BLAST of Cucsa.065570 vs. Swiss-Prot
Match:
PP285_ARATH (Pentatricopeptide repeat-containing protein At3g57430, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H81 PE=2 SV=2)
HSP 1 Score: 389.8 bits (1000), Expect = 6.1e-107
Identity = 237/685 (34.60%), Postives = 365/685 (53.28%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRL---NAIGNGVRIHS 87
WNS+I + L + M + P S T+ V+ AC L + G ++H+
Sbjct: 167 WNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHA 226
Query: 88 CIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKE 147
+N + T LV Y K G +A + + RDLV+WN ++S E
Sbjct: 227 YGLRKGELNSFIINT-LVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLE 286
Query: 148 AVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALV 207
A+ EM G+ P+ T+ ++L AC + LR G+E+H Y L+NG D +++VG+ALV
Sbjct: 287 ALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALV 346
Query: 208 GFYMRFDAVLS-HRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIE-GIKFDA 267
Y VLS RVF M R I WNA+I G+ +AL L+ M G+ ++
Sbjct: 347 DMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANS 406
Query: 268 VTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDER 327
TM V+ AC G ++IHGF +K GL+ + + +L +MY G
Sbjct: 407 TTMAGVVPACVRSGAFSRK--------EAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKID 466
Query: 328 AATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMIS------------ELEPNS 387
A +F + RDLV+WN++I+ Y+ +++ ALLL + M + L+PNS
Sbjct: 467 IAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNS 526
Query: 388 VTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIF 447
+T++ IL SC L+ L G+ +HAY + +L D ++ +A + MYA+CG LQ + K+F
Sbjct: 527 ITLMTILPSCAALSALAKGKEIHAYAIKN--NLATDVAVGSALVDMYAKCGCLQMSRKVF 586
Query: 448 CTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTV 507
+ +++++WN +I YGMHG G++A M+ G KPN V+F SV +ACSHSG+
Sbjct: 587 DQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVD 646
Query: 508 TGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIE-PDASIWRALLS 567
GL++F+ M D+G+ P HY C+VDLLGR G EA +N MP + A W +LL
Sbjct: 647 EGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMPRDFNKAGAWSSLLG 706
Query: 568 SCQIKSNNKLLETIFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKP 627
+ +I +N ++ E L++LEP+ +++LL+NIY++AGLW + ++R+ ++E+G+ K
Sbjct: 707 ASRIHNNLEIGEIAAQNLIQLEPNVASHYVLLANIYSSAGLWDKATEVRRNMKEQGVRKE 766
Query: 628 PGTSWIVIGNQVHHFTATDVLHPQSERIYENLNSLTSPDRYSLFLSDIS----YKRSTSC 687
PG SWI G++VH F A D HPQSE++ L +L R ++ D S
Sbjct: 767 PGCSWIEHGDEVHKFVAGDSSHPQSEKLSGYLETLWERMRKEGYVPDTSCVLHNVEEDEK 826
HSP 2 Score: 218.4 bits (555), Expect = 2.4e-55
Identity = 153/590 (25.93%), Postives = 285/590 (48.31%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
W +++ + + TY M LGI PD+ P +LKA L + G +IH+ +
Sbjct: 65 WIDLLRSKVRSNLLREAVLTYVDMIVLGIKPDNYAFPALLKAVADLQDMELGKQIHAHVY 124
Query: 88 GLDL-INDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAV 147
++ V V LV+ Y KCG KVF + ER+ VSWN+LIS ++ A+
Sbjct: 125 KFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMAL 184
Query: 148 LLFVEMKKAGLTPNSRTVVALLLACGEM---LELRLGQEIHGYCLRNGLFDMDAYVGTAL 207
F M + P+S T+V+++ AC + L +G+++H Y LR G ++++++ L
Sbjct: 185 EAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKG--ELNSFIINTL 244
Query: 208 VGFYMRFDAVLSHRV-FSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDA 267
V Y + + S +V R++V+WN +++ +AL+ M++EG++ D
Sbjct: 245 VAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDE 304
Query: 268 VTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNG-LEINTSLNTSLTEMYINCGDE 327
T+ V+ AC+ LR G K +H +A+KNG L+ N+ + ++L +MY NC
Sbjct: 305 FTISSVLPACSHLEMLRTG--------KELHAYALKNGSLDENSFVGSALVDMYCNCKQV 364
Query: 328 RAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHM--ISELEPNSVTIVNILTS 387
+ +F R + WN++I+ Y +N++ +ALLLF M + L NS T+ ++ +
Sbjct: 365 LSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPA 424
Query: 388 CTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIV 447
C + + +H + +R L+ D + N + MY+R GK+ A +IF ++ R +V
Sbjct: 425 CVRSGAFSRKEAIHGFVVKR--GLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLV 484
Query: 448 SWNAMITGYGMHGRGRDATLAFAQMLD-----------DGFKPNNVSFASVLSACSHSGL 507
+WN MITGY DA L +M + KPN+++ ++L +C+
Sbjct: 485 TWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSA 544
Query: 508 TVTGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALL 567
G ++ H+ +A + +VD+ + G + + +P + + W ++
Sbjct: 545 LAKGKEI-HAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIP-QKNVITWNVII 604
Query: 568 SSCQIKSN-NKLLETIFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQI 598
+ + N + ++ + +V+ N FI + + +G+ E ++I
Sbjct: 605 MAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRI 640
BLAST of Cucsa.065570 vs. Swiss-Prot
Match:
PPR32_ARATH (Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H40 PE=2 SV=1)
HSP 1 Score: 386.0 bits (990), Expect = 8.9e-106
Identity = 221/619 (35.70%), Postives = 342/619 (55.25%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
+++++K K+ + L + +M + P +LK CG + G IH +
Sbjct: 103 YHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLV 162
Query: 88 GLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAVL 147
D+ T L + Y KC V EA KVF MPERDLVSWN +++GY + A+
Sbjct: 163 KSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALE 222
Query: 148 LFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGFY 207
+ M + L P+ T+V++L A + + +G+EIHGY +R+G FD + TALV Y
Sbjct: 223 MVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSG-FDSLVNISTALVDMY 282
Query: 208 MRFDAVLSHR-VFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTML 267
+ ++ + R +F ML RN+VSWN++I ++ + +A+ ++ ML EG+K V+++
Sbjct: 283 AKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVM 342
Query: 268 VVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAATN 327
+ ACA+ G L G +H+L+++ GL+ N S+ SL MY C + A +
Sbjct: 343 GALHACADLGDLERGRFIHKLSVEL--------GLDRNVSVVNSLISMYCKCKEVDTAAS 402
Query: 328 MFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISE-LEPNSVTIVNILTSCTQLAH 387
MF + R LVSWN++I + +N AL F+ M S ++P++ T V+++T+ +L+
Sbjct: 403 MFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSI 462
Query: 388 LPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMI 447
+ +H R L+ + + A + MYA+CG + A IF + R + +WNAMI
Sbjct: 463 THHAKWIHGVVMRS--CLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMI 522
Query: 448 TGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGI 507
GYG HG G+ A F +M KPN V+F SV+SACSHSGL GL+ F+ M ++ I
Sbjct: 523 DGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKENYSI 582
Query: 508 APQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETIFG 567
+ HYG MVDLLGR G +EA FI MP++P +++ A+L +CQI N E
Sbjct: 583 ELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEKAAE 642
Query: 568 KLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVHHFT 627
+L EL P + G +LL+NIY AA +W +V Q+R + +GL K PG S + I N+VH F
Sbjct: 643 RLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGCSMVEIKNEVHSFF 702
Query: 628 ATDVLHPQSERIYENLNSL 645
+ HP S++IY L L
Sbjct: 703 SGSTAHPDSKKIYAFLEKL 710
HSP 2 Score: 173.3 bits (438), Expect = 9.0e-42
Identity = 120/438 (27.40%), Postives = 212/438 (48.40%), Query Frame = 1
Query: 167 LLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAV-LSHRVFSLMLVR 226
LL C + ELR +I +NGL+ + + T LV + R+ +V + RVF + +
Sbjct: 43 LLERCSSLKELR---QILPLVFKNGLYQ-EHFFQTKLVSLFCRYGSVDEAARVFEPIDSK 102
Query: 227 NIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLH 286
V ++ ++ GF V D KAL+ + M + ++ +++ C + LR+G
Sbjct: 103 LNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVG---- 162
Query: 287 QLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISS 346
K IHG +K+G ++ T L MY C A +F R P+RDLVSWN++++
Sbjct: 163 ----KEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAG 222
Query: 347 YIKNDNAGKALLLFNHMISE-LEPNSVTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLE 406
Y +N A AL + M E L+P+ +TIV++L + + L + +G+ +H Y R +
Sbjct: 223 YSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRS--GFD 282
Query: 407 MDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQM 466
+++ A + MYA+CG L+ A ++F + R++VSWN+MI Y + ++A L F +M
Sbjct: 283 SLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKM 342
Query: 467 LDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGH 526
LD+G KP +VS L AC+ G G + H + + G+ ++ ++ + +
Sbjct: 343 LDEGVKPTDVSVMGALHACADLGDLERG-RFIHKLSVELGLDRNVSVVNSLISMYCKCKE 402
Query: 527 FSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETI-FGKLVELEPSNPGNFILLSN 586
A + + S W A++ N + ++ + + + P F +S
Sbjct: 403 VDTAASMFGKLQSRTLVS-WNAMILG--FAQNGRPIDALNYFSQMRSRTVKPDTFTYVSV 460
Query: 587 IYAAAGLWSEVVQIRKWL 602
I A A L + KW+
Sbjct: 463 ITAIAEL--SITHHAKWI 460
BLAST of Cucsa.065570 vs. Swiss-Prot
Match:
PP320_ARATH (Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis thaliana GN=DOT4 PE=2 SV=1)
HSP 1 Score: 385.2 bits (988), Expect = 1.5e-105
Identity = 217/612 (35.46%), Postives = 334/612 (54.58%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
WN ++ K + + + +M S G+ DS T V K+ L ++ G ++H I
Sbjct: 163 WNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFIL 222
Query: 88 GLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAVL 147
VG +LV FY K V A KVF EM ERD++SWN++I+GYV ++ +
Sbjct: 223 KSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLS 282
Query: 148 LFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGFY 207
+FV+M +G+ + T+V++ C + + LG+ +H ++ D + T L +
Sbjct: 283 VFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYS 342
Query: 208 MRFDAVLSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTMLV 267
D + VF M R++VS+ ++I G+ G +A+KL+ M EGI D T+
Sbjct: 343 KCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTA 402
Query: 268 VIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAATNM 327
V+ CA Y L G K +H + +N L + ++ +L +MY CG + A +
Sbjct: 403 VLNCCARYRLLDEG--------KRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELV 462
Query: 328 FTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISE--LEPNSVTIVNILTSCTQLAH 387
F+ +D++SWN++I Y KN A +AL LFN ++ E P+ T+ +L +C L+
Sbjct: 463 FSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSA 522
Query: 388 LPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMI 447
G+ +H Y R D +AN+ + MYA+CG L A +F + ++ +VSW MI
Sbjct: 523 FDKGREIHGYIMRN--GYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMI 582
Query: 448 TGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGI 507
GYGMHG G++A F QM G + + +SF S+L ACSHSGL G + F+ M + I
Sbjct: 583 AGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKI 642
Query: 508 APQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETIFG 567
P + HY C+VD+L R G +A FI +MPI PDA+IW ALL C+I + KL E +
Sbjct: 643 EPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPDATIWGALLCGCRIHHDVKLAEKVAE 702
Query: 568 KLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVHHFT 627
K+ ELEP N G ++L++NIYA A W +V ++RK + +RGL K PG SWI I +V+ F
Sbjct: 703 KVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIEIKGRVNIFV 762
Query: 628 ATDVLHPQSERI 638
A D +P++E I
Sbjct: 763 AGDSSNPETENI 764
HSP 2 Score: 216.5 bits (550), Expect = 9.3e-55
Identity = 133/447 (29.75%), Postives = 235/447 (52.57%), Query Frame = 1
Query: 59 DSATMPLVLKACGRLNAIGNGVRIHSCIRGLDLINDVRVGTALVDFYCKCGLVAEASKVF 118
D T+ VL+ C ++ +G + + IRG + D +G+ L Y CG + EAS+VF
Sbjct: 93 DPRTLCSVLQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVF 152
Query: 119 VEMPERDLVSWNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELR 178
E+ + WN L++ + ++ LF +M +G+ +S T + + + +
Sbjct: 153 DEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVH 212
Query: 179 LGQEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAVLSHR-VFSLMLVRNIVSWNAIITGF 238
G+++HG+ L++G + ++ VG +LV FY++ V S R VF M R+++SWN+II G+
Sbjct: 213 GGEQLHGFILKSGFGERNS-VGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGY 272
Query: 239 LNVGDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAI 298
++ G K L ++ ML+ GI+ D T++ V CA+ + LG +H + +K+
Sbjct: 273 VSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFS--- 332
Query: 299 KNGLEINTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALL 358
+ NT L +MY CGD +A +F R +VS+ S+I+ Y + AG+A+
Sbjct: 333 REDRFCNT-----LLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVK 392
Query: 359 LFNHMISE-LEPNSVTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITM 418
LF M E + P+ T+ +L C + L G+ +H + +E L D ++NA + M
Sbjct: 393 LFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWI--KENDLGFDIFVSNALMDM 452
Query: 419 YARCGKLQYAEKIFCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDD-GFKPNNVS 478
YA+CG +Q AE +F ++ + I+SWN +I GY + +A F +L++ F P+ +
Sbjct: 453 YAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERT 512
Query: 479 FASVLSACSHSGLTVTGLQLFHSMVRD 503
A VL AC+ G ++ ++R+
Sbjct: 513 VACVLPACASLSAFDKGREIHGYIMRN 528
HSP 3 Score: 162.9 bits (411), Expect = 1.2e-38
Identity = 114/450 (25.33%), Postives = 210/450 (46.67%), Query Frame = 1
Query: 143 KEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTA 202
+ AV L K + P RT+ ++L C + L+ G+E+ + NG F +D+ +G+
Sbjct: 78 ENAVKLLCVSGKWDIDP--RTLCSVLQLCADSKSLKDGKEVDNFIRGNG-FVIDSNLGSK 137
Query: 203 LVGFYMRF-DAVLSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFD 262
L Y D + RVF + + + WN ++ GD + ++ L+ M+ G++ D
Sbjct: 138 LSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMD 197
Query: 263 AVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDE 322
+ T V ++ + + G QLH GF +K+G S+ SL Y+
Sbjct: 198 SYTFSCVSKSFSSLRSVHGGEQLH--------GFILKSGFGERNSVGNSLVAFYLKNQRV 257
Query: 323 RAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHM-ISELEPNSVTIVNILTSC 382
+A +F +RD++SWNS+I+ Y+ N A K L +F M +S +E + TIV++ C
Sbjct: 258 DSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGC 317
Query: 383 TQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVS 442
+ LG+ +H+ + S E N + MY++CG L A+ +F + RS+VS
Sbjct: 318 ADSRLISLGRAVHSIGVKACFSRE--DRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVS 377
Query: 443 WNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMV 502
+ +MI GY G +A F +M ++G P+ + +VL+ C+ L G ++ H +
Sbjct: 378 YTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRV-HEWI 437
Query: 503 RDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLL 562
++ + + ++D+ + G EA + M ++ D W ++
Sbjct: 438 KENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVK-DIISWNTIIGGYSKNCYANEA 497
Query: 563 ETIFGKLVELEPSNPGNFILLSNIYAAAGL 591
++F L+E + +P + + A A L
Sbjct: 498 LSLFNLLLEEKRFSPDERTVACVLPACASL 512
BLAST of Cucsa.065570 vs. Swiss-Prot
Match:
PP398_ARATH (Pentatricopeptide repeat-containing protein At5g27110 OS=Arabidopsis thaliana GN=PCMP-E14 PE=2 SV=2)
HSP 1 Score: 380.9 bits (977), Expect = 2.8e-104
Identity = 219/622 (35.21%), Postives = 349/622 (56.11%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGI-TPDSATMPLVLKACGRLNAIGNGVRIHSCI 87
WNS++ ++K H L + ++ + I PDS T P V+KA G L G IH+ +
Sbjct: 74 WNSLMSGYSKNSMFHDTLEVFKRLLNCSICVPDSFTFPNVIKAYGALGREFLGRMIHTLV 133
Query: 88 RGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAV 147
+ DV V ++LV Y K L + +VF EMPERD+ SWN +IS + ++A+
Sbjct: 134 VKSGYVCDVVVASSLVGMYAKFNLFENSLQVFDEMPERDVASWNTVISCFYQSGEAEKAL 193
Query: 148 LLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGF 207
LF M+ +G PNS ++ + AC +L L G+EIH C++ G F++D YV +ALV
Sbjct: 194 ELFGRMESSGFEPNSVSLTVAISACSRLLWLERGKEIHRKCVKKG-FELDEYVNSALVDM 253
Query: 208 YMRFDAV-LSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 267
Y + D + ++ VF M +++V+WN++I G++ GD +++ + M+IEG + T+
Sbjct: 254 YGKCDCLEVAREVFQKMPRKSLVAWNSMIKGYVAKGDSKSCVEILNRMIIEGTRPSQTTL 313
Query: 268 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 327
++ AC+ L G K IHG+ I++ + + +N SL ++Y CG+ A
Sbjct: 314 TSILMACSRSRNLLHG--------KFIHGYVIRSVVNADIYVNCSLIDLYFKCGEANLAE 373
Query: 328 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISE-LEPNSVTIVNILTSCTQLA 387
+F++ + SWN +ISSYI N KA+ +++ M+S ++P+ VT ++L +C+QLA
Sbjct: 374 TVFSKTQKDVAESWNVMISSYISVGNWFKAVEVYDQMVSVGVKPDVVTFTSVLPACSQLA 433
Query: 388 HLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAM 447
L G+ +H + E LE D L +A + MY++CG + A +IF ++ + +VSW M
Sbjct: 434 ALEKGKQIHLSIS--ESRLETDELLLSALLDMYSKCGNEKEAFRIFNSIPKKDVVSWTVM 493
Query: 448 ITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFG 507
I+ YG HG+ R+A F +M G KP+ V+ +VLSAC H+GL GL+ F M +G
Sbjct: 494 ISAYGSHGQPREALYQFDEMQKFGLKPDGVTLLAVLSACGHAGLIDEGLKFFSQMRSKYG 553
Query: 508 IAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPD-ASIWRALLSSCQIKSNNKLLETI 567
I P + HY CM+D+LGR G EA I P D A + L S+C + + L + I
Sbjct: 554 IEPIIEHYSCMIDILGRAGRLLEAYEIIQQTPETSDNAELLSTLFSACCLHLEHSLGDRI 613
Query: 568 FGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVHH 627
LVE P + +++L N+YA+ W ++R ++E GL K PG SWI + ++V H
Sbjct: 614 ARLLVENYPDDASTYMVLFNLYASGESWDAARRVRLKMKEMGLRKKPGCSWIEMSDKVCH 673
Query: 628 FTATDVLHPQSERIYENLNSLT 646
F A D H ++E +YE L L+
Sbjct: 674 FFAEDRSHLRAENVYECLALLS 684
HSP 2 Score: 209.5 bits (532), Expect = 1.1e-52
Identity = 140/473 (29.60%), Postives = 236/473 (49.89%), Query Frame = 1
Query: 82 IHSCIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPER-DLVSWNALISGYVGCL 141
+H I L L DV + +L++ Y C A VF R D+ WN+L+SGY
Sbjct: 26 VHQRILTLGLRRDVVLCKSLINVYFTCKDHCSARHVFENFDIRSDVYIWNSLMSGYSKNS 85
Query: 142 CYKEAVLLFVEMKKAGL-TPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYV 201
+ + + +F + + P+S T ++ A G + LG+ IH +++G + D V
Sbjct: 86 MFHDTLEVFKRLLNCSICVPDSFTFPNVIKAYGALGREFLGRMIHTLVVKSG-YVCDVVV 145
Query: 202 GTALVGFYMRFDAVL-SHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGI 261
++LVG Y +F+ S +VF M R++ SWN +I+ F G+ KAL+L+ M G
Sbjct: 146 ASSLVGMYAKFNLFENSLQVFDEMPERDVASWNTVISCFYQSGEAEKALELFGRMESSGF 205
Query: 262 KFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINC 321
+ ++V++ V I AC+ L G K IH +K G E++ +N++L +MY C
Sbjct: 206 EPNSVSLTVAISACSRLLWLERG--------KEIHRKCVKKGFELDEYVNSALVDMYGKC 265
Query: 322 GDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISE-LEPNSVTIVNIL 381
A +F + P++ LV+WNS+I Y+ ++ + + N MI E P+ T+ +IL
Sbjct: 266 DCLEVAREVFQKMPRKSLVAWNSMIKGYVAKGDSKSCVEILNRMIIEGTRPSQTTLTSIL 325
Query: 382 TSCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRS 441
+C++ +L G+ +H Y R V+ ++ + + I +Y +CG+ AE +F Q
Sbjct: 326 MACSRSRNLLHGKFIHGYVIRSVVNADIYVNC--SLIDLYFKCGEANLAETVFSKTQKDV 385
Query: 442 IVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFH 501
SWN MI+ Y G A + QM+ G KP+ V+F SVL ACS G Q+ H
Sbjct: 386 AESWNVMISSYISVGNWFKAVEVYDQMVSVGVKPDVVTFTSVLPACSQLAALEKGKQI-H 445
Query: 502 SMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSS 551
+ + + ++D+ + G+ EA NS+P + D W ++S+
Sbjct: 446 LSISESRLETDELLLSALLDMYSKCGNEKEAFRIFNSIP-KKDVVSWTVMISA 485
BLAST of Cucsa.065570 vs. Swiss-Prot
Match:
PP357_ARATH (Pentatricopeptide repeat-containing protein At4g39530 OS=Arabidopsis thaliana GN=PCMP-E52 PE=3 SV=1)
HSP 1 Score: 380.6 bits (976), Expect = 3.7e-104
Identity = 213/622 (34.24%), Postives = 337/622 (54.18%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
W ++I K+ + L + Q+ + PD + VL AC L + G +IH+ I
Sbjct: 217 WTTMISGCVKMGRSYVSLQLFYQLMEDNVVPDGYILSTVLSACSILPFLEGGKQIHAHIL 276
Query: 88 GLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAVL 147
L D + L+D Y KCG V A K+F MP ++++SW L+SGY +KEA+
Sbjct: 277 RYGLEMDASLMNVLIDSYVKCGRVIAAHKLFNGMPNKNIISWTTLLSGYKQNALHKEAME 336
Query: 148 LFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGFY 207
LF M K GL P+ ++L +C + L G ++H Y ++ L + D+YV +L+ Y
Sbjct: 337 LFTSMSKFGLKPDMYACSSILTSCASLHALGFGTQVHAYTIKANLGN-DSYVTNSLIDMY 396
Query: 208 MRFDAVLSHR-VFSLMLVRNIVSWNAIITGFLNVG---DCAKALKLYSSMLIEGIKFDAV 267
+ D + R VF + ++V +NA+I G+ +G + +AL ++ M I+ +
Sbjct: 397 AKCDCLTDARKVFDIFAAADVVLFNAMIEGYSRLGTQWELHEALNIFRDMRFRLIRPSLL 456
Query: 268 TMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERA 327
T + +++A A L L K IHG K GL ++ ++L ++Y NC +
Sbjct: 457 TFVSLLRASASLTSLGLS--------KQIHGLMFKYGLNLDIFAGSALIDVYSNCYCLKD 516
Query: 328 ATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHM-ISELEPNSVTIVNILTSCTQ 387
+ +F +DLV WNS+ + Y++ +AL LF + +S P+ T N++T+
Sbjct: 517 SRLVFDEMKVKDLVIWNSMFAGYVQQSENEEALNLFLELQLSRERPDEFTFANMVTAAGN 576
Query: 388 LAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWN 447
LA + LGQ H +R LE + + NA + MYA+CG + A K F + +R +V WN
Sbjct: 577 LASVQLGQEFHCQLLKR--GLECNPYITNALLDMYAKCGSPEDAHKAFDSAASRDVVCWN 636
Query: 448 AMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRD 507
++I+ Y HG G+ A +M+ +G +PN ++F VLSACSH+GL GL+ F M+R
Sbjct: 637 SVISSYANHGEGKKALQMLEKMMSEGIEPNYITFVGVLSACSHAGLVEDGLKQFELMLR- 696
Query: 508 FGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLET 567
FGI P+ HY CMV LLGR G ++A I MP +P A +WR+LLS C N +L E
Sbjct: 697 FGIEPETEHYVCMVSLLGRAGRLNKARELIEKMPTKPAAIVWRSLLSGCAKAGNVELAEH 756
Query: 568 IFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVH 627
+ +P + G+F +LSNIYA+ G+W+E ++R+ ++ G+ K PG SWI I +VH
Sbjct: 757 AAEMAILSDPKDSGSFTMLSNIYASKGMWTEAKKVRERMKVEGVVKEPGRSWIGINKEVH 816
Query: 628 HFTATDVLHPQSERIYENLNSL 645
F + D H ++ +IYE L+ L
Sbjct: 817 IFLSKDKSHCKANQIYEVLDDL 826
HSP 2 Score: 198.0 bits (502), Expect = 3.4e-49
Identity = 152/538 (28.25%), Postives = 267/538 (49.63%), Query Frame = 1
Query: 65 LVLKACGRLNAIGNGVRIHSCIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPER 124
L L+A L N V + GL+L D + L++ Y + G + A KVF +MPER
Sbjct: 51 LQLRASDDLLHYQNVVHGQIIVWGLEL--DTYLSNILINLYSRAGGMVYARKVFEKMPER 110
Query: 125 DLVSWNALISGYVGCLCYKEAVLLFVEMKKAGL-TPNSRTVVALLLACGEMLELR---LG 184
+LVSW+ ++S Y+E++++F+E + +PN + + + AC L+ R +
Sbjct: 111 NLVSWSTMVSACNHHGIYEESLVVFLEFWRTRKDSPNEYILSSFIQACSG-LDGRGRWMV 170
Query: 185 QEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAV-LSHRVFSLMLVRNIVSWNAIITGFLN 244
++ + +++G FD D YVGT L+ FY++ + + VF + ++ V+W +I+G +
Sbjct: 171 FQLQSFLVKSG-FDRDVYVGTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMISGCVK 230
Query: 245 VGDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKN 304
+G +L+L+ ++ + + D + V+ AC+ L G K IH ++
Sbjct: 231 MGRSYVSLQLFYQLMEDNVVPDGYILSTVLSACSILPFLEGG--------KQIHAHILRY 290
Query: 305 GLEINTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLF 364
GLE++ SL L + Y+ CG AA +F P ++++SW +L+S Y +N +A+ LF
Sbjct: 291 GLEMDASLMNVLIDSYVKCGRVIAAHKLFNGMPNKNIISWTTLLSGYKQNALHKEAMELF 350
Query: 365 NHMIS-ELEPNSVTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYA 424
M L+P+ +ILTSC L L G +HAYT + +L D+ + N+ I MYA
Sbjct: 351 TSMSKFGLKPDMYACSSILTSCASLHALGFGTQVHAYTIK--ANLGNDSYVTNSLIDMYA 410
Query: 425 RCGKLQYAEKIFCTLQTRSIVSWNAMITGY---GMHGRGRDATLAFAQMLDDGFKPNNVS 484
+C L A K+F +V +NAMI GY G +A F M +P+ ++
Sbjct: 411 KCDCLTDARKVFDIFAAADVVLFNAMIEGYSRLGTQWELHEALNIFRDMRFRLIRPSLLT 470
Query: 485 FASVLSACSHSGLTVTGL-QLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINS 544
F S+L A + LT GL + H ++ +G+ + ++D+ ++ +
Sbjct: 471 FVSLLRA--SASLTSLGLSKQIHGLMFKYGLNLDIFAGSALIDVYSNCYCLKDSRLVFDE 530
Query: 545 MPIEPDASIWRALLSSCQIKSNNKLLETIFGKLVELEPSNPGNFILLSNIYAAAGLWS 593
M ++ D IW ++ + +S N+ +F +L +L P F + + AA L S
Sbjct: 531 MKVK-DLVIWNSMFAGYVQQSENEEALNLFLEL-QLSRERPDEFTFANMVTAAGNLAS 570
HSP 3 Score: 176.4 bits (446), Expect = 1.1e-42
Identity = 139/542 (25.65%), Postives = 258/542 (47.60%), Query Frame = 1
Query: 57 TPDSATMPLVLKACGRLNAIGNGV--RIHSCIRGLDLINDVRVGTALVDFYCKCGLVAEA 116
+P+ + ++AC L+ G + ++ S + DV VGT L+DFY K G + A
Sbjct: 143 SPNEYILSSFIQACSGLDGRGRWMVFQLQSFLVKSGFDRDVYVGTLLIDFYLKDGNIDYA 202
Query: 117 SKVFVEMPERDLVSWNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEM 176
VF +PE+ V+W +ISG V ++ LF ++ + + P+ + +L AC +
Sbjct: 203 RLVFDALPEKSTVTWTTMISGCVKMGRSYVSLQLFYQLMEDNVVPDGYILSTVLSACSIL 262
Query: 177 LELRLGQEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAVL-SHRVFSLMLVRNIVSWNAI 236
L G++IH + LR GL +MDA + L+ Y++ V+ +H++F+ M +NI+SW +
Sbjct: 263 PFLEGGKQIHAHILRYGL-EMDASLMNVLIDSYVKCGRVIAAHKLFNGMPNKNIISWTTL 322
Query: 237 ITGFLNVGDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIH 296
++G+ +A++L++SM G+K D ++ +CA L G Q +H
Sbjct: 323 LSGYKQNALHKEAMELFTSMSKFGLKPDMYACSSILTSCASLHALGFGTQ--------VH 382
Query: 297 GFAIKNGLEINTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIK---ND 356
+ IK L ++ + SL +MY C A +F D+V +N++I Y +
Sbjct: 383 AYTIKANLGNDSYVTNSLIDMYAKCDCLTDARKVFDIFAAADVVLFNAMIEGYSRLGTQW 442
Query: 357 NAGKALLLFNHMISEL-EPNSVTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLEMDASL 416
+AL +F M L P+ +T V++L + L L L + +H + L +D
Sbjct: 443 ELHEALNIFRDMRFRLIRPSLLTFVSLLRASASLTSLGLSKQIHGLMFK--YGLNLDIFA 502
Query: 417 ANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDDGF 476
+A I +Y+ C L+ + +F ++ + +V WN+M GY +A F ++
Sbjct: 503 GSALIDVYSNCYCLKDSRLVFDEMKVKDLVIWNSMFAGYVQQSENEEALNLFLELQLSRE 562
Query: 477 KPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGIA--PQLTHYGCMVDLLGRGGHFSE 536
+P+ +FA++++A + G Q FH + G+ P +T+ ++D+ + G +
Sbjct: 563 RPDEFTFANMVTAAGNLASVQLG-QEFHCQLLKRGLECNPYITN--ALLDMYAKCGSPED 622
Query: 537 AIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETIFGKLVE--LEPSNPGNFILLSNIY 588
