Cucsa.188140 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.188140
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	E3 ubiquitin-protein ligase RING1
Location	scaffold01294 : 41798 .. 42337 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCAAATCACTCTCAACAATTTCACTATCTCCTAAATTCCTCTCTATCTTCTTTCTTGATACCTTGCGATGTCTTTTTCGCTACTGCCTCCAGTATCCTCATCGCTGTTTTCTCTGGCACCGGTGTCGCACAAGACTTCTTGGACACGGCTCTGCCTGACGTTCTCTCTGTCGCCGCTGATATCGCATGCAATCCTCTTAATGCTGGCCGTACCATACTTCCGTTGGCGGTGTCCGTTTTAGTTGTTTCGCCCTACAATGAACGCCAGGAAATTGGGCGAGTCTTACGGGAATCATCCCCGCAAGCGTTTAAGACGTTGCCAGCTAGTGAGATGGCAATCGAAGGTTTGAAGAACGTGGAGATTGATGTTGGTGGAGAGTTGTTGATTGGAGAATGTAGGATATGTTTGGATGAACTCATGAATGGAATGGAAGTTACGCGATTGCCGTGCGCTCATCTTTATCATAGAGACTGTATTGTTAAATGGCTGGAGACGAGTCATTTGTGCCCCTTGTGCCGGTACGCAATGCCGCTGAGCTGA

mRNA sequence

TCAAATCACTCTCAACAATTTCACTATCTCCTAAATTCCTCTCTATCTTCTTTCTTGATACCTTGCGATGTCTTTTTCGCTACTGCCTCCAGTATCCTCATCGCTGTTTTCTCTGGCACCGGTGTCGCACAAGACTTCTTGGACACGGCTCTGCCTGACGTTCTCTCTGTCGCCGCTGATATCGCATGCAATCCTCTTAATGCTGGCCGTACCATACTTCCGTTGGCGGTGTCCGTTTTAGTTGTTTCGCCCTACAATGAACGCCAGGAAATTGGGCGAGTCTTACGGGAATCATCCCCGCAAGCGTTTAAGACGTTGCCAGCTAGTGAGATGGCAATCGAAGGTTTGAAGAACGTGGAGATTGATGTTGGTGGAGAGTTGTTGATTGGAGAATGTAGGATATGTTTGGATGAACTCATGAATGGAATGGAAGTTACGCGATTGCCGTGCGCTCATCTTTATCATAGAGACTGTATTGTTAAATGGCTGGAGACGAGTCATTTGTGCCCCTTGTGCCGGTACGCAATGCCGCTGAGCTGA

Coding sequence (CDS)

TCAAATCACTCTCAACAATTTCACTATCTCCTAAATTCCTCTCTATCTTCTTTCTTGATACCTTGCGATGTCTTTTTCGCTACTGCCTCCAGTATCCTCATCGCTGTTTTCTCTGGCACCGGTGTCGCACAAGACTTCTTGGACACGGCTCTGCCTGACGTTCTCTCTGTCGCCGCTGATATCGCATGCAATCCTCTTAATGCTGGCCGTACCATACTTCCGTTGGCGGTGTCCGTTTTAGTTGTTTCGCCCTACAATGAACGCCAGGAAATTGGGCGAGTCTTACGGGAATCATCCCCGCAAGCGTTTAAGACGTTGCCAGCTAGTGAGATGGCAATCGAAGGTTTGAAGAACGTGGAGATTGATGTTGGTGGAGAGTTGTTGATTGGAGAATGTAGGATATGTTTGGATGAACTCATGAATGGAATGGAAGTTACGCGATTGCCGTGCGCTCATCTTTATCATAGAGACTGTATTGTTAAATGGCTGGAGACGAGTCATTTGTGCCCCTTGTGCCGGTACGCAATGCCGCTGAGCTGA

Protein sequence

SNHSQQFHYLLNSSLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTALPDVLSVAADIACNPLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKNVEIDVGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS*

Homology

[Close]

BLAST of Cucsa.188140 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 5.5e-15
Identity = 47/137 (34.31%), Postives = 71/137 (51.82%), Query Frame = 1


