Carg06369 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg06369
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Pectinesterase
Location	Cucurbita_argyrosperma_scaffold_016 : 1232818 .. 1233765 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCCATTAAAAGCGTTTCGGTTCTCGTTCTTGTTCTGGTTCTTGGGTCGGCCATTTTTGCTTACTCGGATGACGAAGTGAAAAATTGGTGCAGCCAAACGCCATATCCAGCTCCATGTGAACATTTTCTCACGGCAAAAAAAACGGCCACAGAAAAAAACCGGCCAATCGCTTCAAAATCCCACTTCCTTAAAGTTCTGGTCGAATCGGCCATCGAGCGAGCCGTTTTGGCCCAAAAAAACGCCGTCGCTCTCGGCCCAAAGTGCCAAAACGACCGAGAAAAATCTGCATGGACCGACTGTATCGAGCTCTACGACCAAATCATTCACCGGCTCAACCGGACCGCCGGTCGGTGCTCTCCGGTCGATGTTCAGACCTGGCTCAGTGCTGCTCTCACAGCTCTCGACACGTGTCGAGCTGGGTTTGAGGAGCTTGGGGTTTCGGCTTTCGGCTACTCTTTAACGGCTAATAATGTTTCCAAGCTGATTAGTGATGGGTTGTCGGTGAATAAGCCGGCTTCGGCTGAGGGGTTTGAGCCGCCGAGTATGAATGAAGGGTTTCCGACGTGGGTTAAGCCGGGAGAAAGGAAGCTGTTGCAGTCGAGCGCTCCGAAAGCTAACGTGGTGGTGGCGAAGGATGGGTCTGGGAGATTTAGGACGGTGAGTGAGGCGGTCGCCGCCGCGAAGGGCGGAGGAAGGTTTGTGATTTATGTGAAGGCCGGGGTTTATAGTGAGAATGTTGAGATTAAGGCTAAGAATGTTATGTTGATCGGCGATGGCATCGGAAAAACTATCATCACCGGAAGTAGGAGCGTCGGCGGCGGTTCCACCACTTTTAAATCCGCCACCGTTGGTAATCCCCCCTTTCTTTTTTATTTTTTTTATAAATAAAATCATAATTAAAATGGGTTTAATTGGGATTTTAGTGAGGTTTAAGAATTCTAA

mRNA sequence

ATGGCAGCCATTAAAAGCGTTTCGGTTCTCGTTCTTGTTCTGGTTCTTGGGTCGGCCATTTTTGCTTACTCGGATGACGAAGTGAAAAATTGGTGCAGCCAAACGCCATATCCAGCTCCATGTGAACATTTTCTCACGGCAAAAAAAACGGCCACAGAAAAAAACCGGCCAATCGCTTCAAAATCCCACTTCCTTAAAGTTCTGGTCGAATCGGCCATCGAGCGAGCCGTTTTGGCCCAAAAAAACGCCGTCGCTCTCGGCCCAAAGTGCCAAAACGACCGAGAAAAATCTGCATGGACCGACTGTATCGAGCTCTACGACCAAATCATTCACCGGCTCAACCGGACCGCCGGTCGGTGCTCTCCGGTCGATGTTCAGACCTGGCTCAGTGCTGCTCTCACAGCTCTCGACACGTGTCGAGCTGGGTTTGAGGAGCTTGGGGTTTCGGCTTTCGGCTACTCTTTAACGGCTAATAATGTTTCCAAGCTGATTAGTGATGGGTTGTCGGTGAATAAGCCGGCTTCGGCTGAGGGGTTTGAGCCGCCGAGTATGAATGAAGGGTTTCCGACGTGGGTTAAGCCGGGAGAAAGGAAGCTGTTGCAGTCGAGCGCTCCGAAAGCTAACGTGGTGGTGGCGAAGGATGGGTCTGGGAGATTTAGGACGGTGAGTGAGGCGGTCGCCGCCGCGAAGGGCGGAGGAAGGTTTGTGATTTATGTGAAGGCCGGGGTTTATAGTGAGAATGTTGAGATTAAGGCTAAGAATGTTATGTTGATCGGCGATGGCATCGGAAAAACTATCATCACCGGAAGTAGGAGCGTCGGCGGCGGTTCCACCACTTTTAAATCCGCCACCGTTGAATTCTAA

Coding sequence (CDS)