A +S D W +++SS K + K++ +EP N+I +
Sbjct: 623 AHKAFDS-AASRDVVCWNSVISSYANHGEGKKALQMLEKMMSEGIEP----NYITFVGVL 665
BLAST of Cucsa.065570 vs. TrEMBL
Match:
A0A059D8T5_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B03685 PE=4 SV=1)
HSP 1 Score: 679.5 bits (1752), Expect = 4.3e-192
Identity = 350/642 (54.52%), Postives = 450/642 (70.09%), Query Frame = 1
Query: 26 KDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSC 85
KDWN++I+ H KL+ND AILSTY +M S ++PD A +PLVLKAC RL+ + G RIHS
Sbjct: 26 KDWNALIRRHAKLRNDGAILSTYARMLSSRVSPDEAALPLVLKACSRLDDVELGKRIHSS 85
Query: 86 IRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEA 145
I G DL+ DVRV T+LVDFY KCGLV +A VF M ERD+V WNA+I+GYVG ++EA
Sbjct: 86 ILGTDLMEDVRVATSLVDFYRKCGLVEDARYVFDRMAERDVVMWNAMINGYVGVGDHEEA 145
Query: 146 VLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVG 205
+ +FVEM + + P+SRTVVALLLACGE +ELR+GQEIH Y LR+GLF +VGTAL+G
Sbjct: 146 ISVFVEMGRRSVRPSSRTVVALLLACGEAMELRMGQEIHCYSLRSGLFHECPHVGTALIG 205
Query: 206 FYMRFDAVLSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 265
FY +F+ + H VF +M V+N+VSWNAIITG+ G+ L+L+ +L EG K D VTM
Sbjct: 206 FYAKFNLKVLHSVFDMMEVKNMVSWNAIITGYFEAGEYLMILELFVRILEEGGKLDEVTM 265
Query: 266 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 325
L+VIQAC EYG L L MQ+HQ+A+KS L + +L MY A
Sbjct: 266 LIVIQACTEYGSLGLAMQIHQIALKS--------DLSRDVYSANALLHMYSKLQKPEFAY 325
Query: 326 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMI---SELEPNSVTIVNILTSCTQ 385
+F P RD+ WNS++S+Y +A LF M+ SE++PNS TI++IL S
Sbjct: 326 EIFKSVPTRDVALWNSMLSTYNDFSCPEEAQRLFLRMLTEDSEIKPNSHTIISILASYDD 385
Query: 386 LAHLPLGQ-----CLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRS 445
L +G+ C+HAY TRRE S+ DASLANA ITMYA+CG L AEK+F + R
Sbjct: 386 APSLNIGRSIHGYCIHAYITRRESSIGSDASLANALITMYAKCGSLHNAEKVFNLIWRRD 445
Query: 446 IVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFH 505
++SWNA+ITGYG+HG+G A F +ML+DG KPN+ +F S+LSACSHSGL GL LF
Sbjct: 446 VISWNALITGYGIHGQGEGAIPTFLKMLEDGMKPNSATFVSILSACSHSGLIEEGLLLFS 505
Query: 506 SMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNN 565
SM F + P+L HYGC+VDLLGRGG+ EA+ FINSMPIE DAS+WRALLS+CQ+ SN
Sbjct: 506 SMFCKFKVNPELIHYGCVVDLLGRGGYLGEALDFINSMPIEADASVWRALLSACQVHSNT 565
Query: 566 KLLETIFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVI 625
KL++ +F K+VELEP N GN++LLSNIYA AGLWSEV IR WL+E+GL KPPG SWIVI
Sbjct: 566 KLVKLVFEKIVELEPMNEGNYVLLSNIYATAGLWSEVGLIRAWLKEKGLRKPPGISWIVI 625
Query: 626 GNQVHHFTATDVLHPQSERIYENLNSLTSPDRYSLFLSDISY 660
+Q H FTA D HP+++ IY L+SL+ + + ++SDI +
Sbjct: 626 RDQAHVFTARDQSHPRTDLIYAILSSLSCSIKDAGYVSDIQW 659
BLAST of Cucsa.065570 vs. TrEMBL
Match:
A0A061GS93_THECC (Pentatricopeptide repeat-containing protein OS=Theobroma cacao GN=TCM_040700 PE=4 SV=1)
HSP 1 Score: 591.3 bits (1523), Expect = 1.5e-165
Identity = 307/651 (47.16%), Postives = 424/651 (65.13%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
WN++IK + + L + +M G+ DS TM +++AC ++ G +IH
Sbjct: 228 WNAMIKGYFDIGESLKALKLFEKMLMDGVEFDSVTMLALIQACAEFGSLELGSQIHQMAI 287
Query: 88 GLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAVL 147
ND+ + AL++ Y G + A K+F P RD+ WN++IS Y C +EA
Sbjct: 288 KCSYSNDLFIVNALLNMYADIGSLKSACKLFDVTPRRDVALWNSMISAYFEYSCNEEATS 347
Query: 148 LFVEMKKAGLTPNSRTVVALLLACGEMLE-LRLGQEIHGYCLRNGLFDMDAYVGTALVGF 207
LFV M+ G + RT+V + C E + LR G+ +H Y ++G+ MD +G A++
Sbjct: 348 LFVHMRTEGNKEDDRTIVIMFSLCAESADGLRKGKSLHAYASKSGM-RMDVNLGNAMLNM 407
Query: 208 YMRFDAVLS-HRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 267
Y + + + S +VFS M +++S+N +I ++A +++ M ++ ++ T+
Sbjct: 408 YAQQNCIDSVQKVFSEMSNVDVISFNTVILALARNKLGSEAWEVFGLMWELDVEPNSYTI 467
Query: 268 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 327
+ ++ AC + CL +G +S+HGF IK G+E+N SLNT+LT+MYINCGDE A
Sbjct: 468 ISILAACKDETCLNIG--------RSLHGFVIKQGIEVNVSLNTALTDMYINCGDEATAR 527
Query: 328 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISELEPNSVTIVNILTSCTQLAH 387
N+F CP RDL+SWN+LI++Y+KN+ A +A L+F+ MISE+EPNSVTI+NIL+SCT LAH
Sbjct: 528 NLFESCPGRDLISWNALIATYVKNNLAHEAFLVFSRMISEVEPNSVTIINILSSCTHLAH 587
Query: 388 LPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMI 447
LP GQC HAY R+E SL + SL NAFITMYARCG +Q AE+IF TL R+I+SWNA+I
Sbjct: 588 LPQGQCFHAYMLRQESSLGHNLSLGNAFITMYARCGSMQSAERIFKTLPRRNIISWNAII 647
Query: 448 TGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGI 507
TGYGMHGRG DA LAF+QML+DG+ PN V+F SVLSACSHSG+ GL+LF SMV DF I
Sbjct: 648 TGYGMHGRGSDAILAFSQMLEDGYYPNEVTFISVLSACSHSGMIEEGLRLFDSMVHDFHI 707
Query: 508 APQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETIFG 567
PQL HYGC+VDLLGR G EA FI SMPI+PDAS+WRALLS+ + K + IF
Sbjct: 708 TPQLAHYGCVVDLLGRAGCLDEARGFIESMPIKPDASVWRALLSAYRDHCYTKEAKAIFE 767
Query: 568 KLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVHHFT 627
K+VEL+P NPGN++L+ N YAAAGLWS+V QIR L+ +GL KPPG SWIV+ +Q+H F
Sbjct: 768 KIVELDPMNPGNYVLVCNAYAAAGLWSDVRQIRTCLKAKGLRKPPGMSWIVVRSQIHSFA 827
Query: 628 ATDVLHPQSERIYENLNSLTS--------PDRYSLFLSDISY-KRSTSCSI 668
A D HP +++IY NLNSL PD + + D Y +R +C +
Sbjct: 828 AGDRSHPMADKIYANLNSLLHSMKEIGYVPDPHWILHVDEEYIQREANCGL 869
BLAST of Cucsa.065570 vs. TrEMBL
Match:
A0A061GS93_THECC (Pentatricopeptide repeat-containing protein OS=Theobroma cacao GN=TCM_040700 PE=4 SV=1)
HSP 1 Score: 459.1 bits (1180), Expect = 9.2e-126
Identity = 258/579 (44.56%), Postives = 363/579 (62.69%), Query Frame = 1
Query: 11 GLSSTPEQTHLVN-VSKDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKA 70
GL P+ TH+ + KDWNS+IK+ T LKNDHAILSTY++MESLG+TP+ A +PLVLKA
Sbjct: 8 GLHFAPKNTHIKDDPQKDWNSLIKNQTNLKNDHAILSTYSRMESLGLTPNRAALPLVLKA 67
Query: 71 CGRLNAIGNGVRIHSCIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSW 130
C +LNA+ G RIH IR +LI DVRVGTA++DFYCKCG + EA KVF EM ERDLVSW
Sbjct: 68 CVKLNAVETGKRIHLSIRNTNLIEDVRVGTAIIDFYCKCGFIEEARKVFDEMVERDLVSW 127
Query: 131 NALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLR 190
NA+ISGY GC ++E V L + M++ G PNSRT+VA+LLAC E+ E+RLG+EIHGYCLR
Sbjct: 128 NAMISGYAGCGEFEEVVFLVMRMQREGFRPNSRTLVAMLLACQEVAEVRLGKEIHGYCLR 187
Query: 191 NGLFDMDAYVGTALVGFYMRFDAVLSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKL 250
NGLFD+D +VGTAL+GFY+ F+ SH VF LM VRN V WNA+I G+ ++G+ KALKL
Sbjct: 188 NGLFDLDPHVGTALIGFYLSFNVRASHTVFDLMAVRNTVCWNAMIKGYFDIGESLKALKL 247
Query: 251 YSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNT 310
+ ML++G++FD+VTML +IQACAE+G L LG Q+HQ+AIK + N L I
Sbjct: 248 FEKMLMDGVEFDSVTMLALIQACAEFGSLELGSQIHQMAIKC----SYSNDLFI----VN 307
Query: 311 SLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISE-LEP 370
+L MY + G ++A +F P+RD+ WNS+IS+Y + +A LF HM +E +
Sbjct: 308 ALLNMYADIGSLKSACKLFDVTPRRDVALWNSMISAYFEYSCNEEATSLFVHMRTEGNKE 367
Query: 371 NSVTIVNILTSCTQLAH-LPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAE 430
+ TIV + + C + A L G+ LHAY ++ + MD +L NA + MYA+ + +
Sbjct: 368 DDRTIVIMFSLCAESADGLRKGKSLHAYASKS--GMRMDVNLGNAMLNMYAQQNCIDSVQ 427
Query: 431 KIFCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSG 490
K+F + ++S+N +I + G +A F M + +PN+ + S+L+AC
Sbjct: 428 KVFSEMSNVDVISFNTVILALARNKLGSEAWEVFGLMWELDVEPNSYTIISILAACKDET 487
Query: 491 LTVTGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRAL 550
G L H V GI ++ + D+ G + A S P D W AL
Sbjct: 488 CLNIGRSL-HGFVIKQGIEVNVSLNTALTDMYINCGDEATARNLFESCP-GRDLISWNAL 547
Query: 551 LSSCQIKSNNKLLET--IFGKLV-ELEPSNPGNFILLSN 584
+++ NN E +F +++ E+EP++ +LS+
Sbjct: 548 IAT--YVKNNLAHEAFLVFSRMISEVEPNSVTIINILSS 572
HSP 2 Score: 213.0 bits (541), Expect = 1.1e-51
Identity = 153/550 (27.82%), Postives = 272/550 (49.45%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHS-CI 87
WN++I + ++ +M+ G P+S T+ +L AC + + G IH C+
Sbjct: 127 WNAMISGYAGCGEFEEVVFLVMRMQREGFRPNSRTLVAMLLACQEVAEVRLGKEIHGYCL 186
Query: 88 RGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAV 147
R D VGTAL+ FY + A + VF M R+ V WNA+I GY +A+
Sbjct: 187 RNGLFDLDPHVGTALIGFYLSFNVRASHT-VFDLMAVRNTVCWNAMIKGYFDIGESLKAL 246
Query: 148 LLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGF 207
LF +M G+ +S T++AL+ AC E L LG +IH ++ + D ++ AL+
Sbjct: 247 KLFEKMLMDGVEFDSVTMLALIQACAEFGSLELGSQIHQMAIKCS-YSNDLFIVNALLNM 306
Query: 208 YMRFDAVLSH-RVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 267
Y ++ S ++F + R++ WN++I+ + +A L+ M EG K D T+
Sbjct: 307 YADIGSLKSACKLFDVTPRRDVALWNSMISAYFEYSCNEEATSLFVHMRTEGNKEDDRTI 366
Query: 268 LVVIQACAEYGC-LRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYI--NCGDER 327
+++ CAE LR G KS+H +A K+G+ ++ +L ++ MY NC D
Sbjct: 367 VIMFSLCAESADGLRKG--------KSLHAYASKSGMRMDVNLGNAMLNMYAQQNCID-- 426
Query: 328 AATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMIS-ELEPNSVTIVNILTSCT 387
+ +F+ D++S+N++I + +N +A +F M ++EPNS TI++IL +C
Sbjct: 427 SVQKVFSEMSNVDVISFNTVILALARNKLGSEAWEVFGLMWELDVEPNSYTIISILAACK 486
Query: 388 QLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSW 447
L +G+ LH + ++ +E++ SL A MY CG A +F + R ++SW
Sbjct: 487 DETCLNIGRSLHGFVIKQ--GIEVNVSLNTALTDMYINCGDEATARNLFESCPGRDLISW 546
Query: 448 NAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMV- 507
NA+I Y + +A L F++M+ + +PN+V+ ++LS+C+H G Q FH+ +
Sbjct: 547 NALIATYVKNNLAHEAFLVFSRMISE-VEPNSVTIINILSSCTHLAHLPQG-QCFHAYML 606
Query: 508 -RDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKL 567
++ + L+ + + R G A ++P S W A+++ +
Sbjct: 607 RQESSLGHNLSLGNAFITMYARCGSMQSAERIFKTLPRRNIIS-WNAIITGYGMHGRGSD 659
Query: 568 LETIFGKLVE 570
F +++E
Sbjct: 667 AILAFSQMLE 659
HSP 3 Score: 578.2 bits (1489), Expect = 1.4e-161
Identity = 309/669 (46.19%), Postives = 427/669 (63.83%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
WN++I + + +S + QM++ G+ P+S T+ +L AC ++ + +G IH
Sbjct: 98 WNALIHGYVRCCCYKEAISLFMQMQNEGLKPNSRTVVALLSACREVSELRSGQEIHGYAL 157
Query: 88 GLDLIN-DVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAV 147
L + D VGTAL+ FY + + F M R+++SWNA+I+GYV Y A+
Sbjct: 158 RNGLFDLDAHVGTALIGFYLRFD-IKTTRLTFDSMVVRNIISWNAIITGYVEIGEYLMAL 217
Query: 148 LLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGF 207
LFV+M G+ + +++ ++ AC + + LG++IH ++N D D ++
Sbjct: 218 KLFVQMLVDGVKFDYVSMLVVIQACAGIGSIELGKQIHEMAIKNSYSD-DLFICGCFE-- 277
Query: 208 YMRFDAVLSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTML 267
LS ++F + R++ WN++I+ G +A L+S M +EGI+ D T++
Sbjct: 278 -------LSRKLFEFVSSRDVALWNSMISACTEYGFYEEAFSLFSKMRMEGIREDERTIV 337
Query: 268 VVIQ----------------ACAEYGCLRLGMQLH--------QLAIKSIHGFAIKNGLE 327
+++ A A +++ L + +++IHGF IK+G+E
Sbjct: 338 IMLSVCEDLADGLRNGKSLHALARKSGMKMDASLANTLLSMYAEFNLRAIHGFVIKHGIE 397
Query: 328 INTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHM 387
+ SLNT+LT+MY+NCGDE AA +F CP RD++SWN+LI+SYIKN+ GKA LLFN M
Sbjct: 398 ADLSLNTALTDMYMNCGDEAAARTLFEGCPSRDVISWNALIASYIKNNEIGKAQLLFNRM 457
Query: 388 ISELEPNSVTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGK 447
ISE+ PNSVTI+NIL+SCTQLA LPLGQCLHAY RR+ S D SLANAFI+MYAR G
Sbjct: 458 ISEVNPNSVTIINILSSCTQLASLPLGQCLHAYANRRQFSFGFDLSLANAFISMYARSGS 517
Query: 448 LQYAEKIFCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSA 507
+Q AEK+F L R+++SWNA+ITGY MHG G DA AF QML+DGF+PN +F +VLSA
Sbjct: 518 MQNAEKMFKILPKRNVISWNALITGYSMHGHGHDAIHAFLQMLEDGFRPNGATFVAVLSA 577
Query: 508 CSHSGLTVTGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDAS 567
C HSGL GLQLFH+MVRDF I+P+L HYGC+VDLLGR G E FI SMP+E DAS
Sbjct: 578 CRHSGLIEMGLQLFHTMVRDFKISPELVHYGCVVDLLGRAGRLDEGREFIESMPVEADAS 637
Query: 568 IWRALLSSCQIKSNNKLLETIFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLR 627
+WRALL++C++ S KL TIF KLVELEP N GN++L+SNIYAAAGLW EV IR LR
Sbjct: 638 VWRALLNACRLHSATKLAGTIFEKLVELEPMNAGNYVLISNIYAAAGLWMEVRLIRTRLR 697
Query: 628 ERGLGKPPGTSWIVIGNQVHHFTATDVLHPQSERIYENLNSLTSPDRYSLFLSDISYKRS 672
E+GL KPPG SWIV+ +QVH F A D H QS+ IY +LNSL+S + S ++ D+
Sbjct: 698 EKGLEKPPGVSWIVVQSQVHCFVAGDTSHLQSDVIYASLNSLSSLIKESGYIPDL----- 747
BLAST of Cucsa.065570 vs. TrEMBL
Match:
M5VUV6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa027147mg PE=4 SV=1)
HSP 1 Score: 392.5 bits (1007), Expect = 1.1e-105
Identity = 210/387 (54.26%), Postives = 268/387 (69.25%), Query Frame = 1
Query: 34 HHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIRGLDLIN 93
HH KLKNDHAILSTYTQMESLG+ PD+ ++PLVLKAC RL+A+ G IHS IR L+
Sbjct: 3 HHAKLKNDHAILSTYTQMESLGVAPDNTSLPLVLKACARLSAVERGKGIHSSIRNTGLMK 62
Query: 94 DVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAVLLFVEMK 153
DVR+GTALVDFYCK GL+ +A +VF EM ERDLV WNALI GYV C CYKEA+ LF++M+
Sbjct: 63 DVRIGTALVDFYCKGGLIDDAVEVFDEMRERDLVLWNALIHGYVRCCCYKEAISLFMQMQ 122
Query: 154 KAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAV 213
