SDLLRKP+ PS K+L+G S+D+ DS+ +  N+D+E+ E VDWED+ILEDTVPLVGF
Sbjct: 61  ----SDLLRKPIVPSEKELSGISDDEEDSNRKRDNKDKEDDELVDWEDQILEDTVPLVGF 120

Query: 121 VRMVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCL 180
           VRM+LH+GKYENGDRL PEHE+TI+ERLLP+HPE EKKIG G+DYITVG+H +FE+SRCL
Sbjct: 121 VRMILHSGKYENGDRLSPEHERTIVERLLPFHPECEKKIGPGIDYITVGHHTEFENSRCL 180

Query: 181 FIVRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRS 223
           FIVRKDG++VDFSYWKCIKGLIRKNYPLYA+SFILRHFRRRR+S
Sbjct: 181 FIVRKDGKLVDFSYWKCIKGLIRKNYPLYADSFILRHFRRRRQS 214

[Close]
BLAST of Cucsa.352210 vs. TrEMBL
Match: A0A0D2R0L6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G020200 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 7.5e-77
Identity = 146/221 (66.06%), Postives = 167/221 (75.57%), Query Frame = 1 

Query: 3   MASILKPPPFPPFHSLN-PNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRIRSHQE 62
           MASIL PPP P FH  + P   +SSP+IL +P     S     +AL+T  +G +I S + 
Sbjct: 1   MASILNPPPPPYFHRTSMPTSVSSSPVILSYPFLKTTSLQVRFKALRTWSDGGKIGSQEA 60

Query: 63  YSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVRM 122
           Y +D LRKP     KD  G  E+++ SE   N  E    W DWED+ILEDTVPLVGFVRM
Sbjct: 61  YGADFLRKPSTVPKKDSDGILEEEEGSEGKRNRGE----WTDWEDRILEDTVPLVGFVRM 120

Query: 123 VLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFIV 182
           ++H+GKY  GDRL PEHE+TILERLLPYHPE EKKIGCG+DYITVGYHPDF  SRCLFIV
Sbjct: 121 IIHSGKYGAGDRLSPEHERTILERLLPYHPEFEKKIGCGIDYITVGYHPDFVGSRCLFIV 180

Query: 183 RKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRS 223
           RKDGE+VDFSYWKCIKGLIRKNYPLYA+SFILRHFRRRRRS
Sbjct: 181 RKDGELVDFSYWKCIKGLIRKNYPLYADSFILRHFRRRRRS 217

[Close]
BLAST of Cucsa.352210 vs. TrEMBL
Match: A0A0B0NSM0_GOSAR (Protein DCL, chloroplastic OS=Gossypium arboreum GN=F383_03623 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 9.8e-77
Identity = 146/221 (66.06%), Postives = 167/221 (75.57%), Query Frame = 1 

Query: 3   MASILKPPPFPPFHSLN-PNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRIRSHQE 62
           MASIL PPP P FH  + P   +SSP+IL +P     S     +AL+T  +G +I S + 
Sbjct: 1   MASILNPPPPPYFHLTSLPTSVSSSPVILSYPFLKTTSLQVRFKALRTWSDGGKIGSQEA 60

Query: 63  YSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVRM 122
           Y +D LRKP     KD  G  E+++ SE   N  E    W DWED+ILEDTVPLVGFVRM
Sbjct: 61  YGADFLRKPSTVPKKDSDGILEEEEGSEGKRNRGE----WTDWEDRILEDTVPLVGFVRM 120

Query: 123 VLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFIV 182
           ++H+GKY  GDRL PEHE+TILERLLPYHPE EKKIGCG+DYITVGYHPDF  SRCLFIV
Sbjct: 121 IIHSGKYGAGDRLSPEHERTILERLLPYHPEFEKKIGCGIDYITVGYHPDFVGSRCLFIV 180

