Carg27331 (gene) Silver-seed gourd

Overview
NameCarg27331
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionDNA-directed RNA polymerase subunit
LocationCucurbita_argyrosperma_scaffold_549 : 32451 .. 35896 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGCGGATTGAAGAGAAATATTGTAGAATCTCCAAAACCATGAGATTGATGATAATGTGAGTCACCATTAACAGTTATTTTTCCTCCGTTTTCTAAGGGAATTGGGTGAAAAACCTCAGTAGTTGCAAATTTGAGCTCCTTGATTCAATTGAACAAGGGCATGGTCCTGGCTATGGCCTCCATTTTGAAGCCACCGCCTCTTGTGCCCCTTCACTCCCTAAATCCAAACCTCCTCAACGCTTCACCAGTGATCGTATGTTTTCGTACGCACCCCATGACGTCGTTTCACCCATCCATTCGTGCCCTAAAAACTGGCCCTGAAGGCAGTAGGATTCGAAGCCACGAAGAATATAGTTCCGATTTGCTGCGAAAACCGGTTGTGCCGCCGGCGATGGACCTGGCTACACCATCGGAGGATTACAGCAGCAGTGAGGAGAGTGGAAACGGAGACGAGGAGGAAGAGGAATGGGTTGACTGGGAGGACAGGATTCTGGAGGAGACTGTTCCTCTAGTTGGCTTTGTTAGGATGGTTCTTCACTCTGGAAAGTAAGCAACCATTCGTTTGTTTATCGGCTTTTTAACTGAATTTGAGCTTCCAGGCAACTAGAAACCATGCAACCATGTTATTAAGTGAATTTTGAGATTGTTATTGCTCAAATGAAAGCGTGTGCTCAAAGATGAACGGAACTAAATAATTTGAGATTGAAATTTTAGAACTTGTACGGAACGTTCATCATTTCCATAGCAAGCAAGAACAACGATGACGAGTTCTCTGTCAATTAATCTCTTCAAAGTTCATTGATCTTTTATTCCATTTGATCAAATTTAATTATAACTTGACGCACTTAAAAGACTTAGAATCTAATAAATTGAAGAGAAAAAGGCTCTTGCAACCGTACACACTCAACCTATGGTCCTTAGCCCATGGATAAGTCAATTGTTAACGTATCTTATTTCTGTTTTCTCATATTTCAACGATCAAGTGTTCGTAACATGTGTATTGTTATCTTTCAGTGAAAGTTTCAAGTTGTTATCATTTAGTATCTCCTTACTGACAAAATTTGAACGTTTTATAGCTGTTTTCTTCCCTTGGATGGATGAGATATCTTGACTGTTTGCTTTGATCTTGTTAATTCACCGATGGTGCACCTATTATCTGAAAGACAATATGTTGTGTCTTGAATTGACTAGCATGGTGTGGTAGAGTTTAATCTCCATTCATTAATCATCTATAAGCCAACATGCGCAGATATGAAAGTGGGGATAGGTTGACCCCAGAGCACGAGAAAACAATTCTTGATCGGTTGCTTTCATATCATCCCGAGTGTGAGAAGAAGATTGGATGTGGGATTGATTATATCACGGTATATCTCTTTCCATATTTCTACATGCCTCGTGTTTTTATCTTATTTTTAGAATTTGCATTTATATTAAATAAAATTACTAATGAGATACTCTAGCCTCTTTTCTGTTTCCTTGGATGAATGAAAGCTTTTGTCATGAACCTGGAAAAGAAATTAAATGTTCACAATTAAGTTTTTAATTGCTCCTGATGAAACATCTTGATTATCGCTATTGATGAGATTGACTTCAACAAGTCATGATTCATCCCTGAAACATTTGACTAGCTTAGCTTGTAATCCTAACATATTTGTCTATAATGTTTGACTTGAAAAGATATAAATTGTTACGGAGCAGAACATGAATTAGAAGGGAAAACTTACTTTTCCTAATACACGTGATCTATATCGACTGTGTTTTTTTTTTTTTTTTTTTTTTCCTTTTGTAAAAATACTTTGCCTGGGATTTATATGAAGACACTTCAAAGTAATTCTCAATGTACACAGTGAGAAATGTGGGCTTTGATAAGTGGCAACGGTTTGATATTTGTTGAAACATTGTATTTTCACTAGATGGTGTATACGTGATGCTGCCAAAAGGGTTATACCTCGACTTGAACTCCAATATCTTGCCATTAGAAAAGCGATTTTATGGGATGCCATTTTCATTTTTGAACCATTTTCTTTGAAATCATATTGAGCACTGGATACAAGCTCTGTCTCTGTCAACTTAATCATATTGTTGTGGTCGGCAGATTGGATATCATCCGAATTTTGAAAGGTCAAGATGTTTATTCATAGTCAGAAAAGATGGAGAGTTGGTTGATTTTTCATATTGGAAGTGCATGGAGGGTCTGATCAGAAAGAACTATCCTCTTCATGCAGAAAGCTTTATTCTTCGGCATTTTCAGCGACGTAGACGCAATTTTTTTCGGTAACGAATGCAAGATCTGTTAATCATACTATTGTACCATCCAATTTCAGGAATTCTTTGAATTTCGAAGCTTGAAATTAACCTACTACGCTTGTGTGGTTCTCGTGTTTAATGATTTGAAATGCGCGAATCAAGAAGAATATAGGCTGAATGTAGACTCTTGCTCGAGGAAGGTGCTAGCAAAATTCAATATTCGAGCCTAACAGTTACCTCCAGTCCAGTTCTTGCTTAGTCTCTAGTGGTTAACTGGTTAGCTACATTCCCTCGCTCTCTCATTAGCAGGAATATATGCTCAAGATCCTTGAACCTTGTAGCATTTTGTAGTTTGGCCGGTATGTATTAAAACTCTATTGAATTGGCATCAAAAACCCTTAAATTCTGTATGGAGTTCATATATTATGTTATTGTGAGTAAGAAAAAATACAATGGCGCCCAAATGGGCATATTCTTGTATTCGACCATGGCTAAAGCTAAAGTACTGCAACTGAGCTGCTACTCTGCTCGACTTCAGCGGAGTAGCTTTCGTCATGTAGGGAAATTCTCAGCCTTAACTTTGCATCTAAATTTTGTGTTTTGATCTTTCCATCCTTCATGGCACTATGTGTACGTCTTACAACTTGTGGATGAAAATTATGAAGGGTATTAAGTTTTTAACCTCCATGGTTCTCTTGAATTTTAAAAAGTGAATATTGTAAAATAAACAATAATATTAAAAATTCTAGCAAAACTCGGGGAAGTCAATCAACAAACATGATTCTCTCGGTAGTCAATCGATACAATTGAAACTCATAACCTTATGAGTTTTGACATGTAAAGTTACGCCTCAGTGCTATATAGTAATAATTCTCTCAATTACCCAACTCGATTACAAAGAAAAGGGATTTAAGGAAAACGAACTCTCATTATATACACAAATGCACGAGAACAAAGTGATCAGTTTGGCTTAACATTCAAGCTTGTATTTGGTCAAGGAGGTTGCGCATCATCTACCTATAGCTGAGAATCGGGTTCAAGCATGTAGCAATCCATGGCCACTACATCAATCAATATTTCAATACTGGAGAAATTGGCTAAGAAGGAAATTATCACATTCTTCATATTTTCCAGGCAGGCAGATTTTCTAGAACTGTTCCACCATCAGCAGCAGCAGCAGGAGGAGGTGCTGAAGTAA

