Cucsa.319460 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.319460 vs. Swiss-Prot
Match: PP252_ARATH (Pentatricopeptide repeat-containing protein At3g24000, mitochondrial OS=Arabidopsis thaliana GN=PCMP-H87 PE=3 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 6.9e-227
Identity = 374/581 (64.37%), Postives = 464/581 (79.86%), Query Frame = 1

Query: 30  GSLEP-ERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLE 89
           GS  P +R  Y+ +L KCT  + L QGR +HAHI  S F  D+V+ N +LNMYAKCGSLE
Sbjct: 53  GSYIPADRRFYNTLLKKCTVFKLLIQGRIVHAHILQSIFRHDIVMGNTLLNMYAKCGSLE 112

Query: 90  EAQDLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASG 149
           EA+ +F+KMP +D V+WT LISGYSQ  +  +AL  F +ML  G+ PNEFTLSS++KA+ 
Sbjct: 113 EARKVFEKMPQRDFVTWTTLISGYSQHDRPCDALLFFNQMLRFGYSPNEFTLSSVIKAAA 172

Query: 150 TGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNA 209
                  G QLH F +K G+D NVHVGS+LLD+Y R+  M +A+++F++L ++N VSWNA
Sbjct: 173 AERRGCCGHQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALESRNDVSWNA 232

Query: 210 LIAGHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSG 269
           LIAGHAR+   E  + LF  MLR GF P+HF+Y+S+F AC+S+G LEQGKWVHA++IKSG
Sbjct: 233 LIAGHARRSGTEKALELFQGMLRDGFRPSHFSYASLFGACSSTGFLEQGKWVHAYMIKSG 292

Query: 270 GQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFE 329
            + +A+ GNTL+DMYAKSGSI DA+K+F RL K+D+VSWNS+++ YAQHG G EA+  FE
Sbjct: 293 EKLVAFAGNTLLDMYAKSGSIHDARKIFDRLAKRDVVSWNSLLTAYAQHGFGKEAVWWFE 352

Query: 330 QMLKAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAG 389
           +M +  ++PNEI+FLSVLTACSHSGLLDEG +Y+ELMKK  I  +  H+VTVVDLLGRAG
Sbjct: 353 EMRRVGIRPNEISFLSVLTACSHSGLLDEGWHYYELMKKDGIVPEAWHYVTVVDLLGRAG 412

Query: 390 RLNEANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVYAAEQIFELDPHDSGPHVLLSN 449
            LN A +FIEEMPI+PTAA+W ALL ACRMHKN +LG YAAE +FELDP D GPHV+L N
Sbjct: 413 DLNRALRFIEEMPIEPTAAIWKALLNACRMHKNTELGAYAAEHVFELDPDDPGPHVILYN 472

Query: 450 IYASAGRLSDAAKVRKMMKESGVKKEPACSWVEIENEVHVFVANDDSHPMREEIQRMWEK 509
           IYAS GR +DAA+VRK MKESGVKKEPACSWVEIEN +H+FVAND+ HP REEI R WE+
Sbjct: 473 IYASGGRWNDAARVRKKMKESGVKKEPACSWVEIENAIHMFVANDERHPQREEIARKWEE 532

Query: 510 ISGKIKEIGYVPDTSHVLFFMNQQDRELKLQYHSEKLALAFAVLKTPPGLTIRIKKNIRI 569
           +  KIKE+GYVPDTSHV+  ++QQ+RE+ LQYHSEK+ALAFA+L TPPG TI IKKNIR+
Sbjct: 533 VLAKIKELGYVPDTSHVIVHVDQQEREVNLQYHSEKIALAFALLNTPPGSTIHIKKNIRV 592

Query: 570 CGDCHSAFKFASRVLGREIIVRDTNRFHHFLHGMCSCRDYW 610
           CGDCH+A K AS+V+GREIIVRDTNRFHHF  G CSC+DYW
Sbjct: 593 CGDCHTAIKLASKVVGREIIVRDTNRFHHFKDGNCSCKDYW 633

BLAST of Cucsa.319460 vs. Swiss-Prot
Match: PP227_ARATH (Putative pentatricopeptide repeat-containing protein At3g13770, mitochondrial OS=Arabidopsis thaliana GN=PCMP-H85 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.1e-139
Identity = 262/630 (41.59%), Postives = 395/630 (62.70%), Query Frame = 1

Query: 2   DLLRTTHISSSGTSSN-------------STGLYVLDLINCGSLEPERTL--YSKMLNKC 61
           +L+R  H S S + +N             S G     L+    L PE     Y  +LN C
Sbjct: 3   NLMRLIHRSFSSSPTNYVLQTILPISQLCSNGRLQEALLEMAMLGPEMGFHGYDALLNAC 62

Query: 62  TYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWT 121
              R L+ G+ +HAH+  + +     L   +L  Y KC  LE+A+ + D+MP K++VSWT
Sbjct: 63  LDKRALRDGQRVHAHMIKTRYLPATYLRTRLLIFYGKCDCLEDARKVLDEMPEKNVVSWT 122

Query: 122 VLISGYSQSGQASEALALFPKMLHLGFQPNEFT----LSSLLKASGTGPSDHHGRQLHAF 181
            +IS YSQ+G +SEAL +F +M+    +PNEFT    L+S ++ASG G     G+Q+H  
Sbjct: 123 AMISRYSQTGHSSEALTVFAEMMRSDGKPNEFTFATVLTSCIRASGLGL----GKQIHGL 182

Query: 182 SLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHV 241
            +K+ YD ++ VGSSLLDMYA+   ++EA+ IF  L  ++VVS  A+IAG+A+ G  E  
Sbjct: 183 IVKWNYDSHIFVGSSLLDMYAKAGQIKEAREIFECLPERDVVSCTAIIAGYAQLGLDEEA 242

Query: 242 MRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDM 301
           + +F ++  +G  P + TY+S+ TA +    L+ GK  H HV++      A + N+LIDM
Sbjct: 243 LEMFHRLHSEGMSPNYVTYASLLTALSGLALLDHGKQAHCHVLRRELPFYAVLQNSLIDM 302

Query: 302 YAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAK-VQPNEIT 361
           Y+K G++  A+++F  + ++  +SWN+++ GY++HGLG E L+LF  M   K V+P+ +T
Sbjct: 303 YSKCGNLSYARRLFDNMPERTAISWNAMLVGYSKHGLGREVLELFRLMRDEKRVKPDAVT 362

Query: 362 FLSVLTACSHSGLLDEGQYYFELMK--KHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEE 421
            L+VL+ CSH  + D G   F+ M   ++  +    H+  +VD+LGRAGR++EA +FI+ 
Sbjct: 363 LLAVLSGCSHGRMEDTGLNIFDGMVAGEYGTKPGTEHYGCIVDMLGRAGRIDEAFEFIKR 422

Query: 422 MPIKPTAAVWGALLGACRMHKNMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDA 481
           MP KPTA V G+LLGACR+H ++D+G     ++ E++P ++G +V+LSN+YASAGR +D 
Sbjct: 423 MPSKPTAGVLGSLLGACRVHLSVDIGESVGRRLIEIEPENAGNYVILSNLYASAGRWADV 482

Query: 482 AKVRKMMKESGVKKEPACSWVEIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYV 541
             VR MM +  V KEP  SW++ E  +H F AND +HP REE+    ++IS K+K+ GYV
Sbjct: 483 NNVRAMMMQKAVTKEPGRSWIQHEQTLHYFHANDRTHPRREEVLAKMKEISIKMKQAGYV 542

Query: 542 PDTSHVLFFMNQQDRELKLQYHSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFA 601
           PD S VL+ ++++ +E  L  HSEKLAL F ++ T  G+ IR+ KN+RIC DCH+  K  
Sbjct: 543 PDLSCVLYDVDEEQKEKMLLGHSEKLALTFGLIATGEGIPIRVFKNLRICVDCHNFAKIF 602

Query: 602 SRVLGREIIVRDTNRFHHFLHGMCSCRDYW 610
           S+V  RE+ +RD NRFH  + G+CSC DYW
Sbjct: 603 SKVFEREVSLRDKNRFHQIVDGICSCGDYW 628

BLAST of Cucsa.319460 vs. Swiss-Prot
Match: PPR32_ARATH (Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H40 PE=2 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 1.3e-137
Identity = 255/602 (42.36%), Postives = 366/602 (60.80%), Query Frame = 1

Query: 12  SGTSSNSTGLYVLDLINC---GSLEPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFE 71
           +G S N      L+++      +L+P       +L   + LR +  G+ IH +   S F+
Sbjct: 209 AGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFD 268

Query: 72  DDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKM 131
             + +   +++MYAKCGSLE A+ LFD M  +++VSW  +I  Y Q+    EA+ +F KM
Sbjct: 269 SLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKM 328

Query: 132 LHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHM 191
           L  G +P + ++   L A         GR +H  S++ G D NV V +SL+ MY +   +
Sbjct: 329 LDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEV 388

Query: 192 REAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTAC 251
             A  +F  L ++ +VSWNA+I G A+ G     +  F QM  +  +P  FTY SV TA 
Sbjct: 389 DTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAI 448

Query: 252 ASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWN 311
           A        KW+H  V++S      ++   L+DMYAK G+I  A+ +F  + ++ + +WN
Sbjct: 449 AELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWN 508

Query: 312 SIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKK- 371
           ++I GY  HG G  AL+LFE+M K  ++PN +TFLSV++ACSHSGL++ G   F +MK+ 
Sbjct: 509 AMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKEN 568

Query: 372 HKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVY 431
           + IE  + H+  +VDLLGRAGRLNEA  FI +MP+KP   V+GA+LGAC++HKN++    
Sbjct: 569 YSIELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEK 628

Query: 432 AAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWVEIENEVH 491
           AAE++FEL+P D G HVLL+NIY +A       +VR  M   G++K P CS VEI+NEVH
Sbjct: 629 AAERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGCSMVEIKNEVH 688

Query: 492 VFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQYHSEKLAL 551
            F +   +HP  ++I    EK+   IKE GYVPDT+ VL   N    +L L  HSEKLA+
Sbjct: 689 SFFSGSTAHPDSKKIYAFLEKLICHIKEAGYVPDTNLVLGVENDVKEQL-LSTHSEKLAI 748

Query: 552 AFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLHGMCSCRD 610
           +F +L T  G TI ++KN+R+C DCH+A K+ S V GREI+VRD  RFHHF +G CSC D
Sbjct: 749 SFGLLNTTAGTTIHVRKNLRVCADCHNATKYISLVTGREIVVRDMQRFHHFKNGACSCGD 808

HSP 2 Score: 227.3 bits (578), Expect = 4.7e-58
Identity = 132/442 (29.86%), Postives = 233/442 (52.71%), Query Frame = 1

Query: 32  LEPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQ 91
           +EP    ++ +L  C    +L+ G+ IH  +  S F  DL  +  + NMYAKC  + EA+
Sbjct: 131 VEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEAR 190

Query: 92  DLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGTGP 151
            +FD+MP +D+VSW  +++GYSQ+G A  AL +   M     +P+  T+ S+L A     
Sbjct: 191 KVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALR 250

Query: 152 SDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNALIA 211
               G+++H ++++ G+D  V++ ++L+DMYA+   +  A+ +F+ +  +NVVSWN++I 
Sbjct: 251 LISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMID 310

Query: 212 GHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQP 271
            + +    +  M +F +ML +G +PT  +      ACA  G LE+G+++H   ++ G   
Sbjct: 311 AYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDR 370

Query: 272 IAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQML 331
              + N+LI MY K   +  A  +F +L  + +VSWN++I G+AQ+G   +AL  F QM 
Sbjct: 371 NVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMR 430

Query: 332 KAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAGRLN 391
              V+P+  T++SV+TA +   +    ++   ++ +  ++  V     +VD+  + G + 
Sbjct: 431 SRTVKPDTFTYVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIM 490

