Cucsa.257480 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.257480
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPectinesterase
Locationscaffold02229 : 4748879 .. 4753641 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAGACACACGATCGTGTTTTTCGTTGTTCTTATTGTTCTTATTTTTCCGTCAGACATTTTATGTTATTCGGAAAAAGAAATAAAAAATTGGTGTAGCCAAACACCATATCCAGCACCATGTGAAGAATTTCTCAAAACCAAAGCTACTACAAAGAAAACTCCAATAACCACAAAATCCCATTTCTTTGAAATCTTAGTCGAAACGGCACTAGAGCGTGCCGTTTCAGCCCATAAAAATGCTCTTTCTCTCGGCCCAAAATGCCGAAATTCCAAAGAAAAAACTGCATGGACCGATTGCGTTGACCTCTACGACCAAATCATAACCCGACTCAACCGAACCTCTGCCCGCTGCTCACCAGCCGACGCTCAAACCTGGCTCAGCGCCGCTCTCACCGCCCTCGAAACCTGCCGGACAGGGTTCGAAGAACTCGGCCTTTCGGCTTTCGGCTACCCATTGACGGCTAATAATGTTTCCAAGCTGATTAGTGATGGGTTGTCGGTGAATAAACCCGCATCACCGGAGGGGTATGAGCCGACGACCATGACTGATGGGTTTCCCACGTGGGTTAGTCCTGGTAACCGTAAATTGCTGCAATCTGAGTCTCCGAAGGCGGACGTAGTGGTGGCGCAAGATGGGTCGGGGAATTTTAAGACGGTGAAGGATGCTATCTCCGCCGCAAAGGGCGGCGGGAGATTTGTGATATATATAAAGTCGGGGGTTTATAATGAGAATTTGGATATTAAGGCTAAGAATGTTATGATGGTCGGCGACGGAATCGGAAAAACTATCATTACCGGCAGTAGAAGTGTCGGCGGAGGTTCCACCACGTTCAGATCCGCTACCGTTGGTAAGCCCTCTCTCTTTTTCTCTTTAAAACTTTCTTTTTCCTTCAAACTTTTCATTTTTTATTTTCATAAAGTTATTAATTTAAATGGGCCCTTCCAAATATGAGCTCTACATATTACAAATTAGAGAATTTAAGTTAAGATTTAAAAAGCTTTAATTAATATTTGAACTTTAATATGATAACATTAAATAGTGGATAATGGTTGATGTGTGTTGCTAAACCTATTCTTTTTTCGAAAGATGAAAAGTAAAATTCACGAATTCTATTTAACCATATAGATTAGTGGAACAACGAGTTGTTTTTGTCAAAATAGAAGTTTGTAGGAGTAGAAAAGTGAAAAAAGGATATTTTTTTAACTCTATAGTTAACTATCAAAATTTTAACATTAATCAATAAGAATTAGCTAAGACTTATGAAAGACATAAAATTTAACTTCAAATCAAACATTAATTTTTTAAATAATCTACTTTGGCAACTTCCCCTTAATTAAATAGGATTAGAGTGATAATACGAGGTCTTGAAATTTATTATAAACTTAGTTGTTGTCATTGTATTTATTATAAACTTGCTTGTAGTGCATGGCGCTACCATCATCTCATATGTCACGATGTGTCGGGAAAGTCATTGATTTAGGGAAGATAAAAACACAATAATATTAAATTTTATGTAATATATTATTGGATTTTAAAAAAATCAAAATAACATTAGAAAAAGGTTAAATGTACCTAATACATATGATAATGTCATGTAAGTTGACTTTATATATAAACTAATCTATGGATGAAAATTCACAATGTCACATTATGAATTTTTAGTTTCAATTTAATATTAATCGGCGACCAATAGGAAATCAACAAACCTACTTTGAGTAAATTAGTAAATTTTGCTGACTGATTTAATTGCTAATAGTAATTAATATAGTTTTGAATTGTTCTTCATTGCCAAACCCTATAATTCTATGTTTTATAACTTTCTTGAGAATTTATTCATTTCTATTGGTTGTAATTAATTTTGGGGTTGAATTATATGCACTTAAGTGACTTTTATTTCAATATATTTAGAAGTGTGAATCGTATGACCCTCTGTTTAATTAATTATAGTTGTAGTAATTAAAAATTGACGTGTGAAATAATTATACGGTAGTTTTAGTTTAAATTTTTAAGAAACCCTACAAAGTTATAGCTACAATTAGAGTAATACTTTTTAAGGTTTATATTTAATTCGGTTTCTAATTGAACTTCAAAAAGTGACTATTTTGGTCCTTTTCTTTTCATTTTCTTAGTACAAGTGGAGAACGAAAGATTTGAACCTAATTAAAATCCTTTGGATTTGAGTACATACTTGTGACATTTTAACTAAACTTTTAATGAACTTTTTGTTTTCATTTTTAAGGGATCAAAATTGTTACTTTCTTGAAATGAAAGAATCAAATATATATAAAAATGTATAGAAATGTATGAAAATGTATAGAAATGAAAATGCACATTTTTAAAATATAAAGACCAGGAGAATAGGGTTAAAAGTACAGAGATCATGAAAATTAGTAATATGATATCATTTTAAAGATCAAATAAACACAATATTATTGGTTAGGAAAGGTATATAAATAATTGAATTTAACTTGAGCATAAAGCTATCAAATTGGAATAATTTGGAGGGATCTATTTGGCATTAAAATCATTTTTATAACAAAGTTGATATAATTTGTTGTTACTCAAAATAATTGGATAAGGTCTTGGAAAAACAAGGTATAATTTATAGTAAAAAGGGAAAGAATAATTAATATAAAAAGTGAACCCACAATGTGTTTCCTAATTATATATATGGGTTAGAATTTGTAGAAAAATAATAGAAAGAGAAATAGAAATATTTGATGTGGCATTTTGTTTGAATTATTGAAGTGGAAATGATATCTATAGGGCCATATTTTGATTTAGGGTTTAATGTGGGATGTTAGAGAAAATTATACATATATATATATATTATATGATATGACAAAAAGATCAAGTAGCTTGGTTGGTTGGTGTGTAATTTAATGGGACCAAAGTTTCTAATTATTTTACTATATATATATATGCACACACACTCATATATATTAAAAAGAAATAAAAGAAGGGGTAGCATAATAGATTCTATTAAAATTAGTTGAATTTAGCTTTATAAAGCAAGAAAGTGACATGTTTTGAATTCATTCATCACTATTCAACTTTTGTGATTTGTAGTTTTCATCCAAAAGAATATTTATGTTAANNNNNNNNNNNNNNNNNNNNTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTACTAGCTAAAATCATAAATATAAAATATTTTGTTATGAGTATATTGTATATAATTTAGAAATTTATAAAAAATATTTATATTTTATAGATAAACCAAATGTATTTTTTTTTGAAATTTTTTTATTTTTTTAAAAAATCCTATTAGTTTCTACTGATAAACATTTTAGCAATGCATTCATATATATAGTAATATAATCTGAAACTTTATTATATTTTTTATTATACTATATTTAAAAATGTTCTAAAATAATTAGATTTTGAATGCATATCTTATCTATCTACCTCATAGAAAAATTATTTAAAATCACTCTCAAATCAAACTCACACCCAATGTCAAACTTAATCATAGCTCAATGAATTAGAAAACAATAATTAACTTACAAATAAGCAATTCACATTCTCAAAATACTTTTTTAATAAATGATTTTTTTTTAATTTGTAATATATAAAAAAAAGAACTAAAACGTTGAAGTGTTTTGTTTGCAAAAATAGCTACAAAATAAACTATAAAATATATTGATGAATTAATTTTGATTGTGAATATTTGTAGGTTATTTAATTTAAAGCAAAATTGTCAAAAAAAAAATTAATGAGTCTTATTGTGTTAATTAACTAAATTGTGAACGTCGGTGAAATCACAGCCGTTGATGGAGACGGATTCATCGCCAGAGACATAACTTTCAGAAACACCGCCGGAGCAAAGAACCACCAAGCGGTAGCGCTCCGATCAGGTTCAGATCTCTCAGTCTTCTACCGTTGTGGCTTCGAAGGCTATCAAGACACTCTCTACGTTTACGCCGAACGCCAATTCTACAAACAATGCGACATTTACGGTACCGTCGATTTCATCTTCGGCAATGCCGCCGTCGTACTCCAAGATTGCAACATCATCGCCCGTGATCCTCCGAACAAAACCATCACCCTCACCGCCCAAGGCCGCTCCGATCCGAATCAGAACACCGGAATCTCCATCCACAATTGCCGAATCACATCCTCTGGCGGCCTCAGCGGCGTCAAGGCCTATTTAGGCCGCCCATGGAGGCAATATTCCCGGACGGTTGTTATGAAATCCTCGATCGGAGGATTCATAAGTCCGGCGGGGTGGATGCCGTGGAGCGGTAATTTTGCCCTAAATACGCTGTATTACGCCGAGTATATGAATACGGGGCCGGGAGCGTCGACGGCGAATCGGGTGAACTGGAAGGGTTATCATGTGATTACCAGTGCGTCGGAGGCTTCGAAGTTCACCGTGGGGAATTTCATCGCCGGTGGGTCTTGGTTGCCGAGTACGGGTGTGCCTTTCACTTCCGGTCTCTAATTTGGCGGTGTAATTTTGTATGTATTATTAAAAATATTAATGATCGTTTATTTTTTTTAATTATACTTTAATAAACAAGTTTATCATTAAGAAATTAGATTTTATTATATCTTTTAAAAAAATTGATTTTACTTTTCTTTTCGTCTCTCAATTCAAAATGGTTGGGTTTGACAAGAGAATTACCATTCAAGAGGGGTATTGTATTACAATTTACAATGTAAGTTTTTCAAATAAATGAAATATGATTTGTTTGAGT

