Cp4.1LG08g02020 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG08g02020
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectinesterase
LocationCp4.1LG08 : 3029761 .. 3031128 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAACAGGCAGAACCATGACAAGATAACTAAAAACAAGGCAACTAAAAACTTAAGGGTCAGTTTCTCTCTCATAAGACTAATTTCTCTCTCCTCTGACCTTGTTCCCTATAAAAAGAAACCTCCCATCCCATCCCACCATTAACCCGAACTAAAAAAAATGGCAGCCATTAAAAGCGTTTCCGTTCTCGTTCTTGTTCTTGTTCTTGGGTCGGCCATTTTTGCTTACTCGGATAACGAAGTGAAAAATTGGTGCAGCCAAACGCCATATCCAGCTCCATGTGAACATTTTCTCACGGCAAAAAAAACCGGCACAGAAAAAAACCGGCCAATCGCTTCAAAATCCCACTTCCTTAAAGTTCTGGTCGAATCGGCCATCGAGCGAGCCGTTTTGGCCCAAAAAAACGCCGTCGCTCTCGGGCCAAAGTGCCAAAACGACCGAGAAAAATCTGCATGGACCGACTGTATCGAGCTCTACGACCAAATCATTCACCGGCTCAACCGGACCGCCGGCCGCTGCTCCCCGGTCGATGTTCAAACCTGGCTCAGTGCTGCTCTCACAGCTCTTGACACGTGTCGAGCTGGGTTTGAGGAGCTTGGGGTTTCGGCTTTCGGCTACTCTTTAACGGCTAATAATGTTTCGAAGCTGATTAGTGATGGGTTGTCGGTGAATAAGCCGGCTTCGGCTGAGGGGTTTGAGCCGCCGAGTATGAATGAAGGGTTTCCGACGTGGGTTAAGCCGGGAGAAAGGAAGCTGTTGCAGTCGAGCGCTCCGAATGCTAACGTGGTGGTGGCGAAGGATGGGTCTGGGAGATTTAGGACGGTGAGTGAGGCGGTCGCCGCCGCGAAGGGCGGAGGGAGGTTTGTGATTTATGTGAAGGCCGGGGTTTATAGTGAGAATGTTGAGATTAAGGCTAAGAATGTTATGTTGATCGGCGATGGCATCGGAAAAACTATCATCACCGGAAGTAGAAGCGTCGGCGGCGGTTCCACCACTTTTAGATCCGCCACCGTTGGTAATTCTCCCCTTCCTTTTTTATTTTTTATTTATAAATAAAATCATAATGAAAATGGGTTTAAATGGGATTTTAGTGAGGTTTAAGAATTCTAATTTTAAAAAATGGGTTTAGAGTTTTTTTTTTTTCCCAATAATAAATAATAATTCTTAAAAATAAAAATAATTAATAATAATTTTTATTCAAATGGAAGCTTTGGAAAACACCACCCATTTACAAAAGCTTAAAATTTTAATTTTTTATATAATAATAAATAATAGATTTTATAAGATTTCTTAACAATTTAAATTATTATTTAAAAAAAAATATTAAATATACCTAATTCTTTTGATAATGTTTGACTTAGTTCATTAG

mRNA sequence

CAAACAGGCAGAACCATGACAAGATAACTAAAAACAAGGCAACTAAAAACTTAAGGGTCAGTTTCTCTCTCATAAGACTAATTTCTCTCTCCTCTGACCTTGTTCCCTATAAAAAGAAACCTCCCATCCCATCCCACCATTAACCCGAACTAAAAAAAATGGCAGCCATTAAAAGCGTTTCCGTTCTCGTTCTTGTTCTTGTTCTTGGGTCGGCCATTTTTGCTTACTCGGATAACGAAGTGAAAAATTGGTGCAGCCAAACGCCATATCCAGCTCCATGTGAACATTTTCTCACGGCAAAAAAAACCGGCACAGAAAAAAACCGGCCAATCGCTTCAAAATCCCACTTCCTTAAAGTTCTGGTCGAATCGGCCATCGAGCGAGCCGTTTTGGCCCAAAAAAACGCCGTCGCTCTCGGGCCAAAGTGCCAAAACGACCGAGAAAAATCTGCATGGACCGACTGTATCGAGCTCTACGACCAAATCATTCACCGGCTCAACCGGACCGCCGGCCGCTGCTCCCCGGTCGATGTTCAAACCTGGCTCAGTGCTGCTCTCACAGCTCTTGACACGTGTCGAGCTGGGTTTGAGGAGCTTGGGGTTTCGGCTTTCGGCTACTCTTTAACGGCTAATAATGTTTCGAAGCTGATTAGTGATGGGTTGTCGGTGAATAAGCCGGCTTCGGCTGAGGGGTTTGAGCCGCCGAGTATGAATGAAGGGTTTCCGACGTGGGTTAAGCCGGGAGAAAGGAAGCTGTTGCAGTCGAGCGCTCCGAATGCTAACGTGGTGGTGGCGAAGGATGGGTCTGGGAGATTTAGGACGGTGAGTGAGGCGGTCGCCGCCGCGAAGGGCGGAGGGAGGTTTGTGATTTATGTGAAGGCCGGGGTTTATAGTGAGAATGTTGAGATTAAGGCTAAGAATGTTATGTTGATCGGCGATGGCATCGGAAAAACTATCATCACCGGAAGTAGAAGCGTCGGCGGCGGTTCCACCACTTTTAGATCCGCCACCGTTGTTCATTAG

Coding sequence (CDS)

