Cp4.1LG13g03580 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG13g03580
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectinesterase
LocationCp4.1LG13 : 3683843 .. 3686090 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGATCACCCATGCCCAAAGCTTCCACAATTTCATGTCTTGCTCCCAAACCCCATATCCAGACCTCTGCTTCCATTTCATCAACCCGAACCACAACGTACCCACCACCGACGTCCATCAATCTTATAACATCACTCGCTTTCGAAACTCGGCCATTCGAGCTACTTTGAACCAAGCCATGGTGGCCCATCACCACCTTCTCTCTGCCATGGATTTAGCTTCCTTTGACGATGACGACCGAGTGAAATCAGCTTTAATCGACTGTCTGGAACTCTATGAGGATTCAATCACCGAGCTCAATCGCTCGGTGATGAACTCTATGAGGGCCAATTCAATTGACCACTTGACATTGCTAAGTGCTTCATTAGCGAATCATCAAACATGTCGAGATGGGTTTCGAGATTTTGGATTTTCTTCTCATTATTTGAATAATATCATTTTTCCACATCAAATTTTGAGTAATTTCTCGAAATTGATCACCAATTCTCTTGCCATCACCAAGGCCGCTGCCGTGACAGCTACTACTTCTTCCAATCCTCGTAGCCAGCGGCTACTAGCCGGGTCGTTTCCCAAGTGGGTTTCTAGTGATGATAGGATGCTCCTCCGGGACACGACCGAGAAGCGCGCGGATATGGTGGTGGCCCAAGATGGGAGCGGCAATTTCAGAACGATATCGGAGGCGGTGGCCGCGGCGGCCGAGGTTAGGAAGGGAAGTGAAAGAAGGTTTGTTATATATGTAAAAGGTGGGATTTACAGAGAGAATGTTGTGATTAAGAAAACGTTGAAGAATGTTATGATGATTGGAGATGGGATGGGTGAGACGATCGTTACAGCCATGAAAAACGTCAAAGATGGATCAACCACTTTCCGATCAGCCACTTTTGGTTCGTAGATCCTCATGTTTTGATCCTATTTTTATGTTAATTTTGCTTCCATGCATGCATGCAAATGGATCGACTAGTTATAGTTTCCTATCAATTTCTTTACTATCAATTTGTTTTCTAAGAGTTTTAGAACCTTTAATTTGATCAAATATAATCAATTAATATGGCTTAGCCAAAAATAATAATAATAATCAAAATAAAAAAATTCAACTCATGGACCAAATTGATTGAATTACCAAGTCATAACTCATCGGAAAAAATGTTGGCGGCAATTTCAGCGGTGGTCGGCGAAGGGTTCATCGCCAAAGACATGACGTTCGAGAACAGGGCAGGAGCAGAGAAGCACCAAGCTGTTTCTCTCCGAACTAACGCCGATCGCTCAATTTTCTACCGATGCAGCTTCAAAGGCTACCAAGACACTCTATACGCCCACTCCAACCGCCATTTCTTCCGTGAATGCCACATCTACGGAACCATCGACTTCATTTTCGGCGATGGCGCCTCTGTATTTCAAAATTGCAACATCTTCGTCAGAAAGCCGATGGAAAACCAGAAGAACACGGTGACGGCGCAGGGGAGGTCCGACCCGAATGAAAACACGGGGTTTGTGATACATAATTCCGTTGTCAGAGCGGCACCGGACCTGAAGGCGGTGGAGGGTGTGTACAAAACCTATTTGGGTAGGCCGTGGAAGGCGTATTCGAGGACGGTGGTAATGAGGAGCTTTTTGGATGGGCTAATTGAACCGGCGGGGTGGCTTCCTTGGGCAGGAGATTTCGGTCTGAAGACGGTTTATTATGGGGAGTTTAAGAACAGCGGTGGCGGGGCAAGTACAATGAGGAGGGTGCAGTGGCCGGGTTACCATGTCATGAAGACTGCGGCGGAGGCCGAGAGATTTACAGTGGAAAGTTTCCTGGAAGGAGGAGGTTGGATTCCGGCCACCGGAGTGCCATTTGCAAATGGGGTATGATTATGAAATAGAAGTTGACAGAAAAAATATATGGATGTACATCGGTCAATGGTTGTTGCTGCTGTACATTTTGAAAGCGGTGTACCATAAAAGTTAATGGTGGTAAGAAATAAGGTCTATTTGGAAGAGAAAAATGGGATGGGGGAAAAAAGTTAGGCAGTGCAAAAAGATGTAAGAAAAATGTTGTGCAATTTGTGTTTGGATTTGTTTGGGGACAAAAAAGTTGGGTATATAGCTTATTATAGTTGGAAAACTTTTATATTTCGTTTTTCCCTTCTTTTCATTCTATTATGGAAGACTAAATTAATGTAATAAATAGTTATCAATCAGGATTAGATCCTCATTTTGGTTTCCTATTTTTCCTTCCTAGTTCTTAAAGCACAGGCAGTTGATAGA

