CsGy4G002580 (gene) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy4G002580
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	DNA-directed RNA polymerases II and V subunit 8A-like
Location	Chr4 : 1634438 .. 1638437 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCTCAATGAATATAAACCCTAAACGTGAGTCATCGCCTTATACTCACCGAGTCACGCTGAACTTCCTCTTCAGCGAACGAGTCACGCCCCCCATCTTCTTCGACCAAGACCCTACGTTTGCCCTAACTCTTCGCCGCTCCTTGACGCCGTCGGTCAGTTTCAACCCTCTGTTTTTGTCCTCTCTCTCTCTACACACACACACACACACGTAGTTTATAATATCGAATTCGTTTCTGCTTTTATCGGGTTTTAGAGTGGCTTTGTAATCTGGTTGTTGATTTTTTTCTTATTCTTTCAATACAAACAATGTCGTTGATAGAATGTTAGCACTCCTAGTTCTTGGCTGCCTCTAATTCCATGGATAAGTATAGGTTAAAGTAAGTTTATGTGCAAGGCATTGTATATTTCCAAATGCACTTGTCAAGTTGATCTTCTTATTCAATTTTGCCTATATGGGCTGCTGGGTTTCTTATCTGTCTGTCGATGTTGGAGTTGGCTTTCCCTCCCTTGGTTAGTTTCCCATTTTTTGTCCTCATCACATGGCTGTTTTTTCTCATAGTTTCAGACGCTAAGGTGTTTGGAGTTTCGTTCCAAGCCATTTTATTCCGGGTTGTATTTGCTTTTATTTTCTTGTTTAGTTCAGTTATGGGCTATATTTGGTTGTATTTTTGTTATTTTGCTTTCATTTCATCGAAAAGCTCTTTTTTTTTTTAAAAAAAATAATAATTTGGGAGGGCCGTAAGCTAACCCTGTTAATTTTCTTGCATACGAAAACCTTGTATATCCTTTCTCGTACTTAATTTGAATCTGCAGAACCCATGTCAGATATTTTACTTAATATATTGATGTGTTCTTGCTTTGAAATATTTGTTCTTTAATTCCTGTATTCACTCCTCGATATGAATGTAAAATCGTTTACGCACGGACTTCATCCACTGCTTGACTATAACAAATGTCTTTTCACTCCACTTCTCTATTTGATGTGAATCTCCAACCTGGCTCTAACTCAGTTATGATGAGTGCTTGTCACAGAGATGTCAATGCTACGATTGAGGAGTCCTTCCTCAATCTGTCTTTTGGAAAGAAAGCCGTTTCTTTGGCCTGCCGACGTGTGTGCGGTGTTATGAGTCTTGTGGGATGAGAGGAACAGTAAGATGTTTAGGGTTGTGGAGAGGGACCCTAGGAAGCTTTGGGTCCCTTGTTCATTTTCATATTTCTCTTTGGGCTTCAACTTCAAAGACTTTTTGTAATTTTTCTGTAGGAGTGGTTTTGCATAGTTTAAACAGTCCTTGTAAAGGGTACCCTGCCTTTCTGTTGACTTGGTTTTTTTGTATGTCCGTGCATTCTTTCATTTTTTCTCACTGAAAGTGGTTATTTTCATTTAAAAACCCAGTGGGTTGCCTCCCCATTTACACCGAATGTTTAACACAGTTCTTATTTTTCAATCTTTTGCTTTGAAACCTGGCTTCCATTCACTAATGCGGTTCTTTTTTTGGCTGGTCTGGTCTGTTCTCTGATGGTCATACATTTTCTAGCACTGCTGGTTAATTTCTATTATTGCCCTGTTAATTTACAGGTTAGCGTTAACTTTTTGACAACAAAACTGCTGTTGAAGGATGGTTGAGCTTCTGTTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGGTACATTTTTGTATAGTAGTCACTTTCATTTCATTTATACAATATTTCCAGGAATGCTTATTAGCAGACTGTGGCAGTTTTATTGTTATATATCTGTAAAATCATTGTGCCATTCTTTAACTTTCTGATGGCGGTTGGAGATGTACTTCGTAGGCCTTTAAATCTCATCCATTCCTTATTTATTAACTTTTTAGAGGTTTCCTTTCAATCAGTTAGGATATCATCTGGAATATTATAACTCCATGTAAAATATGTGTTAAAAAAACTTTTCAACATATTTATGACATATTTTTTCACAATCTGTCCAACAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATATAAATTCAGAGATATACCCCCTGAAAGAAGGTGAGAAGTTCTCGATGGCACTAGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGTAACTTGTTCTTCTATTGATTTTAATCTGTTGCACACTGGATTTAACGTGGTGGTGTTGGTAAGAAAATGATGTCCTGGTAGGTTGAAGGATTTAGGCCTTGTTTGGCAACTTTTTAGGCCCTGTTTAGTAATCATTTGGTTTTTAGTTGAAAATTATGACTATAGACTTCAATTCCACATCTAAATTTATTGCTGTGTTATCTGCATTTTACAAGTATTTTTAAAAAGTGAAGCCAAAATTTGAAAACATGGAAAAGTAGTTTTTAAAAACTAAGAAATTGAGGGGAAAAGTTTAATTTTCAAAACTCAAAAACAAAAAGCGAAATAGAGTCTGAGTTTTTAGTTATTTGTTTTTGAAAATCTTATAAATATCCCTTTGTCCTCTAAAAAATTTGTTATCTACATTAAAACCAAGCGTAAATTTGAGAACTAAAAAATGTAGTTTTAAAAAACTTGTTTTACTTTTTAGAATTTGATTAAGAATTCAACTCTTAAGAAAAATATAAACCATTGCAAGAAATTGAGAGGAAGTAAGCTTAATCTTAAGAAACAAAAACTGAATAGTTACCAAATGGGGTCTTAGTAGTCCATTTTGTCATAAACTACTATATTTGAAATATCACTTTGAACATTAATAGGGAACAAGAAACTTTCAATCTGCTAGGAAATATATGCTTTCCATAGTTATTGCATATGTCAAGACTGGTCTTCATTGTTTTCCGACAACTATATTTGGTCTTGAGCATTTTCGACACTTGAATATGTCTACTTAACAATCATTAGGCTGCATAACAGGGCAATCGGAAGTCTCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGCGGAAAAGCGTACGTGCTATAACTTCTCGAAACAATTTTAATGTGACACTTTAATTCCACTGTATGAGCATTAGAGAACCGAGAGCTTGGCATTGCCAAACTATACTTATCTAAATTTGAGCAAGAACCTGTCACGGTGCATTCTCCTGTCTATAATGGCTACAGAAGTGTATTGCGTGTGAATAAAATTCATGTTAGTTTCTTTTCCTGAACTGTAGGAACATATATATATTTATTCTAGTAGAATTGTTATCTAAAAAACTATATTTCATCTGAATCTAGTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGACCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGAAGCGCTTTCATTTGATTTGAACTCTAATGACTTGCTACAAAAGCTCTGGACATCTCTTATCGCTATTAGAACTGTGAATGTTTAGAAATATGTATTGTTAGAATCAAATTTCTATCTGTATCTGATTAAAGACTGGGCAGTGATCTTATCTTCTGAACTAAATTGATATCTTGCCTATAATGTCATGAATAGTGGCAGAACCTTTCGAGTTCTCGAAAGCGGAGTTTGAGCATGGTAGTATGTATAGTTTGTAGTGTTGGGCGGCCAACCTGAACAATTTTGGCAAGCTTGATTCCATTGCAGTTTGGGAGGGTGGAGACTGGCTAATGAAAGATTTAATAAATTACAAACATATTTAAGTGGGTGTGAGATTTGAATTTCTCACCTCAATGTTGCTATTAAACGTCTTTGAGATTTATTTCGTTGGTCTCTAGATTGTCATTATTTTTGTGGATGGAAAGTATATTTTCTTTATATGTACATGTATGTATTAAGTAATGATAAATAAATTTAGTTAGTTATTAATGTAACCATAATAAACACTTGAAACACCAATAATTTTTCTTT

