Lsi09G006730 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi09G006730 vs. Swiss-Prot
Match: PME6_ARATH (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana GN=PME6 PE=2 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 2.0e-145
Identity = 283/560 (50.54%), Postives = 376/560 (67.14%), Query Frame = 1

Query: 2   KMLHSLLLFSLLVIIHGKSFNNFM--SCSQTPYPDLCFHYINPNDDVSTVNIDETSLITR 61
           K L++  + +++ ++     N     SC QTPYP +C H+++ N  + T++ D+T   T 
Sbjct: 11  KSLYTKCIITIIYVVSISHLNAHFITSCKQTPYPSVCDHHMS-NSPLKTLD-DQTDGFT- 70

Query: 62  FENLAIRATLNQAMVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINP 121
           F +L + +T++QA+ +H  + S     S      +  AL DCLELYED+I +LNHS  + 
Sbjct: 71  FHDLVVSSTMDQAVQLHRLVSSLKQHHSLHKHATS--ALFDCLELYEDTIDQLNHSRRSY 130

Query: 122 MSVNLI-DHSTLLSASLANYQTCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSNSLAI 181
              +   D  T LSA++AN  TC+ GF+DF  +   S + +FP Q   N +K +SNSLA+
Sbjct: 131 GQYSSPHDRQTSLSAAIANQDTCRNGFRDFKLT--SSYSKYFPVQFHRNLTKSISNSLAV 190

Query: 182 TKATAVAATT----------------SSTSGG--RRLLGGS---FPEWVYDDDRMLLQGA 241
           TKA A A                   SS  GG  RRLL  S   FP W    DR LL+ +
Sbjct: 191 TKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDS 250

Query: 242 VGN-RVDMVVAQDGSGDFRTISEAVAAVEEARKGSERFVIYVKGGIYKENVVIKKSMKNI 301
               + D+VVA+DGSG + +I +AV A  +  + ++R VIYVK G+Y+ENVVIKKS+KN+
Sbjct: 251 KTTAKADLVVAKDGSGHYTSIQQAVNAAAKLPRRNQRLVIYVKAGVYRENVVIKKSIKNV 310

Query: 302 MMIGDGMDRTIVTAMKNVQDGSTTFRSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRS 361
           M+IGDG+D TIVT  +NVQDG+TTFRSATFAV+G GFIA+ +TFENTAGPEKHQAVALRS
Sbjct: 311 MVIGDGIDSTIVTGNRNVQDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRS 370

Query: 362 NADRSIFYRCSFNGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVAVFQNCNIFVRKPMM 421
           ++D S+FY CSF GYQDTLY HS+RQF R C+IYGTVDFIFGD  A+ QNCNI+ RKPM 
Sbjct: 371 SSDFSVFYACSFKGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYARKPMS 430

Query: 422 NQKNTITAQARSDPNENSGFVIHNSVIGAAPDLKPVEGLYGTYLGRPWKAYSRTVVMRSF 481
            QKNTITAQ+R +P+E +GFVI +S +  A +         TYLGRPW+++SRTV M+  
Sbjct: 431 GQKNTITAQSRKEPDETTGFVIQSSTVATASE---------TYLGRPWRSHSRTVFMKCN 490

Query: 482 LDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGGGANTKGRVQWPGYHVLTSTAEAERFTVE 537
           L  L+ PAGWLPW+G F L T+YYGE+ NTG GA+  GRV+WPGYHV+ +  EAE+FTVE
Sbjct: 491 LGALVSPAGWLPWSGSFALSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVE 550

BLAST of Lsi09G006730 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 7.8e-134
Identity = 258/532 (48.50%), Postives = 343/532 (64.47%), Query Frame = 1

Query: 6   SLLLFSLLVIIHGKSFNNFMS-CSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFENLA 65
           SL+LFSL     G S     S C +TP P  C +++    DV+++  D     T F  ++
Sbjct: 10  SLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQD-----TDFYKIS 69

Query: 66  IRATLNQAMVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINPMSVNL 125
           ++  L +A         T  L S   +++ K A  DC ELYE ++++LN ++ +      
Sbjct: 70  LQLALERATTAQSR---TYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTK 129

Query: 126 IDHSTLLSASLANYQTCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSNSLAITKATAV 185
           +D  T LS +L N +TC+   +D G        +  P  + +N +KL+SN+L++ K   V
Sbjct: 130 VDKQTWLSTALTNLETCRASLEDLGVP-----EYVLPL-LSNNVTKLISNTLSLNK---V 189

Query: 186 AATTSSTSGGRRLLGGSFPEWVYDDDRMLLQGAVGNRVDMVVAQDGSGDFRTISEAVAAV 245
                S   G       FP WV   DR LLQ     R ++VVAQDGSG+ +TI EAVAA 
Sbjct: 190 PYNEPSYKDG-------FPTWVKPGDRKLLQTTP--RANIVVAQDGSGNVKTIQEAVAAA 249

Query: 246 EEARKGSERFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGSTTFRSA 305
             +R G  R+VIY+K G Y EN+ +K  +KNIM +GDG+ +TI+T  K+V  G+TTF+SA
Sbjct: 250 --SRAGGSRYVIYIKAGTYNENIEVK--LKNIMFVGDGIGKTIITGSKSVGGGATTFKSA 309

Query: 306 TFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYAHSNRQFY 365
           T AV G+ FIA+D+T  NTAGP  HQAVALRS +D S+FYRCSF GYQDTLY HS RQFY
Sbjct: 310 TVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFY 369

Query: 366 RECHIYGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFVIHNSVIG 425
           REC IYGTVDFIFG+   V QNCNIF RKP  N+ NT+TAQ R+DPN+++G +IHN  + 
Sbjct: 370 RECDIYGTVDFIFGNAAVVLQNCNIFARKPP-NRTNTLTAQGRTDPNQSTGIIIHNCRVT 429

Query: 426 AAPDLKPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLRTVYYGEFM 485
           AA DLKPV+    T+LGRPWK YSRTV +++FLD LI PAGW+ W+GDF L T+YY E+M
Sbjct: 430 AASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYM 489

Query: 486 NTGGGANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
           NTG G++T  RV+W GYHVLTS ++  +FTV +F+ G  WLPAT VPF +GL
Sbjct: 490 NTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of Lsi09G006730 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 448.7 bits (1153), Expect = 8.7e-125
Identity = 242/510 (47.45%), Postives = 330/510 (64.71%), Query Frame = 1

Query: 27  CSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFENLAIRATLNQAMVVHDHLRSTMDLD 86
           CSQTP P  C +++  N +   +  +     + F  ++++  L++A++   H      L 
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSE-----SEFLKISMKLVLDRAILAKTH---AFTLG 93

Query: 87  SFDNDDKTKFALIDCLELYEDSIIELNHSTINPMSVNLIDHSTLLSASLANYQTCQEGFQ 146
               D + K A  DC++LY+ ++ ++N +    +  + +D  T LS +L N  TC+ GF 
Sbjct: 94  PKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFL 153

Query: 147 DFGFSFDDSSNFFFPFQILSNFSKLVSNSLAITKATAVAATTSSTSGGRRLLGGSFPEWV 206
           + G +     +   P  + +N S L+ N+LAI K      T     G        FP WV
Sbjct: 154 ELGVT-----DIVLPL-MSNNVSNLLCNTLAINKVP-FNYTPPEKDG--------FPSWV 213

Query: 207 YDDDRMLLQGAVGNRVDMVVAQDGSGDFRTISEAVAAVEEARKGSERFVIYVKGGIYKEN 266
              DR LLQ +   + + VVA+DGSG+F+TI EA+    +A  GS RFVIYVK G+Y EN
Sbjct: 214 KPGDRKLLQSSTP-KDNAVVAKDGSGNFKTIKEAI----DAASGSGRFVIYVKQGVYSEN 273

