CsGy5G018950 (gene) Cucumber (Gy14) v2

BLAST of CsGy5G018950 vs. NCBI nr
Match: XP_004150118.1 (PREDICTED: pectinesterase [Cucumis sativus] >KGN51481.1 hypothetical protein Csa_5G568300 [Cucumis sativus])

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60
           MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF
Sbjct: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60

Query: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120
           RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST
Sbjct: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120

Query: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180
           SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK
Sbjct: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240
           AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE
Sbjct: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240

Query: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300
           AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG
Sbjct: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300

Query: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360
           STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA
Sbjct: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360

Query: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420
           HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV
Sbjct: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420

Query: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480
           IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT
Sbjct: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480

Query: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL
Sbjct: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 539

BLAST of CsGy5G018950 vs. NCBI nr
Match: XP_008467117.1 (PREDICTED: pectinesterase [Cucumis melo])

HSP 1 Score: 979.2 bits (2530), Expect = 5.8e-282
Identity = 486/539 (90.17%), Postives = 505/539 (93.69%), Query Frame = 0

Query: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60
           MK ++CFLLFSLLVIIHGRSFNNFMSC QTPYPDLCFHYINPNDNI TA IDETYL TRF
Sbjct: 1   MKFVYCFLLFSLLVIIHGRSFNNFMSCFQTPYPDLCFHYINPNDNIPTADIDETYLNTRF 60

Query: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120
           RNSA+QATL QA KV DHLRS MDLGSFDD++RN VALIDCLEFYEDSIA+LNRS  SST
Sbjct: 61  RNSAVQATLNQARKVHDHLRSIMDLGSFDDDNRNKVALIDCLEFYEDSIAKLNRSVFSST 120

Query: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180
           S NSIDH TMLSASLANHQTCLDGFRDF    D+SNNFFLPIQIMSNFSKLVSNSLAITK
Sbjct: 121 SVNSIDHLTMLSASLANHQTCLDGFRDF----DNSNNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240
           A A AP++SSSTSCGQPL DGLCPEWVYDDDKTLL+DMVGKGADMVVAQDGSGDFRTISE
Sbjct: 181 A-AVAPASSSSTSCGQPLPDGLCPEWVYDDDKTLLRDMVGKGADMVVAQDGSGDFRTISE 240

Query: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300
           AVAAAEEARKGSGS RFVIYVKGGIYKENVVI+K MKN+MM+GDGMDRTI+TAMKNVQDG
Sbjct: 241 AVAAAEEARKGSGSERFVIYVKGGIYKENVVIKKSMKNVMMIGDGMDRTIITAMKNVQDG 300

Query: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360
           STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA
Sbjct: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360

Query: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420
           HSNRQFYRECHIYGTVDFIFGDG VVFQNCNIFVRKPM+NQKNTITAQ RSDPNEN+GFV
Sbjct: 361 HSNRQFYRECHIYGTVDFIFGDGAVVFQNCNIFVRKPMMNQKNTITAQGRSDPNENTGFV 420

Query: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480
           IHNSVI AAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSY+DGLIEPAGWLPWAGDFGLRT
Sbjct: 421 IHNSVIKAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYLDGLIEPAGWLPWAGDFGLRT 480

Query: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           +YYGEFMN G GANTKGRV WPGYHVMTSA EAE FTVESFL G GWIP TGVPFVNG+
Sbjct: 481 IYYGEFMNIGGGANTKGRVRWPGYHVMTSATEAEGFTVESFLGGRGWIPTTGVPFVNGV 534

BLAST of CsGy5G018950 vs. NCBI nr
Match: XP_023549991.1 (pectinesterase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 825.5 bits (2131), Expect = 1.1e-235
Identity = 409/539 (75.88%), Postives = 457/539 (84.79%), Query Frame = 0

Query: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60
           M +LHCFLLFS L I H +SF+NFMSCSQTPYPDLCFH+INPN N+ T  + ++Y ITRF
Sbjct: 1   MNMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFINPNHNVPTTDVHQSYNITRF 60

Query: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120
           RNSAI+ATL QAM    HL S MDL SFDD+DR   ALIDCLE YEDSI ELNRS ++S 
Sbjct: 61  RNSAIRATLNQAMVAHHHLLSAMDLASFDDDDRVKSALIDCLELYEDSITELNRSVMNSM 120

Query: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180
            ANSIDH T+LSASLANHQTC DGFRDFGF     NN   P QI+SNFSKL++NSLAITK
Sbjct: 121 RANSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLITNSLAITK 180

Query: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240
           A AA  +T+SS    Q LL G  P+WV  DD+ LL+D   K ADMVVAQDGSG+FRTISE
Sbjct: 181 A-AAVTATTSSNPRSQRLLAGSFPKWVSSDDRMLLRDTTEKRADMVVAQDGSGNFRTISE 240

Query: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300
           AVAAA E RKGS   RFVIYVKGGIY+ENVVI+K +KN+MM+GDGM  TIVTAMKNV+DG
Sbjct: 241 AVAAAAEVRKGS-ERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDG 300

Query: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360
           STTF+SATFAV GEGFIAKDMTFEN AG EKHQAV+LR+NADRSIFYRCSFKGYQDTLYA
Sbjct: 301 STTFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYA 360

Query: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420
           HSNR F+RECHIYGT+DFIFGDG  VFQNCNIFVRKPM NQKNT+TAQ RSDPNEN+GFV
Sbjct: 361 HSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFV 420

Query: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480
           IHNSV+ AAPDLK V+G+Y TYLGRPWKAYSRTV+M+S++DGLIEPAGWLPWAGDFGL+T
Sbjct: 421 IHNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKT 480

Query: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           VYYGEF N+G GA+T  RV+WPGYHVM +A EAERFTVESFLEGGGWIPATGVPF NG+
Sbjct: 481 VYYGEFKNSGGGASTMRRVQWPGYHVMKTAAEAERFTVESFLEGGGWIPATGVPFANGV 537

BLAST of CsGy5G018950 vs. NCBI nr
Match: XP_022939088.1 (pectinesterase [Cucurbita moschata])

HSP 1 Score: 822.0 bits (2122), Expect = 1.2e-234
Identity = 408/539 (75.70%), Postives = 456/539 (84.60%), Query Frame = 0