mRNA sequence

CGCTCAATGAATATAAACCCTAAACGTGAGTCATCGCCTTATACTCACCGAGTCACGCTGAACTTCCTCTTCAGCGAACGAGTCACGCCCCCCATCTTCTTCGACCAAGACCCTACGTTTGCCCTAACTCTTCGCCGCTCCTTGACGCCGTCGGTTAGCGTTAACTTTTTGACAACAAAACTGCTGTTGAAGGATGGTTGAGCTTCTGTTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATATAAATTCAGAGATATACCCCCTGAAAGAAGGTGAGAAGTTCTCGATGGCACTAGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGGCAATCGGAAGTCTCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGCGGAAAAGCTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGACCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGAAGCGCTTTCATTTGATTTGAACTCTAATGACTTGCTACAAAAGCTCTGGACATCTCTTATCGCTATTAGAACTGTGAATGTTTAGAAATATGTATTGTTAGAATCAAATTTCTATCTGTATCTGATTAAAGACTGGGCAGTGATCTTATCTTCTGAACTAAATTGATATCTTGCCTATAATGTCATGAATAGTGGCAGAACCTTTCGAGTTCTCGAAAGCGGAGTTTGAGCATGGTAGTATGTATAGTTTGTAGTGTTGGGCGGCCAACCTGAACAATTTTGGCAAGCTTGATTCCATTGCAGTTTGGGAGGGTGGAGACTGGCTAATGAAAGATTTAATAAATTACAAACATATTTAAGTGGGTGTGAGATTTGAATTTCTCACCTCAATGTTGCTATTAAACGTCTTTGAGATTTATTTCGTTGGTCTCTAGATTGTCATTATTTTTGTGGATGGAAAGTATATTTTCTTTATATGTACATGTATGTATTAAGTAATGATAAATAAATTTAGTTAGTTATTAATGTAACCATAATAAACACTTGAAACACCAATAATTTTTCTTT

Coding sequence (CDS)

ATGGTTGAGCTTCTGTTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATATAAATTCAGAGATATACCCCCTGAAAGAAGGTGAGAAGTTCTCGATGGCACTAGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGGCAATCGGAAGTCTCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGCGGAAAAGCTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGACCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGA

Protein sequence

MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKGDPSHCSKFELDQRLFLLIRKV

Homology

[Close]

BLAST of CsGy4G002580 vs. NCBI nr
Match: XP_004152137.1 (PREDICTED: DNA-directed RNA polymerases II and V subunit 8A [Cucumis sativus] >KGN53006.1 hypothetical protein Csa_4G011050 [Cucumis sativus])

HSP 1 Score: 242.7 bits (618), Expect = 7.9e-61
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CsGy4G002580 vs. NCBI nr
Match: XP_008454076.1 (PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo])

HSP 1 Score: 240.4 bits (612), Expect = 3.9e-60
Identity = 143/144 (99.31%), Postives = 143/144 (99.31%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFD XXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDXXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CsGy4G002580 vs. NCBI nr
Match: XP_022932746.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_022932747.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_023538873.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita pepo subsp. pepo] >XP_023538874.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 237.3 bits (604), Expect = 3.3e-59
Identity = 133/144 (92.36%), Postives = 133/144 (92.36%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDK  XXXXXXXXXXXXX       IY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTXXXXXXXXXXXXXHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVM GKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CsGy4G002580 vs. NCBI nr
Match: XP_022145813.1 (DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 [Momordica charantia] >XP_022145814.1 DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 [Momordica charantia] >XP_022145815.1 DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 [Momordica charantia])

HSP 1 Score: 236.5 bits (602), Expect = 5.7e-59
Identity = 131/144 (90.97%), Postives = 134/144 (93.06%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDK  XXXXXXXXXXXXX       IY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTXXXXXXXXXXXXXHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGY+LQGNRKSLADRYEY+MQGKLFRISESSGHGG+AEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYIMQGKLFRISESSGHGGRAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CsGy4G002580 vs. NCBI nr
Match: XP_022972118.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita maxima] >XP_022972119.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita maxima])

HSP 1 Score: 233.8 bits (595), Expect = 3.7e-58
Identity = 132/144 (91.67%), Postives = 132/144 (91.67%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDK  XXXXXXXXXXXXX       IY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTXXXXXXXXXXXXXHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVM GKLFRISESSGH GKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHVGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CsGy4G002580 vs. TAIR10
Match: AT1G54250.1 (RNA polymerase Rpb8)

HSP 1 Score: 173.3 bits (438), Expect = 1.1e-43
Identity = 82/141 (58.16%), Postives = 103/141 (73.05%), Query Frame = 0