ATGGCAGCCATTAAAAGCGTTTCGGTTCTCGTTCTTGTTCTGGTTCTTGGGTCGGCCATTTTTGCTTACTCGGATGACGAAGTGAAAAATTGGTGCAGCCAAACGCCATATCCAGCTCCATGTGAACATTTTCTCACGGCAAAAAAAACGGCCACAGAAAAAAACCGGCCAATCGCTTCAAAATCCCACTTCCTTAAAGTTCTGGTCGAATCGGCCATCGAGCGAGCCGTTTTGGCCCAAAAAAACGCCGTCGCTCTCGGCCCAAAGTGCCAAAACGACCGAGAAAAATCTGCATGGACCGACTGTATCGAGCTCTACGACCAAATCATTCACCGGCTCAACCGGACCGCCGGTCGGTGCTCTCCGGTCGATGTTCAGACCTGGCTCAGTGCTGCTCTCACAGCTCTCGACACGTGTCGAGCTGGGTTTGAGGAGCTTGGGGTTTCGGCTTTCGGCTACTCTTTAACGGCTAATAATGTTTCCAAGCTGATTAGTGATGGGTTGTCGGTGAATAAGCCGGCTTCGGCTGAGGGGTTTGAGCCGCCGAGTATGAATGAAGGGTTTCCGACGTGGGTTAAGCCGGGAGAAAGGAAGCTGTTGCAGTCGAGCGCTCCGAAAGCTAACGTGGTGGTGGCGAAGGATGGGTCTGGGAGATTTAGGACGGTGAGTGAGGCGGTCGCCGCCGCGAAGGGCGGAGGAAGGTTTGTGATTTATGTGAAGGCCGGGGTTTATAGTGAGAATGTTGAGATTAAGGCTAAGAATGTTATGTTGATCGGCGATGGCATCGGAAAAACTATCATCACCGGAAGTAGGAGCGTCGGCGGCGGTTCCACCACTTTTAAATCCGCCACCGTTGAATTCTAA

Protein sequence

MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATVEF

Homology

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BLAST of Carg06369 vs. NCBI nr
Match: XP_023539131.1 (pectinesterase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 547.4 bits (1409), Expect = 3.0e-152
Identity = 281/285 (98.60%), Postives = 283/285 (99.30%), Query Frame = 0


Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60
           MAAIKSVSVLVLVLVLGSAIFAYSD+EVKNWCSQTPYPAPCEHFLTAKKT TEKNRPIAS
Sbjct: 1   MAAIKSVSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIAS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120
           KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC
Sbjct: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120

Query: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180
           SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE
Sbjct: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180

Query: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240
           PPSMNEGFPTWVKPGERKLLQSSAP ANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK
Sbjct: 181 PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240

Query: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTF+SATV
Sbjct: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 285

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BLAST of Carg06369 vs. NCBI nr
Match: XP_022941906.1 (pectinesterase 2 [Cucurbita moschata])

HSP 1 Score: 541.2 bits (1393), Expect = 2.1e-150
Identity = 280/285 (98.25%), Postives = 281/285 (98.60%), Query Frame = 0


Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60
           MAAIK  SVLVLVLVLGS IFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS
Sbjct: 1   MAAIK--SVLVLVLVLGSVIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120
           KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC
Sbjct: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120

Query: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180
           SPVDVQTWLSAALTALDTC+AGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE
Sbjct: 121 SPVDVQTWLSAALTALDTCQAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180

Query: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240
           PPSMNEGFPTWVKPGERKLLQSSAP ANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK
Sbjct: 181 PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240

Query: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV
Sbjct: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 283

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BLAST of Carg06369 vs. NCBI nr
Match: XP_022974184.1 (pectinesterase 2 [Cucurbita maxima])

HSP 1 Score: 531.2 bits (1367), Expect = 2.2e-147
Identity = 271/285 (95.09%), Postives = 277/285 (97.19%), Query Frame = 0


Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60
           M AIKSV VLVLVLVL SAIFAYSD+EVKNWCSQTPYPAPCEHFLTAKKT T+KNRPIAS
Sbjct: 1   MVAIKSVIVLVLVLVLWSAIFAYSDEEVKNWCSQTPYPAPCEHFLTAKKTTTKKNRPIAS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120
           KSHFL+VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDC+ELYDQIIHRLNRTA RC
Sbjct: 61  KSHFLRVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCVELYDQIIHRLNRTADRC 120

Query: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180
           SP+DVQTWLSAALTALDTCR GFEELGVSAFGY LTANNVSKLISDGLSVNKPAS+EGFE
Sbjct: 121 SPIDVQTWLSAALTALDTCRTGFEELGVSAFGYPLTANNVSKLISDGLSVNKPASSEGFE 180

Query: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240
           PPSMNEGFPTWVKPGERKLLQSSAP ANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK
Sbjct: 181 PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240

Query: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV
Sbjct: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 285

[Close]

BLAST of Carg06369 vs. NCBI nr
Match: XP_022975621.1 (probable pectinesterase/pectinesterase inhibitor 17 [Cucurbita maxima])

HSP 1 Score: 531.2 bits (1367), Expect = 2.2e-147
Identity = 271/285 (95.09%), Postives = 277/285 (97.19%), Query Frame = 0


Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60
           M AIKSV VLVLVLVL SAIFAYSD+EVKNWCSQTPYPAPCEHFLTAKKT T+KNRPIAS
Sbjct: 1   MVAIKSVIVLVLVLVLWSAIFAYSDEEVKNWCSQTPYPAPCEHFLTAKKTTTKKNRPIAS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120
           KSHFL+VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDC+ELYDQIIHRLNRTA RC
Sbjct: 61  KSHFLRVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCVELYDQIIHRLNRTADRC 120

Query: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180
           SP+DVQTWLSAALTALDTCR GFEELGVSAFGY LTANNVSKLISDGLSVNKPAS+EGFE
Sbjct: 121 SPIDVQTWLSAALTALDTCRTGFEELGVSAFGYPLTANNVSKLISDGLSVNKPASSEGFE 180

