Cp4.1LG03g17300 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG03g17300
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectinesterase
LocationCp4.1LG03 : 12742450 .. 12743349 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTGATTCATAGCCGTTGGATCCTTATTGTTCTTGTTGTTCTTAATTTTCCGGCCGCCATTTTTAGTTATTCCGAAAAAGAATTAAAAAATTGGTGTAGCCAAACACCATATCCAGCTCCATGCGAGCAATTTATCAAAGCCAAAGCCACCGCCAAACATTCCCCAATCGCCACTAAATCCCATTTCCTTAAACTCTTGGTCGAAACGGCACTCGACGGCGCCGTTTCGGCCCAAAAAACCGCGCTGTCTCTCGGCCCAAAATGCCACAATGGCCGAGAAAAATCAGCATTGACCGACTGCGTTGACCTCTACAATCAAATCATTTCCCGTCTCAACCGCTCCGCCGACCGCTGCTCCTCCGTCGACGCCCAGACTTGGCTCAGCGCCTCCCTCACCGCCCTCGACACCTGCCGGAACAGCCTCCAGGAGCTCGGGGTTTCGGCCTTCGGCTACCCATTGACGAGCAATAATGTTTCCAAGCTCATTAGCGATGGGTTGTCTGTGAATAAGCCAACGTCACCCCAGGGGTTTGAGCCAACTATGACGGAGGGGTTTCCGACGTGGGTTAGGCCCGGTGACCGGAAACTGCTGCAGTCGGGGTCTCCGAGTGCGGACGTGGTGGTGGCGAAGGACGGGTCTGGGAATTTTAAGACGGTGAAGGAGGCGGTCGCCGCTGCGAAAGGCGGCGGGCGATTTGTAATATATGTGAAGGCGGGGGTTTATAGTGAAAACCTTGAGATTAATGCTAAGAATCTTATGTTGATCGGCGACGGAATCGGAAAAACTGTCATCACCGGAAGTAGAAGTGTCGGCGGCGGTTTCACCACGTTTAGATCCGCCACCGTTGGTATGTCTCTATCTCTCCTTATAAGCTTTTCTAAAATCTTTTTATAA

mRNA sequence

ATGGCAGTGATTCATAGCCGTTGGATCCTTATTGTTCTTGTTGTTCTTAATTTTCCGGCCGCCATTTTTAGTTATTCCGAAAAAGAATTAAAAAATTGGTGTAGCCAAACACCATATCCAGCTCCATGCGAGCAATTTATCAAAGCCAAAGCCACCGCCAAACATTCCCCAATCGCCACTAAATCCCATTTCCTTAAACTCTTGGTCGAAACGGCACTCGACGGCGCCGTTTCGGCCCAAAAAACCGCGCTGTCTCTCGGCCCAAAATGCCACAATGGCCGAGAAAAATCAGCATTGACCGACTGCGTTGACCTCTACAATCAAATCATTTCCCGTCTCAACCGCTCCGCCGACCGCTGCTCCTCCGTCGACGCCCAGACTTGGCTCAGCGCCTCCCTCACCGCCCTCGACACCTGCCGGAACAGCCTCCAGGAGCTCGGGGTTTCGGCCTTCGGCTACCCATTGACGAGCAATAATGTTTCCAAGCTCATTAGCGATGGGTTGTCTGTGAATAAGCCAACGTCACCCCAGGGGTTTGAGCCAACTATGACGGAGGGGTTTCCGACGTGGGTTAGGCCCGGTGACCGGAAACTGCTGCAGTCGGGGTCTCCGAGTGCGGACGTGGTGGTGGCGAAGGACGGGTCTGGGAATTTTAAGACGGTGAAGGAGGCGGTCGCCGCTGCGAAAGGCGGCGGGCGATTTGTAATATATGTGAAGGCGGGGGTTTATAGTGAAAACCTTGAGATTAATGCTAAGAATCTTATGTTGATCGGCGACGGAATCGGAAAAACTGTCATCACCGGAAGTAGAAGTGTCGGCGGCGGTTTCACCACGTTTAGATCCGCCACCGTTGGTATGTCTCTATCTCTCCTTATAAGCTTTTCTAAAATCTTTTTATAA

Coding sequence (CDS)

ATGGCAGTGATTCATAGCCGTTGGATCCTTATTGTTCTTGTTGTTCTTAATTTTCCGGCCGCCATTTTTAGTTATTCCGAAAAAGAATTAAAAAATTGGTGTAGCCAAACACCATATCCAGCTCCATGCGAGCAATTTATCAAAGCCAAAGCCACCGCCAAACATTCCCCAATCGCCACTAAATCCCATTTCCTTAAACTCTTGGTCGAAACGGCACTCGACGGCGCCGTTTCGGCCCAAAAAACCGCGCTGTCTCTCGGCCCAAAATGCCACAATGGCCGAGAAAAATCAGCATTGACCGACTGCGTTGACCTCTACAATCAAATCATTTCCCGTCTCAACCGCTCCGCCGACCGCTGCTCCTCCGTCGACGCCCAGACTTGGCTCAGCGCCTCCCTCACCGCCCTCGACACCTGCCGGAACAGCCTCCAGGAGCTCGGGGTTTCGGCCTTCGGCTACCCATTGACGAGCAATAATGTTTCCAAGCTCATTAGCGATGGGTTGTCTGTGAATAAGCCAACGTCACCCCAGGGGTTTGAGCCAACTATGACGGAGGGGTTTCCGACGTGGGTTAGGCCCGGTGACCGGAAACTGCTGCAGTCGGGGTCTCCGAGTGCGGACGTGGTGGTGGCGAAGGACGGGTCTGGGAATTTTAAGACGGTGAAGGAGGCGGTCGCCGCTGCGAAAGGCGGCGGGCGATTTGTAATATATGTGAAGGCGGGGGTTTATAGTGAAAACCTTGAGATTAATGCTAAGAATCTTATGTTGATCGGCGACGGAATCGGAAAAACTGTCATCACCGGAAGTAGAAGTGTCGGCGGCGGTTTCACCACGTTTAGATCCGCCACCGTTGGTATGTCTCTATCTCTCCTTATAAGCTTTTCTAAAATCTTTTTATAA