ATGGCAGCCATTAAAAGCGTTTCCGTTCTCGTTCTTGTTCTTGTTCTTGGGTCGGCCATTTTTGCTTACTCGGATAACGAAGTGAAAAATTGGTGCAGCCAAACGCCATATCCAGCTCCATGTGAACATTTTCTCACGGCAAAAAAAACCGGCACAGAAAAAAACCGGCCAATCGCTTCAAAATCCCACTTCCTTAAAGTTCTGGTCGAATCGGCCATCGAGCGAGCCGTTTTGGCCCAAAAAAACGCCGTCGCTCTCGGGCCAAAGTGCCAAAACGACCGAGAAAAATCTGCATGGACCGACTGTATCGAGCTCTACGACCAAATCATTCACCGGCTCAACCGGACCGCCGGCCGCTGCTCCCCGGTCGATGTTCAAACCTGGCTCAGTGCTGCTCTCACAGCTCTTGACACGTGTCGAGCTGGGTTTGAGGAGCTTGGGGTTTCGGCTTTCGGCTACTCTTTAACGGCTAATAATGTTTCGAAGCTGATTAGTGATGGGTTGTCGGTGAATAAGCCGGCTTCGGCTGAGGGGTTTGAGCCGCCGAGTATGAATGAAGGGTTTCCGACGTGGGTTAAGCCGGGAGAAAGGAAGCTGTTGCAGTCGAGCGCTCCGAATGCTAACGTGGTGGTGGCGAAGGATGGGTCTGGGAGATTTAGGACGGTGAGTGAGGCGGTCGCCGCCGCGAAGGGCGGAGGGAGGTTTGTGATTTATGTGAAGGCCGGGGTTTATAGTGAGAATGTTGAGATTAAGGCTAAGAATGTTATGTTGATCGGCGATGGCATCGGAAAAACTATCATCACCGGAAGTAGAAGCGTCGGCGGCGGTTCCACCACTTTTAGATCCGCCACCGTTGTTCATTAG

Protein sequence

MAAIKSVSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATVVH
Homology
[Close]
BLAST of Cp4.1LG08g02020 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 313.9 bits (803), Expect = 1.8e-84
Identity = 160/282 (56.74%), Postives = 206/282 (73.05%), Query Frame = 1 

Query: 7   VSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLK 66
           ++ ++L +++ S +  Y+  +VK WCSQTP P PCE+FLT        N PI S+S FLK
Sbjct: 9   INFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSN----NEPIKSESEFLK 68

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           + ++  ++RA+LA+ +A  LGPKC++ REK+AW DCI+LYD  + ++N T     +CS +
Sbjct: 69  ISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKL 128

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT LDTCRAGF ELGV+     L +NNVS L+ + L++NK      + PP 
Sbjct: 129 DAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKVPF--NYTPPE 188

Query: 187 MNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGV 246
             +GFP+WVKPG+RKLLQSS P  N VVAKDGSG F+T+ EA+ AA G GRFVIYVK GV
Sbjct: 189 -KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGV 248

Query: 247 YSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           YSEN+EI+ KNVML GDGIGKTIITGS+SVGGG+TTF SATV
Sbjct: 249 YSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 283

[Close]
BLAST of Cp4.1LG08g02020 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 3.9e-79
Identity = 154/284 (54.23%), Postives = 202/284 (71.13%), Query Frame = 1 

Query: 7   VSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLK 66
           ++V +++  L    F YS  EVK+WC +TP P PCE+FLT K   T     I   + F K
Sbjct: 7   ITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTS----IKQDTDFYK 66

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGR---CSPV 126
           + ++ A+ERA  AQ     LG KC+N+REK+AW DC ELY+  + +LN+T+     C+ V
Sbjct: 67  ISLQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKV 126

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT L+TCRA  E+LGV  +   L +NNV+KLIS+ LS+NK      +  PS
Sbjct: 127 DKQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKVP----YNEPS 186

Query: 187 MNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAK--GGGRFVIYVKA 246
             +GFPTWVKPG+RKLLQ++ P AN+VVA+DGSG  +T+ EAVAAA   GG R+VIY+KA
Sbjct: 187 YKDGFPTWVKPGDRKLLQTT-PRANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKA 246

Query: 247 GVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           G Y+EN+E+K KN+M +GDGIGKTIITGS+SVGGG+TTF+SATV
Sbjct: 247 GTYNENIEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATV 281

[Close]
BLAST of Cp4.1LG08g02020 vs. Swiss-Prot
Match: PME60_ARATH (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana GN=PME60 PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 4.6e-48
Identity = 123/299 (41.14%), Postives = 169/299 (56.52%), Query Frame = 1 

Query: 6   SVSVLVLVLVLGSAIFAYSDNEVKN---WCSQTPYPAPCEHFLTAKKTGTEKNRPIASKS 65
           S+ +L+++L+    +   +D    N   WC +TPYP PC+ +   K +G      I   S
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFI-KHSGFRLPTQI---S 72

Query: 66  HFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRT------ 125
            F  +LVE+A++RAV A          C + ++++   DCI LY   + +LNRT      
Sbjct: 73  EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSS 132

Query: 126 --AGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANN-VSKLISDGLSVNKP 185
               RC+  D QTWLS ALT  +TCR G  +L VS F   + +N  +S LIS+ L+VN  
Sbjct: 133 KTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGA 192

Query: 186 ASAEGFEPPSMNE--GFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAA-- 245
               G    +  +  GFPTWV   ER+LLQ  +  AN+VVAKDGSG F+TV  A+  A  
Sbjct: 193 LLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGR 252