mRNA sequence

ATGGCAAGACACACGATCGTGTTTTTCGTTGTTCTTATTGTTCTTATTTTTCCGTCAGACATTTTATGTTATTCGGAAAAAGAAATAAAAAATTGGTGTAGCCAAACACCATATCCAGCACCATGTGAAGAATTTCTCAAAACCAAAGCTACTACAAAGAAAACTCCAATAACCACAAAATCCCATTTCTTTGAAATCTTAGTCGAAACGGCACTAGAGCGTGCCGTTTCAGCCCATAAAAATGCTCTTTCTCTCGGCCCAAAATGCCGAAATTCCAAAGAAAAAACTGCATGGACCGATTGCGTTGACCTCTACGACCAAATCATAACCCGACTCAACCGAACCTCTGCCCGCTGCTCACCAGCCGACGCTCAAACCTGGCTCAGCGCCGCTCTCACCGCCCTCGAAACCTGCCGGACAGGGTTCGAAGAACTCGGCCTTTCGGCTTTCGGCTACCCATTGACGGCTAATAATGTTTCCAAGCTGATTAGTGATGGGTTGTCGGTGAATAAACCCGCATCACCGGAGGGGTATGAGCCGACGACCATGACTGATGGGTTTCCCACGTGGGTTAGTCCTGGTAACCGTAAATTGCTGCAATCTGAGTCTCCGAAGGCGGACGTAGTGGTGGCGCAAGATGGGTCGGGGAATTTTAAGACGGTGAAGGATGCTATCTCCGCCGCAAAGGGCGGCGGGAGATTTGTGATATATATAAAGTCGGGGGTTTATAATGAGAATTTGGATATTAAGGCTAAGAATGTTATGATGGTCGGCGACGGAATCGGAAAAACTATCATTACCGGCAGTAGAAGTGTCGGCGGAGGTTCCACCACGTTCAGATCCGCTACCGTTGCCGTTGATGGAGACGGATTCATCGCCAGAGACATAACTTTCAGAAACACCGCCGGAGCAAAGAACCACCAAGCGGTAGCGCTCCGATCAGGTTCAGATCTCTCAGTCTTCTACCGTTGTGGCTTCGAAGGCTATCAAGACACTCTCTACGTTTACGCCGAACGCCAATTCTACAAACAATGCGACATTTACGGTACCGTCGATTTCATCTTCGGCAATGCCGCCGTCGTACTCCAAGATTGCAACATCATCGCCCGTGATCCTCCGAACAAAACCATCACCCTCACCGCCCAAGGCCGCTCCGATCCGAATCAGAACACCGGAATCTCCATCCACAATTGCCGAATCACATCCTCTGGCGGCCTCAGCGGCGTCAAGGCCTATTTAGGCCGCCCATGGAGGCAATATTCCCGGACGGTTGTTATGAAATCCTCGATCGGAGGATTCATAAGTCCGGCGGGGTGGATGCCGTGGAGCGGTAATTTTGCCCTAAATACGCTGTATTACGCCGAGTATATGAATACGGGGCCGGGAGCGTCGACGGCGAATCGGGTGAACTGGAAGGGTTATCATGTGATTACCAGTGCGTCGGAGGCTTCGAAGTTCACCGTGGGGAATTTCATCGCCGGTGGGTCTTGGTTGCCGAGTACGGGTGTGCCTTTCACTTCCGGTCTCTAATTTGGCGGTGTAATTTTGTATGtattattaaaaatattaatgatcgtttattttttttaattatactttaataaacaagtttatcattaagaaattagattttattatatcttttaaaaaaattGATTTTACTTTTCTTTTCGTCTCTCAATTCAAAATGGTTGGGTTTGACAAGAGAATTACCATTCAAGAGGGGTATTGTATTACAATTTACAATGTAAGTTTTTCAAATAAATGAAATATGATTTGTTTGAGT

Coding sequence (CDS)

ATGGCAAGACACACGATCGTGTTTTTCGTTGTTCTTATTGTTCTTATTTTTCCGTCAGACATTTTATGTTATTCGGAAAAAGAAATAAAAAATTGGTGTAGCCAAACACCATATCCAGCACCATGTGAAGAATTTCTCAAAACCAAAGCTACTACAAAGAAAACTCCAATAACCACAAAATCCCATTTCTTTGAAATCTTAGTCGAAACGGCACTAGAGCGTGCCGTTTCAGCCCATAAAAATGCTCTTTCTCTCGGCCCAAAATGCCGAAATTCCAAAGAAAAAACTGCATGGACCGATTGCGTTGACCTCTACGACCAAATCATAACCCGACTCAACCGAACCTCTGCCCGCTGCTCACCAGCCGACGCTCAAACCTGGCTCAGCGCCGCTCTCACCGCCCTCGAAACCTGCCGGACAGGGTTCGAAGAACTCGGCCTTTCGGCTTTCGGCTACCCATTGACGGCTAATAATGTTTCCAAGCTGATTAGTGATGGGTTGTCGGTGAATAAACCCGCATCACCGGAGGGGTATGAGCCGACGACCATGACTGATGGGTTTCCCACGTGGGTTAGTCCTGGTAACCGTAAATTGCTGCAATCTGAGTCTCCGAAGGCGGACGTAGTGGTGGCGCAAGATGGGTCGGGGAATTTTAAGACGGTGAAGGATGCTATCTCCGCCGCAAAGGGCGGCGGGAGATTTGTGATATATATAAAGTCGGGGGTTTATAATGAGAATTTGGATATTAAGGCTAAGAATGTTATGATGGTCGGCGACGGAATCGGAAAAACTATCATTACCGGCAGTAGAAGTGTCGGCGGAGGTTCCACCACGTTCAGATCCGCTACCGTTGCCGTTGATGGAGACGGATTCATCGCCAGAGACATAACTTTCAGAAACACCGCCGGAGCAAAGAACCACCAAGCGGTAGCGCTCCGATCAGGTTCAGATCTCTCAGTCTTCTACCGTTGTGGCTTCGAAGGCTATCAAGACACTCTCTACGTTTACGCCGAACGCCAATTCTACAAACAATGCGACATTTACGGTACCGTCGATTTCATCTTCGGCAATGCCGCCGTCGTACTCCAAGATTGCAACATCATCGCCCGTGATCCTCCGAACAAAACCATCACCCTCACCGCCCAAGGCCGCTCCGATCCGAATCAGAACACCGGAATCTCCATCCACAATTGCCGAATCACATCCTCTGGCGGCCTCAGCGGCGTCAAGGCCTATTTAGGCCGCCCATGGAGGCAATATTCCCGGACGGTTGTTATGAAATCCTCGATCGGAGGATTCATAAGTCCGGCGGGGTGGATGCCGTGGAGCGGTAATTTTGCCCTAAATACGCTGTATTACGCCGAGTATATGAATACGGGGCCGGGAGCGTCGACGGCGAATCGGGTGAACTGGAAGGGTTATCATGTGATTACCAGTGCGTCGGAGGCTTCGAAGTTCACCGTGGGGAATTTCATCGCCGGTGGGTCTTGGTTGCCGAGTACGGGTGTGCCTTTCACTTCCGGTCTCTAA

Protein sequence

MARHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL*
Homology
[Close]
BLAST of Cucsa.257480 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 5.6e-190
Identity = 326/513 (63.55%), Postives = 400/513 (77.97%), Query Frame = 1 

Query: 6   IVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHFFE 65
           I+  V L++         YS +E+K+WC +TP P PCE FL  K  T  T I   + F++
Sbjct: 5   ILITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQK--TDVTSIKQDTDFYK 64

Query: 66  ILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSAR---CSPA 125
           I ++ ALERA +A     +LG KCRN +EK AW DC +LY+  + +LN+TS     C+  
Sbjct: 65  ISLQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKV 124

Query: 126 DAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTT 185
           D QTWLS ALT LETCR   E+LG+  +  PL +NNV+KLIS+ LS+NK      Y   +
Sbjct: 125 DKQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKVP----YNEPS 184

Query: 186 MTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAK--GGGRFVIYIKS 245
             DGFPTWV PG+RKLLQ+ +P+A++VVAQDGSGN KT+++A++AA   GG R+VIYIK+
Sbjct: 185 YKDGFPTWVKPGDRKLLQT-TPRANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKA 244

Query: 246 GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN 305
           G YNEN+++K KN+M VGDGIGKTIITGS+SVGGG+TTF+SATVAV GD FIARDIT RN
Sbjct: 245 GTYNENIEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRN 304

Query: 306 TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV 365
           TAG  NHQAVALRSGSDLSVFYRC FEGYQDTLYV+++RQFY++CDIYGTVDFIFGNAAV
Sbjct: 305 TAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAV 364

Query: 366 VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGL----SGVKAYLGRP 425
           VLQ+CNI AR PPN+T TLTAQGR+DPNQ+TGI IHNCR+T++  L    S VK +LGRP
Sbjct: 365 VLQNCNIFARKPPNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRP 424

Query: 426 WRQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHV 485
           W+QYSRTV +K+ +   I+PAGWM WSG+FALNTLYYAEYMNTGPG+STANRV W+GYHV
Sbjct: 425 WKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHV 484

Query: 486 ITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           +TS S+ S+FTVGNFIAG SWLP+T VPFTSGL
Sbjct: 485 LTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

[Close]
BLAST of Cucsa.257480 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 652.9 bits (1683), Expect = 2.9e-186
Identity = 323/514 (62.84%), Postives = 397/514 (77.24%), Query Frame = 1 

Query: 3   RHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSH 62
           R  I+ FV+L +L+  S +  Y++K++K WCSQTP P PCE FL   +  +  PI ++S 
Sbjct: 5   RAYIINFVILCILV-ASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNE--PIKSESE 64

Query: 63  FFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRT---SARC 122
           F +I ++  L+RA+ A  +A +LGPKCR+++EK AW DC+ LYD  ++++N T   + +C
Sbjct: 65  FLKISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKC 124

Query: 123 SPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 182
           S  DAQTWLS ALT L+TCR GF ELG++    PL +NNVS L+ + L++NK   P  Y 
Sbjct: 125 SKLDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKV--PFNYT 184

Query: 183 PTTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIK 242
           P    DGFP+WV PG+RKLLQS +PK + VVA+DGSGNFKT+K+AI AA G GRFVIY+K
Sbjct: 185 PPEK-DGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVK 244

Query: 243 SGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 302
            GVY+ENL+I+ KNVM+ GDGIGKTIITGS+SVGGG+TTF SATVA  GDGFIAR ITFR
Sbjct: 245 QGVYSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFR 304

Query: 303 NTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 362
           NTAGA N QAVALRSGSDLSVFY+C FE YQDTLYV++ RQFY+ CD+YGTVDFIFGNAA
Sbjct: 305 NTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAA 364

Query: 363 VVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGV----KAYLGR 422
            VLQ+CNI AR P +KT T+TAQGRSDPNQNTGI IHN R+T++  L  V    K YLGR
Sbjct: 365 AVLQNCNIFARRPRSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGR 424

Query: 423 PWRQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYH 482
           PWRQYSRTV MK+S+   I P GW+ W GNFAL TL+YAE+ NTGPGAST+ RV W G+ 
Sbjct: 425 PWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFR 484

Query: 483 VITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           V+ SASEASKFTVG F+AGGSW+PS+ VPFTSGL
Sbjct: 485 VLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

[Close]
BLAST of Cucsa.257480 vs. Swiss-Prot
Match: PME59_ARATH (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=PME59 PE=2 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 7.2e-137
Identity = 262/536 (48.88%), Postives = 356/536 (66.42%), Query Frame = 1 

Query: 1   MARHTIVFF-VVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITT 60
           M + +I+F  ++L+VL+    +   +++   +WC +TPYP PC+ + K     ++   T 
Sbjct: 5   MQKLSILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQP--TQ 64

Query: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRT---- 120
            S F  +LVE A++RA+SA     + G  C +SK++    DC+DLY   I +LNRT    
Sbjct: 65  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 124

Query: 121 ------SARCSPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANN-VSKLISDGLS 180
                 +  C+  DAQTWLS ALT  ETCR G  +L ++ F  P+ +N  +S LIS+ L+
Sbjct: 125 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLA 184

Query: 181 VNKPASPEGYEPTTMTD--GFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAIS 240
           VN      G +  T  +  GFPTW+S  +++LL++   +A++VVA+DGSG+F TV+ AI 
Sbjct: 185 VNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFNTVQAAID 244

Query: 241 AAKG----GGRFVIYIKSGVYNENLDIKAKN--VMMVGDGIGKTIITGSRSVGGGSTTFR 300
            A       GRFVIY+K G+Y EN++++  N  +M+VGDG+  TIITG RSV GG TT+ 
Sbjct: 245 VAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYN 304

Query: 301 SATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQ 360
           SAT  ++G  FIA+ ITFRNTAG    QAVALRS SDLS+FY+C  EGYQDTL V+++RQ
Sbjct: 305 SATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQ 364

Query: 361 FYKQCDIYGTVDFIFGNAAVVLQDCNIIARDP-PNKTITLTAQGRSDPNQNTGISIHNCR 420
           FY++C IYGTVDFIFGNAA V Q+C I+ R P   +   +TAQGR+DP QNTGISIHN R
Sbjct: 365 FYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSR 424

Query: 421 ITSSGGL----SGVKAYLGRPWRQYSRTVVMKSSIGGFISPAGWMPW--SGNFALNTLYY 480
           I  +  L      VK Y+GRPW ++SRTVV+++ +   +SP GW PW     F L+TL+Y
Sbjct: 425 ILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFY 484

Query: 481 AEYMNTGPGASTANRVNWKGYHVITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           AEY NTGP +ST  RV+WKG+HV+  AS+AS FTVG FIAG +WLP TG+PFTSGL
Sbjct: 485 AEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]
BLAST of Cucsa.257480 vs. Swiss-Prot
Match: PME60_ARATH (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana GN=PME60 PE=2 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 6.7e-135
Identity = 261/506 (51.58%), Postives = 334/506 (66.01%), Query Frame = 1 

Query: 29  IKNWCSQTPYPAPCEE-FLKTKATTKKTPITTKSHFFEILVETALERAVSAHKNALSLGP 88
           I  WC +TPYP PC+  F+K       T I   S F  +LVE A++RAVSA     +   
Sbjct: 38  IDGWCDKTPYPYPCKRYFIKHSGFRLPTQI---SEFRVLLVEAAMDRAVSAWDKLTNSSK 97

Query: 89  KCRNSKEKTAWTDCVDLYDQIITRLNRT--------SARCSPADAQTWLSAALTALETCR 148
            C + K++    DC++LY   + +LNRT          RC+  DAQTWLS ALT  ETCR
Sbjct: 98  NCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRRCTDFDAQTWLSTALTNTETCR 157

Query: 149 TGFEELGLSAFGYPLTANN-VSKLISDGLSVNKPASPEGYEPTTMTD--GFPTWVSPGNR 208
            G  +L +S F  P+ +N  +S LIS+ L+VN      G   +T  D  GFPTWVS   R
Sbjct: 158 RGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTAGKNDSTTGDSKGFPTWVSRKER 217

Query: 209 KLLQSESPKADVVVAQDGSGNFKTVKDAISAAKG----GGRFVIYIKSGVYNENLDIKAK 268
           +LLQ +S +A++VVA+DGSG+FKTV+ AI  A       GRFVIY+K G+Y ENL+++  
Sbjct: 218 RLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTSGRFVIYVKRGIYQENLNVRLN 277

Query: 269 N--VMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAV 328
           N  +M+VGDG+  TIITG RSV GG TT+ SAT  ++G  FIA+ I F+NTAG    QAV
Sbjct: 278 NDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEGLHFIAKGIAFQNTAGPAKGQAV 337

Query: 329 ALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQDCNIIAR 388
           ALRS SDLS+FYRC  EGYQDTL V+++RQFY++C IYGTVDFIFGNAAVV Q+C I+ R
Sbjct: 338 ALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIYGTVDFIFGNAAVVFQNCIILPR 397

Query: 389 DP-PNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLS----GVKAYLGRPWRQYSRTVV 448
            P   +   +TAQGR+D  QNTGISIHN  I  +  L      VK Y+GRPW  YSRTVV
Sbjct: 398 LPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVV 457

Query: 449 MKSSIGGFISPAGWMPWS--GNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSASEA 508
           +K+ I   +SP GW PW+    + L+TL+YAEY N GP +ST  RV WKG+HV++ AS+A
Sbjct: 458 LKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGPASSTRWRVRWKGFHVLSKASDA 517

Query: 509 SKFTVGNFIAGGSWLPSTGVPFTSGL 510
           S F+VG FIAG +WLP +G+PFTS L
Sbjct: 518 SAFSVGKFIAGTAWLPGSGIPFTSEL 540

[Close]
BLAST of Cucsa.257480 vs. Swiss-Prot
Match: PME33_ARATH (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana GN=PME33 PE=2 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.7e-130
Identity = 250/508 (49.21%), Postives = 330/508 (64.96%), Query Frame = 1 

Query: 32  WCSQTPYPAPCEEFLKTKATTKKT--PITTKSHFFEILVETALERAVSAHKNALSLGPKC 91
           WC++TP+   C  + +  +       P   +S F  +LV+ AL++AV  H   +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 92  RNSKEKTAWTDCVDLYDQIITRLNRT----------SARCSPADAQTWLSAALTALETCR 151
            N++ K AW+DCV+L+   + +LNRT            +C+  DAQTWLS A T +ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 152 TGFEELGLSAFGYPLTAN-NVSKLISDGLSVNKPASPEGYEPTTMT--DGFPTWVSPGNR 211
           +G E+L +S F  P+ +N N+S LI + L+VN     +    TT    + FP+WVS   R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 212 KLLQSES---PKADVVVAQDGSGNFKTVKDAISAA---KGGGRFVIYIKSGVYNENLDI- 271
           +LL S S       +VVAQD SG+F++++ AI+ A   +   RFVIY+K GVY EN+D+ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 272 -KAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQ 331
               N+M+VGDG  KTIIT  RSV  G TT+ SAT    G  F+A+D+TF NTAG    Q
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLRGQ 386