Query: 46  FLDTALPDVLSVAADIAC----NPLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQ 105
           F+   L D+ S  A I      NP + G   LP  +    + P  E  ++ + L E+ P 
Sbjct: 136 FIQNHLNDLRSSGAQIEFVIQNNPSDQGFR-LPANIGDYFIGPGLE--QLIQQLAENDPN 195

Query: 106 AFKTLPASEMAIEGLKNVEIDVGG-ELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIV 165
            + T PAS+ AIE L  V I          +C +C+D+   G E  ++PC HLYH+DC++
Sbjct: 196 RYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLL 255

Query: 166 KWLETSHLCPLCRYAMP 178
            WLE  + CP+CR+ +P
Sbjct: 256 PWLELHNSCPVCRHELP 269

[Close]

BLAST of Cucsa.188140 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 6.1e-14
Identity = 32/92 (34.78%), Postives = 61/92 (66.30%), Query Frame = 1


Query: 89  QEIGRVLRESSPQAFKTLPASEMAIEGLKNVEIDVGGELL---IGECRICLDELMNGMEV 148
           +++ + L E+ P  + T PAS+ AI+ L  V++    ++L   + +C +C+DE  +G +V
Sbjct: 172 EQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTK--DMLKSEMNQCAVCMDEFEDGSDV 231

Query: 149 TRLPCAHLYHRDCIVKWLETSHLCPLCRYAMP 178
            ++PC H++H+DC++ WLE  + CP+CR+ +P
Sbjct: 232 KQMPCKHVFHQDCLLPWLELHNSCPVCRFELP 261

[Close]

BLAST of Cucsa.188140 vs. Swiss-Prot
Match: CIP8_ARATH (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana GN=CIP8 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-10
Identity = 28/70 (40.00%), Postives = 42/70 (60.00%), Query Frame = 1


Query: 107 PASEMAIEGLKNVEIDVGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETS 166
           PA++ AIE L+  E+      ++  C +C D ++ G    +LPC H YH DCIV WL T 
Sbjct: 232 PAAKSAIEALETFEVSSSEGEMVMVCAVCKDGMVMGETGKKLPCGHCYHGDCIVPWLGTR 291

Query: 167 HLCPLCRYAM 177
           + CP+CR+ +
Sbjct: 292 NSCPVCRFQL 301

[Close]

BLAST of Cucsa.188140 vs. Swiss-Prot
Match: RDUF1_ARATH (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-10
Identity = 29/71 (40.85%), Postives = 40/71 (56.34%), Query Frame = 1


Query: 107 PASEMAIEGLKNVEIDVGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETS 166
           PAS+ AIE L  VEI          C +C +    G+E   +PC H++H DCIV WL   
Sbjct: 190 PASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIR 249

Query: 167 HLCPLCRYAMP 178
           + CP+CR+ +P
Sbjct: 250 NSCPVCRFELP 260

[Close]

BLAST of Cucsa.188140 vs. Swiss-Prot
Match: RDUF2_ARATH (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana GN=DURF2 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.1e-10
Identity = 31/74 (41.89%), Postives = 41/74 (55.41%), Query Frame = 1


Query: 107 PASEMAIEGLKNVEID---VGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWL 166
           PAS+ AIE L  VEI    +G E     C +C +      E   +PC HL+H DCIV WL
Sbjct: 174 PASKSAIESLPRVEISDCHIGSE---ANCAVCTEIFETETEAREMPCKHLFHDDCIVPWL 233

Query: 167 ETSHLCPLCRYAMP 178
              + CP+CR+ +P
Sbjct: 234 SIRNSCPVCRFELP 244

[Close]

BLAST of Cucsa.188140 vs. TrEMBL
Match: A0A0A0KDU3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G385070 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 9.8e-96
Identity = 177/179 (98.88%), Postives = 178/179 (99.44%), Query Frame = 1


Query: 1   SNHSQQFHYLLNSSLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTALPDVLSVAAD 60
           SNHSQQFHYLLNSSLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTA+PDVLSVAAD
Sbjct: 48  SNHSQQFHYLLNSSLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAAD 107

Query: 61  IACNPLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKNVE 120
           IACNPLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLK VE
Sbjct: 108 IACNPLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKKVE 167