Query: 183 RKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRS 223
           RKDGE+VDFSYWKCIKGLIRKNYPLYA+SFILRHFRRRRRS
Sbjct: 181 RKDGELVDFSYWKCIKGLIRKNYPLYADSFILRHFRRRRRS 217

[Close]
BLAST of Cucsa.352210 vs. TAIR10
Match: AT1G45230.1 (AT1G45230.1 Protein of unknown function (DUF3223))

HSP 1 Score: 268.9 bits (686), Expect = 2.9e-72
Identity = 137/225 (60.89%), Postives = 167/225 (74.22%), Query Frame = 1 

Query: 1   MAMASILKPPPF--PPFHSLNPNF-FNSSPLILCFPTHPINSFHPSTRALKTGPEGIRIR 60
           M++ASI    P   P F      F F+SSPL L FP     S  P  RAL+T  +G +I 
Sbjct: 1   MSLASIPSSSPVASPYFRCRTYIFSFSSSPLCLYFPRGDSTSLRPRVRALRTESDGAKIG 60

Query: 61  SHQEYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVG 120
           + + Y S+LLR+P   S +     SE+++  EE  +E +E   +VDWEDKILE TVPLVG
Sbjct: 61  NSESYGSELLRRPRIASEES----SEEEEEEEEENSEGDE---FVDWEDKILEVTVPLVG 120

Query: 121 FVRMVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRC 180
           FVRM+LH+GKY N DRL PEHE+TI+E LLPYHPE EKKIGCG+DYI VG+HPDFESSRC
Sbjct: 121 FVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRC 180

Query: 181 LFIVRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRS 223
           +FIVRKDGE+VDFSYWKCIKGLI+K YPLYA+SFILRHFR+RR++
Sbjct: 181 MFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRKRRQN 218

[Close]
BLAST of Cucsa.352210 vs. TAIR10
Match: AT3G46630.1 (AT3G46630.1 Protein of unknown function (DUF3223))

HSP 1 Score: 136.3 bits (342), Expect = 2.3e-32
Identity = 61/125 (48.80%), Postives = 85/125 (68.00%), Query Frame = 1 

Query: 94  EDEEEVEWVDWEDKILEDTVPLVGFVRMVLHTGKYENGDRLRPEHEKTILERLLPYHPES 153
           ED +  +W + E +IL D  P+    + +LH+ +Y +G+RL  E EK ++E+LLPYHP S
Sbjct: 80  EDPDYRKWKNLEAEILRDIEPISLLAKEILHSDRYLDGERLDFEDEKIVMEKLLPYHPYS 139

Query: 154 EKKIGCGVDYITVGYHPDFESSRCLFIVRKDGEMVDFSYWKCIKGLIRKNYPLYAESFIL 213
           + KIGCG+D+I V  HP F  SRCLF+VR DG  +DFSY KC++  +R  YP +AE FI 
Sbjct: 140 KDKIGCGLDFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYVRDKYPSHAERFIR 199

Query: 214 RHFRR 219
            HF+R
Sbjct: 200 EHFKR 204

[Close]
BLAST of Cucsa.352210 vs. TAIR10
Match: AT2G40030.1 (AT2G40030.1 nuclear RNA polymerase D1B)

HSP 1 Score: 104.0 bits (258), Expect = 1.2e-22
Identity = 48/121 (39.67%), Postives = 72/121 (59.50%), Query Frame = 1 

Query: 105  EDKILEDTVPLVGFVRMVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYI 164
            E ++L D  P++  +R ++H   Y +GD +  + +  +LE++L +HP+ E K+G GVD+I
Sbjct: 1736 EQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDFI 1795

Query: 165  TVGYHPDFESSRCLFIVRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRSSR 224
            TV  H  F  SRC F+V  DG   DFSY K +   + K YP  AE FI ++F + R S  
Sbjct: 1796 TVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTKPRPSGN 1855