mRNA sequence

AATGCGGATTGAAGAGAAATATTGTAGAATCTCCAAAACCATGAGATTGATGATAATGTGAGTCACCATTAACAGTTATTTTTCCTCCGTTTTCTAAGGGAATTGGGTGAAAAACCTCAGTAGTTGCAAATTTGAGCTCCTTGATTCAATTGAACAAGGGCATGGTCCTGGCTATGGCCTCCATTTTGAAGCCACCGCCTCTTGTGCCCCTTCACTCCCTAAATCCAAACCTCCTCAACGCTTCACCAGTGATCGTATGTTTTCGTACGCACCCCATGACGTCGTTTCACCCATCCATTCGTGCCCTAAAAACTGGCCCTGAAGGCAGTAGGATTCGAAGCCACGAAGAATATAGTTCCGATTTGCTGCGAAAACCGGTTGTGCCGCCGGCGATGGACCTGGCTACACCATCGGAGGATTACAGCAGCAGTGAGGAGAGTGGAAACGGAGACGAGGAGGAAGAGGAATGGGTTGACTGGGAGGACAGGATTCTGGAGGAGACTGTTCCTCTAGTTGGCTTTGTTAGGATGGTTCTTCACTCTGGAAAATATGAAAGTGGGGATAGGTTGACCCCAGAGCACGAGAAAACAATTCTTGATCGGTTGCTTTCATATCATCCCGAGTGTGAGAAGAAGATTGGATGTGGGATTGATTATATCACGATTGGATATCATCCGAATTTTGAAAGGTCAAGATGTTTATTCATAGTCAGAAAAGATGGAGAGTTGGTTGATTTTTCATATTGGAAGTGCATGGAGGGTCTGATCAGAAAGAACTATCCTCTTCATGCAGAAAGCTTTATTCTTCGGCATTTTCAGCGACGTAGACGCAATTTTTTTCGGCAGGCAGATTTTCTAGAACTGTTCCACCATCAGCAGCAGCAGCAGGAGGAGGTGCTGAAGTAA