Protein sequence

MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRCSSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGMSLSLLISFSKIFL
Homology
[Close]
BLAST of Cp4.1LG03g17300 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 296.6 bits (758), Expect = 3.1e-79
Identity = 153/279 (54.84%), Postives = 200/279 (71.68%), Query Frame = 1 

Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLL 68
           I  V++ +   + +  Y++K++K WCSQTP P PCE F+   +   + PI ++S FLK+ 
Sbjct: 9   INFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSN--NEPIKSESEFLKIS 68

Query: 69  VETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD---RCSSVDA 128
           ++  LD A+ A+  A +LGPKC + REK+A  DC+ LY+  +S++N + D   +CS +DA
Sbjct: 69  MKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDA 128

Query: 129 QTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTE 188
           QTWLS +LT LDTCR    ELGV+    PL SNNVS L+ + L++NK   P  + P   +
Sbjct: 129 QTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKV--PFNYTPPEKD 188

Query: 189 GFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSE 248
           GFP+WV+PGDRKLLQS +P  + VVAKDGSGNFKT+KEA+ AA G GRFVIYVK GVYSE
Sbjct: 189 GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGVYSE 248

Query: 249 NLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATV 285
           NLEI  KN+ML GDGIGKT+ITGS+SVGGG TTF SATV
Sbjct: 249 NLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 283

[Close]
BLAST of Cp4.1LG03g17300 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.1e-73
Identity = 148/283 (52.30%), Postives = 202/283 (71.38%), Query Frame = 1 

Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLL 68
           I + LV+ +     F YS +E+K+WC +TP P PCE F+  K     + I   + F K+ 
Sbjct: 7   ITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDV--TSIKQDTDFYKIS 66

Query: 69  VETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADR---CSSVDA 128
           ++ AL+ A +AQ    +LG KC N REK+A  DC +LY   + +LN++++    C+ VD 
Sbjct: 67  LQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDK 126

Query: 129 QTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTE 188
           QTWLS +LT L+TCR SL++LGV  +  PL SNNV+KLIS+ LS+NK   P   EP+  +
Sbjct: 127 QTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNK--VPYN-EPSYKD 186

Query: 189 GFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAK--GGGRFVIYVKAGVY 248
           GFPTWV+PGDRKLLQ+ +P A++VVA+DGSGN KT++EAVAAA   GG R+VIY+KAG Y
Sbjct: 187 GFPTWVKPGDRKLLQT-TPRANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTY 246

Query: 249 SENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           +EN+E+  KN+M +GDGIGKT+ITGS+SVGGG TTF+SATV +
Sbjct: 247 NENIEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAV 283

[Close]
BLAST of Cp4.1LG03g17300 vs. Swiss-Prot
Match: PME60_ARATH (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana GN=PME60 PE=2 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 3.2e-52
Identity = 127/299 (42.47%), Postives = 175/299 (58.53%), Query Frame = 1 

Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATK---SHFL 68
           +LI+L+ L     +   +   +  WC +TPYP PC+++       KHS        S F 
Sbjct: 17  LLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYF-----IKHSGFRLPTQISEFR 76

Query: 69  KLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD------- 128
            LLVE A+D AVSA     +    C + ++++ L DC++LY   + +LNR+         
Sbjct: 77  VLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTG 136

Query: 129 -RCSSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNN-VSKLISDGLSVNKPTSP 188
            RC+  DAQTWLS +LT  +TCR    +L VS F  P+ SN  +S LIS+ L+VN     
Sbjct: 137 RRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLT 196

Query: 189 QGFEPTMT---EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAA----K 248
            G   + T   +GFPTWV   +R+LLQ  S  A++VVAKDGSG+FKTV+ A+  A     
Sbjct: 197 AGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKV 256

Query: 249 GGGRFVIYVKAGVYSENLEI--NAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
             GRFVIYVK G+Y ENL +  N  N+ML+GDG+  T+ITG RSV GG+TT+ SAT G+
Sbjct: 257 TSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGI 310

[Close]
BLAST of Cp4.1LG03g17300 vs. Swiss-Prot
Match: PME59_ARATH (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=PME59 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.9e-49
Identity = 118/296 (39.86%), Postives = 175/296 (59.12%), Query Frame = 1 

Query: 11  IVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLLVE 70
           ++L+VL     + + +++   +WC +TPYP PC+ + K     +       S F  +LVE
Sbjct: 15  LILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQP--TQLSEFRVMLVE 74

Query: 71  TALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRS----------ADRC 130
            A+D A+SA+    + G  C + ++++ L DC+DLY   I +LNR+          A  C
Sbjct: 75  AAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSC 134

Query: 131 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSN-NVSKLISDGLSVNKPTSPQGF 190
           +  DAQTWLS +LT  +TCR    +L V+ F  P+ SN  +S LIS+ L+VN      G 
Sbjct: 135 TDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGALLTAGN 194

