CSPI05G19440 (gene) Wild cucumber (PI 183967)

BLAST of CSPI05G19440 vs. Swiss-Prot
Match: PME6_ARATH (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana GN=PME6 PE=2 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.6e-139
Identity = 278/556 (50.00%), Postives = 365/556 (65.65%), Query Frame = 1

Query: 6   CFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAI 65
           C +    +V I   + +   SC QTPYP +C H+++ N  ++T   D+T   T F +  +
Sbjct: 17  CIITIIYVVSISHLNAHFITSCKQTPYPSVCDHHMS-NSPLKTLD-DQTDGFT-FHDLVV 76

Query: 66  QATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSI 125
            +T+ QA+++   + S     S   +  +  AL DCLE YED+I +LN S  S    +S 
Sbjct: 77  SSTMDQAVQLHRLVSSLKQHHSLHKHATS--ALFDCLELYEDTIDQLNHSRRSYGQYSSP 136

Query: 126 -DHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAA 185
            D  T LSA++AN  TC +GFRDF   +  S + + P+Q   N +K +SNSLA+TKA A 
Sbjct: 137 HDRQTSLSAAIANQDTCRNGFRDFK--LTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAE 196

Query: 186 A-------PSTS--------SSTSCGQPPL-----DGLCPEWVYDDDKTLLQDMVGKG-A 245
           A       PST         SS   G         D   P W    D+ LL+D      A
Sbjct: 197 AEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKA 256

Query: 246 DMVVAQDGSGDFRTISEAVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVG 305
           D+VVA+DGSG + +I +AV AA  A+    + R VIYVK G+Y+ENVVI+K +KN+M++G
Sbjct: 257 DLVVAKDGSGHYTSIQQAVNAA--AKLPRRNQRLVIYVKAGVYRENVVIKKSIKNVMVIG 316

Query: 306 DGMDRTIVTAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADR 365
           DG+D TIVT  +NVQDG+TTF+SATFAV+G GFIA+ +TFENTAGPEKHQAVALRS++D 
Sbjct: 317 DGIDSTIVTGNRNVQDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDF 376

Query: 366 SIFYRCSFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKN 425
           S+FY CSFKGYQDTLY HS+RQF R C+IYGTVDFIFGD   + QNCNI+ RKPM  QKN
Sbjct: 377 SVFYACSFKGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQKN 436

Query: 426 TITAQARSDPNENSGFVIHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGL 485
           TITAQ+R +P+E +GFVI +S +  A +         TYLGRPW+++SRTV MK  +  L
Sbjct: 437 TITAQSRKEPDETTGFVIQSSTVATASE---------TYLGRPWRSHSRTVFMKCNLGAL 496

Query: 486 IEPAGWLPWAGDFGLRTVYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLE 540
           + PAGWLPW+G F L T+YYGE+ NTG GA+  GRV+WPGYHV+ +  EAE+FTVE+FL+
Sbjct: 497 VSPAGWLPWSGSFALSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVENFLD 554

BLAST of CSPI05G19440 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 8.7e-133
Identity = 260/533 (48.78%), Postives = 340/533 (63.79%), Query Frame = 1

Query: 8   LLFSLLVIIHGRSFNNFMS-CSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQ 67
           +LFSL     G S     S C +TP P  C +++    ++ +   D     T F   ++Q
Sbjct: 12  VLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQD-----TDFYKISLQ 71

Query: 68  ATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSID 127
             L +A   +     T  LGS   N+R   A  DC E YE ++ +LN+++ SS     +D
Sbjct: 72  LALERATTAQSR---TYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVD 131

Query: 128 HSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAAAP 187
             T LS +L N +TC     D G        + LP+ + +N +KL+SN+L++ K     P
Sbjct: 132 KQTWLSTALTNLETCRASLEDLGV-----PEYVLPL-LSNNVTKLISNTLSLNKVPYNEP 191

Query: 188 STSSSTSCGQPPLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAVAAAE 247
           S            DG  P WV   D+ LLQ      A++VVAQDGSG+ +TI EAVAAA 
Sbjct: 192 SYK----------DGF-PTWVKPGDRKLLQTT--PRANIVVAQDGSGNVKTIQEAVAAAS 251

Query: 248 EARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQS 307
            A    G  R+VIY+K G Y EN  IE ++KNIM VGDG+ +TI+T  K+V  G+TTF+S
Sbjct: 252 RA----GGSRYVIYIKAGTYNEN--IEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKS 311

Query: 308 ATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQF 367
           AT AV G+ FIA+D+T  NTAGP  HQAVALRS +D S+FYRCSF+GYQDTLY HS RQF
Sbjct: 312 ATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQF 371

Query: 368 YRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVI 427
           YREC IYGTVDFIFG+  VV QNCNIF RKP  N+ NT+TAQ R+DPN+++G +IHN  +
Sbjct: 372 YRECDIYGTVDFIFGNAAVVLQNCNIFARKPP-NRTNTLTAQGRTDPNQSTGIIIHNCRV 431

Query: 428 GAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEF 487
            AA DLKPVQ    T+LGRPWK YSRTV +K+++D LI PAGW+ W+GDF L T+YY E+
Sbjct: 432 TAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEY 491

Query: 488 MNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           MNTG G++T  RV+W GYHV+TS  +  +FTV +F+ G  W+PAT VPF +GL
Sbjct: 492 MNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of CSPI05G19440 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 453.8 bits (1166), Expect = 2.7e-126
Identity = 246/513 (47.95%), Postives = 328/513 (63.94%), Query Frame = 1

Query: 27  CSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQAMKVRDHLRSTMDLG 86
           CSQTP P  C +++  N N      +  +L       +++  L +A+  + H      LG
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFL-----KISMKLVLDRAILAKTH---AFTLG 93

Query: 87  SFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSIDHSTMLSASLANHQTCLDGFR 146
               + R   A  DC++ Y+ +++++N +   +   + +D  T LS +L N  TC  GF 
Sbjct: 94  PKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFL 153

Query: 147 DFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAAAPSTSSSTSCGQPPLDGLCPEW 206
           + G       +  LP+ + +N S L+ N+LAI K            +   P  DG  P W
Sbjct: 154 ELGV-----TDIVLPL-MSNNVSNLLCNTLAINKV---------PFNYTPPEKDGF-PSW 213

Query: 207 VYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAVAAAEEARKGSGSGRFVIYVKGGIY 266
           V   D+ LLQ    K  + VVA+DGSG+F+TI EA+ AA      SGSGRFVIYVK G+Y
Sbjct: 214 VKPGDRKLLQSSTPKD-NAVVAKDGSGNFKTIKEAIDAA------SGSGRFVIYVKQGVY 273