Query: 267 VVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGSTTFRSATFAVAGEGFIAKDMTFENTAGP 326
           + I+K  KN+M+ GDG+ +TI+T  K+V  G+TTF SAT A  G+GFIA+ +TF NTAG 
Sbjct: 274 LEIRK--KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGA 333

Query: 327 EKHQAVALRSNADRSIFYRCSFNGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVAVFQN 386
              QAVALRS +D S+FY+CSF  YQDTLY HSNRQFYR+C +YGTVDFIFG+  AV QN
Sbjct: 334 SNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQN 393

Query: 387 CNIFVRKPMMNQKNTITAQARSDPNENSGFVIHNSVIGAAPDLKPVEGLYGTYLGRPWKA 446
           CNIF R+P  ++ NTITAQ RSDPN+N+G +IHNS + AA DL+PV G   TYLGRPW+ 
Sbjct: 394 CNIFARRPR-SKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQ 453

Query: 447 YSRTVVMRSFLDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGGGANTKGRVQWPGYHVLTS 506
           YSRTV M++ LD LI+P GWL W G+F L+T++Y EF NTG GA+T GRV WPG+ VL S
Sbjct: 454 YSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGS 511

Query: 507 TAEAERFTVESFLEGGDWLPATGVPFANGL 537
            +EA +FTV +FL GG W+P++ VPF +GL
Sbjct: 514 ASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Lsi09G006730 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 2.6e-121
Identity = 249/529 (47.07%), Postives = 325/529 (61.44%), Query Frame = 1

Query: 26  SCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFENLAIRATLNQAMVVHDHLRSTMDL 85
           SCS T YPDLCF  I    + S     +  +I    N+   A      V H++      L
Sbjct: 72  SCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTA------VEHNYFGIQKLL 131

Query: 86  DSFDNDDKTKFALIDCLELYEDSIIELNHSTIN----PMSVNLIDHS----TLLSASLAN 145
              +   + K AL DCLE  ++++ EL+ +  +    P   +L  H+    TL+SA++ N
Sbjct: 132 KRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 191

Query: 146 YQTCQEGFQDFGFSFDDSSNFFFPFQILSN----FSKLVSNSLAITK-ATAVAATTSSTS 205
             TC +GF     S DD++        LS+      K+ SN+LA+ K  T        TS
Sbjct: 192 QGTCLDGF-----SHDDANKHVR--DALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTS 251

Query: 206 GGRRLLGGS-----FPEWVYDDDRMLLQGAVGNRVDMVVAQDGSGDFRTISEAVAAVEEA 265
             R+L   +     +P W+   DR LLQ +     + VVA DGSG+F+T++ AVAA  + 
Sbjct: 252 NNRKLTEETSTVDGWPAWLSPGDRRLLQSS-SVTPNAVVAADGSGNFKTVAAAVAAAPQG 311

Query: 266 RKGSERFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGSTTFRSATFA 325
             G++R++I +K G+Y+ENV + K  KNIM IGDG  RTI+T  +NV DGSTTF+SAT A
Sbjct: 312 --GTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAA 371

Query: 326 VAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYAHSNRQFYREC 385
           V GEGF+A+D+TF+NTAGP KHQAVALR  AD S FY C    YQDTLY HSNRQF+  C
Sbjct: 372 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 431

Query: 386 HIYGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFVIHNSVIGAAP 445
            I GTVDFIFG+  AV QNC+I  RKP   QKN +TAQ R+DPN+N+G VI  S IGA  
Sbjct: 432 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATS 491

Query: 446 DLKPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLRTVYYGEFMNTG 505
           DLKPV+G + TYLGRPWK YSRTV+M+S +  LI PAGW  W G+F L T++YGE  N+G
Sbjct: 492 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSG 551

Query: 506 GGANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
            GA T GRV+W G+ V+TS  EA+ FT  SF+ G  WL +TG PF+ GL
Sbjct: 552 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Lsi09G006730 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 3.4e-121
Identity = 247/529 (46.69%), Postives = 326/529 (61.63%), Query Frame = 1

Query: 26  SCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFENLAIRATLNQAMVVHDHLRSTMDL 85
           SCS T YPDLCF  I    + S     +  +I    N+   A      V H++      L
Sbjct: 72  SCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTA------VEHNYFGIQKLL 131

Query: 86  DSFDNDDKTKFALIDCLELYEDSIIELNHSTIN----PMSVNLIDHS----TLLSASLAN 145
              +   + K AL DCLE  ++++ EL+ +  +    P   +L  H+    TL+SA++ N
Sbjct: 132 KRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 191

Query: 146 YQTCQEGFQDFGFSFDDSSNFFFPFQILSN----FSKLVSNSLAITK-ATAVAATTSSTS 205
             TC +GF     S DD++        LS+      K+ SN+LA+ K  T        TS
Sbjct: 192 QGTCLDGF-----SHDDANKHVR--DALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTS 251

Query: 206 GGRRLLGGS-----FPEWVYDDDRMLLQGAVGNRVDMVVAQDGSGDFRTISEAVAAVEEA 265
             R+L+  +     +P W+   DR LLQ +     ++VVA DGSG+F+T++ +VAA  + 
Sbjct: 252 NNRKLIEETSTVDGWPAWLSTGDRRLLQSS-SVTPNVVVAADGSGNFKTVAASVAAAPQG 311

Query: 266 RKGSERFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGSTTFRSATFA 325
             G++R++I +K G+Y+ENV + K  KNIM IGDG  RTI+T  +NV DGSTTF+SAT A
Sbjct: 312 --GTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVA 371

Query: 326 VAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYAHSNRQFYREC 385
           V GEGF+A+D+TF+NTAGP KHQAVALR  AD S FY C    YQDTLY HSNRQF+  C
Sbjct: 372 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 431

Query: 386 HIYGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFVIHNSVIGAAP 445
            I GTVDFIFG+  AV QNC+I  RKP   QKN +TAQ R+DPN+N+G VI  S IGA  
Sbjct: 432 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATS 491

Query: 446 DLKPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLRTVYYGEFMNTG 505
           DLKPV+G + TYLGRPWK YSRTV+M+S +  +I PAGW  W G+F L T++YGE  N G
Sbjct: 492 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAG 551

Query: 506 GGANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
            GA T GRV+W G+ V+TS  EA+ FT  SF+ G  WL +TG PF+ GL
Sbjct: 552 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Lsi09G006730 vs. TrEMBL
Match: A0A0A0KUU2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G568300 PE=4 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 8.1e-263
Identity = 454/539 (84.23%), Postives = 487/539 (90.35%), Query Frame = 1

Query: 1   MKMLHSLLLFSLLVIIHGKSFNNFMSCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRF 60
           MK+LH  LLFSLLVIIHG+SFNNFMSCSQTPYPDLCFHYINPND++ T +IDET LITRF
Sbjct: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60

Query: 61  ENLAIRATLNQAMVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINPM 120
            N AI+ATL QAM V DHLRSTMDL SFD++D+   ALIDCLE YEDSI ELN ST++  
Sbjct: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120

Query: 121 SVNLIDHSTLLSASLANYQTCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSNSLAITK 180
           S N IDHST+LSASLAN+QTC +GF+DFGF  DDS+NFF P QI+SNFSKLVSNSLAITK
Sbjct: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 ATAVA-ATTSSTSGGRRLLGGSFPEWVYDDDRMLLQGAVGNRVDMVVAQDGSGDFRTISE 240
           A A A +T+SSTS G+ LL G  PEWVYDDD+ LLQ  VG   DMVVAQDGSGDFRTISE
Sbjct: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240

Query: 241 AVAAVEEARKGSE--RFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDG 300
           AVAA EEARKGS   RFVIYVKGGIYKENVVI+K MKNIMM+GDGMDRTIVTAMKNVQDG
Sbjct: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300