Query: 392 EANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVYAAEQIFELDPHDSGPH--VLLSNI 451
            A + I +M  +     W A++     H     G  A E   E+      P+    LS I
Sbjct: 491 IA-RLIFDMMSERHVTTWNAMIDGYGTH---GFGKAALELFEEMQKGTIKPNGVTFLSVI 550

Query: 452 YA--SAGRLSDAAKVRKMMKES 470
            A   +G +    K   MMKE+
Sbjct: 551 SACSHSGLVEAGLKCFYMMKEN 568

HSP 3 Score: 178.3 bits (451), Expect = 2.5e-43
Identity = 93/376 (24.73%), Postives = 207/376 (55.05%), Query Frame = 1

Query: 42  MLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNF----ILNMYAKCGSLEEAQDLFDKM 101
           +L +C+ L++L+Q       I    F++ L   +F    +++++ + GS++EA  +F+ +
Sbjct: 43  LLERCSSLKELRQ-------ILPLVFKNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPI 102

Query: 102 PTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGR 161
            +K  V +  ++ G+++     +AL  F +M +   +P  +  + LLK  G       G+
Sbjct: 103 DSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGK 162

Query: 162 QLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKG 221
           ++H   +K G+ +++   + L +MYA+   + EA+ +F+ +  +++VSWN ++AG+++ G
Sbjct: 163 EIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNG 222

Query: 222 EGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGN 281
                + +   M  +  +P+  T  SV  A ++   +  GK +H + ++SG   +  I  
Sbjct: 223 MARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNIST 282

Query: 282 TLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQP 341
            L+DMYAK GS++ A+++F  ++++++VSWNS+I  Y Q+    EA+ +F++ML   V+P
Sbjct: 283 ALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKP 342

Query: 342 NEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFI 401
            +++ +  L AC+  G L+ G++  +L  +  ++  V+   +++ +  +   ++ A    
Sbjct: 343 TDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMF 402

Query: 402 EEMPIKPTAAVWGALL 414
            ++  + T   W A++
Sbjct: 403 GKLQSR-TLVSWNAMI 410

BLAST of Cucsa.319460 vs. Swiss-Prot
Match: PP307_ARATH (Pentatricopeptide repeat-containing protein At4g13650 OS=Arabidopsis thaliana GN=PCMP-H42 PE=2 SV=2)

HSP 1 Score: 479.6 bits (1233), Expect = 5.2e-134
Identity = 234/577 (40.55%), Postives = 363/577 (62.91%), Query Frame = 1

Query: 34   PERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDL 93
            P +  Y  +L  C  L  L+ G  IH+ I  + F+ +  + + +++MYAK G L+ A D+
Sbjct: 488  PNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTAWDI 547

Query: 94   FDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGTGPSD 153
              +   KD+VSWT +I+GY+Q     +AL  F +ML  G + +E  L++ + A     + 
Sbjct: 548  LIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAVSACAGLQAL 607

Query: 154  HHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNALIAGH 213
              G+Q+HA +   G+  ++   ++L+ +Y+R   + E+ + F    A + ++WNAL++G 
Sbjct: 608  KEGQQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEESYLAFEQTEAGDNIAWNALVSGF 667

Query: 214  ARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIA 273
             + G  E  +R+F++M R+G +  +FT+ S   A + + +++QGK VHA + K+G     
Sbjct: 668  QQSGNNEEALRVFVRMNREGIDNNNFTFGSAVKAASETANMKQGKQVHAVITKTGYDSET 727

Query: 274  YIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKA 333
             + N LI MYAK GSI DA+K F  +  ++ VSWN+II+ Y++HG G+EAL  F+QM+ +
Sbjct: 728  EVCNALISMYAKCGSISDAEKQFLEVSTKNEVSWNAIINAYSKHGFGSEALDSFDQMIHS 787

Query: 334  KVQPNEITFLSVLTACSHSGLLDEGQYYFELMK-KHKIEAQVAHHVTVVDLLGRAGRLNE 393
             V+PN +T + VL+ACSH GL+D+G  YFE M  ++ +  +  H+V VVD+L RAG L+ 
Sbjct: 788  NVRPNHVTLVGVLSACSHIGLVDKGIAYFESMNSEYGLSPKPEHYVCVVDMLTRAGLLSR 847

Query: 394  ANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVYAAEQIFELDPHDSGPHVLLSNIYAS 453
            A +FI+EMPIKP A VW  LL AC +HKNM++G +AA  + EL+P DS  +VLLSN+YA 
Sbjct: 848  AKEFIQEMPIKPDALVWRTLLSACVVHKNMEIGEFAAHHLLELEPEDSATYVLLSNLYAV 907

Query: 454  AGRLSDAAKVRKMMKESGVKKEPACSWVEIENEVHVFVANDDSHPMREEIQRMWEKISGK 513
            + +       R+ MKE GVKKEP  SW+E++N +H F   D +HP+ +EI   ++ ++ +
Sbjct: 908  SKKWDARDLTRQKMKEKGVKKEPGQSWIEVKNSIHSFYVGDQNHPLADEIHEYFQDLTKR 967

Query: 514  IKEIGYVPDTSHVLFFMNQQDRELKLQYHSEKLALAFAVLKTPPGLTIRIKKNIRICGDC 573
              EIGYV D   +L  +  + ++  +  HSEKLA++F +L  P  + I + KN+R+C DC
Sbjct: 968  ASEIGYVQDCFSLLNELQHEQKDPIIFIHSEKLAISFGLLSLPATVPINVMKNLRVCNDC 1027

Query: 574  HSAFKFASRVLGREIIVRDTNRFHHFLHGMCSCRDYW 610
            H+  KF S+V  REIIVRD  RFHHF  G CSC+DYW
Sbjct: 1028 HAWIKFVSKVSNREIIVRDAYRFHHFEGGACSCKDYW 1064

HSP 2 Score: 208.0 bits (528), Expect = 2.9e-52
Identity = 119/401 (29.68%), Postives = 202/401 (50.37%), Query Frame = 1

Query: 21  LYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNM 80
           +YVL ++      P    +S +L+ C  +  L+ G  +H  +    F  D  + N ++++
Sbjct: 279 MYVLGIM------PTPYAFSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNALVSL 338

Query: 81  YAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTL 140
           Y   G+L  A+ +F  M  +D V++  LI+G SQ G   +A+ LF +M   G +P+  TL
Sbjct: 339 YFHLGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPDSNTL 398

Query: 141 SSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAA 200
           +SL+ A     +   G+QLHA++ K G+  N  +  +LL++YA+ A +  A   F     
Sbjct: 399 ASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFLETEV 458

Query: 201 KNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWV 260
           +NVV WN ++  +    +  +  R+F QM  +   P  +TY S+   C   G LE G+ +
Sbjct: 459 ENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLGDLELGEQI 518

Query: 261 HAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLG 320
           H+ +IK+  Q  AY+ + LIDMYAK G +  A  +  R   +D+VSW ++I+GY Q+   
Sbjct: 519 HSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTAWDILIRFAGKDVVSWTTMIAGYTQYNFD 578

Query: 321 AEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTV 380
            +AL  F QML   ++ +E+   + ++AC+    L EGQ            + +     +
Sbjct: 579 DKALTTFRQMLDRGIRSDEVGLTNAVSACAGLQALKEGQQIHAQACVSGFSSDLPFQNAL 638

Query: 381 VDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHKN 422
           V L  R G++ E+    E+       A W AL+   +   N
Sbjct: 639 VTLYSRCGKIEESYLAFEQTEAGDNIA-WNALVSGFQQSGN 672

HSP 3 Score: 181.8 bits (460), Expect = 2.3e-44
Identity = 114/449 (25.39%), Postives = 214/449 (47.66%), Query Frame = 1

Query: 32  LEPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQ 91
           LEP+    + ++  C+    L +G+ +HA+     F  +  +   +LN+YAKC  +E A 
Sbjct: 385 LEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETAL 444

Query: 92  DLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGTGP 151
           D F +   +++V W V++  Y        +  +F +M      PN++T  S+LK      
Sbjct: 445 DYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLG 504

Query: 152 SDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNALIA 211
               G Q+H+  +K  + +N +V S L+DMYA+   +  A  I    A K+VVSW  +IA
Sbjct: 505 DLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTAWDILIRFAGKDVVSWTTMIA 564

Query: 212 GHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQP 271
           G+ +    +  +  F QML +G        ++  +ACA   +L++G+ +HA    SG   
Sbjct: 565 GYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAVSACAGLQALKEGQQIHAQACVSGFSS 624

Query: 272 IAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQML 331
                N L+ +Y++ G I+++   F +    D ++WN+++SG+ Q G   EAL++F +M 
Sbjct: 625 DLPFQNALVTLYSRCGKIEESYLAFEQTEAGDNIAWNALVSGFQQSGNNEEALRVFVRMN 684

Query: 332 KAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAGRLN 391
           +  +  N  TF S + A S +  + +G+    ++ K   +++      ++ +  + G ++
Sbjct: 685 REGIDNNNFTFGSAVKAASETANMKQGKQVHAVITKTGYDSETEVCNALISMYAKCGSIS 744

Query: 392 EANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVYAAEQIFELDPHDSGP-HVLLSNIY 451
           +A K   E+  K   + W A++ A   H     G  A +   ++   +  P HV L  + 
Sbjct: 745 DAEKQFLEVSTKNEVS-WNAIINAYSKH---GFGSEALDSFDQMIHSNVRPNHVTLVGVL 804

Query: 452 ASAGRL----SDAAKVRKMMKESGVKKEP 476
           ++   +       A    M  E G+  +P
Sbjct: 805 SACSHIGLVDKGIAYFESMNSEYGLSPKP 829

HSP 4 Score: 174.5 bits (441), Expect = 3.6e-42
Identity = 101/336 (30.06%), Postives = 177/336 (52.68%), Query Frame = 1

Query: 58  IHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQ 117
           IHA I      D  V+ N ++++Y++ G ++ A+ +FD +  KD  SW  +ISG S++  
Sbjct: 209 IHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDGLRLKDHSSWVAMISGLSKNEC 268

Query: 118 ASEALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSS 177
            +EA+ LF  M  LG  P  +  SS+L A     S   G QLH   LK G+  + +V ++
Sbjct: 269 EAEAIRLFCDMYVLGIMPTPYAFSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNA 328

Query: 178 LLDMYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPT 237
           L+ +Y    ++  A+ IF++++ ++ V++N LI G ++ G GE  M LF +M   G EP 
Sbjct: 329 LVSLYFHLGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPD 388

Query: 238 HFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFR 297
             T +S+  AC++ G+L +G+ +HA+  K G      I   L+++YAK   I+ A   F 
Sbjct: 389 SNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFL 448

Query: 298 RLVKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDE 357
               +++V WN ++  Y        + ++F QM   ++ PN+ T+ S+L  C   G L+ 
Sbjct: 449 ETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLGDLEL 508

Query: 358 GQYYFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEA 394
           G+     + K   +        ++D+  + G+L+ A
Sbjct: 509 GEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTA 544

HSP 5 Score: 163.3 bits (412), Expect = 8.3e-39
Identity = 111/419 (26.49%), Postives = 200/419 (47.73%), Query Frame = 1

Query: 24  LDLINCGSLEPERTLYSKMLNKCTYLR-KLKQGRAIHAHIQSSTFEDDLVLLNFILNMYA 83
           +D +    + P       +L  C      L +GR +H+ I     + +  L   + + Y 
Sbjct: 72  IDSVENRGIRPNHQTLKWLLEGCLKTNGSLDEGRKLHSQILKLGLDSNGCLSEKLFDFYL 131

Query: 84  KCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSS 143
             G L  A  +FD+MP + + +W  +I   +      E   LF +M+     PNE T S 
Sbjct: 132 FKGDLYGAFKVFDEMPERTIFTWNKMIKELASRNLIGEVFGLFVRMVSENVTPNEGTFSG 191

Query: 144 LLKAS-GTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAK 203
           +L+A  G   +     Q+HA  L  G   +  V + L+D+Y+R   +  A+ +F+ L  K
Sbjct: 192 VLEACRGGSVAFDVVEQIHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDGLRLK 251