GL PNSRTVVALL AC E+ ELR GQEIHGY LRNGLFD+DA+VGTAL+GFY+RFD
Sbjct: 123 NEGLKPNSRTVVALLSACREVSELRSGQEIHGYALRNGLFDLDAHVGTALIGFYLRFDIK 182
Query: 214 LSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTMLVVIQACA 273
+ F M+VRNI+SWNAIITG++ +G+ ALKL+ ML++G+KFD V+MLVVIQACA
Sbjct: 183 TTRLTFDSMVVRNIISWNAIITGYVEIGEYLMALKLFVQMLVDGVKFDYVSMLVVIQACA 242
Query: 274 EYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAATNMFTRCPQ 333
G + LG Q+H++AIK N+ +++I CG + +F
Sbjct: 243 GIGSIELGKQIHEMAIK-----------------NSYSDDLFI-CGCFELSRKLFEFVSS 302
Query: 334 RDLVSWNSLISSYIKNDNAGKALLLFNHMISE-LEPNSVTIVNILTSCTQLAH-LPLGQC 393
RD+ WNS+IS+ + +A LF+ M E + + TIV +L+ C LA L G+
Sbjct: 303 RDVALWNSMISACTEYGFYEEAFSLFSKMRMEGIREDERTIVIMLSVCEDLADGLRNGKS 362
Query: 394 LHAYTTRREVSLEMDASLANAFITMYA 419
LHA R+ ++MDASLAN ++MYA
Sbjct: 363 LHALA--RKSGMKMDASLANTLLSMYA 369
HSP 2 Score: 139.0 bits (349), Expect = 2.1e-29
Identity = 94/363 (25.90%), Postives = 184/363 (50.69%), Query Frame = 1
Query: 149 FVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGFYM 208
+ +M+ G+ P++ ++ +L AC + + G+ IH GL D +GTALV FY
Sbjct: 17 YTQMESLGVAPDNTSLPLVLKACARLSAVERGKGIHSSIRNTGLMK-DVRIGTALVDFYC 76
Query: 209 RF----DAVLSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVT 268
+ DAV VF M R++V WNA+I G++ +A+ L+ M EG+K ++ T
Sbjct: 77 KGGLIDDAV---EVFDEMRERDLVLWNALIHGYVRCCCYKEAISLFMQMQNEGLKPNSRT 136
Query: 269 MLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGL-EINTSLNTSLTEMYINCGDERA 328
++ ++ AC E LR G + IHG+A++NGL +++ + T+L Y+ D +
Sbjct: 137 VVALLSACREVSELRSGQE--------IHGYALRNGLFDLDAHVGTALIGFYLRF-DIKT 196
Query: 329 ATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISE-LEPNSVTIVNILTSCTQ 388
F R+++SWN++I+ Y++ AL LF M+ + ++ + V+++ ++ +C
Sbjct: 197 TRLTFDSMVVRNIISWNAIITGYVEIGEYLMALKLFVQMLVDGVKFDYVSMLVVIQACAG 256
Query: 389 LAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWN 448
+ + LG+ +H + ++ N++ CG + + K+F + +R + WN
Sbjct: 257 IGSIELGKQIH------------EMAIKNSYSDDLFICGCFELSRKLFEFVSSRDVALWN 316
Query: 449 AMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRD 506
+MI+ +G +A F++M +G + + + +LS C + + H++ R
Sbjct: 317 SMISACTEYGFYEEAFSLFSKMRMEGIREDERTIVIMLSVCEDLADGLRNGKSLHALARK 354
HSP 3 Score: 129.8 bits (325), Expect = 1.3e-26
Identity = 96/306 (31.37%), Postives = 150/306 (49.02%), Query Frame = 1
Query: 247 LKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTS 306
L Y+ M G+ D ++ +V++ACA + G K IH GL +
Sbjct: 14 LSTYTQMESLGVAPDNTSLPLVLKACARLSAVERG--------KGIHSSIRNTGLMKDVR 73
Query: 307 LNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISE- 366
+ T+L + Y G A +F +RDLV WN+LI Y++ +A+ LF M +E
Sbjct: 74 IGTALVDFYCKGGLIDDAVEVFDEMRERDLVLWNALIHGYVRCCCYKEAISLFMQMQNEG 133
Query: 367 LEPNSVTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQY 426
L+PNS T+V +L++C +++ L GQ +H Y R + ++DA + A I Y R ++
Sbjct: 134 LKPNSRTVVALLSACREVSELRSGQEIHGYALRNGL-FDLDAHVGTALIGFYLRFD-IKT 193
Query: 427 AEKIFCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSH 486
F ++ R+I+SWNA+ITGY G A F QML DG K + VS V+ AC+
Sbjct: 194 TRLTFDSMVVRNIISWNAIITGYVEIGEYLMALKLFVQMLVDGVKFDYVSMLVVIQACAG 253
Query: 487 SGLTVTGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWR 546
G G Q+ H M + L GC L R + F++S D ++W
Sbjct: 254 IGSIELGKQI-HEMAIKNSYSDDLFICGCFE--LSR-----KLFEFVSSR----DVALWN 297
Query: 547 ALLSSC 552
+++S+C
Sbjct: 314 SMISAC 297
HSP 4 Score: 576.6 bits (1485), Expect = 3.9e-161
Identity = 301/647 (46.52%), Postives = 420/647 (64.91%), Query Frame = 1
Query: 12 LSSTPEQTHLVNVSKDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACG 71
+SS +V + WN++I + + L + QM G+ D T+ + ++A
Sbjct: 215 ISSLVFDLMIVKSAVSWNAMITGYFGSGDFVKALELFVQMLKDGVKFDMVTILVSIQASA 274
Query: 72 RLNAIGNGVRIHSCIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNA 131
+ + G++IH L N++ + AL++ Y + G + A ++F + D+ WN+
Sbjct: 275 EIGSSELGMQIHQLAIKLSYGNELFIVNALLNMYAEIGNLELACRLFDTVTVHDVPLWNS 334
Query: 132 LISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLE-LRLGQEIHGYCLRN 191
+I+ Y+ CY+EA LF M+ + RT+ +L E+ + L++G+ +H +
Sbjct: 335 MIAAYIDHGCYEEATSLFTTMRTE-TREDERTIAVILSLSAELTDGLKIGRSLHALAYKR 394
Query: 192 GLFDMDAYVGTALVGFYMRFDAVLSH-RVFSLMLVRNIVSWNAIITGFLNVGDCAKALKL 251
+ M+ +G AL+ Y + V +VF+ M ++V +N +I F KA +L
Sbjct: 395 EM-KMNVSLGNALLSMYADLNCVEDALKVFNEMSNIDVVPYNTLILAFSVSNLSGKAWEL 454
Query: 252 YSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNT 311
+ M + ++ TM+ ++ +C + CL +G +S+HGF IKN +EIN SLNT
Sbjct: 455 FGMMRESEVSPNSHTMISLLASCGDEKCLNIG--------RSVHGFIIKNSIEINLSLNT 514
Query: 312 SLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISELEPN 371
SLTEMYINCGD AA +F CP RDL+SWN++I++ +KND G+A+L FN MISE+EPN
Sbjct: 515 SLTEMYINCGDGAAARYLFDTCPSRDLISWNAIIAALLKNDKTGEAILFFNRMISEVEPN 574
Query: 372 SVTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKI 431
SVTI+NIL++CT LA+LP GQCLHAY TRR + ++ SL NAFITMYARCG ++ AEKI
Sbjct: 575 SVTIINILSTCTNLANLPQGQCLHAYATRRFSAFGLNLSLGNAFITMYARCGSIRNAEKI 634
Query: 432 FCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLT 491
F TL R+++SWN MITGYG HG DA L F ML DGF+PN V+F S LSAC H+G+
Sbjct: 635 FETLAKRNVISWNGMITGYGTHGCAYDAILTFKNMLKDGFQPNGVTFLSALSACRHAGMI 694
Query: 492 VTGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLS 551
GLQLF+SMV+DF + P L+HYGC+VDLLGRGG +EA FINSMPIEPDAS+WRALLS
Sbjct: 695 KEGLQLFNSMVQDFKMTPTLSHYGCVVDLLGRGGSLNEAREFINSMPIEPDASVWRALLS 754
Query: 552 SCQIKSNNKLLETIFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKP 611
+C++ SN ++ IF LVELEP+N GN+ILLSNIYAAAG WSEV QIRKWL+++GL KP
Sbjct: 755 ACRVHSNTEIAAEIFENLVELEPTNAGNYILLSNIYAAAGFWSEVRQIRKWLKDKGLKKP 814
Query: 612 PGTSWIVIGNQVHHFTATDVLHPQSERIYENLNSLTSPDRYSLFLSD 657
PGTSW+++ QVH FTA D H QS+RIY NLNSL R +++D
Sbjct: 815 PGTSWLIVRGQVHSFTAGDTSHLQSDRIYGNLNSLLLLTREYGYVTD 851
BLAST of Cucsa.065570 vs. TrEMBL
Match:
A0A067JE40_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_25923 PE=4 SV=1)
HSP 1 Score: 434.1 bits (1115), Expect = 3.2e-118
Identity = 255/594 (42.93%), Postives = 357/594 (60.10%), Query Frame = 1
Query: 1 MEIAVNLSFHGLSSTPEQTHLVNVSKDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDS 60
M+I ++L L E+T SKDWN+IIK+H KL+NDHAILSTYTQMESLG+ PD+
Sbjct: 2 MDIRLSLESLKLPPFRERTQNRTGSKDWNAIIKYHAKLRNDHAILSTYTQMESLGLQPDN 61
Query: 61 ATMPLVLKACGRLNAIGNGVRIHSCIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVE 120
T+PL+ KAC RLNA G +IHS I DLI +VRVGT++VDFYCKCG + EA KVF +
Sbjct: 62 MTLPLIFKACTRLNAFERGKKIHSSIESTDLIKNVRVGTSVVDFYCKCGHILEAHKVFDK 121
Query: 121 MPERDLVSWNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLG 180
M ERDLV WNA+ISGYVGC Y EA+ F M++ GL PNSRT+VALLLAC +LELRLG
Sbjct: 122 MSERDLVLWNAIISGYVGCAYYVEAIGQFRRMQREGLEPNSRTLVALLLACEGILELRLG 181
Query: 181 QEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAVLSHRVFSLMLVRNIVSWNAIITGFLNV 240
QE+HGYCLR+G FD+ ++GTAL+GFY+ FD +S VF LM+V++ VSWNA+ITG+
Sbjct: 182 QELHGYCLRSGYFDLYPHLGTALIGFYLNFDVKISSLVFDLMIVKSAVSWNAMITGYFGS 241
Query: 241 GDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNG 300
GD KAL+L+ ML +G+KFD VT+LV IQA AE G LGMQ+HQLAIK +G +
Sbjct: 242 GDFVKALELFVQMLKDGVKFDMVTILVSIQASAEIGSSELGMQIHQLAIKLSYGNEL--- 301
Query: 301 LEINTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFN 360
+N LN MY G+ A +F D+ WNS+I++YI + +A LF
Sbjct: 302 FIVNALLN-----MYAEIGNLELACRLFDTVTVHDVPLWNSMIAAYIDHGCYEEATSLFT 361
Query: 361 HMISELEPNSVTIVNILTSCTQLAH-LPLGQCLHAYTTRREVSLEMDASLANAFITMYAR 420
M +E + TI IL+ +L L +G+ LHA +RE ++M+ SL NA ++MYA
Sbjct: 362 TMRTETREDERTIAVILSLSAELTDGLKIGRSLHALAYKRE--MKMNVSLGNALLSMYAD 421
Query: 421 CGKLQYAEKIFCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASV 480
++ A K+F + +V +N +I + + A F M + PN+ + S+
Sbjct: 422 LNCVEDALKVFNEMSNIDVVPYNTLILAFSVSNLSGKAWELFGMMRESEVSPNSHTMISL 481
Query: 481 LSACSHSGLTVTGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEP 540
L++C G + H + I L+ + ++ G + A ++ P
Sbjct: 482 LASCGDEKCLNIGRSV-HGFIIKNSIEINLSLNTSLTEMYINCGDGAAARYLFDTCP-SR 541
Query: 541 DASIWRALLSSCQIKSNNKLLETI--FGKLV-ELEPSNPGNFILLSNIYAAAGL 591
D W A++++ + N+K E I F +++ E+EP++ +LS A L
Sbjct: 542 DLISWNAIIAA--LLKNDKTGEAILFFNRMISEVEPNSVTIINILSTCTNLANL 581
HSP 2 Score: 570.9 bits (1470), Expect = 2.2e-159
Identity = 292/634 (46.06%), Postives = 413/634 (65.14%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
WN++I + + L + QM G+ DS T+ +++A ++ G +IH
Sbjct: 223 WNAMIMGYFDVGESSKALRLFEQMLMDGVEFDSVTVLALIQASAEFGSLELGDQIHQMAI 282
Query: 88 GLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAVL 147
ND+ + AL++ Y + G + A K+F +P RD+ WN++IS Y+ + EA+
Sbjct: 283 KCSYSNDLFIVNALINMYAEIGCLKSACKLFDGIPTRDVALWNSMISAYIDYSYHGEAIS 342
Query: 148 LFVEMKKAGLTPNSRTVVALLLACGEMLE-LRLGQEIHGYCLRNGLFDMDAYVGTALVGF 207
LF++M+ G + RT V +L C E + LR G+ +H + + G+ MD +G A++
Sbjct: 343 LFIKMRTEGNKEDERTTVLMLSLCAESADALRKGRSLHAHACKTGM-GMDINIGNAILNM 402
Query: 208 YMRFDAVLSHR-VFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 267
Y + + S R VF M +++S+N +I +A + + M +K ++ T+
Sbjct: 403 YAEQNCMDSVRKVFGQMSNVDVISYNTLILVLARNNLGIEAWETFGIMRESDVKPNSYTI 462
Query: 268 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 327
+ ++ AC + CL +G +S+HGF IK G+E+N L T+LT+MYINCGDE A
Sbjct: 463 ISILAACKDETCLNIG--------RSLHGFVIKQGIEVNAPLKTALTDMYINCGDETTAM 522
Query: 328 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISELEPNSVTIVNILTSCTQLAH 387
+F RDL+SWN+LIS+Y+KN+ A +A L+F+ M+SE+EPNSVTI+NIL+SCT LAH
Sbjct: 523 KLFESSHGRDLISWNALISTYVKNNQAHEAFLVFSRMVSEVEPNSVTIINILSSCTHLAH 582
Query: 388 LPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMI 447
LP G+CLH+Y +RE SL + SL NAFITMYARCG ++ AEKIF TL R+I+SWNA+I
Sbjct: 583 LPQGRCLHSYMIQRESSLGRNLSLQNAFITMYARCGSMRNAEKIFETLTRRNIISWNAII 642
Query: 448 TGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGI 507
TGYGMHGRG DA LA++QML+DGF+PN V+F S+LSACSHSG+ GLQLF SMV DF I
Sbjct: 643 TGYGMHGRGYDAILAYSQMLEDGFQPNEVTFISILSACSHSGMIEEGLQLFDSMVHDFNI 702
Query: 508 APQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETIFG 567
PQL HYGC+VDLLGR G +A FI SMPI+PDASIWR+LLS+ + K + IF
Sbjct: 703 TPQLAHYGCVVDLLGRAGRLDKAREFIESMPIKPDASIWRSLLSAYRDHCYTKDAKAIFE 762
Query: 568 KLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVHHFT 627
K+VEL+P NPGN +LL N+YAAAGLW EV ++R+ LR +GL KPPG SWIV+ +Q+H F
Sbjct: 763 KVVELDPMNPGNHVLLCNVYAAAGLWPEVSEMRRHLRAKGLRKPPGISWIVVRSQIHSFA 822
Query: 628 ATDVLHPQSERIYENLNSLTSPDRYSLFLSDISY 660
A D HP +++IY NLNSL + ++ D+ +
Sbjct: 823 AGDRSHPMADKIYANLNSLLQSIKEIGYVPDLHW 847
BLAST of Cucsa.065570 vs. TAIR10
Match:
AT3G57430.1 (AT3G57430.1 Tetratricopeptide repeat (TPR)-like superfamily protein)
HSP 1 Score: 389.8 bits (1000), Expect = 3.5e-108
Identity = 237/685 (34.60%), Postives = 365/685 (53.28%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRL---NAIGNGVRIHS 87
WNS+I + L + M + P S T+ V+ AC L + G ++H+
Sbjct: 167 WNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHA 226
Query: 88 CIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKE 147
+N + T LV Y K G +A + + RDLV+WN ++S E
Sbjct: 227 YGLRKGELNSFIINT-LVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLE 286
Query: 148 AVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALV 207
A+ EM G+ P+ T+ ++L AC + LR G+E+H Y L+NG D +++VG+ALV
Sbjct: 287 ALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALV 346
Query: 208 GFYMRFDAVLS-HRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIE-GIKFDA 267
Y VLS RVF M R I WNA+I G+ +AL L+ M G+ ++
Sbjct: 347 DMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANS 406
Query: 268 VTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDER 327
TM V+ AC G ++IHGF +K GL+ + + +L +MY G
Sbjct: 407 TTMAGVVPACVRSGAFSRK--------EAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKID 466
Query: 328 AATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMIS------------ELEPNS 387
A +F + RDLV+WN++I+ Y+ +++ ALLL + M + L+PNS
Sbjct: 467 IAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNS 526
Query: 388 VTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIF 447
+T++ IL SC L+ L G+ +HAY + +L D ++ +A + MYA+CG LQ + K+F
Sbjct: 527 ITLMTILPSCAALSALAKGKEIHAYAIKN--NLATDVAVGSALVDMYAKCGCLQMSRKVF 586
Query: 448 CTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTV 507
+ +++++WN +I YGMHG G++A M+ G KPN V+F SV +ACSHSG+
Sbjct: 587 DQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVD 646
Query: 508 TGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIE-PDASIWRALLS 567
GL++F+ M D+G+ P HY C+VDLLGR G EA +N MP + A W +LL
Sbjct: 647 EGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMPRDFNKAGAWSSLLG 706
Query: 568 SCQIKSNNKLLETIFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKP 627
+ +I +N ++ E L++LEP+ +++LL+NIY++AGLW + ++R+ ++E+G+ K
Sbjct: 707 ASRIHNNLEIGEIAAQNLIQLEPNVASHYVLLANIYSSAGLWDKATEVRRNMKEQGVRKE 766
Query: 628 PGTSWIVIGNQVHHFTATDVLHPQSERIYENLNSLTSPDRYSLFLSDIS----YKRSTSC 687
PG SWI G++VH F A D HPQSE++ L +L R ++ D S
Sbjct: 767 PGCSWIEHGDEVHKFVAGDSSHPQSEKLSGYLETLWERMRKEGYVPDTSCVLHNVEEDEK 826
HSP 2 Score: 218.4 bits (555), Expect = 1.4e-56
Identity = 153/590 (25.93%), Postives = 285/590 (48.31%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
W +++ + + TY M LGI PD+ P +LKA L + G +IH+ +
Sbjct: 65 WIDLLRSKVRSNLLREAVLTYVDMIVLGIKPDNYAFPALLKAVADLQDMELGKQIHAHVY 124
Query: 88 GLDL-INDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAV 147
++ V V LV+ Y KCG KVF + ER+ VSWN+LIS ++ A+
Sbjct: 125 KFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMAL 184
Query: 148 LLFVEMKKAGLTPNSRTVVALLLACGEM---LELRLGQEIHGYCLRNGLFDMDAYVGTAL 207
F M + P+S T+V+++ AC + L +G+++H Y LR G ++++++ L
Sbjct: 185 EAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKG--ELNSFIINTL 244
Query: 208 VGFYMRFDAVLSHRV-FSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDA 267
V Y + + S +V R++V+WN +++ +AL+ M++EG++ D
Sbjct: 245 VAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDE 304
Query: 268 VTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNG-LEINTSLNTSLTEMYINCGDE 327
T+ V+ AC+ LR G K +H +A+KNG L+ N+ + ++L +MY NC
Sbjct: 305 FTISSVLPACSHLEMLRTG--------KELHAYALKNGSLDENSFVGSALVDMYCNCKQV 364
Query: 328 RAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHM--ISELEPNSVTIVNILTS 387
+ +F R + WN++I+ Y +N++ +ALLLF M + L NS T+ ++ +
Sbjct: 365 LSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPA 424
Query: 388 CTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIV 447
C + + +H + +R L+ D + N + MY+R GK+ A +IF ++ R +V
Sbjct: 425 CVRSGAFSRKEAIHGFVVKR--GLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLV 484
Query: 448 SWNAMITGYGMHGRGRDATLAFAQMLD-----------DGFKPNNVSFASVLSACSHSGL 507
+WN MITGY DA L +M + KPN+++ ++L +C+
Sbjct: 485 TWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSA 544
Query: 508 TVTGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALL 567
G ++ H+ +A + +VD+ + G + + +P + + W ++
Sbjct: 545 LAKGKEI-HAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIP-QKNVITWNVII 604
Query: 568 SSCQIKSN-NKLLETIFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQI 598
+ + N + ++ + +V+ N FI + + +G+ E ++I
Sbjct: 605 MAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRI 640
BLAST of Cucsa.065570 vs. TAIR10
Match:
AT1G11290.1 (AT1G11290.1 Pentatricopeptide repeat (PPR) superfamily protein)
HSP 1 Score: 386.0 bits (990), Expect = 5.0e-107
Identity = 221/619 (35.70%), Postives = 342/619 (55.25%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
+++++K K+ + L + +M + P +LK CG + G IH +
Sbjct: 103 YHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLV 162
Query: 88 GLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAVL 147
D+ T L + Y KC V EA KVF MPERDLVSWN +++GY + A+
Sbjct: 163 KSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALE 222
Query: 148 LFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGFY 207
+ M + L P+ T+V++L A + + +G+EIHGY +R+G FD + TALV Y
Sbjct: 223 MVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSG-FDSLVNISTALVDMY 282
Query: 208 MRFDAVLSHR-VFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTML 267
+ ++ + R +F ML RN+VSWN++I ++ + +A+ ++ ML EG+K V+++
Sbjct: 283 AKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVM 342
Query: 268 VVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAATN 327
+ ACA+ G L G +H+L+++ GL+ N S+ SL MY C + A +
Sbjct: 343 GALHACADLGDLERGRFIHKLSVEL--------GLDRNVSVVNSLISMYCKCKEVDTAAS 402
Query: 328 MFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISE-LEPNSVTIVNILTSCTQLAH 387
MF + R LVSWN++I + +N AL F+ M S ++P++ T V+++T+ +L+
Sbjct: 403 MFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSI 462
Query: 388 LPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMI 447
+ +H R L+ + + A + MYA+CG + A IF + R + +WNAMI
Sbjct: 463 THHAKWIHGVVMRS--CLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMI 522
Query: 448 TGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGI 507
GYG HG G+ A F +M KPN V+F SV+SACSHSGL GL+ F+ M ++ I
Sbjct: 523 DGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKENYSI 582
Query: 508 APQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETIFG 567
+ HYG MVDLLGR G +EA FI MP++P +++ A+L +CQI N E
Sbjct: 583 ELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEKAAE 642
Query: 568 KLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVHHFT 627
+L EL P + G +LL+NIY AA +W +V Q+R + +GL K PG S + I N+VH F
Sbjct: 643 RLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGCSMVEIKNEVHSFF 702
Query: 628 ATDVLHPQSERIYENLNSL 645
+ HP S++IY L L
Sbjct: 703 SGSTAHPDSKKIYAFLEKL 710
HSP 2 Score: 173.3 bits (438), Expect = 5.1e-43
Identity = 120/438 (27.40%), Postives = 212/438 (48.40%), Query Frame = 1
Query: 167 LLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAV-LSHRVFSLMLVR 226
LL C + ELR +I +NGL+ + + T LV + R+ +V + RVF + +
Sbjct: 43 LLERCSSLKELR---QILPLVFKNGLYQ-EHFFQTKLVSLFCRYGSVDEAARVFEPIDSK 102
Query: 227 NIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLH 286
V ++ ++ GF V D KAL+ + M + ++ +++ C + LR+G
Sbjct: 103 LNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVG---- 162
Query: 287 QLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISS 346
K IHG +K+G ++ T L MY C A +F R P+RDLVSWN++++
Sbjct: 163 ----KEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAG 222
Query: 347 YIKNDNAGKALLLFNHMISE-LEPNSVTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLE 406
Y +N A AL + M E L+P+ +TIV++L + + L + +G+ +H Y R +
Sbjct: 223 YSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRS--GFD 282
Query: 407 MDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQM 466
+++ A + MYA+CG L+ A ++F + R++VSWN+MI Y + ++A L F +M
Sbjct: 283 SLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKM 342
Query: 467 LDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGH 526
LD+G KP +VS L AC+ G G + H + + G+ ++ ++ + +
Sbjct: 343 LDEGVKPTDVSVMGALHACADLGDLERG-RFIHKLSVELGLDRNVSVVNSLISMYCKCKE 402
Query: 527 FSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETI-FGKLVELEPSNPGNFILLSN 586
A + + S W A++ N + ++ + + + P F +S
Sbjct: 403 VDTAASMFGKLQSRTLVS-WNAMILG--FAQNGRPIDALNYFSQMRSRTVKPDTFTYVSV 460
Query: 587 IYAAAGLWSEVVQIRKWL 602
I A A L + KW+
Sbjct: 463 ITAIAEL--SITHHAKWI 460
BLAST of Cucsa.065570 vs. TAIR10
Match:
AT4G18750.1 (AT4G18750.1 Pentatricopeptide repeat (PPR) superfamily protein)
HSP 1 Score: 385.2 bits (988), Expect = 8.5e-107
Identity = 217/612 (35.46%), Postives = 334/612 (54.58%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
WN ++ K + + + +M S G+ DS T V K+ L ++ G ++H I
Sbjct: 163 WNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFIL 222
Query: 88 GLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAVL 147
VG +LV FY K V A KVF EM ERD++SWN++I+GYV ++ +
Sbjct: 223 KSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLS 282
Query: 148 LFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGFY 207
+FV+M +G+ + T+V++ C + + LG+ +H ++ D + T L +
Sbjct: 283 VFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYS 342
Query: 208 MRFDAVLSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTMLV 267
D + VF M R++VS+ ++I G+ G +A+KL+ M EGI D T+
Sbjct: 343 KCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTA 402
Query: 268 VIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAATNM 327
V+ CA Y L G K +H + +N L + ++ +L +MY CG + A +
Sbjct: 403 VLNCCARYRLLDEG--------KRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELV 462
Query: 328 FTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISE--LEPNSVTIVNILTSCTQLAH 387
F+ +D++SWN++I Y KN A +AL LFN ++ E P+ T+ +L +C L+
Sbjct: 463 FSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSA 522
Query: 388 LPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMI 447
G+ +H Y R D +AN+ + MYA+CG L A +F + ++ +VSW MI
Sbjct: 523 FDKGREIHGYIMRN--GYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMI 582
Query: 448 TGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGI 507
GYGMHG G++A F QM G + + +SF S+L ACSHSGL G + F+ M + I
Sbjct: 583 AGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKI 642
Query: 508 APQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETIFG 567
P + HY C+VD+L R G +A FI +MPI PDA+IW ALL C+I + KL E +
Sbjct: 643 EPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPDATIWGALLCGCRIHHDVKLAEKVAE 702
Query: 568 KLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVHHFT 627
K+ ELEP N G ++L++NIYA A W +V ++RK + +RGL K PG SWI I +V+ F
Sbjct: 703 KVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIEIKGRVNIFV 762
Query: 628 ATDVLHPQSERI 638
A D +P++E I
Sbjct: 763 AGDSSNPETENI 764
HSP 2 Score: 216.5 bits (550), Expect = 5.2e-56
Identity = 133/447 (29.75%), Postives = 235/447 (52.57%), Query Frame = 1
Query: 59 DSATMPLVLKACGRLNAIGNGVRIHSCIRGLDLINDVRVGTALVDFYCKCGLVAEASKVF 118
D T+ VL+ C ++ +G + + IRG + D +G+ L Y CG + EAS+VF
Sbjct: 93 DPRTLCSVLQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVF 152
Query: 119 VEMPERDLVSWNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELR 178
E+ + WN L++ + ++ LF +M +G+ +S T + + + +
Sbjct: 153 DEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVH 212
Query: 179 LGQEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAVLSHR-VFSLMLVRNIVSWNAIITGF 238
G+++HG+ L++G + ++ VG +LV FY++ V S R VF M R+++SWN+II G+
Sbjct: 213 GGEQLHGFILKSGFGERNS-VGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGY 272
Query: 239 LNVGDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAI 298
++ G K L ++ ML+ GI+ D T++ V CA+ + LG +H + +K+
Sbjct: 273 VSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFS--- 332
Query: 299 KNGLEINTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALL 358
+ NT L +MY CGD +A +F R +VS+ S+I+ Y + AG+A+
Sbjct: 333 REDRFCNT-----LLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVK 392
Query: 359 LFNHMISE-LEPNSVTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITM 418
LF M E + P+ T+ +L C + L G+ +H + +E L D ++NA + M
Sbjct: 393 LFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWI--KENDLGFDIFVSNALMDM 452
Query: 419 YARCGKLQYAEKIFCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDD-GFKPNNVS 478
YA+CG +Q AE +F ++ + I+SWN +I GY + +A F +L++ F P+ +
Sbjct: 453 YAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERT 512
Query: 479 FASVLSACSHSGLTVTGLQLFHSMVRD 503
A VL AC+ G ++ ++R+
Sbjct: 513 VACVLPACASLSAFDKGREIHGYIMRN 528
HSP 3 Score: 162.9 bits (411), Expect = 6.9e-40
Identity = 114/450 (25.33%), Postives = 210/450 (46.67%), Query Frame = 1
Query: 143 KEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTA 202
+ AV L K + P RT+ ++L C + L+ G+E+ + NG F +D+ +G+
Sbjct: 78 ENAVKLLCVSGKWDIDP--RTLCSVLQLCADSKSLKDGKEVDNFIRGNG-FVIDSNLGSK 137
Query: 203 LVGFYMRF-DAVLSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFD 262
L Y D + RVF + + + WN ++ GD + ++ L+ M+ G++ D
Sbjct: 138 LSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMD 197
Query: 263 AVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDE 322
+ T V ++ + + G QLH GF +K+G S+ SL Y+
Sbjct: 198 SYTFSCVSKSFSSLRSVHGGEQLH--------GFILKSGFGERNSVGNSLVAFYLKNQRV 257
Query: 323 RAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHM-ISELEPNSVTIVNILTSC 382
+A +F +RD++SWNS+I+ Y+ N A K L +F M +S +E + TIV++ C
Sbjct: 258 DSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGC 317
Query: 383 TQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVS 442
+ LG+ +H+ + S E N + MY++CG L A+ +F + RS+VS
Sbjct: 318 ADSRLISLGRAVHSIGVKACFSRE--DRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVS 377
Query: 443 WNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMV 502
+ +MI GY G +A F +M ++G P+ + +VL+ C+ L G ++ H +
Sbjct: 378 YTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRV-HEWI 437
Query: 503 RDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLL 562
++ + + ++D+ + G EA + M ++ D W ++
Sbjct: 438 KENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVK-DIISWNTIIGGYSKNCYANEA 497
Query: 563 ETIFGKLVELEPSNPGNFILLSNIYAAAGL 591
++F L+E + +P + + A A L
Sbjct: 498 LSLFNLLLEEKRFSPDERTVACVLPACASL 512
BLAST of Cucsa.065570 vs. TAIR10
Match:
AT5G27110.1 (AT5G27110.1 Tetratricopeptide repeat (TPR)-like superfamily protein)