Query: 301 STTFRSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYA 360
           STTF+SATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSF GYQDTLYA
Sbjct: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360

Query: 361 HSNRQFYRECHIYGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFV 420
           HSNRQFYRECHIYGTVDFIFGDGV VFQNCNIFVRKPM+NQKNTITAQARSDPNENSGFV
Sbjct: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420

Query: 421 IHNSVIGAAPDLKPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLRT 480
           IHNSVIGAAPDLKPV+GLYGTYLGRPWKAYSRTV+M+S++DGLIEPAGWLPWAGDFGLRT
Sbjct: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480

Query: 481 VYYGEFMNTGGGANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
           VYYGEFMNTG GANTKGRV+WPGYHV+TS  EAERFTVESFLEGG W+PATGVPF NGL
Sbjct: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 539

BLAST of Lsi09G006730 vs. TrEMBL
Match: F6HFF6_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_01s0011g05380 PE=4 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 2.3e-169
Identity = 318/538 (59.11%), Postives = 392/538 (72.86%), Query Frame = 1

Query: 3   MLHSLLLFSLLVIIHGKSFNNFMSCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFEN 62
           +LH L  + +++ +         SCSQTPYP++C ++I   +   T  IDE      F +
Sbjct: 5   LLHFLSAYFVVLYVRVVDGAPISSCSQTPYPEVCNYFIG--NYKPTAGIDEIQF--PFRD 64

Query: 63  LAIRATLNQAMVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINPMSV 122
             +  T+NQA  +H  L S MDL S  +D++TK A  DCLELYE++I  +N S  +    
Sbjct: 65  RVLGVTMNQAKRLH-LLVSAMDLSS--SDERTKLAWADCLELYENTIDLVNRSINSISPA 124

Query: 123 NLIDHSTLLSASLANYQTCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSNSLAITKAT 182
            + D  T LSA++AN QTC +GF DF  S D   +F       SNFSKL+SNSLAI KA 
Sbjct: 125 VMFDSQTWLSAAIANQQTCLDGFIDFNPSSDQFQSFPSMSISTSNFSKLLSNSLAINKA- 184

Query: 183 AVAATT---SSTSGGRRLLGGSFPEWVYDDDRMLLQGA-VGNRVDMVVAQDGSGDFRTIS 242
           AV+AT+   ++ +GGRRLL   FP WV   DR LLQ +   +R D+VVA DGSG+++TIS
Sbjct: 185 AVSATSILSNNQAGGRRLLSNGFPTWVSAADRKLLQSSGAASRADIVVAHDGSGNYKTIS 244

Query: 243 EAVAAVEEARKGSERFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGS 302
           EAVAA  + R G++RFVIYVK G+Y+ENV IK+ MKNIM+IGDG D TIVT  KNVQDGS
Sbjct: 245 EAVAASVKLRSGTKRFVIYVKAGVYRENVEIKRKMKNIMIIGDGKDATIVTGNKNVQDGS 304

Query: 303 TTFRSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYAH 362
           TTFRSATFAV+G GFIA+DMTFENTAGPEKHQAVALRS++D S+FY CSF GYQDTLY H
Sbjct: 305 TTFRSATFAVSGHGFIARDMTFENTAGPEKHQAVALRSSSDGSVFYGCSFKGYQDTLYVH 364

Query: 363 SNRQFYRECHIYGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFVI 422
           + RQFYR C +YGTVDFIFGD VAV QNCNI+VR+PM NQ N ITAQ RSD NEN+G  I
Sbjct: 365 TQRQFYRSCDVYGTVDFIFGDAVAVLQNCNIYVRRPMSNQANVITAQGRSDQNENTGISI 424

Query: 423 HNSVIGAAPDLKPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLRTV 482
           HNS + AAPDL+PV+  + TYLGRPW+ YSRTV M++ LDGLI P GW PW GDFGL T+
Sbjct: 425 HNSRVMAAPDLRPVQSRFKTYLGRPWRKYSRTVFMKTSLDGLIHPEGWSPWKGDFGLSTL 484

Query: 483 YYGEFMNTGGGANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
           YYGE+MNTG GA+T+GRV+W GYHV+TS AEA++FTV  FL G  W+P TGVP+A+GL
Sbjct: 485 YYGEYMNTGSGASTRGRVKWRGYHVITSAAEADKFTVGRFLVGDSWIPTTGVPYASGL 534

BLAST of Lsi09G006730 vs. TrEMBL
Match: W9S5Z7_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_011995 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 1.2e-168
Identity = 313/527 (59.39%), Postives = 392/527 (74.38%), Query Frame = 1

Query: 13  LVIIHGKSFNNFMSCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFENLAIRATLNQA 72
           + ++HG   +   +C+QTPYP +C H++N N  +ST++ D+T++   F +LA++AT+ QA
Sbjct: 1   MYVVHGTK-STISTCNQTPYPQVCHHFLNTNH-LSTLD-DKTTV--SFHDLALKATMKQA 60

Query: 73  MVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINPMSVNLIDHSTLLS 132
           +  H  L STMD DSF   ++ K A  DCLELY+D++  LN S +N  + N +D  T LS
Sbjct: 61  VEAH-RLVSTMDSDSFG--ERGKSAWQDCLELYKDTVDLLNRS-VNSNNKNPLDTHTWLS 120

Query: 133 ASLANYQTCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSNSLAITKATAVAATTSSTS 192
           ASLAN  TC+ GF DF       S   FP  +LSNFSKL+SNSLAI KAT  +  TS + 
Sbjct: 121 ASLANQHTCRNGFTDFQIPSHYDS---FP-SMLSNFSKLLSNSLAINKATIPSTATSFSK 180

Query: 193 G--GRRLLGGSFPEWVYDDDRMLLQ-GAVGNRVDMVVAQDGSGDFRTISEAVAAVEEARK 252
               RRLL   FPEWV   DR LLQ G    + D+VVAQDGSG F+TISEAV A +    
Sbjct: 181 QVRSRRLLSDGFPEWVSAADRKLLQSGGAAAKADIVVAQDGSGGFKTISEAVEAAKNLGG 240

Query: 253 GSERFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGSTTFRSATFAVA 312
           GS+R V+YVK G+YKENV IK  +KN+M +GDG+D TIVT  +N  DGSTTF+SATF V+
Sbjct: 241 GSKRIVVYVKAGVYKENVEIKNDIKNLMFVGDGIDATIVTGNRNNVDGSTTFQSATFGVS 300

Query: 313 GEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYAHSNRQFYRECHI 372
           G+GFIA+DMTFENTAGP KHQAVALRS +D S+F+RCSF GYQDTLY +S RQFYR+C++
Sbjct: 301 GDGFIAQDMTFENTAGPAKHQAVALRSGSDHSVFHRCSFKGYQDTLYVYSQRQFYRDCNV 360

Query: 373 YGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFVIHNSVIGAAPDL 432
           YGTVDFI GD VA+ QNCNI VRKPM +Q+NT+T+QAR+DPNEN+G +IHNS I A+ DL
Sbjct: 361 YGTVDFICGDAVAILQNCNILVRKPMSDQRNTVTSQARTDPNENTGIIIHNSRITASSDL 420

Query: 433 KPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGGG 492
           +PV+G + TYLGRPW+ YSRTV+M+S LDGLI+P GWLPW+G+F L T+YY EFMN+G G
Sbjct: 421 RPVQGSFETYLGRPWQKYSRTVIMKSSLDGLIDPKGWLPWSGNFALSTLYYAEFMNSGAG 480

Query: 493 ANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
           A T GRV WPGYHVLTS AEA +FTV +FL G  W+PATGVPFA GL
Sbjct: 481 AATGGRVSWPGYHVLTSAAEAGKFTVGNFLGGDSWIPATGVPFAAGL 514