Query: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240
           PPSMNEGFPTWVKPGERKLLQSSAP ANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK
Sbjct: 181 PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240

Query: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV
Sbjct: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 285

[Close]

BLAST of Carg06369 vs. NCBI nr
Match: XP_004133929.1 (PREDICTED: pectinesterase 2 [Cucumis sativus] >KGN56617.1 hypothetical protein Csa_3G126810 [Cucumis sativus])

HSP 1 Score: 426.4 bits (1095), Expect = 7.7e-116
Identity = 205/277 (74.01%), Postives = 244/277 (88.09%), Query Frame = 0


Query: 9   VLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVL 68
           V+++VL+  S I  YS+ E+KNWCSQTPYPAPCE FL  K  AT K  PI +KSHF ++L
Sbjct: 10  VVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTK--ATTKKTPITTKSHFFEIL 69

Query: 69  VESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRCSPVDVQTW 128
           VE+A+ERAV A KNA++LGPKC+N +EK+AWTDC++LYDQII RLNRT+ RCSP D QTW
Sbjct: 70  VETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPADAQTW 129

Query: 129 LSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGF 188
           LSAALTAL+TCR GFEELG+SAFGY LTANNVSKLISDGLSVNKPAS EG+EP +M +GF
Sbjct: 130 LSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTMTDGF 189

Query: 189 PTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGVYSENV 248
           PTWV PG RKLLQS +PKA+VVVA+DGSG F+TV +A++AAKGGGRFVIY+K+GVY+EN+
Sbjct: 190 PTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVYNENL 249

Query: 249 EIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           +IKAKNVM++GDGIGKTIITGSRSVGGGSTTF+SATV
Sbjct: 250 DIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATV 284

[Close]

BLAST of Carg06369 vs. TAIR10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 314.7 bits (805), Expect = 5.9e-86
Identity = 161/282 (57.09%), Postives = 207/282 (73.40%), Query Frame = 0


Query: 7   VSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLK 66
           ++ ++L +++ S +  Y+  +VK WCSQTP P PCE+FL    T    N PI S+S FLK
Sbjct: 9   INFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFL----THNSNNEPIKSESEFLK 68

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           + ++  ++RA+LA+ +A  LGPKC++ REK+AW DCI+LYD  + ++N T     +CS +
Sbjct: 69  ISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKL 128

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT LDTCRAGF ELGV+     L +NNVS L+ + L++NK      + PP 
Sbjct: 129 DAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKVPF--NYTPPE 188

Query: 187 MNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGV 246
             +GFP+WVKPG+RKLLQSS PK N VVAKDGSG F+T+ EA+ AA G GRFVIYVK GV
Sbjct: 189 -KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGV 248

Query: 247 YSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           YSEN+EI+ KNVML GDGIGKTIITGS+SVGGG+TTF SATV
Sbjct: 249 YSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 283

[Close]

BLAST of Carg06369 vs. TAIR10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 190.3 bits (482), Expect = 1.7e-48
Identity = 121/299 (40.47%), Postives = 168/299 (56.19%), Query Frame = 0


Query: 6   SVSVLVLVLVLGSAIFAYSDDEVKN---WCSQTPYPAPCEHFLTAKKTATEKNRPIASKS 65
           S+ +L+++L+    +   +D    N   WC +TPYP PC+ +           R     S
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGF----RLPTQIS 72

Query: 66  HFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNR------- 125
            F  +LVE+A++RAV A          C + ++++   DCI LY   + +LNR       
Sbjct: 73  EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSS 132

Query: 126 -TAGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTAN-NVSKLISDGLSVNKP 185
            T  RC+  D QTWLS ALT  +TCR G  +L VS F   + +N  +S LIS+ L+VN  
Sbjct: 133 KTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGA 192

Query: 186 ASAEGFEPPSM--NEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAA-- 245
               G    +   ++GFPTWV   ER+LLQ  + +AN+VVAKDGSG F+TV  A+  A  
Sbjct: 193 LLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGR 252

Query: 246 --KGGGRFVIYVKAGVYSENVEIKAK--NVMLIGDGIGKTIITGSRSVGGGSTTFKSAT 285
                GRFVIYVK G+Y EN+ ++    N+ML+GDG+  TIITG RSV GG TT+ SAT
Sbjct: 253 RKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSAT 307

[Close]

BLAST of Carg06369 vs. TAIR10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 188.3 bits (477), Expect = 6.4e-48
Identity = 119/301 (39.53%), Postives = 173/301 (57.48%), Query Frame = 0


Query: 3   AIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKS 62
           +I  + +++LVL+    +   +D    +WC +TPYP PC+ +    K      +P    S
Sbjct: 9   SILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYF---KNHNGFQQP-TQLS 68

Query: 63  HFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRT------ 122
            F  +LVE+A++RA+ A+      G  C + ++++   DCI+LY   I +LNRT      
Sbjct: 69  EFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSP 128

Query: 123 ----AGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTAN-NVSKLISDGLSVN 182
               A  C+  D QTWLS ALT  +TCR G  +L V+ F   + +N  +S LIS+ L+VN
Sbjct: 129 KAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVN 188

