CSPI04G02730 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G02730
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	DNA-directed RNA polymerases I, II, and III subunit RPABC3
Location	Chr4 : 1652606 .. 1656499 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCATCGCCTTATACTCACTGAGTCACGCTGAACTTCCTCTTCAGCGAACGAGTCACGCCCCCCATCTTCTTCGACCAAGACCCTACGTTTGCCCTAACTCTTCGCCGCTCCTTGACGCCGTCGGTCAGTTTCAACCCTCTGTTTTTGTCCTCTCTCTCTCTATACACACACACACACACGTAGTTTATAATATCGAATTCGTTTCTGCTTTTATCGGGTTTTAGAGTGGCTTTGTAATCTGGTTGTTGATTTTTTTCTTATTCTTTCAATACAAACAATGTCGTTGATAGAATGTTAGCACTCCTAGTTCTTGGCTGCCTCTAATTCCATGGATAAGTATAGGTTAAAGTAAGTTTATGTGCAAGGCATTGTATATTTCCAAATGCACTTGTCGAGTTGATCTTCTTATTCAATTTTGCCTATATGGGCTGCTGGGTTTCTAATCTGTCTGTCGATGTTGGAGTTGGCCTTCCCTCCCTTGGTTAGTTTCCCATTTTTTGTCCTCATCACATGGCTGTTTTTTCTCATAGTTTCAGACGCTAAGGTGTTTGGAGTTTCGTTCCAAGCCATTTTATTCCGGGTTATATTTGCTTTTATTTTCTTGTTTAGTTCAGTTATGGGCTATATTTGGTTGTATTTTTGTTATTTTGCTTTCATTTAATCGAAAAGCTCTTTTTTCTTTAAAAAAAATAATAATTTGGGAGGGCCGTAAGCTAACCCTGTTAATTTTCTTGCATACGAAAACCTTGTATATCCTTTCTCGTACTTAATTTGAATCTGCAGAACCCATGTCAGATATTTTACTTAATATATTGATGTGTTCTTGCTTTGAAATATTTGTTCTTTAATTCCTGTATTCACTCCTCGATATGAATGTAAAATCGTTTACGCACGGACTTCATCCACTGCTTGACTATAACAAATGTCTTTTCACTCCACTTCTCTATTTGATGTGAATCTCCAACCTGGCTCTAACTCAGTTATGATGAGTGCTTGTCACAGAGATGTCAATGCTACGATTGAGGAGTCCTTCCTCAATCTGTCTTTGGAAAGAAAGCCGTTTCTTTGGCTTGCCGGCGTGTGTGCGGTGTTATGAGTCTTGTGGGATGAGAGGAACAGTAGGATGTTTAGGGTTGTGGAGAGGGACCCTAGGAAGCTTTGGGTCCCTTGTTCATTTTCATATTTCTCTTTGGGCTTCAACTTCAAAGACTTTTTGTAATTTTTCTGTAGGAATGGTTTTGCATAGTTTAAACAGTCCTTGTAAAGGGTACCCTGCCTTTCTGTTGACTTGGTTTTTTTGTATGTCCGTGCATTCTTTCATTTTTTCTCACTGAAAGTGGTTATTTTCATTTAAAAACCCAGTGGGTTGCCTCCCCATTTACACCGAATGTTTAACACAGTTCTTATTTTTCAATCTTTTGCTTTGAAACCCAATGCTTCCATTCACTAATGCGGTTCTTTTTTTGGCTGGTCTGGTCTGTTCTCTGATGGCCATACAGTTTCTAGCACTGCTGGTTAATTTCTATTATTGCCCTGTTAATTTACAGGTTAGCGTTAACTTTTTGACAACAAAACTGCTGTTGAAGGATGGTTGAGCTTCTGTTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGGTACATTTTTGTATAGTAGTCACTTTCATTTCATTTATACAATATTTCCAGGAATGCTTATTAGCAGACTGTGGCAGTTTTATTGTTATATATCTGTAAAATCATTGTGCCATTCTTTAACTTTCTGATGGCGGTTGGAGATGTACTTCGTAGGCCTTTAAATCTCATCCATTCCTTATTTATTAACTTTTTAGAGGTTTCCTTTCAATCAGTTAGGATATCATCTGGAATATTATAACTCCATGTAAAATATGTGTTAAAAAAAACTTTTCAACATATTTATGACATATTTTTTCACAATCTGTCCAACAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATATAAATTCAGAGATATACCCCCTGAAAGAAGGTGAGAAGTTCTCGATGGCACTAGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGTAACTTGTTCTTCTATTGATTTTAATCTGTTGCACACTGGATTTAACGTGGTGGTGTTGGTAAGAAAATGATGTCCTGGTAGGTTGAAGGATTTAGGCCTTGTTTGGCAACTTTTTAGGCCCTGTTTAGTAATCATTTGGTTTTTAGTTGAAAATTATGACTATAGACTTCAATTCCACATCTAAATTTATTGCTGTGTTATCTGCATTTTACAAGTATTTTTAAAAAGTGAAGCCAAAATTTGATACATTTTACAAATTTTAAAAAAGTGAAGCCAAAATTTGAAAACATGGAAAAGTAGTTTTTAAAAACTAAGAAATTGAGGGGAAAAGTTTAATTTTCAAAACTCAAAAACAAAAAACGAAATAGTCTGAGTTTTTGGTTTTTTGTTTTTGAAAATCTTATAAATATCCCTTTGTCCTCTAAAAAATTTGTTATCTACATTAAAACCAAGCGTAAATTTGAGAACTAAAAAATGTAGTTTTAAAAAACTTGTTTTACTTTTTAGAATTTGATTAAGAATTCAACTCTTAAGAAAAATATAAACCATTGCAAGAAATTGAGAGGAAGTAAGCTTAATCTTAAGAAACAAAAACCGAATAGTTACCAAATGGGGTCTTAGTAGTCCATTTTGTCATAAACTACTATATTTGAAATTTCACTTTGAACATTAATAGGGAACAAGAAACTTTCAATCTGCTAGGAAATATATGCTTTCCATAGTTATTGCATATGTCAAGACTGGTCTTCATTGTTTTCTGACAACTATATTTGGTCTTGAGCATTTTCGACACTTGAATATGTCTACTTAACAATCATTAGGCTGCATAACAGGGCAATCGAAAGTCTCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGCGGAAAAGCGTACGTGCTATAACTTCTCGAAACAATTTTAATGTGACTTTAATTCTACTGTATGAGCATTAGAGAACCGAGAGCTTGGCATTGCCAAACTATACTTATCTAAATTTGAGCAAGAACCTGTCACTGTGCATTCTCCTGTCTATAATGGCTAGAAGTGTATTGCGTGTGAATAAAATTCATGTTAGTTTCTTTTCCTGAACTGTAGGAACATATATATATTTATTCTAGTAGAATTGTTATCTAAAAATCTATATTTCATCTGAATCTAGTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGACCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGAAGCGCTTTCATTTGATTTGAACTCTAATGACTTGCTACAAAAGCTCTGGACATCTCTTATCGCTATTAGAACTGTGAATGTTTAGAAATATGTATTGTTAGAATCAAATTTCTATCTGTATCTGATTAAAGACTGGGCAGTGATCTTATCTTCTGAACTAAATTGATATCTTGCCTATAATGTCATGAATAGTGGCAGAACCTTTCGAGTTCTCGAAAGCGGAGTTTGAGCATGGTAGTATGTATAGTTTGTAGTGTTGGGCGGCCAACCTGAACAATTTTGGCAAGCTTGATTCCATTGCAGTTTGGGAGGGTGGAGACTGGCTAATGAAAGATTTAATAAATTACAAACATATTTAAGTGGGTGTGAGATTTGAATTTCTCACCTCAATGTTGCTATTAAACGTCTTTGAGATTTATTTCGTTGGTCTCTAGATTGTCATTATTTTTGTGGAT