Query: 121 IDVGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 180
           IDVGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 168 IDVGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cucsa.188140 vs. TrEMBL
Match: M5XI10_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021670mg PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 3.2e-38
Identity = 81/173 (46.82%), Postives = 114/173 (65.90%), Query Frame = 1


Query: 5   QQFHYLLNSSLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTALPDVLSVAADIACN 64
           Q  +  LNS   SF +P DVFF+     +  V SG+GV+ DF+   LP+V S A D+A  
Sbjct: 46  QDLNIPLNSIQESFFVPFDVFFSHTQETIQTVLSGSGVSMDFVHVLLPEVHSFAMDVAQE 105

Query: 65  PLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKNVEIDVG 124
           P NAG  I+PL ++VL V+P+++R +I R + ES  Q F  +PA++ +I GLK V+ID  
Sbjct: 106 PANAGPRIVPLVLAVLAVTPFDDRDQIERAIMESG-QVFNPVPAAKSSIAGLKKVKID-- 165

Query: 125 GELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMP 178
              +  EC ICL+E   G+EV  +PC+HLYH+DCIV+WL+ SHLCP CR+ +P
Sbjct: 166 NLSVTKECSICLEEFSAGLEVVLMPCSHLYHKDCIVEWLKRSHLCPYCRFKLP 215

[Close]

BLAST of Cucsa.188140 vs. TrEMBL
Match: B9GU33_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0002s08360g PE=4 SV=2)

HSP 1 Score: 149.4 bits (376), Expect = 4.1e-33
Identity = 76/171 (44.44%), Postives = 112/171 (65.50%), Query Frame = 1


Query: 11  LNSSLSSFLIPCDVFFATAS--SILIAVFSGTGVAQDFLDTALPDVLSVAADIACNPLNA 70
           LN++  S L P  +F +  +  SI+  + S  G + DF+++A PD+ S A D+  NP NA
Sbjct: 44  LNTTNESHLFPRQLFSSHVNRESIVKEILSSMGCSSDFIESAAPDISSFALDMVTNPCNA 103

Query: 71  GRT-ILPLAVSVLVVSPYNERQEIGRVLRESSPQ-AFKTLPASEMAIEGLKNVEIDVGGE 130
             + +L + +++ V +PY+ER+EI R L ES  Q A +  PAS+  I+GLK + ++  G 
Sbjct: 104 SSSEVLTMVLAIHVTTPYDEREEIDRALSESLMQEASRFKPASKSCIDGLKRMSLE--GS 163

Query: 131 LLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMP 178
             + EC +CL+E + G EV  LPC H++H DCIV+WLETSHLCPLCR+AMP
Sbjct: 164 CSMKECMVCLEEFLMGSEVVCLPCGHIFHGDCIVRWLETSHLCPLCRFAMP 212

[Close]

BLAST of Cucsa.188140 vs. TrEMBL
Match: B9RJ35_RICCO (Zinc finger protein, putative OS=Ricinus communis GN=RCOM_1030640 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.9e-31
Identity = 75/186 (40.32%), Postives = 115/186 (61.83%), Query Frame = 1


Query: 2   NHSQQFHYLLNSSLS------SFLIPCDVFFAT----ASSILIAVFSGTGVAQDFLDTAL 61
           +H  + HY  ++ +       SF IP ++ F++     SSI+ A+ S T  ++DF+D  L
Sbjct: 35  HHVLEIHYPESNQIPVTTTHRSFPIPRNLIFSSDDQSRSSIMDAILSTTAASRDFIDLIL 94

Query: 62  PDVLSVAADIACNPLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEM 121
           P+V S A ++A +P N    +L + + + VV+PY+ER+EI R L E+  +  +  PAS+ 
Sbjct: 95  PEVSSFALEMATHPSNKNVKVLTMGLLICVVTPYDEREEIDRALGETIQEKARFKPASKS 154

Query: 122 AIEGLKNVEIDVGGELLIGE-CRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCP 177
            IEGLK V ID   + + GE C +CL++   G  V RL C H++H DCIVKWL T+HLCP
Sbjct: 155 CIEGLKRVRIDKDDDKISGETCMVCLEKESVGEVVIRLACGHVFHEDCIVKWLHTNHLCP 214