Query: 267 KENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFENT 326
            EN+ I K  KN+M+ GDG+ +TI+T  K+V  G+TTF SAT A  G+GFIA+ +TF NT
Sbjct: 274 SENLEIRK--KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 333

Query: 327 AGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVVV 386
           AG    QAVALRS +D S+FY+CSF+ YQDTLY HSNRQFYR+C +YGTVDFIFG+   V
Sbjct: 334 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 393

Query: 387 FQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAAPDLKPVQGLYGTYLGRP 446
            QNCNIF R+P  ++ NTITAQ RSDPN+N+G +IHNS + AA DL+PV G   TYLGRP
Sbjct: 394 LQNCNIFARRPR-SKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRP 453

Query: 447 WKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGDGANTKGRVEWPGYHV 506
           W+ YSRTV MK+ +D LI+P GWL W G+F L+T++Y EF NTG GA+T GRV WPG+ V
Sbjct: 454 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 511

Query: 507 MTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           + SA EA +FTV +FL GG WIP++ VPF +GL
Sbjct: 514 LGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of CSPI05G19440 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.7e-120
Identity = 246/530 (46.42%), Postives = 319/530 (60.19%), Query Frame = 1

Query: 26  SCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQAMKVRDHLRSTMDL 85
           SCS T YPDLCF  I            +  +I    N    A  +    ++  L+ T   
Sbjct: 72  SCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT--- 131

Query: 86  GSFDDNDRNHVALIDCLEFYEDSIAELNRST--------LSSTSANSIDHSTMLSASLAN 145
              +   R  VAL DCLE  ++++ EL+++           S S ++ D  T++SA++ N
Sbjct: 132 ---NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 191

Query: 146 HQTCLDGFRDFGFLVDDSNNFFLPIQI--MSNFSKLVSNSLAITKAIAAAPSTSSSTSCG 205
             TCLDGF       DD+N            +  K+ SN+LA+ K +         TS  
Sbjct: 192 QGTCLDGFSH-----DDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNN 251

Query: 206 QP------PLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAVAAAEEAR 265
           +        +DG  P W+   D+ LLQ       + VVA DGSG+F+T++ AVAAA +  
Sbjct: 252 RKLTEETSTVDGW-PAWLSPGDRRLLQSS-SVTPNAVVAADGSGNFKTVAAAVAAAPQG- 311

Query: 266 KGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATF 325
              G+ R++I +K G+Y+ENV + K+ KNIM +GDG  RTI+T  +NV DGSTTF+SAT 
Sbjct: 312 ---GTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATA 371

Query: 326 AVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYRE 385
           AV GEGF+A+D+TF+NTAGP KHQAVALR  AD S FY C    YQDTLY HSNRQF+  
Sbjct: 372 AVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVN 431

Query: 386 CHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAA 445
           C I GTVDFIFG+   V QNC+I  RKP   QKN +TAQ R+DPN+N+G VI  S IGA 
Sbjct: 432 CLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGAT 491

Query: 446 PDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNT 505
            DLKPVQG + TYLGRPWK YSRTVIM+S +  LI PAGW  W G+F L T++YGE  N+
Sbjct: 492 SDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNS 551

Query: 506 GDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           G GA T GRV+W G+ V+TSA EA+ FT  SF+ G  W+ +TG PF  GL
Sbjct: 552 GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CSPI05G19440 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 5.0e-120
Identity = 242/529 (45.75%), Postives = 318/529 (60.11%), Query Frame = 1

Query: 26  SCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQAMKVRDHLRSTMDL 85
           SCS T YPDLCF  I            +  +I    N    A  +    ++  L+ T   
Sbjct: 72  SCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT--- 131

Query: 86  GSFDDNDRNHVALIDCLEFYEDSIAELNRST--------LSSTSANSIDHSTMLSASLAN 145
              +   R  VAL DCLE  ++++ EL+++           S S ++ D  T++SA++ N
Sbjct: 132 ---NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 191

Query: 146 HQTCLDGFRDFGFLVDDSNNFFLPIQI--MSNFSKLVSNSLAITKAIAAAPSTSSSTSCG 205
             TCLDGF       DD+N            +  K+ SN+LA+ K +         TS  
Sbjct: 192 QGTCLDGFSH-----DDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNN 251

Query: 206 QPPLDGLC-----PEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAVAAAEEARK 265
           +  ++        P W+   D+ LLQ       ++VVA DGSG+F+T++ +VAAA +   
Sbjct: 252 RKLIEETSTVDGWPAWLSTGDRRLLQSS-SVTPNVVVAADGSGNFKTVAASVAAAPQG-- 311

Query: 266 GSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATFA 325
             G+ R++I +K G+Y+ENV + K+ KNIM +GDG  RTI+T  +NV DGSTTF+SAT A
Sbjct: 312 --GTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVA 371

Query: 326 VAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYREC 385
           V GEGF+A+D+TF+NTAGP KHQAVALR  AD S FY C    YQDTLY HSNRQF+  C
Sbjct: 372 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 431

Query: 386 HIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAAP 445
            I GTVDFIFG+   V QNC+I  RKP   QKN +TAQ R+DPN+N+G VI  S IGA  
Sbjct: 432 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATS 491

Query: 446 DLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNTG 505
           DLKPVQG + TYLGRPWK YSRTVIM+S +  +I PAGW  W G+F L T++YGE  N G
Sbjct: 492 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAG 551

Query: 506 DGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
            GA T GRV+W G+ V+TSA EA+ FT  SF+ G  W+ +TG PF  GL
Sbjct: 552 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CSPI05G19440 vs. TrEMBL
Match: A0A0A0KUU2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G568300 PE=4 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 538/539 (99.81%), Postives = 538/539 (99.81%), Query Frame = 1

Query: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60
           MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF
Sbjct: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60

Query: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120
           RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST
Sbjct: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120

Query: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180
           SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK
Sbjct: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 AIAAAPSTSSSTSCGQPPLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240
           AIAAAPSTSSSTSCGQP LDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE
Sbjct: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240

Query: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300
           AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG
Sbjct: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300

Query: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360
           STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA
Sbjct: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360

Query: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420
           HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV
Sbjct: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420

Query: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480
           IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT
Sbjct: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480

Query: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL
Sbjct: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 539

BLAST of CSPI05G19440 vs. TrEMBL
Match: F6HFF6_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_01s0011g05380 PE=4 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 2.1e-162
Identity = 311/539 (57.70%), Postives = 382/539 (70.87%), Query Frame = 1