HSP 1 Score: 380.9 bits (977), Expect = 1.6e-105
Identity = 219/622 (35.21%), Postives = 349/622 (56.11%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGI-TPDSATMPLVLKACGRLNAIGNGVRIHSCI 87
WNS++ ++K H L + ++ + I PDS T P V+KA G L G IH+ +
Sbjct: 74 WNSLMSGYSKNSMFHDTLEVFKRLLNCSICVPDSFTFPNVIKAYGALGREFLGRMIHTLV 133
Query: 88 RGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAV 147
+ DV V ++LV Y K L + +VF EMPERD+ SWN +IS + ++A+
Sbjct: 134 VKSGYVCDVVVASSLVGMYAKFNLFENSLQVFDEMPERDVASWNTVISCFYQSGEAEKAL 193
Query: 148 LLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGF 207
LF M+ +G PNS ++ + AC +L L G+EIH C++ G F++D YV +ALV
Sbjct: 194 ELFGRMESSGFEPNSVSLTVAISACSRLLWLERGKEIHRKCVKKG-FELDEYVNSALVDM 253
Query: 208 YMRFDAV-LSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 267
Y + D + ++ VF M +++V+WN++I G++ GD +++ + M+IEG + T+
Sbjct: 254 YGKCDCLEVAREVFQKMPRKSLVAWNSMIKGYVAKGDSKSCVEILNRMIIEGTRPSQTTL 313
Query: 268 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 327
++ AC+ L G K IHG+ I++ + + +N SL ++Y CG+ A
Sbjct: 314 TSILMACSRSRNLLHG--------KFIHGYVIRSVVNADIYVNCSLIDLYFKCGEANLAE 373
Query: 328 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISE-LEPNSVTIVNILTSCTQLA 387
+F++ + SWN +ISSYI N KA+ +++ M+S ++P+ VT ++L +C+QLA
Sbjct: 374 TVFSKTQKDVAESWNVMISSYISVGNWFKAVEVYDQMVSVGVKPDVVTFTSVLPACSQLA 433
Query: 388 HLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAM 447
L G+ +H + E LE D L +A + MY++CG + A +IF ++ + +VSW M
Sbjct: 434 ALEKGKQIHLSIS--ESRLETDELLLSALLDMYSKCGNEKEAFRIFNSIPKKDVVSWTVM 493
Query: 448 ITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFG 507
I+ YG HG+ R+A F +M G KP+ V+ +VLSAC H+GL GL+ F M +G
Sbjct: 494 ISAYGSHGQPREALYQFDEMQKFGLKPDGVTLLAVLSACGHAGLIDEGLKFFSQMRSKYG 553
Query: 508 IAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPD-ASIWRALLSSCQIKSNNKLLETI 567
I P + HY CM+D+LGR G EA I P D A + L S+C + + L + I
Sbjct: 554 IEPIIEHYSCMIDILGRAGRLLEAYEIIQQTPETSDNAELLSTLFSACCLHLEHSLGDRI 613
Query: 568 FGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVHH 627
LVE P + +++L N+YA+ W ++R ++E GL K PG SWI + ++V H
Sbjct: 614 ARLLVENYPDDASTYMVLFNLYASGESWDAARRVRLKMKEMGLRKKPGCSWIEMSDKVCH 673
Query: 628 FTATDVLHPQSERIYENLNSLT 646
F A D H ++E +YE L L+
Sbjct: 674 FFAEDRSHLRAENVYECLALLS 684
HSP 2 Score: 209.5 bits (532), Expect = 6.4e-54
Identity = 140/473 (29.60%), Postives = 236/473 (49.89%), Query Frame = 1
Query: 82 IHSCIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPER-DLVSWNALISGYVGCL 141
+H I L L DV + +L++ Y C A VF R D+ WN+L+SGY
Sbjct: 26 VHQRILTLGLRRDVVLCKSLINVYFTCKDHCSARHVFENFDIRSDVYIWNSLMSGYSKNS 85
Query: 142 CYKEAVLLFVEMKKAGL-TPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYV 201
+ + + +F + + P+S T ++ A G + LG+ IH +++G + D V
Sbjct: 86 MFHDTLEVFKRLLNCSICVPDSFTFPNVIKAYGALGREFLGRMIHTLVVKSG-YVCDVVV 145
Query: 202 GTALVGFYMRFDAVL-SHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGI 261
++LVG Y +F+ S +VF M R++ SWN +I+ F G+ KAL+L+ M G
Sbjct: 146 ASSLVGMYAKFNLFENSLQVFDEMPERDVASWNTVISCFYQSGEAEKALELFGRMESSGF 205
Query: 262 KFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINC 321
+ ++V++ V I AC+ L G K IH +K G E++ +N++L +MY C
Sbjct: 206 EPNSVSLTVAISACSRLLWLERG--------KEIHRKCVKKGFELDEYVNSALVDMYGKC 265
Query: 322 GDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISE-LEPNSVTIVNIL 381
A +F + P++ LV+WNS+I Y+ ++ + + N MI E P+ T+ +IL
Sbjct: 266 DCLEVAREVFQKMPRKSLVAWNSMIKGYVAKGDSKSCVEILNRMIIEGTRPSQTTLTSIL 325
Query: 382 TSCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRS 441
+C++ +L G+ +H Y R V+ ++ + + I +Y +CG+ AE +F Q
Sbjct: 326 MACSRSRNLLHGKFIHGYVIRSVVNADIYVNC--SLIDLYFKCGEANLAETVFSKTQKDV 385
Query: 442 IVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFH 501
SWN MI+ Y G A + QM+ G KP+ V+F SVL ACS G Q+ H
Sbjct: 386 AESWNVMISSYISVGNWFKAVEVYDQMVSVGVKPDVVTFTSVLPACSQLAALEKGKQI-H 445
Query: 502 SMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSS 551
+ + + ++D+ + G+ EA NS+P + D W ++S+
Sbjct: 446 LSISESRLETDELLLSALLDMYSKCGNEKEAFRIFNSIP-KKDVVSWTVMISA 485
BLAST of Cucsa.065570 vs. TAIR10
Match:
AT4G39530.1 (AT4G39530.1 Tetratricopeptide repeat (TPR)-like superfamily protein)
HSP 1 Score: 380.6 bits (976), Expect = 2.1e-105
Identity = 213/622 (34.24%), Postives = 337/622 (54.18%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
W ++I K+ + L + Q+ + PD + VL AC L + G +IH+ I
Sbjct: 217 WTTMISGCVKMGRSYVSLQLFYQLMEDNVVPDGYILSTVLSACSILPFLEGGKQIHAHIL 276
Query: 88 GLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAVL 147
L D + L+D Y KCG V A K+F MP ++++SW L+SGY +KEA+
Sbjct: 277 RYGLEMDASLMNVLIDSYVKCGRVIAAHKLFNGMPNKNIISWTTLLSGYKQNALHKEAME 336
Query: 148 LFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGFY 207
LF M K GL P+ ++L +C + L G ++H Y ++ L + D+YV +L+ Y
Sbjct: 337 LFTSMSKFGLKPDMYACSSILTSCASLHALGFGTQVHAYTIKANLGN-DSYVTNSLIDMY 396
Query: 208 MRFDAVLSHR-VFSLMLVRNIVSWNAIITGFLNVG---DCAKALKLYSSMLIEGIKFDAV 267
+ D + R VF + ++V +NA+I G+ +G + +AL ++ M I+ +
Sbjct: 397 AKCDCLTDARKVFDIFAAADVVLFNAMIEGYSRLGTQWELHEALNIFRDMRFRLIRPSLL 456
Query: 268 TMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERA 327
T + +++A A L L K IHG K GL ++ ++L ++Y NC +
Sbjct: 457 TFVSLLRASASLTSLGLS--------KQIHGLMFKYGLNLDIFAGSALIDVYSNCYCLKD 516
Query: 328 ATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHM-ISELEPNSVTIVNILTSCTQ 387
+ +F +DLV WNS+ + Y++ +AL LF + +S P+ T N++T+
Sbjct: 517 SRLVFDEMKVKDLVIWNSMFAGYVQQSENEEALNLFLELQLSRERPDEFTFANMVTAAGN 576
Query: 388 LAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWN 447
LA + LGQ H +R LE + + NA + MYA+CG + A K F + +R +V WN
Sbjct: 577 LASVQLGQEFHCQLLKR--GLECNPYITNALLDMYAKCGSPEDAHKAFDSAASRDVVCWN 636
Query: 448 AMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRD 507
++I+ Y HG G+ A +M+ +G +PN ++F VLSACSH+GL GL+ F M+R
Sbjct: 637 SVISSYANHGEGKKALQMLEKMMSEGIEPNYITFVGVLSACSHAGLVEDGLKQFELMLR- 696
Query: 508 FGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLET 567
FGI P+ HY CMV LLGR G ++A I MP +P A +WR+LLS C N +L E
Sbjct: 697 FGIEPETEHYVCMVSLLGRAGRLNKARELIEKMPTKPAAIVWRSLLSGCAKAGNVELAEH 756
Query: 568 IFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVH 627
+ +P + G+F +LSNIYA+ G+W+E ++R+ ++ G+ K PG SWI I +VH
Sbjct: 757 AAEMAILSDPKDSGSFTMLSNIYASKGMWTEAKKVRERMKVEGVVKEPGRSWIGINKEVH 816
Query: 628 HFTATDVLHPQSERIYENLNSL 645
F + D H ++ +IYE L+ L
Sbjct: 817 IFLSKDKSHCKANQIYEVLDDL 826
HSP 2 Score: 198.0 bits (502), Expect = 1.9e-50
Identity = 152/538 (28.25%), Postives = 267/538 (49.63%), Query Frame = 1
Query: 65 LVLKACGRLNAIGNGVRIHSCIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPER 124
L L+A L N V + GL+L D + L++ Y + G + A KVF +MPER
Sbjct: 51 LQLRASDDLLHYQNVVHGQIIVWGLEL--DTYLSNILINLYSRAGGMVYARKVFEKMPER 110
Query: 125 DLVSWNALISGYVGCLCYKEAVLLFVEMKKAGL-TPNSRTVVALLLACGEMLELR---LG 184
+LVSW+ ++S Y+E++++F+E + +PN + + + AC L+ R +
Sbjct: 111 NLVSWSTMVSACNHHGIYEESLVVFLEFWRTRKDSPNEYILSSFIQACSG-LDGRGRWMV 170
Query: 185 QEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAV-LSHRVFSLMLVRNIVSWNAIITGFLN 244
++ + +++G FD D YVGT L+ FY++ + + VF + ++ V+W +I+G +
Sbjct: 171 FQLQSFLVKSG-FDRDVYVGTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMISGCVK 230
Query: 245 VGDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKN 304
+G +L+L+ ++ + + D + V+ AC+ L G K IH ++
Sbjct: 231 MGRSYVSLQLFYQLMEDNVVPDGYILSTVLSACSILPFLEGG--------KQIHAHILRY 290
Query: 305 GLEINTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLF 364
GLE++ SL L + Y+ CG AA +F P ++++SW +L+S Y +N +A+ LF
Sbjct: 291 GLEMDASLMNVLIDSYVKCGRVIAAHKLFNGMPNKNIISWTTLLSGYKQNALHKEAMELF 350
Query: 365 NHMIS-ELEPNSVTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYA 424
M L+P+ +ILTSC L L G +HAYT + +L D+ + N+ I MYA
Sbjct: 351 TSMSKFGLKPDMYACSSILTSCASLHALGFGTQVHAYTIK--ANLGNDSYVTNSLIDMYA 410
Query: 425 RCGKLQYAEKIFCTLQTRSIVSWNAMITGY---GMHGRGRDATLAFAQMLDDGFKPNNVS 484
+C L A K+F +V +NAMI GY G +A F M +P+ ++
Sbjct: 411 KCDCLTDARKVFDIFAAADVVLFNAMIEGYSRLGTQWELHEALNIFRDMRFRLIRPSLLT 470
Query: 485 FASVLSACSHSGLTVTGL-QLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINS 544
F S+L A + LT GL + H ++ +G+ + ++D+ ++ +
Sbjct: 471 FVSLLRA--SASLTSLGLSKQIHGLMFKYGLNLDIFAGSALIDVYSNCYCLKDSRLVFDE 530
Query: 545 MPIEPDASIWRALLSSCQIKSNNKLLETIFGKLVELEPSNPGNFILLSNIYAAAGLWS 593
M ++ D IW ++ + +S N+ +F +L +L P F + + AA L S
Sbjct: 531 MKVK-DLVIWNSMFAGYVQQSENEEALNLFLEL-QLSRERPDEFTFANMVTAAGNLAS 570
HSP 3 Score: 176.4 bits (446), Expect = 6.0e-44
Identity = 139/542 (25.65%), Postives = 258/542 (47.60%), Query Frame = 1
Query: 57 TPDSATMPLVLKACGRLNAIGNGV--RIHSCIRGLDLINDVRVGTALVDFYCKCGLVAEA 116
+P+ + ++AC L+ G + ++ S + DV VGT L+DFY K G + A
Sbjct: 143 SPNEYILSSFIQACSGLDGRGRWMVFQLQSFLVKSGFDRDVYVGTLLIDFYLKDGNIDYA 202
Query: 117 SKVFVEMPERDLVSWNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEM 176
VF +PE+ V+W +ISG V ++ LF ++ + + P+ + +L AC +
Sbjct: 203 RLVFDALPEKSTVTWTTMISGCVKMGRSYVSLQLFYQLMEDNVVPDGYILSTVLSACSIL 262
Query: 177 LELRLGQEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAVL-SHRVFSLMLVRNIVSWNAI 236
L G++IH + LR GL +MDA + L+ Y++ V+ +H++F+ M +NI+SW +
Sbjct: 263 PFLEGGKQIHAHILRYGL-EMDASLMNVLIDSYVKCGRVIAAHKLFNGMPNKNIISWTTL 322
Query: 237 ITGFLNVGDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIH 296
++G+ +A++L++SM G+K D ++ +CA L G Q +H
Sbjct: 323 LSGYKQNALHKEAMELFTSMSKFGLKPDMYACSSILTSCASLHALGFGTQ--------VH 382
Query: 297 GFAIKNGLEINTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIK---ND 356
+ IK L ++ + SL +MY C A +F D+V +N++I Y +
Sbjct: 383 AYTIKANLGNDSYVTNSLIDMYAKCDCLTDARKVFDIFAAADVVLFNAMIEGYSRLGTQW 442
Query: 357 NAGKALLLFNHMISEL-EPNSVTIVNILTSCTQLAHLPLGQCLHAYTTRREVSLEMDASL 416
+AL +F M L P+ +T V++L + L L L + +H + L +D
Sbjct: 443 ELHEALNIFRDMRFRLIRPSLLTFVSLLRASASLTSLGLSKQIHGLMFK--YGLNLDIFA 502
Query: 417 ANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDDGF 476
+A I +Y+ C L+ + +F ++ + +V WN+M GY +A F ++
Sbjct: 503 GSALIDVYSNCYCLKDSRLVFDEMKVKDLVIWNSMFAGYVQQSENEEALNLFLELQLSRE 562
Query: 477 KPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGIA--PQLTHYGCMVDLLGRGGHFSE 536
+P+ +FA++++A + G Q FH + G+ P +T+ ++D+ + G +
Sbjct: 563 RPDEFTFANMVTAAGNLASVQLG-QEFHCQLLKRGLECNPYITN--ALLDMYAKCGSPED 622
Query: 537 AIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETIFGKLVE--LEPSNPGNFILLSNIY 588
A +S D W +++SS K + K++ +EP N+I +
Sbjct: 623 AHKAFDS-AASRDVVCWNSVISSYANHGEGKKALQMLEKMMSEGIEP----NYITFVGVL 665
BLAST of Cucsa.065570 vs. NCBI nr
Match:
gi|449448940|ref|XP_004142223.1| (PREDICTED: putative pentatricopeptide repeat-containing protein At3g01580 [Cucumis sativus])
HSP 1 Score: 840.1 bits (2169), Expect = 2.7e-240
Identity = 430/628 (68.47%), Postives = 500/628 (79.62%), Query Frame = 1
Query: 21 LVNVSKDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGV 80
LV WN+II + + L Y+ M GI D+ TM +V++AC + G+
Sbjct: 223 LVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGM 282
Query: 81 RIHSCIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCL 140
++H +LIND+ + AL++ Y G + + +F +P D WN++IS Y+G
Sbjct: 283 QLHQLAIKFNLINDLFILNALLNMYSDNGSLESSWALFNAVPTSDAALWNSMISSYIGFG 342
Query: 141 CYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRL-GQEIHGYCLRNGLFDMDAYV 200
+ EA+ LF++M+ + + RT+ +L C ++ + + G+ +H + +++G+ ++DAY+
Sbjct: 343 FHAEAIALFIKMRLERIKEDVRTIAIMLSLCNDLNDGSIWGRGLHAHAMKSGI-ELDAYL 402
Query: 201 GTALVGFYMRFDAVLSHR-VFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGI 260
G AL+ Y++ + + + + VF M +++SWN +I+ F AKA +L+ M I
Sbjct: 403 GNALLSMYVKHNQITAAQYVFEKMRGLDVISWNTMISAFAQSMFRAKAFELFLMMCESEI 462
Query: 261 KFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINC 320
KF++ T++ ++ C + L G +SIHGFAIKNGLEINTSLNTSLTEMYINC
Sbjct: 463 KFNSYTIVSLLAFCKDGSDLVFG--------RSIHGFAIKNGLEINTSLNTSLTEMYINC 522
Query: 321 GDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISELEPNSVTIVNILT 380
GDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISELEPNSVTI+NILT
Sbjct: 523 GDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISELEPNSVTIINILT 582
Query: 381 SCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSI 440
SCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSI
Sbjct: 583 SCTQLAHLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSI 642
Query: 441 VSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHS 500
VSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHS
Sbjct: 643 VSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHS 702
Query: 501 MVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNK 560
MVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNK
Sbjct: 703 MVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNK 762
Query: 561 LLETIFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIG 620
LLETIFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIG
Sbjct: 763 LLETIFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIG 822
Query: 621 NQVHHFTATDVLHPQSERIYENLNSLTS 647
NQVHHFTATDVLHPQSERIYENLNSLTS
Sbjct: 823 NQVHHFTATDVLHPQSERIYENLNSLTS 841
BLAST of Cucsa.065570 vs. NCBI nr
Match:
gi|449448940|ref|XP_004142223.1| (PREDICTED: putative pentatricopeptide repeat-containing protein At3g01580 [Cucumis sativus])
HSP 1 Score: 652.1 bits (1681), Expect = 1.1e-183
Identity = 367/578 (63.49%), Postives = 420/578 (72.66%), Query Frame = 1
Query: 1 MEIAVNLSFHGLSSTPEQTHLVNVSKDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDS 60
MEIAVNLSFHGLSSTPEQTHLVNVSKDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDS
Sbjct: 1 MEIAVNLSFHGLSSTPEQTHLVNVSKDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDS 60
Query: 61 ATMPLVLKACGRLNAIGNGVRIHSCIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVE 120
ATMPLVLKACGRLNAIGNGVRIHS IRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVE
Sbjct: 61 ATMPLVLKACGRLNAIGNGVRIHSFIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVE 120
Query: 121 MPERDLVSWNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLG 180
MPERDLVSWNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLG
Sbjct: 121 MPERDLVSWNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLG 180
Query: 181 QEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAVLSHRVFSLMLVRNIVSWNAIITGFLNV 240
QEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAVLSHRVFSLMLVRNIVSWNAIITGFLNV
Sbjct: 181 QEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAVLSHRVFSLMLVRNIVSWNAIITGFLNV 240
Query: 241 GDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNG 300
GDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIK F + N
Sbjct: 241 GDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIK----FNLIND 300
Query: 301 LEINTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFN 360
L I LN +L MY + G ++ +F P D WNS+ISSYI +A+ LF
Sbjct: 301 LFI---LN-ALLNMYSDNGSLESSWALFNAVPTSDAALWNSMISSYIGFGFHAEAIALFI 360
Query: 361 HM-ISELEPNSVTIVNILTSCTQLAHLPL-GQCLHAYTTRREVSLEMDASLANAFITMYA 420
M + ++ + TI +L+ C L + G+ LHA+ + +E+DA L NA ++MY
Sbjct: 361 KMRLERIKEDVRTIAIMLSLCNDLNDGSIWGRGLHAHAMKS--GIELDAYLGNALLSMYV 420
Query: 421 RCGKLQYAEKIFCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFAS 480
+ ++ A+ +F ++ ++SWN MI+ + A F M + K N+ + S
Sbjct: 421 KHNQITAAQYVFEKMRGLDVISWNTMISAFAQSMFRAKAFELFLMMCESEIKFNSYTIVS 480
Query: 481 VLSACSHSGLTVTGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIE 540
+L+ C V G + H G+ + + ++ G A P +
Sbjct: 481 LLAFCKDGSDLVFGRSI-HGFAIKNGLEINTSLNTSLTEMYINCGDERAATNMFTRCP-Q 540
Query: 541 PDASIWRALLSSCQIKSNN--KLLETIFGKLVELEPSN 575
D W +L+SS IK++N K L + ELEP++
Sbjct: 541 RDLVSWNSLISS-YIKNDNAGKALLLFNHMISELEPNS 565
HSP 2 Score: 211.1 bits (536), Expect = 6.2e-51
Identity = 147/554 (26.53%), Postives = 277/554 (50.00%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHS-CI 87
WN++I + + + +M+ G+TP+S T+ +L ACG + + G IH C+
Sbjct: 129 WNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCL 188
Query: 88 RGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAV 147
R D VGTALV FY + V + +VF M R++VSWNA+I+G++ +A+
Sbjct: 189 RNGLFDMDAYVGTALVGFYMRFDAVL-SHRVFSLMLVRNIVSWNAIITGFLNVGDCAKAL 248
Query: 148 LLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGF 207
L+ M G+ ++ T++ ++ AC E LRLG ++H ++ L + D ++ AL+
Sbjct: 249 KLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKFNLIN-DLFILNALLNM 308
Query: 208 YMRFDAVLSH-RVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 267
Y ++ S +F+ + + WN++I+ ++ G A+A+ L+ M +E IK D T+
Sbjct: 309 YSDNGSLESSWALFNAVPTSDAALWNSMISSYIGFGFHAEAIALFIKMRLERIKEDVRTI 368
Query: 268 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 327
+++ C + + + + +H A+K+G+E++ L +L MY+ AA
Sbjct: 369 AIMLSLCND-------LNDGSIWGRGLHAHAMKSGIELDAYLGNALLSMYVKHNQITAAQ 428
Query: 328 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMI-SELEPNSVTIVNILTSCTQLA 387
+F + D++SWN++IS++ ++ KA LF M SE++ NS TIV++L C +
Sbjct: 429 YVFEKMRGLDVISWNTMISAFAQSMFRAKAFELFLMMCESEIKFNSYTIVSLLAFCKDGS 488
Query: 388 HLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAM 447
L G+ +H + + LE++ SL + MY CG + A +F R +VSWN++
Sbjct: 489 DLVFGRSIHGFAIKN--GLEINTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSL 548
Query: 448 ITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMV--RD 507
I+ Y + A L F M+ + +PN+V+ ++L++C+ G Q H+ R+
Sbjct: 549 ISSYIKNDNAGKALLLFNHMISE-LEPNSVTIINILTSCTQLAHLPLG-QCLHAYTTRRE 608
Query: 508 FGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLET 567
+ + + + R G A ++ S W A+++ + +
Sbjct: 609 VSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVS-WNAMITGYGMHGRGRDATL 668
Query: 568 IFGKLVE--LEPSN 575
F ++++ +P+N
Sbjct: 669 AFAQMLDDGFKPNN 668
HSP 3 Score: 800.0 bits (2065), Expect = 3.1e-228
Identity = 410/621 (66.02%), Postives = 487/621 (78.42%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
WN+II + + L ++ M GI D+ TM +V++AC + G+++H
Sbjct: 230 WNAIITGFLNVGDYTKALKLFSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAI 289
Query: 88 GLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAVL 147
+LINDV V AL++ Y G + + +F +P D WN++IS Y+G + EA+
Sbjct: 290 KFNLINDVFVLNALLNMYSDNGSLESSCVLFNAVPTSDAALWNSMISCYIGFGFHAEAIA 349
Query: 148 LFVEMKKAGLTPNSRTVVALLLACGEMLELRL-GQEIHGYCLRNGLFDMDAYVGTALVGF 207
LF++M+ + + RT+V +L C ++ + L G+ +H + +++G+ ++DA++G AL+
Sbjct: 350 LFIKMRLERIKEDVRTIVIMLSLCNDLNDGSLWGRGLHAHAMKSGI-ELDAFLGNALLSM 409
Query: 208 YMRFDAV-LSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 267
Y++ + + + VF +++SWN +I+ AKA +L+ M IKF++ T+
Sbjct: 410 YVKHNQINAAQNVFEKTRGLDVISWNTMISALAQSMFRAKAFELFFMMCESEIKFNSYTI 469
Query: 268 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 327
+ ++ C + L G +SIHGFAIKNGLEINTSLNTSLTEMYINCGDERAA
Sbjct: 470 ISLLALCKDGNDLVFG--------RSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAI 529
Query: 328 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISELEPNSVTIVNILTSCTQLAH 387
+MFTRCPQRDL+SWNSLI SYIKNDNAGKALLLFNHMISELEPNSVTI+NILTSCTQLAH
Sbjct: 530 DMFTRCPQRDLISWNSLILSYIKNDNAGKALLLFNHMISELEPNSVTIINILTSCTQLAH 589
Query: 388 LPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMI 447
LPLGQCLHAY TRRE SLEMDASLANAFITMYARCGK+QYAE+IF TLQTR+IVSWNAMI
Sbjct: 590 LPLGQCLHAYATRREESLEMDASLANAFITMYARCGKMQYAEQIFRTLQTRNIVSWNAMI 649
Query: 448 TGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGI 507
TGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLT TGL LFHSMVRDFG+
Sbjct: 650 TGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTETGLLLFHSMVRDFGL 709
Query: 508 APQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETIFG 567
APQLTHYGCMVDLLGRGGHFSEAIAFIN+MPIEPDASIWRALLSS QIKSN KLLETIFG
Sbjct: 710 APQLTHYGCMVDLLGRGGHFSEAIAFINTMPIEPDASIWRALLSSWQIKSNKKLLETIFG 769
Query: 568 KLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVHHFT 627
KLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVH+FT
Sbjct: 770 KLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVHYFT 829
Query: 628 ATDVLHPQSERIYENLNSLTS 647
ATDVLHPQSE+IYENLNSLTS
Sbjct: 830 ATDVLHPQSEKIYENLNSLTS 841
BLAST of Cucsa.065570 vs. NCBI nr
Match:
gi|659097605|ref|XP_008449715.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g13600-like [Cucumis melo])
HSP 1 Score: 632.9 bits (1631), Expect = 6.6e-178
Identity = 356/578 (61.59%), Postives = 414/578 (71.63%), Query Frame = 1
Query: 1 MEIAVNLSFHGLSSTPEQTHLVNVSKDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDS 60
MEIAVNLS+HGLSSTP+QTHL+NVSKDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDS
Sbjct: 1 MEIAVNLSYHGLSSTPKQTHLINVSKDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDS 60
Query: 61 ATMPLVLKACGRLNAIGNGVRIHSCIRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVE 120
ATMPLVLKACGRLNAI GVRIHSCIRG DLINDVRVGTALVDFYCKCGLVAEASKVFVE
Sbjct: 61 ATMPLVLKACGRLNAIDKGVRIHSCIRGSDLINDVRVGTALVDFYCKCGLVAEASKVFVE 120
Query: 121 MPERDLVSWNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLG 180
MPERDLVSWNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLG
Sbjct: 121 MPERDLVSWNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLG 180
Query: 181 QEIHGYCLRNGLFDMDAYVGTALVGFYMRFDAVLSHRVFSLMLVRNIVSWNAIITGFLNV 240
QEIHGYCLRNGLFDMDAYVGTALVGFY+RFDAVLSHRVFSLM+VRNIVSWNAIITGFLNV
Sbjct: 181 QEIHGYCLRNGLFDMDAYVGTALVGFYLRFDAVLSHRVFSLMVVRNIVSWNAIITGFLNV 240
Query: 241 GDCAKALKLYSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNG 300
GD KALKL+SSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIK F + N
Sbjct: 241 GDYTKALKLFSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIK----FNLIND 300
Query: 301 LEINTSLNTSLTEMYINCGDERAATNMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFN 360
+ + LN +L MY + G ++ +F P D WNS+IS YI +A+ LF
Sbjct: 301 VFV---LN-ALLNMYSDNGSLESSCVLFNAVPTSDAALWNSMISCYIGFGFHAEAIALFI 360
Query: 361 HM-ISELEPNSVTIVNILTSCTQLAHLPL-GQCLHAYTTRREVSLEMDASLANAFITMYA 420
M + ++ + TIV +L+ C L L G+ LHA+ + +E+DA L NA ++MY
Sbjct: 361 KMRLERIKEDVRTIVIMLSLCNDLNDGSLWGRGLHAHAMKS--GIELDAFLGNALLSMYV 420
Query: 421 RCGKLQYAEKIFCTLQTRSIVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFAS 480
+ ++ A+ +F + ++SWN MI+ A F M + K N+ + S
Sbjct: 421 KHNQINAAQNVFEKTRGLDVISWNTMISALAQSMFRAKAFELFFMMCESEIKFNSYTIIS 480
Query: 481 VLSACSHSGLTVTGLQLFHSMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIE 540
+L+ C V G + H G+ + + ++ G AI P +
Sbjct: 481 LLALCKDGNDLVFGRSI-HGFAIKNGLEINTSLNTSLTEMYINCGDERAAIDMFTRCP-Q 540
Query: 541 PDASIWRALLSSCQIKSNN--KLLETIFGKLVELEPSN 575
D W +L+ S IK++N K L + ELEP++
Sbjct: 541 RDLISWNSLILS-YIKNDNAGKALLLFNHMISELEPNS 565
HSP 2 Score: 215.7 bits (548), Expect = 2.5e-52
Identity = 151/554 (27.26%), Postives = 277/554 (50.00%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHS-CI 87
WN++I + + + +M+ G+TP+S T+ +L ACG + + G IH C+
Sbjct: 129 WNALISGYVGCLCYKEAVLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCL 188
Query: 88 RGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAV 147
R D VGTALV FY + V + +VF M R++VSWNA+I+G++ Y +A+
Sbjct: 189 RNGLFDMDAYVGTALVGFYLRFDAVL-SHRVFSLMVVRNIVSWNAIITGFLNVGDYTKAL 248
Query: 148 LLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGF 207
LF M G+ ++ T++ ++ AC E LRLG ++H ++ L + D +V AL+
Sbjct: 249 KLFSSMLIEGIKFDAVTMLVVIQACAEYGCLRLGMQLHQLAIKFNLIN-DVFVLNALLNM 308
Query: 208 YMRFDAVLSHRV-FSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 267
Y ++ S V F+ + + WN++I+ ++ G A+A+ L+ M +E IK D T+
Sbjct: 309 YSDNGSLESSCVLFNAVPTSDAALWNSMISCYIGFGFHAEAIALFIKMRLERIKEDVRTI 368
Query: 268 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 327
++++ C + + L + +H A+K+G+E++ L +L MY+ AA
Sbjct: 369 VIMLSLCND-------LNDGSLWGRGLHAHAMKSGIELDAFLGNALLSMYVKHNQINAAQ 428
Query: 328 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMI-SELEPNSVTIVNILTSCTQLA 387
N+F + D++SWN++IS+ ++ KA LF M SE++ NS TI+++L C
Sbjct: 429 NVFEKTRGLDVISWNTMISALAQSMFRAKAFELFFMMCESEIKFNSYTIISLLALCKDGN 488
Query: 388 HLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAM 447
L G+ +H + + LE++ SL + MY CG + A +F R ++SWN++
Sbjct: 489 DLVFGRSIHGFAIKN--GLEINTSLNTSLTEMYINCGDERAAIDMFTRCPQRDLISWNSL 548
Query: 448 ITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMV--RD 507
I Y + A L F M+ + +PN+V+ ++L++C+ G Q H+ R+
Sbjct: 549 ILSYIKNDNAGKALLLFNHMISE-LEPNSVTIINILTSCTQLAHLPLG-QCLHAYATRRE 608
Query: 508 FGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLET 567
+ + + + R G A ++ S W A+++ + +
Sbjct: 609 ESLEMDASLANAFITMYARCGKMQYAEQIFRTLQTRNIVS-WNAMITGYGMHGRGRDATL 668
Query: 568 IFGKLVE--LEPSN 575
F ++++ +P+N
Sbjct: 669 AFAQMLDDGFKPNN 668
HSP 3 Score: 679.5 bits (1752), Expect = 6.2e-192
Identity = 350/642 (54.52%), Postives = 450/642 (70.09%), Query Frame = 1
Query: 26 KDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSC 85
KDWN++I+ H KL+ND AILSTY +M S ++PD A +PLVLKAC RL+ + G RIHS
Sbjct: 26 KDWNALIRRHAKLRNDGAILSTYARMLSSRVSPDEAALPLVLKACSRLDDVELGKRIHSS 85
Query: 86 IRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEA 145
I G DL+ DVRV T+LVDFY KCGLV +A VF M ERD+V WNA+I+GYVG ++EA
Sbjct: 86 ILGTDLMEDVRVATSLVDFYRKCGLVEDARYVFDRMAERDVVMWNAMINGYVGVGDHEEA 145
Query: 146 VLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVG 205
+ +FVEM + + P+SRTVVALLLACGE +ELR+GQEIH Y LR+GLF +VGTAL+G
Sbjct: 146 ISVFVEMGRRSVRPSSRTVVALLLACGEAMELRMGQEIHCYSLRSGLFHECPHVGTALIG 205