BLAST of Lsi09G006730 vs. TrEMBL
Match: B9HW40_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0010s11960g PE=4 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 4.6e-165
Identity = 305/542 (56.27%), Postives = 391/542 (72.14%), Query Frame = 1

Query: 4   LHSLLLFSLLVIIHGKSFNNFMSCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFENL 63
           L++  LF  L ++HG S     SC QTP+P+ C ++I+        NI +T  +    + 
Sbjct: 11  LNAYFLFYSLALVHGDSVT---SCDQTPFPEACNYFID-------TNISKTPPLFALRDQ 70

Query: 64  AIRATLNQAMVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINPMSVN 123
           ++  T+N+A+  H  + S+M+L SF+   + K A  DCL+LYED++  +N S     S N
Sbjct: 71  SLSITMNKAIEAHQMV-SSMELSSFNQ--QAKLAWDDCLKLYEDTVDHVNRSM---SSNN 130

Query: 124 LIDHSTLLSASLANYQTCQEGFQDFGF-SFDDSSNFFFPFQILSNFSKLVSNSLAITKA- 183
           L D  T LSA++AN +TC+ GF DF   S+ +S        +L NF+KL+SN+L++ KA 
Sbjct: 131 LADSQTWLSAAIANQRTCENGFIDFNIVSYLESLP-----NMLRNFTKLLSNTLSLNKAI 190

Query: 184 ---TAVAATTSSTSGGRRLLGGSFPEWVYDDDRMLLQ--GAVGNRVDMVVAQDGSGDFRT 243
              T +   T    G RRLL   FP WV   DR LLQ  G    + D+VVAQDGSGD++T
Sbjct: 191 ISSTPILLDTKQDGGRRRLLVDGFPSWVPASDRKLLQSNGRAAPKADIVVAQDGSGDYKT 250

Query: 244 ISEAVAAVEEARKG--SERFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNV 303
           ISEAVAA  + R G  S+RFVIYVKGG+YKENV IKKSMKN+M +GDG+D T++T+ KN 
Sbjct: 251 ISEAVAASAKLRSGTKSKRFVIYVKGGVYKENVEIKKSMKNLMFVGDGIDATVITSNKNT 310

Query: 304 QDGSTTFRSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDT 363
           QDG+TTFRSAT  V+G+GFIA+D+TFENTAGP+KHQAVALRS +D S+FY CSF GYQDT
Sbjct: 311 QDGTTTFRSATVGVSGKGFIARDITFENTAGPQKHQAVALRSGSDFSVFYSCSFKGYQDT 370

Query: 364 LYAHSNRQFYRECHIYGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENS 423
           LY HS RQFYR+C IYGTVDFIFGD VAV QNCNI+VR+PM  Q NT+TAQ R+DP+EN+
Sbjct: 371 LYVHSQRQFYRDCDIYGTVDFIFGDAVAVLQNCNIYVRRPMSKQTNTVTAQGRTDPDENT 430

Query: 424 GFVIHNSVIGAAPDLKPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFG 483
           G VIHNS + AAPDL+PV+G + +YLGRPWK YSRTV ++S +DGLI+PAGWLPW GDF 
Sbjct: 431 GIVIHNSRVMAAPDLRPVQGSFKSYLGRPWKKYSRTVFLKSNIDGLIDPAGWLPWKGDFA 490

Query: 484 LRTVYYGEFMNTGGGANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFAN 537
           L T+YYGE+M+TG GA+TKGRV+WPGYH +TS  EA +FTVE+FL G  W+ A GVPF +
Sbjct: 491 LSTLYYGEYMSTGSGASTKGRVKWPGYHTITSPLEAGKFTVENFLAGNSWISAAGVPFES 531

BLAST of Lsi09G006730 vs. TrEMBL
Match: B9SP63_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_0628790 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 1.1e-163
Identity = 303/530 (57.17%), Postives = 380/530 (71.70%), Query Frame = 1

Query: 9   LFSLLVIIHGKSFNNFMSCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFENLAIRAT 68
           LF  L +IHG       SC++TPYP++C ++I  N   +     +T     F + ++  T
Sbjct: 14  LFYSLELIHGSKL--ITSCARTPYPEVCNYFIETNLLQTQY---QTGTTFSFRDQSLLVT 73

Query: 69  LNQAMVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINPMSVNLIDHS 128
           +NQA+  H  + S+M+  SFD   K K A  DC+ELYED++  LN S  + +    ID  
Sbjct: 74  MNQAIKAHQMV-SSMNFKSFDK--KAKLAWDDCMELYEDTVDHLNRSLSSTIP---IDSQ 133

Query: 129 TLLSASLANYQTCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSNSLAITKATAVAATT 188
           T LSA++AN QTCQ GF D   S+DD      P  +LSN S L+SNSLA+ K + V   T
Sbjct: 134 TWLSAAIANQQTCQNGFIDLNLSYDDHLESM-PI-MLSNLSMLLSNSLAVNKVS-VPHNT 193

Query: 189 SSTSGGRRLLGGSFPEWVYDDDRMLLQGAVG--NRVDMVVAQDGSGDFRTISEAVAAVEE 248
              +G R L+   FP WV   DR LLQ + G   + D+VVAQDGSG+++TI+EAVAA  +
Sbjct: 194 KQVNGRRLLIFDGFPSWVSATDRRLLQSSSGVAPKADIVVAQDGSGNYKTITEAVAAAVK 253

Query: 249 ARKGSERFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGSTTFRSATF 308
            R GS+R VIYVK GIYKEN+ IKKSMKN+M +GDG+D TIVT  KN +DGSTTFRSATF
Sbjct: 254 QRSGSKRLVIYVKKGIYKENIEIKKSMKNLMFVGDGIDATIVTGSKNAKDGSTTFRSATF 313

Query: 309 AVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYAHSNRQFYRE 368
           AV+G+GFIAK MTFENTAGP+KHQAVALRS +D S+FY CSF GYQDTLY +S RQFYR+
Sbjct: 314 AVSGQGFIAKGMTFENTAGPQKHQAVALRSGSDFSVFYGCSFKGYQDTLYVYSQRQFYRD 373

Query: 369 CHIYGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFVIHNSVIGAA 428
           C IYGT+DFIFGD VAV QNCNI+VR+PM  QKNT+TAQ R DPNEN+G VIHNS + A 
Sbjct: 374 CDIYGTIDFIFGDAVAVLQNCNIYVRRPMNGQKNTVTAQGRKDPNENTGIVIHNSNVMAT 433

Query: 429 PDLKPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLRTVYYGEFMNT 488
            D++PV+G + TYLGRPW+ YSRT+ M+S LDGLI+PAGWLPW+G+F L T+YYGE+MNT
Sbjct: 434 SDMRPVQGSFKTYLGRPWQKYSRTLFMKSNLDGLIDPAGWLPWSGNFALSTLYYGEYMNT 493

Query: 489 GGGANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
           G GA+T  RV WPGYHV+T   +A +FTV +FL G  W+P TGVPF +GL
Sbjct: 494 GSGASTARRVNWPGYHVITKATDAGKFTVGNFLAGDSWIPGTGVPFDSGL 529

BLAST of Lsi09G006730 vs. TAIR10
Match: AT1G23200.1 (AT1G23200.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 517.3 bits (1331), Expect = 1.1e-146
Identity = 283/560 (50.54%), Postives = 376/560 (67.14%), Query Frame = 1

Query: 2   KMLHSLLLFSLLVIIHGKSFNNFM--SCSQTPYPDLCFHYINPNDDVSTVNIDETSLITR 61
           K L++  + +++ ++     N     SC QTPYP +C H+++ N  + T++ D+T   T 
Sbjct: 11  KSLYTKCIITIIYVVSISHLNAHFITSCKQTPYPSVCDHHMS-NSPLKTLD-DQTDGFT- 70

Query: 62  FENLAIRATLNQAMVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINP 121
           F +L + +T++QA+ +H  + S     S      +  AL DCLELYED+I +LNHS  + 
Sbjct: 71  FHDLVVSSTMDQAVQLHRLVSSLKQHHSLHKHATS--ALFDCLELYEDTIDQLNHSRRSY 130

Query: 122 MSVNLI-DHSTLLSASLANYQTCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSNSLAI 181
              +   D  T LSA++AN  TC+ GF+DF  +   S + +FP Q   N +K +SNSLA+
Sbjct: 131 GQYSSPHDRQTSLSAAIANQDTCRNGFRDFKLT--SSYSKYFPVQFHRNLTKSISNSLAV 190

Query: 182 TKATAVAATT----------------SSTSGG--RRLLGGS---FPEWVYDDDRMLLQGA 241
           TKA A A                   SS  GG  RRLL  S   FP W    DR LL+ +
Sbjct: 191 TKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDS 250

Query: 242 VGN-RVDMVVAQDGSGDFRTISEAVAAVEEARKGSERFVIYVKGGIYKENVVIKKSMKNI 301
               + D+VVA+DGSG + +I +AV A  +  + ++R VIYVK G+Y+ENVVIKKS+KN+
Sbjct: 251 KTTAKADLVVAKDGSGHYTSIQQAVNAAAKLPRRNQRLVIYVKAGVYRENVVIKKSIKNV 310

Query: 302 MMIGDGMDRTIVTAMKNVQDGSTTFRSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRS 361
           M+IGDG+D TIVT  +NVQDG+TTFRSATFAV+G GFIA+ +TFENTAGPEKHQAVALRS
Sbjct: 311 MVIGDGIDSTIVTGNRNVQDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRS 370

Query: 362 NADRSIFYRCSFNGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVAVFQNCNIFVRKPMM 421
           ++D S+FY CSF GYQDTLY HS+RQF R C+IYGTVDFIFGD  A+ QNCNI+ RKPM 
Sbjct: 371 SSDFSVFYACSFKGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYARKPMS 430

Query: 422 NQKNTITAQARSDPNENSGFVIHNSVIGAAPDLKPVEGLYGTYLGRPWKAYSRTVVMRSF 481
            QKNTITAQ+R +P+E +GFVI +S +  A +         TYLGRPW+++SRTV M+  
Sbjct: 431 GQKNTITAQSRKEPDETTGFVIQSSTVATASE---------TYLGRPWRSHSRTVFMKCN 490

Query: 482 LDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGGGANTKGRVQWPGYHVLTSTAEAERFTVE 537
           L  L+ PAGWLPW+G F L T+YYGE+ NTG GA+  GRV+WPGYHV+ +  EAE+FTVE
Sbjct: 491 LGALVSPAGWLPWSGSFALSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVE 550

BLAST of Lsi09G006730 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 448.7 bits (1153), Expect = 4.9e-126
Identity = 242/510 (47.45%), Postives = 330/510 (64.71%), Query Frame = 1

Query: 27  CSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFENLAIRATLNQAMVVHDHLRSTMDLD 86
           CSQTP P  C +++  N +   +  +     + F  ++++  L++A++   H      L 
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSE-----SEFLKISMKLVLDRAILAKTH---AFTLG 93

Query: 87  SFDNDDKTKFALIDCLELYEDSIIELNHSTINPMSVNLIDHSTLLSASLANYQTCQEGFQ 146
               D + K A  DC++LY+ ++ ++N +    +  + +D  T LS +L N  TC+ GF 
Sbjct: 94  PKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFL 153

Query: 147 DFGFSFDDSSNFFFPFQILSNFSKLVSNSLAITKATAVAATTSSTSGGRRLLGGSFPEWV 206
           + G +     +   P  + +N S L+ N+LAI K      T     G        FP WV
Sbjct: 154 ELGVT-----DIVLPL-MSNNVSNLLCNTLAINKVP-FNYTPPEKDG--------FPSWV 213

Query: 207 YDDDRMLLQGAVGNRVDMVVAQDGSGDFRTISEAVAAVEEARKGSERFVIYVKGGIYKEN 266
              DR LLQ +   + + VVA+DGSG+F+TI EA+    +A  GS RFVIYVK G+Y EN
Sbjct: 214 KPGDRKLLQSSTP-KDNAVVAKDGSGNFKTIKEAI----DAASGSGRFVIYVKQGVYSEN 273

Query: 267 VVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGSTTFRSATFAVAGEGFIAKDMTFENTAGP 326
           + I+K  KN+M+ GDG+ +TI+T  K+V  G+TTF SAT A  G+GFIA+ +TF NTAG 
Sbjct: 274 LEIRK--KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGA 333

Query: 327 EKHQAVALRSNADRSIFYRCSFNGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGVAVFQN 386
              QAVALRS +D S+FY+CSF  YQDTLY HSNRQFYR+C +YGTVDFIFG+  AV QN
Sbjct: 334 SNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQN 393

Query: 387 CNIFVRKPMMNQKNTITAQARSDPNENSGFVIHNSVIGAAPDLKPVEGLYGTYLGRPWKA 446
           CNIF R+P  ++ NTITAQ RSDPN+N+G +IHNS + AA DL+PV G   TYLGRPW+ 
Sbjct: 394 CNIFARRPR-SKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQ 453

Query: 447 YSRTVVMRSFLDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGGGANTKGRVQWPGYHVLTS 506
           YSRTV M++ LD LI+P GWL W G+F L+T++Y EF NTG GA+T GRV WPG+ VL S
Sbjct: 454 YSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGS 511

Query: 507 TAEAERFTVESFLEGGDWLPATGVPFANGL 537
            +EA +FTV +FL GG W+P++ VPF +GL
Sbjct: 514 ASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Lsi09G006730 vs. TAIR10
Match: AT5G51490.1 (AT5G51490.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 426.0 bits (1094), Expect = 3.4e-119
Identity = 235/544 (43.20%), Postives = 327/544 (60.11%), Query Frame = 1

Query: 4   LHSLLLFSLLV--IIHGKSFNNFMSCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFE 63
           LH +LL  L V  +      N+   C +TPYPD C  Y   ++        + + ++ F 
Sbjct: 13  LHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHN-----GFQQPTQLSEFR 72

Query: 64  NLAIRATLNQAMVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHST--INP 123
            + + A +++A+     L ++        D K +  L DC++LY D+I++LN +   ++P
Sbjct: 73  VMLVEAAMDRAISARAELTNS---GKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSP 132

Query: 124 M-----SVNLIDHSTLLSASLANYQTCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSN 183
                 S    D  T LS +L N +TC+ G  D   +     +F  P    +  S L+SN
Sbjct: 133 KAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVT-----DFITPIVSNTKISHLISN 192

Query: 184 SLAITKATAVAATTSSTSGGRRLLGGSFPEWVYDDDRMLLQGAVGNRVDMVVAQDGSGDF 243
            LA+  A   A    +T+  ++     FP W+   D+ LL+     R ++VVA+DGSG F
Sbjct: 193 CLAVNGALLTAGNKGNTTANQK----GFPTWLSRKDKRLLRAV---RANLVVAKDGSGHF 252

Query: 244 RTISEAVAAVEEARKGSERFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNV 303
            T+  A+      +  S RFVIYVK GIY+EN+ ++ +  +IM++GDGM  TI+T  ++V
Sbjct: 253 NTVQAAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSV 312

Query: 304 QDGSTTFRSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDT 363
           Q G TT+ SAT  + G  FIAK +TF NTAGP K QAVALRS++D SIFY+CS  GYQDT
Sbjct: 313 QGGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDT 372

Query: 364 LYAHSNRQFYRECHIYGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENS 423
           L  HS RQFYREC+IYGTVDFIFG+  AVFQNC I  R+P+  Q N ITAQ R+DP +N+
Sbjct: 373 LMVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNT 432