mRNA sequence

GGATCACCCATGCCCAAAGCTTCCACAATTTCATGTCTTGCTCCCAAACCCCATATCCAGACCTCTGCTTCCATTTCATCAACCCGAACCACAACGTACCCACCACCGACGTCCATCAATCTTATAACATCACTCGCTTTCGAAACTCGGCCATTCGAGCTACTTTGAACCAAGCCATGGTGGCCCATCACCACCTTCTCTCTGCCATGGATTTAGCTTCCTTTGACGATGACGACCGAGTGAAATCAGCTTTAATCGACTGTCTGGAACTCTATGAGGATTCAATCACCGAGCTCAATCGCTCGGTGATGAACTCTATGAGGGCCAATTCAATTGACCACTTGACATTGCTAAGTGCTTCATTAGCGAATCATCAAACATGTCGAGATGGGTTTCGAGATTTTGGATTTTCTTCTCATTATTTGAATAATATCATTTTTCCACATCAAATTTTGAGTAATTTCTCGAAATTGATCACCAATTCTCTTGCCATCACCAAGGCCGCTGCCGTGACAGCTACTACTTCTTCCAATCCTCGTAGCCAGCGGCTACTAGCCGGTCATAACTCATCGGAAAAAATGTTGGCGGCAATTTCAGCGGTGGTCGGCGAAGGGTTCATCGCCAAAGACATGACGTTCGAGAACAGGGCAGGAGCAGAGAAGCACCAAGCTGTTTCTCTCCGAACTAACGCCGATCGCTCAATTTTCTACCGATGCAGCTTCAAAGGCTACCAAGACACTCTATACGCCCACTCCAACCGCCATTTCTTCCGTGAATGCCACATCTACGGAACCATCGACTTCATTTTCGGCGATGGCGCCTCTGTATTTCAAAATTGCAACATCTTCGTCAGAAAGCCGATGGAAAACCAGAAGAACACGGTGACGGCGCAGGGGAGGTCCGACCCGAATGAAAACACGGGGAGGAGGTTGGATTCCGGCCACCGGAGTGCCATTTGCAAATGGGTTCTTAAAGCACAGGCAGTTGATAGA

Coding sequence (CDS)

ATGTCTTGCTCCCAAACCCCATATCCAGACCTCTGCTTCCATTTCATCAACCCGAACCACAACGTACCCACCACCGACGTCCATCAATCTTATAACATCACTCGCTTTCGAAACTCGGCCATTCGAGCTACTTTGAACCAAGCCATGGTGGCCCATCACCACCTTCTCTCTGCCATGGATTTAGCTTCCTTTGACGATGACGACCGAGTGAAATCAGCTTTAATCGACTGTCTGGAACTCTATGAGGATTCAATCACCGAGCTCAATCGCTCGGTGATGAACTCTATGAGGGCCAATTCAATTGACCACTTGACATTGCTAAGTGCTTCATTAGCGAATCATCAAACATGTCGAGATGGGTTTCGAGATTTTGGATTTTCTTCTCATTATTTGAATAATATCATTTTTCCACATCAAATTTTGAGTAATTTCTCGAAATTGATCACCAATTCTCTTGCCATCACCAAGGCCGCTGCCGTGACAGCTACTACTTCTTCCAATCCTCGTAGCCAGCGGCTACTAGCCGGTCATAACTCATCGGAAAAAATGTTGGCGGCAATTTCAGCGGTGGTCGGCGAAGGGTTCATCGCCAAAGACATGACGTTCGAGAACAGGGCAGGAGCAGAGAAGCACCAAGCTGTTTCTCTCCGAACTAACGCCGATCGCTCAATTTTCTACCGATGCAGCTTCAAAGGCTACCAAGACACTCTATACGCCCACTCCAACCGCCATTTCTTCCGTGAATGCCACATCTACGGAACCATCGACTTCATTTTCGGCGATGGCGCCTCTGTATTTCAAAATTGCAACATCTTCGTCAGAAAGCCGATGGAAAACCAGAAGAACACGGTGACGGCGCAGGGGAGGTCCGACCCGAATGAAAACACGGGGAGGAGGTTGGATTCCGGCCACCGGAGTGCCATTTGCAAATGGGTTCTTAAAGCACAGGCAGTTGATAGA