Query: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60
           M++LHCFLLFS L I H +SF+NFMSCSQTPYPDLCFH+I PN N+ T  + ++Y ITRF
Sbjct: 1   MEMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRF 60

Query: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120
           RNSAI+ATL QAM    HL S+MDL SF D+DR   ALIDCLE YEDSI ELNRS ++S 
Sbjct: 61  RNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSM 120

Query: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180
            ANSI H T+LSASLANHQTC DGFRDFGF     NN   P QI+SNFSKL+SNSLAITK
Sbjct: 121 KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK 180

Query: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240
           A AA  +T+SST   Q LL G  P+WV  DD+ LL+D   K  DMVVAQDGSG+FRTISE
Sbjct: 181 A-AAVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISE 240

Query: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300
           AVAAA E RKGS   RFVIYVKGGIY+ENVVI+K +KN+MM+GDGM  TIVTAMKNV+DG
Sbjct: 241 AVAAAAEVRKGS-ERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDG 300

Query: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360
           STTF+SATFAV GEGFIAKDMTFEN AG EKHQAVALR+NADRSIFYRCSFKGYQDTLYA
Sbjct: 301 STTFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYA 360

Query: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420
           HSNR F+RECHIYGT+DFIFGDG  VFQNCNIFVRKPM NQKNT+TAQ RSDPNEN+GFV
Sbjct: 361 HSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFV 420

Query: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480
           IHNSV+ AAPDLK V+G+Y TYLGRPWKAYSRTV+M+S++DGLIEPAGWLPWAGDFGL+T
Sbjct: 421 IHNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKT 480

Query: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           VYYGEF N+G GA+T  RV+WPGYHVMT+A EAERFTVESFLEGGGWIP TGVPF NG+
Sbjct: 481 VYYGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANGV 537

BLAST of CsGy5G018950 vs. NCBI nr
Match: XP_022973540.1 (pectinesterase [Cucurbita maxima])

HSP 1 Score: 803.5 bits (2074), Expect = 4.3e-229
Identity = 404/537 (75.23%), Postives = 451/537 (83.99%), Query Frame = 0

Query: 3   ILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRN 62
           +L CFLLFS L I H +SF+ FMSCSQTPYPDLCFH+INPN NI T  + ++Y ITRFRN
Sbjct: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60

Query: 63  SAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSA 122
           SAI+ATL QAM    HL S MDL SFDD+ R   ALIDCLE YEDSI ELNRS ++S  A
Sbjct: 61  SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA 120

Query: 123 NSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAI 182
           +SIDH T+LSASLANHQTC DGFRDFGF     N  F P QI+SNFSKL+SNSLAITK I
Sbjct: 121 HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNIIF-PHQILSNFSKLISNSLAITK-I 180

Query: 183 AAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAV 242
           AA  +T+SST   Q +L G  P+WV  DD+ LL+D   K ADMVVAQDGSG+FRTISEAV
Sbjct: 181 AAVTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAV 240

Query: 243 AAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGST 302
           AAA E RK S   RFVIYVKGGIY+ENVVI+K +KN+MM+GDGM  TIVTAMKNV+DGST
Sbjct: 241 AAAAEVRKAS-ERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGST 300

Query: 303 TFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHS 362
           TF+SATFAV GEGFIAKDMTFEN AGPEKHQAVALR+NADRSIFYRCSFKGYQDTLYAHS
Sbjct: 301 TFRSATFAVVGEGFIAKDMTFENRAGPEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHS 360

Query: 363 NRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIH 422
           NR F+RECHIYGT+DFIFGDG  VFQNCNIFVRKPM NQKNT+TAQARSDPNEN+GFVIH
Sbjct: 361 NRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVIH 420

Query: 423 NSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVY 482
           NSV+ AAPDLK V+G+Y TYLGRPWKAYSRTV+M++ +DGLIEPAGWLPW GDFGL+TVY
Sbjct: 421 NSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRNVLDGLIEPAGWLPWGGDFGLKTVY 480

Query: 483 YGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           YGEF N+G GA+T  RV+WPGYHVM +  EAERFTVESFLEGGGWIP TGVPF NG+
Sbjct: 481 YGEFKNSGGGASTMRRVQWPGYHVMKTEAEAERFTVESFLEGGGWIPGTGVPFGNGV 534

BLAST of CsGy5G018950 vs. TAIR10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 482.3 bits (1240), Expect = 4.0e-136
Identity = 273/556 (49.10%), Postives = 359/556 (64.57%), Query Frame = 0

Query: 6   CFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAI 65
           C +    +V I   + +   SC QTPYP +C H+++ N  ++T   D+T   T F +  +
Sbjct: 17  CIITIIYVVSISHLNAHFITSCKQTPYPSVCDHHMS-NSPLKTLD-DQTDGFT-FHDLVV 76

Query: 66  QATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANS- 125
            +T+ QA+++   + S     S   +  +  AL DCLE YED+I +LN S  S    +S 
Sbjct: 77  SSTMDQAVQLHRLVSSLKQHHSLHKHATS--ALFDCLELYEDTIDQLNHSRRSYGQYSSP 136

Query: 126 IDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAA 185
            D  T LSA++AN  TC +GFRD  F +  S + + P+Q   N +K +SNSLA+TKA A 
Sbjct: 137 HDRQTSLSAAIANQDTCRNGFRD--FKLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAE 196

Query: 186 A-------PSTS--------SSTSCGQP-----LLDGLCPEWVYDDDKTLLQD-MVGKGA 245
           A       PST         SS   G         D   P W    D+ LL+D      A
Sbjct: 197 AEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKA 256

Query: 246 DMVVAQDGSGDFRTISEAVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVG 305
           D+VVA+DGSG + +I +AV AA  A+    + R VIYVK G+Y+ENVVI+K +KN+M++G
Sbjct: 257 DLVVAKDGSGHYTSIQQAVNAA--AKLPRRNQRLVIYVKAGVYRENVVIKKSIKNVMVIG 316

Query: 306 DGMDRTIVTAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADR 365
           DG+D TIVT  +NVQDG+TTF+SATFAV+G GFIA+ +TFENTAGPEKHQAVALRS++D 
Sbjct: 317 DGIDSTIVTGNRNVQDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDF 376