Query: 332 AVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQDCNII 391
           AVA+RS SDLSVFYR G  G+QDTLY++++RQF+++C I GT+DFIFGNAAVV Q+C I+
Sbjct: 387 AVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCMIL 446

Query: 392 ARDP-PNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGV----KAYLGRPWRQYSRT 451
            R P   +   +TAQGR DP QNTGI+IH+ RI ++  L  V    K YLGRPW+ YSR 
Sbjct: 447 VRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYSRV 506

Query: 452 VVMKSSIGGFISPAGWMPW--SGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSAS 510
            +MK+ I   ISP GW PW    NFALNT++Y EY N GPG+ST  RV WKG+H ITS +
Sbjct: 507 TIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITSTA 566

[Close]
BLAST of Cucsa.257480 vs. TrEMBL
Match: A0A0A0L7N4_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G126810 PE=4 SV=1)

HSP 1 Score: 1036.2 bits (2678), Expect = 1.3e-299
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 1 

Query: 1   MARHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK 60
           MARHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK
Sbjct: 1   MARHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK 60

Query: 61  SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCS 120
           SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCS
Sbjct: 61  SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCS 120

Query: 121 PADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEP 180
           PADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEP
Sbjct: 121 PADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEP 180

Query: 181 TTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKS 240
           TTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKS
Sbjct: 181 TTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKS 240

Query: 241 GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN 300
           GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN
Sbjct: 241 GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN 300

Query: 301 TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV 360
           TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV
Sbjct: 301 TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV 360

Query: 361 VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQY 420
           VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQY
Sbjct: 361 VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQY 420

Query: 421 SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA 480
           SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA
Sbjct: 421 SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA 480

Query: 481 SEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           SEASKFTVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 481 SEASKFTVGNFIAGGSWLPSTGVPFTSGL 509

[Close]
BLAST of Cucsa.257480 vs. TrEMBL
Match: W9R8N4_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_025594 PE=4 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 6.7e-198
Identity = 342/512 (66.80%), Postives = 414/512 (80.86%), Query Frame = 1 

Query: 6   IVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHFFE 65
           I   +  ++L     IL YS +E+K+WC QTP P PCE FL       +TPI  KS FF+
Sbjct: 12  ITLSIFFLILYLSPTILGYSLQEVKSWCGQTPNPQPCEYFLSNNLEYDRTPIKQKSDFFK 71

Query: 66  ILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTS---ARCSPA 125
           +  + AL+RA+ AH +  S+GPKCR+ +EK AW DC++LY+  + RLN+TS   ++C+  
Sbjct: 72  LSAKLALDRAMHAHDDLFSVGPKCRSQQEKAAWADCLELYESTVLRLNKTSKPTSKCTQD 131

Query: 126 DAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTT 185
           D QTWLS ALT LETCR G  ELG+S +  PL +NNVSKLIS+ L++NK  SP  Y+P  
Sbjct: 132 DLQTWLSTALTNLETCRAGLLELGVSDYLLPLMSNNVSKLISNSLAINK--SP--YKPDN 191

Query: 186 M-TDGFPTWVSPGNRKLLQSESP---KADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYI 245
              +GFPTWV PG+R+LLQS S    +A+VVVA+DGSG++ TVK A++A KG GRFVIY+
Sbjct: 192 KYNNGFPTWVKPGDRRLLQSSSSPASRANVVVAKDGSGDYTTVKAAVTAVKGSGRFVIYV 251

Query: 246 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 305
           K+G YNEN++IK KNVM++GDGIGKTIITGS+SVGGGSTTF+SATVAV GDGFIA+DITF
Sbjct: 252 KAGTYNENVEIKVKNVMLLGDGIGKTIITGSKSVGGGSTTFQSATVAVVGDGFIAQDITF 311

Query: 306 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 365
           RNTAGA NHQAVALRSGSD SVFY+C FEGYQDTLYV++ERQFY++CDIYGTVDFIFGNA
Sbjct: 312 RNTAGASNHQAVALRSGSDQSVFYKCSFEGYQDTLYVHSERQFYRECDIYGTVDFIFGNA 371

Query: 366 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGL-SGVKAYLGRPW 425
           AVVLQ+CNI ARDPPNKT T+TAQGR+D NQNTGISIHNCR+T++  L S VK YLGRPW
Sbjct: 372 AVVLQNCNIYARDPPNKTNTITAQGRTDSNQNTGISIHNCRVTAASSLSSSVKTYLGRPW 431

Query: 426 RQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVI 485
           ++YSRTVVMK+++G  I+PAGW+ WSGNFAL TLYYAEY NTGPG+ST+NRV W GYHVI
Sbjct: 432 KEYSRTVVMKTNLGSLINPAGWLEWSGNFALKTLYYAEYANTGPGSSTSNRVKWTGYHVI 491

Query: 486 TSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
             ASEASKFTVGNFIAGGSWLP+T VP+TSGL
Sbjct: 492 -GASEASKFTVGNFIAGGSWLPATNVPYTSGL 518

[Close]
BLAST of Cucsa.257480 vs. TrEMBL
Match: A0A0S3SY54_PHAAN (Pectinesterase OS=Vigna angularis var. angularis GN=Vigan.09G122000 PE=4 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 3.1e-195
Identity = 334/513 (65.11%), Postives = 412/513 (80.31%), Query Frame = 1 

Query: 5   TIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHFF 64
           +++  ++LI L+F S +  YS K+I++WC+QTPYP PCE +L   A  K  PI +KS F 
Sbjct: 14  SLLISLLLIPLLFSSVVFSYSLKDIQSWCNQTPYPQPCEYYLTNHAFNK--PIKSKSDFL 73

Query: 65  EILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTS---ARCSP 124
           ++ ++ ALERA     N  S+GPKCRN  EK+AW DC+ LY   I RLN+T     +C+ 
Sbjct: 74  KVSLQLALERAERGQLNTHSIGPKCRNDHEKSAWNDCIQLYQYTIQRLNKTLDPYTKCTQ 133

Query: 125 ADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 184
            D QTWLS ALT LETCR GF ELG++ +  PL +NNV+KL+S+ LS+NK  + + ++P 
Sbjct: 134 TDTQTWLSTALTNLETCRIGFYELGVTDYILPLLSNNVTKLLSNTLSLNKDPNSQ-FKPP 193

Query: 185 TMTDGFPTWVSPGNRKLLQSESP--KADVVVAQDGSGNFKTVKDAISAA-KGGGRFVIYI 244
           +  +GFPTWV PG+RKLLQS S   KA+V+VA+DGSG + TVK A+ AA    GR+VIY+
Sbjct: 194 STQEGFPTWVKPGDRKLLQSSSAASKANVIVAKDGSGKYTTVKAAVDAAPSSSGRYVIYV 253

Query: 245 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 304
           KSGVYNE +++K+KN+M+VGDGIGKTIITGS+SVGGGSTTF SATVA  GDGFIA+DITF
Sbjct: 254 KSGVYNEQVEVKSKNIMLVGDGIGKTIITGSKSVGGGSTTFNSATVAAVGDGFIAQDITF 313

Query: 305 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 364
           RNTAGA NHQAVALRSGSDLSVFYRC FEGYQDTLYV++ERQFY++CDIYGTVDFIFGNA
Sbjct: 314 RNTAGAANHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSERQFYRECDIYGTVDFIFGNA 373

Query: 365 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGL--SGVKAYLGRP 424
           AVVLQ+CNI AR PP KTIT+TAQGR+DPNQNTGI IHN ++T + G   S VK+YLGRP
Sbjct: 374 AVVLQNCNIYARTPPQKTITVTAQGRTDPNQNTGIIIHNSKVTGASGFNPSSVKSYLGRP 433

Query: 425 WRQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHV 484
           W+QYSRTV MK+++   I+PAGWM W GNFAL+TLYYAEY NTGPG+STANRV WKGYH+
Sbjct: 434 WKQYSRTVFMKTNLDSLINPAGWMEWDGNFALDTLYYAEYANTGPGSSTANRVTWKGYHI 493

Query: 485 ITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           +T+AS+A+ FTVGNFIAG SW+P+TGVPFTSGL
Sbjct: 494 LTTASQATPFTVGNFIAGNSWIPNTGVPFTSGL 523

[Close]
BLAST of Cucsa.257480 vs. TrEMBL
Match: A0A0L9VS61_PHAAN (Pectinesterase OS=Phaseolus angularis GN=LR48_Vigan11g061200 PE=4 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 3.1e-195
Identity = 334/513 (65.11%), Postives = 412/513 (80.31%), Query Frame = 1 

Query: 5   TIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHFF 64
           +++  ++LI L+F S +  YS K+I++WC+QTPYP PCE +L   A  K  PI +KS F 
Sbjct: 14  SLLISLLLIPLLFSSVVFSYSLKDIQSWCNQTPYPQPCEYYLTNHAFNK--PIKSKSDFL 73

Query: 65  EILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTS---ARCSP 124
           ++ ++ ALERA     N  S+GPKCRN  EK+AW DC+ LY   I RLN+T     +C+ 
Sbjct: 74  KVSLQLALERAERGQLNTHSIGPKCRNDHEKSAWNDCIQLYQYTIQRLNKTLDPYTKCTQ 133