Protein sequence

MSCSQTPYPDLCFHFINPNHNVPTTDVHQSYNITRFRNSAIRATLNQAMVAHHHLLSAMDLASFDDDDRVKSALIDCLELYEDSITELNRSVMNSMRANSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLITNSLAITKAAAVTATTSSNPRSQRLLAGHNSSEKMLAAISAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGRRLDSGHRSAICKWVLKAQAVDR
Homology
[Close]
BLAST of Cp4.1LG13g03580 vs. Swiss-Prot
Match: PME47_ARATH (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana GN=PME47 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.2e-41
Identity = 78/109 (71.56%), Postives = 91/109 (83.49%), Query Frame = 1 

Query: 189 AVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRE 248
           AVVG+ F+A D+TF N AG EKHQAV++R NAD S FYRCSF+GYQDTLY HS R F+RE
Sbjct: 392 AVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQDTLYVHSLRQFYRE 451

Query: 249 CHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 298
           C IYGTIDFIFG+ A++FQNCNI+ RKPM NQKN VTA GR+DPN+ TG
Sbjct: 452 CDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQKTG 500

[Close]
BLAST of Cp4.1LG13g03580 vs. Swiss-Prot
Match: PME25_ARATH (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=PME25 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 6.1e-41
Identity = 77/109 (70.64%), Postives = 90/109 (82.57%), Query Frame = 1 

Query: 189 AVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRE 248
           AVVGE F+A D+TF N AG EKHQAV+LR NA+ S FYRCSF+GYQDTLY HS R F+RE
Sbjct: 387 AVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLYVHSLRQFYRE 446

Query: 249 CHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 298
           C IYGT+DFIFG+ A++FQNCNI+ RKPM  QKN +TA GR DPN+NTG
Sbjct: 447 CDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTG 495

[Close]
BLAST of Cp4.1LG13g03580 vs. Swiss-Prot
Match: PME36_ARATH (Probable pectinesterase/pectinesterase inhibitor 36 OS=Arabidopsis thaliana GN=PME36 PE=2 SV=2)

HSP 1 Score: 169.1 bits (427), Expect = 7.9e-41
Identity = 76/113 (67.26%), Postives = 95/113 (84.07%), Query Frame = 1 

Query: 185 AAISAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRH 244
           +A   V G+GF A+D+TFEN AG  KHQAV+LR ++D S+FYRCSFKGYQDTL+ HS R 
Sbjct: 286 SATFGVSGDGFWARDITFENTAGPHKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQ 345

Query: 245 FFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 298
           F+R+CHIYGTIDFIFGD A+VFQNC+IFVR+PM++Q N +TAQGR DP+ N+G
Sbjct: 346 FYRDCHIYGTIDFIFGDAAAVFQNCDIFVRRPMDHQGNMITAQGRDDPHTNSG 398

[Close]
BLAST of Cp4.1LG13g03580 vs. Swiss-Prot
Match: PME6_ARATH (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana GN=PME6 PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.5e-39
Identity = 74/113 (65.49%), Postives = 91/113 (80.53%), Query Frame = 1 

Query: 185 AAISAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRH 244
           +A  AV G GFIA+ +TFEN AG EKHQAV+LR+++D S+FY CSFKGYQDTLY HS+R 
Sbjct: 330 SATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQ 389

Query: 245 FFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 298
           F R C+IYGT+DFIFGD  ++ QNCNI+ RKPM  QKNT+TAQ R +P+E TG
Sbjct: 390 FLRNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTG 442

[Close]
BLAST of Cp4.1LG13g03580 vs. Swiss-Prot
Match: PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.3e-39
Identity = 74/109 (67.89%), Postives = 87/109 (79.82%), Query Frame = 1 

Query: 189 AVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRE 248
           A  G GFIA+D+TFEN AG  KHQAV+LR  AD ++ YRC+  GYQDTLY HSNR FFRE
Sbjct: 367 AATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRE 426

Query: 249 CHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 298
           C IYGT+DFIFG+ A V QNC+I+ RKPM+ QKNT+TAQ R DPN+NTG
Sbjct: 427 CDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTG 475

[Close]
BLAST of Cp4.1LG13g03580 vs. TrEMBL
Match: A0A0A0KUU2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G568300 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.9e-55
Identity = 120/177 (67.80%), Postives = 135/177 (76.27%), Query Frame = 1 

Query: 1   MSCSQTPYPDLCFHFINPNHNVPTTDVHQSYNITRFRNSAIRATLNQAMVAHHHLLSAMD 60
           MSCSQTPYPDLCFH+INPN N+ T  + ++Y ITRFRNSAI+ATL QAM    HL S MD
Sbjct: 25  MSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQAMKVRDHLRSTMD 84

Query: 61  LASFDDDDRVKSALIDCLELYEDSITELNRSVMNSMRANSIDHLTLLSASLANHQTCRDG 120
           L SFDD+DR   ALIDCLE YEDSI ELNRS ++S  ANSIDH T+LSASLANHQTC DG
Sbjct: 85  LGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSIDHSTMLSASLANHQTCLDG 144

Query: 121 FRDFGFSSHYLNNIIFPHQILSNFSKLITNSLAITKA-AAVTATTSSNPRSQRLLAG 177
           FRDFGF     NN   P QI+SNFSKL++NSLAITKA AA  +T+SS    Q LL G
Sbjct: 145 FRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAAAPSTSSSTSCGQPLLDG 201

[Close]
BLAST of Cp4.1LG13g03580 vs. TrEMBL
Match: I1JKS0_SOYBN (Pectinesterase OS=Glycine max PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 4.4e-46
Identity = 127/338 (37.57%), Postives = 184/338 (54.44%), Query Frame = 1 

Query: 3   CSQTPYPDLCFHFINPNH--NVPTTDVHQSYNITRFRNSAIRATLNQAMV-AHHHLLSAM 62
           CSQTPYP  C +++  NH  N P           + ++  ++ +L  A+  A     +  
Sbjct: 34  CSQTPYPQPCEYYLT-NHAFNQPI----------KSKSDFLKVSLQLALERAQRSEFNTH 93

Query: 63  DLASFDDDDRVKSALIDCLELYEDSITELNRSVMNSMRANSIDHLTLLSASLANHQTCRD 122
            L     +   KSA  DCLELYE +I +LN+++    +    D  T LS +L N +TC++
Sbjct: 94  ALGPKCRNVHEKSAWADCLELYEYTIQKLNKTIAPYTKCTQTDTQTWLSTALTNLETCKN 153

Query: 123 GFRDFGFSSHYLNNIIFPHQILSNFSKLITNSLAI--------TKAAAVTATTSSNPR-- 182
           GF + G   + L        + +N +KL++N+L++        T  AAV A  SS+ R  
Sbjct: 154 GFYELGVPDYVL------PLMSNNVTKLLSNTLSLNNMSGKYTTVKAAVDAAPSSSGRYV 213

Query: 183 ----------------SQRLLAGHNSSEKMLAAISAV--------------VGEGFIAKD 242
                           +  +L G    + ++    +V              VG+GFIA+D
Sbjct: 214 IYVKGGVYNEQVEVKANNIMLVGDGIGKTIITGSKSVGGGTTTFRSATVAAVGDGFIAQD 273

Query: 243 MTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECHIYGTIDFIF 298
           +TF N AGA  HQAV+ R+ +D S+FYRCSF+G+QDTLY HS R F+REC IYGT+DFIF
Sbjct: 274 ITFRNTAGAANHQAVAFRSGSDLSVFYRCSFEGFQDTLYVHSERQFYRECDIYGTVDFIF 333

[Close]
BLAST of Cp4.1LG13g03580 vs. TrEMBL
Match: A0A0K9NSC3_ZOSMR (Pectinesterase OS=Zostera marina GN=ZOSMA_72G00560 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 3.1e-44
Identity = 89/124 (71.77%), Postives = 102/124 (82.26%), Query Frame = 1 

Query: 185 AAISAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRH 244
           +A  AV G+GFIAK MTF+N AGA KHQAV++R+++D S+FYRCSFKGYQDTLY HS R 
Sbjct: 300 SATFAVAGKGFIAKGMTFQNTAGAMKHQAVAMRSDSDMSVFYRCSFKGYQDTLYVHSQRQ 359

Query: 245 FFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGRRLDSGH 304
           F+REC IYGT+DFIFGDG  V QNC I+VRKPM NQKNTVTAQGRSDPNENTG  +   H
Sbjct: 360 FYRECDIYGTVDFIFGDGVVVLQNCKIYVRKPMRNQKNTVTAQGRSDPNENTGISI---H 419

Query: 305 RSAI 309
           RS I
Sbjct: 420 RSVI 420

[Close]
BLAST of Cp4.1LG13g03580 vs. TrEMBL
Match: A0A072TV68_MEDTR (Pectinesterase OS=Medicago truncatula GN=MTR_8g104655 PE=4 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.2e-43
Identity = 84/120 (70.00%), Postives = 102/120 (85.00%), Query Frame = 1 