Query: 4   LLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMALAPT 63
           +LF+DIF +++L+PDGKKFDK                      +YPL  G+KF++ALAPT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+LKG
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLKG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of CsGy4G002580 vs. TAIR10
Match: AT3G59600.1 (RNA polymerase Rpb8)

HSP 1 Score: 170.6 bits (431), Expect = 6.9e-43
Identity = 79/141 (56.03%), Postives = 103/141 (73.05%), Query Frame = 0


Query: 4   LLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMALAPT 63
           ++F+DIF +++L+PDGKKFDK                      +YPL  G+KF++A+APT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+L+G
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLQG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of CsGy4G002580 vs. Swiss-Prot
Match: sp|O81097|RPB8A_ARATH (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 1.9e-42
Identity = 82/141 (58.16%), Postives = 103/141 (73.05%), Query Frame = 0


Query: 4   LLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMALAPT 63
           +LF+DIF +++L+PDGKKFDK                      +YPL  G+KF++ALAPT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+LKG
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLKG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of CsGy4G002580 vs. Swiss-Prot
Match: sp|Q9M1A8|RPB8B_ARATH (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.2e-41
Identity = 79/141 (56.03%), Postives = 103/141 (73.05%), Query Frame = 0


Query: 4   LLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMALAPT 63
           ++F+DIF +++L+PDGKKFDK                      +YPL  G+KF++A+APT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+L+G
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLQG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of CsGy4G002580 vs. Swiss-Prot
Match: sp|Q54YW8|RPAB3_DICDI (DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium discoideum OX=44689 GN=polr2h PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.1e-25
Identity = 59/143 (41.26%), Postives = 85/143 (59.44%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           M  +LF+D F+++ ++PDGKKFD+                      IYPL   E+F M L
Sbjct: 1   MTSILFEDTFEVKEIDPDGKKFDRVSRFVCYSENYEMDLQIDIATHIYPLGH-ERFKMVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           A +LNLDG+ D       N+ SLAD+Y+YVM GK+F+  + +    K  + ASFGGLL++
Sbjct: 61  ASSLNLDGSSDNSGSYDTNKPSLADKYDYVMYGKIFKYQKENS-SSKVSVFASFGGLLVL 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRK 144
           L+GDP +    ELD R++LLI+K
Sbjct: 121 LQGDPRYLPGIELDARIYLLIKK 141

[Close]

BLAST of CsGy4G002580 vs. Swiss-Prot
Match: sp|A8XT81|RPAB3_CAEBR (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae OX=6238 GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 2.9e-22
Identity = 56/145 (38.62%), Postives = 80/145 (55.17%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           M  ++FDD+F ++ ++PDGKKFD+                      IYPLK+ +KF + L
Sbjct: 1   MAGIIFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRI--SESSGHGGKAEIDASFGGLL 120
           A TL  DG  D G +          +YEYVM GK++R+   +S   GG+    ASFGGLL
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLL 120

Query: 121 MMLKGDPSHCSKFELDQRLFLLIRK 144
           M LKG+  +   FE+D  L+LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]

BLAST of CsGy4G002580 vs. Swiss-Prot
Match: sp|Q19826|RPAB3_CAEEL (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 3.8e-22
Identity = 56/145 (38.62%), Postives = 80/145 (55.17%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           M  ++FDD+FK++ ++PDGKKFD+                      IYPLK+ +K  + L
Sbjct: 1   MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRI--SESSGHGGKAEIDASFGGLL 120
           A TL  DG  D G +          +YEYVM GK++R+   ++   GGK    ASFGGLL
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLL 120

Query: 121 MMLKGDPSHCSKFELDQRLFLLIRK 144
           M LKG+  +   FE+D  L+LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]