Query: 366 SIFYRCSFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKN 425
           S+FY CSFKGYQDTLY HS+RQF R C+IYGTVDFIFGD   +        RKPM  QKN
Sbjct: 377 SVFYACSFKGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAIXXXXXXXARKPMSGQKN 436

Query: 426 TITAQARSDPNENSGFVIHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGL 485
           TITAQ+R +P+E +GFVI +S +  A +         TYLGRPW+++SRTV MK  +  L
Sbjct: 437 TITAQSRKEPDETTGFVIQSSTVATASE---------TYLGRPWRSHSRTVFMKCNLGAL 496

Query: 486 IEPAGWLPWAGDFGLRTVYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLE 540
           + PAGWLPW+G F L T+YYGE+ NTG GA+  GRV+WPGYHV+ +  EAE+FTVE+FL+
Sbjct: 497 VSPAGWLPWSGSFALSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVENFLD 554

BLAST of CsGy5G018950 vs. TAIR10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 452.2 bits (1162), Expect = 4.4e-127
Identity = 246/513 (47.95%), Postives = 328/513 (63.94%), Query Frame = 0

Query: 27  CSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQAMKVRDHLRSTMDLG 86
           CSQTP P  C +++  N N      +  +L       +++  L +A+  + H      LG
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFL-----KISMKLVLDRAILAKTH---AFTLG 93

Query: 87  SFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSIDHSTMLSASLANHQTCLDGFR 146
               + R   A  DC++ Y+ +++++N +   +   + +D  T LS +L N  TC  GF 
Sbjct: 94  PKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFL 153

Query: 147 DFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAAAPSTSSSTSCGQPLLDGLCPEW 206
           + G       +  LP+ + +N S L+ N+LAI K            +   P  DG  P W
Sbjct: 154 ELGV-----TDIVLPL-MSNNVSNLLCNTLAINKV---------PFNYTPPEKDGF-PSW 213

Query: 207 VYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAVAAAEEARKGSGSGRFVIYVKGGIY 266
           V   D+ LLQ    K  + VVA+DGSG+F+TI EA+ AA      SGSGRFVIYVK G+Y
Sbjct: 214 VKPGDRKLLQSSTPKD-NAVVAKDGSGNFKTIKEAIDAA------SGSGRFVIYVKQGVY 273

Query: 267 KENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFENT 326
            EN  +E R KN+M+ GDG+ +TI+T  K+V  G+TTF SAT A  G+GFIA+ +TF NT
Sbjct: 274 SEN--LEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 333

Query: 327 AGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVVV 386
           AG    QAVALRS +D S+FY+CSF+ YQDTLY HSNRQFYR+C +YGTVDFIFG+   V
Sbjct: 334 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 393

Query: 387 FQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAAPDLKPVQGLYGTYLGRP 446
            QNCNIF R+P  ++ NTITAQ RSDPN+N+G +IHNS + AA DL+PV G   TYLGRP
Sbjct: 394 LQNCNIFARRPR-SKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRP 453

Query: 447 WKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGDGANTKGRVEWPGYHV 506
           W+ YSRTV MK+ +D LI+P GWL W G+F L+T++Y EF NTG GA+T GRV WPG+ V
Sbjct: 454 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 511

Query: 507 MTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           + SA EA +FTV +FL GG WIP++ VPF +GL
Sbjct: 514 LGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of CsGy5G018950 vs. TAIR10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 424.1 bits (1089), Expect = 1.3e-118
Identity = 236/552 (42.75%), Postives = 332/552 (60.14%), Query Frame = 0

Query: 1   MKILHCFLLFSLLVIIHGRSF----NNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYL 60
           + IL   L+  +L+ +H  +     N+   C +TPYPD C  Y   ++  +     +   
Sbjct: 8   LSILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQ-----QPTQ 67

Query: 61  ITRFRNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRS- 120
           ++ FR   ++A + +A+  R  L ++   G    + +    L DC++ Y D+I +LNR+ 
Sbjct: 68  LSEFRVMLVEAAMDRAISARAELTNS---GKNCTDSKKQAVLADCIDLYGDTIMQLNRTL 127

Query: 121 ------TLSSTSANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFS 180
                   ++ S    D  T LS +L N +TC  G  D      +  +F  PI   +  S
Sbjct: 128 HGVSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDL-----NVTDFITPIVSNTKIS 187

Query: 181 KLVSNSLAITKAIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQ 240
            L+SN LA+  A+  A +  ++T+  +       P W+   DK LL+ +    A++VVA+
Sbjct: 188 HLISNCLAVNGALLTAGNKGNTTANQKGF-----PTWLSRKDKRLLRAV---RANLVVAK 247

Query: 241 DGSGDFRTISEAVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRT 300
           DGSG F T+  A+  A   R+   SGRFVIYVK GIY+EN+ +     +IM+VGDGM  T
Sbjct: 248 DGSGHFNTVQAAIDVA--GRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRST 307

Query: 301 IVTAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRC 360
           I+T  ++VQ G TT+ SAT  + G  FIAK +TF NTAGP K QAVALRS++D SIFY+C
Sbjct: 308 IITGGRSVQGGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKC 367

Query: 361 SFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQA 420
           S +GYQDTL  HS RQFYREC+IYGTVDFIFG+   VFQNC I  R+P+  Q N ITAQ 
Sbjct: 368 SIEGYQDTLMVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQG 427

Query: 421 RSDPNENSGFVIHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGW 480
           R+DP +N+G  IHNS I  APDLKPV G   TY+GRPW  +SRTV++++Y+D ++ P GW
Sbjct: 428 RADPFQNTGISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGW 487

Query: 481 LPW--AGDFGLRTVYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGW 540
            PW     FGL T++Y E+ NTG  ++T+ RV W G+HV+  A +A  FTV  F+ G  W
Sbjct: 488 SPWIEGSVFGLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAW 536

BLAST of CsGy5G018950 vs. TAIR10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F)

HSP 1 Score: 416.8 bits (1070), Expect = 2.1e-116
Identity = 243/528 (46.02%), Postives = 318/528 (60.23%), Query Frame = 0