Query: 204 NVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVH 263
           +  SW A+I+G ++       +RLF  M   G  PT + +SSV +AC    SLE G+ +H
Sbjct: 252 DHSSWVAMISGLSKNECEAEAIRLFCDMYVLGIMPTPYAFSSVLSACKKIESLEIGEQLH 311

Query: 264 AHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGA 323
             V+K G     Y+ N L+ +Y   G++  A+ +F  + ++D V++N++I+G +Q G G 
Sbjct: 312 GLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGE 371

Query: 324 EALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVV 383
           +A++LF++M    ++P+  T  S++ ACS  G L  GQ       K    +       ++
Sbjct: 372 KAMELFKRMHLDGLEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALL 431

Query: 384 DLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHKNM--DLGVYAAEQIFELDPH 439
           +L  +   +  A  +  E  ++    +W  +L A  +  ++     ++   QI E+ P+
Sbjct: 432 NLYAKCADIETALDYFLETEVE-NVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPN 489

BLAST of Cucsa.319460 vs. Swiss-Prot
Match: PP224_ARATH (Pentatricopeptide repeat-containing protein At3g12770 OS=Arabidopsis thaliana GN=PCMP-H43 PE=2 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 8.9e-134
Identity = 231/580 (39.83%), Postives = 366/580 (63.10%), Query Frame = 1

Query: 32  LEPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQ 91
           + P+   +  +L  C+ L  L+ GR +HA +    F+ D+ + N ++ +YAKC  L  A+
Sbjct: 115 VSPDSFTFPHLLKACSGLSHLQMGRFVHAQVFRLGFDADVFVQNGLIALYAKCRRLGSAR 174

Query: 92  DLFD--KMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGT 151
            +F+   +P + +VSWT ++S Y+Q+G+  EAL +F +M  +  +P+   L S+L A   
Sbjct: 175 TVFEGLPLPERTIVSWTAIVSAYAQNGEPMEALEIFSQMRKMDVKPDWVALVSVLNAFTC 234

Query: 152 GPSDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNAL 211
                 GR +HA  +K G ++   +  SL  MYA+   +  AK++F+ + + N++ WNA+
Sbjct: 235 LQDLKQGRSIHASVVKMGLEIEPDLLISLNTMYAKCGQVATAKILFDKMKSPNLILWNAM 294

Query: 212 IAGHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGG 271
           I+G+A+ G     + +F +M+ +   P   + +S  +ACA  GSLEQ + ++ +V +S  
Sbjct: 295 ISGYAKNGYAREAIDMFHEMINKDVRPDTISITSAISACAQVGSLEQARSMYEYVGRSDY 354

Query: 272 QPIAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQ 331
           +   +I + LIDM+AK GS++ A+ VF R + +D+V W+++I GY  HG   EA+ L+  
Sbjct: 355 RDDVFISSALIDMFAKCGSVEGARLVFDRTLDRDVVVWSAMIVGYGLHGRAREAISLYRA 414

Query: 332 MLKAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAGR 391
           M +  V PN++TFL +L AC+HSG++ EG ++F  M  HKI  Q  H+  V+DLLGRAG 
Sbjct: 415 MERGGVHPNDVTFLGLLMACNHSGMVREGWWFFNRMADHKINPQQQHYACVIDLLGRAGH 474

Query: 392 LNEANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVYAAEQIFELDPHDSGPHVLLSNI 451
           L++A + I+ MP++P   VWGALL AC+ H++++LG YAA+Q+F +DP ++G +V LSN+
Sbjct: 475 LDQAYEVIKCMPVQPGVTVWGALLSACKKHRHVELGEYAAQQLFSIDPSNTGHYVQLSNL 534

Query: 452 YASAGRLSDAAKVRKMMKESGVKKEPACSWVEIENEVHVFVANDDSHPMREEIQRMWEKI 511
           YA+A      A+VR  MKE G+ K+  CSWVE+   +  F   D SHP  EEI+R  E I
Sbjct: 535 YAAARLWDRVAEVRVRMKEKGLNKDVGCSWVEVRGRLEAFRVGDKSHPRYEEIERQVEWI 594

Query: 512 SGKIKEIGYVPDTSHVLFFMNQQDRELKLQYHSEKLALAFAVLKTPPGLTIRIKKNIRIC 571
             ++KE G+V +    L  +N ++ E  L  HSE++A+A+ ++ TP G  +RI KN+R C
Sbjct: 595 ESRLKEGGFVANKDASLHDLNDEEAEETLCSHSERIAIAYGLISTPQGTPLRITKNLRAC 654

Query: 572 GDCHSAFKFASRVLGREIIVRDTNRFHHFLHGMCSCRDYW 610
            +CH+A K  S+++ REI+VRDTNRFHHF  G+CSC DYW
Sbjct: 655 VNCHAATKLISKLVDREIVVRDTNRFHHFKDGVCSCGDYW 694

HSP 2 Score: 187.2 bits (474), Expect = 5.4e-46
Identity = 107/407 (26.29%), Postives = 206/407 (50.61%), Query Frame = 1

Query: 37  TLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDK 96
           + Y+ +++  T+  +LKQ   IHA +     +    L+  +++  +  G +  A+ +FD 
Sbjct: 22  SFYASLIDSATHKAQLKQ---IHARLLVLGLQFSGFLITKLIHASSSFGDITFARQVFDD 81

Query: 97  MPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHG 156
           +P   +  W  +I GYS++    +AL ++  M      P+ FT   LLKA         G
Sbjct: 82  LPRPQIFPWNAIIRGYSRNNHFQDALLMYSNMQLARVSPDSFTFPHLLKACSGLSHLQMG 141

Query: 157 RQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNS--LAAKNVVSWNALIAGHA 216
           R +HA   + G+D +V V + L+ +YA+   +  A+ +F    L  + +VSW A+++ +A
Sbjct: 142 RFVHAQVFRLGFDADVFVQNGLIALYAKCRRLGSARTVFEGLPLPERTIVSWTAIVSAYA 201

Query: 217 RKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAY 276
           + GE    + +F QM +   +P      SV  A      L+QG+ +HA V+K G +    
Sbjct: 202 QNGEPMEALEIFSQMRKMDVKPDWVALVSVLNAFTCLQDLKQGRSIHASVVKMGLEIEPD 261

Query: 277 IGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAK 336
           +  +L  MYAK G +  AK +F ++   +++ WN++ISGYA++G   EA+ +F +M+   
Sbjct: 262 LLISLNTMYAKCGQVATAKILFDKMKSPNLILWNAMISGYAKNGYAREAIDMFHEMINKD 321

Query: 337 VQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEAN 396
           V+P+ I+  S ++AC+  G L++ +  +E + +      V     ++D+  + G + E  
Sbjct: 322 VRPDTISITSAISACAQVGSLEQARSMYEYVGRSDYRDDVFISSALIDMFAKCGSV-EGA 381

Query: 397 KFIEEMPIKPTAAVWGALLGACRMH--KNMDLGVYAAEQIFELDPHD 440
           + + +  +     VW A++    +H      + +Y A +   + P+D
Sbjct: 382 RLVFDRTLDRDVVVWSAMIVGYGLHGRAREAISLYRAMERGGVHPND 424

BLAST of Cucsa.319460 vs. TrEMBL
Match: A0A0A0LSV8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171070 PE=4 SV=1)

HSP 1 Score: 1231.1 bits (3184), Expect = 0.0e+00
Identity = 608/609 (99.84%), Postives = 609/609 (100.00%), Query Frame = 1

Query: 1   EDLLRTTHISSSGTSSNSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA 60
           EDLLRTTHISSSGTSSNSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA
Sbjct: 70  EDLLRTTHISSSGTSSNSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA 129

Query: 61  HIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASE 120
           HIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASE
Sbjct: 130 HIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASE 189

Query: 121 ALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLD 180
           ALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLD
Sbjct: 190 ALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLD 249

Query: 181 MYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFT 240
           MYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFT
Sbjct: 250 MYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFT 309

Query: 241 YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLV 300
           YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLV
Sbjct: 310 YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLV 369

Query: 301 KQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQY 360
           KQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQY
Sbjct: 370 KQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQY 429

Query: 361 YFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHK 420
           YFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLG+CRMHK
Sbjct: 430 YFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGSCRMHK 489

Query: 421 NMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWV 480
           NMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWV
Sbjct: 490 NMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWV 549

Query: 481 EIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQY 540
           EIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQY
Sbjct: 550 EIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQY 609

Query: 541 HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLH 600
           HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLH
Sbjct: 610 HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLH 669

Query: 601 GMCSCRDYW 610
           GMCSCRDYW
Sbjct: 670 GMCSCRDYW 678

BLAST of Cucsa.319460 vs. TrEMBL
Match: M5X1J8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023254mg PE=4 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 2.3e-253
Identity = 415/561 (73.98%), Postives = 496/561 (88.41%), Query Frame = 1

Query: 51  KLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLIS 110
           +L+Q R +HAHI +S F+DDL + N ILNMY KCGSLE+A++LFD+MP+KD+V+WT LIS
Sbjct: 3   RLQQARIVHAHILTSHFKDDLPIHNTILNMYVKCGSLEDARNLFDQMPSKDLVTWTALIS 62

Query: 111 GYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDH--HGRQLHAFSLKYGY 170
           GYSQ  +  +AL LFP+ML  G +PN+FTLSSL KA+G    D+  HGRQLHA+ LKYG+
Sbjct: 63  GYSQYDRPQDALVLFPQMLLRGLEPNQFTLSSLFKAAGAVSDDNNKHGRQLHAYCLKYGF 122

Query: 171 DMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQ 230
           D NV+VG+SL+DMYARW HM E+++IF+SL  KN VSWNALIAGHARK +GEH +RLF +
Sbjct: 123 DTNVYVGTSLVDMYARWGHMDESQLIFDSLETKNEVSWNALIAGHARKAQGEHALRLFWK 182

Query: 231 MLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGS 290
           MLR+GF+PTHFTYSSVFTACAS+GS+EQGKWVHAH+IKSG + +A++GNTL+DMYAKSGS
Sbjct: 183 MLREGFKPTHFTYSSVFTACASAGSMEQGKWVHAHMIKSGAKLVAFVGNTLLDMYAKSGS 242

Query: 291 IKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTA 350
           I+DA+KVF RLV+QDIVSWNS+++GYAQHGLG E +Q FE+ML+  +QPN+ITFL VLTA
Sbjct: 243 IEDARKVFDRLVRQDIVSWNSMLTGYAQHGLGQETVQRFEEMLRIGIQPNDITFLCVLTA 302

Query: 351 CSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAV 410
           CSH+GLLDEGQYYF+LMK + IE Q++H+VT+VDLLGRAG L+ A KFI EMPI+PTAAV
Sbjct: 303 CSHAGLLDEGQYYFDLMKSYNIELQISHYVTIVDLLGRAGLLDRAAKFIREMPIEPTAAV 362

Query: 411 WGALLGACRMHKNMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKE 470
           WGALLGACRMHKN+DLG YAAE++FELDPHDSGPHVLLSNIYASAGRLSDAA+VRK+MK+
Sbjct: 363 WGALLGACRMHKNIDLGAYAAERVFELDPHDSGPHVLLSNIYASAGRLSDAARVRKLMKD 422

Query: 471 SGVKKEPACSWVEIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFF 530
            GVKKEPACSWVEIEN VH+FVANDD+HP R EI +MWE ISGKIK+IGYVPDTSHVLFF
Sbjct: 423 CGVKKEPACSWVEIENAVHMFVANDDAHPQRVEILQMWETISGKIKDIGYVPDTSHVLFF 482

Query: 531 MNQQDRELKLQYHSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREII 590
           ++QQ+RE+KLQYHSEKLALAFA+L T PG TIRIKKNIR+CGDCHSA K+ S+V GREII
Sbjct: 483 VDQQEREVKLQYHSEKLALAFALLNTTPGSTIRIKKNIRVCGDCHSAIKYVSKVEGREII 542