Query: 3   ILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRN 62
           +LH    + +++ +         SCSQTPYP++C ++I   +   TA IDE      FR+
Sbjct: 5   LLHFLSAYFVVLYVRVVDGAPISSCSQTPYPEVCNYFIG--NYKPTAGIDEIQF--PFRD 64

Query: 63  SAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSA 122
             +  T+ QA ++   L S MDL S D+  R  +A  DCLE YE++I  +NRS  S + A
Sbjct: 65  RVLGVTMNQAKRLH-LLVSAMDLSSSDE--RTKLAWADCLELYENTIDLVNRSINSISPA 124

Query: 123 NSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAI 182
              D  T LSA++AN QTCLDGF DF    D   +F       SNFSKL+SNSLAI KA 
Sbjct: 125 VMFDSQTWLSAAIANQQTCLDGFIDFNPSSDQFQSFPSMSISTSNFSKLLSNSLAINKAA 184

Query: 183 AAAPSTSSSTSCG-QPPLDGLCPEWVYDDDKTLLQDM-VGKGADMVVAQDGSGDFRTISE 242
            +A S  S+   G +  L    P WV   D+ LLQ       AD+VVA DGSG+++TISE
Sbjct: 185 VSATSILSNNQAGGRRLLSNGFPTWVSAADRKLLQSSGAASRADIVVAHDGSGNYKTISE 244

Query: 243 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 302
           AVAA+ + R  SG+ RFVIYVK G+Y+ENV I+++MKNIM++GDG D TIVT  KNVQDG
Sbjct: 245 AVAASVKLR--SGTKRFVIYVKAGVYRENVEIKRKMKNIMIIGDGKDATIVTGNKNVQDG 304

Query: 303 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 362
           STTF+SATFAV+G GFIA+DMTFENTAGPEKHQAVALRS++D S+FY CSFKGYQDTLY 
Sbjct: 305 STTFRSATFAVSGHGFIARDMTFENTAGPEKHQAVALRSSSDGSVFYGCSFKGYQDTLYV 364

Query: 363 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 422
           H+ RQFYR C +YGTVDFIFGD V V QNCNI+VR+PM NQ N ITAQ RSD NEN+G  
Sbjct: 365 HTQRQFYRSCDVYGTVDFIFGDAVAVLQNCNIYVRRPMSNQANVITAQGRSDQNENTGIS 424

Query: 423 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 482
           IHNS + AAPDL+PVQ  + TYLGRPW+ YSRTV MK+ +DGLI P GW PW GDFGL T
Sbjct: 425 IHNSRVMAAPDLRPVQSRFKTYLGRPWRKYSRTVFMKTSLDGLIHPEGWSPWKGDFGLST 484

Query: 483 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           +YYGE+MNTG GA+T+GRV+W GYHV+TSA EA++FTV  FL G  WIP TGVP+ +GL
Sbjct: 485 LYYGEYMNTGSGASTRGRVKWRGYHVITSAAEADKFTVGRFLVGDSWIPTTGVPYASGL 534

BLAST of CSPI05G19440 vs. TrEMBL
Match: W9S5Z7_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_011995 PE=4 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 2.0e-160
Identity = 306/530 (57.74%), Postives = 383/530 (72.26%), Query Frame = 1

Query: 13  LVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQA 72
           + ++HG   +   +C+QTPYP +C H++N N     + +D+   ++ F + A++AT+ QA
Sbjct: 1   MYVVHGTK-STISTCNQTPYPQVCHHFLNTN---HLSTLDDKTTVS-FHDLALKATMKQA 60

Query: 73  MKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSIDHSTMLS 132
           ++    L STMD  SF   +R   A  DCLE Y+D++  LNRS ++S + N +D  T LS
Sbjct: 61  VEAH-RLVSTMDSDSF--GERGKSAWQDCLELYKDTVDLLNRS-VNSNNKNPLDTHTWLS 120

Query: 133 ASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAAAPSTSSST 192
           ASLAN  TC +GF DF      S+    P  ++SNFSKL+SNSLAI KA   + +TS S 
Sbjct: 121 ASLANQHTCRNGFTDFQI---PSHYDSFP-SMLSNFSKLLSNSLAINKATIPSTATSFSK 180

Query: 193 SCGQPPL--DGLCPEWVYDDDKTLLQDM-VGKGADMVVAQDGSGDFRTISEAVAAAEEAR 252
                 L  DG  PEWV   D+ LLQ       AD+VVAQDGSG F+TISEAV AA+   
Sbjct: 181 QVRSRRLLSDGF-PEWVSAADRKLLQSGGAAAKADIVVAQDGSGGFKTISEAVEAAKNL- 240

Query: 253 KGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATF 312
            G GS R V+YVK G+YKENV I+  +KN+M VGDG+D TIVT  +N  DGSTTFQSATF
Sbjct: 241 -GGGSKRIVVYVKAGVYKENVEIKNDIKNLMFVGDGIDATIVTGNRNNVDGSTTFQSATF 300

Query: 313 AVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYRE 372
            V+G+GFIA+DMTFENTAGP KHQAVALRS +D S+F+RCSFKGYQDTLY +S RQFYR+
Sbjct: 301 GVSGDGFIAQDMTFENTAGPAKHQAVALRSGSDHSVFHRCSFKGYQDTLYVYSQRQFYRD 360

Query: 373 CHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAA 432
           C++YGTVDFI GD V + QNCNI VRKPM +Q+NT+T+QAR+DPNEN+G +IHNS I A+
Sbjct: 361 CNVYGTVDFICGDAVAILQNCNILVRKPMSDQRNTVTSQARTDPNENTGIIIHNSRITAS 420

Query: 433 PDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNT 492
            DL+PVQG + TYLGRPW+ YSRTVIMKS +DGLI+P GWLPW+G+F L T+YY EFMN+
Sbjct: 421 SDLRPVQGSFETYLGRPWQKYSRTVIMKSSLDGLIDPKGWLPWSGNFALSTLYYAEFMNS 480

Query: 493 GDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           G GA T GRV WPGYHV+TSA EA +FTV +FL G  WIPATGVPF  GL
Sbjct: 481 GAGAATGGRVSWPGYHVLTSAAEAGKFTVGNFLGGDSWIPATGVPFAAGL 514

BLAST of CSPI05G19440 vs. TrEMBL
Match: B9HW40_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0010s11960g PE=4 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 5.8e-160
Identity = 299/542 (55.17%), Postives = 390/542 (71.96%), Query Frame = 1