Query: 125 ADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 184
            D QTWLS ALT LETCR GF ELG++ +  PL +NNV+KL+S+ LS+NK  + + ++P 
Sbjct: 134 TDTQTWLSTALTNLETCRIGFYELGVTDYILPLLSNNVTKLLSNTLSLNKDPNSQ-FKPP 193

Query: 185 TMTDGFPTWVSPGNRKLLQSESP--KADVVVAQDGSGNFKTVKDAISAA-KGGGRFVIYI 244
           +  +GFPTWV PG+RKLLQS S   KA+V+VA+DGSG + TVK A+ AA    GR+VIY+
Sbjct: 194 STQEGFPTWVKPGDRKLLQSSSAASKANVIVAKDGSGKYTTVKAAVDAAPSSSGRYVIYV 253

Query: 245 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 304
           KSGVYNE +++K+KN+M+VGDGIGKTIITGS+SVGGGSTTF SATVA  GDGFIA+DITF
Sbjct: 254 KSGVYNEQVEVKSKNIMLVGDGIGKTIITGSKSVGGGSTTFNSATVAAVGDGFIAQDITF 313

Query: 305 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 364
           RNTAGA NHQAVALRSGSDLSVFYRC FEGYQDTLYV++ERQFY++CDIYGTVDFIFGNA
Sbjct: 314 RNTAGAANHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSERQFYRECDIYGTVDFIFGNA 373

Query: 365 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGL--SGVKAYLGRP 424
           AVVLQ+CNI AR PP KTIT+TAQGR+DPNQNTGI IHN ++T + G   S VK+YLGRP
Sbjct: 374 AVVLQNCNIYARTPPQKTITVTAQGRTDPNQNTGIIIHNSKVTGASGFNPSSVKSYLGRP 433

Query: 425 WRQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHV 484
           W+QYSRTV MK+++   I+PAGWM W GNFAL+TLYYAEY NTGPG+STANRV WKGYH+
Sbjct: 434 WKQYSRTVFMKTNLDSLINPAGWMEWDGNFALDTLYYAEYANTGPGSSTANRVTWKGYHI 493

Query: 485 ITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           +T+AS+A+ FTVGNFIAG SW+P+TGVPFTSGL
Sbjct: 494 LTTASQATPFTVGNFIAGNSWIPNTGVPFTSGL 523

[Close]
BLAST of Cucsa.257480 vs. TrEMBL
Match: I1JKR9_SOYBN (Pectinesterase OS=Glycine max GN=GLYMA_03G029200 PE=4 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 3.4e-194
Identity = 332/510 (65.10%), Postives = 402/510 (78.82%), Query Frame = 1 

Query: 7   VFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHFFEI 66
           +F  + +     S +  YS  ++K WCSQTP P PCE FL    T +  PI  KS FF++
Sbjct: 6   LFLTLSVPFFLSSFVSSYSWNDVKLWCSQTPNPEPCEYFLSNNPTHQYKPIKQKSDFFKL 65

Query: 67  LVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRT---SARCSPAD 126
            ++ A ERA++ H N LSLG KCRN +E  AW DCV+LY+Q I +LN+T   S + S  D
Sbjct: 66  SLQLAQERALNGHANTLSLGSKCRNPRETAAWADCVELYEQTIRKLNKTLDPSTKFSQVD 125

Query: 127 AQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTM 186
            QTWLS ALT LETC+ GF ELG+  +  PL +NNV+KL+S+ L++NK      YE  + 
Sbjct: 126 TQTWLSTALTNLETCKAGFYELGVQDYVLPLMSNNVTKLLSNTLALNKVE----YEEPSY 185

Query: 187 TDGFPTWVSPGNRKLLQSESP--KADVVVAQDGSGNFKTVKDAISAA--KGGGRFVIYIK 246
            DGFPTWV PG+R+LLQ+ SP  KA+VVVA+DGSG + TV +A++AA     GR+VIY+K
Sbjct: 186 KDGFPTWVKPGDRRLLQASSPASKANVVVAKDGSGKYTTVSEAVNAAPKSNSGRYVIYVK 245

Query: 247 SGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 306
            G+Y+E ++IKA N+M+VGDGIGKTIIT S+SVGGG+TTFRSATVAV GDGFI +DITFR
Sbjct: 246 GGIYDEQVEIKANNIMLVGDGIGKTIITSSKSVGGGTTTFRSATVAVVGDGFITQDITFR 305

Query: 307 NTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 366
           NTAGA NHQAVALRSGSDLSVFYRC FEGYQDTLYVY++RQFY++CDIYGTVDFIFGNAA
Sbjct: 306 NTAGATNHQAVALRSGSDLSVFYRCSFEGYQDTLYVYSDRQFYRECDIYGTVDFIFGNAA 365

Query: 367 VVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQ 426
           VV Q+CNI AR+PPNK  T+TAQGR+DPNQNTGISIHN ++T++  L GV+ YLGRPW+Q
Sbjct: 366 VVFQNCNIYARNPPNKVNTITAQGRTDPNQNTGISIHNSKVTAASDLMGVRTYLGRPWQQ 425

Query: 427 YSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITS 486
           YSRTV MK+ +   I+P GW+ WSGNFAL+TLYY EYMNTGPG+STANRVNW GYHVITS
Sbjct: 426 YSRTVFMKTYLDSLINPEGWLEWSGNFALSTLYYGEYMNTGPGSSTANRVNWLGYHVITS 485

Query: 487 ASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           ASEASKFTVGNFIAG SWLP+T VPFTSGL
Sbjct: 486 ASEASKFTVGNFIAGNSWLPATSVPFTSGL 511

[Close]
BLAST of Cucsa.257480 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 652.9 bits (1683), Expect = 1.6e-187
Identity = 323/514 (62.84%), Postives = 397/514 (77.24%), Query Frame = 1 

Query: 3   RHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSH 62
           R  I+ FV+L +L+  S +  Y++K++K WCSQTP P PCE FL   +  +  PI ++S 
Sbjct: 5   RAYIINFVILCILV-ASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNE--PIKSESE 64

Query: 63  FFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRT---SARC 122
           F +I ++  L+RA+ A  +A +LGPKCR+++EK AW DC+ LYD  ++++N T   + +C
Sbjct: 65  FLKISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKC 124

Query: 123 SPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 182
           S  DAQTWLS ALT L+TCR GF ELG++    PL +NNVS L+ + L++NK   P  Y 
Sbjct: 125 SKLDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKV--PFNYT 184

Query: 183 PTTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIK 242
           P    DGFP+WV PG+RKLLQS +PK + VVA+DGSGNFKT+K+AI AA G GRFVIY+K
Sbjct: 185 PPEK-DGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVK 244

Query: 243 SGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 302
            GVY+ENL+I+ KNVM+ GDGIGKTIITGS+SVGGG+TTF SATVA  GDGFIAR ITFR
Sbjct: 245 QGVYSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFR 304

Query: 303 NTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 362
           NTAGA N QAVALRSGSDLSVFY+C FE YQDTLYV++ RQFY+ CD+YGTVDFIFGNAA
Sbjct: 305 NTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAA 364

Query: 363 VVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGV----KAYLGR 422
            VLQ+CNI AR P +KT T+TAQGRSDPNQNTGI IHN R+T++  L  V    K YLGR
Sbjct: 365 AVLQNCNIFARRPRSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGR 424

Query: 423 PWRQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYH 482
           PWRQYSRTV MK+S+   I P GW+ W GNFAL TL+YAE+ NTGPGAST+ RV W G+ 
Sbjct: 425 PWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFR 484

Query: 483 VITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           V+ SASEASKFTVG F+AGGSW+PS+ VPFTSGL
Sbjct: 485 VLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

[Close]
BLAST of Cucsa.257480 vs. TAIR10
Match: AT5G51490.1 (AT5G51490.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 488.8 bits (1257), Expect = 4.1e-138
Identity = 262/536 (48.88%), Postives = 356/536 (66.42%), Query Frame = 1 

Query: 1   MARHTIVFF-VVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITT 60
           M + +I+F  ++L+VL+    +   +++   +WC +TPYP PC+ + K     ++   T 
Sbjct: 5   MQKLSILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQP--TQ 64

Query: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRT---- 120
            S F  +LVE A++RA+SA     + G  C +SK++    DC+DLY   I +LNRT    
Sbjct: 65  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 124

Query: 121 ------SARCSPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANN-VSKLISDGLS 180
                 +  C+  DAQTWLS ALT  ETCR G  +L ++ F  P+ +N  +S LIS+ L+
Sbjct: 125 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLA 184

Query: 181 VNKPASPEGYEPTTMTD--GFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAIS 240
           VN      G +  T  +  GFPTW+S  +++LL++   +A++VVA+DGSG+F TV+ AI 
Sbjct: 185 VNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFNTVQAAID 244

Query: 241 AAKG----GGRFVIYIKSGVYNENLDIKAKN--VMMVGDGIGKTIITGSRSVGGGSTTFR 300
            A       GRFVIY+K G+Y EN++++  N  +M+VGDG+  TIITG RSV GG TT+ 
Sbjct: 245 VAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYN 304