Query: 178 NSSEKMLAAISAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTL 237
           + S    +A   V+G+GFIAKDMTFEN AG +KHQAV+LR+ AD S+FYRC+FKG+QDTL
Sbjct: 301 DGSTTFRSATFGVMGDGFIAKDMTFENTAGPQKHQAVALRSGADHSVFYRCAFKGFQDTL 360

Query: 238 YAHSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 297
           Y ++NR F+R+C+IYGTIDFIFG+  +V QNCNIFVRKPM NQKNTVTAQGR+DPNENTG
Sbjct: 361 YVYANRQFYRDCNIYGTIDFIFGNAVTVLQNCNIFVRKPMSNQKNTVTAQGRTDPNENTG 420

[Close]
BLAST of Cp4.1LG13g03580 vs. TrEMBL
Match: A0A072TX14_MEDTR (Pectinesterase OS=Medicago truncatula GN=MTR_8g104645 PE=4 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.2e-43
Identity = 84/120 (70.00%), Postives = 103/120 (85.83%), Query Frame = 1 

Query: 178 NSSEKMLAAISAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTL 237
           + S    +A  AV+G+GFIAKDMTFEN AG +KHQAV+LR+ AD S+FYRC+FKG+QDTL
Sbjct: 132 DGSTTFRSATFAVMGDGFIAKDMTFENTAGPQKHQAVALRSGADHSVFYRCAFKGFQDTL 191

Query: 238 YAHSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 297
           Y ++NR F+R+C+IYGTIDFIFG+  +V QNCNIFVRKPM NQ+NTVTAQGR+DPNENTG
Sbjct: 192 YVYANRQFYRDCNIYGTIDFIFGNAVTVLQNCNIFVRKPMSNQQNTVTAQGRTDPNENTG 251

[Close]
BLAST of Cp4.1LG13g03580 vs. TAIR10
Match: AT5G04970.1 (AT5G04970.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 171.8 bits (434), Expect = 6.9e-43
Identity = 78/109 (71.56%), Postives = 91/109 (83.49%), Query Frame = 1 

Query: 189 AVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRE 248
           AVVG+ F+A D+TF N AG EKHQAV++R NAD S FYRCSF+GYQDTLY HS R F+RE
Sbjct: 392 AVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQDTLYVHSLRQFYRE 451

Query: 249 CHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 298
           C IYGTIDFIFG+ A++FQNCNI+ RKPM NQKN VTA GR+DPN+ TG
Sbjct: 452 CDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQKTG 500

[Close]
BLAST of Cp4.1LG13g03580 vs. TAIR10
Match: AT3G10720.2 (AT3G10720.2 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 169.5 bits (428), Expect = 3.4e-42
Identity = 77/109 (70.64%), Postives = 90/109 (82.57%), Query Frame = 1 

Query: 189 AVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRE 248
           AVVGE F+A D+TF N AG EKHQAV+LR NA+ S FYRCSF+GYQDTLY HS R F+RE
Sbjct: 387 AVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLYVHSLRQFYRE 446

Query: 249 CHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 298
           C IYGT+DFIFG+ A++FQNCNI+ RKPM  QKN +TA GR DPN+NTG
Sbjct: 447 CDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTG 495

[Close]
BLAST of Cp4.1LG13g03580 vs. TAIR10
Match: AT3G60730.1 (AT3G60730.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 169.1 bits (427), Expect = 4.5e-42
Identity = 76/113 (67.26%), Postives = 95/113 (84.07%), Query Frame = 1 

Query: 185 AAISAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRH 244
           +A   V G+GF A+D+TFEN AG  KHQAV+LR ++D S+FYRCSFKGYQDTL+ HS R 
Sbjct: 286 SATFGVSGDGFWARDITFENTAGPHKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQ 345

Query: 245 FFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 298
           F+R+CHIYGTIDFIFGD A+VFQNC+IFVR+PM++Q N +TAQGR DP+ N+G
Sbjct: 346 FYRDCHIYGTIDFIFGDAAAVFQNCDIFVRRPMDHQGNMITAQGRDDPHTNSG 398

[Close]
BLAST of Cp4.1LG13g03580 vs. TAIR10
Match: AT1G23200.1 (AT1G23200.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 164.9 bits (416), Expect = 8.4e-41
Identity = 74/113 (65.49%), Postives = 91/113 (80.53%), Query Frame = 1 

Query: 185 AAISAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRH 244
           +A  AV G GFIA+ +TFEN AG EKHQAV+LR+++D S+FY CSFKGYQDTLY HS+R 
Sbjct: 330 SATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQ 389