Coding sequence (CDS)

ATGGTCCTGGCTATGGCCTCCATTTTGAAGCCACCGCCTCTTGTGCCCCTTCACTCCCTAAATCCAAACCTCCTCAACGCTTCACCAGTGATCGTATGTTTTCGTACGCACCCCATGACGTCGTTTCACCCATCCATTCGTGCCCTAAAAACTGGCCCTGAAGGCAGTAGGATTCGAAGCCACGAAGAATATAGTTCCGATTTGCTGCGAAAACCGGTTGTGCCGCCGGCGATGGACCTGGCTACACCATCGGAGGATTACAGCAGCAGTGAGGAGAGTGGAAACGGAGACGAGGAGGAAGAGGAATGGGTTGACTGGGAGGACAGGATTCTGGAGGAGACTGTTCCTCTAGTTGGCTTTGTTAGGATGGTTCTTCACTCTGGAAAATATGAAAGTGGGGATAGGTTGACCCCAGAGCACGAGAAAACAATTCTTGATCGGTTGCTTTCATATCATCCCGAGTGTGAGAAGAAGATTGGATGTGGGATTGATTATATCACGATTGGATATCATCCGAATTTTGAAAGGTCAAGATGTTTATTCATAGTCAGAAAAGATGGAGAGTTGGTTGATTTTTCATATTGGAAGTGCATGGAGGGTCTGATCAGAAAGAACTATCCTCTTCATGCAGAAAGCTTTATTCTTCGGCATTTTCAGCGACGTAGACGCAATTTTTTTCGGCAGGCAGATTTTCTAGAACTGTTCCACCATCAGCAGCAGCAGCAGGAGGAGGTGCTGAAGTAA

Protein sequence

MVLAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRSHEEYSSDLLRKPVVPPAMDLATPSEDYSSSEESGNGDEEEEEWVDWEDRILEETVPLVGFVRMVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCLFIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQRRRRNFFRQADFLELFHHQQQQQEEVLK
Homology
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BLAST of Carg27331 vs. NCBI nr
Match: XP_022937348.1 (protein DCL homolog, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 431.0 bits (1107), Expect = 2.7e-117
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0 

Query: 1   MVLAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRS 60
           MVLAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRS
Sbjct: 1   MVLAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRS 60

Query: 61  HEEYSSDLLRKPVVPPAMDLATPSEDYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGF 120
           HEEYSSDLLRKPVVPPAMDLATPSEDYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGF
Sbjct: 61  HEEYSSDLLRKPVVPPAMDLATPSEDYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGF 120

Query: 121 VRMVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCL 180
           VRMVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCL
Sbjct: 121 VRMVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCL 180

Query: 181 FIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
           FIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR
Sbjct: 181 FIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 220

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BLAST of Carg27331 vs. NCBI nr
Match: XP_022975974.1 (protein DCL homolog, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 423.3 bits (1087), Expect = 5.6e-115
Identity = 210/220 (95.45%), Postives = 212/220 (96.36%), Query Frame = 0 

Query: 1   MVLAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRS 60
           MVLAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRS
Sbjct: 1   MVLAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRS 60

Query: 61  HEEYSSDLLRKPVVPPAMDLATPSEDYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGF 120
           HEEYSSDLLRKPVVPPAMDLATPSEDY+     XXXXXXXX WVDWEDRILEETVPLVGF
Sbjct: 61  HEEYSSDLLRKPVVPPAMDLATPSEDYNSSEESXXXXXXXXEWVDWEDRILEETVPLVGF 120

Query: 121 VRMVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCL 180
           VRMVLHSGKY SGDRL+PEHEKTILDRLL YHPECEKKIGCGIDYITIGYHPNFERSRCL
Sbjct: 121 VRMVLHSGKYGSGDRLSPEHEKTILDRLLLYHPECEKKIGCGIDYITIGYHPNFERSRCL 180