[Close]

BLAST of Cucsa.188140 vs. TrEMBL
Match: A0A0D2U3Q8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G153700 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.4e-25
Identity = 80/197 (40.61%), Postives = 109/197 (55.33%), Query Frame = 1


Query: 1   SNHSQQFHYLLNSSLSSFLIPCDVFFATAS--SILIAVFSGTGVAQDFLDTAL-PDVLSV 60
           S+H  Q    L +   S L   D+FF+  +  +ILIA+ +  G +Q F+DT L PDV+S 
Sbjct: 39  SSHDPQI--TLETRNRSILCRRDLFFSEQNGRNILIAMVADFGASQHFIDTVLVPDVMSF 98

Query: 61  AADIACNPLNAGRTILPLAVSVLV-VSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGL 120
           A D    P+N GR ++ L V +++ V P +E       + ES   +    PAS+ +IE L
Sbjct: 99  AWDTHSMPMNLGRQVIKLRVELVIEVRPNDE-------IEESLTSSVNFKPASKSSIEAL 158

Query: 121 KNV-----EID----------VGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVK 179
           K V     E+D            G     EC +CL+E ++G EV  LPC H+YH  CIVK
Sbjct: 159 KRVIWDDDELDHIPLKKRRKLAKGLSSRKECVVCLEEFLDGEEVASLPCGHVYHYGCIVK 218

[Close]

BLAST of Cucsa.188140 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 79.0 bits (193), Expect = 3.4e-15
Identity = 32/92 (34.78%), Postives = 61/92 (66.30%), Query Frame = 1


Query: 89  QEIGRVLRESSPQAFKTLPASEMAIEGLKNVEIDVGGELL---IGECRICLDELMNGMEV 148
           +++ + L E+ P  + T PAS+ AI+ L  V++    ++L   + +C +C+DE  +G +V
Sbjct: 172 EQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTK--DMLKSEMNQCAVCMDEFEDGSDV 231

Query: 149 TRLPCAHLYHRDCIVKWLETSHLCPLCRYAMP 178
            ++PC H++H+DC++ WLE  + CP+CR+ +P
Sbjct: 232 KQMPCKHVFHQDCLLPWLELHNSCPVCRFELP 261

[Close]

BLAST of Cucsa.188140 vs. TAIR10
Match: AT1G55530.1 (AT1G55530.1 RING/U-box superfamily protein)

HSP 1 Score: 75.1 bits (183), Expect = 4.9e-14
Identity = 34/83 (40.96%), Postives = 49/83 (59.04%), Query Frame = 1


Query: 95  LRESSPQAFKTLPASEMAIEGLKNVEIDVGGELLIGECRICLDELMNGMEVTRLPCAHLY 154
           L E+ P  + T PA + A+E L  V+I+   E L  +C +CLD+   G E   +PC H +
Sbjct: 191 LAENDPNRYGTPPAKKEAVEALATVKIE---ETL--QCSVCLDDFEIGTEAKLMPCTHKF 250

Query: 155 HRDCIVKWLETSHLCPLCRYAMP 178
           H DC++ WLE    CP+CRY +P
Sbjct: 251 HSDCLLPWLELHSSCPVCRYQLP 268

[Close]

BLAST of Cucsa.188140 vs. TAIR10
Match: AT3G13430.1 (AT3G13430.1 RING/U-box superfamily protein)

HSP 1 Score: 71.6 bits (174), Expect = 5.5e-13
Identity = 32/75 (42.67%), Postives = 47/75 (62.67%), Query Frame = 1


Query: 103 FKTLPASEMAIEGLKNVEIDVGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKW 162
           + T PA++ A+E L  V+I+    LL  +C +CLD+   GME   +PC H +H DC++ W
Sbjct: 200 YGTPPATKEAVEALAMVKIE--DSLL--QCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPW 259

Query: 163 LETSHLCPLCRYAMP 178
           LE    CP+CRY +P
Sbjct: 260 LELHSSCPVCRYLLP 270

[Close]

BLAST of Cucsa.188140 vs. TAIR10
Match: AT1G60360.1 (AT1G60360.1 RING/U-box superfamily protein)