Query: 26  SCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQAMKVRDHLRSTMDL 85
           +CS++ YP+LC   +       TA  DE  LI    + +  ATL +  K   +  ST+  
Sbjct: 78  TCSKSLYPNLCIDTLLDFPGSLTA--DENELI----HISFNATLQKFSKAL-YTSSTITY 137

Query: 86  GSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSIDHS-----TMLSASLANHQT 145
                  R+  A   CLE  +DS+  L R+ LSS    S D S     T LS+++ NH T
Sbjct: 138 TQMPPRVRS--AYDSCLELLDDSVDALTRA-LSSVVVVSGDESHSDVMTWLSSAMTNHDT 197

Query: 146 CLDGFRDF---GFLVDDSNNFFLPIQIMSNFSKLVSNSLAI----TKAIAAAPSTSSSTS 205
           C DGF +    G  V D       I  + + S++VSN LAI     K ++  P  ++   
Sbjct: 198 CTDGFDEIEGQGGEVKDQ-----VIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKL 257

Query: 206 CGQPLLDGLCPEWVYDDDKTLLQDMVGK-GADMVVAQDGSGDFRTISEAVAAAEEARKGS 265
            G    + L P W+  +D+ LL        AD+ V++DGSG F+TI+EA+  A E     
Sbjct: 258 LGTEETEEL-PNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPE----H 317

Query: 266 GSGRFVIYVKGGIY-KENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATFAV 325
            S RFVIYVK G Y +EN+ + ++  N+M +GDG  +T++T  K++ D  TTF +ATFA 
Sbjct: 318 SSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAA 377

Query: 326 AGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYRECH 385
            G GFI +DMTFEN AGP KHQAVALR   D ++ YRC+  GYQD LY HSNRQF+REC 
Sbjct: 378 TGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECE 437

Query: 386 IYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAAPD 445
           IYGTVDFIFG+  V+ Q+CNI+ RKPM  QK TITAQ R DPN+N+G  IH   + A PD
Sbjct: 438 IYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPD 497

Query: 446 LKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGD 505
           L+  +G Y TYLGRPWK YSR V M S M   I+P GWL W G F L ++YYGE+MN G 
Sbjct: 498 LEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGL 557

Query: 506 GANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           G+    RV+WPGYHV+TS VEA +FTV  F+ G  W+P+TGV F +GL
Sbjct: 558 GSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

BLAST of CsGy5G018950 vs. TAIR10
Match: AT3G60730.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 415.2 bits (1066), Expect = 6.0e-116
Identity = 218/456 (47.81%), Postives = 284/456 (62.28%), Query Frame = 0

Query: 96  VALIDCLEFYEDSIAELNRSTLSSTSANSIDHSTMLSASLANHQTCLDGF---------- 155
           + L +C + Y++S A L++  +   +    D  T LS  LANH TCLDG           
Sbjct: 67  LGLSECEKLYDESEARLSKLVVDHENFTVEDVRTWLSGVLANHHTCLDGLIQQRQGHKPL 126

Query: 156 --RDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAAAPSTSSSTSCGQPLLDGLC 215
              +  F++ ++  F+   +   +  K +                               
Sbjct: 127 VHSNVTFVLHEALAFYK--KSRGHMKKRLHGXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 186

Query: 216 PEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAVAAAEEARKGSGSGRFVIYVKG 275
                +    +  +     AD VVA+DGS   RTI++A+AA     K S   R +IY+K 
Sbjct: 187 XXXXQNGGMLVSWNPTSSRADFVVARDGSATHRTINQALAAVSRMGK-SRLNRVIIYIKA 246

Query: 276 GIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATFAVAGEGFIAKDMTF 335
           G+Y E + I++ MKNIM+VGDGMDRTIVT  +NV DGSTT+ SATF V+G+GF A+D+TF
Sbjct: 247 GVYNEKIEIDRHMKNIMLVGDGMDRTIVTNNRNVPDGSTTYGSATFGVSGDGFWARDITF 306

Query: 336 ENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDG 395
           ENTAGP KHQAVALR ++D S+FYRCSFKGYQDTL+ HS RQFYR+CHIYGT+DFIFGD 
Sbjct: 307 ENTAGPHKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDA 366

Query: 396 VVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAAPDLKPVQGLYGTYL 455
             VFQNC+IFVR+PM +Q N ITAQ R DP+ NSG  I +S I AAP+ + V+G + +YL
Sbjct: 367 AAVFQNCDIFVRRPMDHQGNMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYL 426

Query: 456 GRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGDGANTKGRVEWPG 515
           GRPWK YSRTV +K+ +D LI+P GW  W+G + L T+YYGEFMNTG GA T  RV WPG
Sbjct: 427 GRPWKKYSRTVFLKTDIDELIDPRGWREWSGSYALSTLYYGEFMNTGAGAGTGRRVNWPG 486

Query: 516 YHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           +HV+    EA  FTV  F++G  WIP TGVPF  G+
Sbjct: 487 FHVLRGEEEASPFTVSRFIQGDSWIPITGVPFSAGV 519

BLAST of CsGy5G018950 vs. Swiss-Prot
Match: sp|O49298|PME6_ARATH (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PME6 PE=2 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 7.2e-135
Identity = 273/556 (49.10%), Postives = 359/556 (64.57%), Query Frame = 0

Query: 6   CFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAI 65
           C +    +V I   + +   SC QTPYP +C H+++ N  ++T   D+T   T F +  +
Sbjct: 17  CIITIIYVVSISHLNAHFITSCKQTPYPSVCDHHMS-NSPLKTLD-DQTDGFT-FHDLVV 76

Query: 66  QATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANS- 125
            +T+ QA+++   + S     S   +  +  AL DCLE YED+I +LN S  S    +S 
Sbjct: 77  SSTMDQAVQLHRLVSSLKQHHSLHKHATS--ALFDCLELYEDTIDQLNHSRRSYGQYSSP 136

Query: 126 IDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAA 185
            D  T LSA++AN  TC +GFRD  F +  S + + P+Q   N +K +SNSLA+TKA A 
Sbjct: 137 HDRQTSLSAAIANQDTCRNGFRD--FKLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAE 196

Query: 186 A-------PSTS--------SSTSCGQP-----LLDGLCPEWVYDDDKTLLQD-MVGKGA 245
           A       PST         SS   G         D   P W    D+ LL+D      A
Sbjct: 197 AEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKA 256