Query: 181 FIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
           FIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR
Sbjct: 181 FIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 220

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BLAST of Carg27331 vs. NCBI nr
Match: XP_023536599.1 (protein DCL homolog, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 420.2 bits (1079), Expect = 4.7e-114
Identity = 211/220 (95.91%), Postives = 213/220 (96.82%), Query Frame = 0 

Query: 1   MVLAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRS 60
           MVLAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFH SIRALKTGPEGSRIRS
Sbjct: 1   MVLAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHQSIRALKTGPEGSRIRS 60

Query: 61  HEEYSSDLLRKPVVPPAMDLATPSEDYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGF 120
           HEEYSSDLLRKPVVPPA+DLAT SEDY+   XXXXXXXXXX WVDWEDRILEETVPLVGF
Sbjct: 61  HEEYSSDLLRKPVVPPAIDLATASEDYNSSEXXXXXXXXXXEWVDWEDRILEETVPLVGF 120

Query: 121 VRMVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCL 180
           VRMVLHSGKYESGDRLTPEHEKTILDRLL YHPECEKKIGCGIDYITIGYHPNFERSRCL
Sbjct: 121 VRMVLHSGKYESGDRLTPEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPNFERSRCL 180

Query: 181 FIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
           FIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR
Sbjct: 181 FIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 220

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BLAST of Carg27331 vs. NCBI nr
Match: XP_004141259.1 (PREDICTED: protein DCL, chloroplastic [Cucumis sativus] >KGN55194.1 hypothetical protein Csa_4G639820 [Cucumis sativus])

HSP 1 Score: 357.5 bits (916), Expect = 3.8e-95
Identity = 176/218 (80.73%), Postives = 196/218 (89.91%), Query Frame = 0 

Query: 3   LAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRSHE 62
           +AMASILKPPP  P HSLNPN  N+SP+I+CF THP+ SFHPS RALKTGPEG R+RSH+
Sbjct: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRLRSHQ 60

Query: 63  EYSSDLLRKPVVPPAMDLATPSEDYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGFVR 122
           EYSSDLLRKPV P A DLA PSE   XXXXXXXXXXXX  WVDWED+ILE+TVPLVGFVR
Sbjct: 61  EYSSDLLRKPVGPSAKDLAGPSEXXXXXXXXXXXXXXXVEWVDWEDKILEDTVPLVGFVR 120

Query: 123 MVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCLFI 182
           MVLH+GKYE+GDRL PEHEKTIL+RLL YHPE EKKIGCG+DYIT+GYHP+FE SRCLFI
Sbjct: 121 MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 183 VRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
           VRKDGE+VDFSYWKC++GLIRKNYPL+AESFILRHF+R
Sbjct: 181 VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRR 218

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BLAST of Carg27331 vs. NCBI nr
Match: XP_008452556.1 (PREDICTED: protein DCL, chloroplastic [Cucumis melo])

HSP 1 Score: 354.0 bits (907), Expect = 4.2e-94
Identity = 177/218 (81.19%), Postives = 194/218 (88.99%), Query Frame = 0 

Query: 3   LAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRSHE 62
           +AMASIL   P  P HSLNPN  N+SP+I+CF THP+ SFHPS RALKTGPEGSRIRSH+
Sbjct: 1   MAMASILTLLPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGSRIRSHQ 60

Query: 63  EYSSDLLRKPVVPPAMDLATPSEDYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGFVR 122
           EYSSDLLRKPVVPPA DLA P     XXXXXXXXXXXX  WVDWED+ILE+TVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDLAGPXXXXXXXXXXXXXXXXXVDWVDWEDKILEDTVPLVGFVR 120

Query: 123 MVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCLFI 182
           MVLH+GKYESGDRL PEHEKTIL+RLL YHPE EKKIGCG+DYIT+GYHP+FE SRCLFI
Sbjct: 121 MVLHTGKYESGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 183 VRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
           VRKDGELVDFSYWKC++GLIRKNYPL+AESFILRHF+R
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLYAESFILRHFRR 218

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BLAST of Carg27331 vs. TAIR10
Match: AT1G45230.1 (Protein of unknown function (DUF3223))

HSP 1 Score: 239.2 bits (609), Expect = 2.7e-63
Identity = 120/195 (61.54%), Postives = 153/195 (78.46%), Query Frame = 0 