HSP 1 Score: 70.9 bits (172), Expect = 9.3e-13
Identity = 31/73 (42.47%), Postives = 46/73 (63.01%), Query Frame = 1


Query: 107 PASEMAIEGLKNVEIDVGGELL-IGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLET 166
           PASE  I  L +V+I        + +C +C++E + G + T LPC H+YH+DCIV WL  
Sbjct: 198 PASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIVPWLRL 257

Query: 167 SHLCPLCRYAMPL 179
           ++ CP+CR  +PL
Sbjct: 258 NNSCPICRRDLPL 270

[Close]

BLAST of Cucsa.188140 vs. TAIR10
Match: AT3G02340.1 (AT3G02340.1 RING/U-box superfamily protein)

HSP 1 Score: 68.9 bits (167), Expect = 3.5e-12
Identity = 30/80 (37.50%), Postives = 47/80 (58.75%), Query Frame = 1


Query: 98  SSPQAFKTLPASEMAIEGLKNVEIDVGGELLIGECRICLDELMNGMEVTRLPCAHLYHRD 157
           ++    + LP  E+A+E     E+D G  +    C +C DE++   +V RLPC+H YH +
Sbjct: 310 AAKSVIQDLPVVELAVE-----ELDKGNNV----CAVCKDEMLVEEKVRRLPCSHFYHGE 369

Query: 158 CIVKWLETSHLCPLCRYAMP 178
           CI+ WL   + CP+CRY +P
Sbjct: 370 CIIPWLGIRNTCPVCRYELP 380

[Close]

BLAST of Cucsa.188140 vs. NCBI nr
Match: gi|449465461|ref|XP_004150446.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus])

HSP 1 Score: 357.5 bits (916), Expect = 1.4e-95
Identity = 177/179 (98.88%), Postives = 178/179 (99.44%), Query Frame = 1


Query: 1   SNHSQQFHYLLNSSLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTALPDVLSVAAD 60
           SNHSQQFHYLLNSSLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTA+PDVLSVAAD
Sbjct: 48  SNHSQQFHYLLNSSLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAAD 107

Query: 61  IACNPLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKNVE 120
           IACNPLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLK VE
Sbjct: 108 IACNPLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKKVE 167

Query: 121 IDVGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 180
           IDVGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 168 IDVGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cucsa.188140 vs. NCBI nr
Match: gi|659133409|ref|XP_008466716.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo])

HSP 1 Score: 345.1 bits (884), Expect = 7.3e-92
Identity = 170/179 (94.97%), Postives = 175/179 (97.77%), Query Frame = 1


Query: 1   SNHSQQFHYLLNSSLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTALPDVLSVAAD 60
           SNHSQQ HYLLNSSLSS+LIPCDVFFATASSILIAVFSGTGVAQDFLDTA+PDVLSVAAD
Sbjct: 48  SNHSQQSHYLLNSSLSSYLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAAD 107

Query: 61  IACNPLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKNVE 120
           IACNPLNAGRTILPL VSVLVVSPYNERQEIG+VLRESSPQAFKTLPASEMAIE LKNVE
Sbjct: 108 IACNPLNAGRTILPLTVSVLVVSPYNERQEIGQVLRESSPQAFKTLPASEMAIEDLKNVE 167

Query: 121 IDVGGELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 180
           I VGGELLIGECRICLDEL+NGME+TRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 168 IGVGGELLIGECRICLDELVNGMEITRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cucsa.188140 vs. NCBI nr
Match: gi|596235776|ref|XP_007224385.1| (hypothetical protein PRUPE_ppa021670mg [Prunus persica])

HSP 1 Score: 166.4 bits (420), Expect = 4.6e-38
Identity = 81/173 (46.82%), Postives = 114/173 (65.90%), Query Frame = 1


Query: 5   QQFHYLLNSSLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTALPDVLSVAADIACN 64
           Q  +  LNS   SF +P DVFF+     +  V SG+GV+ DF+   LP+V S A D+A  
Sbjct: 46  QDLNIPLNSIQESFFVPFDVFFSHTQETIQTVLSGSGVSMDFVHVLLPEVHSFAMDVAQE 105