Query: 246 DMVVAQDGSGDFRTISEAVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVG 305
           D+VVA+DGSG + +I +AV AA  A+    + R VIYVK G+Y+ENVVI+K +KN+M++G
Sbjct: 257 DLVVAKDGSGHYTSIQQAVNAA--AKLPRRNQRLVIYVKAGVYRENVVIKKSIKNVMVIG 316

Query: 306 DGMDRTIVTAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADR 365
           DG+D TIVT  +NVQDG+TTF+SATFAV+G GFIA+ +TFENTAGPEKHQAVALRS++D 
Sbjct: 317 DGIDSTIVTGNRNVQDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDF 376

Query: 366 SIFYRCSFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKN 425
           S+FY CSFKGYQDTLY HS+RQF R C+IYGTVDFIFGD   +        RKPM  QKN
Sbjct: 377 SVFYACSFKGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAIXXXXXXXARKPMSGQKN 436

Query: 426 TITAQARSDPNENSGFVIHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGL 485
           TITAQ+R +P+E +GFVI +S +  A +         TYLGRPW+++SRTV MK  +  L
Sbjct: 437 TITAQSRKEPDETTGFVIQSSTVATASE---------TYLGRPWRSHSRTVFMKCNLGAL 496

Query: 486 IEPAGWLPWAGDFGLRTVYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLE 540
           + PAGWLPW+G F L T+YYGE+ NTG GA+  GRV+WPGYHV+ +  EAE+FTVE+FL+
Sbjct: 497 VSPAGWLPWSGSFALSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVENFLD 554

BLAST of CsGy5G018950 vs. Swiss-Prot
Match: sp|O04887|PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 1.5e-132
Identity = 260/533 (48.78%), Postives = 340/533 (63.79%), Query Frame = 0

Query: 8   LLFSLLVIIHGRSFNNFMS-CSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQ 67
           +LFSL     G S     S C +TP P  C +++    ++ +   D     T F   ++Q
Sbjct: 12  VLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQD-----TDFYKISLQ 71

Query: 68  ATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSID 127
             L +A   +     T  LGS   N+R   A  DC E YE ++ +LN+++ SS     +D
Sbjct: 72  LALERATTAQSR---TYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVD 131

Query: 128 HSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAAAP 187
             T LS +L N +TC     D G        + LP+ + +N +KL+SN+L++ K     P
Sbjct: 132 KQTWLSTALTNLETCRASLEDLGV-----PEYVLPL-LSNNVTKLISNTLSLNKVPYNEP 191

Query: 188 STSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAVAAAE 247
           S            DG  P WV   D+ LLQ      A++VVAQDGSG+ +TI EAVAAA 
Sbjct: 192 SYK----------DGF-PTWVKPGDRKLLQ--TTPRANIVVAQDGSGNVKTIQEAVAAAS 251

Query: 248 EARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQS 307
            A    G  R+VIY+K G Y EN  IE ++KNIM VGDG+ +TI+T  K+V  G+TTF+S
Sbjct: 252 RA----GGSRYVIYIKAGTYNEN--IEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKS 311

Query: 308 ATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQF 367
           AT AV G+ FIA+D+T  NTAGP  HQAVALRS +D S+FYRCSF+GYQDTLY HS RQF
Sbjct: 312 ATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQF 371

Query: 368 YRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVI 427
           YREC IYGTVDFIFG+  VV QNCNIF RKP  N+ NT+TAQ R+DPN+++G +IHN  +
Sbjct: 372 YRECDIYGTVDFIFGNAAVVLQNCNIFARKPP-NRTNTLTAQGRTDPNQSTGIIIHNCRV 431

Query: 428 GAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEF 487
            AA DLKPVQ    T+LGRPWK YSRTV +K+++D LI PAGW+ W+GDF L T+YY E+
Sbjct: 432 TAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEY 491

Query: 488 MNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           MNTG G++T  RV+W GYHV+TS  +  +FTV +F+ G  W+PAT VPF +GL
Sbjct: 492 MNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of CsGy5G018950 vs. Swiss-Prot
Match: sp|O22149|PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 452.2 bits (1162), Expect = 8.0e-126
Identity = 246/513 (47.95%), Postives = 328/513 (63.94%), Query Frame = 0

Query: 27  CSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQAMKVRDHLRSTMDLG 86
           CSQTP P  C +++  N N      +  +L       +++  L +A+  + H      LG
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFL-----KISMKLVLDRAILAKTH---AFTLG 93

Query: 87  SFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSIDHSTMLSASLANHQTCLDGFR 146
               + R   A  DC++ Y+ +++++N +   +   + +D  T LS +L N  TC  GF 
Sbjct: 94  PKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFL 153

Query: 147 DFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAAAPSTSSSTSCGQPLLDGLCPEW 206
           + G       +  LP+ + +N S L+ N+LAI K            +   P  DG  P W
Sbjct: 154 ELGV-----TDIVLPL-MSNNVSNLLCNTLAINKV---------PFNYTPPEKDGF-PSW 213

Query: 207 VYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAVAAAEEARKGSGSGRFVIYVKGGIY 266
           V   D+ LLQ    K  + VVA+DGSG+F+TI EA+ AA      SGSGRFVIYVK G+Y
Sbjct: 214 VKPGDRKLLQSSTPKD-NAVVAKDGSGNFKTIKEAIDAA------SGSGRFVIYVKQGVY 273

Query: 267 KENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFENT 326
            EN  +E R KN+M+ GDG+ +TI+T  K+V  G+TTF SAT A  G+GFIA+ +TF NT
Sbjct: 274 SEN--LEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 333

Query: 327 AGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVVV 386
           AG    QAVALRS +D S+FY+CSF+ YQDTLY HSNRQFYR+C +YGTVDFIFG+   V
Sbjct: 334 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 393

Query: 387 FQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAAPDLKPVQGLYGTYLGRP 446
            QNCNIF R+P  ++ NTITAQ RSDPN+N+G +IHNS + AA DL+PV G   TYLGRP
Sbjct: 394 LQNCNIFARRPR-SKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRP 453