Query: 26  NASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRSHEEYSSDLLRKPVVPPAMDLATPSE 85
           ++SP+ + F     TS  P +RAL+T  +G++I + E Y S+LLR+P +           
Sbjct: 27  SSSPLCLYFPRGDSTSLRPRVRALRTESDGAKIGNSESYGSELLRRPRI-------AXXX 86

Query: 86  DYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGFVRMVLHSGKYESGDRLTPEHEKTIL 145
              XXXXXXXXXXX   +VDWED+ILE TVPLVGFVRM+LHSGKY + DRL+PEHE+TI+
Sbjct: 87  XXXXXXXXXXXXXXGDEFVDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTII 146

Query: 146 DRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCLFIVRKDGELVDFSYWKCMEGLIRKN 205
           + LL YHPECEKKIGCGIDYI +G+HP+FE SRC+FIVRKDGE+VDFSYWKC++GLI+K 
Sbjct: 147 EMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKK 206

Query: 206 YPLHAESFILRHFQR 221
           YPL+A+SFILRHF++
Sbjct: 207 YPLYADSFILRHFRK 214

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BLAST of Carg27331 vs. TAIR10
Match: AT3G46630.1 (Protein of unknown function (DUF3223))

HSP 1 Score: 133.7 bits (335), Expect = 1.6e-31
Identity = 56/118 (47.46%), Postives = 76/118 (64.41%), Query Frame = 0 

Query: 103 WVDWEDRILEETVPLVGFVRMVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCG 162
           W + E  IL +  P+    + +LHS +Y  G+RL  E EK ++++LL YHP  + KIGCG
Sbjct: 87  WKNLEAEILRDIEPISLLAKEILHSDRYLDGERLDFEDEKIVMEKLLPYHPYSKDKIGCG 146

Query: 163 IDYITIGYHPNFERSRCLFIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
           +D+I +  HP F  SRCLF+VR DG  +DFSY KC+   +R  YP HAE FI  HF+R
Sbjct: 147 LDFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYVRDKYPSHAERFIREHFKR 204

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BLAST of Carg27331 vs. TAIR10
Match: AT2G40030.1 (nuclear RNA polymerase D1B)

HSP 1 Score: 102.8 bits (255), Expect = 3.0e-22
Identity = 41/114 (35.96%), Postives = 69/114 (60.53%), Query Frame = 0 

Query: 107  EDRILEETVPLVGFVRMVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYI 166
            E  +L +  P++  +R ++H   Y  GD ++ + +  +L+++L++HP+ E K+G G+D+I
Sbjct: 1736 EQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDFI 1795

Query: 167  TIGYHPNFERSRCLFIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
            T+  H  F  SRC F+V  DG   DFSY K +   + K YP  AE FI ++F +
Sbjct: 1796 TVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTK 1849

[Close]
BLAST of Carg27331 vs. TAIR10
Match: AT5G62440.1 (Protein of unknown function (DUF3223))

HSP 1 Score: 53.9 bits (128), Expect = 1.6e-07
Identity = 25/59 (42.37%), Postives = 37/59 (62.71%), Query Frame = 0 

Query: 144 ILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCLFIVRKDGELVDFSYWKCMEGLI 203
           +LD +   H E EKKIG GI    +  HP + +SRC F+VR+D    DFS+ KC++ ++
Sbjct: 105 LLDLIKKGHSEPEKKIGGGIKTFQVRTHPMW-KSRCFFLVREDDTADDFSFRKCVDQIL 162

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BLAST of Carg27331 vs. Swiss-Prot
Match: sp|Q42463|DCL_SOLLC (Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 9.9e-63
Identity = 111/176 (63.07%), Postives = 137/176 (77.84%), Query Frame = 0 

Query: 48  ALKTGPEGSRIRSHEEYSSDLLRKPVVPPAMDLATPSEDY---SXXXXXXXXXXXXXXWV 107
           A+KTG EG  IRS    +++LLRKPV+   ++  + SE+                   WV
Sbjct: 47  AVKTGSEGGGIRSD---NAELLRKPVISTELETTSESEELVKEESDDEVGKKSGDGEGWV 106

Query: 108 DWEDRILEETVPLVGFVRMVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGID 167
           DWED+ILE+TVPLVGFVRM+LHSGKY  GDRL+P+H++TIL RLL YHPEC+KKIG G+D
Sbjct: 107 DWEDQILEDTVPLVGFVRMILHSGKYAIGDRLSPDHQRTILQRLLPYHPECDKKIGPGVD 166