Query: 183 KPASAEGFE--PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAA 242
                 G +    +  +GFPTW+   +++LL+  A +AN+VVAKDGSG F TV  A+  A
Sbjct: 189 GALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVA 248

Query: 243 ----KGGGRFVIYVKAGVYSENVEIKAKN--VMLIGDGIGKTIITGSRSVGGGSTTFKSA 285
                  GRFVIYVK G+Y EN+ ++  N  +ML+GDG+  TIITG RSV GG TT+ SA
Sbjct: 249 GRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSA 303

[Close]

BLAST of Carg06369 vs. TAIR10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 185.3 bits (469), Expect = 5.4e-47
Identity = 110/278 (39.57%), Postives = 161/278 (57.91%), Query Frame = 0


Query: 31  WCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVLVESAIERAVLAQKNAVALGPKC 90
           WC++TP+   C ++           RP   +S FL++LV+ A+++AV+     V  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 91  QNDREKSAWTDCIELYDQIIHRLNRTAG----------RCSPVDVQTWLSAALTALDTCR 150
            N++ K+AW+DC+ L+   + +LNRT            +C+  D QTWLS A T ++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 151 AGFEELGVSAFGYSLTAN-NVSKLISDGLSVNKPASAEGFEPPSMN--EGFPTWVKPGER 210
           +G E+L VS F   + +N N+S LI + L+VN     +     + N  E FP+WV   ER
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 211 KLLQSSA---PKANVVVAKDGSGRFRTVSEAV---AAAKGGGRFVIYVKAGVYSENVEI- 270
           +LL S++      ++VVA+D SG FR++  A+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 271 -KAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATVEF 288
               N+ML+GDG  KTIIT  RSV  G TT+ SAT  F
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGF 364

[Close]

BLAST of Carg06369 vs. TAIR10
Match: AT4G00190.1 (pectin methylesterase 38)

HSP 1 Score: 167.5 bits (423), Expect = 1.2e-41
Identity = 113/263 (42.97%), Postives = 153/263 (58.17%), Query Frame = 0


Query: 32  CSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVLVESAIERAVLAQKNAVALGPKCQ 91
           C +TP+P  C       KT   K++PI S + FL+V VE  ++ AV A+ +   L P+  
Sbjct: 8   CDETPHPGEC-------KTLLIKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFG 67

Query: 92  NDREKSAWTDCIELYDQIIHRLNRTA----GRCSPVDVQTWLSAALTALDTCRAGFEELG 151
           +   K AW +C++LY+Q IHRLN +       CS  DVQ WLS ALT LDTC+    ELG
Sbjct: 68  S---KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELG 127

Query: 152 VSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGFPTWVKPGERKLLQSSAPKA 211
           VS+  +SL +  +   + + L++NK     G E           +     K L S   K 
Sbjct: 128 VSS--HSLESITID--VINTLAINKRMEQNGKE---------FGISKITMKTL-SIGEKV 187

Query: 212 NVVVAKDGSGRFRTVSEAVAAA----KGGGRFVIYVKAGVYSE--NVEIKAKNVMLIGDG 271
           +VVVA+DGSG ++T+ EAV  A    KG  R+VI+VK GVY E  NV IK+ N+M+ GDG
Sbjct: 188 DVVVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMITGDG 246

Query: 272 IGKTIITGSRSVGGGSTTFKSAT 285
           IGKTIITG +S G G +T+KSAT
Sbjct: 248 IGKTIITGDKSKGRGFSTYKSAT 246

[Close]

BLAST of Carg06369 vs. Swiss-Prot
Match: sp|O22149|PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 314.7 bits (805), Expect = 1.1e-84
Identity = 161/282 (57.09%), Postives = 207/282 (73.40%), Query Frame = 0


Query: 7   VSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLK 66
           ++ ++L +++ S +  Y+  +VK WCSQTP P PCE+FL    T    N PI S+S FLK
Sbjct: 9   INFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFL----THNSNNEPIKSESEFLK 68

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           + ++  ++RA+LA+ +A  LGPKC++ REK+AW DCI+LYD  + ++N T     +CS +
Sbjct: 69  ISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKL 128

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT LDTCRAGF ELGV+     L +NNVS L+ + L++NK      + PP 
Sbjct: 129 DAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKVPF--NYTPPE 188

Query: 187 MNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGV 246
             +GFP+WVKPG+RKLLQSS PK N VVAKDGSG F+T+ EA+ AA G GRFVIYVK GV
Sbjct: 189 -KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGV 248

Query: 247 YSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           YSEN+EI+ KNVML GDGIGKTIITGS+SVGGG+TTF SATV
Sbjct: 249 YSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 283

[Close]

BLAST of Carg06369 vs. Swiss-Prot
Match: sp|O04887|PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 4.6e-80
Identity = 155/284 (54.58%), Postives = 204/284 (71.83%), Query Frame = 0


Query: 7   VSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLK 66
           ++V +++  L    F YS +EVK+WC +TP P PCE+FLT K   T     I   + F K
Sbjct: 7   ITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTS----IKQDTDFYK 66