Query: 246 --KGGGRFVIYVKAGVYSENVEIKAK--NVMLIGDGIGKTIITGSRSVGGGSTTFRSAT 285
                GRFVIYVK G+Y EN+ ++    N+ML+GDG+  TIITG RSV GG TT+ SAT
Sbjct: 253 RKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSAT 307

[Close]
BLAST of Cp4.1LG08g02020 vs. Swiss-Prot
Match: PME59_ARATH (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=PME59 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 6.6e-47
Identity = 119/301 (39.53%), Postives = 172/301 (57.14%), Query Frame = 1 

Query: 3   AIKSVSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKS 62
           +I  + +++LVL+    +   +D    +WC +TPYP PC+ +    K      +P    S
Sbjct: 9   SILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYF---KNHNGFQQP-TQLS 68

Query: 63  HFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRT------ 122
            F  +LVE+A++RA+ A+      G  C + ++++   DCI+LY   I +LNRT      
Sbjct: 69  EFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSP 128

Query: 123 ----AGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTAN-NVSKLISDGLSVN 182
               A  C+  D QTWLS ALT  +TCR G  +L V+ F   + +N  +S LIS+ L+VN
Sbjct: 129 KAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVN 188

Query: 183 KPASAEGFE--PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAA 242
                 G +    +  +GFPTW+   +++LL+  A  AN+VVAKDGSG F TV  A+  A
Sbjct: 189 GALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVA 248

Query: 243 ----KGGGRFVIYVKAGVYSENVEIKAKN--VMLIGDGIGKTIITGSRSVGGGSTTFRSA 285
                  GRFVIYVK G+Y EN+ ++  N  +ML+GDG+  TIITG RSV GG TT+ SA
Sbjct: 249 GRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSA 303

[Close]
BLAST of Cp4.1LG08g02020 vs. Swiss-Prot
Match: PME33_ARATH (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana GN=PME33 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.3e-46
Identity = 109/275 (39.64%), Postives = 161/275 (58.55%), Query Frame = 1 

Query: 31  WCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLKVLVESAIERAVLAQKNAVALGPKC 90
           WC++TP+   C ++           RP   +S FL++LV+ A+++AV+     V  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 91  QNDREKSAWTDCIELYDQIIHRLNRTAG----------RCSPVDVQTWLSAALTALDTCR 150
            N++ K+AW+DC+ L+   + +LNRT            +C+  D QTWLS A T ++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 151 AGFEELGVSAFGYSLTAN-NVSKLISDGLSVNKPASAEGFEPPSMN--EGFPTWVKPGER 210
           +G E+L VS F   + +N N+S LI + L+VN     +     + N  E FP+WV   ER
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 211 KLLQSSA---PNANVVVAKDGSGRFRTVSEAV---AAAKGGGRFVIYVKAGVYSENVEI- 270
           +LL S++    + ++VVA+D SG FR++  A+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 271 -KAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSAT 285
               N+ML+GDG  KTIIT  RSV  G TT+ SAT
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSAT 361

[Close]
BLAST of Cp4.1LG08g02020 vs. TrEMBL
Match: A0A0A0L7N4_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G126810 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 4.7e-116
Identity = 204/277 (73.65%), Postives = 242/277 (87.36%), Query Frame = 1 

Query: 9   VLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLKVL 68
           V+++VL+  S I  YS+ E+KNWCSQTPYPAPCE FL  K   T K  PI +KSHF ++L
Sbjct: 10  VVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTK--ATTKKTPITTKSHFFEIL 69

Query: 69  VESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRCSPVDVQTW 128
           VE+A+ERAV A KNA++LGPKC+N +EK+AWTDC++LYDQII RLNRT+ RCSP D QTW
Sbjct: 70  VETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPADAQTW 129

Query: 129 LSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGF 188
           LSAALTAL+TCR GFEELG+SAFGY LTANNVSKLISDGLSVNKPAS EG+EP +M +GF
Sbjct: 130 LSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTMTDGF 189

Query: 189 PTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGVYSENV 248
           PTWV PG RKLLQS +P A+VVVA+DGSG F+TV +A++AAKGGGRFVIY+K+GVY+EN+
Sbjct: 190 PTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVYNENL 249

Query: 249 EIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           +IKAKNVM++GDGIGKTIITGSRSVGGGSTTFRSATV
Sbjct: 250 DIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATV 284

[Close]
BLAST of Cp4.1LG08g02020 vs. TrEMBL
Match: A0A0L9VS61_PHAAN (Pectinesterase OS=Phaseolus angularis GN=LR48_Vigan11g061200 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 5.1e-86
Identity = 168/285 (58.95%), Postives = 217/285 (76.14%), Query Frame = 1 

Query: 7   VSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLK 66
           +S+L++ L+  S +F+YS  ++++WC+QTPYP PCE++LT        N+PI SKS FLK
Sbjct: 17  ISLLLIPLLFSSVVFSYSLKDIQSWCNQTPYPQPCEYYLTNHAF----NKPIKSKSDFLK 76

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           V ++ A+ERA   Q N  ++GPKC+ND EKSAW DCI+LY   I RLN+T     +C+  
Sbjct: 77  VSLQLALERAERGQLNTHSIGPKCRNDHEKSAWNDCIQLYQYTIQRLNKTLDPYTKCTQT 136

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT L+TCR GF ELGV+ +   L +NNV+KL+S+ LS+NK  +++ F+PPS
Sbjct: 137 DTQTWLSTALTNLETCRIGFYELGVTDYILPLLSNNVTKLLSNTLSLNKDPNSQ-FKPPS 196