Query: 168 YITIGYHPNFERSRCLFIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
           YIT+GYHP+FE SRCLFIVRKDGE VDFSYWKC++GLIRKNYPL+A+SFILRHF++
Sbjct: 167 YITVGYHPDFENSRCLFIVRKDGETVDFSYWKCIKGLIRKNYPLYADSFILRHFRK 219

[Close]
BLAST of Carg27331 vs. Swiss-Prot
Match: sp|Q9C642|DCL_ARATH (Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 4.9e-62
Identity = 120/195 (61.54%), Postives = 153/195 (78.46%), Query Frame = 0 

Query: 26  NASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRSHEEYSSDLLRKPVVPPAMDLATPSE 85
           ++SP+ + F     TS  P +RAL+T  +G++I + E Y S+LLR+P +           
Sbjct: 27  SSSPLCLYFPRGDSTSLRPRVRALRTESDGAKIGNSESYGSELLRRPRI-------AXXX 86

Query: 86  DYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGFVRMVLHSGKYESGDRLTPEHEKTIL 145
              XXXXXXXXXXX   +VDWED+ILE TVPLVGFVRM+LHSGKY + DRL+PEHE+TI+
Sbjct: 87  XXXXXXXXXXXXXXGDEFVDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTII 146

Query: 146 DRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCLFIVRKDGELVDFSYWKCMEGLIRKN 205
           + LL YHPECEKKIGCGIDYI +G+HP+FE SRC+FIVRKDGE+VDFSYWKC++GLI+K 
Sbjct: 147 EMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKK 206

Query: 206 YPLHAESFILRHFQR 221
           YPL+A+SFILRHF++
Sbjct: 207 YPLYADSFILRHFRK 214

[Close]
BLAST of Carg27331 vs. Swiss-Prot
Match: sp|Q5D869|NRPE1_ARATH (DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 5.5e-21
Identity = 41/114 (35.96%), Postives = 69/114 (60.53%), Query Frame = 0 

Query: 107  EDRILEETVPLVGFVRMVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYI 166
            E  +L +  P++  +R ++H   Y  GD ++ + +  +L+++L++HP+ E K+G G+D+I
Sbjct: 1736 EQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDFI 1795

Query: 167  TIGYHPNFERSRCLFIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
            T+  H  F  SRC F+V  DG   DFSY K +   + K YP  AE FI ++F +
Sbjct: 1796 TVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTK 1849

[Close]
BLAST of Carg27331 vs. Swiss-Prot
Match: sp|Q8L557|EM514_ARATH (Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.9e-06
Identity = 25/59 (42.37%), Postives = 37/59 (62.71%), Query Frame = 0 

Query: 144 ILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCLFIVRKDGELVDFSYWKCMEGLI 203
           +LD +   H E EKKIG GI    +  HP + +SRC F+VR+D    DFS+ KC++ ++
Sbjct: 105 LLDLIKKGHSEPEKKIGGGIKTFQVRTHPMW-KSRCFFLVREDDTADDFSFRKCVDQIL 162

[Close]
BLAST of Carg27331 vs. TrEMBL
Match: tr|A0A0A0L2D0|A0A0A0L2D0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639820 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 2.5e-95
Identity = 176/218 (80.73%), Postives = 196/218 (89.91%), Query Frame = 0 

Query: 3   LAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRSHE 62
           +AMASILKPPP  P HSLNPN  N+SP+I+CF THP+ SFHPS RALKTGPEG R+RSH+
Sbjct: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRLRSHQ 60

Query: 63  EYSSDLLRKPVVPPAMDLATPSEDYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGFVR 122
           EYSSDLLRKPV P A DLA PSE   XXXXXXXXXXXX  WVDWED+ILE+TVPLVGFVR
Sbjct: 61  EYSSDLLRKPVGPSAKDLAGPSEXXXXXXXXXXXXXXXVEWVDWEDKILEDTVPLVGFVR 120

Query: 123 MVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCLFI 182
           MVLH+GKYE+GDRL PEHEKTIL+RLL YHPE EKKIGCG+DYIT+GYHP+FE SRCLFI
Sbjct: 121 MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 183 VRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
           VRKDGE+VDFSYWKC++GLIRKNYPL+AESFILRHF+R
Sbjct: 181 VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRR 218