Query: 301 SATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQ 360
           SAT  ++G  FIA+ ITFRNTAG    QAVALRS SDLS+FY+C  EGYQDTL V+++RQ
Sbjct: 305 SATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQ 364

Query: 361 FYKQCDIYGTVDFIFGNAAVVLQDCNIIARDP-PNKTITLTAQGRSDPNQNTGISIHNCR 420
           FY++C IYGTVDFIFGNAA V Q+C I+ R P   +   +TAQGR+DP QNTGISIHN R
Sbjct: 365 FYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSR 424

Query: 421 ITSSGGL----SGVKAYLGRPWRQYSRTVVMKSSIGGFISPAGWMPW--SGNFALNTLYY 480
           I  +  L      VK Y+GRPW ++SRTVV+++ +   +SP GW PW     F L+TL+Y
Sbjct: 425 ILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFY 484

Query: 481 AEYMNTGPGASTANRVNWKGYHVITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           AEY NTGP +ST  RV+WKG+HV+  AS+AS FTVG FIAG +WLP TG+PFTSGL
Sbjct: 485 AEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]
BLAST of Cucsa.257480 vs. TAIR10
Match: AT5G51500.1 (AT5G51500.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 482.3 bits (1240), Expect = 3.8e-136
Identity = 261/506 (51.58%), Postives = 334/506 (66.01%), Query Frame = 1 

Query: 29  IKNWCSQTPYPAPCEE-FLKTKATTKKTPITTKSHFFEILVETALERAVSAHKNALSLGP 88
           I  WC +TPYP PC+  F+K       T I   S F  +LVE A++RAVSA     +   
Sbjct: 38  IDGWCDKTPYPYPCKRYFIKHSGFRLPTQI---SEFRVLLVEAAMDRAVSAWDKLTNSSK 97

Query: 89  KCRNSKEKTAWTDCVDLYDQIITRLNRT--------SARCSPADAQTWLSAALTALETCR 148
            C + K++    DC++LY   + +LNRT          RC+  DAQTWLS ALT  ETCR
Sbjct: 98  NCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRRCTDFDAQTWLSTALTNTETCR 157

Query: 149 TGFEELGLSAFGYPLTANN-VSKLISDGLSVNKPASPEGYEPTTMTD--GFPTWVSPGNR 208
            G  +L +S F  P+ +N  +S LIS+ L+VN      G   +T  D  GFPTWVS   R
Sbjct: 158 RGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTAGKNDSTTGDSKGFPTWVSRKER 217

Query: 209 KLLQSESPKADVVVAQDGSGNFKTVKDAISAAKG----GGRFVIYIKSGVYNENLDIKAK 268
           +LLQ +S +A++VVA+DGSG+FKTV+ AI  A       GRFVIY+K G+Y ENL+++  
Sbjct: 218 RLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTSGRFVIYVKRGIYQENLNVRLN 277

Query: 269 N--VMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAV 328
           N  +M+VGDG+  TIITG RSV GG TT+ SAT  ++G  FIA+ I F+NTAG    QAV
Sbjct: 278 NDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEGLHFIAKGIAFQNTAGPAKGQAV 337

Query: 329 ALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQDCNIIAR 388
           ALRS SDLS+FYRC  EGYQDTL V+++RQFY++C IYGTVDFIFGNAAVV Q+C I+ R
Sbjct: 338 ALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIYGTVDFIFGNAAVVFQNCIILPR 397

Query: 389 DP-PNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLS----GVKAYLGRPWRQYSRTVV 448
            P   +   +TAQGR+D  QNTGISIHN  I  +  L      VK Y+GRPW  YSRTVV
Sbjct: 398 LPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVV 457

Query: 449 MKSSIGGFISPAGWMPWS--GNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSASEA 508
           +K+ I   +SP GW PW+    + L+TL+YAEY N GP +ST  RV WKG+HV++ AS+A
Sbjct: 458 LKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGPASSTRWRVRWKGFHVLSKASDA 517

Query: 509 SKFTVGNFIAGGSWLPSTGVPFTSGL 510
           S F+VG FIAG +WLP +G+PFTS L
Sbjct: 518 SAFSVGKFIAGTAWLPGSGIPFTSEL 540

[Close]
BLAST of Cucsa.257480 vs. TAIR10
Match: AT3G47400.1 (AT3G47400.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 467.6 bits (1202), Expect = 9.7e-132
Identity = 250/508 (49.21%), Postives = 330/508 (64.96%), Query Frame = 1 

Query: 32  WCSQTPYPAPCEEFLKTKATTKKT--PITTKSHFFEILVETALERAVSAHKNALSLGPKC 91
           WC++TP+   C  + +  +       P   +S F  +LV+ AL++AV  H   +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 92  RNSKEKTAWTDCVDLYDQIITRLNRT----------SARCSPADAQTWLSAALTALETCR 151
            N++ K AW+DCV+L+   + +LNRT            +C+  DAQTWLS A T +ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 152 TGFEELGLSAFGYPLTAN-NVSKLISDGLSVNKPASPEGYEPTTMT--DGFPTWVSPGNR 211
           +G E+L +S F  P+ +N N+S LI + L+VN     +    TT    + FP+WVS   R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 212 KLLQSES---PKADVVVAQDGSGNFKTVKDAISAA---KGGGRFVIYIKSGVYNENLDI- 271
           +LL S S       +VVAQD SG+F++++ AI+ A   +   RFVIY+K GVY EN+D+ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 272 -KAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQ 331
               N+M+VGDG  KTIIT  RSV  G TT+ SAT    G  F+A+D+TF NTAG    Q
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLRGQ 386

Query: 332 AVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQDCNII 391
           AVA+RS SDLSVFYR G  G+QDTLY++++RQF+++C I GT+DFIFGNAAVV Q+C I+
Sbjct: 387 AVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCMIL 446

Query: 392 ARDP-PNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGV----KAYLGRPWRQYSRT 451
            R P   +   +TAQGR DP QNTGI+IH+ RI ++  L  V    K YLGRPW+ YSR 
Sbjct: 447 VRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYSRV 506

Query: 452 VVMKSSIGGFISPAGWMPW--SGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSAS 510
            +MK+ I   ISP GW PW    NFALNT++Y EY N GPG+ST  RV WKG+H ITS +
Sbjct: 507 TIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITSTA 566

[Close]
BLAST of Cucsa.257480 vs. TAIR10
Match: AT4G00190.1 (AT4G00190.1 pectin methylesterase 38)

HSP 1 Score: 461.5 bits (1186), Expect = 6.9e-130
Identity = 244/489 (49.90%), Postives = 319/489 (65.24%), Query Frame = 1 

Query: 33  CSQTPYPAPCEEFLKTKATTKKTPITTKSHFFEILVETALERAVSAHKNALSLGPKCRNS 92
           C +TP+P  C+  L      K  PI + + F ++ VE  L+ AV A  +   L P+  + 
Sbjct: 8   CDETPHPGECKTLL-----IKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS- 67

Query: 93  KEKTAWTDCVDLYDQIITRLNRT----SARCSPADAQTWLSAALTALETCRTGFEELGLS 152
             K AW +C+DLY+Q I RLN +       CS +D Q WLS ALT L+TC+    ELG+S
Sbjct: 68  --KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELGVS 127

Query: 153 AFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTMTDGFPTWVSPGNRKLLQSESPKADV 212
           +     +  +++  + + L++NK     G E           +S    K L S   K DV
Sbjct: 128 SH----SLESITIDVINTLAINKRMEQNGKE---------FGISKITMKTL-SIGEKVDV 187

Query: 213 VVAQDGSGNFKTVKDAISAA----KGGGRFVIYIKSGVYNE--NLDIKAKNVMMVGDGIG 272
           VVAQDGSG++KT+++A++ A    KG  R+VI++K GVY E  N+ IK+ N+M+ GDGIG
Sbjct: 188 VVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMITGDGIG 247

Query: 273 KTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFY 332
           KTIITG +S G G +T++SAT   +GDGF+ RDIT RNTAG +NHQAVALRS SD+SVFY
Sbjct: 248 KTIITGDKSKGRGFSTYKSATFVAEGDGFVGRDITIRNTAGPENHQAVALRSNSDMSVFY 307

Query: 333 RCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQDCNIIARDPPNKTITLTAQ 392
           RC  EGYQDTLYV++ RQF+++CDIYGTVDFIFGNAA VLQ+C I AR+PPN   T+TAQ
Sbjct: 308 RCSIEGYQDTLYVHSGRQFFRECDIYGTVDFIFGNAAAVLQNCRIFARNPPNGVNTITAQ 367

Query: 393 GRSDPNQNTGISIHNCRITSSGG--LSGVKAYLGRPWRQYSRTVVMKSSIGGFISPAGWM 452
            R +PNQ TGI IHN  +  + G  L GVK YLGRPWR Y+RTVV+ + +   I P GW+
Sbjct: 368 SRFNPNQTTGIVIHNSVVKGAPGVQLGGVKTYLGRPWRSYARTVVIGTYLDTLIEPNGWI 427

Query: 453 PWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSASEASKFTVGNFIAGGSWLPS 510
            W    AL+TLYY EY N+GPG+ T NRV+W G+HVI+   EA +FT+  FI   SWLP 
Sbjct: 428 DWDNVTALSTLYYGEYQNSGPGSGTENRVDWAGFHVISDIQEAREFTLPKFIDSASWLPP 474