Query: 65  PLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKNVEIDVG 124
           P NAG  I+PL ++VL V+P+++R +I R + ES  Q F  +PA++ +I GLK V+ID  
Sbjct: 106 PANAGPRIVPLVLAVLAVTPFDDRDQIERAIMESG-QVFNPVPAAKSSIAGLKKVKID-- 165

Query: 125 GELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMP 178
              +  EC ICL+E   G+EV  +PC+HLYH+DCIV+WL+ SHLCP CR+ +P
Sbjct: 166 NLSVTKECSICLEEFSAGLEVVLMPCSHLYHKDCIVEWLKRSHLCPYCRFKLP 215

[Close]

BLAST of Cucsa.188140 vs. NCBI nr
Match: gi|645225559|ref|XP_008219633.1| (PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Prunus mume])

HSP 1 Score: 166.0 bits (419), Expect = 6.0e-38
Identity = 80/173 (46.24%), Postives = 114/173 (65.90%), Query Frame = 1


Query: 5   QQFHYLLNSSLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTALPDVLSVAADIACN 64
           Q  +  LNS   SF +P DVFF+     +  V SG+GV+ DF+   LP+V S A D+A  
Sbjct: 46  QDLNIPLNSIQESFFVPFDVFFSHTQETIQTVLSGSGVSMDFVHVLLPEVHSFAMDVAQE 105

Query: 65  PLNAGRTILPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKNVEIDVG 124
           P NAG  ++PL ++VL V+P+++R +I R + ES  Q F  +PA++ +I GLK V+ID  
Sbjct: 106 PANAGPRVVPLVLAVLAVTPFDDRDQIERAIMESG-QVFNPVPAAKSSIAGLKKVKID-- 165

Query: 125 GELLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMP 178
              +  EC ICL+E   G+EV  +PC+HLYH+DCIV+WL+ SHLCP CR+ +P
Sbjct: 166 NLSVTKECSICLEEFSAGLEVVLMPCSHLYHKDCIVEWLKRSHLCPYCRFKLP 215

[Close]

BLAST of Cucsa.188140 vs. NCBI nr
Match: gi|566156911|ref|XP_002300984.2| (hypothetical protein POPTR_0002s08360g [Populus trichocarpa])

HSP 1 Score: 149.4 bits (376), Expect = 5.8e-33
Identity = 76/171 (44.44%), Postives = 112/171 (65.50%), Query Frame = 1


Query: 11  LNSSLSSFLIPCDVFFATAS--SILIAVFSGTGVAQDFLDTALPDVLSVAADIACNPLNA 70
           LN++  S L P  +F +  +  SI+  + S  G + DF+++A PD+ S A D+  NP NA
Sbjct: 44  LNTTNESHLFPRQLFSSHVNRESIVKEILSSMGCSSDFIESAAPDISSFALDMVTNPCNA 103

Query: 71  GRT-ILPLAVSVLVVSPYNERQEIGRVLRESSPQ-AFKTLPASEMAIEGLKNVEIDVGGE 130
             + +L + +++ V +PY+ER+EI R L ES  Q A +  PAS+  I+GLK + ++  G 
Sbjct: 104 SSSEVLTMVLAIHVTTPYDEREEIDRALSESLMQEASRFKPASKSCIDGLKRMSLE--GS 163

Query: 131 LLIGECRICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMP 178
             + EC +CL+E + G EV  LPC H++H DCIV+WLETSHLCPLCR+AMP
Sbjct: 164 CSMKECMVCLEEFLMGSEVVCLPCGHIFHGDCIVRWLETSHLCPLCRFAMP 212

The following BLAST results are available for this feature:

BLAST of Cucsa.188140 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RING1_GOSHI	5.5e-15	34.31	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1	[more]
RNG1L_ARATH	6.1e-14	34.78	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1...	[more]
CIP8_ARATH	1.1e-10	40.00	E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana GN=CIP8 PE=1 SV=1	[more]
RDUF1_ARATH	1.4e-10	40.85	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana GN=RDUF1 PE=1 SV=1	[more]
RDUF2_ARATH	3.1e-10	41.89	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana GN=DURF2 PE=1 SV=1	[more]