[Close]
BLAST of Carg27331 vs. TrEMBL
Match: tr|A0A1S3BVA4|A0A1S3BVA4_CUCME (protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493543 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 2.8e-94
Identity = 177/218 (81.19%), Postives = 194/218 (88.99%), Query Frame = 0 

Query: 3   LAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRSHE 62
           +AMASIL   P  P HSLNPN  N+SP+I+CF THP+ SFHPS RALKTGPEGSRIRSH+
Sbjct: 1   MAMASILTLLPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGSRIRSHQ 60

Query: 63  EYSSDLLRKPVVPPAMDLATPSEDYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGFVR 122
           EYSSDLLRKPVVPPA DLA P     XXXXXXXXXXXX  WVDWED+ILE+TVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDLAGPXXXXXXXXXXXXXXXXXVDWVDWEDKILEDTVPLVGFVR 120

Query: 123 MVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCLFI 182
           MVLH+GKYESGDRL PEHEKTIL+RLL YHPE EKKIGCG+DYIT+GYHP+FE SRCLFI
Sbjct: 121 MVLHTGKYESGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 183 VRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
           VRKDGELVDFSYWKC++GLIRKNYPL+AESFILRHF+R
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLYAESFILRHFRR 218

[Close]
BLAST of Carg27331 vs. TrEMBL
Match: tr|A0A061FBM3|A0A061FBM3_THECC (Uncharacterized protein isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_033826 PE=4 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 5.1e-72
Identity = 135/216 (62.50%), Postives = 162/216 (75.00%), Query Frame = 0 

Query: 5   MASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRSHEEY 64
           MAS+LKPPP    + ++ +  ++SPVI+   +   TS      AL+TG +G RI S E Y
Sbjct: 1   MASVLKPPPYFHRNCISIS-SSSSPVILSSPSQRTTSLQVRSCALRTGSDGGRIGSQESY 60

Query: 65  SSDLLRKPVVPPAMDLATPSEDYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGFVRMV 124
            +D+LRKP +    D    SE                 W+DWEDRILE+TVPLVGFVRM+
Sbjct: 61  GADMLRKPSILTPKDSGGTSEQ----EEGSEGKRKRGKWIDWEDRILEDTVPLVGFVRMI 120

Query: 125 LHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCLFIVR 184
           +HSGKYESGDRL+PEHEKTILDRLL YHPECEKKIGCGIDYIT+GYHP+FE SRCLFIVR
Sbjct: 121 IHSGKYESGDRLSPEHEKTILDRLLPYHPECEKKIGCGIDYITVGYHPDFEGSRCLFIVR 180

Query: 185 KDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
           KDGEL+DFSYWKC++GLIRKNYPL+A+SFILRHF+R
Sbjct: 181 KDGELIDFSYWKCIKGLIRKNYPLYADSFILRHFRR 211

[Close]
BLAST of Carg27331 vs. TrEMBL
Match: tr|W9SA48|W9SA48_9ROSA (Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_009556 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.0e-69
Identity = 153/227 (67.40%), Postives = 176/227 (77.53%), Query Frame = 0 

Query: 5   MASILKPPPLV-PLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPE--GSRIRSH 64
           MA I  PPPL+  LH    +  + SPV + F     TSF   + ALKTG +  GSRI S 
Sbjct: 1   MAFISNPPPLLNNLHRHTLSHHHYSPVTLSFPFRATTSFPARVGALKTGSDGGGSRIGSQ 60

Query: 65  EEYSSDLLRKPVVPPAMDLATPSE--------DYSXXXXXXXXXXXXXXWVDWEDRILEE 124
           E +  DLLRKPVV P  DLA  SE           XXXXXXXXXXXXX WVDWED+ILE+
Sbjct: 61  ELFGPDLLRKPVVSPRKDLAGISEXXXXXXXXXXXXXXXXXXXXXXXXKWVDWEDKILED 120

Query: 125 TVPLVGFVRMVLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPN 184
           TVPLVGFVRM+LHS KYESGDRL+PEHEKTIL+RLL +HPE EKKIGCGIDYIT+GYHP+
Sbjct: 121 TVPLVGFVRMILHSEKYESGDRLSPEHEKTILERLLPFHPEFEKKIGCGIDYITVGYHPD 180

Query: 185 FERSRCLFIVRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
           FERSRCLFIV+KDG+LVDFSYWKC++GLIRKNYPL+A+SFILRHF++
Sbjct: 181 FERSRCLFIVQKDGKLVDFSYWKCIKGLIRKNYPLYADSFILRHFRQ 227