Query: 245 FFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 298
           F R C+IYGT+DFIFGD  ++ QNCNI+ RKPM  QKNT+TAQ R +P+E TG
Sbjct: 390 FLRNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTG 442

[Close]
BLAST of Cp4.1LG13g03580 vs. TAIR10
Match: AT3G49220.1 (AT3G49220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 163.7 bits (413), Expect = 1.9e-40
Identity = 74/109 (67.89%), Postives = 87/109 (79.82%), Query Frame = 1 

Query: 189 AVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRE 248
           A  G GFIA+D+TFEN AG  KHQAV+LR  AD ++ YRC+  GYQDTLY HSNR FFRE
Sbjct: 367 AATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRE 426

Query: 249 CHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 298
           C IYGT+DFIFG+ A V QNC+I+ RKPM+ QKNT+TAQ R DPN+NTG
Sbjct: 427 CDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTG 475

[Close]
BLAST of Cp4.1LG13g03580 vs. NCBI nr
Match: gi|1011992736|ref|XP_015961500.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 6 [Arachis duranensis])

HSP 1 Score: 260.8 bits (665), Expect = 3.2e-66
Identity = 159/337 (47.18%), Postives = 206/337 (61.13%), Query Frame = 1 

Query: 1   MSCSQTPYPDLCFHFINPNHNVPTTDVHQSYNITRFRNSAIRATLNQAMVAHHHLLSAMD 60
           +SC+QTPYP LC H+I     + TT+   S +   F + A++ T++QA+VAH  + S M+
Sbjct: 28  LSCNQTPYPSLCNHYI-----IGTTNYSISSSYYSFHDMALKVTMDQAIVAHKLVSSTME 87

Query: 61  LASFDDDDRVKSALIDCLELYEDSITELNRSVMNSMRANSIDHLTLLSASLANHQTCRDG 120
              F D  R KSA  DCLELYE++I +LNRS+ ++   N  D LT  SAS+AN QTC++G
Sbjct: 88  SNHFKDK-RAKSAWEDCLELYENTIYQLNRSMSSN---NLNDRLTWQSASIANQQTCQNG 147

Query: 121 FRDFGFSSHYLNNIIFPHQILSNFSKLITNSLAITKAAAVTATTSSNP--RSQRLLAGHN 180
           F D   SSHYLN   FP  I +N S L++NSLAIT       +   +P  R  R L   N
Sbjct: 148 FLDSNLSSHYLN--YFP-SISTNLSNLLSNSLAITNTLTSFTSLPKHPVARGGRKLLSFN 207

Query: 181 --------SSEKML------AAISA------------------------VVGEGFIAKDM 240
                   S  ++L      AA  A                        V  +GFIA+D+
Sbjct: 208 GFPEWLSPSDRRLLQDLPGTAAPKADIVVAQDGSGNYKSVSEGVVIRETVSSDGFIARDL 267

Query: 241 TFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECHIYGTIDFIFG 298
           T EN AG +KHQAV+L +++D S+FYRCSF+GYQDTLY  S R F+R+C IYGTIDFIFG
Sbjct: 268 TIENSAGPQKHQAVALLSSSDHSVFYRCSFRGYQDTLYVLSQRQFYRDCDIYGTIDFIFG 327

[Close]
BLAST of Cp4.1LG13g03580 vs. NCBI nr
Match: gi|659072831|ref|XP_008467117.1| (PREDICTED: pectinesterase [Cucumis melo])

HSP 1 Score: 223.8 bits (569), Expect = 4.3e-55
Identity = 118/176 (67.05%), Postives = 134/176 (76.14%), Query Frame = 1 

Query: 1   MSCSQTPYPDLCFHFINPNHNVPTTDVHQSYNITRFRNSAIRATLNQAMVAHHHLLSAMD 60
           MSC QTPYPDLCFH+INPN N+PT D+ ++Y  TRFRNSA++ATLNQA   H HL S MD
Sbjct: 25  MSCFQTPYPDLCFHYINPNDNIPTADIDETYLNTRFRNSAVQATLNQARKVHDHLRSIMD 84

Query: 61  LASFDDDDRVKSALIDCLELYEDSITELNRSVMNSMRANSIDHLTLLSASLANHQTCRDG 120
           L SFDDD+R K ALIDCLE YEDSI +LNRSV +S   NSIDHLT+LSASLANHQTC DG
Sbjct: 85  LGSFDDDNRNKVALIDCLEFYEDSIAKLNRSVFSSTSVNSIDHLTMLSASLANHQTCLDG 144