BLAST of CsGy4G002580 vs. TrEMBL
Match: tr|A0A0A0KYV5|A0A0A0KYV5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011050 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 5.2e-61
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CsGy4G002580 vs. TrEMBL
Match: tr|A0A1S3BXS2|A0A1S3BXS2_CUCME (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN=LOC103494598 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 2.6e-60
Identity = 143/144 (99.31%), Postives = 143/144 (99.31%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFD XXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDXXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CsGy4G002580 vs. TrEMBL
Match: tr|W9RPZ0|W9RPZ0_9ROSA (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Morus notabilis OX=981085 GN=L484_007786 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 7.8e-49
Identity = 99/144 (68.75%), Postives = 110/144 (76.39%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           M E LF+DIFKI+RLNPDGKKFDK                      +YP+ EGEKFSMAL
Sbjct: 1   MAEFLFEDIFKIDRLNPDGKKFDKVTRIEAKSEKFDMFMHLDVGTDVYPMNEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           + TLN DGTPDTGY+  GNRK+LAD YEYVMQGKL+RI+E SGH GKAEIDASFGGLLMM
Sbjct: 61  SSTLNEDGTPDTGYYTPGNRKTLADNYEYVMQGKLYRIAEGSGHHGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPS+  K+ELDQRLFLLIRKV
Sbjct: 121 LKGDPSYFKKYELDQRLFLLIRKV 144

[Close]

BLAST of CsGy4G002580 vs. TrEMBL
Match: tr|A0A2P5AWG8|A0A2P5AWG8_9ROSA (RNA polymerase, Rpb OS=Trema orientalis OX=63057 GN=TorRG33x02_339420 PE=3 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 7.3e-47
Identity = 98/144 (68.06%), Postives = 110/144 (76.39%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           M E LF+D+FKI++LNPDGKKFDK                      +YPL+EGEKFSMAL
Sbjct: 1   MAEFLFEDVFKIDQLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNTEVYPLREGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           + TLN DGTPDTGYF  GNRK+LAD YEYVMQGKL+RISE SG  GKAEI+ASFGGLLMM
Sbjct: 61  SSTLNEDGTPDTGYFTPGNRKTLADNYEYVMQGKLYRISEGSGQ-GKAEINASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPS+  K+ELDQRLFLLIRKV
Sbjct: 121 LKGDPSYFKKYELDQRLFLLIRKV 143

[Close]

BLAST of CsGy4G002580 vs. TrEMBL
Match: tr|A0A2P5BC85|A0A2P5BC85_PARAD (RNA polymerase, Rpb OS=Parasponia andersonii OX=3476 GN=PanWU01x14_252030 PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.2e-46
Identity = 114/144 (79.17%), Postives = 125/144 (86.81%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKXXXXXXXXXXXXXXXXXXXXXXIYPLKEGEKFSMAL 60
           M E LF+D+FKI+RLNPDGKKFDK  XXXXXXXXXXXXXXXX    +YPL+EGEKFSMAL
Sbjct: 1   MAEFLFEDVFKIDRLNPDGKKFDKVTXXXXXXXXXXXXXXXXVNTEVYPLREGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           + TLN DGTPDT YF  GNRK+LAD YEYVMQGKL+RISE SG  GKAEI+ASFGGLLMM
Sbjct: 61  SSTLNEDGTPDTSYFTPGNRKTLADNYEYVMQGKLYRISEGSGQ-GKAEINASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPS+  K+ELDQRLFLLIRKV
Sbjct: 121 LKGDPSYFKKYELDQRLFLLIRKV 143

The following BLAST results are available for this feature:

BLAST of CsGy4G002580 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152137.1	7.9e-61	100.00	PREDICTED: DNA-directed RNA polymerases II and V subunit 8A [Cucumis sativus] >K...	[more]
XP_008454076.1	3.9e-60	99.31	PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo]	[more]
XP_022932746.1	3.3e-59	92.36	DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_0...	[more]
XP_022145813.1	5.7e-59	90.97	DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 [Momordica char...	[more]
XP_022972118.1	3.7e-58	91.67	DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita maxima] >XP_022...	[more]

BLAST of CsGy4G002580 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G54250.1	1.1e-43	58.16	RNA polymerase Rpb8	[more]
AT3G59600.1	6.9e-43	56.03	RNA polymerase Rpb8	[more]

BLAST of CsGy4G002580 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O81097\|RPB8A_ARATH	1.9e-42	58.16	DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702...	[more]
sp\|Q9M1A8\|RPB8B_ARATH	1.2e-41	56.03	DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=...	[more]
sp\|Q54YW8\|RPAB3_DICDI	2.1e-25	41.26	DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium disc...	[more]
sp\|A8XT81\|RPAB3_CAEBR	2.9e-22	38.62	Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab...	[more]
sp\|Q19826\|RPAB3_CAEEL	3.8e-22	38.62	Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab...	[more]