Query: 187 MNEGFPTWVKPGERKLLQSS--APNANVVVAKDGSGRFRTVSEAV-AAAKGGGRFVIYVK 246
             EGFPTWVKPG+RKLLQSS  A  ANV+VAKDGSG++ TV  AV AA    GR+VIYVK
Sbjct: 197 TQEGFPTWVKPGDRKLLQSSSAASKANVIVAKDGSGKYTTVKAAVDAAPSSSGRYVIYVK 256

Query: 247 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           +GVY+E VE+K+KN+ML+GDGIGKTIITGS+SVGGGSTTF SATV
Sbjct: 257 SGVYNEQVEVKSKNIMLVGDGIGKTIITGSKSVGGGSTTFNSATV 296

[Close]
BLAST of Cp4.1LG08g02020 vs. TrEMBL
Match: A0A0S3SY54_PHAAN (Pectinesterase OS=Vigna angularis var. angularis GN=Vigan.09G122000 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 5.1e-86
Identity = 168/285 (58.95%), Postives = 217/285 (76.14%), Query Frame = 1 

Query: 7   VSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLK 66
           +S+L++ L+  S +F+YS  ++++WC+QTPYP PCE++LT        N+PI SKS FLK
Sbjct: 17  ISLLLIPLLFSSVVFSYSLKDIQSWCNQTPYPQPCEYYLTNHAF----NKPIKSKSDFLK 76

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           V ++ A+ERA   Q N  ++GPKC+ND EKSAW DCI+LY   I RLN+T     +C+  
Sbjct: 77  VSLQLALERAERGQLNTHSIGPKCRNDHEKSAWNDCIQLYQYTIQRLNKTLDPYTKCTQT 136

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT L+TCR GF ELGV+ +   L +NNV+KL+S+ LS+NK  +++ F+PPS
Sbjct: 137 DTQTWLSTALTNLETCRIGFYELGVTDYILPLLSNNVTKLLSNTLSLNKDPNSQ-FKPPS 196

Query: 187 MNEGFPTWVKPGERKLLQSS--APNANVVVAKDGSGRFRTVSEAV-AAAKGGGRFVIYVK 246
             EGFPTWVKPG+RKLLQSS  A  ANV+VAKDGSG++ TV  AV AA    GR+VIYVK
Sbjct: 197 TQEGFPTWVKPGDRKLLQSSSAASKANVIVAKDGSGKYTTVKAAVDAAPSSSGRYVIYVK 256

Query: 247 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           +GVY+E VE+K+KN+ML+GDGIGKTIITGS+SVGGGSTTF SATV
Sbjct: 257 SGVYNEQVEVKSKNIMLVGDGIGKTIITGSKSVGGGSTTFNSATV 296

[Close]
BLAST of Cp4.1LG08g02020 vs. TrEMBL
Match: V7ANF1_PHAVU (Pectinesterase OS=Phaseolus vulgaris GN=PHAVU_010G079900g PE=4 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 9.5e-85
Identity = 169/286 (59.09%), Postives = 216/286 (75.52%), Query Frame = 1 

Query: 7   VSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLK 66
           +++L++  +  S  F+YS  ++++WC+QTPYP PCE++LT+       N+PI SKS FL+
Sbjct: 13  ITLLLVPFLFSSIAFSYSFKDIQSWCNQTPYPQPCEYYLTSHTF----NKPIKSKSDFLQ 72

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           V ++ A+ERA   Q N  ++GPKC+N  EKSAWTDCIELYD  I RLN+T     +C+  
Sbjct: 73  VSLQLALERAERGQLNTHSIGPKCRNVHEKSAWTDCIELYDYTIQRLNKTLDPYTKCTQT 132

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT L+TCRAG  ELGV  +   L +NNV+KL+S+ LS+NK ++   F+PPS
Sbjct: 133 DTQTWLSTALTNLETCRAGLYELGVQDYMLPLMSNNVTKLLSNTLSLNKDSNYP-FKPPS 192

Query: 187 MNEGFPTWVKPGERKLLQSS--APNANVVVAKDGSGRFRTVSEAVAAA--KGGGRFVIYV 246
             EG+PTWVKPG+RKLLQSS  A  ANVVVAKDGSG++ TV  AV AA     GR+VIYV
Sbjct: 193 SQEGYPTWVKPGDRKLLQSSSAASQANVVVAKDGSGKYTTVKAAVDAAPSSSSGRYVIYV 252

Query: 247 KAGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           K+GVY+E VEIK+KN+ML+GDGIGKTIITGS+SVGGGSTTFRSATV
Sbjct: 253 KSGVYNEQVEIKSKNIMLVGDGIGKTIITGSKSVGGGSTTFRSATV 293

[Close]
BLAST of Cp4.1LG08g02020 vs. TrEMBL
Match: D7LCG8_ARALL (Pectinesterase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_670486 PE=4 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 9.9e-82
Identity = 160/282 (56.74%), Postives = 206/282 (73.05%), Query Frame = 1 

Query: 7   VSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLK 66
           ++ + L +++ S +  Y+  +VK WCSQTP P PCE+FLT        N+PI S+S FL+
Sbjct: 9   ITFVFLCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSN----NKPIKSESEFLE 68

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           + ++ A++RAVLA+ +A  LGPKC++ REK+AW DCI+LYD  + ++N T     +CS  
Sbjct: 69  ISMKLALDRAVLAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKT 128

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT LDTCRAGF ELGV+     L +NNVS L+ + L++NK      + PP 
Sbjct: 129 DAQTWLSTALTNLDTCRAGFLELGVTDVVLPLMSNNVSNLLCNTLAINKVPF--NYTPPE 188

Query: 187 MNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGV 246
             +GFP+WVKPG+RKLLQSS P  N VVAKDGSG F+T+ +A+ AA G GRFVIYVK GV
Sbjct: 189 -KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKDAINAASGSGRFVIYVKQGV 248