Query: 591 VRDTNRFHHFLHGMCSCRDYW 610
           VRDTNRFHHF +G CSCRDYW
Sbjct: 543 VRDTNRFHHFRNGSCSCRDYW 563

BLAST of Cucsa.319460 vs. TrEMBL
Match: M5X1J8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023254mg PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 4.0e-16
Identity = 52/179 (29.05%), Postives = 90/179 (50.28%), Query Frame = 1

Query: 33  EPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQD 92
           +P    YS +   C     ++QG+ +HAH+  S  +    + N +L+MYAK GS+E+A+ 
Sbjct: 189 KPTHFTYSSVFTACASAGSMEQGKWVHAHMIKSGAKLVAFVGNTLLDMYAKSGSIEDARK 248

Query: 93  LFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGTGPS 152
           +FD++  +D+VSW  +++GY+Q G   E +  F +ML +G QPN+ T   +L A      
Sbjct: 249 VFDRLVRQDIVSWNSMLTGYAQHGLGQETVQRFEEMLRIGIQPNDITFLCVLTACSHAGL 308

Query: 153 DHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHM-REAKVIFNSLAAKNVVSWNALI 211
              G+        Y  ++ +    +++D+  R   + R AK I           W AL+
Sbjct: 309 LDEGQYYFDLMKSYNIELQISHYVTIVDLLGRAGLLDRAAKFIREMPIEPTAAVWGALL 367

HSP 2 Score: 870.5 bits (2248), Expect = 1.2e-249
Identity = 421/597 (70.52%), Postives = 498/597 (83.42%), Query Frame = 1

Query: 17  NSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNF 76
           + TGL+VLDL++ GSLEP R LY  +L KCT LRK K G+ +HAHI +S F  DLV+LN 
Sbjct: 79  SGTGLHVLDLLDQGSLEPHRALYHLLLKKCTRLRKPKLGKIVHAHILNSLFRHDLVILNS 138

Query: 77  ILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPN 136
           +LNMYAKCG L++A++LFD+MP KDMVSWT LI+ YSQ G+   A+ LFP ML LGF PN
Sbjct: 139 LLNMYAKCGCLDDARNLFDQMPVKDMVSWTTLITAYSQHGRPFHAILLFPHMLSLGFFPN 198

Query: 137 EFTLSSLLKASGTGPSD----HHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAK 196
           +FTLSSLLKAS   P       HG QLHAF  K G   NV+VGSSLLDMYAR   M EA+
Sbjct: 199 QFTLSSLLKASAALPDTPHIHFHGTQLHAFCFKCGLHSNVYVGSSLLDMYARCGCMDEAR 258

Query: 197 VIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSG 256
           +IF++L +KN VSWNALIAGHARKG+ +H + LF +MLRQ F+PTHFT+ S+  ACAS+G
Sbjct: 259 LIFDALHSKNEVSWNALIAGHARKGQADHALWLFRKMLRQDFQPTHFTFCSILGACASTG 318

Query: 257 SLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIIS 316
           SLEQGKWVHAHVIKSGG+ +A++GNTL+ MYAKSGSI+DAK +F RLVK D+VSWNS+++
Sbjct: 319 SLEQGKWVHAHVIKSGGEIVAFMGNTLLHMYAKSGSIQDAKMIFDRLVKSDVVSWNSMLT 378

Query: 317 GYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEA 376
            YAQHGLG EA+Q F++ML+  V PN+ITFL +LTACS +GLLD+GQYYF+LM  + IE 
Sbjct: 379 AYAQHGLGKEAVQWFQEMLRIGVGPNDITFLCLLTACSRAGLLDQGQYYFKLMSNYNIEP 438

Query: 377 QVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVYAAEQI 436
           Q++H+VT+VDL GRAG L++A +FI EMPIKPTAAVWGALLGACRMHKNM+LG YAAE++
Sbjct: 439 QISHYVTIVDLFGRAGLLDKAERFIREMPIKPTAAVWGALLGACRMHKNMELGTYAAERV 498

Query: 437 FELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWVEIENEVHVFVAN 496
           FELDPHDSGP VLLSNIYASAGR SDAAKVRKMMKESGVKKEPACSWVEIEN VHVFVAN
Sbjct: 499 FELDPHDSGPLVLLSNIYASAGRWSDAAKVRKMMKESGVKKEPACSWVEIENTVHVFVAN 558

Query: 497 DDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQYHSEKLALAFAVL 556
           DD+HP  EEI +MWE+IS +IKEIGYVPD SHVLF+M+QQ+RE+KLQYHSEKLALAFA+L
Sbjct: 559 DDAHPQMEEIHKMWEEISARIKEIGYVPDISHVLFYMDQQEREIKLQYHSEKLALAFALL 618

Query: 557 KTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLHGMCSCRDYW 610
            + PG TIRIKKNIR+CGDCHSA KF S+V+ REIIVRDTNRFHHF  G CSC DYW
Sbjct: 619 NSSPGSTIRIKKNIRVCGDCHSAIKFVSQVVRREIIVRDTNRFHHFRGGSCSCGDYW 675

BLAST of Cucsa.319460 vs. TrEMBL
Match: F6GZB2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0455g00030 PE=4 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 1.0e-245
Identity = 420/610 (68.85%), Postives = 499/610 (81.80%), Query Frame = 1

Query: 1   EDLLRTTHISSSGTSSNSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA 60
           +DLLR +       S   TGLY LDLI  GSL P+  LYSK+L +CT L K++QGR +HA
Sbjct: 58  KDLLRKSQ------SDGGTGLYALDLIQRGSLVPDYNLYSKLLKECTRLGKVEQGRIVHA 117

Query: 61  HIQSSTFEDD-LVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQAS 120
           H+  S F D+ LVL N I+NMYAKCG L++A+ +FD+MPTKDMV+WT LI+G+SQ+ +  
Sbjct: 118 HLVDSHFLDNHLVLQNIIVNMYAKCGCLDDARRMFDEMPTKDMVTWTALIAGFSQNNRPR 177

Query: 121 EALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLL 180
           +AL LFP+ML LGFQPN FTLSSLLKASG+      G QLHAF LKYGY  +V+VGS+L+
Sbjct: 178 DALLLFPQMLRLGFQPNHFTLSSLLKASGSEHGLDPGTQLHAFCLKYGYQSSVYVGSALV 237

Query: 181 DMYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHF 240
           DMYAR  HM  A++ F+ +  K+ VSWNALI+GHARKGEGEH + L  +M R+ F+PTHF
Sbjct: 238 DMYARCGHMDAAQLAFDGMPTKSEVSWNALISGHARKGEGEHALHLLWKMQRKNFQPTHF 297

Query: 241 TYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRL 300
           TYSSV +ACAS G+LEQGKWVHAH+IKSG + IA+IGNTL+DMYAK+GSI DAK+VF RL
Sbjct: 298 TYSSVLSACASIGALEQGKWVHAHMIKSGLKLIAFIGNTLLDMYAKAGSIDDAKRVFDRL 357

Query: 301 VKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQ 360
           VK D+VSWN++++G AQHGLG E L  FEQML+  ++PNEI+FL VLTACSHSGLLDEG 
Sbjct: 358 VKPDVVSWNTMLTGCAQHGLGKETLDRFEQMLRIGIEPNEISFLCVLTACSHSGLLDEGL 417

Query: 361 YYFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMH 420
           YYFELMKK+K+E  V H+VT VDLLGR G L+ A +FI EMPI+PTAAVWGALLGACRMH
Sbjct: 418 YYFELMKKYKVEPDVPHYVTFVDLLGRVGLLDRAERFIREMPIEPTAAVWGALLGACRMH 477

Query: 421 KNMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSW 480
           KNM+LGVYAAE+ FELDPHDSGP +LLSNIYASAGR  D AKVRKMMKESGVKK+PACSW
Sbjct: 478 KNMELGVYAAERAFELDPHDSGPRMLLSNIYASAGRWRDVAKVRKMMKESGVKKQPACSW 537

Query: 481 VEIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQ 540
           VEIEN VH+FVAND++HP  +EI+  WE+ISGKIKEIGYVPDTSHVL F++QQ+RE KLQ
Sbjct: 538 VEIENAVHLFVANDETHPRIKEIRGKWEEISGKIKEIGYVPDTSHVLLFVDQQEREEKLQ 597

Query: 541 YHSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFL 600
           YHSEKLALAFA+L TP G  IRIKKNIR+CGDCH+A KF S+V+ REIIVRDTNRFH F 
Sbjct: 598 YHSEKLALAFALLNTPTGSPIRIKKNIRVCGDCHAAIKFVSKVVDREIIVRDTNRFHRFR 657

Query: 601 HGMCSCRDYW 610
            G CSC DYW
Sbjct: 658 DGSCSCGDYW 661

BLAST of Cucsa.319460 vs. TrEMBL
Match: A0A0D2NHM0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G032400 PE=4 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 3.1e-242
Identity = 408/597 (68.34%), Postives = 495/597 (82.91%), Query Frame = 1

Query: 17  NSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNF 76
           + TGL+VLDL++ GSLEP  TLY  +L KCT+LRK K G+ +HAHI +S F  D+V+LN 
Sbjct: 62  SGTGLHVLDLVDEGSLEPHPTLYHLLLKKCTFLRKPKLGKIVHAHISNSHFRGDVVILNT 121

Query: 77  ILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPN 136
           +LNMYAKCG L++A++LFD+M  KDMV+WT LI+ YSQ  +  +AL LFP+ML LGF PN
Sbjct: 122 LLNMYAKCGCLDDARNLFDEMSVKDMVTWTTLITAYSQHDRPLDALLLFPQMLSLGFFPN 181

Query: 137 EFTLSSLLKASG----TGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAK 196
           EFTLSSLLKAS     T     HG QLH FS K G+  NV+VGSSLLDMYAR   M EA 
Sbjct: 182 EFTLSSLLKASAALSDTPQIYFHGAQLHGFSFKCGFHSNVYVGSSLLDMYARCGCMDEAH 241

Query: 197 VIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSG 256
            +F+ L  KN VSWNALIA HARKG+ +  + LFL+MLRQ F+PTHFT+SS+F ACAS+G
Sbjct: 242 FVFDVLPTKNEVSWNALIADHARKGQLDRALSLFLKMLRQDFQPTHFTFSSIFGACASTG 301

Query: 257 SLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIIS 316
           SLEQGKWVHAHVIKSGG+ IA++GNTL+ MYAKSGSI+DAK +F RLVK+D+VSWNS+++
Sbjct: 302 SLEQGKWVHAHVIKSGGEIIAFMGNTLLHMYAKSGSIQDAKMIFDRLVKRDLVSWNSMLT 361

Query: 317 GYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEA 376
            YAQHGLG EA+Q F++ML   + PN+ITFL +LTACS +GLL+EGQYYF+ M ++ IE 
Sbjct: 362 AYAQHGLGKEAVQCFQKMLNIGLVPNDITFLCLLTACSRAGLLEEGQYYFKSMNRYNIEP 421

Query: 377 QVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVYAAEQI 436
            ++H+VT+VDLLGRAG LNEA +FI EMPI+PTAAVWGALLGACRMHKN+++G +AAE++
Sbjct: 422 GISHYVTIVDLLGRAGLLNEALRFINEMPIQPTAAVWGALLGACRMHKNVEMGSFAAERV 481

Query: 437 FELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWVEIENEVHVFVAN 496
           FELDPHDSGPHVLLSNIYAS  R  DAAKVRK+MKESGVKKEPACSWVEI+N VH+FVA+
Sbjct: 482 FELDPHDSGPHVLLSNIYASGNRWRDAAKVRKLMKESGVKKEPACSWVEIQNTVHMFVAS 541

Query: 497 DDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQYHSEKLALAFAVL 556
           DDSHP    I + WE+IS +IKEIGYVPDTSHVLF+M++Q+RE+KLQYHSEKLALAFA+L
Sbjct: 542 DDSHPQMGAIYKKWEEISARIKEIGYVPDTSHVLFYMDEQEREVKLQYHSEKLALAFALL 601