Query: 424 GFVIHNSVIGAAPDLKPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPW--AGD 483
           G  IHNS I  APDLKPV G   TY+GRPW  +SRTVV++++LD ++ P GW PW     
Sbjct: 433 GISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSV 492

Query: 484 FGLRTVYYGEFMNTGGGANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPF 537
           FGL T++Y E+ NTG  ++T+ RV W G+HVL   ++A  FTV  F+ G  WLP TG+PF
Sbjct: 493 FGLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPF 536

BLAST of Lsi09G006730 vs. TAIR10
Match: AT3G60730.1 (AT3G60730.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 419.9 bits (1078), Expect = 2.4e-117
Identity = 225/456 (49.34%), Postives = 292/456 (64.04%), Query Frame = 1

Query: 95  KFALIDCLELYEDSIIELNHSTINPMSVNLIDHSTLLSASLANYQTCQEGF--QDFGFSF 154
           K  L +C +LY++S   L+   ++  +  + D  T LS  LAN+ TC +G   Q  G   
Sbjct: 66  KLGLSECEKLYDESEARLSKLVVDHENFTVEDVRTWLSGVLANHHTCLDGLIQQRQGHKP 125

Query: 155 DDSSNFFFPF-QILSNFSKLVSNSLAITKATAVAA----------TTSSTSGGRRLLGGS 214
              SN  F   + L+ + K   +        A             T  +   GR   G S
Sbjct: 126 LVHSNVTFVLHEALAFYKKSRGHMKKRLHGPARQGHGPTRPKHRPTRPNHGPGRSHHGPS 185

Query: 215 FPEWVYDDDRMLLQGAVGNRVDMVVAQDGSGDFRTISEAVAAVEEARKGS-ERFVIYVKG 274
            P    +   ++      +R D VVA+DGS   RTI++A+AAV    K    R +IY+K 
Sbjct: 186 RPN--QNGGMLVSWNPTSSRADFVVARDGSATHRTINQALAAVSRMGKSRLNRVIIYIKA 245

Query: 275 GIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGSTTFRSATFAVAGEGFIAKDMTF 334
           G+Y E + I + MKNIM++GDGMDRTIVT  +NV DGSTT+ SATF V+G+GF A+D+TF
Sbjct: 246 GVYNEKIEIDRHMKNIMLVGDGMDRTIVTNNRNVPDGSTTYGSATFGVSGDGFWARDITF 305

Query: 335 ENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYAHSNRQFYRECHIYGTVDFIFGDG 394
           ENTAGP KHQAVALR ++D S+FYRCSF GYQDTL+ HS RQFYR+CHIYGT+DFIFGD 
Sbjct: 306 ENTAGPHKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDA 365

Query: 395 VAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFVIHNSVIGAAPDLKPVEGLYGTYL 454
            AVFQNC+IFVR+PM +Q N ITAQ R DP+ NSG  I +S I AAP+ + V+G + +YL
Sbjct: 366 AAVFQNCDIFVRRPMDHQGNMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYL 425

Query: 455 GRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGGGANTKGRVQWPG 514
           GRPWK YSRTV +++ +D LI+P GW  W+G + L T+YYGEFMNTG GA T  RV WPG
Sbjct: 426 GRPWKKYSRTVFLKTDIDELIDPRGWREWSGSYALSTLYYGEFMNTGAGAGTGRRVNWPG 485

Query: 515 YHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
           +HVL    EA  FTV  F++G  W+P TGVPF+ G+
Sbjct: 486 FHVLRGEEEASPFTVSRFIQGDSWIPITGVPFSAGV 519

BLAST of Lsi09G006730 vs. TAIR10
Match: AT3G47400.1 (AT3G47400.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 419.1 bits (1076), Expect = 4.2e-117
Identity = 224/521 (42.99%), Postives = 320/521 (61.42%), Query Frame = 1

Query: 27  CSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFENLAIRATLNQAMVVHDHLRSTMDLD 86
           C++TP+ + C +Y   +   + +N+      + F  + ++  L+QA++ H     T+   
Sbjct: 88  CNKTPHAETCNYYFRKSSQ-NNINLRPPRFRSEFLRMLVKVALDQAVITHSQ---TVKFG 147

Query: 87  SFDNDDKTKFALIDCLELYEDSIIELNHST--INPMSVNLI-----DHSTLLSASLANYQ 146
               +++ K A  DC+ L+++++ +LN +   +NP + + +     D  T LS +  N +
Sbjct: 148 PSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIE 207

Query: 147 TCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSNSLAITKATAVAATTSSTSGGRRLLG 206
           TC+ G +D   S     +F  P     N S L+ N LA+          ++T+  +    
Sbjct: 208 TCRSGSEDLNVS-----DFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEY-- 267

Query: 207 GSFPEWVYDDDRMLLQGA--VGNRVDMVVAQDGSGDFRTISEAVAAVEEARKGSERFVIY 266
             FP WV   +R LL  A    +   +VVAQD SG FR+I  A+      R+   RFVIY
Sbjct: 268 --FPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAAR-RRFKSRFVIY 327

Query: 267 VKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGSTTFRSATFAVAGEGFIAKD 326
           VK G+Y+EN+ +     NIM++GDG  +TI+T+ ++VQ G TT+ SAT    G+ F+AKD
Sbjct: 328 VKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKD 387

Query: 327 MTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYAHSNRQFYRECHIYGTVDFIF 386
           MTF NTAGP + QAVA+RS++D S+FYR   +G+QDTLY HS RQF+REC+I GT+DFIF
Sbjct: 388 MTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIF 447

Query: 387 GDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFVIHNSVIGAAPDLKPVEGLYG 446
           G+   VFQNC I VR+P+  Q N ITAQ R DP +N+G  IH+S I AA DLKPV   Y 
Sbjct: 448 GNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYK 507

Query: 447 TYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPW--AGDFGLRTVYYGEFMNTGGGANTKGR 506
           TYLGRPW+AYSR  +M++++D  I P GW PW    +F L TV+YGE+ N G G++T+ R
Sbjct: 508 TYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWR 567

Query: 507 VQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
           V+W G+H +TSTA A RFTV S + GG WLPATGVPF +GL
Sbjct: 568 VRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Lsi09G006730 vs. NCBI nr
Match: gi|449464802|ref|XP_004150118.1| (PREDICTED: pectinesterase [Cucumis sativus])

HSP 1 Score: 914.1 bits (2361), Expect = 1.2e-262
Identity = 454/539 (84.23%), Postives = 487/539 (90.35%), Query Frame = 1

Query: 1   MKMLHSLLLFSLLVIIHGKSFNNFMSCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRF 60
           MK+LH  LLFSLLVIIHG+SFNNFMSCSQTPYPDLCFHYINPND++ T +IDET LITRF
Sbjct: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60

Query: 61  ENLAIRATLNQAMVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINPM 120
            N AI+ATL QAM V DHLRSTMDL SFD++D+   ALIDCLE YEDSI ELN ST++  
Sbjct: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120

Query: 121 SVNLIDHSTLLSASLANYQTCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSNSLAITK 180
           S N IDHST+LSASLAN+QTC +GF+DFGF  DDS+NFF P QI+SNFSKLVSNSLAITK
Sbjct: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 ATAVA-ATTSSTSGGRRLLGGSFPEWVYDDDRMLLQGAVGNRVDMVVAQDGSGDFRTISE 240
           A A A +T+SSTS G+ LL G  PEWVYDDD+ LLQ  VG   DMVVAQDGSGDFRTISE
Sbjct: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240

Query: 241 AVAAVEEARKGSE--RFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDG 300
           AVAA EEARKGS   RFVIYVKGGIYKENVVI+K MKNIMM+GDGMDRTIVTAMKNVQDG
Sbjct: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300

Query: 301 STTFRSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYA 360
           STTF+SATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSF GYQDTLYA
Sbjct: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360

Query: 361 HSNRQFYRECHIYGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFV 420
           HSNRQFYRECHIYGTVDFIFGDGV VFQNCNIFVRKPM+NQKNTITAQARSDPNENSGFV
Sbjct: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420

Query: 421 IHNSVIGAAPDLKPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLRT 480
           IHNSVIGAAPDLKPV+GLYGTYLGRPWKAYSRTV+M+S++DGLIEPAGWLPWAGDFGLRT
Sbjct: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480

Query: 481 VYYGEFMNTGGGANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
           VYYGEFMNTG GANTKGRV+WPGYHV+TS  EAERFTVESFLEGG W+PATGVPF NGL
Sbjct: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 539

BLAST of Lsi09G006730 vs. NCBI nr
Match: gi|659072831|ref|XP_008467117.1| (PREDICTED: pectinesterase [Cucumis melo])

HSP 1 Score: 879.0 bits (2270), Expect = 4.2e-252
Identity = 435/538 (80.86%), Postives = 473/538 (87.92%), Query Frame = 1

Query: 1   MKMLHSLLLFSLLVIIHGKSFNNFMSCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRF 60
           MK ++  LLFSLLVIIHG+SFNNFMSC QTPYPDLCFHYINPND++ T +IDET L TRF
Sbjct: 1   MKFVYCFLLFSLLVIIHGRSFNNFMSCFQTPYPDLCFHYINPNDNIPTADIDETYLNTRF 60

Query: 61  ENLAIRATLNQAMVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINPM 120
            N A++ATLNQA  VHDHLRS MDL SFD+D++ K ALIDCLE YEDSI +LN S  +  
Sbjct: 61  RNSAVQATLNQARKVHDHLRSIMDLGSFDDDNRNKVALIDCLEFYEDSIAKLNRSVFSST 120

Query: 121 SVNLIDHSTLLSASLANYQTCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSNSLAITK 180
           SVN IDH T+LSASLAN+QTC +GF+DF    D+S+NFF P QI+SNFSKLVSNSLAITK
Sbjct: 121 SVNSIDHLTMLSASLANHQTCLDGFRDF----DNSNNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 ATAVAATTSSTSGGRRLLGGSFPEWVYDDDRMLLQGAVGNRVDMVVAQDGSGDFRTISEA 240
           A    A++SSTS G+ L  G  PEWVYDDD+ LL+  VG   DMVVAQDGSGDFRTISEA
Sbjct: 181 AAVAPASSSSTSCGQPLPDGLCPEWVYDDDKTLLRDMVGKGADMVVAQDGSGDFRTISEA 240

Query: 241 VAAVEEARK--GSERFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGS 300
           VAA EEARK  GSERFVIYVKGGIYKENVVIKKSMKN+MMIGDGMDRTI+TAMKNVQDGS
Sbjct: 241 VAAAEEARKGSGSERFVIYVKGGIYKENVVIKKSMKNVMMIGDGMDRTIITAMKNVQDGS 300

Query: 301 TTFRSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYAH 360
           TTF+SATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSF GYQDTLYAH
Sbjct: 301 TTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAH 360

Query: 361 SNRQFYRECHIYGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFVI 420
           SNRQFYRECHIYGTVDFIFGDG  VFQNCNIFVRKPMMNQKNTITAQ RSDPNEN+GFVI
Sbjct: 361 SNRQFYRECHIYGTVDFIFGDGAVVFQNCNIFVRKPMMNQKNTITAQGRSDPNENTGFVI 420

Query: 421 HNSVIGAAPDLKPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLRTV 480
           HNSVI AAPDLKPV+GLYGTYLGRPWKAYSRTV+M+S+LDGLIEPAGWLPWAGDFGLRT+
Sbjct: 421 HNSVIKAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYLDGLIEPAGWLPWAGDFGLRTI 480

Query: 481 YYGEFMNTGGGANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
           YYGEFMN GGGANTKGRV+WPGYHV+TS  EAE FTVESFL G  W+P TGVPF NG+
Sbjct: 481 YYGEFMNIGGGANTKGRVRWPGYHVMTSATEAEGFTVESFLGGRGWIPTTGVPFVNGV 534

BLAST of Lsi09G006730 vs. NCBI nr
Match: gi|731369412|ref|XP_010654104.1| (PREDICTED: pectinesterase [Vitis vinifera])

HSP 1 Score: 603.6 bits (1555), Expect = 3.4e-169
Identity = 318/538 (59.11%), Postives = 392/538 (72.86%), Query Frame = 1

Query: 3   MLHSLLLFSLLVIIHGKSFNNFMSCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFEN 62
           +LH L  + +++ +         SCSQTPYP++C ++I   +   T  IDE      F +
Sbjct: 5   LLHFLSAYFVVLYVRVVDGAPISSCSQTPYPEVCNYFIG--NYKPTAGIDEIQF--PFRD 64

Query: 63  LAIRATLNQAMVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINPMSV 122
             +  T+NQA  +H  L S MDL S  +D++TK A  DCLELYE++I  +N S  +    
Sbjct: 65  RVLGVTMNQAKRLH-LLVSAMDLSS--SDERTKLAWADCLELYENTIDLVNRSINSISPA 124

Query: 123 NLIDHSTLLSASLANYQTCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSNSLAITKAT 182
            + D  T LSA++AN QTC +GF DF  S D   +F       SNFSKL+SNSLAI KA 
Sbjct: 125 VMFDSQTWLSAAIANQQTCLDGFIDFNPSSDQFQSFPSMSISTSNFSKLLSNSLAINKA- 184

Query: 183 AVAATT---SSTSGGRRLLGGSFPEWVYDDDRMLLQGA-VGNRVDMVVAQDGSGDFRTIS 242
           AV+AT+   ++ +GGRRLL   FP WV   DR LLQ +   +R D+VVA DGSG+++TIS
Sbjct: 185 AVSATSILSNNQAGGRRLLSNGFPTWVSAADRKLLQSSGAASRADIVVAHDGSGNYKTIS 244

Query: 243 EAVAAVEEARKGSERFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGS 302
           EAVAA  + R G++RFVIYVK G+Y+ENV IK+ MKNIM+IGDG D TIVT  KNVQDGS
Sbjct: 245 EAVAASVKLRSGTKRFVIYVKAGVYRENVEIKRKMKNIMIIGDGKDATIVTGNKNVQDGS 304

Query: 303 TTFRSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYAH 362
           TTFRSATFAV+G GFIA+DMTFENTAGPEKHQAVALRS++D S+FY CSF GYQDTLY H
Sbjct: 305 TTFRSATFAVSGHGFIARDMTFENTAGPEKHQAVALRSSSDGSVFYGCSFKGYQDTLYVH 364

Query: 363 SNRQFYRECHIYGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFVI 422
           + RQFYR C +YGTVDFIFGD VAV QNCNI+VR+PM NQ N ITAQ RSD NEN+G  I
Sbjct: 365 TQRQFYRSCDVYGTVDFIFGDAVAVLQNCNIYVRRPMSNQANVITAQGRSDQNENTGISI 424

Query: 423 HNSVIGAAPDLKPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLRTV 482
           HNS + AAPDL+PV+  + TYLGRPW+ YSRTV M++ LDGLI P GW PW GDFGL T+
Sbjct: 425 HNSRVMAAPDLRPVQSRFKTYLGRPWRKYSRTVFMKTSLDGLIHPEGWSPWKGDFGLSTL 484

Query: 483 YYGEFMNTGGGANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
           YYGE+MNTG GA+T+GRV+W GYHV+TS AEA++FTV  FL G  W+P TGVP+A+GL
Sbjct: 485 YYGEYMNTGSGASTRGRVKWRGYHVITSAAEADKFTVGRFLVGDSWIPTTGVPYASGL 534

BLAST of Lsi09G006730 vs. NCBI nr
Match: gi|703119879|ref|XP_010101977.1| (putative pectinesterase/pectinesterase inhibitor 6 [Morus notabilis])