Query: 4   LHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNS 63
           L+ + LF  L ++HG S     SC QTP+P+ C ++I+ N       I +T  +   R+ 
Sbjct: 11  LNAYFLFYSLALVHGDSVT---SCDQTPFPEACNYFIDTN-------ISKTPPLFALRDQ 70

Query: 64  AIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSAN 123
           ++  T+ +A++    + S+M+L SF  N +  +A  DCL+ YED++  +NRS  S+   N
Sbjct: 71  SLSITMNKAIEAHQMV-SSMELSSF--NQQAKLAWDDCLKLYEDTVDHVNRSMSSN---N 130

Query: 124 SIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIA 183
             D  T LSA++AN +TC +GF DF  +   S    LP  ++ NF+KL+SN+L++ KAI 
Sbjct: 131 LADSQTWLSAAIANQRTCENGFIDFNIV---SYLESLP-NMLRNFTKLLSNTLSLNKAII 190

Query: 184 AAP----STSSSTSCGQPPLDGLCPEWVYDDDKTLLQD--MVGKGADMVVAQDGSGDFRT 243
           ++      T       +  +DG  P WV   D+ LLQ        AD+VVAQDGSGD++T
Sbjct: 191 SSTPILLDTKQDGGRRRLLVDGF-PSWVPASDRKLLQSNGRAAPKADIVVAQDGSGDYKT 250

Query: 244 ISEAVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNV 303
           ISEAVAA+ + R G+ S RFVIYVKGG+YKENV I+K MKN+M VGDG+D T++T+ KN 
Sbjct: 251 ISEAVAASAKLRSGTKSKRFVIYVKGGVYKENVEIKKSMKNLMFVGDGIDATVITSNKNT 310

Query: 304 QDGSTTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDT 363
           QDG+TTF+SAT  V+G+GFIA+D+TFENTAGP+KHQAVALRS +D S+FY CSFKGYQDT
Sbjct: 311 QDGTTTFRSATVGVSGKGFIARDITFENTAGPQKHQAVALRSGSDFSVFYSCSFKGYQDT 370

Query: 364 LYAHSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENS 423
           LY HS RQFYR+C IYGTVDFIFGD V V QNCNI+VR+PM  Q NT+TAQ R+DP+EN+
Sbjct: 371 LYVHSQRQFYRDCDIYGTVDFIFGDAVAVLQNCNIYVRRPMSKQTNTVTAQGRTDPDENT 430

Query: 424 GFVIHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFG 483
           G VIHNS + AAPDL+PVQG + +YLGRPWK YSRTV +KS +DGLI+PAGWLPW GDF 
Sbjct: 431 GIVIHNSRVMAAPDLRPVQGSFKSYLGRPWKKYSRTVFLKSNIDGLIDPAGWLPWKGDFA 490

Query: 484 LRTVYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVN 540
           L T+YYGE+M+TG GA+TKGRV+WPGYH +TS +EA +FTVE+FL G  WI A GVPF +
Sbjct: 491 LSTLYYGEYMSTGSGASTKGRVKWPGYHTITSPLEAGKFTVENFLAGNSWISAAGVPFES 531

BLAST of CSPI05G19440 vs. TrEMBL
Match: A0A164SPZ1_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_023588 PE=4 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 2.5e-158
Identity = 312/538 (57.99%), Postives = 376/538 (69.89%), Query Frame = 1

Query: 4   LHCFLLFSLL-VIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRN 63
           L   LLF+L   ++HG + +    C+QTPYP+LC      N  + T         T  R 
Sbjct: 14  LKSLLLFTLCNAVVHGATTS---ICNQTPYPELC------NSFMVTTTTSNENQFTGLRE 73

Query: 64  SAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSA 123
           SAI ATL +A +  D + S MD+GSF+   ++  A  DCLE YEDS+ ++NRS  S  S 
Sbjct: 74  SAISATLDRAKQTYDAI-SAMDVGSFEARAKS--AWADCLELYEDSVHQINRSLNSDNSK 133

Query: 124 NSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAI 183
           N  D  T LSA+L +HQTC +GF DF  L    N+F  P    +N SK +SNSLAI KA 
Sbjct: 134 NENDIQTWLSAALTDHQTCKNGFLDFN-LNSYLNSF--PF-AETNISKFLSNSLAIHKAT 193

Query: 184 AAAPSTSSSTSCGQPPL-DGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEA 243
           AA   ++     G+  L D   PEW+   D+ LLQ      AD+VVAQDGSGD+ TISEA
Sbjct: 194 AATSLSNVKNIKGRRLLNDNEFPEWLTAADRRLLQ--AAPEADLVVAQDGSGDYTTISEA 253

Query: 244 VAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGS 303
           VA++ +    SGSGRFVIYVK G+YKENV  E  MKN+M+VGDGMD T+VT  KN QDG 
Sbjct: 254 VASSAQK---SGSGRFVIYVKSGVYKENV--EITMKNLMLVGDGMDATVVTGSKNSQDGI 313

Query: 304 TTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAH 363
           TTF++ATFA  G+GFIA DMTFENTAGP+KHQAVALRS AD S+FYRCSFKGYQDTLY  
Sbjct: 314 TTFKTATFATTGDGFIAMDMTFENTAGPQKHQAVALRSGADFSVFYRCSFKGYQDTLYVF 373

Query: 364 SNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVI 423
           S RQFYR C IYGT+DFIFG+ V V Q+CNI+VRKPM NQ NT+TAQ RSDPNEN+G +I
Sbjct: 374 SLRQFYRNCDIYGTIDFIFGNAVAVMQSCNIYVRKPMSNQFNTVTAQHRSDPNENTGIII 433

Query: 424 HNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTV 483
            NSV+  A DL+P QG + TYLGRPW  YSRTV+MKS +DG I P GW PW GDF L T+
Sbjct: 434 QNSVVTPASDLEPEQGSFKTYLGRPWGEYSRTVVMKSSLDGFISPQGWFPWDGDFALSTL 493

Query: 484 YYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           YYGE+MNTG GA+T GRV+WPGYHV+TSA EA +F+V +FL G  WIPATGVPF +GL
Sbjct: 494 YYGEYMNTGSGASTSGRVKWPGYHVITSAAEAGKFSVGNFLNGNSWIPATGVPFSSGL 528

BLAST of CSPI05G19440 vs. TAIR10
Match: AT1G23200.1 (AT1G23200.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 497.7 bits (1280), Expect = 9.3e-141
Identity = 278/556 (50.00%), Postives = 365/556 (65.65%), Query Frame = 1