Query: 191 EPTMT---EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAA----KGGG 250
           +   T   +GFPTW+   D++LL+  +  A++VVAKDGSG+F TV+ A+  A       G
Sbjct: 195 KGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVAGRRKVTSG 254

Query: 251 RFVIYVKAGVYSENLEINAKN--LMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           RFVIYVK G+Y EN+ +   N  +ML+GDG+  T+ITG RSV GG+TT+ SAT G+
Sbjct: 255 RFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGI 306

[Close]
BLAST of Cp4.1LG03g17300 vs. Swiss-Prot
Match: PME33_ARATH (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana GN=PME33 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 6.1e-43
Identity = 112/291 (38.49%), Postives = 169/291 (58.08%), Query Frame = 1 

Query: 33  WCSQTPYPAPCEQFIKAKATAKHS--PIATKSHFLKLLVETALDGAVSAQKTALSLGPKC 92
           WC++TP+   C  + +  +    +  P   +S FL++LV+ ALD AV      +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 93  HNGREKSALTDCVDLYNQIISRLNR---------SAD-RCSSVDAQTWLSASLTALDTCR 152
            N + K+A +DCV+L+   +++LNR         S+D +C+  DAQTWLS + T ++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 153 NSLQELGVSAFGYPLTSN-NVSKLISDGLSVNKPTSPQGFEPTMT---EGFPTWVRPGDR 212
           +  ++L VS F  P+ SN N+S LI + L+VN     Q    T     E FP+WV   +R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 213 KLLQSGS---PSADVVVAKDGSGNFKTVKEAV---AAAKGGGRFVIYVKAGVYSENLEI- 272
           +LL S S    S  +VVA+D SG+F++++ A+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 273 -NAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGMSLSLLISFSKIFL 300
            +  N+ML+GDG  KT+IT  RSV  G+TT+ SAT G      ++    F+
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFI 377

[Close]
BLAST of Cp4.1LG03g17300 vs. TrEMBL
Match: A0A0A0L7N4_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G126810 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.4e-118
Identity = 211/283 (74.56%), Postives = 250/283 (88.34%), Query Frame = 1 

Query: 5   HSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHF 64
           H+    +VL+VL FP+ I  YSEKE+KNWCSQTPYPAPCE+F+K KAT K +PI TKSHF
Sbjct: 4   HTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHF 63

Query: 65  LKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRCSSVD 124
            ++LVETAL+ AVSA K ALSLGPKC N +EK+A TDCVDLY+QII+RLNR++ RCS  D
Sbjct: 64  FEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPAD 123

Query: 125 AQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEP-TM 184
           AQTWLSA+LTAL+TCR   +ELG+SAFGYPLT+NNVSKLISDGLSVNKP SP+G+EP TM
Sbjct: 124 AQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTM 183

Query: 185 TEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVY 244
           T+GFPTWV PG+RKLLQS SP ADVVVA+DGSGNFKTVK+A++AAKGGGRFVIY+K+GVY
Sbjct: 184 TDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVY 243

Query: 245 SENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           +ENL+I AKN+M++GDGIGKT+ITGSRSVGGG TTFRSATV +
Sbjct: 244 NENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAV 286

[Close]
BLAST of Cp4.1LG03g17300 vs. TrEMBL
Match: A0A087H5B3_ARAAL (Pectinesterase OS=Arabis alpina GN=AALP_AA4G241100 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.6e-77
Identity = 153/266 (57.52%), Postives = 194/266 (72.93%), Query Frame = 1 

Query: 22  IFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLLVETALDGAVSAQK 81
           +  Y  K++K WCSQTP P PCE F+   +  K  PI T+S FLKL ++ AL+ A+ A+ 
Sbjct: 20  VSGYKSKDVKAWCSQTPNPIPCEYFLTHNSNNK--PIKTESEFLKLSMKLALERAILAKN 79

Query: 82  TALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD---RCSSVDAQTWLSASLTALDT 141
            A +LGPKC + REK+A  DC++LY+  +S++N + D   +CS  DAQTWLS +LT LDT
Sbjct: 80  HAFTLGPKCRDKREKAAWEDCLNLYDFTVSKINATMDTNVKCSKTDAQTWLSTALTNLDT 139

Query: 142 CRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTEGFPTWVRPGDRKL 201
           CR    +LGV+    PL SNNVSKL+ + L++NK   P  + P   +GFP+WV+PGDRKL
Sbjct: 140 CRAGFLDLGVTDIVLPLMSNNVSKLLCNTLAINK--VPFNYTPPEKDGFPSWVKPGDRKL 199

Query: 202 LQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSENLEINAKNLMLIG 261
           LQS +PS + VVAKDGSG FKT+K+A+ AA G GRFVIYVK GVY+ENLEI  KN+ML G
Sbjct: 200 LQSSTPSDNAVVAKDGSGTFKTIKDAIDAASGSGRFVIYVKQGVYAENLEIRKKNIMLRG 259

Query: 262 DGIGKTVITGSRSVGGGFTTFRSATV 285
           DGIGKT+ITGSRSVGGG TTF SATV
Sbjct: 260 DGIGKTIITGSRSVGGGTTTFNSATV 281

[Close]
BLAST of Cp4.1LG03g17300 vs. TrEMBL
Match: D7LCG8_ARALL (Pectinesterase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_670486 PE=4 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 5.8e-77
Identity = 154/279 (55.20%), Postives = 199/279 (71.33%), Query Frame = 1 

Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLL 68
           I  V + +   + +  Y++K++K WCSQTP P PCE F+   +  K  PI ++S FL++ 
Sbjct: 9   ITFVFLCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNK--PIKSESEFLEIS 68

Query: 69  VETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD---RCSSVDA 128
           ++ ALD AV A+  A +LGPKC + REK+A  DC+ LY+  +S++N + D   +CS  DA
Sbjct: 69  MKLALDRAVLAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKTDA 128

Query: 129 QTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTE 188
           QTWLS +LT LDTCR    ELGV+    PL SNNVS L+ + L++NK   P  + P   +
Sbjct: 129 QTWLSTALTNLDTCRAGFLELGVTDVVLPLMSNNVSNLLCNTLAINK--VPFNYTPPEKD 188

Query: 189 GFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSE 248
           GFP+WV+PGDRKLLQS +P  + VVAKDGSGNFKT+K+A+ AA G GRFVIYVK GVYSE
Sbjct: 189 GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKDAINAASGSGRFVIYVKQGVYSE 248

Query: 249 NLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATV 285
           NLEI  KN+ML GDGIGKT+ITGS+SVGGG TTF SATV
Sbjct: 249 NLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 283

[Close]
BLAST of Cp4.1LG03g17300 vs. TrEMBL
Match: A0A0S3SY54_PHAAN (Pectinesterase OS=Vigna angularis var. angularis GN=Vigan.09G122000 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.7e-76
Identity = 155/282 (54.96%), Postives = 203/282 (71.99%), Query Frame = 1 

Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLL 68
           I ++L+ L F + +FSYS K++++WC+QTPYP PCE ++   A  K  PI +KS FLK+ 
Sbjct: 17  ISLLLIPLLFSSVVFSYSLKDIQSWCNQTPYPQPCEYYLTNHAFNK--PIKSKSDFLKVS 76

Query: 69  VETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD---RCSSVDA 128
           ++ AL+ A   Q    S+GPKC N  EKSA  DC+ LY   I RLN++ D   +C+  D 
Sbjct: 77  LQLALERAERGQLNTHSIGPKCRNDHEKSAWNDCIQLYQYTIQRLNKTLDPYTKCTQTDT 136

Query: 129 QTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTE 188
           QTWLS +LT L+TCR    ELGV+ +  PL SNNV+KL+S+ LS+NK  + Q   P+  E
Sbjct: 137 QTWLSTALTNLETCRIGFYELGVTDYILPLLSNNVTKLLSNTLSLNKDPNSQFKPPSTQE 196

Query: 189 GFPTWVRPGDRKLLQSGSPS--ADVVVAKDGSGNFKTVKEAV-AAAKGGGRFVIYVKAGV 248
           GFPTWV+PGDRKLLQS S +  A+V+VAKDGSG + TVK AV AA    GR+VIYVK+GV
Sbjct: 197 GFPTWVKPGDRKLLQSSSAASKANVIVAKDGSGKYTTVKAAVDAAPSSSGRYVIYVKSGV 256

Query: 249 YSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATV 285
           Y+E +E+ +KN+ML+GDGIGKT+ITGS+SVGGG TTF SATV
Sbjct: 257 YNEQVEVKSKNIMLVGDGIGKTIITGSKSVGGGSTTFNSATV 296

[Close]
BLAST of Cp4.1LG03g17300 vs. TrEMBL
Match: A0A0L9VS61_PHAAN (Pectinesterase OS=Phaseolus angularis GN=LR48_Vigan11g061200 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.7e-76
Identity = 155/282 (54.96%), Postives = 203/282 (71.99%), Query Frame = 1 

Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLL 68
           I ++L+ L F + +FSYS K++++WC+QTPYP PCE ++   A  K  PI +KS FLK+ 
Sbjct: 17  ISLLLIPLLFSSVVFSYSLKDIQSWCNQTPYPQPCEYYLTNHAFNK--PIKSKSDFLKVS 76

Query: 69  VETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD---RCSSVDA 128
           ++ AL+ A   Q    S+GPKC N  EKSA  DC+ LY   I RLN++ D   +C+  D 
Sbjct: 77  LQLALERAERGQLNTHSIGPKCRNDHEKSAWNDCIQLYQYTIQRLNKTLDPYTKCTQTDT 136

Query: 129 QTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTE 188
           QTWLS +LT L+TCR    ELGV+ +  PL SNNV+KL+S+ LS+NK  + Q   P+  E
Sbjct: 137 QTWLSTALTNLETCRIGFYELGVTDYILPLLSNNVTKLLSNTLSLNKDPNSQFKPPSTQE 196

Query: 189 GFPTWVRPGDRKLLQSGSPS--ADVVVAKDGSGNFKTVKEAV-AAAKGGGRFVIYVKAGV 248
           GFPTWV+PGDRKLLQS S +  A+V+VAKDGSG + TVK AV AA    GR+VIYVK+GV
Sbjct: 197 GFPTWVKPGDRKLLQSSSAASKANVIVAKDGSGKYTTVKAAVDAAPSSSGRYVIYVKSGV 256

Query: 249 YSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATV 285
           Y+E +E+ +KN+ML+GDGIGKT+ITGS+SVGGG TTF SATV
Sbjct: 257 YNEQVEVKSKNIMLVGDGIGKTIITGSKSVGGGSTTFNSATV 296

[Close]
BLAST of Cp4.1LG03g17300 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 296.6 bits (758), Expect = 1.7e-80
Identity = 153/279 (54.84%), Postives = 200/279 (71.68%), Query Frame = 1 

Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLL 68
           I  V++ +   + +  Y++K++K WCSQTP P PCE F+   +   + PI ++S FLK+ 
Sbjct: 9   INFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSN--NEPIKSESEFLKIS 68

Query: 69  VETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD---RCSSVDA 128
           ++  LD A+ A+  A +LGPKC + REK+A  DC+ LY+  +S++N + D   +CS +DA
Sbjct: 69  MKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDA 128

Query: 129 QTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTE 188
           QTWLS +LT LDTCR    ELGV+    PL SNNVS L+ + L++NK   P  + P   +
Sbjct: 129 QTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKV--PFNYTPPEKD 188

Query: 189 GFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSE 248
           GFP+WV+PGDRKLLQS +P  + VVAKDGSGNFKT+KEA+ AA G GRFVIYVK GVYSE
Sbjct: 189 GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGVYSE 248

Query: 249 NLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATV 285
           NLEI  KN+ML GDGIGKT+ITGS+SVGGG TTF SATV
Sbjct: 249 NLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 283

[Close]
BLAST of Cp4.1LG03g17300 vs. TAIR10
Match: AT5G51500.1 (AT5G51500.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 206.8 bits (525), Expect = 1.8e-53
Identity = 127/299 (42.47%), Postives = 175/299 (58.53%), Query Frame = 1 

Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATK---SHFL 68
           +LI+L+ L     +   +   +  WC +TPYP PC+++       KHS        S F 
Sbjct: 17  LLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYF-----IKHSGFRLPTQISEFR 76

Query: 69  KLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD------- 128
            LLVE A+D AVSA     +    C + ++++ L DC++LY   + +LNR+         
Sbjct: 77  VLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTG 136

Query: 129 -RCSSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNN-VSKLISDGLSVNKPTSP 188
            RC+  DAQTWLS +LT  +TCR    +L VS F  P+ SN  +S LIS+ L+VN     
Sbjct: 137 RRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLT 196

Query: 189 QGFEPTMT---EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAA----K 248
            G   + T   +GFPTWV   +R+LLQ  S  A++VVAKDGSG+FKTV+ A+  A     
Sbjct: 197 AGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKV 256

Query: 249 GGGRFVIYVKAGVYSENLEI--NAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
             GRFVIYVK G+Y ENL +  N  N+ML+GDG+  T+ITG RSV GG+TT+ SAT G+
Sbjct: 257 TSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGI 310

[Close]
BLAST of Cp4.1LG03g17300 vs. TAIR10
Match: AT5G51490.1 (AT5G51490.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 197.6 bits (501), Expect = 1.1e-50
Identity = 118/296 (39.86%), Postives = 175/296 (59.12%), Query Frame = 1 

Query: 11  IVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLLVE 70
           ++L+VL     + + +++   +WC +TPYP PC+ + K     +       S F  +LVE
Sbjct: 15  LILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQP--TQLSEFRVMLVE 74

Query: 71  TALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRS----------ADRC 130
            A+D A+SA+    + G  C + ++++ L DC+DLY   I +LNR+          A  C
Sbjct: 75  AAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSC 134

Query: 131 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSN-NVSKLISDGLSVNKPTSPQGF 190
           +  DAQTWLS +LT  +TCR    +L V+ F  P+ SN  +S LIS+ L+VN      G 
Sbjct: 135 TDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGALLTAGN 194

Query: 191 EPTMT---EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAA----KGGG 250
           +   T   +GFPTW+   D++LL+  +  A++VVAKDGSG+F TV+ A+  A       G
Sbjct: 195 KGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVAGRRKVTSG 254

Query: 251 RFVIYVKAGVYSENLEINAKN--LMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           RFVIYVK G+Y EN+ +   N  +ML+GDG+  T+ITG RSV GG+TT+ SAT G+
Sbjct: 255 RFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGI 306

[Close]
BLAST of Cp4.1LG03g17300 vs. TAIR10
Match: AT3G47400.1 (AT3G47400.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 176.0 bits (445), Expect = 3.4e-44
Identity = 112/291 (38.49%), Postives = 169/291 (58.08%), Query Frame = 1 

Query: 33  WCSQTPYPAPCEQFIKAKATAKHS--PIATKSHFLKLLVETALDGAVSAQKTALSLGPKC 92
           WC++TP+   C  + +  +    +  P   +S FL++LV+ ALD AV      +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 93  HNGREKSALTDCVDLYNQIISRLNR---------SAD-RCSSVDAQTWLSASLTALDTCR 152
            N + K+A +DCV+L+   +++LNR         S+D +C+  DAQTWLS + T ++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 153 NSLQELGVSAFGYPLTSN-NVSKLISDGLSVNKPTSPQGFEPTMT---EGFPTWVRPGDR 212
           +  ++L VS F  P+ SN N+S LI + L+VN     Q    T     E FP+WV   +R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 213 KLLQSGS---PSADVVVAKDGSGNFKTVKEAV---AAAKGGGRFVIYVKAGVYSENLEI- 272
           +LL S S    S  +VVA+D SG+F++++ A+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 273 -NAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGMSLSLLISFSKIFL 300
            +  N+ML+GDG  KT+IT  RSV  G+TT+ SAT G      ++    F+
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFI 377

[Close]
BLAST of Cp4.1LG03g17300 vs. TAIR10
Match: AT4G00190.1 (AT4G00190.1 pectin methylesterase 38)

HSP 1 Score: 165.2 bits (417), Expect = 6.0e-41
Identity = 107/260 (41.15%), Postives = 153/260 (58.85%), Query Frame = 1 