Query: 557 KTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLHGMCSCRDYW 610
            +P G TIRIKKNIR+CGDCHSAFKF S+V+GREIIVRDTNRFHHF  G CSC ++W
Sbjct: 602 NSPDGSTIRIKKNIRVCGDCHSAFKFVSKVVGREIIVRDTNRFHHFYDGSCSCGNFW 658

BLAST of Cucsa.319460 vs. TAIR10
Match: AT3G24000.1 (AT3G24000.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 769.2 bits (1985), Expect = 1.9e-222
Identity = 367/570 (64.39%), Postives = 456/570 (80.00%), Query Frame = 1

Query: 30  GSLEP-ERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLE 89
           GS  P +R  Y+ +L KCT  + L QGR +HAHI  S F  D+V+ N +LNMYAKCGSLE
Sbjct: 53  GSYIPADRRFYNTLLKKCTVFKLLIQGRIVHAHILQSIFRHDIVMGNTLLNMYAKCGSLE 112

Query: 90  EAQDLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASG 149
           EA+ +F+KMP +D V+WT LISGYSQ  +  +AL  F +ML  G+ PNEFTLSS++KA+ 
Sbjct: 113 EARKVFEKMPQRDFVTWTTLISGYSQHDRPCDALLFFNQMLRFGYSPNEFTLSSVIKAAA 172

Query: 150 TGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNA 209
                  G QLH F +K G+D NVHVGS+LLD+Y R+  M +A+++F++L ++N VSWNA
Sbjct: 173 AERRGCCGHQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALESRNDVSWNA 232

Query: 210 LIAGHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSG 269
           LIAGHAR+   E  + LF  MLR GF P+HF+Y+S+F AC+S+G LEQGKWVHA++IKSG
Sbjct: 233 LIAGHARRSGTEKALELFQGMLRDGFRPSHFSYASLFGACSSTGFLEQGKWVHAYMIKSG 292

Query: 270 GQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFE 329
            + +A+ GNTL+DMYAKSGSI DA+K+F RL K+D+VSWNS+++ YAQHG G EA+  FE
Sbjct: 293 EKLVAFAGNTLLDMYAKSGSIHDARKIFDRLAKRDVVSWNSLLTAYAQHGFGKEAVWWFE 352

Query: 330 QMLKAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAG 389
           +M +  ++PNEI+FLSVLTACSHSGLLDEG +Y+ELMKK  I  +  H+VTVVDLLGRAG
Sbjct: 353 EMRRVGIRPNEISFLSVLTACSHSGLLDEGWHYYELMKKDGIVPEAWHYVTVVDLLGRAG 412

Query: 390 RLNEANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVYAAEQIFELDPHDSGPHVLLSN 449
            LN A +FIEEMPI+PTAA+W ALL ACRMHKN +LG YAAE +FELDP D GPHV+L N
Sbjct: 413 DLNRALRFIEEMPIEPTAAIWKALLNACRMHKNTELGAYAAEHVFELDPDDPGPHVILYN 472

Query: 450 IYASAGRLSDAAKVRKMMKESGVKKEPACSWVEIENEVHVFVANDDSHPMREEIQRMWEK 509
           IYAS GR +DAA+VRK MKESGVKKEPACSWVEIEN +H+FVAND+ HP REEI R WE+
Sbjct: 473 IYASGGRWNDAARVRKKMKESGVKKEPACSWVEIENAIHMFVANDERHPQREEIARKWEE 532

Query: 510 ISGKIKEIGYVPDTSHVLFFMNQQDRELKLQYHSEKLALAFAVLKTPPGLTIRIKKNIRI 569
           +  KIKE+GYVPDTSHV+  ++QQ+RE+ LQYHSEK+ALAFA+L TPPG TI IKKNIR+
Sbjct: 533 VLAKIKELGYVPDTSHVIVHVDQQEREVNLQYHSEKIALAFALLNTPPGSTIHIKKNIRV 592

Query: 570 CGDCHSAFKFASRVLGREIIVRDTNRFHHF 599
           CGDCH+A K AS+V+GREIIVRDTNRFHHF
Sbjct: 593 CGDCHTAIKLASKVVGREIIVRDTNRFHHF 622

BLAST of Cucsa.319460 vs. TAIR10
Match: AT3G13770.1 (AT3G13770.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 498.4 bits (1282), Expect = 6.1e-141
Identity = 262/630 (41.59%), Postives = 395/630 (62.70%), Query Frame = 1

Query: 2   DLLRTTHISSSGTSSN-------------STGLYVLDLINCGSLEPERTL--YSKMLNKC 61
           +L+R  H S S + +N             S G     L+    L PE     Y  +LN C
Sbjct: 3   NLMRLIHRSFSSSPTNYVLQTILPISQLCSNGRLQEALLEMAMLGPEMGFHGYDALLNAC 62

Query: 62  TYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWT 121
              R L+ G+ +HAH+  + +     L   +L  Y KC  LE+A+ + D+MP K++VSWT
Sbjct: 63  LDKRALRDGQRVHAHMIKTRYLPATYLRTRLLIFYGKCDCLEDARKVLDEMPEKNVVSWT 122

Query: 122 VLISGYSQSGQASEALALFPKMLHLGFQPNEFT----LSSLLKASGTGPSDHHGRQLHAF 181
            +IS YSQ+G +SEAL +F +M+    +PNEFT    L+S ++ASG G     G+Q+H  
Sbjct: 123 AMISRYSQTGHSSEALTVFAEMMRSDGKPNEFTFATVLTSCIRASGLGL----GKQIHGL 182

Query: 182 SLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHV 241
            +K+ YD ++ VGSSLLDMYA+   ++EA+ IF  L  ++VVS  A+IAG+A+ G  E  
Sbjct: 183 IVKWNYDSHIFVGSSLLDMYAKAGQIKEAREIFECLPERDVVSCTAIIAGYAQLGLDEEA 242

Query: 242 MRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDM 301
           + +F ++  +G  P + TY+S+ TA +    L+ GK  H HV++      A + N+LIDM
Sbjct: 243 LEMFHRLHSEGMSPNYVTYASLLTALSGLALLDHGKQAHCHVLRRELPFYAVLQNSLIDM 302

Query: 302 YAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAK-VQPNEIT 361
           Y+K G++  A+++F  + ++  +SWN+++ GY++HGLG E L+LF  M   K V+P+ +T
Sbjct: 303 YSKCGNLSYARRLFDNMPERTAISWNAMLVGYSKHGLGREVLELFRLMRDEKRVKPDAVT 362

Query: 362 FLSVLTACSHSGLLDEGQYYFELMK--KHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEE 421
            L+VL+ CSH  + D G   F+ M   ++  +    H+  +VD+LGRAGR++EA +FI+ 
Sbjct: 363 LLAVLSGCSHGRMEDTGLNIFDGMVAGEYGTKPGTEHYGCIVDMLGRAGRIDEAFEFIKR 422

Query: 422 MPIKPTAAVWGALLGACRMHKNMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDA 481
           MP KPTA V G+LLGACR+H ++D+G     ++ E++P ++G +V+LSN+YASAGR +D 
Sbjct: 423 MPSKPTAGVLGSLLGACRVHLSVDIGESVGRRLIEIEPENAGNYVILSNLYASAGRWADV 482

Query: 482 AKVRKMMKESGVKKEPACSWVEIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYV 541
             VR MM +  V KEP  SW++ E  +H F AND +HP REE+    ++IS K+K+ GYV
Sbjct: 483 NNVRAMMMQKAVTKEPGRSWIQHEQTLHYFHANDRTHPRREEVLAKMKEISIKMKQAGYV 542

Query: 542 PDTSHVLFFMNQQDRELKLQYHSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFA 601
           PD S VL+ ++++ +E  L  HSEKLAL F ++ T  G+ IR+ KN+RIC DCH+  K  
Sbjct: 543 PDLSCVLYDVDEEQKEKMLLGHSEKLALTFGLIATGEGIPIRVFKNLRICVDCHNFAKIF 602

Query: 602 SRVLGREIIVRDTNRFHHFLHGMCSCRDYW 610
           S+V  RE+ +RD NRFH  + G+CSC DYW
Sbjct: 603 SKVFEREVSLRDKNRFHQIVDGICSCGDYW 628

BLAST of Cucsa.319460 vs. TAIR10
Match: AT1G11290.1 (AT1G11290.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 491.5 bits (1264), Expect = 7.5e-139
Identity = 255/602 (42.36%), Postives = 366/602 (60.80%), Query Frame = 1

Query: 12  SGTSSNSTGLYVLDLINC---GSLEPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFE 71
           +G S N      L+++      +L+P       +L   + LR +  G+ IH +   S F+
Sbjct: 209 AGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFD 268

Query: 72  DDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKM 131
             + +   +++MYAKCGSLE A+ LFD M  +++VSW  +I  Y Q+    EA+ +F KM
Sbjct: 269 SLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKM 328

Query: 132 LHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHM 191
           L  G +P + ++   L A         GR +H  S++ G D NV V +SL+ MY +   +
Sbjct: 329 LDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEV 388

Query: 192 REAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTAC 251
             A  +F  L ++ +VSWNA+I G A+ G     +  F QM  +  +P  FTY SV TA 
Sbjct: 389 DTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAI 448

Query: 252 ASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWN 311
           A        KW+H  V++S      ++   L+DMYAK G+I  A+ +F  + ++ + +WN
Sbjct: 449 AELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWN 508

Query: 312 SIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKK- 371
           ++I GY  HG G  AL+LFE+M K  ++PN +TFLSV++ACSHSGL++ G   F +MK+ 
Sbjct: 509 AMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKEN 568

Query: 372 HKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVY 431
           + IE  + H+  +VDLLGRAGRLNEA  FI +MP+KP   V+GA+LGAC++HKN++    
Sbjct: 569 YSIELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEK 628

Query: 432 AAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWVEIENEVH 491
           AAE++FEL+P D G HVLL+NIY +A       +VR  M   G++K P CS VEI+NEVH
Sbjct: 629 AAERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGCSMVEIKNEVH 688

Query: 492 VFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQYHSEKLAL 551
            F +   +HP  ++I    EK+   IKE GYVPDT+ VL   N    +L L  HSEKLA+
Sbjct: 689 SFFSGSTAHPDSKKIYAFLEKLICHIKEAGYVPDTNLVLGVENDVKEQL-LSTHSEKLAI 748

Query: 552 AFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLHGMCSCRD 610
           +F +L T  G TI ++KN+R+C DCH+A K+ S V GREI+VRD  RFHHF +G CSC D
Sbjct: 749 SFGLLNTTAGTTIHVRKNLRVCADCHNATKYISLVTGREIVVRDMQRFHHFKNGACSCGD 808

HSP 2 Score: 227.3 bits (578), Expect = 2.6e-59
Identity = 132/442 (29.86%), Postives = 233/442 (52.71%), Query Frame = 1

Query: 32  LEPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQ 91
           +EP    ++ +L  C    +L+ G+ IH  +  S F  DL  +  + NMYAKC  + EA+
Sbjct: 131 VEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEAR 190

Query: 92  DLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGTGP 151
            +FD+MP +D+VSW  +++GYSQ+G A  AL +   M     +P+  T+ S+L A     
Sbjct: 191 KVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALR 250

Query: 152 SDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNALIA 211
               G+++H ++++ G+D  V++ ++L+DMYA+   +  A+ +F+ +  +NVVSWN++I 
Sbjct: 251 LISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMID 310

Query: 212 GHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQP 271
            + +    +  M +F +ML +G +PT  +      ACA  G LE+G+++H   ++ G   
Sbjct: 311 AYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDR 370

Query: 272 IAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQML 331
              + N+LI MY K   +  A  +F +L  + +VSWN++I G+AQ+G   +AL  F QM 
Sbjct: 371 NVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMR 430

Query: 332 KAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAGRLN 391
              V+P+  T++SV+TA +   +    ++   ++ +  ++  V     +VD+  + G + 
Sbjct: 431 SRTVKPDTFTYVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIM 490