Query: 6   CFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAI 65
           C +    +V I   + +   SC QTPYP +C H+++ N  ++T   D+T   T F +  +
Sbjct: 17  CIITIIYVVSISHLNAHFITSCKQTPYPSVCDHHMS-NSPLKTLD-DQTDGFT-FHDLVV 76

Query: 66  QATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSI 125
            +T+ QA+++   + S     S   +  +  AL DCLE YED+I +LN S  S    +S 
Sbjct: 77  SSTMDQAVQLHRLVSSLKQHHSLHKHATS--ALFDCLELYEDTIDQLNHSRRSYGQYSSP 136

Query: 126 -DHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAA 185
            D  T LSA++AN  TC +GFRDF   +  S + + P+Q   N +K +SNSLA+TKA A 
Sbjct: 137 HDRQTSLSAAIANQDTCRNGFRDFK--LTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAE 196

Query: 186 A-------PSTS--------SSTSCGQPPL-----DGLCPEWVYDDDKTLLQDMVGKG-A 245
           A       PST         SS   G         D   P W    D+ LL+D      A
Sbjct: 197 AEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKA 256

Query: 246 DMVVAQDGSGDFRTISEAVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVG 305
           D+VVA+DGSG + +I +AV AA  A+    + R VIYVK G+Y+ENVVI+K +KN+M++G
Sbjct: 257 DLVVAKDGSGHYTSIQQAVNAA--AKLPRRNQRLVIYVKAGVYRENVVIKKSIKNVMVIG 316

Query: 306 DGMDRTIVTAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADR 365
           DG+D TIVT  +NVQDG+TTF+SATFAV+G GFIA+ +TFENTAGPEKHQAVALRS++D 
Sbjct: 317 DGIDSTIVTGNRNVQDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDF 376

Query: 366 SIFYRCSFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKN 425
           S+FY CSFKGYQDTLY HS+RQF R C+IYGTVDFIFGD   + QNCNI+ RKPM  QKN
Sbjct: 377 SVFYACSFKGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQKN 436

Query: 426 TITAQARSDPNENSGFVIHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGL 485
           TITAQ+R +P+E +GFVI +S +  A +         TYLGRPW+++SRTV MK  +  L
Sbjct: 437 TITAQSRKEPDETTGFVIQSSTVATASE---------TYLGRPWRSHSRTVFMKCNLGAL 496

Query: 486 IEPAGWLPWAGDFGLRTVYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLE 540
           + PAGWLPW+G F L T+YYGE+ NTG GA+  GRV+WPGYHV+ +  EAE+FTVE+FL+
Sbjct: 497 VSPAGWLPWSGSFALSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVENFLD 554

BLAST of CSPI05G19440 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 453.8 bits (1166), Expect = 1.5e-127
Identity = 246/513 (47.95%), Postives = 328/513 (63.94%), Query Frame = 1

Query: 27  CSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQAMKVRDHLRSTMDLG 86
           CSQTP P  C +++  N N      +  +L       +++  L +A+  + H      LG
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFL-----KISMKLVLDRAILAKTH---AFTLG 93

Query: 87  SFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSIDHSTMLSASLANHQTCLDGFR 146
               + R   A  DC++ Y+ +++++N +   +   + +D  T LS +L N  TC  GF 
Sbjct: 94  PKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFL 153

Query: 147 DFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAAAPSTSSSTSCGQPPLDGLCPEW 206
           + G       +  LP+ + +N S L+ N+LAI K            +   P  DG  P W
Sbjct: 154 ELGV-----TDIVLPL-MSNNVSNLLCNTLAINKV---------PFNYTPPEKDGF-PSW 213

Query: 207 VYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAVAAAEEARKGSGSGRFVIYVKGGIY 266
           V   D+ LLQ    K  + VVA+DGSG+F+TI EA+ AA      SGSGRFVIYVK G+Y
Sbjct: 214 VKPGDRKLLQSSTPKD-NAVVAKDGSGNFKTIKEAIDAA------SGSGRFVIYVKQGVY 273

Query: 267 KENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFENT 326
            EN+ I K  KN+M+ GDG+ +TI+T  K+V  G+TTF SAT A  G+GFIA+ +TF NT
Sbjct: 274 SENLEIRK--KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 333

Query: 327 AGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVVV 386
           AG    QAVALRS +D S+FY+CSF+ YQDTLY HSNRQFYR+C +YGTVDFIFG+   V
Sbjct: 334 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 393

Query: 387 FQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAAPDLKPVQGLYGTYLGRP 446
            QNCNIF R+P  ++ NTITAQ RSDPN+N+G +IHNS + AA DL+PV G   TYLGRP
Sbjct: 394 LQNCNIFARRPR-SKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRP 453

Query: 447 WKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGDGANTKGRVEWPGYHV 506
           W+ YSRTV MK+ +D LI+P GWL W G+F L+T++Y EF NTG GA+T GRV WPG+ V
Sbjct: 454 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 511

Query: 507 MTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           + SA EA +FTV +FL GG WIP++ VPF +GL
Sbjct: 514 LGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of CSPI05G19440 vs. TAIR10
Match: AT5G51490.1 (AT5G51490.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 423.7 bits (1088), Expect = 1.7e-118
Identity = 236/552 (42.75%), Postives = 331/552 (59.96%), Query Frame = 1

Query: 1   MKILHCFLLFSLLVIIHGRSF----NNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYL 60
           + IL   L+  +L+ +H  +     N+   C +TPYPD C  Y   ++  +     +   
Sbjct: 8   LSILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQ-----QPTQ 67

Query: 61  ITRFRNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRST 120
           ++ FR   ++A + +A+  R  L ++   G    + +    L DC++ Y D+I +LNR+ 
Sbjct: 68  LSEFRVMLVEAAMDRAISARAELTNS---GKNCTDSKKQAVLADCIDLYGDTIMQLNRTL 127

Query: 121 L-------SSTSANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFS 180
                   ++ S    D  T LS +L N +TC  G  D         +F  PI   +  S
Sbjct: 128 HGVSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNV-----TDFITPIVSNTKIS 187

Query: 181 KLVSNSLAITKAIAAAPSTSSSTSCGQPPLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQ 240
            L+SN LA+  A+  A +  ++T+  +       P W+   DK LL+ +    A++VVA+
Sbjct: 188 HLISNCLAVNGALLTAGNKGNTTANQKG-----FPTWLSRKDKRLLRAV---RANLVVAK 247

Query: 241 DGSGDFRTISEAVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRT 300
           DGSG F T+  A+  A   R+   SGRFVIYVK GIY+EN+ +     +IM+VGDGM  T
Sbjct: 248 DGSGHFNTVQAAIDVA--GRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRST 307