Query: 34  CSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLLVETALDGAVSAQKTALSLGPKCHNG 93
           C +TP+P  C+  +      KH PI + + FL++ VE  LDGAV A+     L P+  + 
Sbjct: 8   CDETPHPGECKTLL-----IKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS- 67

Query: 94  REKSALTDCVDLYNQIISRLNRSA----DRCSSVDAQTWLSASLTALDTCRNSLQELGVS 153
             K A  +C+DLY Q I RLN S     + CS  D Q WLS +LT LDTC+  + ELGVS
Sbjct: 68  --KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELGVS 127

Query: 154 AFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTEGFPTWVRPGDRKLLQSGSPSADVV 213
           +  + L S  +   + + L++NK     G E  +++     +  G++          DVV
Sbjct: 128 S--HSLESITID--VINTLAINKRMEQNGKEFGISKITMKTLSIGEK---------VDVV 187

Query: 214 VAKDGSGNFKTVKEAVAAA----KGGGRFVIYVKAGVYSE--NLEINAKNLMLIGDGIGK 273
           VA+DGSG++KT++EAV  A    KG  R+VI+VK GVY E  N+ I + N+M+ GDGIGK
Sbjct: 188 VAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMITGDGIGK 246

Query: 274 TVITGSRSVGGGFTTFRSAT 284
           T+ITG +S G GF+T++SAT
Sbjct: 248 TIITGDKSKGRGFSTYKSAT 246

[Close]
BLAST of Cp4.1LG03g17300 vs. NCBI nr
Match: gi|659075527|ref|XP_008438193.1| (PREDICTED: pectinesterase 2 [Cucumis melo])

HSP 1 Score: 436.0 bits (1120), Expect = 5.2e-119
Identity = 217/285 (76.14%), Postives = 251/285 (88.07%), Query Frame = 1 

Query: 5   HSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHS-PIATKSH 64
           HS   L+VLVVL FP  +  YSEKE+KNWCSQTPYPAPCE+F+K KA+ K S PI TKSH
Sbjct: 4   HSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKSH 63

Query: 65  FLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRCSSV 124
           F ++LVETAL+ AVSA K ALSLGPKC N +EK+A TDCVDLY++II+RLNR++DRC+  
Sbjct: 64  FFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTPA 123

Query: 125 DAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPT- 184
           DAQTWLSA+LTAL+TCR    ELG+SAFGYPLT+NNVSKLISDGLSVNKP SP+G+EPT 
Sbjct: 124 DAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTT 183

Query: 185 -MTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAG 244
            M +GFPTWVRPGDRKLLQSGSP A+VVVA+DGSGNFKTVKEAVAAAKGGGRFVIYVK+G
Sbjct: 184 IMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYVKSG 243

Query: 245 VYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           VY+ENL+I AKN+M++GDGIGKT+ITGSRSVGGG TTFRSATV +
Sbjct: 244 VYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAV 288

[Close]
BLAST of Cp4.1LG03g17300 vs. NCBI nr
Match: gi|449432283|ref|XP_004133929.1| (PREDICTED: pectinesterase 2 [Cucumis sativus])

HSP 1 Score: 434.1 bits (1115), Expect = 2.0e-118
Identity = 211/283 (74.56%), Postives = 250/283 (88.34%), Query Frame = 1 

Query: 5   HSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHF 64
           H+    +VL+VL FP+ I  YSEKE+KNWCSQTPYPAPCE+F+K KAT K +PI TKSHF
Sbjct: 4   HTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHF 63

Query: 65  LKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRCSSVD 124
            ++LVETAL+ AVSA K ALSLGPKC N +EK+A TDCVDLY+QII+RLNR++ RCS  D
Sbjct: 64  FEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPAD 123

Query: 125 AQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEP-TM 184
           AQTWLSA+LTAL+TCR   +ELG+SAFGYPLT+NNVSKLISDGLSVNKP SP+G+EP TM
Sbjct: 124 AQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTM 183

Query: 185 TEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVY 244
           T+GFPTWV PG+RKLLQS SP ADVVVA+DGSGNFKTVK+A++AAKGGGRFVIY+K+GVY
Sbjct: 184 TDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVY 243

Query: 245 SENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           +ENL+I AKN+M++GDGIGKT+ITGSRSVGGG TTFRSATV +
Sbjct: 244 NENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAV 286

[Close]
BLAST of Cp4.1LG03g17300 vs. NCBI nr
Match: gi|729354701|ref|XP_010544633.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 17 [Tarenaya hassleriana])

HSP 1 Score: 307.4 bits (786), Expect = 2.8e-80
Identity = 161/285 (56.49%), Postives = 205/285 (71.93%), Query Frame = 1 

Query: 8   WILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKL 67
           ++ I    +     +F Y +K++K+WCS+TP P PCE F+       H PI ++S FLK+
Sbjct: 6   YVTIFFFCIFLGPTVFGYDQKDVKSWCSKTPNPKPCEYFLTHNPN--HRPIKSESEFLKI 65

Query: 68  LVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD---RCSSVD 127
            ++ ALD A+ A+  A +LGPKC + REK+A  DC+ LY+  +S+LN + D   +CS VD
Sbjct: 66  SMKLALDRALLAKSHAFTLGPKCRDSREKAAWEDCLKLYDYTVSKLNETVDPYVKCSKVD 125

Query: 128 AQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMT 187
           AQTWLS++LT L+TCR    ELGV  F  PL SNNVSKLIS+ L++NK   P  + P+  
Sbjct: 126 AQTWLSSALTNLETCRTGFIELGVPDFLLPLMSNNVSKLISNTLAINK--VPFNYTPSQE 185