Query: 247 YSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           YSEN+EI+ KNVML GDGIGKTIITGS+SVGGG+TTF SATV
Sbjct: 249 YSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 283

[Close]
BLAST of Cp4.1LG08g02020 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 313.9 bits (803), Expect = 1.0e-85
Identity = 160/282 (56.74%), Postives = 206/282 (73.05%), Query Frame = 1 

Query: 7   VSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLK 66
           ++ ++L +++ S +  Y+  +VK WCSQTP P PCE+FLT        N PI S+S FLK
Sbjct: 9   INFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSN----NEPIKSESEFLK 68

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           + ++  ++RA+LA+ +A  LGPKC++ REK+AW DCI+LYD  + ++N T     +CS +
Sbjct: 69  ISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKL 128

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT LDTCRAGF ELGV+     L +NNVS L+ + L++NK      + PP 
Sbjct: 129 DAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKVPF--NYTPPE 188

Query: 187 MNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGV 246
             +GFP+WVKPG+RKLLQSS P  N VVAKDGSG F+T+ EA+ AA G GRFVIYVK GV
Sbjct: 189 -KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGV 248

Query: 247 YSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           YSEN+EI+ KNVML GDGIGKTIITGS+SVGGG+TTF SATV
Sbjct: 249 YSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 283

[Close]
BLAST of Cp4.1LG08g02020 vs. TAIR10
Match: AT5G51500.1 (AT5G51500.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 193.0 bits (489), Expect = 2.6e-49
Identity = 123/299 (41.14%), Postives = 169/299 (56.52%), Query Frame = 1 

Query: 6   SVSVLVLVLVLGSAIFAYSDNEVKN---WCSQTPYPAPCEHFLTAKKTGTEKNRPIASKS 65
           S+ +L+++L+    +   +D    N   WC +TPYP PC+ +   K +G      I   S
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFI-KHSGFRLPTQI---S 72

Query: 66  HFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRT------ 125
            F  +LVE+A++RAV A          C + ++++   DCI LY   + +LNRT      
Sbjct: 73  EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSS 132

Query: 126 --AGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANN-VSKLISDGLSVNKP 185
               RC+  D QTWLS ALT  +TCR G  +L VS F   + +N  +S LIS+ L+VN  
Sbjct: 133 KTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGA 192

Query: 186 ASAEGFEPPSMNE--GFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAA-- 245
               G    +  +  GFPTWV   ER+LLQ  +  AN+VVAKDGSG F+TV  A+  A  
Sbjct: 193 LLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGR 252

Query: 246 --KGGGRFVIYVKAGVYSENVEIKAK--NVMLIGDGIGKTIITGSRSVGGGSTTFRSAT 285
                GRFVIYVK G+Y EN+ ++    N+ML+GDG+  TIITG RSV GG TT+ SAT
Sbjct: 253 RKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSAT 307

[Close]
BLAST of Cp4.1LG08g02020 vs. TAIR10
Match: AT5G51490.1 (AT5G51490.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 189.1 bits (479), Expect = 3.7e-48
Identity = 119/301 (39.53%), Postives = 172/301 (57.14%), Query Frame = 1 

Query: 3   AIKSVSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKS 62
           +I  + +++LVL+    +   +D    +WC +TPYP PC+ +    K      +P    S
Sbjct: 9   SILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYF---KNHNGFQQP-TQLS 68

Query: 63  HFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRT------ 122
            F  +LVE+A++RA+ A+      G  C + ++++   DCI+LY   I +LNRT      
Sbjct: 69  EFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSP 128

Query: 123 ----AGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTAN-NVSKLISDGLSVN 182
               A  C+  D QTWLS ALT  +TCR G  +L V+ F   + +N  +S LIS+ L+VN
Sbjct: 129 KAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVN 188

Query: 183 KPASAEGFE--PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAA 242
                 G +    +  +GFPTW+   +++LL+  A  AN+VVAKDGSG F TV  A+  A
Sbjct: 189 GALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVA 248

Query: 243 ----KGGGRFVIYVKAGVYSENVEIKAKN--VMLIGDGIGKTIITGSRSVGGGSTTFRSA 285
                  GRFVIYVK G+Y EN+ ++  N  +ML+GDG+  TIITG RSV GG TT+ SA
Sbjct: 249 GRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSA 303

[Close]
BLAST of Cp4.1LG08g02020 vs. TAIR10
Match: AT3G47400.1 (AT3G47400.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 186.8 bits (473), Expect = 1.9e-47
Identity = 109/275 (39.64%), Postives = 161/275 (58.55%), Query Frame = 1 

Query: 31  WCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLKVLVESAIERAVLAQKNAVALGPKC 90
           WC++TP+   C ++           RP   +S FL++LV+ A+++AV+     V  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 91  QNDREKSAWTDCIELYDQIIHRLNRTAG----------RCSPVDVQTWLSAALTALDTCR 150
            N++ K+AW+DC+ L+   + +LNRT            +C+  D QTWLS A T ++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 151 AGFEELGVSAFGYSLTAN-NVSKLISDGLSVNKPASAEGFEPPSMN--EGFPTWVKPGER 210
           +G E+L VS F   + +N N+S LI + L+VN     +     + N  E FP+WV   ER
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 211 KLLQSSA---PNANVVVAKDGSGRFRTVSEAV---AAAKGGGRFVIYVKAGVYSENVEI- 270
           +LL S++    + ++VVA+D SG FR++  A+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 271 -KAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSAT 285
               N+ML+GDG  KTIIT  RSV  G TT+ SAT
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSAT 361