Query: 301 IVTAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRC 360
           I+T  ++VQ G TT+ SAT  + G  FIAK +TF NTAGP K QAVALRS++D SIFY+C
Sbjct: 308 IITGGRSVQGGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKC 367

Query: 361 SFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQA 420
           S +GYQDTL  HS RQFYREC+IYGTVDFIFG+   VFQNC I  R+P+  Q N ITAQ 
Sbjct: 368 SIEGYQDTLMVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQG 427

Query: 421 RSDPNENSGFVIHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGW 480
           R+DP +N+G  IHNS I  APDLKPV G   TY+GRPW  +SRTV++++Y+D ++ P GW
Sbjct: 428 RADPFQNTGISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGW 487

Query: 481 LPW--AGDFGLRTVYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGW 540
            PW     FGL T++Y E+ NTG  ++T+ RV W G+HV+  A +A  FTV  F+ G  W
Sbjct: 488 SPWIEGSVFGLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAW 536

BLAST of CSPI05G19440 vs. TAIR10
Match: AT5G53370.1 (AT5G53370.1 pectin methylesterase PCR fragment F)

HSP 1 Score: 417.9 bits (1073), Expect = 9.3e-117
Identity = 243/528 (46.02%), Postives = 318/528 (60.23%), Query Frame = 1

Query: 26  SCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQAMKVRDHLRSTMDL 85
           +CS++ YP+LC   +       TA  DE  LI    + +  ATL +  K   +  ST+  
Sbjct: 78  TCSKSLYPNLCIDTLLDFPGSLTA--DENELI----HISFNATLQKFSKAL-YTSSTITY 137

Query: 86  GSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSIDHS-----TMLSASLANHQT 145
                  R+  A   CLE  +DS+  L R+ LSS    S D S     T LS+++ NH T
Sbjct: 138 TQMPPRVRS--AYDSCLELLDDSVDALTRA-LSSVVVVSGDESHSDVMTWLSSAMTNHDT 197

Query: 146 CLDGFRDF---GFLVDDSNNFFLPIQIMSNFSKLVSNSLAI----TKAIAAAPSTSSSTS 205
           C DGF +    G  V D       I  + + S++VSN LAI     K ++  P  ++   
Sbjct: 198 CTDGFDEIEGQGGEVKDQ-----VIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKL 257

Query: 206 CGQPPLDGLCPEWVYDDDKTLLQDMVGK-GADMVVAQDGSGDFRTISEAVAAAEEARKGS 265
            G    + L P W+  +D+ LL        AD+ V++DGSG F+TI+EA+  A E     
Sbjct: 258 LGTEETEEL-PNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPE----H 317

Query: 266 GSGRFVIYVKGGIYKE-NVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATFAV 325
            S RFVIYVK G Y+E N+ + ++  N+M +GDG  +T++T  K++ D  TTF +ATFA 
Sbjct: 318 SSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAA 377

Query: 326 AGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYRECH 385
            G GFI +DMTFEN AGP KHQAVALR   D ++ YRC+  GYQD LY HSNRQF+REC 
Sbjct: 378 TGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECE 437

Query: 386 IYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAAPD 445
           IYGTVDFIFG+  V+ Q+CNI+ RKPM  QK TITAQ R DPN+N+G  IH   + A PD
Sbjct: 438 IYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPD 497

Query: 446 LKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGD 505
           L+  +G Y TYLGRPWK YSR V M S M   I+P GWL W G F L ++YYGE+MN G 
Sbjct: 498 LEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGL 557

Query: 506 GANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           G+    RV+WPGYHV+TS VEA +FTV  F+ G  W+P+TGV F +GL
Sbjct: 558 GSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

BLAST of CSPI05G19440 vs. TAIR10
Match: AT3G60730.1 (AT3G60730.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 413.7 bits (1062), Expect = 1.8e-115
Identity = 196/317 (61.83%), Postives = 241/317 (76.03%), Query Frame = 1

Query: 223 ADMVVAQDGSGDFRTISEAVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMV 282
           AD VVA+DGS   RTI++A+AA     K S   R +IY+K G+Y E + I++ MKNIM+V
Sbjct: 204 ADFVVARDGSATHRTINQALAAVSRMGK-SRLNRVIIYIKAGVYNEKIEIDRHMKNIMLV 263

Query: 283 GDGMDRTIVTAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNAD 342
           GDGMDRTIVT  +NV DGSTT+ SATF V+G+GF A+D+TFENTAGP KHQAVALR ++D
Sbjct: 264 GDGMDRTIVTNNRNVPDGSTTYGSATFGVSGDGFWARDITFENTAGPHKHQAVALRVSSD 323

Query: 343 RSIFYRCSFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQK 402
            S+FYRCSFKGYQDTL+ HS RQFYR+CHIYGT+DFIFGD   VFQNC+IFVR+PM +Q 
Sbjct: 324 LSLFYRCSFKGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDAAAVFQNCDIFVRRPMDHQG 383

Query: 403 NTITAQARSDPNENSGFVIHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDG 462
           N ITAQ R DP+ NSG  I +S I AAP+ + V+G + +YLGRPWK YSRTV +K+ +D 
Sbjct: 384 NMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYLGRPWKKYSRTVFLKTDIDE 443

Query: 463 LIEPAGWLPWAGDFGLRTVYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFL 522
           LI+P GW  W+G + L T+YYGEFMNTG GA T  RV WPG+HV+    EA  FTV  F+
Sbjct: 444 LIDPRGWREWSGSYALSTLYYGEFMNTGAGAGTGRRVNWPGFHVLRGEEEASPFTVSRFI 503

Query: 523 EGGGWIPATGVPFVNGL 540
           +G  WIP TGVPF  G+
Sbjct: 504 QGDSWIPITGVPFSAGV 519

BLAST of CSPI05G19440 vs. NCBI nr
Match: gi|449464802|ref|XP_004150118.1| (PREDICTED: pectinesterase [Cucumis sativus])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 538/539 (99.81%), Postives = 538/539 (99.81%), Query Frame = 1

Query: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60
           MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF
Sbjct: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60

Query: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120
           RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST
Sbjct: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120

Query: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180
           SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK
Sbjct: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 AIAAAPSTSSSTSCGQPPLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240
           AIAAAPSTSSSTSCGQP LDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE
Sbjct: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240

Query: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300
           AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG
Sbjct: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300

Query: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360
           STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA
Sbjct: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360

Query: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420
           HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV
Sbjct: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420

Query: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480
           IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT
Sbjct: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480