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGR---CSPV 126
           + ++ A+ERA  AQ     LG KC+N+REK+AW DC ELY+  + +LN+T+     C+ V
Sbjct: 67  ISLQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKV 126

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT L+TCRA  E+LGV  +   L +NNV+KLIS+ LS+NK      +  PS
Sbjct: 127 DKQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKVP----YNEPS 186

Query: 187 MNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAK--GGGRFVIYVKA 246
             +GFPTWVKPG+RKLLQ++ P+AN+VVA+DGSG  +T+ EAVAAA   GG R+VIY+KA
Sbjct: 187 YKDGFPTWVKPGDRKLLQTT-PRANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKA 246

Query: 247 GVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           G Y+EN+E+K KN+M +GDGIGKTIITGS+SVGGG+TTFKSATV
Sbjct: 247 GTYNENIEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATV 281

[Close]

BLAST of Carg06369 vs. Swiss-Prot
Match: sp|Q9FHN4|PME60_ARATH (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.0e-47
Identity = 121/299 (40.47%), Postives = 168/299 (56.19%), Query Frame = 0


Query: 6   SVSVLVLVLVLGSAIFAYSDDEVKN---WCSQTPYPAPCEHFLTAKKTATEKNRPIASKS 65
           S+ +L+++L+    +   +D    N   WC +TPYP PC+ +           R     S
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGF----RLPTQIS 72

Query: 66  HFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNR------- 125
            F  +LVE+A++RAV A          C + ++++   DCI LY   + +LNR       
Sbjct: 73  EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSS 132

Query: 126 -TAGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTAN-NVSKLISDGLSVNKP 185
            T  RC+  D QTWLS ALT  +TCR G  +L VS F   + +N  +S LIS+ L+VN  
Sbjct: 133 KTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGA 192

Query: 186 ASAEGFEPPSM--NEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAA-- 245
               G    +   ++GFPTWV   ER+LLQ  + +AN+VVAKDGSG F+TV  A+  A  
Sbjct: 193 LLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGR 252

Query: 246 --KGGGRFVIYVKAGVYSENVEIKAK--NVMLIGDGIGKTIITGSRSVGGGSTTFKSAT 285
                GRFVIYVK G+Y EN+ ++    N+ML+GDG+  TIITG RSV GG TT+ SAT
Sbjct: 253 RKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSAT 307

[Close]

BLAST of Carg06369 vs. Swiss-Prot
Match: sp|Q9FHN5|PME59_ARATH (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.2e-46
Identity = 119/301 (39.53%), Postives = 173/301 (57.48%), Query Frame = 0


Query: 3   AIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKS 62
           +I  + +++LVL+    +   +D    +WC +TPYP PC+ +    K      +P    S
Sbjct: 9   SILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYF---KNHNGFQQP-TQLS 68

Query: 63  HFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRT------ 122
            F  +LVE+A++RA+ A+      G  C + ++++   DCI+LY   I +LNRT      
Sbjct: 69  EFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSP 128

Query: 123 ----AGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTAN-NVSKLISDGLSVN 182
               A  C+  D QTWLS ALT  +TCR G  +L V+ F   + +N  +S LIS+ L+VN
Sbjct: 129 KAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVN 188

Query: 183 KPASAEGFE--PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAA 242
                 G +    +  +GFPTW+   +++LL+  A +AN+VVAKDGSG F TV  A+  A
Sbjct: 189 GALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVA 248

Query: 243 ----KGGGRFVIYVKAGVYSENVEIKAKN--VMLIGDGIGKTIITGSRSVGGGSTTFKSA 285
                  GRFVIYVK G+Y EN+ ++  N  +ML+GDG+  TIITG RSV GG TT+ SA
Sbjct: 249 GRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSA 303

[Close]

BLAST of Carg06369 vs. Swiss-Prot
Match: sp|Q9STY3|PME33_ARATH (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 9.7e-46
Identity = 110/278 (39.57%), Postives = 161/278 (57.91%), Query Frame = 0


Query: 31  WCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVLVESAIERAVLAQKNAVALGPKC 90
           WC++TP+   C ++           RP   +S FL++LV+ A+++AV+     V  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 91  QNDREKSAWTDCIELYDQIIHRLNRTAG----------RCSPVDVQTWLSAALTALDTCR 150
            N++ K+AW+DC+ L+   + +LNRT            +C+  D QTWLS A T ++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 151 AGFEELGVSAFGYSLTAN-NVSKLISDGLSVNKPASAEGFEPPSMN--EGFPTWVKPGER 210
           +G E+L VS F   + +N N+S LI + L+VN     +     + N  E FP+WV   ER
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 211 KLLQSSA---PKANVVVAKDGSGRFRTVSEAV---AAAKGGGRFVIYVKAGVYSENVEI- 270
           +LL S++      ++VVA+D SG FR++  A+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 271 -KAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATVEF 288
               N+ML+GDG  KTIIT  RSV  G TT+ SAT  F
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGF 364

[Close]

BLAST of Carg06369 vs. TrEMBL
Match: tr|A0A0A0L7N4|A0A0A0L7N4_CUCSA (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126810 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 5.1e-116
Identity = 205/277 (74.01%), Postives = 244/277 (88.09%), Query Frame = 0