HSP 1 Score: 601.3 bits (1549), Expect = 1.7e-168
Identity = 313/527 (59.39%), Postives = 392/527 (74.38%), Query Frame = 1

Query: 13  LVIIHGKSFNNFMSCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFENLAIRATLNQA 72
           + ++HG   +   +C+QTPYP +C H++N N  +ST++ D+T++   F +LA++AT+ QA
Sbjct: 1   MYVVHGTK-STISTCNQTPYPQVCHHFLNTNH-LSTLD-DKTTV--SFHDLALKATMKQA 60

Query: 73  MVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINPMSVNLIDHSTLLS 132
           +  H  L STMD DSF   ++ K A  DCLELY+D++  LN S +N  + N +D  T LS
Sbjct: 61  VEAH-RLVSTMDSDSFG--ERGKSAWQDCLELYKDTVDLLNRS-VNSNNKNPLDTHTWLS 120

Query: 133 ASLANYQTCQEGFQDFGFSFDDSSNFFFPFQILSNFSKLVSNSLAITKATAVAATTSSTS 192
           ASLAN  TC+ GF DF       S   FP  +LSNFSKL+SNSLAI KAT  +  TS + 
Sbjct: 121 ASLANQHTCRNGFTDFQIPSHYDS---FP-SMLSNFSKLLSNSLAINKATIPSTATSFSK 180

Query: 193 G--GRRLLGGSFPEWVYDDDRMLLQ-GAVGNRVDMVVAQDGSGDFRTISEAVAAVEEARK 252
               RRLL   FPEWV   DR LLQ G    + D+VVAQDGSG F+TISEAV A +    
Sbjct: 181 QVRSRRLLSDGFPEWVSAADRKLLQSGGAAAKADIVVAQDGSGGFKTISEAVEAAKNLGG 240

Query: 253 GSERFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNVQDGSTTFRSATFAVA 312
           GS+R V+YVK G+YKENV IK  +KN+M +GDG+D TIVT  +N  DGSTTF+SATF V+
Sbjct: 241 GSKRIVVYVKAGVYKENVEIKNDIKNLMFVGDGIDATIVTGNRNNVDGSTTFQSATFGVS 300

Query: 313 GEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDTLYAHSNRQFYRECHI 372
           G+GFIA+DMTFENTAGP KHQAVALRS +D S+F+RCSF GYQDTLY +S RQFYR+C++
Sbjct: 301 GDGFIAQDMTFENTAGPAKHQAVALRSGSDHSVFHRCSFKGYQDTLYVYSQRQFYRDCNV 360

Query: 373 YGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENSGFVIHNSVIGAAPDL 432
           YGTVDFI GD VA+ QNCNI VRKPM +Q+NT+T+QAR+DPNEN+G +IHNS I A+ DL
Sbjct: 361 YGTVDFICGDAVAILQNCNILVRKPMSDQRNTVTSQARTDPNENTGIIIHNSRITASSDL 420

Query: 433 KPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLRTVYYGEFMNTGGG 492
           +PV+G + TYLGRPW+ YSRTV+M+S LDGLI+P GWLPW+G+F L T+YY EFMN+G G
Sbjct: 421 RPVQGSFETYLGRPWQKYSRTVIMKSSLDGLIDPKGWLPWSGNFALSTLYYAEFMNSGAG 480

Query: 493 ANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFANGL 537
           A T GRV WPGYHVLTS AEA +FTV +FL G  W+PATGVPFA GL
Sbjct: 481 AATGGRVSWPGYHVLTSAAEAGKFTVGNFLGGDSWIPATGVPFAAGL 514

BLAST of Lsi09G006730 vs. NCBI nr
Match: gi|224108303|ref|XP_002314796.1| (pectin methylesterase 6 family protein [Populus trichocarpa])

HSP 1 Score: 589.3 bits (1518), Expect = 6.6e-165
Identity = 305/542 (56.27%), Postives = 391/542 (72.14%), Query Frame = 1

Query: 4   LHSLLLFSLLVIIHGKSFNNFMSCSQTPYPDLCFHYINPNDDVSTVNIDETSLITRFENL 63
           L++  LF  L ++HG S     SC QTP+P+ C ++I+        NI +T  +    + 
Sbjct: 11  LNAYFLFYSLALVHGDSVT---SCDQTPFPEACNYFID-------TNISKTPPLFALRDQ 70

Query: 64  AIRATLNQAMVVHDHLRSTMDLDSFDNDDKTKFALIDCLELYEDSIIELNHSTINPMSVN 123
           ++  T+N+A+  H  + S+M+L SF+   + K A  DCL+LYED++  +N S     S N
Sbjct: 71  SLSITMNKAIEAHQMV-SSMELSSFNQ--QAKLAWDDCLKLYEDTVDHVNRSM---SSNN 130

Query: 124 LIDHSTLLSASLANYQTCQEGFQDFGF-SFDDSSNFFFPFQILSNFSKLVSNSLAITKA- 183
           L D  T LSA++AN +TC+ GF DF   S+ +S        +L NF+KL+SN+L++ KA 
Sbjct: 131 LADSQTWLSAAIANQRTCENGFIDFNIVSYLESLP-----NMLRNFTKLLSNTLSLNKAI 190

Query: 184 ---TAVAATTSSTSGGRRLLGGSFPEWVYDDDRMLLQ--GAVGNRVDMVVAQDGSGDFRT 243
              T +   T    G RRLL   FP WV   DR LLQ  G    + D+VVAQDGSGD++T
Sbjct: 191 ISSTPILLDTKQDGGRRRLLVDGFPSWVPASDRKLLQSNGRAAPKADIVVAQDGSGDYKT 250

Query: 244 ISEAVAAVEEARKG--SERFVIYVKGGIYKENVVIKKSMKNIMMIGDGMDRTIVTAMKNV 303
           ISEAVAA  + R G  S+RFVIYVKGG+YKENV IKKSMKN+M +GDG+D T++T+ KN 
Sbjct: 251 ISEAVAASAKLRSGTKSKRFVIYVKGGVYKENVEIKKSMKNLMFVGDGIDATVITSNKNT 310

Query: 304 QDGSTTFRSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFNGYQDT 363
           QDG+TTFRSAT  V+G+GFIA+D+TFENTAGP+KHQAVALRS +D S+FY CSF GYQDT
Sbjct: 311 QDGTTTFRSATVGVSGKGFIARDITFENTAGPQKHQAVALRSGSDFSVFYSCSFKGYQDT 370

Query: 364 LYAHSNRQFYRECHIYGTVDFIFGDGVAVFQNCNIFVRKPMMNQKNTITAQARSDPNENS 423
           LY HS RQFYR+C IYGTVDFIFGD VAV QNCNI+VR+PM  Q NT+TAQ R+DP+EN+
Sbjct: 371 LYVHSQRQFYRDCDIYGTVDFIFGDAVAVLQNCNIYVRRPMSKQTNTVTAQGRTDPDENT 430

Query: 424 GFVIHNSVIGAAPDLKPVEGLYGTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFG 483
           G VIHNS + AAPDL+PV+G + +YLGRPWK YSRTV ++S +DGLI+PAGWLPW GDF 
Sbjct: 431 GIVIHNSRVMAAPDLRPVQGSFKSYLGRPWKKYSRTVFLKSNIDGLIDPAGWLPWKGDFA 490

Query: 484 LRTVYYGEFMNTGGGANTKGRVQWPGYHVLTSTAEAERFTVESFLEGGDWLPATGVPFAN 537
           L T+YYGE+M+TG GA+TKGRV+WPGYH +TS  EA +FTVE+FL G  W+ A GVPF +
Sbjct: 491 LSTLYYGEYMSTGSGASTKGRVKWPGYHTITSPLEAGKFTVENFLAGNSWISAAGVPFES 531