Query: 392 EANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVYAAEQIFELDPHDSGPH--VLLSNI 451
            A + I +M  +     W A++     H     G  A E   E+      P+    LS I
Sbjct: 491 IA-RLIFDMMSERHVTTWNAMIDGYGTH---GFGKAALELFEEMQKGTIKPNGVTFLSVI 550

Query: 452 YA--SAGRLSDAAKVRKMMKES 470
            A   +G +    K   MMKE+
Sbjct: 551 SACSHSGLVEAGLKCFYMMKEN 568

HSP 3 Score: 178.3 bits (451), Expect = 1.4e-44
Identity = 93/376 (24.73%), Postives = 207/376 (55.05%), Query Frame = 1

Query: 42  MLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNF----ILNMYAKCGSLEEAQDLFDKM 101
           +L +C+ L++L+Q       I    F++ L   +F    +++++ + GS++EA  +F+ +
Sbjct: 43  LLERCSSLKELRQ-------ILPLVFKNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPI 102

Query: 102 PTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGR 161
            +K  V +  ++ G+++     +AL  F +M +   +P  +  + LLK  G       G+
Sbjct: 103 DSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGK 162

Query: 162 QLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKG 221
           ++H   +K G+ +++   + L +MYA+   + EA+ +F+ +  +++VSWN ++AG+++ G
Sbjct: 163 EIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNG 222

Query: 222 EGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGN 281
                + +   M  +  +P+  T  SV  A ++   +  GK +H + ++SG   +  I  
Sbjct: 223 MARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNIST 282

Query: 282 TLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQP 341
            L+DMYAK GS++ A+++F  ++++++VSWNS+I  Y Q+    EA+ +F++ML   V+P
Sbjct: 283 ALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKP 342

Query: 342 NEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFI 401
            +++ +  L AC+  G L+ G++  +L  +  ++  V+   +++ +  +   ++ A    
Sbjct: 343 TDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMF 402

Query: 402 EEMPIKPTAAVWGALL 414
            ++  + T   W A++
Sbjct: 403 GKLQSR-TLVSWNAMI 410

BLAST of Cucsa.319460 vs. TAIR10
Match: AT4G13650.1 (AT4G13650.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 479.6 bits (1233), Expect = 2.9e-135
Identity = 234/577 (40.55%), Postives = 363/577 (62.91%), Query Frame = 1

Query: 34   PERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDL 93
            P +  Y  +L  C  L  L+ G  IH+ I  + F+ +  + + +++MYAK G L+ A D+
Sbjct: 488  PNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTAWDI 547

Query: 94   FDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGTGPSD 153
              +   KD+VSWT +I+GY+Q     +AL  F +ML  G + +E  L++ + A     + 
Sbjct: 548  LIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAVSACAGLQAL 607

Query: 154  HHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNALIAGH 213
              G+Q+HA +   G+  ++   ++L+ +Y+R   + E+ + F    A + ++WNAL++G 
Sbjct: 608  KEGQQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEESYLAFEQTEAGDNIAWNALVSGF 667

Query: 214  ARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIA 273
             + G  E  +R+F++M R+G +  +FT+ S   A + + +++QGK VHA + K+G     
Sbjct: 668  QQSGNNEEALRVFVRMNREGIDNNNFTFGSAVKAASETANMKQGKQVHAVITKTGYDSET 727

Query: 274  YIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKA 333
             + N LI MYAK GSI DA+K F  +  ++ VSWN+II+ Y++HG G+EAL  F+QM+ +
Sbjct: 728  EVCNALISMYAKCGSISDAEKQFLEVSTKNEVSWNAIINAYSKHGFGSEALDSFDQMIHS 787

Query: 334  KVQPNEITFLSVLTACSHSGLLDEGQYYFELMK-KHKIEAQVAHHVTVVDLLGRAGRLNE 393
             V+PN +T + VL+ACSH GL+D+G  YFE M  ++ +  +  H+V VVD+L RAG L+ 
Sbjct: 788  NVRPNHVTLVGVLSACSHIGLVDKGIAYFESMNSEYGLSPKPEHYVCVVDMLTRAGLLSR 847

Query: 394  ANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVYAAEQIFELDPHDSGPHVLLSNIYAS 453
            A +FI+EMPIKP A VW  LL AC +HKNM++G +AA  + EL+P DS  +VLLSN+YA 
Sbjct: 848  AKEFIQEMPIKPDALVWRTLLSACVVHKNMEIGEFAAHHLLELEPEDSATYVLLSNLYAV 907

Query: 454  AGRLSDAAKVRKMMKESGVKKEPACSWVEIENEVHVFVANDDSHPMREEIQRMWEKISGK 513
            + +       R+ MKE GVKKEP  SW+E++N +H F   D +HP+ +EI   ++ ++ +
Sbjct: 908  SKKWDARDLTRQKMKEKGVKKEPGQSWIEVKNSIHSFYVGDQNHPLADEIHEYFQDLTKR 967

Query: 514  IKEIGYVPDTSHVLFFMNQQDRELKLQYHSEKLALAFAVLKTPPGLTIRIKKNIRICGDC 573
              EIGYV D   +L  +  + ++  +  HSEKLA++F +L  P  + I + KN+R+C DC
Sbjct: 968  ASEIGYVQDCFSLLNELQHEQKDPIIFIHSEKLAISFGLLSLPATVPINVMKNLRVCNDC 1027

Query: 574  HSAFKFASRVLGREIIVRDTNRFHHFLHGMCSCRDYW 610
            H+  KF S+V  REIIVRD  RFHHF  G CSC+DYW
Sbjct: 1028 HAWIKFVSKVSNREIIVRDAYRFHHFEGGACSCKDYW 1064

HSP 2 Score: 208.0 bits (528), Expect = 1.7e-53
Identity = 119/401 (29.68%), Postives = 202/401 (50.37%), Query Frame = 1

Query: 21  LYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNM 80
           +YVL ++      P    +S +L+ C  +  L+ G  +H  +    F  D  + N ++++
Sbjct: 279 MYVLGIM------PTPYAFSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNALVSL 338

Query: 81  YAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTL 140
           Y   G+L  A+ +F  M  +D V++  LI+G SQ G   +A+ LF +M   G +P+  TL
Sbjct: 339 YFHLGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPDSNTL 398

Query: 141 SSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAA 200
           +SL+ A     +   G+QLHA++ K G+  N  +  +LL++YA+ A +  A   F     
Sbjct: 399 ASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFLETEV 458

Query: 201 KNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWV 260
           +NVV WN ++  +    +  +  R+F QM  +   P  +TY S+   C   G LE G+ +
Sbjct: 459 ENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLGDLELGEQI 518

Query: 261 HAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLG 320
           H+ +IK+  Q  AY+ + LIDMYAK G +  A  +  R   +D+VSW ++I+GY Q+   
Sbjct: 519 HSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTAWDILIRFAGKDVVSWTTMIAGYTQYNFD 578

Query: 321 AEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTV 380
            +AL  F QML   ++ +E+   + ++AC+    L EGQ            + +     +
Sbjct: 579 DKALTTFRQMLDRGIRSDEVGLTNAVSACAGLQALKEGQQIHAQACVSGFSSDLPFQNAL 638

Query: 381 VDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHKN 422
           V L  R G++ E+    E+       A W AL+   +   N
Sbjct: 639 VTLYSRCGKIEESYLAFEQTEAGDNIA-WNALVSGFQQSGN 672

HSP 3 Score: 181.8 bits (460), Expect = 1.3e-45
Identity = 114/449 (25.39%), Postives = 214/449 (47.66%), Query Frame = 1

Query: 32  LEPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQ 91
           LEP+    + ++  C+    L +G+ +HA+     F  +  +   +LN+YAKC  +E A 
Sbjct: 385 LEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETAL 444

Query: 92  DLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGTGP 151
           D F +   +++V W V++  Y        +  +F +M      PN++T  S+LK      
Sbjct: 445 DYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLG 504

Query: 152 SDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNALIA 211
               G Q+H+  +K  + +N +V S L+DMYA+   +  A  I    A K+VVSW  +IA
Sbjct: 505 DLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTAWDILIRFAGKDVVSWTTMIA 564

Query: 212 GHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQP 271
           G+ +    +  +  F QML +G        ++  +ACA   +L++G+ +HA    SG   
Sbjct: 565 GYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAVSACAGLQALKEGQQIHAQACVSGFSS 624

Query: 272 IAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQML 331
                N L+ +Y++ G I+++   F +    D ++WN+++SG+ Q G   EAL++F +M 
Sbjct: 625 DLPFQNALVTLYSRCGKIEESYLAFEQTEAGDNIAWNALVSGFQQSGNNEEALRVFVRMN 684

Query: 332 KAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAGRLN 391
           +  +  N  TF S + A S +  + +G+    ++ K   +++      ++ +  + G ++
Sbjct: 685 REGIDNNNFTFGSAVKAASETANMKQGKQVHAVITKTGYDSETEVCNALISMYAKCGSIS 744

Query: 392 EANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVYAAEQIFELDPHDSGP-HVLLSNIY 451
           +A K   E+  K   + W A++ A   H     G  A +   ++   +  P HV L  + 
Sbjct: 745 DAEKQFLEVSTKNEVS-WNAIINAYSKH---GFGSEALDSFDQMIHSNVRPNHVTLVGVL 804

Query: 452 ASAGRL----SDAAKVRKMMKESGVKKEP 476
           ++   +       A    M  E G+  +P
Sbjct: 805 SACSHIGLVDKGIAYFESMNSEYGLSPKP 829

HSP 4 Score: 174.5 bits (441), Expect = 2.0e-43
Identity = 101/336 (30.06%), Postives = 177/336 (52.68%), Query Frame = 1

Query: 58  IHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQ 117
           IHA I      D  V+ N ++++Y++ G ++ A+ +FD +  KD  SW  +ISG S++  
Sbjct: 209 IHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDGLRLKDHSSWVAMISGLSKNEC 268

Query: 118 ASEALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSS 177
            +EA+ LF  M  LG  P  +  SS+L A     S   G QLH   LK G+  + +V ++
Sbjct: 269 EAEAIRLFCDMYVLGIMPTPYAFSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNA 328

Query: 178 LLDMYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPT 237
           L+ +Y    ++  A+ IF++++ ++ V++N LI G ++ G GE  M LF +M   G EP 
Sbjct: 329 LVSLYFHLGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPD 388

Query: 238 HFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFR 297
             T +S+  AC++ G+L +G+ +HA+  K G      I   L+++YAK   I+ A   F 
Sbjct: 389 SNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFL 448

Query: 298 RLVKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDE 357
               +++V WN ++  Y        + ++F QM   ++ PN+ T+ S+L  C   G L+ 
Sbjct: 449 ETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLGDLEL 508

Query: 358 GQYYFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEA 394
           G+     + K   +        ++D+  + G+L+ A
Sbjct: 509 GEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTA 544

HSP 5 Score: 163.3 bits (412), Expect = 4.7e-40
Identity = 111/419 (26.49%), Postives = 200/419 (47.73%), Query Frame = 1

Query: 24  LDLINCGSLEPERTLYSKMLNKCTYLR-KLKQGRAIHAHIQSSTFEDDLVLLNFILNMYA 83
           +D +    + P       +L  C      L +GR +H+ I     + +  L   + + Y 
Sbjct: 72  IDSVENRGIRPNHQTLKWLLEGCLKTNGSLDEGRKLHSQILKLGLDSNGCLSEKLFDFYL 131

Query: 84  KCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSS 143
             G L  A  +FD+MP + + +W  +I   +      E   LF +M+     PNE T S 
Sbjct: 132 FKGDLYGAFKVFDEMPERTIFTWNKMIKELASRNLIGEVFGLFVRMVSENVTPNEGTFSG 191

Query: 144 LLKAS-GTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAK 203
           +L+A  G   +     Q+HA  L  G   +  V + L+D+Y+R   +  A+ +F+ L  K
Sbjct: 192 VLEACRGGSVAFDVVEQIHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDGLRLK 251

Query: 204 NVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVH 263
           +  SW A+I+G ++       +RLF  M   G  PT + +SSV +AC    SLE G+ +H
Sbjct: 252 DHSSWVAMISGLSKNECEAEAIRLFCDMYVLGIMPTPYAFSSVLSACKKIESLEIGEQLH 311