Query: 9   VLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVL 68
           V+++VL+  S I  YS+ E+KNWCSQTPYPAPCE FL  K  AT K  PI +KSHF ++L
Sbjct: 10  VVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTK--ATTKKTPITTKSHFFEIL 69

Query: 69  VESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRCSPVDVQTW 128
           VE+A+ERAV A KNA++LGPKC+N +EK+AWTDC++LYDQII RLNRT+ RCSP D QTW
Sbjct: 70  VETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPADAQTW 129

Query: 129 LSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGF 188
           LSAALTAL+TCR GFEELG+SAFGY LTANNVSKLISDGLSVNKPAS EG+EP +M +GF
Sbjct: 130 LSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTMTDGF 189

Query: 189 PTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGVYSENV 248
           PTWV PG RKLLQS +PKA+VVVA+DGSG F+TV +A++AAKGGGRFVIY+K+GVY+EN+
Sbjct: 190 PTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVYNENL 249

Query: 249 EIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           +IKAKNVM++GDGIGKTIITGSRSVGGGSTTF+SATV
Sbjct: 250 DIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATV 284

[Close]

BLAST of Carg06369 vs. TrEMBL
Match: tr|A0A1S3AVW4|A0A1S3AVW4_CUCME (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483370 PE=3 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.3e-114
Identity = 204/278 (73.38%), Postives = 248/278 (89.21%), Query Frame = 0


Query: 9   VLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVL 68
           V+++VL+    +  YS+ E+KNWCSQTPYPAPCE FL   K +T+K+ PI +KSHF ++L
Sbjct: 10  VVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKT-KASTKKSTPITTKSHFFEIL 69

Query: 69  VESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRCSPVDVQTW 128
           VE+A+ERAV A KNA++LGPKC+N +EK+AWTDC++LYD+II RLNRT+ RC+P D QTW
Sbjct: 70  VETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTPADAQTW 129

Query: 129 LSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS-MNEG 188
           LSAALTAL+TCR GF+ELG+SAFGY LTANNVSKLISDGLSVNKPAS EG+EP + M++G
Sbjct: 130 LSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTIMDDG 189

Query: 189 FPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGVYSEN 248
           FPTWV+PG+RKLLQS +PKANVVVA+DGSG F+TV EAVAAAKGGGRFVIYVK+GVY+EN
Sbjct: 190 FPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYVKSGVYNEN 249

Query: 249 VEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           ++IKAKNVM++GDGIGKTIITGSRSVGGGSTTF+SATV
Sbjct: 250 LDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATV 286

[Close]

BLAST of Carg06369 vs. TrEMBL
Match: tr|A0A0L9VS61|A0A0L9VS61_PHAAN (Pectinesterase OS=Phaseolus angularis OX=3914 GN=LR48_Vigan11g061200 PE=3 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.3e-84
Identity = 166/274 (60.58%), Postives = 210/274 (76.64%), Query Frame = 0


Query: 18  SAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVLVESAIERAV 77
           S +F+YS  ++++WC+QTPYP PCE++LT        N+PI SKS FLKV ++ A+ERA 
Sbjct: 28  SVVFSYSLKDIQSWCNQTPYPQPCEYYLTNHAF----NKPIKSKSDFLKVSLQLALERAE 87

Query: 78  LAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPVDVQTWLSAALT 137
             Q N  ++GPKC+ND EKSAW DCI+LY   I RLN+T     +C+  D QTWLS ALT
Sbjct: 88  RGQLNTHSIGPKCRNDHEKSAWNDCIQLYQYTIQRLNKTLDPYTKCTQTDTQTWLSTALT 147

Query: 138 ALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGFPTWVKP 197
            L+TCR GF ELGV+ +   L +NNV+KL+S+ LS+NK  +++ F+PPS  EGFPTWVKP
Sbjct: 148 NLETCRIGFYELGVTDYILPLLSNNVTKLLSNTLSLNKDPNSQ-FKPPSTQEGFPTWVKP 207

Query: 198 GERKLLQSS--APKANVVVAKDGSGRFRTVSEAV-AAAKGGGRFVIYVKAGVYSENVEIK 257
           G+RKLLQSS  A KANV+VAKDGSG++ TV  AV AA    GR+VIYVK+GVY+E VE+K
Sbjct: 208 GDRKLLQSSSAASKANVIVAKDGSGKYTTVKAAVDAAPSSSGRYVIYVKSGVYNEQVEVK 267

Query: 258 AKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           +KN+ML+GDGIGKTIITGS+SVGGGSTTF SATV
Sbjct: 268 SKNIMLVGDGIGKTIITGSKSVGGGSTTFNSATV 296

[Close]

BLAST of Carg06369 vs. TrEMBL
Match: tr|A0A0S3SY54|A0A0S3SY54_PHAAN (Pectinesterase OS=Vigna angularis var. angularis OX=157739 GN=Vigan.09G122000 PE=3 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.3e-84
Identity = 166/274 (60.58%), Postives = 210/274 (76.64%), Query Frame = 0