mRNA sequence

ATGGTTGAGCTTCTGTTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATATAAATTCAGAGATATACCCCCTGAAAGAAGGTGAGAAGTTCTCGATGGCACTAGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGGCAATCGAAAGTCTCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGCGGAAAAGCTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGACCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGA

Coding sequence (CDS)

ATGGTTGAGCTTCTGTTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATATAAATTCAGAGATATACCCCCTGAAAGAAGGTGAGAAGTTCTCGATGGCACTAGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGGCAATCGAAAGTCTCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGCGGAAAAGCTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGACCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGA

Homology

[Close]

BLAST of CSPI04G02730 vs. Swiss-Prot
Match: RPB8A_ARATH (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 4.8e-54
Identity = 95/141 (67.38%), Postives = 121/141 (85.82%), Query Frame = 1


Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           +LF+DIF +++L+PDGKKFDKVTR++A S   +MFMHLD+N+E+YPL  G+KF++ALAPT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+LKG
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLKG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of CSPI04G02730 vs. Swiss-Prot
Match: RPB8B_ARATH (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 6.3e-54
Identity = 93/141 (65.96%), Postives = 122/141 (86.52%), Query Frame = 1


Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           ++F+DIF +++L+PDGKKFDKVTR+EA+S   +MFMHLD+N+E+YPL  G+KF++A+APT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+L+G
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLQG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of CSPI04G02730 vs. Swiss-Prot
Match: RPAB3_DICDI (DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium discoideum GN=polr2h PE=3 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 4.0e-32
Identity = 66/143 (46.15%), Postives = 98/143 (68.53%), Query Frame = 1


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           M  +LF+D F+++ ++PDGKKFD+V+R    SE ++M + +DI + IYPL   E+F M L
Sbjct: 1   MTSILFEDTFEVKEIDPDGKKFDRVSRFVCYSENYEMDLQIDIATHIYPLGH-ERFKMVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           A +LNLDG+ D       N+ SLAD+Y+YVM GK+F+  + +    K  + ASFGGLL++
Sbjct: 61  ASSLNLDGSSDNSGSYDTNKPSLADKYDYVMYGKIFKYQKENS-SSKVSVFASFGGLLVL 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRK 144
           L+GDP +    ELD R++LLI+K
Sbjct: 121 LQGDPRYLPGIELDARIYLLIKK 141

[Close]

BLAST of CSPI04G02730 vs. Swiss-Prot
Match: RPAB3_CAEBR (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.1e-29
Identity = 65/145 (44.83%), Postives = 94/145 (64.83%), Query Frame = 1


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           M  ++FDD+F ++ ++PDGKKFD+V+R    +E F M + +DINS+IYPLK+ +KF + L
Sbjct: 1   MAGIIFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRI--SESSGHGGKAEIDASFGGLL 120
           A TL  DG  D G +          +YEYVM GK++R+   +S   GG+    ASFGGLL
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLL 120

Query: 121 MMLKGDPSHCSKFELDQRLFLLIRK 144
           M LKG+  +   FE+D  L+LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]

BLAST of CSPI04G02730 vs. Swiss-Prot
Match: RPAB3_CAEEL (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.4e-29
Identity = 65/145 (44.83%), Postives = 94/145 (64.83%), Query Frame = 1


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           M  ++FDD+FK++ ++PDGKKFD+V+R    +E F M + +DINS+IYPLK+ +K  + L
Sbjct: 1   MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRI--SESSGHGGKAEIDASFGGLL 120
           A TL  DG  D G +          +YEYVM GK++R+   ++   GGK    ASFGGLL
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLL 120

Query: 121 MMLKGDPSHCSKFELDQRLFLLIRK 144
           M LKG+  +   FE+D  L+LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]

BLAST of CSPI04G02730 vs. TrEMBL
Match: A0A0A0KYV5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G011050 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 8.2e-77
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 1