Query: 225  R 226
            R
Sbjct: 1856 R 1856

[Close]
BLAST of Cucsa.352210 vs. TAIR10
Match: AT5G62440.1 (AT5G62440.1 Protein of unknown function (DUF3223))

HSP 1 Score: 48.5 bits (114), Expect = 6.2e-06
Identity = 39/129 (30.23%), Postives = 54/129 (41.86%), Query Frame = 1 

Query: 72  GPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVRMVLHTGKYENG 131
           G S K   G  E     +    E    V   D+  K L        F    L   KYE+ 
Sbjct: 51  GESKKQKVGEEEKSGPVKLGPKEFVTSVAMFDYFVKFLH-------FWPTDLDVNKYEH- 110

Query: 132 DRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFIVRKDGEMVDFS 191
                     +L+ +   H E EKKIG G+    V  HP ++S RC F+VR+D    DFS
Sbjct: 111 --------MVLLDLIKKGHSEPEKKIGGGIKTFQVRTHPMWKS-RCFFLVREDDTADDFS 162

Query: 192 YWKCIKGLI 201
           + KC+  ++
Sbjct: 171 FRKCVDQIL 162

[Close]
BLAST of Cucsa.352210 vs. NCBI nr
Match: gi|449447003|ref|XP_004141259.1| (PREDICTED: protein DCL, chloroplastic [Cucumis sativus])

HSP 1 Score: 471.9 bits (1213), Expect = 6.5e-130
Identity = 223/225 (99.11%), Postives = 225/225 (100.00%), Query Frame = 1 

Query: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRIRSHQ 60
           MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIR+RSHQ
Sbjct: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRLRSHQ 60

Query: 61  EYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVR 120

Query: 121 MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180
           MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 181 VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRSSRR 226
           VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRR+SRR
Sbjct: 181 VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNSRR 225

[Close]
BLAST of Cucsa.352210 vs. NCBI nr
Match: gi|659103276|ref|XP_008452556.1| (PREDICTED: protein DCL, chloroplastic [Cucumis melo])

HSP 1 Score: 445.7 bits (1145), Expect = 5.0e-122
Identity = 212/225 (94.22%), Postives = 218/225 (96.89%), Query Frame = 1 

Query: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRIRSHQ 60
           MAMASIL   PFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEG RIRSHQ
Sbjct: 1   MAMASILTLLPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGSRIRSHQ 60

Query: 61  EYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPV P AKDLAGPSEDDDSSEESGN++EEEV+WVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDLAGPSEDDDSSEESGNDEEEEVDWVDWEDKILEDTVPLVGFVR 120

Query: 121 MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180
           MVLHTGKYE+GDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYESGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 181 VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRSSRR 226
           VRKDGE+VDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRR+ RR
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNLRR 225

[Close]
BLAST of Cucsa.352210 vs. NCBI nr
Match: gi|590592205|ref|XP_007017215.1| (Uncharacterized protein isoform 1 [Theobroma cacao])

HSP 1 Score: 298.9 bits (764), Expect = 7.5e-78
Identity = 144/220 (65.45%), Postives = 175/220 (79.55%), Query Frame = 1 

Query: 3   MASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRIRSHQEY 62
           MAS+LKPPP+   + ++ +  +SSP+IL  P+    S    + AL+TG +G RI S + Y
Sbjct: 1   MASVLKPPPYFHRNCISISS-SSSPVILSSPSQRTTSLQVRSCALRTGSDGGRIGSQESY 60

Query: 63  SSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVRMV 122
            +D+LRKP   + KD  G SE ++ SE       +  +W+DWED+ILEDTVPLVGFVRM+
Sbjct: 61  GADMLRKPSILTPKDSGGTSEQEEGSEGK----RKRGKWIDWEDRILEDTVPLVGFVRMI 120