Query: 264 AHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGA 323
             V+K G     Y+ N L+ +Y   G++  A+ +F  + ++D V++N++I+G +Q G G 
Sbjct: 312 GLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGE 371

Query: 324 EALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVV 383
           +A++LF++M    ++P+  T  S++ ACS  G L  GQ       K    +       ++
Sbjct: 372 KAMELFKRMHLDGLEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALL 431

Query: 384 DLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHKNM--DLGVYAAEQIFELDPH 439
           +L  +   +  A  +  E  ++    +W  +L A  +  ++     ++   QI E+ P+
Sbjct: 432 NLYAKCADIETALDYFLETEVE-NVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPN 489

BLAST of Cucsa.319460 vs. TAIR10
Match: AT3G12770.1 (AT3G12770.1 mitochondrial editing factor 22)

HSP 1 Score: 478.8 bits (1231), Expect = 5.0e-135
Identity = 231/580 (39.83%), Postives = 366/580 (63.10%), Query Frame = 1

Query: 32  LEPERTLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQ 91
           + P+   +  +L  C+ L  L+ GR +HA +    F+ D+ + N ++ +YAKC  L  A+
Sbjct: 115 VSPDSFTFPHLLKACSGLSHLQMGRFVHAQVFRLGFDADVFVQNGLIALYAKCRRLGSAR 174

Query: 92  DLFD--KMPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGT 151
            +F+   +P + +VSWT ++S Y+Q+G+  EAL +F +M  +  +P+   L S+L A   
Sbjct: 175 TVFEGLPLPERTIVSWTAIVSAYAQNGEPMEALEIFSQMRKMDVKPDWVALVSVLNAFTC 234

Query: 152 GPSDHHGRQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNSLAAKNVVSWNAL 211
                 GR +HA  +K G ++   +  SL  MYA+   +  AK++F+ + + N++ WNA+
Sbjct: 235 LQDLKQGRSIHASVVKMGLEIEPDLLISLNTMYAKCGQVATAKILFDKMKSPNLILWNAM 294

Query: 212 IAGHARKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGG 271
           I+G+A+ G     + +F +M+ +   P   + +S  +ACA  GSLEQ + ++ +V +S  
Sbjct: 295 ISGYAKNGYAREAIDMFHEMINKDVRPDTISITSAISACAQVGSLEQARSMYEYVGRSDY 354

Query: 272 QPIAYIGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQ 331
           +   +I + LIDM+AK GS++ A+ VF R + +D+V W+++I GY  HG   EA+ L+  
Sbjct: 355 RDDVFISSALIDMFAKCGSVEGARLVFDRTLDRDVVVWSAMIVGYGLHGRAREAISLYRA 414

Query: 332 MLKAKVQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAGR 391
           M +  V PN++TFL +L AC+HSG++ EG ++F  M  HKI  Q  H+  V+DLLGRAG 
Sbjct: 415 MERGGVHPNDVTFLGLLMACNHSGMVREGWWFFNRMADHKINPQQQHYACVIDLLGRAGH 474

Query: 392 LNEANKFIEEMPIKPTAAVWGALLGACRMHKNMDLGVYAAEQIFELDPHDSGPHVLLSNI 451
           L++A + I+ MP++P   VWGALL AC+ H++++LG YAA+Q+F +DP ++G +V LSN+
Sbjct: 475 LDQAYEVIKCMPVQPGVTVWGALLSACKKHRHVELGEYAAQQLFSIDPSNTGHYVQLSNL 534

Query: 452 YASAGRLSDAAKVRKMMKESGVKKEPACSWVEIENEVHVFVANDDSHPMREEIQRMWEKI 511
           YA+A      A+VR  MKE G+ K+  CSWVE+   +  F   D SHP  EEI+R  E I
Sbjct: 535 YAAARLWDRVAEVRVRMKEKGLNKDVGCSWVEVRGRLEAFRVGDKSHPRYEEIERQVEWI 594

Query: 512 SGKIKEIGYVPDTSHVLFFMNQQDRELKLQYHSEKLALAFAVLKTPPGLTIRIKKNIRIC 571
             ++KE G+V +    L  +N ++ E  L  HSE++A+A+ ++ TP G  +RI KN+R C
Sbjct: 595 ESRLKEGGFVANKDASLHDLNDEEAEETLCSHSERIAIAYGLISTPQGTPLRITKNLRAC 654

Query: 572 GDCHSAFKFASRVLGREIIVRDTNRFHHFLHGMCSCRDYW 610
            +CH+A K  S+++ REI+VRDTNRFHHF  G+CSC DYW
Sbjct: 655 VNCHAATKLISKLVDREIVVRDTNRFHHFKDGVCSCGDYW 694

HSP 2 Score: 187.2 bits (474), Expect = 3.0e-47
Identity = 107/407 (26.29%), Postives = 206/407 (50.61%), Query Frame = 1

Query: 37  TLYSKMLNKCTYLRKLKQGRAIHAHIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDK 96
           + Y+ +++  T+  +LKQ   IHA +     +    L+  +++  +  G +  A+ +FD 
Sbjct: 22  SFYASLIDSATHKAQLKQ---IHARLLVLGLQFSGFLITKLIHASSSFGDITFARQVFDD 81

Query: 97  MPTKDMVSWTVLISGYSQSGQASEALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHG 156
           +P   +  W  +I GYS++    +AL ++  M      P+ FT   LLKA         G
Sbjct: 82  LPRPQIFPWNAIIRGYSRNNHFQDALLMYSNMQLARVSPDSFTFPHLLKACSGLSHLQMG 141

Query: 157 RQLHAFSLKYGYDMNVHVGSSLLDMYARWAHMREAKVIFNS--LAAKNVVSWNALIAGHA 216
           R +HA   + G+D +V V + L+ +YA+   +  A+ +F    L  + +VSW A+++ +A
Sbjct: 142 RFVHAQVFRLGFDADVFVQNGLIALYAKCRRLGSARTVFEGLPLPERTIVSWTAIVSAYA 201

Query: 217 RKGEGEHVMRLFLQMLRQGFEPTHFTYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAY 276
           + GE    + +F QM +   +P      SV  A      L+QG+ +HA V+K G +    
Sbjct: 202 QNGEPMEALEIFSQMRKMDVKPDWVALVSVLNAFTCLQDLKQGRSIHASVVKMGLEIEPD 261

Query: 277 IGNTLIDMYAKSGSIKDAKKVFRRLVKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAK 336
           +  +L  MYAK G +  AK +F ++   +++ WN++ISGYA++G   EA+ +F +M+   
Sbjct: 262 LLISLNTMYAKCGQVATAKILFDKMKSPNLILWNAMISGYAKNGYAREAIDMFHEMINKD 321

Query: 337 VQPNEITFLSVLTACSHSGLLDEGQYYFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEAN 396
           V+P+ I+  S ++AC+  G L++ +  +E + +      V     ++D+  + G + E  
Sbjct: 322 VRPDTISITSAISACAQVGSLEQARSMYEYVGRSDYRDDVFISSALIDMFAKCGSV-EGA 381

Query: 397 KFIEEMPIKPTAAVWGALLGACRMH--KNMDLGVYAAEQIFELDPHD 440
           + + +  +     VW A++    +H      + +Y A +   + P+D
Sbjct: 382 RLVFDRTLDRDVVVWSAMIVGYGLHGRAREAISLYRAMERGGVHPND 424

BLAST of Cucsa.319460 vs. NCBI nr
Match: gi|449443492|ref|XP_004139511.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g24000, mitochondrial-like [Cucumis sativus])

HSP 1 Score: 1231.1 bits (3184), Expect = 0.0e+00
Identity = 608/609 (99.84%), Postives = 609/609 (100.00%), Query Frame = 1

Query: 1   EDLLRTTHISSSGTSSNSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA 60
           EDLLRTTHISSSGTSSNSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA
Sbjct: 70  EDLLRTTHISSSGTSSNSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA 129

Query: 61  HIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASE 120
           HIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASE
Sbjct: 130 HIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASE 189

Query: 121 ALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLD 180
           ALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLD
Sbjct: 190 ALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLD 249

Query: 181 MYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFT 240
           MYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFT
Sbjct: 250 MYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFT 309

Query: 241 YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLV 300
           YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLV
Sbjct: 310 YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLV 369

Query: 301 KQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQY 360
           KQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQY
Sbjct: 370 KQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQY 429

Query: 361 YFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHK 420
           YFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLG+CRMHK
Sbjct: 430 YFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGSCRMHK 489

Query: 421 NMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWV 480
           NMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWV
Sbjct: 490 NMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWV 549

Query: 481 EIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQY 540
           EIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQY
Sbjct: 550 EIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQY 609

Query: 541 HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLH 600
           HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLH
Sbjct: 610 HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLH 669

Query: 601 GMCSCRDYW 610
           GMCSCRDYW
Sbjct: 670 GMCSCRDYW 678

BLAST of Cucsa.319460 vs. NCBI nr
Match: gi|659128608|ref|XP_008464284.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g24000, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 579/609 (95.07%), Postives = 593/609 (97.37%), Query Frame = 1

Query: 1   EDLLRTTHISSSGTSSNSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA 60
           EDLLRTTHISSS  SS+STGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA
Sbjct: 72  EDLLRTTHISSSDVSSSSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA 131

Query: 61  HIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASE 120
           HIQSS FE+D VLLNFILNMYAKCGSLEEAQDLFDKMPTKD VSWTVLISGYSQS +ASE
Sbjct: 132 HIQSSAFENDPVLLNFILNMYAKCGSLEEAQDLFDKMPTKDRVSWTVLISGYSQSRRASE 191

Query: 121 ALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLD 180
           ALALFPKMLHLGFQPNEFTLSSLLKASG GPSD HGRQLHAFSLKYGYDMNVHVGSSLLD
Sbjct: 192 ALALFPKMLHLGFQPNEFTLSSLLKASGAGPSDDHGRQLHAFSLKYGYDMNVHVGSSLLD 251

Query: 181 MYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFT 240
           MYARW HMREAKVIF SLAAKNVVSWNALIAGHARKGEGEHVMRLF QMLRQGFEPTHFT
Sbjct: 252 MYARWGHMREAKVIFKSLAAKNVVSWNALIAGHARKGEGEHVMRLFSQMLRQGFEPTHFT 311

Query: 241 YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLV 300
           YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVF+RLV
Sbjct: 312 YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFQRLV 371

Query: 301 KQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQY 360
           K+DIVSWNSIISGYAQHGLGAEALQLFEQ+LKAKVQPNEITFLSVLTACSHSGLLDEG+Y
Sbjct: 372 KRDIVSWNSIISGYAQHGLGAEALQLFEQVLKAKVQPNEITFLSVLTACSHSGLLDEGKY 431

Query: 361 YFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHK 420
           YFELMKKH IE QVAHHVTVVDLLGRAGRLNEANKFIEEMP++PTAAVWGALLGACRMHK
Sbjct: 432 YFELMKKHGIEPQVAHHVTVVDLLGRAGRLNEANKFIEEMPMEPTAAVWGALLGACRMHK 491

Query: 421 NMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWV 480
           NMDLGVYAAE+IFELDPHDSGPHVLLSNIYASAGRL DA  VRKMMKESGVKKEPACSWV
Sbjct: 492 NMDLGVYAAEKIFELDPHDSGPHVLLSNIYASAGRLRDAGNVRKMMKESGVKKEPACSWV 551

Query: 481 EIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQY 540
           EIENEVH+FVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFM+QQDRE+KLQY
Sbjct: 552 EIENEVHMFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMDQQDREVKLQY 611

Query: 541 HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLH 600
           HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFAS+VLGREIIVRDTNRFHHFLH
Sbjct: 612 HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASKVLGREIIVRDTNRFHHFLH 671