Query: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL
Sbjct: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 539

BLAST of CSPI05G19440 vs. NCBI nr
Match: gi|659072831|ref|XP_008467117.1| (PREDICTED: pectinesterase [Cucumis melo])

HSP 1 Score: 977.2 bits (2525), Expect = 1.1e-281
Identity = 485/539 (89.98%), Postives = 504/539 (93.51%), Query Frame = 1

Query: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60
           MK ++CFLLFSLLVIIHGRSFNNFMSC QTPYPDLCFHYINPNDNI TA IDETYL TRF
Sbjct: 1   MKFVYCFLLFSLLVIIHGRSFNNFMSCFQTPYPDLCFHYINPNDNIPTADIDETYLNTRF 60

Query: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120
           RNSA+QATL QA KV DHLRS MDLGSFDD++RN VALIDCLEFYEDSIA+LNRS  SST
Sbjct: 61  RNSAVQATLNQARKVHDHLRSIMDLGSFDDDNRNKVALIDCLEFYEDSIAKLNRSVFSST 120

Query: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180
           S NSIDH TMLSASLANHQTCLDGFRDF    D+SNNFFLPIQIMSNFSKLVSNSLAITK
Sbjct: 121 SVNSIDHLTMLSASLANHQTCLDGFRDF----DNSNNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 AIAAAPSTSSSTSCGQPPLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240
           A A AP++SSSTSCGQP  DGLCPEWVYDDDKTLL+DMVGKGADMVVAQDGSGDFRTISE
Sbjct: 181 A-AVAPASSSSTSCGQPLPDGLCPEWVYDDDKTLLRDMVGKGADMVVAQDGSGDFRTISE 240

Query: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300
           AVAAAEEARKGSGS RFVIYVKGGIYKENVVI+K MKN+MM+GDGMDRTI+TAMKNVQDG
Sbjct: 241 AVAAAEEARKGSGSERFVIYVKGGIYKENVVIKKSMKNVMMIGDGMDRTIITAMKNVQDG 300

Query: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360
           STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA
Sbjct: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360

Query: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420
           HSNRQFYRECHIYGTVDFIFGDG VVFQNCNIFVRKPM+NQKNTITAQ RSDPNEN+GFV
Sbjct: 361 HSNRQFYRECHIYGTVDFIFGDGAVVFQNCNIFVRKPMMNQKNTITAQGRSDPNENTGFV 420

Query: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480
           IHNSVI AAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSY+DGLIEPAGWLPWAGDFGLRT
Sbjct: 421 IHNSVIKAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYLDGLIEPAGWLPWAGDFGLRT 480

Query: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           +YYGEFMN G GANTKGRV WPGYHVMTSA EAE FTVESFL G GWIP TGVPFVNG+
Sbjct: 481 IYYGEFMNIGGGANTKGRVRWPGYHVMTSATEAEGFTVESFLGGRGWIPTTGVPFVNGV 534

BLAST of CSPI05G19440 vs. NCBI nr
Match: gi|731369412|ref|XP_010654104.1| (PREDICTED: pectinesterase [Vitis vinifera])

HSP 1 Score: 580.5 bits (1495), Expect = 3.1e-162
Identity = 311/539 (57.70%), Postives = 382/539 (70.87%), Query Frame = 1

Query: 3   ILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRN 62
           +LH    + +++ +         SCSQTPYP++C ++I   +   TA IDE      FR+
Sbjct: 5   LLHFLSAYFVVLYVRVVDGAPISSCSQTPYPEVCNYFIG--NYKPTAGIDEIQF--PFRD 64

Query: 63  SAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSA 122
             +  T+ QA ++   L S MDL S D+  R  +A  DCLE YE++I  +NRS  S + A
Sbjct: 65  RVLGVTMNQAKRLH-LLVSAMDLSSSDE--RTKLAWADCLELYENTIDLVNRSINSISPA 124

Query: 123 NSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAI 182
              D  T LSA++AN QTCLDGF DF    D   +F       SNFSKL+SNSLAI KA 
Sbjct: 125 VMFDSQTWLSAAIANQQTCLDGFIDFNPSSDQFQSFPSMSISTSNFSKLLSNSLAINKAA 184

Query: 183 AAAPSTSSSTSCG-QPPLDGLCPEWVYDDDKTLLQDM-VGKGADMVVAQDGSGDFRTISE 242
            +A S  S+   G +  L    P WV   D+ LLQ       AD+VVA DGSG+++TISE
Sbjct: 185 VSATSILSNNQAGGRRLLSNGFPTWVSAADRKLLQSSGAASRADIVVAHDGSGNYKTISE 244

Query: 243 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 302
           AVAA+ + R  SG+ RFVIYVK G+Y+ENV I+++MKNIM++GDG D TIVT  KNVQDG
Sbjct: 245 AVAASVKLR--SGTKRFVIYVKAGVYRENVEIKRKMKNIMIIGDGKDATIVTGNKNVQDG 304

Query: 303 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 362
           STTF+SATFAV+G GFIA+DMTFENTAGPEKHQAVALRS++D S+FY CSFKGYQDTLY 
Sbjct: 305 STTFRSATFAVSGHGFIARDMTFENTAGPEKHQAVALRSSSDGSVFYGCSFKGYQDTLYV 364

Query: 363 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 422
           H+ RQFYR C +YGTVDFIFGD V V QNCNI+VR+PM NQ N ITAQ RSD NEN+G  
Sbjct: 365 HTQRQFYRSCDVYGTVDFIFGDAVAVLQNCNIYVRRPMSNQANVITAQGRSDQNENTGIS 424

Query: 423 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 482
           IHNS + AAPDL+PVQ  + TYLGRPW+ YSRTV MK+ +DGLI P GW PW GDFGL T
Sbjct: 425 IHNSRVMAAPDLRPVQSRFKTYLGRPWRKYSRTVFMKTSLDGLIHPEGWSPWKGDFGLST 484

Query: 483 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           +YYGE+MNTG GA+T+GRV+W GYHV+TSA EA++FTV  FL G  WIP TGVP+ +GL
Sbjct: 485 LYYGEYMNTGSGASTRGRVKWRGYHVITSAAEADKFTVGRFLVGDSWIPTTGVPYASGL 534

BLAST of CSPI05G19440 vs. NCBI nr
Match: gi|1009154925|ref|XP_015895436.1| (PREDICTED: pectinesterase [Ziziphus jujuba])

HSP 1 Score: 575.5 bits (1482), Expect = 9.9e-161
Identity = 306/548 (55.84%), Postives = 392/548 (71.53%), Query Frame = 1