Query: 123 LHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFIVR 182
           +H+GKYE+GDRL PEHEKTIL+RLLPYHPE EKKIGCG+DYITVGYHPDFE SRCLFIVR
Sbjct: 121 IHSGKYESGDRLSPEHEKTILDRLLPYHPECEKKIGCGIDYITVGYHPDFEGSRCLFIVR 180

Query: 183 KDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRS 223
           KDGE++DFSYWKCIKGLIRKNYPLYA+SFILRHFRRRRRS
Sbjct: 181 KDGELIDFSYWKCIKGLIRKNYPLYADSFILRHFRRRRRS 215

[Close]
BLAST of Cucsa.352210 vs. NCBI nr
Match: gi|255538290|ref|XP_002510210.1| (PREDICTED: protein DCL, chloroplastic [Ricinus communis])

HSP 1 Score: 295.8 bits (756), Expect = 6.3e-77
Identity = 149/224 (66.52%), Postives = 177/224 (79.02%), Query Frame = 1 

Query: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTH-PINSFHPSTRALKTGPEGIRIRSH 60
           M MAS+ KPPP    H  NP     SP+IL FP H    SF+    ALKTG +G      
Sbjct: 1   MTMASLSKPPPCLHGHYSNPISLYFSPVILSFPFHRTTTSFNSRIFALKTGSDG------ 60

Query: 61  QEYSSDLLRKPVGPSAKDLAGPSEDD-DSSEESGNEDEEEVEWVDWEDKILEDTVPLVGF 120
               SDLLRKP+ PS K+L+G S+D+ DS+ +  N+D+E+ E VDWED+ILEDTVPLVGF
Sbjct: 61  ----SDLLRKPIVPSEKELSGISDDEEDSNRKRDNKDKEDDELVDWEDQILEDTVPLVGF 120

Query: 121 VRMVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCL 180
           VRM+LH+GKYENGDRL PEHE+TI+ERLLP+HPE EKKIG G+DYITVG+H +FE+SRCL
Sbjct: 121 VRMILHSGKYENGDRLSPEHERTIVERLLPFHPECEKKIGPGIDYITVGHHTEFENSRCL 180

Query: 181 FIVRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRS 223
           FIVRKDG++VDFSYWKCIKGLIRKNYPLYA+SFILRHFRRRR+S
Sbjct: 181 FIVRKDGKLVDFSYWKCIKGLIRKNYPLYADSFILRHFRRRRQS 214

[Close]
BLAST of Cucsa.352210 vs. NCBI nr
Match: gi|823238509|ref|XP_012451906.1| (PREDICTED: protein DCL, chloroplastic-like isoform X1 [Gossypium raimondii])

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-76
Identity = 146/221 (66.06%), Postives = 167/221 (75.57%), Query Frame = 1 

Query: 3   MASILKPPPFPPFHSLN-PNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRIRSHQE 62
           MASIL PPP P FH  + P   +SSP+IL +P     S     +AL+T  +G +I S + 
Sbjct: 1   MASILNPPPPPYFHRTSMPTSVSSSPVILSYPFLKTTSLQVRFKALRTWSDGGKIGSQEA 60

Query: 63  YSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVRM 122
           Y +D LRKP     KD  G  E+++ SE   N  E    W DWED+ILEDTVPLVGFVRM
Sbjct: 61  YGADFLRKPSTVPKKDSDGILEEEEGSEGKRNRGE----WTDWEDRILEDTVPLVGFVRM 120

Query: 123 VLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFIV 182
           ++H+GKY  GDRL PEHE+TILERLLPYHPE EKKIGCG+DYITVGYHPDF  SRCLFIV
Sbjct: 121 IIHSGKYGAGDRLSPEHERTILERLLPYHPEFEKKIGCGIDYITVGYHPDFVGSRCLFIV 180

Query: 183 RKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRS 223
           RKDGE+VDFSYWKCIKGLIRKNYPLYA+SFILRHFRRRRRS
Sbjct: 181 RKDGELVDFSYWKCIKGLIRKNYPLYADSFILRHFRRRRRS 217