Query: 121 FRDFGFSSHYLNNIIFPHQILSNFSKLITNSLAITKAAAVTATTSSNPRSQRLLAG 177
           FRDF  S    NN   P QI+SNFSKL++NSLAITKAA   A++SS    Q L  G
Sbjct: 145 FRDFDNS----NNFFLPIQIMSNFSKLVSNSLAITKAAVAPASSSSTSCGQPLPDG 196

[Close]
BLAST of Cp4.1LG13g03580 vs. NCBI nr
Match: gi|449464802|ref|XP_004150118.1| (PREDICTED: pectinesterase [Cucumis sativus])

HSP 1 Score: 223.4 bits (568), Expect = 5.7e-55
Identity = 120/177 (67.80%), Postives = 135/177 (76.27%), Query Frame = 1 

Query: 1   MSCSQTPYPDLCFHFINPNHNVPTTDVHQSYNITRFRNSAIRATLNQAMVAHHHLLSAMD 60
           MSCSQTPYPDLCFH+INPN N+ T  + ++Y ITRFRNSAI+ATL QAM    HL S MD
Sbjct: 25  MSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQATLYQAMKVRDHLRSTMD 84

Query: 61  LASFDDDDRVKSALIDCLELYEDSITELNRSVMNSMRANSIDHLTLLSASLANHQTCRDG 120
           L SFDD+DR   ALIDCLE YEDSI ELNRS ++S  ANSIDH T+LSASLANHQTC DG
Sbjct: 85  LGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSANSIDHSTMLSASLANHQTCLDG 144

Query: 121 FRDFGFSSHYLNNIIFPHQILSNFSKLITNSLAITKA-AAVTATTSSNPRSQRLLAG 177
           FRDFGF     NN   P QI+SNFSKL++NSLAITKA AA  +T+SS    Q LL G
Sbjct: 145 FRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAAAPSTSSSTSCGQPLLDG 201

[Close]
BLAST of Cp4.1LG13g03580 vs. NCBI nr
Match: gi|747049362|ref|XP_011070723.1| (PREDICTED: LOW QUALITY PROTEIN: pectinesterase [Sesamum indicum])

HSP 1 Score: 189.5 bits (480), Expect = 9.1e-45
Identity = 89/127 (70.08%), Postives = 104/127 (81.89%), Query Frame = 1 

Query: 178 NSSEKMLAAISAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTL 237
           + S    +A  A+ G+GFIA+DMTFEN AGA KHQAV+LR+++D SIFYRCSFKGYQDTL
Sbjct: 272 DGSTTFRSATFAISGDGFIARDMTFENTAGAHKHQAVALRSSSDFSIFYRCSFKGYQDTL 331

Query: 238 YAHSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTG 297
           Y HS R F+R+C IYGTIDFIFGD  +V QNCNI+VRKPM NQKNTVTAQGRSDPNENTG
Sbjct: 332 YVHSQRQFYRDCDIYGTIDFIFGDAVAVLQNCNIYVRKPMPNQKNTVTAQGRSDPNENTG 391

Query: 298 RRLDSGH 305
             + + H
Sbjct: 392 IIIHNSH 398

[Close]
BLAST of Cp4.1LG13g03580 vs. NCBI nr
Match: gi|901796077|gb|KMZ58895.1| (pectin methylesterase, family CE8 [Zostera marina])

HSP 1 Score: 187.2 bits (474), Expect = 4.5e-44
Identity = 89/124 (71.77%), Postives = 102/124 (82.26%), Query Frame = 1 

Query: 185 AAISAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHSNRH 244
           +A  AV G+GFIAK MTF+N AGA KHQAV++R+++D S+FYRCSFKGYQDTLY HS R 
Sbjct: 300 SATFAVAGKGFIAKGMTFQNTAGAMKHQAVAMRSDSDMSVFYRCSFKGYQDTLYVHSQRQ 359

Query: 245 FFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGRRLDSGH 304
           F+REC IYGT+DFIFGDG  V QNC I+VRKPM NQKNTVTAQGRSDPNENTG  +   H
Sbjct: 360 FYRECDIYGTVDFIFGDGVVVLQNCKIYVRKPMRNQKNTVTAQGRSDPNENTGISI---H 419