Query: 188 EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAA---KGGGRFVIYVKAG 247
            GFP WVRPGDRKLLQS +P A+VVVAKDGSGNF+T+KEAV AA    G GRFVIYVK G
Sbjct: 186 NGFPEWVRPGDRKLLQSSTPQANVVVAKDGSGNFRTIKEAVNAASRRSGSGRFVIYVKQG 245

Query: 248 VYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           VY+EN+EI AKN+ML GDGIGKT+ITGS+SVGGG TTF SATV +
Sbjct: 246 VYNENIEIRAKNIMLRGDGIGKTIITGSKSVGGGSTTFNSATVAV 286

[Close]
BLAST of Cp4.1LG03g17300 vs. NCBI nr
Match: gi|729455378|ref|XP_010524372.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 17 [Tarenaya hassleriana])

HSP 1 Score: 299.7 bits (766), Expect = 5.8e-78
Identity = 157/285 (55.09%), Postives = 204/285 (71.58%), Query Frame = 1 

Query: 8   WILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKL 67
           ++ I L  +     +F++  +++K+WCS+TP P PCE F+       H PI ++S FLK+
Sbjct: 6   YVTIFLFCVFLGPTVFAFDSRDVKSWCSKTPNPTPCEYFLTYDPN--HQPIKSESEFLKI 65

Query: 68  LVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD---RCSSVD 127
            V+ ALD A+ A+  A +LGPKC + REK+A  DC+ LY+  +S+LN + D   +CS VD
Sbjct: 66  SVKLALDRAMLAKNHAFTLGPKCRDSREKTAWEDCLKLYDYTVSKLNETIDPHVKCSKVD 125

Query: 128 AQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMT 187
           AQTWLS++LT L+TCR    ELGV  F  PL SNNVSKLIS+ L++N+   P  + P   
Sbjct: 126 AQTWLSSALTNLETCRTGFIELGVPDFLLPLMSNNVSKLISNTLAINE--VPFNYTPPSE 185

Query: 188 EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAA---KGGGRFVIYVKAG 247
            GFP WVRPGDRKLLQS +P A+ VVA+DGSGNF+T+KEAV +A    G GRFVIYVK G
Sbjct: 186 NGFPEWVRPGDRKLLQSSTPQANAVVAQDGSGNFRTIKEAVDSASKRSGSGRFVIYVKQG 245

Query: 248 VYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           VY+EN+EI AKN+ML GDGIGKT+ITGS+SVGGG TTF SATV +
Sbjct: 246 VYNENIEIKAKNIMLRGDGIGKTIITGSKSVGGGATTFNSATVAV 286

[Close]
BLAST of Cp4.1LG03g17300 vs. NCBI nr
Match: gi|674244550|gb|KFK37315.1| (hypothetical protein AALP_AA4G241100 [Arabis alpina])

HSP 1 Score: 297.0 bits (759), Expect = 3.8e-77
Identity = 153/266 (57.52%), Postives = 194/266 (72.93%), Query Frame = 1 

Query: 22  IFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLLVETALDGAVSAQK 81
           +  Y  K++K WCSQTP P PCE F+   +  K  PI T+S FLKL ++ AL+ A+ A+ 
Sbjct: 20  VSGYKSKDVKAWCSQTPNPIPCEYFLTHNSNNK--PIKTESEFLKLSMKLALERAILAKN 79

Query: 82  TALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD---RCSSVDAQTWLSASLTALDT 141
            A +LGPKC + REK+A  DC++LY+  +S++N + D   +CS  DAQTWLS +LT LDT
Sbjct: 80  HAFTLGPKCRDKREKAAWEDCLNLYDFTVSKINATMDTNVKCSKTDAQTWLSTALTNLDT 139

Query: 142 CRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTEGFPTWVRPGDRKL 201
           CR    +LGV+    PL SNNVSKL+ + L++NK   P  + P   +GFP+WV+PGDRKL
Sbjct: 140 CRAGFLDLGVTDIVLPLMSNNVSKLLCNTLAINK--VPFNYTPPEKDGFPSWVKPGDRKL 199

Query: 202 LQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSENLEINAKNLMLIG 261
           LQS +PS + VVAKDGSG FKT+K+A+ AA G GRFVIYVK GVY+ENLEI  KN+ML G
Sbjct: 200 LQSSTPSDNAVVAKDGSGTFKTIKDAIDAASGSGRFVIYVKQGVYAENLEIRKKNIMLRG 259

Query: 262 DGIGKTVITGSRSVGGGFTTFRSATV 285
           DGIGKT+ITGSRSVGGG TTF SATV
Sbjct: 260 DGIGKTIITGSRSVGGGTTTFNSATV 281