[Close]
BLAST of Carg27331 vs. TrEMBL
Match: tr|A0A2I4HX39|A0A2I4HX39_9ROSI (protein DCL, chloroplastic OS=Juglans regia OX=51240 GN=LOC109022277 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.5e-68
Identity = 137/217 (63.13%), Postives = 160/217 (73.73%), Query Frame = 0 

Query: 5   MASILKPPPLVPLH-SLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRSHEE 64
           MASI K  PL+ +H   NP+ L+ SPVI+ F +    S    +RAL+TGP+G        
Sbjct: 1   MASISK--PLLRVHLPTNPSPLHFSPVILSFPSFSKASLPRRLRALRTGPDG-------- 60

Query: 65  YSSDLLRKPVVPPAMDLATPSEDYSXXXXXXXXXXXXXXWVDWEDRILEETVPLVGFVRM 124
           Y S+LLRKPVV    D    S D                WVDWEDRILE+TVPLVGFVRM
Sbjct: 61  YGSELLRKPVVSQEKDSGGIS-DEGEENEEGKMRDYEEKWVDWEDRILEDTVPLVGFVRM 120

Query: 125 VLHSGKYESGDRLTPEHEKTILDRLLSYHPECEKKIGCGIDYITIGYHPNFERSRCLFIV 184
           ++HSGKY+SGDRL+ EHEKTIL+RLL +HPE EKKIGCGIDYITIGYHP FERSRCLFIV
Sbjct: 121 IIHSGKYQSGDRLSAEHEKTILERLLPFHPEFEKKIGCGIDYITIGYHPEFERSRCLFIV 180

Query: 185 RKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQR 221
           RKDGELVDFSYWKC++GLI+KNYPL+A+SFILRHFQ+
Sbjct: 181 RKDGELVDFSYWKCIKGLIKKNYPLYADSFILRHFQK 206

The following BLAST results are available for this feature:
BLAST of Carg27331 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022937348.12.7e-117100.00protein DCL homolog, chloroplastic-like [Cucurbita moschata][more]
XP_022975974.15.6e-11595.45protein DCL homolog, chloroplastic-like [Cucurbita maxima][more]
XP_023536599.14.7e-11495.91protein DCL homolog, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_004141259.13.8e-9580.73PREDICTED: protein DCL, chloroplastic [Cucumis sativus] >KGN55194.1 hypothetical... [more]
XP_008452556.14.2e-9481.19PREDICTED: protein DCL, chloroplastic [Cucumis melo][more]
BLAST of Carg27331 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G45230.12.7e-6361.54Protein of unknown function (DUF3223)[more]
AT3G46630.11.6e-3147.46Protein of unknown function (DUF3223)[more]
AT2G40030.13.0e-2235.96nuclear RNA polymerase D1B[more]
AT5G62440.11.6e-0742.37Protein of unknown function (DUF3223)[more]
BLAST of Carg27331 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q42463|DCL_SOLLC9.9e-6363.07Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1[more]
sp|Q9C642|DCL_ARATH4.9e-6261.54Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 S... [more]
sp|Q5D869|NRPE1_ARATH5.5e-2135.96DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1... [more]
sp|Q8L557|EM514_ARATH2.9e-0642.37Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1[more]
BLAST of Carg27331 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0L2D0|A0A0A0L2D0_CUCSA2.5e-9580.73Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639820 PE=4 SV=1[more]
tr|A0A1S3BVA4|A0A1S3BVA4_CUCME2.8e-9481.19protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493543 PE=4 SV=1[more]
tr|A0A061FBM3|A0A061FBM3_THECC5.1e-7262.50Uncharacterized protein isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_033826 PE=4 ... [more]
tr|W9SA48|W9SA48_9ROSA4.0e-6967.40Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_009556 PE=4 SV=1[more]
tr|A0A2I4HX39|A0A2I4HX39_9ROSI1.5e-6863.13protein DCL, chloroplastic OS=Juglans regia OX=51240 GN=LOC109022277 PE=4 SV=1[more]
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27331-RACarg27331-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF11523DUF3223coord: 123..198
e-value: 1.8E-27
score: 95.9
NoneNo IPR availableGENE3DG3DSA:3.10.450.40coord: 112..204
e-value: 1.1E-21
score: 78.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 79..102
NoneNo IPR availablePANTHERPTHR33415:SF2SUBFAMILY NOT NAMEDcoord: 6..224
NoneNo IPR availablePANTHERPTHR33415FAMILY NOT NAMEDcoord: 6..224