Query: 18  SAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVLVESAIERAV 77
           S +F+YS  ++++WC+QTPYP PCE++LT        N+PI SKS FLKV ++ A+ERA 
Sbjct: 28  SVVFSYSLKDIQSWCNQTPYPQPCEYYLTNHAF----NKPIKSKSDFLKVSLQLALERAE 87

Query: 78  LAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPVDVQTWLSAALT 137
             Q N  ++GPKC+ND EKSAW DCI+LY   I RLN+T     +C+  D QTWLS ALT
Sbjct: 88  RGQLNTHSIGPKCRNDHEKSAWNDCIQLYQYTIQRLNKTLDPYTKCTQTDTQTWLSTALT 147

Query: 138 ALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGFPTWVKP 197
            L+TCR GF ELGV+ +   L +NNV+KL+S+ LS+NK  +++ F+PPS  EGFPTWVKP
Sbjct: 148 NLETCRIGFYELGVTDYILPLLSNNVTKLLSNTLSLNKDPNSQ-FKPPSTQEGFPTWVKP 207

Query: 198 GERKLLQSS--APKANVVVAKDGSGRFRTVSEAV-AAAKGGGRFVIYVKAGVYSENVEIK 257
           G+RKLLQSS  A KANV+VAKDGSG++ TV  AV AA    GR+VIYVK+GVY+E VE+K
Sbjct: 208 GDRKLLQSSSAASKANVIVAKDGSGKYTTVKAAVDAAPSSSGRYVIYVKSGVYNEQVEVK 267

Query: 258 AKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           +KN+ML+GDGIGKTIITGS+SVGGGSTTF SATV
Sbjct: 268 SKNIMLVGDGIGKTIITGSKSVGGGSTTFNSATV 296

[Close]

BLAST of Carg06369 vs. TrEMBL
Match: tr|V7ANF1|V7ANF1_PHAVU (Pectinesterase OS=Phaseolus vulgaris OX=3885 GN=PHAVU_010G079900g PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 6.7e-84
Identity = 168/286 (58.74%), Postives = 217/286 (75.87%), Query Frame = 0


Query: 7   VSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLK 66
           +++L++  +  S  F+YS  ++++WC+QTPYP PCE++LT+       N+PI SKS FL+
Sbjct: 13  ITLLLVPFLFSSIAFSYSFKDIQSWCNQTPYPQPCEYYLTSHTF----NKPIKSKSDFLQ 72

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           V ++ A+ERA   Q N  ++GPKC+N  EKSAWTDCIELYD  I RLN+T     +C+  
Sbjct: 73  VSLQLALERAERGQLNTHSIGPKCRNVHEKSAWTDCIELYDYTIQRLNKTLDPYTKCTQT 132

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT L+TCRAG  ELGV  +   L +NNV+KL+S+ LS+NK ++   F+PPS
Sbjct: 133 DTQTWLSTALTNLETCRAGLYELGVQDYMLPLMSNNVTKLLSNTLSLNKDSNYP-FKPPS 192

Query: 187 MNEGFPTWVKPGERKLLQSS--APKANVVVAKDGSGRFRTVSEAVAAA--KGGGRFVIYV 246
             EG+PTWVKPG+RKLLQSS  A +ANVVVAKDGSG++ TV  AV AA     GR+VIYV
Sbjct: 193 SQEGYPTWVKPGDRKLLQSSSAASQANVVVAKDGSGKYTTVKAAVDAAPSSSSGRYVIYV 252

Query: 247 KAGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           K+GVY+E VEIK+KN+ML+GDGIGKTIITGS+SVGGGSTTF+SATV
Sbjct: 253 KSGVYNEQVEIKSKNIMLVGDGIGKTIITGSKSVGGGSTTFRSATV 293

The following BLAST results are available for this feature:

BLAST of Carg06369 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023539131.1	3.0e-152	98.60	pectinesterase 2 [Cucurbita pepo subsp. pepo]	[more]
XP_022941906.1	2.1e-150	98.25	pectinesterase 2 [Cucurbita moschata]	[more]
XP_022974184.1	2.2e-147	95.09	pectinesterase 2 [Cucurbita maxima]	[more]
XP_022975621.1	2.2e-147	95.09	probable pectinesterase/pectinesterase inhibitor 17 [Cucurbita maxima]	[more]
XP_004133929.1	7.7e-116	74.01	PREDICTED: pectinesterase 2 [Cucumis sativus] >KGN56617.1 hypothetical protein C...	[more]

BLAST of Carg06369 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G45220.1	5.9e-86	57.09	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51500.1	1.7e-48	40.47	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51490.1	6.4e-48	39.53	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G47400.1	5.4e-47	39.57	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G00190.1	1.2e-41	42.97	pectin methylesterase 38	[more]