[Close]
BLAST of Cucsa.257480 vs. NCBI nr
Match: gi|449432283|ref|XP_004133929.1| (PREDICTED: pectinesterase 2 [Cucumis sativus])

HSP 1 Score: 1036.2 bits (2678), Expect = 1.9e-299
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 1 

Query: 1   MARHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK 60
           MARHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK
Sbjct: 1   MARHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK 60

Query: 61  SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCS 120
           SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCS
Sbjct: 61  SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCS 120

Query: 121 PADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEP 180
           PADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEP
Sbjct: 121 PADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEP 180

Query: 181 TTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKS 240
           TTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKS
Sbjct: 181 TTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKS 240

Query: 241 GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN 300
           GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN
Sbjct: 241 GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN 300

Query: 301 TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV 360
           TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV
Sbjct: 301 TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV 360

Query: 361 VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQY 420
           VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQY
Sbjct: 361 VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQY 420

Query: 421 SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA 480
           SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA
Sbjct: 421 SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA 480

Query: 481 SEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           SEASKFTVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 481 SEASKFTVGNFIAGGSWLPSTGVPFTSGL 509

[Close]
BLAST of Cucsa.257480 vs. NCBI nr
Match: gi|659075527|ref|XP_008438193.1| (PREDICTED: pectinesterase 2 [Cucumis melo])

HSP 1 Score: 989.9 bits (2558), Expect = 1.6e-285
Identity = 485/511 (94.91%), Postives = 501/511 (98.04%), Query Frame = 1 

Query: 1   MARHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKK-TPITT 60
           MARH+IVF VVL+VLIFP D+LCYSEKEIKNWCSQTPYPAPCEEFLKTKA+TKK TPITT
Sbjct: 1   MARHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITT 60

Query: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARC 120
           KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYD+IITRLNRTS RC
Sbjct: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRC 120

Query: 121 SPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 180
           +PADAQTWLSAALTALETCRTGF+ELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE
Sbjct: 121 TPADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 180

Query: 181 PTT-MTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYI 240
           PTT M DGFPTWV PG+RKLLQS SPKA+VVVAQDGSGNFKTVK+A++AAKGGGRFVIY+
Sbjct: 181 PTTIMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYV 240

Query: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300
           KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300

Query: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360

Query: 361 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWR 420
           AVVLQDCNIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCRITS+GGLSGVKAYLGRPWR
Sbjct: 361 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 420

Query: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVIT 480
           QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYY EYMNTGPGASTANRVNWKGY VIT
Sbjct: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 480

Query: 481 SASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           SA+EASKFTVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 481 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

[Close]
BLAST of Cucsa.257480 vs. NCBI nr
Match: gi|703108167|ref|XP_010098938.1| (Pectinesterase 2 [Morus notabilis])

HSP 1 Score: 698.4 bits (1801), Expect = 9.6e-198
Identity = 342/512 (66.80%), Postives = 414/512 (80.86%), Query Frame = 1 

Query: 6   IVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHFFE 65
           I   +  ++L     IL YS +E+K+WC QTP P PCE FL       +TPI  KS FF+
Sbjct: 12  ITLSIFFLILYLSPTILGYSLQEVKSWCGQTPNPQPCEYFLSNNLEYDRTPIKQKSDFFK 71

Query: 66  ILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTS---ARCSPA 125
           +  + AL+RA+ AH +  S+GPKCR+ +EK AW DC++LY+  + RLN+TS   ++C+  
Sbjct: 72  LSAKLALDRAMHAHDDLFSVGPKCRSQQEKAAWADCLELYESTVLRLNKTSKPTSKCTQD 131

Query: 126 DAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTT 185
           D QTWLS ALT LETCR G  ELG+S +  PL +NNVSKLIS+ L++NK  SP  Y+P  
Sbjct: 132 DLQTWLSTALTNLETCRAGLLELGVSDYLLPLMSNNVSKLISNSLAINK--SP--YKPDN 191

Query: 186 M-TDGFPTWVSPGNRKLLQSESP---KADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYI 245
              +GFPTWV PG+R+LLQS S    +A+VVVA+DGSG++ TVK A++A KG GRFVIY+
Sbjct: 192 KYNNGFPTWVKPGDRRLLQSSSSPASRANVVVAKDGSGDYTTVKAAVTAVKGSGRFVIYV 251

Query: 246 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 305
           K+G YNEN++IK KNVM++GDGIGKTIITGS+SVGGGSTTF+SATVAV GDGFIA+DITF
Sbjct: 252 KAGTYNENVEIKVKNVMLLGDGIGKTIITGSKSVGGGSTTFQSATVAVVGDGFIAQDITF 311

Query: 306 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 365
           RNTAGA NHQAVALRSGSD SVFY+C FEGYQDTLYV++ERQFY++CDIYGTVDFIFGNA
Sbjct: 312 RNTAGASNHQAVALRSGSDQSVFYKCSFEGYQDTLYVHSERQFYRECDIYGTVDFIFGNA 371

Query: 366 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGL-SGVKAYLGRPW 425
           AVVLQ+CNI ARDPPNKT T+TAQGR+D NQNTGISIHNCR+T++  L S VK YLGRPW
Sbjct: 372 AVVLQNCNIYARDPPNKTNTITAQGRTDSNQNTGISIHNCRVTAASSLSSSVKTYLGRPW 431

Query: 426 RQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVI 485
           ++YSRTVVMK+++G  I+PAGW+ WSGNFAL TLYYAEY NTGPG+ST+NRV W GYHVI
Sbjct: 432 KEYSRTVVMKTNLGSLINPAGWLEWSGNFALKTLYYAEYANTGPGSSTSNRVKWTGYHVI 491

Query: 486 TSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
             ASEASKFTVGNFIAGGSWLP+T VP+TSGL
Sbjct: 492 -GASEASKFTVGNFIAGGSWLPATNVPYTSGL 518

[Close]
BLAST of Cucsa.257480 vs. NCBI nr
Match: gi|1021482155|ref|XP_016184134.1| (PREDICTED: pectinesterase 2-like [Arachis ipaensis])

HSP 1 Score: 698.4 bits (1801), Expect = 9.6e-198
Identity = 343/509 (67.39%), Postives = 411/509 (80.75%), Query Frame = 1 

Query: 10  VVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHFFEILVE 69
           V+ +  +F S    YS K+IK+WCSQTPYP PCE +L   A  K  PI  KS F ++ ++
Sbjct: 12  VISVPFLFCSIAYGYSLKDIKSWCSQTPYPEPCEYYLSNHAYNK--PIKNKSDFLKVSLQ 71

Query: 70  TALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRT---SARCSPADAQT 129
            A+ERA   H+N +SLGPKCRN+ EK AW DC+ LY+  I +LN+T     +C   DAQT
Sbjct: 72  LAIERAQKGHENTISLGPKCRNAHEKAAWNDCIKLYEYTINKLNKTMDPKTKCDQVDAQT 131

Query: 130 WLSAALTALETCRTGFEELGLSAFGYPLTANN-VSKLISDGLSVNKPASPEGYEPTTMTD 189
           WLS ALT LETC+ GF +LG+  +  PL +NN VS L+S+ LS+NK A    ++P +  D
Sbjct: 132 WLSTALTNLETCKAGFYDLGVQDYMLPLLSNNNVSCLLSNTLSLNKVA----FQPPSYKD 191

Query: 190 GFPTWVSPGNRKLLQSESP--KADVVVAQDGSGNFKTVKDAISAA-KGGGRFVIYIKSGV 249
           GFPTWV PG+RKLLQS S   KA+VVVA+DGSG + TV  A++AA  G  R+VIY+K+GV
Sbjct: 192 GFPTWVKPGDRKLLQSSSAASKANVVVAKDGSGKYTTVSAAVNAAPSGSSRYVIYVKAGV 251

Query: 250 YNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTA 309
           YNE + +KAKNVM+VGDGIGKT+ITGS+SVGGGSTTF SATVA+ GDGFIA+DITFRNTA
Sbjct: 252 YNEQVQVKAKNVMLVGDGIGKTVITGSKSVGGGSTTFSSATVAITGDGFIAQDITFRNTA 311

Query: 310 GAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVL 369
           GA NHQAVALRSGSDLSVFYRCGFEGYQDTLYVY++RQFY++CDIYGTVDFIFGNAA VL
Sbjct: 312 GAANHQAVALRSGSDLSVFYRCGFEGYQDTLYVYSDRQFYRECDIYGTVDFIFGNAATVL 371

Query: 370 QDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGL--SGVKAYLGRPWRQY 429
           Q+CNI AR+PP KTIT+TAQGR+DPNQNTGI IHN +IT++ GL  S VK+YLGRPW+QY
Sbjct: 372 QNCNIYARNPPQKTITVTAQGRTDPNQNTGIIIHNSKITAASGLNPSSVKSYLGRPWKQY 431

Query: 430 SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA 489
           SRTVVMK+ +   I+PAGWM WSGNFALNTLYYAEY NTGPG+ST+NRV+WKGYHV+TSA
Sbjct: 432 SRTVVMKTYLDSLINPAGWMEWSGNFALNTLYYAEYANTGPGSSTSNRVSWKGYHVLTSA 491

Query: 490 SEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           S+AS FTVGNF+AG SWLPSTGVPFTSGL
Sbjct: 492 SQASPFTVGNFLAGNSWLPSTGVPFTSGL 514