Query: 447 WKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGDGANTKGRVEWPGYHV 506
           W+ YSRTV MK+ +D LI+P GWL W G+F L+T++Y EF NTG GA+T GRV WPG+ V
Sbjct: 454 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 511

Query: 507 MTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           + SA EA +FTV +FL GG WIP++ VPF +GL
Sbjct: 514 LGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of CsGy5G018950 vs. Swiss-Prot
Match: sp|O04886|PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 6.0e-121
Identity = 247/530 (46.60%), Postives = 320/530 (60.38%), Query Frame = 0

Query: 26  SCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQAMKVRDHLRSTMDL 85
           SCS T YPDLCF  I            +  +I    N    A  +    ++  L+ T   
Sbjct: 72  SCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT--- 131

Query: 86  GSFDDNDRNHVALIDCLEFYEDSIAELNRS--------TLSSTSANSIDHSTMLSASLAN 145
              +   R  VAL DCLE  ++++ EL+++           S S ++ D  T++SA++ N
Sbjct: 132 ---NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 191

Query: 146 HQTCLDGFRDFGFLVDDSNNFFLPI--QIMSNFSKLVSNSLAITKAIAAAPSTSSSTSCG 205
             TCLDGF       DD+N            +  K+ SN+LA+ K +         TS  
Sbjct: 192 QGTCLDGFSH-----DDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNN 251

Query: 206 QPL------LDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAVAAAEEAR 265
           + L      +DG  P W+   D+ LLQ       + VVA DGSG+F+T++ AVAAA +  
Sbjct: 252 RKLTEETSTVDG-WPAWLSPGDRRLLQSS-SVTPNAVVAADGSGNFKTVAAAVAAAPQ-- 311

Query: 266 KGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATF 325
              G+ R++I +K G+Y+ENV + K+ KNIM +GDG  RTI+T  +NV DGSTTF+SAT 
Sbjct: 312 --GGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATA 371

Query: 326 AVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYRE 385
           AV GEGF+A+D+TF+NTAGP KHQAVALR  AD S FY C    YQDTLY HSNRQF+  
Sbjct: 372 AVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVN 431

Query: 386 CHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAA 445
           C I GTVDFIFG+   V QNC+I  RKP   QKN +TAQ R+DPN+N+G VI  S IGA 
Sbjct: 432 CLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGAT 491

Query: 446 PDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNT 505
            DLKPVQG + TYLGRPWK YSRTVIM+S +  LI PAGW  W G+F L T++YGE  N+
Sbjct: 492 SDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNS 551

Query: 506 GDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           G GA T GRV+W G+ V+TSA EA+ FT  SF+ G  W+ +TG PF  GL
Sbjct: 552 GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CsGy5G018950 vs. Swiss-Prot
Match: sp|P83948|PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.0e-120
Identity = 243/529 (45.94%), Postives = 319/529 (60.30%), Query Frame = 0

Query: 26  SCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQAMKVRDHLRSTMDL 85
           SCS T YPDLCF  I            +  +I    N    A  +    ++  L+ T   
Sbjct: 72  SCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT--- 131

Query: 86  GSFDDNDRNHVALIDCLEFYEDSIAELNRS--------TLSSTSANSIDHSTMLSASLAN 145
              +   R  VAL DCLE  ++++ EL+++           S S ++ D  T++SA++ N
Sbjct: 132 ---NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 191

Query: 146 HQTCLDGFRDFGFLVDDSNNFFLPI--QIMSNFSKLVSNSLAITKAIAAAPSTSSSTSCG 205
             TCLDGF       DD+N            +  K+ SN+LA+ K +         TS  
Sbjct: 192 QGTCLDGFSH-----DDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNN 251

Query: 206 QPLLDGLC-----PEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAVAAAEEARK 265
           + L++        P W+   D+ LLQ       ++VVA DGSG+F+T++ +VAAA +   
Sbjct: 252 RKLIEETSTVDGWPAWLSTGDRRLLQSS-SVTPNVVVAADGSGNFKTVAASVAAAPQ--- 311

Query: 266 GSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATFA 325
             G+ R++I +K G+Y+ENV + K+ KNIM +GDG  RTI+T  +NV DGSTTF+SAT A
Sbjct: 312 -GGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVA 371

Query: 326 VAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYREC 385
           V GEGF+A+D+TF+NTAGP KHQAVALR  AD S FY C    YQDTLY HSNRQF+  C
Sbjct: 372 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 431

Query: 386 HIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAAP 445
            I GTVDFIFG+   V QNC+I  RKP   QKN +TAQ R+DPN+N+G VI  S IGA  
Sbjct: 432 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATS 491

Query: 446 DLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNTG 505
           DLKPVQG + TYLGRPWK YSRTVIM+S +  +I PAGW  W G+F L T++YGE  N G
Sbjct: 492 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAG 551

Query: 506 DGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
            GA T GRV+W G+ V+TSA EA+ FT  SF+ G  W+ +TG PF  GL
Sbjct: 552 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CsGy5G018950 vs. TrEMBL
Match: tr|A0A0A0KUU2|A0A0A0KUU2_CUCSA (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G568300 PE=3 SV=1)

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60
           MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF
Sbjct: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60

Query: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120
           RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST
Sbjct: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120

Query: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180
           SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK
Sbjct: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240
           AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE
Sbjct: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240

Query: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300
           AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG
Sbjct: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300

Query: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360
           STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA
Sbjct: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360

Query: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420
           HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV
Sbjct: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420

Query: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480
           IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT
Sbjct: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480

Query: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL
Sbjct: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 539

BLAST of CsGy5G018950 vs. TrEMBL
Match: tr|A0A1S3CSS2|A0A1S3CSS2_CUCME (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103504549 PE=3 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 3.8e-282
Identity = 486/539 (90.17%), Postives = 505/539 (93.69%), Query Frame = 0

Query: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60
           MK ++CFLLFSLLVIIHGRSFNNFMSC QTPYPDLCFHYINPNDNI TA IDETYL TRF
Sbjct: 1   MKFVYCFLLFSLLVIIHGRSFNNFMSCFQTPYPDLCFHYINPNDNIPTADIDETYLNTRF 60