[Close]
BLAST of Cp4.1LG08g02020 vs. TAIR10
Match: AT4G00190.1 (AT4G00190.1 pectin methylesterase 38)

HSP 1 Score: 165.6 bits (418), Expect = 4.4e-41
Identity = 111/265 (41.89%), Postives = 153/265 (57.74%), Query Frame = 1 

Query: 32  CSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLKVLVESAIERAVLAQKNAVALGPKCQ 91
           C +TP+P  C+  L        K++PI S + FL+V VE  ++ AV A+ +   L P+  
Sbjct: 8   CDETPHPGECKTLLI-------KHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFG 67

Query: 92  NDREKSAWTDCIELYDQIIHRLNRTA----GRCSPVDVQTWLSAALTALDTCRAGFEELG 151
           +   K AW +C++LY+Q IHRLN +       CS  DVQ WLS ALT LDTC+    ELG
Sbjct: 68  S---KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELG 127

Query: 152 VSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGFPTWVKPGERKLLQSSAPNA 211
           VS+  +SL +  +   + + L++NK     G E           +     K L S     
Sbjct: 128 VSS--HSLESITID--VINTLAINKRMEQNGKE---------FGISKITMKTL-SIGEKV 187

Query: 212 NVVVAKDGSGRFRTVSEAVAAA----KGGGRFVIYVKAGVYSE--NVEIKAKNVMLIGDG 271
           +VVVA+DGSG ++T+ EAV  A    KG  R+VI+VK GVY E  NV IK+ N+M+ GDG
Sbjct: 188 DVVVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMITGDG 247

Query: 272 IGKTIITGSRSVGGGSTTFRSATVV 287
           IGKTIITG +S G G +T++SAT V
Sbjct: 248 IGKTIITGDKSKGRGFSTYKSATFV 248

[Close]
BLAST of Cp4.1LG08g02020 vs. NCBI nr
Match: gi|449432283|ref|XP_004133929.1| (PREDICTED: pectinesterase 2 [Cucumis sativus])

HSP 1 Score: 425.6 bits (1093), Expect = 6.7e-116
Identity = 204/277 (73.65%), Postives = 242/277 (87.36%), Query Frame = 1 

Query: 9   VLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLKVL 68
           V+++VL+  S I  YS+ E+KNWCSQTPYPAPCE FL  K   T K  PI +KSHF ++L
Sbjct: 10  VVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTK--ATTKKTPITTKSHFFEIL 69

Query: 69  VESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRCSPVDVQTW 128
           VE+A+ERAV A KNA++LGPKC+N +EK+AWTDC++LYDQII RLNRT+ RCSP D QTW
Sbjct: 70  VETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPADAQTW 129

Query: 129 LSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGF 188
           LSAALTAL+TCR GFEELG+SAFGY LTANNVSKLISDGLSVNKPAS EG+EP +M +GF
Sbjct: 130 LSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTMTDGF 189

Query: 189 PTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGVYSENV 248
           PTWV PG RKLLQS +P A+VVVA+DGSG F+TV +A++AAKGGGRFVIY+K+GVY+EN+
Sbjct: 190 PTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVYNENL 249

Query: 249 EIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           +IKAKNVM++GDGIGKTIITGSRSVGGGSTTFRSATV
Sbjct: 250 DIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATV 284

[Close]
BLAST of Cp4.1LG08g02020 vs. NCBI nr
Match: gi|659075527|ref|XP_008438193.1| (PREDICTED: pectinesterase 2 [Cucumis melo])

HSP 1 Score: 421.8 bits (1083), Expect = 9.7e-115
Identity = 204/278 (73.38%), Postives = 247/278 (88.85%), Query Frame = 1 

Query: 9   VLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLKVL 68
           V+++VL+    +  YS+ E+KNWCSQTPYPAPCE FL  K + T+K+ PI +KSHF ++L
Sbjct: 10  VVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKAS-TKKSTPITTKSHFFEIL 69

Query: 69  VESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRCSPVDVQTW 128
           VE+A+ERAV A KNA++LGPKC+N +EK+AWTDC++LYD+II RLNRT+ RC+P D QTW
Sbjct: 70  VETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTPADAQTW 129

Query: 129 LSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS-MNEG 188
           LSAALTAL+TCR GF+ELG+SAFGY LTANNVSKLISDGLSVNKPAS EG+EP + M++G
Sbjct: 130 LSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTIMDDG 189

Query: 189 FPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGVYSEN 248
           FPTWV+PG+RKLLQS +P ANVVVA+DGSG F+TV EAVAAAKGGGRFVIYVK+GVY+EN
Sbjct: 190 FPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYVKSGVYNEN 249

Query: 249 VEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           ++IKAKNVM++GDGIGKTIITGSRSVGGGSTTFRSATV
Sbjct: 250 LDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATV 286

[Close]
BLAST of Cp4.1LG08g02020 vs. NCBI nr
Match: gi|920718382|gb|KOM57579.1| (hypothetical protein LR48_Vigan11g061200 [Vigna angularis])

HSP 1 Score: 325.9 bits (834), Expect = 7.3e-86
Identity = 168/285 (58.95%), Postives = 217/285 (76.14%), Query Frame = 1 

Query: 7   VSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLK 66
           +S+L++ L+  S +F+YS  ++++WC+QTPYP PCE++LT        N+PI SKS FLK
Sbjct: 17  ISLLLIPLLFSSVVFSYSLKDIQSWCNQTPYPQPCEYYLTNHAF----NKPIKSKSDFLK 76