The following BLAST results are available for this feature:
BLAST of Cucsa.352210 vs. Swiss-Prot
Match NameE-valueIdentityDescription
DCL_SOLLC7.8e-6768.89Protein DCL, chloroplastic OS=Solanum lycopersicum GN=DCL PE=2 SV=1[more]
NRPE1_ARATH2.2e-2139.67DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana GN=NRPE1 PE=1 SV... [more]
BLAST of Cucsa.352210 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L2D0_CUCSA4.5e-13099.11Uncharacterized protein OS=Cucumis sativus GN=Csa_4G639820 PE=4 SV=1[more]
A0A061FBM3_THECC5.2e-7865.45Uncharacterized protein isoform 1 OS=Theobroma cacao GN=TCM_033826 PE=4 SV=1[more]
B9R7B7_RICCO4.4e-7766.52DCL protein, chloroplast, putative OS=Ricinus communis GN=RCOM_1590270 PE=4 SV=1[more]
A0A0D2R0L6_GOSRA7.5e-7766.06Uncharacterized protein OS=Gossypium raimondii GN=B456_010G020200 PE=4 SV=1[more]
A0A0B0NSM0_GOSAR9.8e-7766.06Protein DCL, chloroplastic OS=Gossypium arboreum GN=F383_03623 PE=4 SV=1[more]
BLAST of Cucsa.352210 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G45230.12.9e-7260.89 Protein of unknown function (DUF3223)[more]
AT3G46630.12.3e-3248.80 Protein of unknown function (DUF3223)[more]
AT2G40030.11.2e-2239.67 nuclear RNA polymerase D1B[more]
AT5G62440.16.2e-0630.23 Protein of unknown function (DUF3223)[more]
BLAST of Cucsa.352210 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449447003|ref|XP_004141259.1|6.5e-13099.11PREDICTED: protein DCL, chloroplastic [Cucumis sativus][more]
gi|659103276|ref|XP_008452556.1|5.0e-12294.22PREDICTED: protein DCL, chloroplastic [Cucumis melo][more]
gi|590592205|ref|XP_007017215.1|7.5e-7865.45Uncharacterized protein isoform 1 [Theobroma cacao][more]
gi|255538290|ref|XP_002510210.1|6.3e-7766.52PREDICTED: protein DCL, chloroplastic [Ricinus communis][more]
gi|823238509|ref|XP_012451906.1|1.1e-7666.06PREDICTED: protein DCL, chloroplastic-like isoform X1 [Gossypium raimondii][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR021602Protein of unknown function DUF3223
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009308 amine metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005507 copper ion binding
molecular_function GO:0048038 quinone binding
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.352210.1Cucsa.352210.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021602Protein of unknown function DUF3223PFAMPF11523DUF3223coord: 121..196
score: 2.8
NoneNo IPR availableGENE3DG3DSA:3.10.450.40coord: 100..204
score: 1.9
NoneNo IPR availablePANTHERPTHR33415FAMILY NOT NAMEDcoord: 1..221
score: 7.1E
NoneNo IPR availablePANTHERPTHR33415:SF2SUBFAMILY NOT NAMEDcoord: 1..221
score: 7.1E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.352210Cla020637Watermelon (97103) v1cgywmB634