Query: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120
           RNSA+QATL QA KV DHLRS MDLGSFDD++RN VALIDCLEFYEDSIA+LNRS  SST
Sbjct: 61  RNSAVQATLNQARKVHDHLRSIMDLGSFDDDNRNKVALIDCLEFYEDSIAKLNRSVFSST 120

Query: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180
           S NSIDH TMLSASLANHQTCLDGFRDF    D+SNNFFLPIQIMSNFSKLVSNSLAITK
Sbjct: 121 SVNSIDHLTMLSASLANHQTCLDGFRDF----DNSNNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240
           A A AP++SSSTSCGQPL DGLCPEWVYDDDKTLL+DMVGKGADMVVAQDGSGDFRTISE
Sbjct: 181 A-AVAPASSSSTSCGQPLPDGLCPEWVYDDDKTLLRDMVGKGADMVVAQDGSGDFRTISE 240

Query: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300
           AVAAAEEARKGSGS RFVIYVKGGIYKENVVI+K MKN+MM+GDGMDRTI+TAMKNVQDG
Sbjct: 241 AVAAAEEARKGSGSERFVIYVKGGIYKENVVIKKSMKNVMMIGDGMDRTIITAMKNVQDG 300

Query: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360
           STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA
Sbjct: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360

Query: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420
           HSNRQFYRECHIYGTVDFIFGDG VVFQNCNIFVRKPM+NQKNTITAQ RSDPNEN+GFV
Sbjct: 361 HSNRQFYRECHIYGTVDFIFGDGAVVFQNCNIFVRKPMMNQKNTITAQGRSDPNENTGFV 420

Query: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480
           IHNSVI AAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSY+DGLIEPAGWLPWAGDFGLRT
Sbjct: 421 IHNSVIKAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYLDGLIEPAGWLPWAGDFGLRT 480

Query: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           +YYGEFMN G GANTKGRV WPGYHVMTSA EAE FTVESFL G GWIP TGVPFVNG+
Sbjct: 481 IYYGEFMNIGGGANTKGRVRWPGYHVMTSATEAEGFTVESFLGGRGWIPTTGVPFVNGV 534

BLAST of CsGy5G018950 vs. TrEMBL
Match: tr|A0A2P5CP02|A0A2P5CP02_PARAD (Pectinesterase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_136800 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 1.9e-164
Identity = 316/538 (58.74%), Postives = 394/538 (73.23%), Query Frame = 0

Query: 7   FLLFSLLVIIHGRSFN--NFMSCSQTPYPDLCFHY-INPNDNIRTAHIDETYLITRFRNS 66
           FLLF  L ++HG + N      C+QTPYP +C HY IN N+ + +   D+  +   FR+ 
Sbjct: 15  FLLFFPLHVVHGTNNNTVTISPCNQTPYPHVCKHYIINTNNYLPSTLDDQNSVDLSFRDL 74

Query: 67  AIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSAN 126
           A++ T+ QA+ V   L +T+D  SFD  +R  +A  DC+E Y+D++  LNRS  SS++ N
Sbjct: 75  ALKVTMEQAV-VAHRLVATVDSNSFD--ERGKMAWQDCIELYKDTVDLLNRSIESSSNIN 134

Query: 127 S--IDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKA 186
           +  +D  T LSAS ANHQTC +GF DF      S+ F     ++SNFSKL+SNSLAI K+
Sbjct: 135 NNPLDTLTWLSASFANHQTCRNGFADFEL---SSSRFEFFPSMLSNFSKLLSNSLAIHKS 194

Query: 187 IAAAPSTSSSTSCGQPLLDGLC--PEWVYDDDKTLLQDMVG--KGADMVVAQDGSGDFRT 246
            +    T+S     + LL      P W+    + LLQ   G  + AD+VVAQDGSG ++T
Sbjct: 195 FST--KTTSFIHGRRRLLSDRSPWPVWLSGASRKLLQSGAGATEAADIVVAQDGSGGYKT 254

Query: 247 ISEAVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNV 306
           ISEAV AA  A+ G GS R VI+VK G+YKENV I K +KN+M++GDG+D TIVT  +N 
Sbjct: 255 ISEAVEAA--AKLGGGSKRIVIHVKSGVYKENVEISKEIKNLMVIGDGIDATIVTGNRNQ 314

Query: 307 QDGSTTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDT 366
           QDGSTTFQSATFAV+G+GFIA+DMTFENTAGP+KHQAVALRS AD S+FYRCSFKGYQDT
Sbjct: 315 QDGSTTFQSATFAVSGDGFIAQDMTFENTAGPQKHQAVALRSGADHSVFYRCSFKGYQDT 374

Query: 367 LYAHSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENS 426
           LY +S RQFYR+C IYGTVDFIFGD V V QN NI+VRKPM  Q+NT+TAQAR+DPNEN+
Sbjct: 375 LYVYSQRQFYRDCDIYGTVDFIFGDAVAVIQNSNIYVRKPMSGQENTVTAQARTDPNENT 434

Query: 427 GFVIHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFG 486
           G +IHNS I A+ DL+PVQG + TYLGRPW+ YSRTVIMK+ +DGLI+PAGWLPW+G+F 
Sbjct: 435 GIIIHNSRITASSDLRPVQGSFETYLGRPWQKYSRTVIMKTGLDGLIDPAGWLPWSGNFA 494

Query: 487 LRTVYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPF 536
           L T+YY EFMN+G GA T GRV WPGY V+TSA EAE+FTV +FL GG WIPATGVPF
Sbjct: 495 LSTLYYAEFMNSGAGAGTGGRVNWPGYRVLTSAAEAEKFTVGNFLAGGSWIPATGVPF 542

BLAST of CsGy5G018950 vs. TrEMBL
Match: tr|A0A2P5EP86|A0A2P5EP86_9ROSA (Pectinesterase OS=Trema orientalis OX=63057 GN=TorRG33x02_168520 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.2e-164
Identity = 315/538 (58.55%), Postives = 395/538 (73.42%), Query Frame = 0

Query: 7   FLLFSLLVIIHGRSFNNFM--SCSQTPYPDLCFHY-INPNDNIRTAHIDETYLITRFRNS 66
           FLLF  L ++HG + N  +  SC+QTPYP +C HY IN N+ + +   D+  +   FR+ 
Sbjct: 15  FLLFFPLHVVHGTNNNTVIISSCNQTPYPHVCKHYIINTNNYLPSTLDDQNSVDLSFRDL 74