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           V ++ A+ERA   Q N  ++GPKC+ND EKSAW DCI+LY   I RLN+T     +C+  
Sbjct: 77  VSLQLALERAERGQLNTHSIGPKCRNDHEKSAWNDCIQLYQYTIQRLNKTLDPYTKCTQT 136

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT L+TCR GF ELGV+ +   L +NNV+KL+S+ LS+NK  +++ F+PPS
Sbjct: 137 DTQTWLSTALTNLETCRIGFYELGVTDYILPLLSNNVTKLLSNTLSLNKDPNSQ-FKPPS 196

Query: 187 MNEGFPTWVKPGERKLLQSS--APNANVVVAKDGSGRFRTVSEAV-AAAKGGGRFVIYVK 246
             EGFPTWVKPG+RKLLQSS  A  ANV+VAKDGSG++ TV  AV AA    GR+VIYVK
Sbjct: 197 TQEGFPTWVKPGDRKLLQSSSAASKANVIVAKDGSGKYTTVKAAVDAAPSSSGRYVIYVK 256

Query: 247 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           +GVY+E VE+K+KN+ML+GDGIGKTIITGS+SVGGGSTTF SATV
Sbjct: 257 SGVYNEQVEVKSKNIMLVGDGIGKTIITGSKSVGGGSTTFNSATV 296

[Close]
BLAST of Cp4.1LG08g02020 vs. NCBI nr
Match: gi|593265306|ref|XP_007134831.1| (hypothetical protein PHAVU_010G079900g [Phaseolus vulgaris])

HSP 1 Score: 321.6 bits (823), Expect = 1.4e-84
Identity = 169/286 (59.09%), Postives = 216/286 (75.52%), Query Frame = 1 

Query: 7   VSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIASKSHFLK 66
           +++L++  +  S  F+YS  ++++WC+QTPYP PCE++LT+       N+PI SKS FL+
Sbjct: 13  ITLLLVPFLFSSIAFSYSFKDIQSWCNQTPYPQPCEYYLTSHTF----NKPIKSKSDFLQ 72

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           V ++ A+ERA   Q N  ++GPKC+N  EKSAWTDCIELYD  I RLN+T     +C+  
Sbjct: 73  VSLQLALERAERGQLNTHSIGPKCRNVHEKSAWTDCIELYDYTIQRLNKTLDPYTKCTQT 132

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT L+TCRAG  ELGV  +   L +NNV+KL+S+ LS+NK ++   F+PPS
Sbjct: 133 DTQTWLSTALTNLETCRAGLYELGVQDYMLPLMSNNVTKLLSNTLSLNKDSNYP-FKPPS 192

Query: 187 MNEGFPTWVKPGERKLLQSS--APNANVVVAKDGSGRFRTVSEAVAAA--KGGGRFVIYV 246
             EG+PTWVKPG+RKLLQSS  A  ANVVVAKDGSG++ TV  AV AA     GR+VIYV
Sbjct: 193 SQEGYPTWVKPGDRKLLQSSSAASQANVVVAKDGSGKYTTVKAAVDAAPSSSSGRYVIYV 252

Query: 247 KAGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           K+GVY+E VEIK+KN+ML+GDGIGKTIITGS+SVGGGSTTFRSATV
Sbjct: 253 KSGVYNEQVEIKSKNIMLVGDGIGKTIITGSKSVGGGSTTFRSATV 293

[Close]
BLAST of Cp4.1LG08g02020 vs. NCBI nr
Match: gi|729354701|ref|XP_010544633.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 17 [Tarenaya hassleriana])

HSP 1 Score: 321.6 bits (823), Expect = 1.4e-84
Identity = 170/291 (58.42%), Postives = 210/291 (72.16%), Query Frame = 1 

Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIAS 60
           MA    V++    + LG  +F Y   +VK+WCS+TP P PCE+FLT        +RPI S
Sbjct: 1   MALRAYVTIFFFCIFLGPTVFGYDQKDVKSWCSKTPNPKPCEYFLTHNPN----HRPIKS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG-- 120
           +S FLK+ ++ A++RA+LA+ +A  LGPKC++ REK+AW DC++LYD  + +LN T    
Sbjct: 61  ESEFLKISMKLALDRALLAKSHAFTLGPKCRDSREKAAWEDCLKLYDYTVSKLNETVDPY 120

Query: 121 -RCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAE 180
            +CS VD QTWLS+ALT L+TCR GF ELGV  F   L +NNVSKLIS+ L++NK     
Sbjct: 121 VKCSKVDAQTWLSSALTNLETCRTGFIELGVPDFLLPLMSNNVSKLISNTLAINKVPFNY 180

Query: 181 GFEPPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAK---GGGR 240
               PS   GFP WV+PG+RKLLQSS P ANVVVAKDGSG FRT+ EAV AA    G GR
Sbjct: 181 ---TPSQENGFPEWVRPGDRKLLQSSTPQANVVVAKDGSGNFRTIKEAVNAASRRSGSGR 240

Query: 241 FVIYVKAGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 286
           FVIYVK GVY+EN+EI+AKN+ML GDGIGKTIITGS+SVGGGSTTF SATV
Sbjct: 241 FVIYVKQGVYNENIEIRAKNIMLRGDGIGKTIITGSKSVGGGSTTFNSATV 284