Query: 601 GMCSCRDYW 610
           GMCSCRDYW
Sbjct: 672 GMCSCRDYW 680

BLAST of Cucsa.319460 vs. NCBI nr
Match: gi|659128610|ref|XP_008464285.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g24000, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 579/609 (95.07%), Postives = 593/609 (97.37%), Query Frame = 1

Query: 1   EDLLRTTHISSSGTSSNSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA 60
           EDLLRTTHISSS  SS+STGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA
Sbjct: 36  EDLLRTTHISSSDVSSSSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA 95

Query: 61  HIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASE 120
           HIQSS FE+D VLLNFILNMYAKCGSLEEAQDLFDKMPTKD VSWTVLISGYSQS +ASE
Sbjct: 96  HIQSSAFENDPVLLNFILNMYAKCGSLEEAQDLFDKMPTKDRVSWTVLISGYSQSRRASE 155

Query: 121 ALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLD 180
           ALALFPKMLHLGFQPNEFTLSSLLKASG GPSD HGRQLHAFSLKYGYDMNVHVGSSLLD
Sbjct: 156 ALALFPKMLHLGFQPNEFTLSSLLKASGAGPSDDHGRQLHAFSLKYGYDMNVHVGSSLLD 215

Query: 181 MYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFT 240
           MYARW HMREAKVIF SLAAKNVVSWNALIAGHARKGEGEHVMRLF QMLRQGFEPTHFT
Sbjct: 216 MYARWGHMREAKVIFKSLAAKNVVSWNALIAGHARKGEGEHVMRLFSQMLRQGFEPTHFT 275

Query: 241 YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLV 300
           YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVF+RLV
Sbjct: 276 YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFQRLV 335

Query: 301 KQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQY 360
           K+DIVSWNSIISGYAQHGLGAEALQLFEQ+LKAKVQPNEITFLSVLTACSHSGLLDEG+Y
Sbjct: 336 KRDIVSWNSIISGYAQHGLGAEALQLFEQVLKAKVQPNEITFLSVLTACSHSGLLDEGKY 395

Query: 361 YFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHK 420
           YFELMKKH IE QVAHHVTVVDLLGRAGRLNEANKFIEEMP++PTAAVWGALLGACRMHK
Sbjct: 396 YFELMKKHGIEPQVAHHVTVVDLLGRAGRLNEANKFIEEMPMEPTAAVWGALLGACRMHK 455

Query: 421 NMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWV 480
           NMDLGVYAAE+IFELDPHDSGPHVLLSNIYASAGRL DA  VRKMMKESGVKKEPACSWV
Sbjct: 456 NMDLGVYAAEKIFELDPHDSGPHVLLSNIYASAGRLRDAGNVRKMMKESGVKKEPACSWV 515

Query: 481 EIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQY 540
           EIENEVH+FVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFM+QQDRE+KLQY
Sbjct: 516 EIENEVHMFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMDQQDREVKLQY 575

Query: 541 HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLH 600
           HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFAS+VLGREIIVRDTNRFHHFLH
Sbjct: 576 HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASKVLGREIIVRDTNRFHHFLH 635

Query: 601 GMCSCRDYW 610
           GMCSCRDYW
Sbjct: 636 GMCSCRDYW 644

BLAST of Cucsa.319460 vs. NCBI nr
Match: gi|645236270|ref|XP_008224655.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g24000, mitochondrial-like [Prunus mume])

HSP 1 Score: 923.3 bits (2385), Expect = 2.2e-265
Identity = 440/611 (72.01%), Postives = 530/611 (86.74%), Query Frame = 1

Query: 1   EDLLRTTHISSSGTSSNSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA 60
           +DLLR + + +SGT     GL+VLDL+N GSLE +RTLY+K+LN+CT + +L+Q R +HA
Sbjct: 64  KDLLRKS-LHNSGT-----GLHVLDLVNRGSLEADRTLYNKLLNRCTQMGRLQQARIVHA 123

Query: 61  HIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASE 120
           HI +S F+DDL + N ILNMY KCGSLE+A++LFD+MP KD+V+WT LISGYSQ+ +  +
Sbjct: 124 HILTSHFKDDLPIHNTILNMYVKCGSLEDARNLFDQMPCKDLVTWTALISGYSQNDRPQD 183

Query: 121 ALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDH--HGRQLHAFSLKYGYDMNVHVGSSL 180
           AL LFP+ML  G +PN+FTLSSL KA+G    D+  HGRQLH + LKYG+D NV+VG+SL
Sbjct: 184 ALVLFPQMLLRGLEPNQFTLSSLFKAAGAVSDDNNEHGRQLHTYCLKYGFDTNVYVGTSL 243

Query: 181 LDMYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTH 240
           +DMYARW HM E+++IF+SL  KN VSWNALIAGHARK +GEH +RL  +MLR+GF+PTH
Sbjct: 244 VDMYARWGHMDESQLIFDSLETKNEVSWNALIAGHARKAQGEHALRLLGKMLREGFKPTH 303

Query: 241 FTYSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRR 300
           FTYSSVF ACAS+GS+EQGKWVHAH+IKSG + +A++GNTL+DMYAKSGSI+DA+KVF R
Sbjct: 304 FTYSSVFIACASAGSMEQGKWVHAHMIKSGAKLVAFVGNTLLDMYAKSGSIEDARKVFDR 363

Query: 301 LVKQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEG 360
           LV+QDIVSWNS+++GYAQHGLG E +Q FE+ML+  +QPN+ITFL VLTACSH+GLLDEG
Sbjct: 364 LVRQDIVSWNSMLTGYAQHGLGQETVQRFEEMLRIGIQPNDITFLCVLTACSHAGLLDEG 423

Query: 361 QYYFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRM 420
           QYYF+LMK + IE Q++H+VT+VDLLGRAG L+ A KFI EMPI+PTAAVWGALLGACRM
Sbjct: 424 QYYFDLMKSYNIEPQISHYVTIVDLLGRAGLLDRAEKFIREMPIEPTAAVWGALLGACRM 483

Query: 421 HKNMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACS 480
           HKN+DLG YAAE++FELDPHDSGPHVLLSNIYASAGRLSDAA+VRK+MK+ GVKKEPACS
Sbjct: 484 HKNIDLGAYAAERVFELDPHDSGPHVLLSNIYASAGRLSDAARVRKLMKDCGVKKEPACS 543

Query: 481 WVEIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKL 540
           WVEIEN VH+FVANDD+HP R EI +MWE ISGKIKEIGYVPDTSHVLFF++QQ+RE+KL
Sbjct: 544 WVEIENAVHMFVANDDAHPQRVEILQMWETISGKIKEIGYVPDTSHVLFFVDQQEREVKL 603

Query: 541 QYHSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHF 600
           QYHSEKLALAFA+L T PG TIRIKKNIR+CGDCHSA K+ S+V GREIIVRDTNRFHHF
Sbjct: 604 QYHSEKLALAFALLNTTPGSTIRIKKNIRVCGDCHSAIKYVSKVEGREIIVRDTNRFHHF 663

Query: 601 LHGMCSCRDYW 610
             G CSCRDYW
Sbjct: 664 RDGSCSCRDYW 668

BLAST of Cucsa.319460 vs. NCBI nr
Match: gi|1009111377|ref|XP_015900763.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g24000, mitochondrial-like [Ziziphus jujuba])

HSP 1 Score: 911.8 bits (2355), Expect = 6.6e-262
Identity = 433/609 (71.10%), Postives = 520/609 (85.39%), Query Frame = 1

Query: 1   EDLLRTTHISSSGTSSNSTGLYVLDLINCGSLEPERTLYSKMLNKCTYLRKLKQGRAIHA 60
           +DLLR ++          TGL+VLDLI+ GSLE +R LY+ +L +CT + KL++GR +HA
Sbjct: 66  KDLLRKSY------GGPGTGLHVLDLIDRGSLEADRILYNTLLKRCTQMGKLREGRIVHA 125

Query: 61  HIQSSTFEDDLVLLNFILNMYAKCGSLEEAQDLFDKMPTKDMVSWTVLISGYSQSGQASE 120
           HI +S F+DDLV+ N ILNMYAKCG L++A+ LFD+MP KDMV+WT LI+GYSQ+ Q  +
Sbjct: 126 HILNSQFKDDLVMGNTILNMYAKCGGLKDARTLFDEMPVKDMVTWTALITGYSQNDQPED 185

Query: 121 ALALFPKMLHLGFQPNEFTLSSLLKASGTGPSDHHGRQLHAFSLKYGYDMNVHVGSSLLD 180
           AL LFP+ML  G +PN+FTLSSLLKASG  P+D +GRQLHA+ LKYG+  NV+VGSSL+D
Sbjct: 186 ALVLFPQMLRHGLEPNQFTLSSLLKASGGRPTDKYGRQLHAYCLKYGFVSNVYVGSSLVD 245

Query: 181 MYARWAHMREAKVIFNSLAAKNVVSWNALIAGHARKGEGEHVMRLFLQMLRQGFEPTHFT 240
           MYAR  HM EA+ IF+ + +KN VSWNALIAGHARKGEGEH  RLF +M R+ F+PTHFT
Sbjct: 246 MYARCGHMVEAQSIFDDIVSKNEVSWNALIAGHARKGEGEHAFRLFWKMQREDFKPTHFT 305

Query: 241 YSSVFTACASSGSLEQGKWVHAHVIKSGGQPIAYIGNTLIDMYAKSGSIKDAKKVFRRLV 300
           YSS+  ACAS+GSLEQGKWVHAH+IKSGG+ +A+ GNTL+ MYAKSGSI+DAKKVF RL+
Sbjct: 306 YSSILAACASTGSLEQGKWVHAHMIKSGGRLVAFAGNTLLYMYAKSGSIEDAKKVFDRLL 365

Query: 301 KQDIVSWNSIISGYAQHGLGAEALQLFEQMLKAKVQPNEITFLSVLTACSHSGLLDEGQY 360
           KQD+VSWNSI++GYAQHGL  E LQ FE+ML+  ++PN+ITFL VLTACSH+GLLDEGQY
Sbjct: 366 KQDLVSWNSILTGYAQHGLAKETLQQFEEMLRIGIEPNDITFLCVLTACSHAGLLDEGQY 425

Query: 361 YFELMKKHKIEAQVAHHVTVVDLLGRAGRLNEANKFIEEMPIKPTAAVWGALLGACRMHK 420
           Y ELMKK+K+E QV+H+VT+VDLLGRAG L+ A +FI+EMPI+PTAAVWGALLGACRMHK
Sbjct: 426 YLELMKKYKVEPQVSHYVTIVDLLGRAGLLDRAERFIKEMPIEPTAAVWGALLGACRMHK 485

Query: 421 NMDLGVYAAEQIFELDPHDSGPHVLLSNIYASAGRLSDAAKVRKMMKESGVKKEPACSWV 480
           NM+LG YAAE++FELDPHDSGPH+LL NIYASAGR  DAAKVRKMMK+SGVKKEPACSWV
Sbjct: 486 NMELGAYAAERVFELDPHDSGPHILLYNIYASAGRWDDAAKVRKMMKDSGVKKEPACSWV 545

Query: 481 EIENEVHVFVANDDSHPMREEIQRMWEKISGKIKEIGYVPDTSHVLFFMNQQDRELKLQY 540
           EIEN VH+FVANDD+HP REEI RMWE I  KIK+IGY+PDTSHVL F++QQ+RELKLQY
Sbjct: 546 EIENAVHMFVANDDAHPQREEIHRMWENIQSKIKKIGYIPDTSHVLLFVDQQERELKLQY 605

Query: 541 HSEKLALAFAVLKTPPGLTIRIKKNIRICGDCHSAFKFASRVLGREIIVRDTNRFHHFLH 600
           HSEKLALAF++L TPPG +IRIKKNIR+CGDCHSA K+ S+V+GREII+RDTNRFHHF  
Sbjct: 606 HSEKLALAFSLLNTPPGSSIRIKKNIRVCGDCHSAIKYVSKVVGREIILRDTNRFHHFRD 665

Query: 601 GMCSCRDYW 610
           G CSC DYW
Sbjct: 666 GSCSCGDYW 668