Query: 305 RSAI 309
           RS I
Sbjct: 420 RSVI 420

The following BLAST results are available for this feature:
BLAST of Cp4.1LG13g03580 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PME47_ARATH1.2e-4171.56Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana GN=P... [more]
PME25_ARATH6.1e-4170.64Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=P... [more]
PME36_ARATH7.9e-4167.26Probable pectinesterase/pectinesterase inhibitor 36 OS=Arabidopsis thaliana GN=P... [more]
PME6_ARATH1.5e-3965.49Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana GN=PM... [more]
PME34_ARATH3.3e-3967.89Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=P... [more]
BLAST of Cp4.1LG13g03580 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KUU2_CUCSA3.9e-5567.80Pectinesterase OS=Cucumis sativus GN=Csa_5G568300 PE=4 SV=1[more]
I1JKS0_SOYBN4.4e-4637.57Pectinesterase OS=Glycine max PE=4 SV=1[more]
A0A0K9NSC3_ZOSMR3.1e-4471.77Pectinesterase OS=Zostera marina GN=ZOSMA_72G00560 PE=4 SV=1[more]
A0A072TV68_MEDTR1.2e-4370.00Pectinesterase OS=Medicago truncatula GN=MTR_8g104655 PE=4 SV=1[more]
A0A072TX14_MEDTR1.2e-4370.00Pectinesterase OS=Medicago truncatula GN=MTR_8g104645 PE=4 SV=1[more]
BLAST of Cp4.1LG13g03580 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G04970.16.9e-4371.56 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G10720.23.4e-4270.64 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G60730.14.5e-4267.26 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT1G23200.18.4e-4165.49 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G49220.11.9e-4067.89 Plant invertase/pectin methylesterase inhibitor superfamily[more]
BLAST of Cp4.1LG13g03580 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|1011992736|ref|XP_015961500.1|3.2e-6647.18PREDICTED: probable pectinesterase/pectinesterase inhibitor 6 [Arachis duranensi... [more]
gi|659072831|ref|XP_008467117.1|4.3e-5567.05PREDICTED: pectinesterase [Cucumis melo][more]
gi|449464802|ref|XP_004150118.1|5.7e-5567.80PREDICTED: pectinesterase [Cucumis sativus][more]
gi|747049362|ref|XP_011070723.1|9.1e-4570.08PREDICTED: LOW QUALITY PROTEIN: pectinesterase [Sesamum indicum][more]
gi|901796077|gb|KMZ58895.1|4.5e-4471.77pectin methylesterase, family CE8 [Zostera marina][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR006501Pectinesterase_inhib_dom
IPR000070Pectinesterase_cat
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g03580.1Cp4.1LG13g03580.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 181..301
score: 4.9
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 2..156
score: 4.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 2..154
score: 5.8
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 1..154
score: 7.9
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 2..156
score: 8.3
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 2..156
score: 3.92
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 179..303
score: 7.06
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 179..297
score: 9.4
NoneNo IPR availableunknownCoilCoilcoord: 318..320
scor
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 1..297
score: 1.5E
NoneNo IPR availablePANTHERPTHR31707:SF27PECTINESTERASE/PECTINESTERASE INHIBITOR 6-RELATEDcoord: 1..297
score: 1.5E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG13g03580CmaCh15G009660Cucurbita maxima (Rimu)cmacpeB305
Cp4.1LG13g03580CmoCh15G010080Cucurbita moschata (Rifu)cmocpeB268
Cp4.1LG13g03580MELO3C005292.2Melon (DHL92) v3.6.1cpemedB176
Cp4.1LG13g03580MELO3C012316.2Melon (DHL92) v3.6.1cpemedB187
Cp4.1LG13g03580CsaV3_5G035040Cucumber (Chinese Long) v3cpecucB0228
Cp4.1LG13g03580CsaV3_5G028290Cucumber (Chinese Long) v3cpecucB0212
Cp4.1LG13g03580Bhi12G000797Wax gourdcpewgoB0227
Cp4.1LG13g03580CsGy5G018950Cucumber (Gy14) v2cgybcpeB600
Cp4.1LG13g03580Carg19014Silver-seed gourdcarcpeB1465
Cp4.1LG13g03580Carg01884Silver-seed gourdcarcpeB1175
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG13g03580Cucurbita pepo (Zucchini)cpecpeB200
Cp4.1LG13g03580Cucumber (Gy14) v1cgycpeB0735
Cp4.1LG13g03580Cucurbita maxima (Rimu)cmacpeB706
Cp4.1LG13g03580Cucurbita moschata (Rifu)cmocpeB658
Cp4.1LG13g03580Wild cucumber (PI 183967)cpecpiB179
Cp4.1LG13g03580Cucumber (Chinese Long) v2cpecuB177
Cp4.1LG13g03580Bottle gourd (USVL1VR-Ls)cpelsiB135
Cp4.1LG13g03580Watermelon (Charleston Gray)cpewcgB169
Cp4.1LG13g03580Watermelon (97103) v1cpewmB188
Cp4.1LG13g03580Melon (DHL92) v3.5.1cpemeB149
Cp4.1LG13g03580Silver-seed gourdcarcpeB0599