The following BLAST results are available for this feature:
BLAST of Cp4.1LG08g02020 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PME17_ARATH1.8e-8456.74Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=P... [more]
PME2_CITSI3.9e-7954.23Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1[more]
PME60_ARATH4.6e-4841.14Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana GN=P... [more]
PME59_ARATH6.6e-4739.53Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=P... [more]
PME33_ARATH3.3e-4639.64Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana GN=P... [more]
BLAST of Cp4.1LG08g02020 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L7N4_CUCSA4.7e-11673.65Pectinesterase OS=Cucumis sativus GN=Csa_3G126810 PE=4 SV=1[more]
A0A0L9VS61_PHAAN5.1e-8658.95Pectinesterase OS=Phaseolus angularis GN=LR48_Vigan11g061200 PE=4 SV=1[more]
A0A0S3SY54_PHAAN5.1e-8658.95Pectinesterase OS=Vigna angularis var. angularis GN=Vigan.09G122000 PE=4 SV=1[more]
V7ANF1_PHAVU9.5e-8559.09Pectinesterase OS=Phaseolus vulgaris GN=PHAVU_010G079900g PE=4 SV=1[more]
D7LCG8_ARALL9.9e-8256.74Pectinesterase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_670486 PE=4 SV=... [more]
BLAST of Cp4.1LG08g02020 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G45220.11.0e-8556.74 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G51500.12.6e-4941.14 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G51490.13.7e-4839.53 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G47400.11.9e-4739.64 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G00190.14.4e-4141.89 pectin methylesterase 38[more]
BLAST of Cp4.1LG08g02020 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449432283|ref|XP_004133929.1|6.7e-11673.65PREDICTED: pectinesterase 2 [Cucumis sativus][more]
gi|659075527|ref|XP_008438193.1|9.7e-11573.38PREDICTED: pectinesterase 2 [Cucumis melo][more]
gi|920718382|gb|KOM57579.1|7.3e-8658.95hypothetical protein LR48_Vigan11g061200 [Vigna angularis][more]
gi|593265306|ref|XP_007134831.1|1.4e-8459.09hypothetical protein PHAVU_010G079900g [Phaseolus vulgaris][more]
gi|729354701|ref|XP_010544633.1|1.4e-8458.42PREDICTED: probable pectinesterase/pectinesterase inhibitor 17 [Tarenaya hassler... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR006501Pectinesterase_inhib_dom
IPR000070Pectinesterase_cat
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g02020.1Cp4.1LG08g02020.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 208..286
score: 1.9
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 25..171
score: 1.0
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 27..168
score: 6.0
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 23..170
score: 3.3
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 7..162
score: 2.7
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 26..171
score: 8.63
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 202..286
score: 2.89
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 203..286
score: 4.6
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 1..286
score: 1.2E
NoneNo IPR availablePANTHERPTHR31707:SF11PECTINESTERASE/PECTINESTERASE INHIBITOR 17-RELATEDcoord: 1..286
score: 1.2E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG08g02020Cucsa.257480Cucumber (Gy14) v1cgycpeB0687
Cp4.1LG08g02020CmaCh14G019670Cucurbita maxima (Rimu)cmacpeB292
Cp4.1LG08g02020CmaCh06G011470Cucurbita maxima (Rimu)cmacpeB854
Cp4.1LG08g02020CmoCh06G011760Cucurbita moschata (Rifu)cmocpeB796
Cp4.1LG08g02020CmoCh14G020280Cucurbita moschata (Rifu)cmocpeB254
Cp4.1LG08g02020Cla021325Watermelon (97103) v1cpewmB845
Cp4.1LG08g02020Csa3G126810Cucumber (Chinese Long) v2cpecuB859
Cp4.1LG08g02020MELO3C006208Melon (DHL92) v3.5.1cpemeB813
Cp4.1LG08g02020ClCG05G002440Watermelon (Charleston Gray)cpewcgB787
Cp4.1LG08g02020CSPI03G09730Wild cucumber (PI 183967)cpecpiB861
Cp4.1LG08g02020Lsi05G019420Bottle gourd (USVL1VR-Ls)cpelsiB714
Cp4.1LG08g02020MELO3C006208.2Melon (DHL92) v3.6.1cpemedB951
Cp4.1LG08g02020CsaV3_3G009870Cucumber (Chinese Long) v3cpecucB1056
Cp4.1LG08g02020BhiUN737G9Wax gourdcpewgoB1103
Cp4.1LG08g02020CsGy3G009820Cucumber (Gy14) v2cgybcpeB454
Cp4.1LG08g02020Carg06369Silver-seed gourdcarcpeB1333
Cp4.1LG08g02020Carg14849Silver-seed gourdcarcpeB0885
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG08g02020Cp4.1LG03g17300Cucurbita pepo (Zucchini)cpecpeB482
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG08g02020Cucumber (Chinese Long) v3cpecucB1077
Cp4.1LG08g02020Wax gourdcpewgoB1097
Cp4.1LG08g02020Cucurbita pepo (Zucchini)cpecpeB194
Cp4.1LG08g02020Cucurbita pepo (Zucchini)cpecpeB344
Cp4.1LG08g02020Cucumber (Gy14) v1cgycpeB0969
Cp4.1LG08g02020Cucurbita maxima (Rimu)cmacpeB411
Cp4.1LG08g02020Cucurbita moschata (Rifu)cmocpeB373
Cp4.1LG08g02020Wild cucumber (PI 183967)cpecpiB879
Cp4.1LG08g02020Cucumber (Chinese Long) v2cpecuB877
Cp4.1LG08g02020Bottle gourd (USVL1VR-Ls)cpelsiB696
Cp4.1LG08g02020Watermelon (Charleston Gray)cpewcgB790
Cp4.1LG08g02020Watermelon (97103) v1cpewmB854
Cp4.1LG08g02020Melon (DHL92) v3.5.1cpemeB791
Cp4.1LG08g02020Cucumber (Gy14) v2cgybcpeB889
Cp4.1LG08g02020Melon (DHL92) v3.6.1cpemedB932
Cp4.1LG08g02020Silver-seed gourdcarcpeB0329