[Close]
BLAST of Cucsa.257480 vs. NCBI nr
Match: gi|920718382|gb|KOM57579.1| (hypothetical protein LR48_Vigan11g061200 [Vigna angularis])

HSP 1 Score: 689.5 bits (1778), Expect = 4.4e-195
Identity = 334/513 (65.11%), Postives = 412/513 (80.31%), Query Frame = 1 

Query: 5   TIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHFF 64
           +++  ++LI L+F S +  YS K+I++WC+QTPYP PCE +L   A  K  PI +KS F 
Sbjct: 14  SLLISLLLIPLLFSSVVFSYSLKDIQSWCNQTPYPQPCEYYLTNHAFNK--PIKSKSDFL 73

Query: 65  EILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTS---ARCSP 124
           ++ ++ ALERA     N  S+GPKCRN  EK+AW DC+ LY   I RLN+T     +C+ 
Sbjct: 74  KVSLQLALERAERGQLNTHSIGPKCRNDHEKSAWNDCIQLYQYTIQRLNKTLDPYTKCTQ 133

Query: 125 ADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 184
            D QTWLS ALT LETCR GF ELG++ +  PL +NNV+KL+S+ LS+NK  + + ++P 
Sbjct: 134 TDTQTWLSTALTNLETCRIGFYELGVTDYILPLLSNNVTKLLSNTLSLNKDPNSQ-FKPP 193

Query: 185 TMTDGFPTWVSPGNRKLLQSESP--KADVVVAQDGSGNFKTVKDAISAA-KGGGRFVIYI 244
           +  +GFPTWV PG+RKLLQS S   KA+V+VA+DGSG + TVK A+ AA    GR+VIY+
Sbjct: 194 STQEGFPTWVKPGDRKLLQSSSAASKANVIVAKDGSGKYTTVKAAVDAAPSSSGRYVIYV 253

Query: 245 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 304
           KSGVYNE +++K+KN+M+VGDGIGKTIITGS+SVGGGSTTF SATVA  GDGFIA+DITF
Sbjct: 254 KSGVYNEQVEVKSKNIMLVGDGIGKTIITGSKSVGGGSTTFNSATVAAVGDGFIAQDITF 313

Query: 305 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 364
           RNTAGA NHQAVALRSGSDLSVFYRC FEGYQDTLYV++ERQFY++CDIYGTVDFIFGNA
Sbjct: 314 RNTAGAANHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSERQFYRECDIYGTVDFIFGNA 373

Query: 365 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGL--SGVKAYLGRP 424
           AVVLQ+CNI AR PP KTIT+TAQGR+DPNQNTGI IHN ++T + G   S VK+YLGRP
Sbjct: 374 AVVLQNCNIYARTPPQKTITVTAQGRTDPNQNTGIIIHNSKVTGASGFNPSSVKSYLGRP 433

Query: 425 WRQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHV 484
           W+QYSRTV MK+++   I+PAGWM W GNFAL+TLYYAEY NTGPG+STANRV WKGYH+
Sbjct: 434 WKQYSRTVFMKTNLDSLINPAGWMEWDGNFALDTLYYAEYANTGPGSSTANRVTWKGYHI 493

Query: 485 ITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           +T+AS+A+ FTVGNFIAG SW+P+TGVPFTSGL
Sbjct: 494 LTTASQATPFTVGNFIAGNSWIPNTGVPFTSGL 523

The following BLAST results are available for this feature:
BLAST of Cucsa.257480 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PME2_CITSI5.6e-19063.55Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1[more]
PME17_ARATH2.9e-18662.84Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=P... [more]
PME59_ARATH7.2e-13748.88Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=P... [more]
PME60_ARATH6.7e-13551.58Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana GN=P... [more]
PME33_ARATH1.7e-13049.21Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana GN=P... [more]
BLAST of Cucsa.257480 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L7N4_CUCSA1.3e-299100.00Pectinesterase OS=Cucumis sativus GN=Csa_3G126810 PE=4 SV=1[more]
W9R8N4_9ROSA6.7e-19866.80Pectinesterase OS=Morus notabilis GN=L484_025594 PE=4 SV=1[more]
A0A0S3SY54_PHAAN3.1e-19565.11Pectinesterase OS=Vigna angularis var. angularis GN=Vigan.09G122000 PE=4 SV=1[more]
A0A0L9VS61_PHAAN3.1e-19565.11Pectinesterase OS=Phaseolus angularis GN=LR48_Vigan11g061200 PE=4 SV=1[more]
I1JKR9_SOYBN3.4e-19465.10Pectinesterase OS=Glycine max GN=GLYMA_03G029200 PE=4 SV=1[more]
BLAST of Cucsa.257480 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G45220.11.6e-18762.84 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G51490.14.1e-13848.88 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G51500.13.8e-13651.58 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G47400.19.7e-13249.21 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G00190.16.9e-13049.90 pectin methylesterase 38[more]
BLAST of Cucsa.257480 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449432283|ref|XP_004133929.1|1.9e-299100.00PREDICTED: pectinesterase 2 [Cucumis sativus][more]
gi|659075527|ref|XP_008438193.1|1.6e-28594.91PREDICTED: pectinesterase 2 [Cucumis melo][more]
gi|703108167|ref|XP_010098938.1|9.6e-19866.80Pectinesterase 2 [Morus notabilis][more]
gi|1021482155|ref|XP_016184134.1|9.6e-19867.39PREDICTED: pectinesterase 2-like [Arachis ipaensis][more]
gi|920718382|gb|KOM57579.1|4.4e-19565.11hypothetical protein LR48_Vigan11g061200 [Vigna angularis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR006501Pectinesterase_inhib_dom
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
IPR018040Pectinesterase_Tyr_AS
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
GO:0004857enzyme inhibitor activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.257480.1Cucsa.257480.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 207..494
score: 1.5E
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 26..170
score: 7.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 28..166
score: 1.4
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 24..169
score: 2.0
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 3..149
score: 2.5
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 27..170
score: 1.96
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 204..508
score: 7.18E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 204..508
score: 3.1E
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 230..249
scor
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 1..509
score:
NoneNo IPR availablePANTHERPTHR31707:SF11PECTINESTERASE/PECTINESTERASE INHIBITOR 17-RELATEDcoord: 1..509
score:

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.257480Cla021325Watermelon (97103) v1cgywmB479
Cucsa.257480Csa3G126810Cucumber (Chinese Long) v2cgycuB366
Cucsa.257480MELO3C006208Melon (DHL92) v3.5.1cgymeB439
Cucsa.257480ClCG05G002440Watermelon (Charleston Gray)cgywcgB451
Cucsa.257480CSPI03G09730Wild cucumber (PI 183967)cgycpiB383
Cucsa.257480CmaCh14G019670Cucurbita maxima (Rimu)cgycmaB0709
Cucsa.257480CmaCh06G011470Cucurbita maxima (Rimu)cgycmaB0727
Cucsa.257480CmoCh14G020280Cucurbita moschata (Rifu)cgycmoB0700
Cucsa.257480CmoCh06G011760Cucurbita moschata (Rifu)cgycmoB0718
Cucsa.257480Lsi05G019420Bottle gourd (USVL1VR-Ls)cgylsiB415
Cucsa.257480Cp4.1LG03g17300Cucurbita pepo (Zucchini)cgycpeB0682
Cucsa.257480Cp4.1LG08g02020Cucurbita pepo (Zucchini)cgycpeB0687
Cucsa.257480MELO3C006208.2Melon (DHL92) v3.6.1cgymedB432
Cucsa.257480CsaV3_3G009870Cucumber (Chinese Long) v3cgycucB387
Cucsa.257480Cla97C05G082940Watermelon (97103) v2cgywmbB452
Cucsa.257480BhiUN737G9Wax gourdcgywgoB513
Cucsa.257480Carg14849Silver-seed gourdcarcgyB0693
Cucsa.257480Carg06369Silver-seed gourdcarcgyB1077
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.257480Melon (DHL92) v3.6.1cgymedB423
Cucsa.257480Silver-seed gourdcarcgyB0269
Cucsa.257480Silver-seed gourdcarcgyB1191
Cucsa.257480Cucumber (Chinese Long) v3cgycucB398
Cucsa.257480Watermelon (97103) v2cgywmbB455
Cucsa.257480Wax gourdcgywgoB506
Cucsa.257480Cucumber (Gy14) v1cgycgyB130
Cucsa.257480Cucurbita maxima (Rimu)cgycmaB0715
Cucsa.257480Cucurbita maxima (Rimu)cgycmaB0730
Cucsa.257480Cucurbita moschata (Rifu)cgycmoB0708
Cucsa.257480Cucurbita moschata (Rifu)cgycmoB0719
Cucsa.257480Wild cucumber (PI 183967)cgycpiB393
Cucsa.257480Cucumber (Chinese Long) v2cgycuB374
Cucsa.257480Melon (DHL92) v3.5.1cgymeB429
Cucsa.257480Watermelon (Charleston Gray)cgywcgB454
Cucsa.257480Watermelon (97103) v1cgywmB484
Cucsa.257480Cucurbita pepo (Zucchini)cgycpeB0670
Cucsa.257480Cucurbita pepo (Zucchini)cgycpeB0677
Cucsa.257480Cucurbita pepo (Zucchini)cgycpeB0679
Cucsa.257480Bottle gourd (USVL1VR-Ls)cgylsiB410