Query: 206 FYMRFDAVLSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 265
FY +F+ + H VF +M V+N+VSWNAIITG+ G+ L+L+ +L EG K D VTM
Sbjct: 206 FYAKFNLKVLHSVFDMMEVKNMVSWNAIITGYFEAGEYLMILELFVRILEEGGKLDEVTM 265
Query: 266 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 325
L+VIQAC EYG L L MQ+HQ+A+KS L + +L MY A
Sbjct: 266 LIVIQACTEYGSLGLAMQIHQIALKS--------DLSRDVYSANALLHMYSKLQKPEFAY 325
Query: 326 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMI---SELEPNSVTIVNILTSCTQ 385
+F P RD+ WNS++S+Y +A LF M+ SE++PNS TI++IL S
Sbjct: 326 EIFKSVPTRDVALWNSMLSTYNDFSCPEEAQRLFLRMLTEDSEIKPNSHTIISILASYDD 385
Query: 386 LAHLPLGQ-----CLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRS 445
L +G+ C+HAY TRRE S+ DASLANA ITMYA+CG L AEK+F + R
Sbjct: 386 APSLNIGRSIHGYCIHAYITRRESSIGSDASLANALITMYAKCGSLHNAEKVFNLIWRRD 445
Query: 446 IVSWNAMITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFH 505
++SWNA+ITGYG+HG+G A F +ML+DG KPN+ +F S+LSACSHSGL GL LF
Sbjct: 446 VISWNALITGYGIHGQGEGAIPTFLKMLEDGMKPNSATFVSILSACSHSGLIEEGLLLFS 505
Query: 506 SMVRDFGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNN 565
SM F + P+L HYGC+VDLLGRGG+ EA+ FINSMPIE DAS+WRALLS+CQ+ SN
Sbjct: 506 SMFCKFKVNPELIHYGCVVDLLGRGGYLGEALDFINSMPIEADASVWRALLSACQVHSNT 565
Query: 566 KLLETIFGKLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVI 625
KL++ +F K+VELEP N GN++LLSNIYA AGLWSEV IR WL+E+GL KPPG SWIVI
Sbjct: 566 KLVKLVFEKIVELEPMNEGNYVLLSNIYATAGLWSEVGLIRAWLKEKGLRKPPGISWIVI 625
Query: 626 GNQVHHFTATDVLHPQSERIYENLNSLTSPDRYSLFLSDISY 660
+Q H FTA D HP+++ IY L+SL+ + + ++SDI +
Sbjct: 626 RDQAHVFTARDQSHPRTDLIYAILSSLSCSIKDAGYVSDIQW 659
BLAST of Cucsa.065570 vs. NCBI nr
Match:
gi|731392814|ref|XP_010651228.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g33680-like [Vitis vinifera])
HSP 1 Score: 599.0 bits (1543), Expect = 1.1e-167
Identity = 308/634 (48.58%), Postives = 423/634 (66.72%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
WN++I + + + L + QM + D TM + ++AC L ++ G +IH
Sbjct: 230 WNAMISGYYDVGDYFKALELFVQMLVDEVKFDCVTMLVAVQACAELGSLKLGKQIHQLAI 289
Query: 88 GLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAVL 147
+ + D+ + AL++ Y G + + ++F +P RD WN++IS Y C++EA+
Sbjct: 290 KFEFVEDLYILNALLNMYSNNGSLESSHQLFESVPNRDAPLWNSMISAYAAFGCHEEAMD 349
Query: 148 LFVEMKKAGLTPNSRTVVALLLACGEMLE-LRLGQEIHGYCLRNGLFDMDAYVGTALVGF 207
LF+ M+ G+ + RTVV +L C E+ L G+ +H + +++G+ +DA +G AL+
Sbjct: 350 LFIRMQSEGVKKDERTVVIMLSMCEELASGLLKGKSLHAHVIKSGM-RIDASLGNALLSM 409
Query: 208 YMRFDAVLS-HRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 267
Y + V S ++F M +I+SWN +I A+A +L+ M IK ++ T+
Sbjct: 410 YTELNCVESVQKIFDRMKGVDIISWNTMILALARNTLRAQACELFERMRESEIKPNSYTI 469
Query: 268 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 327
+ ++ AC + CL G +SIHG+ +K+ +EIN L T+L +MY+NCGDE A
Sbjct: 470 ISILAACEDVTCLDFG--------RSIHGYVMKHSIEINQPLRTALADMYMNCGDEATAR 529
Query: 328 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISELEPNSVTIVNILTSCTQLAH 387
++F CP RDL+SWN++I+SY+KN+ A KALLLF+ MISE EPNSVTI+N+L+S T LA
Sbjct: 530 DLFEGCPDRDLISWNAMIASYVKNNQAHKALLLFHRMISEAEPNSVTIINVLSSFTHLAT 589
Query: 388 LPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMI 447
LP GQ LHAY TRR SL +D SLANAFITMYARCG LQ AE IF TL R+I+SWNAMI
Sbjct: 590 LPQGQSLHAYVTRRGFSLGLDLSLANAFITMYARCGSLQSAENIFKTLPKRNIISWNAMI 649
Query: 448 TGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGI 507
GYGM+GRG DA LAF+QML+DGF+PN V+F SVLSACSHSG GLQLFHSMV+DF +
Sbjct: 650 AGYGMNGRGSDAMLAFSQMLEDGFRPNGVTFVSVLSACSHSGFIEMGLQLFHSMVQDFNV 709
Query: 508 APQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETIFG 567
P+L HY C+VDLL RGG EA FI+SMPIEPDAS+WRALLSSC+ S+ K +TIF
Sbjct: 710 TPELVHYSCIVDLLARGGCIDEAREFIDSMPIEPDASVWRALLSSCRAYSDAKQAKTIFE 769
Query: 568 KLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVHHFT 627
KL +LEP N GN++LLSN+YA AGLW EV +IR WL+E+GL KPPG SWI++ NQVH F+
Sbjct: 770 KLDKLEPMNAGNYVLLSNVYATAGLWLEVRRIRTWLKEKGLRKPPGISWIIVKNQVHCFS 829
Query: 628 ATDVLHPQSERIYENLNSLTSPDRYSLFLSDISY 660
A D HPQS++IY L+ L S R + + D+ +
Sbjct: 830 AGDRSHPQSDKIYAKLSILLSSMRETGYDPDLRW 854
BLAST of Cucsa.065570 vs. NCBI nr
Match:
gi|731392814|ref|XP_010651228.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g33680-like [Vitis vinifera])
HSP 1 Score: 425.2 bits (1092), Expect = 2.1e-115
Identity = 251/569 (44.11%), Postives = 343/569 (60.28%), Query Frame = 1
Query: 26 KDWNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSC 85
K WNS+IKH LKND AILS YTQMESLG+ P++ T+PLVLKAC NA+ G IH
Sbjct: 26 KHWNSVIKHQANLKNDQAILSAYTQMESLGVLPNNTTLPLVLKACAAQNAVERGKSIHRS 85
Query: 86 IRGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEA 145
I+G DL++DVRVGTA+VDFYCKCG V +A VF M +RD+V WNA++ GYVG CY+EA
Sbjct: 86 IQGTDLMDDVRVGTAVVDFYCKCGFVEDARCVFDAMSDRDVVLWNAMVYGYVGWGCYEEA 145
Query: 146 VLLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVG 205
+LL EM + L PNSRT+VALLLAC ELRLG+ +HGYCLRNG+FD + +V TAL+G
Sbjct: 146 MLLVREMGRENLRPNSRTMVALLLACEGASELRLGRGVHGYCLRNGMFDSNPHVATALIG 205
Query: 206 FYMRFDAVLSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 265
FY+RFD + +F LM+VRNIVSWNA+I+G+ +VGD KAL+L+ ML++ +KFD VTM
Sbjct: 206 FYLRFDMRVLPLLFDLMVVRNIVSWNAMISGYYDVGDYFKALELFVQMLVDEVKFDCVTM 265
Query: 266 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 325
LV +QACAE G L+LG Q+HQLAIK F L I LN +L MY N G ++
Sbjct: 266 LVAVQACAELGSLKLGKQIHQLAIK----FEFVEDLYI---LN-ALLNMYSNNGSLESSH 325
Query: 326 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISE-LEPNSVTIVNILTSCTQLA 385
+F P RD WNS+IS+Y +A+ LF M SE ++ + T+V +L+ C +LA
Sbjct: 326 QLFESVPNRDAPLWNSMISAYAAFGCHEEAMDLFIRMQSEGVKKDERTVVIMLSMCEELA 385
Query: 386 H-LPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNA 445
L G+ LHA+ + + +DASL NA ++MY ++ +KIF ++ I+SWN
Sbjct: 386 SGLLKGKSLHAHVIKS--GMRIDASLGNALLSMYTELNCVESVQKIFDRMKGVDIISWNT 445
Query: 446 MITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDF 505
MI + A F +M + KPN+ + S+L+AC G + H V
Sbjct: 446 MILALARNTLRAQACELFERMRESEIKPNSYTIISILAACEDVTCLDFGRSI-HGYVMKH 505
Query: 506 GIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSN--NKLLE 565
I + D+ G + A P + D W A+++S +K+N +K L
Sbjct: 506 SIEINQPLRTALADMYMNCGDEATARDLFEGCP-DRDLISWNAMIAS-YVKNNQAHKALL 565
Query: 566 TIFGKLVELEPSNPGNFILLSNIYAAAGL 591
+ E EP++ +LS+ A L
Sbjct: 566 LFHRMISEAEPNSVTIINVLSSFTHLATL 581
HSP 2 Score: 219.9 bits (559), Expect = 1.3e-53
Identity = 155/547 (28.34%), Postives = 270/547 (49.36%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHS-CI 87
WN+++ + + +M + P+S TM +L AC + + G +H C+
Sbjct: 129 WNAMVYGYVGWGCYEEAMLLVREMGRENLRPNSRTMVALLLACEGASELRLGRGVHGYCL 188
Query: 88 RGLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAV 147
R ++ V TAL+ FY + + +F M R++VSWNA+ISGY Y +A+
Sbjct: 189 RNGMFDSNPHVATALIGFYLRFDMRV-LPLLFDLMVVRNIVSWNAMISGYYDVGDYFKAL 248
Query: 148 LLFVEMKKAGLTPNSRTVVALLLACGEMLELRLGQEIHGYCLRNGLFDMDAYVGTALVGF 207
LFV+M + + T++ + AC E+ L+LG++IH ++ + D Y+ AL+
Sbjct: 249 ELFVQMLVDEVKFDCVTMLVAVQACAELGSLKLGKQIHQLAIKFEFVE-DLYILNALLNM 308
Query: 208 YMRFDAV-LSHRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 267
Y ++ SH++F + R+ WN++I+ + G +A+ L+ M EG+K D T+
Sbjct: 309 YSNNGSLESSHQLFESVPNRDAPLWNSMISAYAAFGCHEEAMDLFIRMQSEGVKKDERTV 368
Query: 268 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 327
++++ C E L G+ L KS+H IK+G+ I+ SL +L MY +
Sbjct: 369 VIMLSMCEE---LASGL----LKGKSLHAHVIKSGMRIDASLGNALLSMYTELNCVESVQ 428
Query: 328 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMI-SELEPNSVTIVNILTSCTQLA 387
+F R D++SWN++I + +N +A LF M SE++PNS TI++IL +C +
Sbjct: 429 KIFDRMKGVDIISWNTMILALARNTLRAQACELFERMRESEIKPNSYTIISILAACEDVT 488
Query: 388 HLPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAM 447
L G+ +H Y + S+E++ L A MY CG A +F R ++SWNAM
Sbjct: 489 CLDFGRSIHGYVMKH--SIEINQPLRTALADMYMNCGDEATARDLFEGCPDRDLISWNAM 548
Query: 448 ITGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMV--RD 507
I Y + + A L F +M+ + +PN+V+ +VLS+ +H G Q H+ V R
Sbjct: 549 IASYVKNNQAHKALLLFHRMISEA-EPNSVTIINVLSSFTHLATLPQG-QSLHAYVTRRG 608
Query: 508 FGIAPQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLET 567
F + L+ + + R G A ++P + + W A+++ +
Sbjct: 609 FSLGLDLSLANAFITMYARCGSLQSAENIFKTLP-KRNIISWNAMIAGYGMNGRGSDAML 661
Query: 568 IFGKLVE 570
F +++E
Sbjct: 669 AFSQMLE 661
HSP 3 Score: 598.6 bits (1542), Expect = 1.4e-167
Identity = 308/646 (47.68%), Postives = 427/646 (66.10%), Query Frame = 1
Query: 28 WNSIIKHHTKLKNDHAILSTYTQMESLGITPDSATMPLVLKACGRLNAIGNGVRIHSCIR 87
WN+II + ++ L + QM G+ D +M +V++AC + +I G +IH
Sbjct: 224 WNAIITGYVEIGEYLMALKLFVQMLVDGVKSDYVSMLVVIQACAGIGSIELGRQIHQMAI 283
Query: 88 GLDLINDVRVGTALVDFYCKCGLVAEASKVFVEMPERDLVSWNALISGYVGCLCYKEAVL 147
+D+ + AL++ Y +CG + K+F + RD+ WN++IS + Y+EA+
Sbjct: 284 KNSYSDDLFIVNALLNMYSECGCFELSRKLFEFVSSRDVALWNSMISACIEYGFYEEALS 343
Query: 148 LFVEMKKAGLTPNSRTVVALLLACGEMLE-LRLGQEIHGYCLRNGLFDMDAYVGTALVGF 207
LF +M+ G+ + RT+V +L C ++ + LR G+ +H ++G+ MDA +G L+
Sbjct: 344 LFSKMRMEGIREDERTIVIMLSVCEDLADGLRNGKSLHALARKSGM-KMDASLGNTLLSM 403
Query: 208 YMRFDAVLS-HRVFSLMLVRNIVSWNAIITGFLNVGDCAKALKLYSSMLIEGIKFDAVTM 267
Y F+ V S +VF+ M +++SWN +I G +A K++ M K ++ T+
Sbjct: 404 YAEFNCVESVQKVFAEMKCSDVISWNTLIRALACNGLQDEAWKIFGVMRESDTKPNSHTI 463
Query: 268 LVVIQACAEYGCLRLGMQLHQLAIKSIHGFAIKNGLEINTSLNTSLTEMYINCGDERAAT 327
+ ++ C + C+ + +++IHGF IK+G+E + SLNT+LT+MY+NCGDE AA
Sbjct: 464 ISILATCEDETCINI--------VRAIHGFVIKHGIEADLSLNTALTDMYMNCGDEAAAR 523
Query: 328 NMFTRCPQRDLVSWNSLISSYIKNDNAGKALLLFNHMISELEPNSVTIVNILTSCTQLAH 387
+F CP RD++SWN+LI+SYIKN+ GKA LLFN M+SE+ PNSVTI+NIL+SCTQLA
Sbjct: 524 TLFEGCPSRDVISWNALIASYIKNNEIGKAQLLFNRMVSEVNPNSVTIINILSSCTQLAS 583
Query: 388 LPLGQCLHAYTTRREVSLEMDASLANAFITMYARCGKLQYAEKIFCTLQTRSIVSWNAMI 447
LPLGQCLHAY RR+ S D SLANAFI+MYAR G +Q AEKIF L R+++SWNA+I
Sbjct: 584 LPLGQCLHAYANRRQFSFGFDLSLANAFISMYARSGSMQNAEKIFKILPKRNVISWNALI 643
Query: 448 TGYGMHGRGRDATLAFAQMLDDGFKPNNVSFASVLSACSHSGLTVTGLQLFHSMVRDFGI 507
TGY MHG G DA AF QML+DGF+PN +F +VLSAC HSGL GLQLFH+MVRDF I
Sbjct: 644 TGYSMHGHGHDAIHAFLQMLEDGFRPNGATFVAVLSACRHSGLIEMGLQLFHTMVRDFKI 703
Query: 508 APQLTHYGCMVDLLGRGGHFSEAIAFINSMPIEPDASIWRALLSSCQIKSNNKLLETIFG 567
+P+L HYGC+VDLLGR G E FI SMP+E DAS+WRALL++C++ S KL IF
Sbjct: 704 SPELVHYGCVVDLLGRAGRLDEGREFIESMPVEADASVWRALLNACRLHSATKLAGAIFE 763
Query: 568 KLVELEPSNPGNFILLSNIYAAAGLWSEVVQIRKWLRERGLGKPPGTSWIVIGNQVHHFT 627
KLVELEP N GN++L+SNIYAAAGLW EV QIR LRE+GL KPPG SWIV+ +QVH F
Sbjct: 764 KLVELEPMNAGNYVLISNIYAAAGLWMEVRQIRTRLREKGLEKPPGVSWIVVQSQVHCFV 823
Query: 628 ATDVLHPQSERIYENLNSLTSPDRYSLFLSDISYKRSTSCSILLCD 672
A D H QS+ IY +LNSL++ + S ++ D+ ++LCD
Sbjct: 824 AGDTSHLQSDMIYASLNSLSTLIKESGYIPDL--------RLVLCD 852
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| PP285_ARATH | 6.1e-107 | 34.60 | Pentatricopeptide repeat-containing protein At3g57430, chloroplastic OS=Arabidop... | [more] |
| PPR32_ARATH | 8.9e-106 | 35.70 | Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidop... | [more] |
| PP320_ARATH | 1.5e-105 | 35.46 | Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis t... | [more] |
| PP398_ARATH | 2.8e-104 | 35.21 | Pentatricopeptide repeat-containing protein At5g27110 OS=Arabidopsis thaliana GN... | [more] |
| PP357_ARATH | 3.7e-104 | 34.24 | Pentatricopeptide repeat-containing protein At4g39530 OS=Arabidopsis thaliana GN... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A059D8T5_EUCGR | 4.3e-192 | 54.52 | Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B03685 PE=4 SV=1 | [more] |
| A0A061GS93_THECC | 1.5e-165 | 47.16 | Pentatricopeptide repeat-containing protein OS=Theobroma cacao GN=TCM_040700 PE=... | [more] |
| A0A061GS93_THECC | 9.2e-126 | 44.56 | Pentatricopeptide repeat-containing protein OS=Theobroma cacao GN=TCM_040700 PE=... | [more] |
| M5VUV6_PRUPE | 1.1e-105 | 54.26 | Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa027147mg PE=4 SV=1 | [more] |
| A0A067JE40_JATCU | 3.2e-118 | 42.93 | Uncharacterized protein OS=Jatropha curcas GN=JCGZ_25923 PE=4 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G57430.1 | 3.5e-108 | 34.60 | Tetratricopeptide repeat (TPR)-like superfamily protein | [more] |
| AT1G11290.1 | 5.0e-107 | 35.70 | Pentatricopeptide repeat (PPR) superfamily protein | [more] |
| AT4G18750.1 | 8.5e-107 | 35.46 | Pentatricopeptide repeat (PPR) superfamily protein | [more] |
| AT5G27110.1 | 1.6e-105 | 35.21 | Tetratricopeptide repeat (TPR)-like superfamily protein | [more] |
| AT4G39530.1 | 2.1e-105 | 34.24 | Tetratricopeptide repeat (TPR)-like superfamily protein | [more] |