BLAST of Carg06369 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O22149\|PME17_ARATH	1.1e-84	57.09	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
sp\|O04887\|PME2_CITSI	4.6e-80	54.58	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
sp\|Q9FHN4\|PME60_ARATH	3.0e-47	40.47	Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3...	[more]
sp\|Q9FHN5\|PME59_ARATH	1.2e-46	39.53	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
sp\|Q9STY3\|PME33_ARATH	9.7e-46	39.57	Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Carg06369 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L7N4\|A0A0A0L7N4_CUCSA	5.1e-116	74.01	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126810 PE=3 SV=1	[more]
tr\|A0A1S3AVW4\|A0A1S3AVW4_CUCME	1.3e-114	73.38	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483370 PE=3 SV=1	[more]
tr\|A0A0L9VS61\|A0A0L9VS61_PHAAN	1.3e-84	60.58	Pectinesterase OS=Phaseolus angularis OX=3914 GN=LR48_Vigan11g061200 PE=3 SV=1	[more]
tr\|A0A0S3SY54\|A0A0S3SY54_PHAAN	1.3e-84	60.58	Pectinesterase OS=Vigna angularis var. angularis OX=157739 GN=Vigan.09G122000 PE...	[more]
tr\|V7ANF1\|V7ANF1_PHAVU	6.7e-84	58.74	Pectinesterase OS=Phaseolus vulgaris OX=3885 GN=PHAVU_010G079900g PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0042545	cell wall modification

Vocabulary: **Molecular Function**
Term	Definition
GO:0030599	pectinesterase activity
GO:0004857	enzyme inhibitor activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005618	cell wall

Vocabulary: **INTERPRO**
Term	Definition
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold
IPR000070	Pectinesterase_cat
IPR035513	Invertase/methylesterase_inhib
IPR006501	Pectinesterase_inhib_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005618	cell wall
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06369-RA	Carg06369-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 23..170 e-value: 3.8E-31 score: 119.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 7..162 e-value: 3.0E-24 score: 83.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 27..166 e-value: 2.1E-23 score: 83.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	G3DSA:1.20.140.40		coord: 22..172 e-value: 2.6E-14 score: 55.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 26..171
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 208..285 e-value: 2.5E-24 score: 85.4
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 201..287 e-value: 2.3E-24 score: 88.2
None	No IPR available	PANTHER	PTHR31707	FAMILY NOT NAMED	coord: 18..285
None	No IPR available	PANTHER	PTHR31707:SF11	PECTINESTERASE/PECTINESTERASE INHIBITOR 17-RELATED	coord: 18..285
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 31..176 e-value: 2.22555E-28 score: 106.368
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	SSF51126	Pectin lyase-like	coord: 202..285

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg06369	Cla021325	Watermelon (97103) v1	carwmB0961
Carg06369	Csa3G126810	Cucumber (Chinese Long) v2	carcuB0975
Carg06369	MELO3C006208	Melon (DHL92) v3.5.1	carmeB0941
Carg06369	ClCG05G002440	Watermelon (Charleston Gray)	carwcgB0935
Carg06369	CSPI03G09730	Wild cucumber (PI 183967)	carcpiB0998
Carg06369	Cucsa.257480	Cucumber (Gy14) v1	carcgyB1077
Carg06369	CmaCh06G011470	Cucurbita maxima (Rimu)	carcmaB1325
Carg06369	CmaCh14G019670	Cucurbita maxima (Rimu)	carcmaB1320
Carg06369	CmoCh06G011760	Cucurbita moschata (Rifu)	carcmoB1288
Carg06369	CmoCh14G020280	Cucurbita moschata (Rifu)	carcmoB1284
Carg06369	Lsi05G019420	Bottle gourd (USVL1VR-Ls)	carlsiB878
Carg06369	Cp4.1LG03g17300	Cucurbita pepo (Zucchini)	carcpeB1331
Carg06369	Cp4.1LG08g02020	Cucurbita pepo (Zucchini)	carcpeB1333
Carg06369	CsGy3G009820	Cucumber (Gy14) v2	carcgybB0948
Carg06369	MELO3C006208.2	Melon (DHL92) v3.6.1	carmedB0947
Carg06369	CsaV3_3G009870	Cucumber (Chinese Long) v3	carcucB1003
Carg06369	Cla97C05G082940	Watermelon (97103) v2	carwmbB1008
Carg06369	BhiUN737G9	Wax gourd	carwgoB1115

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg06369	Carg14849	Silver-seed gourd	carcarB549

The following block(s) are covering this gene:


Gene	Organism	Block
Carg06369	Silver-seed gourd	carcarB266
Carg06369	Cucumber (Gy14) v2	carcgybB0949
Carg06369	Cucumber (Gy14) v1	carcgyB1079
Carg06369	Cucurbita maxima (Rimu)	carcmaB1321
Carg06369	Cucurbita moschata (Rifu)	carcmoB1285
Carg06369	Cucurbita pepo (Zucchini)	carcpeB1328
Carg06369	Wild cucumber (PI 183967)	carcpiB0999
Carg06369	Cucumber (Chinese Long) v2	carcuB0976
Carg06369	Cucumber (Chinese Long) v3	carcucB1004
Carg06369	Bottle gourd (USVL1VR-Ls)	carlsiB875
Carg06369	Melon (DHL92) v3.5.1	carmeB0938
Carg06369	Melon (DHL92) v3.6.1	carmedB0944
Carg06369	Watermelon (Charleston Gray)	carwcgB0937
Carg06369	Watermelon (97103) v1	carwmB0963
Carg06369	Watermelon (97103) v2	carwmbB1010
Carg06369	Wax gourd	carwgoB1113