BLAST of Cucsa.188140 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KDU3_CUCSA	9.8e-96	98.88	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G385070 PE=4 SV=1	[more]
M5XI10_PRUPE	3.2e-38	46.82	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021670mg PE=4 SV=1	[more]
B9GU33_POPTR	4.1e-33	44.44	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0002s08360g PE=4 SV=2	[more]
B9RJ35_RICCO	2.9e-31	40.32	Zinc finger protein, putative OS=Ricinus communis GN=RCOM_1030640 PE=4 SV=1	[more]
A0A0D2U3Q8_GOSRA	1.4e-25	40.61	Uncharacterized protein OS=Gossypium raimondii GN=B456_008G153700 PE=4 SV=1	[more]

BLAST of Cucsa.188140 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G19950.1	3.4e-15	34.78	RING/U-box superfamily protein	[more]
AT1G55530.1	4.9e-14	40.96	RING/U-box superfamily protein	[more]
AT3G13430.1	5.5e-13	42.67	RING/U-box superfamily protein	[more]
AT1G60360.1	9.3e-13	42.47	RING/U-box superfamily protein	[more]
AT3G02340.1	3.5e-12	37.50	RING/U-box superfamily protein	[more]

BLAST of Cucsa.188140 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449465461\|ref\|XP_004150446.1\|	1.4e-95	98.88	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus]	[more]
gi\|659133409\|ref\|XP_008466716.1\|	7.3e-92	94.97	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo]	[more]
gi\|596235776\|ref\|XP_007224385.1\|	4.6e-38	46.82	hypothetical protein PRUPE_ppa021670mg [Prunus persica]	[more]
gi\|645225559\|ref\|XP_008219633.1\|	6.0e-38	46.24	PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Prunus mume]	[more]
gi\|566156911\|ref\|XP_002300984.2\|	5.8e-33	44.44	hypothetical protein POPTR_0002s08360g [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR011016	Znf_RING-CH
IPR013083	Znf_RING/FYVE/PHD

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.188140.1	Cucsa.188140.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 131..173 score: 8.2
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 132..172 score: 5.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 132..173 score: 12
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 131..173 score: 0.
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 131..175 score: 5.7
None	No IPR available	PANTHER	PTHR22763	RING ZINC FINGER PROTEIN	coord: 87..177 score: 4.7
None	No IPR available	PANTHER	PTHR22763:SF46	PROTEIN SAN1	coord: 87..177 score: 4.7
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 131..177 score: 3.95

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.188140	Cla013937	Watermelon (97103) v1	cgywmB365
Cucsa.188140	Csa6G385070	Cucumber (Chinese Long) v2	cgycuB289
Cucsa.188140	MELO3C010617	Melon (DHL92) v3.5.1	cgymeB341
Cucsa.188140	ClCG08G005710	Watermelon (Charleston Gray)	cgywcgB343
Cucsa.188140	CSPI06G20370	Wild cucumber (PI 183967)	cgycpiB298
Cucsa.188140	CmaCh15G006740	Cucurbita maxima (Rimu)	cgycmaB0546
Cucsa.188140	CmaCh04G023640	Cucurbita maxima (Rimu)	cgycmaB0547
Cucsa.188140	CmoCh04G024750	Cucurbita moschata (Rifu)	cgycmoB0548
Cucsa.188140	CmoCh15G006860	Cucurbita moschata (Rifu)	cgycmoB0547
Cucsa.188140	Lsi08G004370	Bottle gourd (USVL1VR-Ls)	cgylsiB320
Cucsa.188140	Cp4.1LG13g04620	Cucurbita pepo (Zucchini)	cgycpeB0527
Cucsa.188140	MELO3C010617.2	Melon (DHL92) v3.6.1	cgymedB336
Cucsa.188140	CsaV3_6G034420	Cucumber (Chinese Long) v3	cgycucB302
Cucsa.188140	Cla97C08G148490	Watermelon (97103) v2	cgywmbB349
Cucsa.188140	Bhi04G001515	Wax gourd	cgywgoB391
Cucsa.188140	Carg01534	Silver-seed gourd	carcgyB0949
Cucsa.188140	Carg24436	Silver-seed gourd	carcgyB0515

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.188140	Cucurbita pepo (Zucchini)	cgycpeB0528