The following BLAST results are available for this feature:
BLAST of Cp4.1LG03g17300 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PME17_ARATH3.1e-7954.84Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=P... [more]
PME2_CITSI1.1e-7352.30Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1[more]
PME60_ARATH3.2e-5242.47Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana GN=P... [more]
PME59_ARATH1.9e-4939.86Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=P... [more]
PME33_ARATH6.1e-4338.49Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana GN=P... [more]
BLAST of Cp4.1LG03g17300 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L7N4_CUCSA1.4e-11874.56Pectinesterase OS=Cucumis sativus GN=Csa_3G126810 PE=4 SV=1[more]
A0A087H5B3_ARAAL2.6e-7757.52Pectinesterase OS=Arabis alpina GN=AALP_AA4G241100 PE=4 SV=1[more]
D7LCG8_ARALL5.8e-7755.20Pectinesterase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_670486 PE=4 SV=... [more]
A0A0S3SY54_PHAAN1.7e-7654.96Pectinesterase OS=Vigna angularis var. angularis GN=Vigan.09G122000 PE=4 SV=1[more]
A0A0L9VS61_PHAAN1.7e-7654.96Pectinesterase OS=Phaseolus angularis GN=LR48_Vigan11g061200 PE=4 SV=1[more]
BLAST of Cp4.1LG03g17300 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G45220.11.7e-8054.84 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G51500.11.8e-5342.47 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G51490.11.1e-5039.86 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G47400.13.4e-4438.49 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G00190.16.0e-4141.15 pectin methylesterase 38[more]
BLAST of Cp4.1LG03g17300 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659075527|ref|XP_008438193.1|5.2e-11976.14PREDICTED: pectinesterase 2 [Cucumis melo][more]
gi|449432283|ref|XP_004133929.1|2.0e-11874.56PREDICTED: pectinesterase 2 [Cucumis sativus][more]
gi|729354701|ref|XP_010544633.1|2.8e-8056.49PREDICTED: probable pectinesterase/pectinesterase inhibitor 17 [Tarenaya hassler... [more]
gi|729455378|ref|XP_010524372.1|5.8e-7855.09PREDICTED: probable pectinesterase/pectinesterase inhibitor 17 [Tarenaya hassler... [more]
gi|674244550|gb|KFK37315.1|3.8e-7757.52hypothetical protein AALP_AA4G241100 [Arabis alpina][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR006501Pectinesterase_inhib_dom
IPR000070Pectinesterase_cat
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g17300.1Cp4.1LG03g17300.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 207..287
score: 7.7
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 27..171
score: 1.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 30..168
score: 9.3
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 25..170
score: 5.9
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 10..159
score: 1.3
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 28..171
score: 2.22
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 204..285
score: 1.13
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 204..285
score: 8.2
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 1..287
score: 7.0E
NoneNo IPR availablePANTHERPTHR31707:SF11PECTINESTERASE/PECTINESTERASE INHIBITOR 17-RELATEDcoord: 1..287
score: 7.0E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG03g17300Cucsa.257480Cucumber (Gy14) v1cgycpeB0682
Cp4.1LG03g17300CmaCh14G019670Cucurbita maxima (Rimu)cmacpeB283
Cp4.1LG03g17300CmaCh06G011470Cucurbita maxima (Rimu)cmacpeB837
Cp4.1LG03g17300CmoCh06G011760Cucurbita moschata (Rifu)cmocpeB787
Cp4.1LG03g17300CmoCh14G020280Cucurbita moschata (Rifu)cmocpeB245
Cp4.1LG03g17300Cla021325Watermelon (97103) v1cpewmB613
Cp4.1LG03g17300Csa3G126810Cucumber (Chinese Long) v2cpecuB609
Cp4.1LG03g17300MELO3C006208Melon (DHL92) v3.5.1cpemeB585
Cp4.1LG03g17300ClCG05G002440Watermelon (Charleston Gray)cpewcgB559
Cp4.1LG03g17300CSPI03G09730Wild cucumber (PI 183967)cpecpiB611
Cp4.1LG03g17300Lsi05G019420Bottle gourd (USVL1VR-Ls)cpelsiB506
Cp4.1LG03g17300MELO3C006208.2Melon (DHL92) v3.6.1cpemedB693
Cp4.1LG03g17300CsaV3_3G009870Cucumber (Chinese Long) v3cpecucB0759
Cp4.1LG03g17300CsGy3G009820Cucumber (Gy14) v2cgybcpeB406
Cp4.1LG03g17300Carg14849Silver-seed gourdcarcpeB0884
Cp4.1LG03g17300Carg06369Silver-seed gourdcarcpeB1331
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG03g17300Cp4.1LG08g02020Cucurbita pepo (Zucchini)cpecpeB482
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG03g17300Cucurbita pepo (Zucchini)cpecpeB184
Cp4.1LG03g17300Cucurbita pepo (Zucchini)cpecpeB243
Cp4.1LG03g17300Cucurbita pepo (Zucchini)cpecpeB339
Cp4.1LG03g17300Cucumber (Gy14) v1cgycpeB0965
Cp4.1LG03g17300Cucurbita maxima (Rimu)cmacpeB402
Cp4.1LG03g17300Cucurbita maxima (Rimu)cmacpeB926
Cp4.1LG03g17300Cucurbita moschata (Rifu)cmocpeB364
Cp4.1LG03g17300Cucurbita moschata (Rifu)cmocpeB860
Cp4.1LG03g17300Wild cucumber (PI 183967)cpecpiB633
Cp4.1LG03g17300Cucumber (Chinese Long) v2cpecuB629
Cp4.1LG03g17300Bottle gourd (USVL1VR-Ls)cpelsiB482
Cp4.1LG03g17300Watermelon (Charleston Gray)cpewcgB565
Cp4.1LG03g17300Watermelon (97103) v1cpewmB625
Cp4.1LG03g17300Silver-seed gourdcarcpeB0327
Cp4.1LG03g17300Melon (DHL92) v3.5.1cpemeB552
Cp4.1LG03g17300Cucumber (Gy14) v2cgybcpeB834
Cp4.1LG03g17300Melon (DHL92) v3.6.1cpemedB655
Cp4.1LG03g17300Silver-seed gourdcarcpeB1505
Cp4.1LG03g17300Cucumber (Chinese Long) v3cpecucB0785
Cp4.1LG03g17300Wax gourdcpewgoB0759