BLAST of CsGy4G002580 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KYV5\|A0A0A0KYV5_CUCSA	5.2e-61	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011050 PE=3 SV=1	[more]
tr\|A0A1S3BXS2\|A0A1S3BXS2_CUCME	2.6e-60	99.31	DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN...	[more]
tr\|W9RPZ0\|W9RPZ0_9ROSA	7.8e-49	68.75	DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Morus notabilis OX...	[more]
tr\|A0A2P5AWG8\|A0A2P5AWG8_9ROSA	7.3e-47	68.06	RNA polymerase, Rpb OS=Trema orientalis OX=63057 GN=TorRG33x02_339420 PE=3 SV=1	[more]
tr\|A0A2P5BC85\|A0A2P5BC85_PARAD	1.2e-46	79.17	RNA polymerase, Rpb OS=Parasponia andersonii OX=3476 GN=PanWU01x14_252030 PE=3 S...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0006351	transcription, DNA-templated

Vocabulary: **INTERPRO**
Term	Definition
IPR012340	NA-bd_OB-fold
IPR005570	RNA_pol_Rpb8

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006383	transcription from RNA polymerase III promoter
biological_process	GO:0006366	transcription from RNA polymerase II promoter
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	DNA-directed RNA polymerase I complex
cellular_component	GO:0005665	DNA-directed RNA polymerase II, core complex
cellular_component	GO:0005666	DNA-directed RNA polymerase III complex
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003899	DNA-directed RNA polymerase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy4G002580.1	CsGy4G002580.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005570	RNA polymerase, Rpb8	SMART	SM00658	rpol8neu	coord: 2..143 e-value: 3.7E-68 score: 242.4
IPR005570	RNA polymerase, Rpb8	PFAM	PF03870	RNA_pol_Rpb8	coord: 7..143 e-value: 1.9E-49 score: 167.3
IPR005570	RNA polymerase, Rpb8	PIRSF	PIRSF000779	RPB8	coord: 1..144 e-value: 6.9E-50 score: 166.8
IPR005570	RNA polymerase, Rpb8	PANTHER	PTHR10917	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3	coord: 1..144
None	No IPR available	GENE3D	G3DSA:2.40.50.140		coord: 6..144 e-value: 3.4E-52 score: 178.1
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	SSF50249	Nucleic acid-binding proteins	coord: 4..143

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsGy4G002580	Cla010901	Watermelon (97103) v1	cgybwmB291
CsGy4G002580	Csa4G011050	Cucumber (Chinese Long) v2	cgybcuB165
CsGy4G002580	MELO3C017775	Melon (DHL92) v3.5.1	cgybmeB269
CsGy4G002580	ClCG07G015110	Watermelon (Charleston Gray)	cgybwcgB298
CsGy4G002580	CSPI04G02730	Wild cucumber (PI 183967)	cgybcpiB168
CsGy4G002580	CmoCh06G016890	Cucurbita moschata (Rifu)	cgybcmoB535
CsGy4G002580	MELO3C017775.2	Melon (DHL92) v3.6.1	cgybmedB266
CsGy4G002580	CsaV3_4G002660	Cucumber (Chinese Long) v3	cgybcucB171
CsGy4G002580	Cla97C07G142080	Watermelon (97103) v2	cgybwmbB299
CsGy4G002580	Bhi01G002858	Wax gourd	cgybwgoB363
CsGy4G002580	Carg15437	Silver-seed gourd	carcgybB0693

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsGy4G002580	Cucurbita maxima (Rimu)	cgybcmaB485
CsGy4G002580	Cucurbita maxima (Rimu)	cgybcmaB560
CsGy4G002580	Cucurbita moschata (Rifu)	cgybcmoB455
CsGy4G002580	Cucurbita pepo (Zucchini)	cgybcpeB552
CsGy4G002580	Cucurbita pepo (Zucchini)	cgybcpeB575
CsGy4G002580	Bottle gourd (USVL1VR-Ls)	cgyblsiB285
CsGy4G002580	Melon (DHL92) v3.6.1	cgybmedB277
CsGy4G002580	Silver-seed gourd	carcgybB0127
CsGy4G002580	Wax gourd	cgybwgoB353