Query: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYI---NPN--DNIRTAHI---- 60
           + I + + L   L ++HG        CSQTPYP +C H +   NP   +N   A++    
Sbjct: 9   LAIFYAYFLAFPLHVVHGSIVT---PCSQTPYPQVCEHVLCSQNPKVCENFTNANLLSNL 68

Query: 61  DETYLITRFRNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAE 120
           D+T     F + A++ T+ QA+     L S+MDL SF  N+++ +A  DCLE Y+D+I +
Sbjct: 69  DQTGFT--FLDLALKTTMEQAVDAHQ-LVSSMDLNSF--NEQSKLAWNDCLELYKDTIDQ 128

Query: 121 LNRSTLSSTSANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKL 180
           LNRS  SS   N +D +T LSAS+ANHQTC +GF DF      S+  F P  ++SNFSK+
Sbjct: 129 LNRSMSSS---NPLDANTWLSASIANHQTCQNGFIDFKL---SSDLDFFP-SMLSNFSKM 188

Query: 181 VSNSLAITKAIAAAPSTSSSTSCGQPPLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDG 240
           +SNSLAI KA    P+       G+  L  + P WV   D+ LLQ       D++VAQDG
Sbjct: 189 LSNSLAIHKASTFVPTKEIH---GRRLLSNVFPRWVSIADRKLLQSEQAPKGDIIVAQDG 248

Query: 241 SGDFRTISEAVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIV 300
           +G+++TI+EAV  A  A+ G G+ R VIYVK G+YKENV I+K +KN+M VGDG+D TIV
Sbjct: 249 TGNYKTITEAVTQA--AKLGGGNKRIVIYVKAGVYKENVDIKKTIKNLMFVGDGIDATIV 308

Query: 301 TAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSF 360
           T  ++ + GSTTF+SATFAV+G+GF+A+DMTFENTAGP+ HQAVA RS +D S+FYRCSF
Sbjct: 309 TGGRSNEGGSTTFRSATFAVSGDGFMARDMTFENTAGPQDHQAVAFRSGSDHSVFYRCSF 368

Query: 361 KGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARS 420
           +GYQDTLY +S RQFYR+C IYGTVDFIFGD   +FQNCNI+VRKPM NQKN++TAQ R+
Sbjct: 369 RGYQDTLYVYSQRQFYRDCDIYGTVDFIFGDAAAIFQNCNIYVRKPMSNQKNSVTAQGRT 428

Query: 421 DPNENSGFVIHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLP 480
           DPNEN+G +IHNS I AA DL+PVQG + TYLGRPW+ YSRTVIMK+ +DGL+ PAGW P
Sbjct: 429 DPNENTGIIIHNSRILAAADLRPVQGSFETYLGRPWQKYSRTVIMKTTIDGLVSPAGWEP 488

Query: 481 WAGDFGLRTVYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPAT 540
           W+G F L T+YYGE+MN G GA T GRV+WPG+HV+TS VEA +F+VESFL GG WIPAT
Sbjct: 489 WSGSFALSTLYYGEYMNVGPGAGTGGRVKWPGFHVITSPVEASKFSVESFLAGGSWIPAT 536

BLAST of CSPI05G19440 vs. NCBI nr
Match: gi|703119879|ref|XP_010101977.1| (putative pectinesterase/pectinesterase inhibitor 6 [Morus notabilis])

HSP 1 Score: 573.9 bits (1478), Expect = 2.9e-160
Identity = 306/530 (57.74%), Postives = 383/530 (72.26%), Query Frame = 1

Query: 13  LVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQA 72
           + ++HG   +   +C+QTPYP +C H++N N     + +D+   ++ F + A++AT+ QA
Sbjct: 1   MYVVHGTK-STISTCNQTPYPQVCHHFLNTN---HLSTLDDKTTVS-FHDLALKATMKQA 60

Query: 73  MKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSIDHSTMLS 132
           ++    L STMD  SF   +R   A  DCLE Y+D++  LNRS ++S + N +D  T LS
Sbjct: 61  VEAH-RLVSTMDSDSF--GERGKSAWQDCLELYKDTVDLLNRS-VNSNNKNPLDTHTWLS 120

Query: 133 ASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAAAPSTSSST 192
           ASLAN  TC +GF DF      S+    P  ++SNFSKL+SNSLAI KA   + +TS S 
Sbjct: 121 ASLANQHTCRNGFTDFQI---PSHYDSFP-SMLSNFSKLLSNSLAINKATIPSTATSFSK 180

Query: 193 SCGQPPL--DGLCPEWVYDDDKTLLQDM-VGKGADMVVAQDGSGDFRTISEAVAAAEEAR 252
                 L  DG  PEWV   D+ LLQ       AD+VVAQDGSG F+TISEAV AA+   
Sbjct: 181 QVRSRRLLSDGF-PEWVSAADRKLLQSGGAAAKADIVVAQDGSGGFKTISEAVEAAKNL- 240

Query: 253 KGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTFQSATF 312
            G GS R V+YVK G+YKENV I+  +KN+M VGDG+D TIVT  +N  DGSTTFQSATF
Sbjct: 241 -GGGSKRIVVYVKAGVYKENVEIKNDIKNLMFVGDGIDATIVTGNRNNVDGSTTFQSATF 300

Query: 313 AVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYRE 372
            V+G+GFIA+DMTFENTAGP KHQAVALRS +D S+F+RCSFKGYQDTLY +S RQFYR+
Sbjct: 301 GVSGDGFIAQDMTFENTAGPAKHQAVALRSGSDHSVFHRCSFKGYQDTLYVYSQRQFYRD 360

Query: 373 CHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNSVIGAA 432
           C++YGTVDFI GD V + QNCNI VRKPM +Q+NT+T+QAR+DPNEN+G +IHNS I A+
Sbjct: 361 CNVYGTVDFICGDAVAILQNCNILVRKPMSDQRNTVTSQARTDPNENTGIIIHNSRITAS 420

Query: 433 PDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYGEFMNT 492
            DL+PVQG + TYLGRPW+ YSRTVIMKS +DGLI+P GWLPW+G+F L T+YY EFMN+
Sbjct: 421 SDLRPVQGSFETYLGRPWQKYSRTVIMKSSLDGLIDPKGWLPWSGNFALSTLYYAEFMNS 480

Query: 493 GDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 540
           G GA T GRV WPGYHV+TSA EA +FTV +FL G  WIPATGVPF  GL
Sbjct: 481 GAGAATGGRVSWPGYHVLTSAAEAGKFTVGNFLGGDSWIPATGVPFAAGL 514