Query: 67  AIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSAN 126
           A+  T+ QA++    L +T+D  SFD  +R  +A  DC+E Y+D++  LNRS  SS++ N
Sbjct: 75  ALNVTMEQAVEAH-RLVATVDSTSFD--ERGKLAWQDCIELYKDTVDLLNRSIKSSSNIN 134

Query: 127 S--IDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKA 186
           +  +D  T LSASLANHQTC +GF DF      S++F     ++SNFSKL+SNSLAI K+
Sbjct: 135 NNPLDTLTWLSASLANHQTCRNGFADFEL---SSSHFEFFPSMLSNFSKLLSNSLAIHKS 194

Query: 187 IAAAPSTSSSTSCGQPLL--DGLCPEWVYDDDKTLLQDMVG--KGADMVVAQDGSGDFRT 246
            +    T+S     + LL      P W+    + LLQ   G  + AD+VVAQDGSG ++T
Sbjct: 195 FST--KTTSFIHGRRRLLSDQSTWPVWLSGASRKLLQSGAGGTRAADIVVAQDGSGGYKT 254

Query: 247 ISEAVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNV 306
           ISEAV AA  A+ G GS R VI+VK G+YKENV I K +KN+M++GDG+D TIVT   N 
Sbjct: 255 ISEAVEAA--AKLGGGSNRIVIHVKSGVYKENVEISKEIKNLMVIGDGIDATIVTGNGNQ 314

Query: 307 QDGSTTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDT 366
           QDGSTTF+SATFAV+G+GFIA+D+TFENTAGP+KHQAVALRS AD S+FYRCSFKGYQDT
Sbjct: 315 QDGSTTFRSATFAVSGDGFIAQDITFENTAGPQKHQAVALRSGADHSVFYRCSFKGYQDT 374

Query: 367 LYAHSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENS 426
           LY +S RQFYR+C IYGTVDFIFGD V V QN NI+VRKPM  QKNT+TAQAR+DPNEN+
Sbjct: 375 LYVYSQRQFYRDCDIYGTVDFIFGDAVAVIQNSNIYVRKPMSGQKNTVTAQARTDPNENT 434

Query: 427 GFVIHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFG 486
           G +IHNS I A+ DL+PVQG + TYLGRPW+ YSRTVIMK+ +DGLI+PAGWLPW+G+F 
Sbjct: 435 GIIIHNSRITASSDLRPVQGSFETYLGRPWQEYSRTVIMKTGLDGLIDPAGWLPWSGNFA 494

Query: 487 LRTVYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPF 536
           L T+YY EFMN+G GA T GRV WPGY V+TSA EA +FTV +FL GG WIPATGVPF
Sbjct: 495 LSTLYYAEFMNSGAGAGTGGRVNWPGYRVLTSAAEAGKFTVGNFLAGGSWIPATGVPF 542

BLAST of CsGy5G018950 vs. TrEMBL
Match: tr|F6HFF6|F6HFF6_VITVI (Pectinesterase OS=Vitis vinifera OX=29760 GN=VIT_01s0011g05380 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 8.0e-163
Identity = 312/539 (57.88%), Postives = 385/539 (71.43%), Query Frame = 0

Query: 3   ILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRN 62
           +LH    + +++ +         SCSQTPYP++C ++I   +   TA IDE      FR+
Sbjct: 5   LLHFLSAYFVVLYVRVVDGAPISSCSQTPYPEVCNYFI--GNYKPTAGIDEIQF--PFRD 64

Query: 63  SAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSA 122
             +  T+ QA ++   L S MDL S D  +R  +A  DCLE YE++I  +NRS  S + A
Sbjct: 65  RVLGVTMNQAKRLH-LLVSAMDLSSSD--ERTKLAWADCLELYENTIDLVNRSINSISPA 124

Query: 123 NSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAI 182
              D  T LSA++AN QTCLDGF DF    D   +F       SNFSKL+SNSLAI KA 
Sbjct: 125 VMFDSQTWLSAAIANQQTCLDGFIDFNPSSDQFQSFPSMSISTSNFSKLLSNSLAINKAA 184

Query: 183 AAAPS-TSSSTSCGQPLLDGLCPEWVYDDDKTLLQDM-VGKGADMVVAQDGSGDFRTISE 242
            +A S  S++ + G+ LL    P WV   D+ LLQ       AD+VVA DGSG+++TISE
Sbjct: 185 VSATSILSNNQAGGRRLLSNGFPTWVSAADRKLLQSSGAASRADIVVAHDGSGNYKTISE 244

Query: 243 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 302
           AVAA+ + R  SG+ RFVIYVK G+Y+ENV I+++MKNIM++GDG D TIVT  KNVQDG
Sbjct: 245 AVAASVKLR--SGTKRFVIYVKAGVYRENVEIKRKMKNIMIIGDGKDATIVTGNKNVQDG 304

Query: 303 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 362
           STTF+SATFAV+G GFIA+DMTFENTAGPEKHQAVALRS++D S+FY CSFKGYQDTLY 
Sbjct: 305 STTFRSATFAVSGHGFIARDMTFENTAGPEKHQAVALRSSSDGSVFYGCSFKGYQDTLYV 364

Query: 363 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 422
           H+ RQFYR C +YGTVDFIFGD V V QNCNI+VR+PM NQ N ITAQ RSD NEN+G  
Sbjct: 365 HTQRQFYRSCDVYGTVDFIFGDAVAVLQNCNIYVRRPMSNQANVITAQGRSDQNENTGIS 424

Query: 423 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 482
           IHNS + AAPDL+PVQ  + TYLGRPW+ YSRTV MK+ +DGLI P GW PW GDFGL T
Sbjct: 425 IHNSRVMAAPDLRPVQSRFKTYLGRPWRKYSRTVFMKTSLDGLIHPEGWSPWKGDFGLST 484

Query: 483 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           +YYGE+MNTG GA+T+GRV+W GYHV+TSA EA++FTV  FL G  WIP TGVP+ +GL
Sbjct: 485 LYYGEYMNTGSGASTRGRVKWRGYHVITSAAEADKFTVGRFLVGDSWIPTTGVPYASGL 534