Cucsa.352210Cla020670Watermelon (97103) v1cgywmB631
Cucsa.352210Csa4G639820Cucumber (Chinese Long) v2cgycuB494
Cucsa.352210MELO3C016755Melon (DHL92) v3.5.1cgymeB570
Cucsa.352210ClCG05G019910Watermelon (Charleston Gray)cgywcgB599
Cucsa.352210ClCG05G020220Watermelon (Charleston Gray)cgywcgB602
Cucsa.352210CSPI04G24100Wild cucumber (PI 183967)cgycpiB521
Cucsa.352210CmaCh09G002290Cucurbita maxima (Rimu)cgycmaB0960
Cucsa.352210CmaCh01G018140Cucurbita maxima (Rimu)cgycmaB0964
Cucsa.352210CmaCh01G018370Cucurbita maxima (Rimu)cgycmaB0965
Cucsa.352210CmaCh09G002590Cucurbita maxima (Rimu)cgycmaB0961
Cucsa.352210CmoCh01G018700Cucurbita moschata (Rifu)cgycmoB0966
Cucsa.352210CmoCh09G002520Cucurbita moschata (Rifu)cgycmoB0962
Cucsa.352210CmoCh09G002220Cucurbita moschata (Rifu)cgycmoB0961
Cucsa.352210CmoCh01G018960Cucurbita moschata (Rifu)cgycmoB0967
Cucsa.352210Lsi04G015610Bottle gourd (USVL1VR-Ls)cgylsiB570
Cucsa.352210Cp4.1LG02g06820Cucurbita pepo (Zucchini)cgycpeB0921
Cucsa.352210Cp4.1LG02g06870Cucurbita pepo (Zucchini)cgycpeB0922
Cucsa.352210Cp4.1LG06g01680Cucurbita pepo (Zucchini)cgycpeB0923
Cucsa.352210Cp4.1LG06g01650Cucurbita pepo (Zucchini)cgycpeB0924
Cucsa.352210MELO3C016755.2Melon (DHL92) v3.6.1cgymedB571
Cucsa.352210CsaV3_4G034350Cucumber (Chinese Long) v3cgycucB536
Cucsa.352210Cla97C05G101550Watermelon (97103) v2cgywmbB602
Cucsa.352210Cla97C05G101930Watermelon (97103) v2cgywmbB605
Cucsa.352210Bhi09G002729Wax gourdcgywgoB706
Cucsa.352210Bhi09G002697Wax gourdcgywgoB705
Cucsa.352210Carg27331Silver-seed gourdcarcgyB0961
Cucsa.352210Carg26300Silver-seed gourdcarcgyB0902
Cucsa.352210Carg10621Silver-seed gourdcarcgyB0878
Cucsa.352210Carg10606Silver-seed gourdcarcgyB0877
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.352210Melon (DHL92) v3.6.1cgymedB568
Cucsa.352210Melon (DHL92) v3.6.1cgymedB569
Cucsa.352210Silver-seed gourdcarcgyB0224
Cucsa.352210Cucumber (Chinese Long) v3cgycucB535
Cucsa.352210Cucumber (Chinese Long) v3cgycucB540
Cucsa.352210Watermelon (97103) v2cgywmbB601
Cucsa.352210Watermelon (97103) v2cgywmbB606
Cucsa.352210Wax gourdcgywgoB702
Cucsa.352210Cucumber (Gy14) v1cgycgyB118
Cucsa.352210Cucumber (Gy14) v1cgycgyB148
Cucsa.352210Cucurbita maxima (Rimu)cgycmaB0967
Cucsa.352210Cucurbita moschata (Rifu)cgycmoB0969
Cucsa.352210Cucurbita moschata (Rifu)cgycmoB0963
Cucsa.352210Wild cucumber (PI 183967)cgycpiB520
Cucsa.352210Wild cucumber (PI 183967)cgycpiB525
Cucsa.352210Cucumber (Chinese Long) v2cgycuB493
Cucsa.352210Melon (DHL92) v3.5.1cgymeB567
Cucsa.352210Melon (DHL92) v3.5.1cgymeB568
Cucsa.352210Watermelon (Charleston Gray)cgywcgB603
Cucsa.352210Watermelon (97103) v1cgywmB630
Cucsa.352210Cucurbita pepo (Zucchini)cgycpeB0919
Cucsa.352210Bottle gourd (USVL1VR-Ls)cgylsiB571