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CSPI04G02730 vs. TrEMBL
Match: W9RPZ0_9ROSA (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Morus notabilis GN=L484_007786 PE=4 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 4.0e-63
Identity = 117/144 (81.25%), Postives = 131/144 (90.97%), Query Frame = 1


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           M E LF+DIFKI+RLNPDGKKFDKVTRIEAKSEKFDMFMHLD+ +++YP+ EGEKFSMAL
Sbjct: 1   MAEFLFEDIFKIDRLNPDGKKFDKVTRIEAKSEKFDMFMHLDVGTDVYPMNEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           + TLN DGTPDTGY+  GNRK+LAD YEYVMQGKL+RI+E SGH GKAEIDASFGGLLMM
Sbjct: 61  SSTLNEDGTPDTGYYTPGNRKTLADNYEYVMQGKLYRIAEGSGHHGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPS+  K+ELDQRLFLLIRKV
Sbjct: 121 LKGDPSYFKKYELDQRLFLLIRKV 144

[Close]

BLAST of CSPI04G02730 vs. TrEMBL
Match: M5WHF7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011397mg PE=4 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 1.5e-57
Identity = 110/144 (76.39%), Postives = 126/144 (87.50%), Query Frame = 1


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MV+ LF+DIF+I RLNPDGKKFDKVTRIEAKSE  +MFMHLD+NSE+YP++EGEKFSMAL
Sbjct: 69  MVDRLFEDIFQILRLNPDGKKFDKVTRIEAKSETCEMFMHLDVNSEVYPMREGEKFSMAL 128

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
             T+NLDGTPDTGYF QGNRK+LAD YEYVMQGKLF+ISE S    KAE++ASFGGLLMM
Sbjct: 129 TSTINLDGTPDTGYFTQGNRKTLADEYEYVMQGKLFKISEGSKRDPKAEVNASFGGLLMM 188

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKG+ S    FELDQR+FLLIRK+
Sbjct: 189 LKGEASQFKNFELDQRMFLLIRKL 212

[Close]

BLAST of CSPI04G02730 vs. TrEMBL
Match: I3T9W4_LOTJA (Uncharacterized protein OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 2.8e-56
Identity = 107/145 (73.79%), Postives = 130/145 (89.66%), Query Frame = 1


Query: 1   MVELLFDDIFKIERLNPDGKK-FDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMA 60
           MVE+LFDDIF++E+LNPD KK F+KVTRIEA+SE+FDMFMHLDINSE+YPLK G+KF++ 
Sbjct: 1   MVEILFDDIFRVEKLNPDDKKLFEKVTRIEARSERFDMFMHLDINSELYPLKVGQKFALL 60

Query: 61  LAPTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLM 120
           L PTLN DGTPDTGY++ GNR+SLAD +EYVM GKL++ISE SG   KAE++ SFGGLLM
Sbjct: 61  LVPTLNPDGTPDTGYYIPGNRQSLADNFEYVMYGKLYKISEGSGR-EKAELNISFGGLLM 120

Query: 121 MLKGDPSHCSKFELDQRLFLLIRKV 145
           M+KGD SHC+KFELDQRL+LL+RKV
Sbjct: 121 MMKGDHSHCNKFELDQRLYLLMRKV 144

[Close]

BLAST of CSPI04G02730 vs. TrEMBL
Match: A0A058ZTI5_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L01795 PE=4 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 1.4e-55
Identity = 107/149 (71.81%), Postives = 128/149 (85.91%), Query Frame = 1


Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           ++ +D+FK+ERL+PDGKKFDKVTRIEA+SE+FDMFMHLD+N+EIYP+KEG+KF+MALA T
Sbjct: 2   IILEDVFKVERLDPDGKKFDKVTRIEARSERFDMFMHLDVNTEIYPMKEGQKFTMALAST 61

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISE--------SSGHGGKAEIDASFG 123
           LN DGTPDTGY+  GNRKSLAD+YEYVM GKL++ISE        SSG G KAEI  SFG
Sbjct: 62  LNPDGTPDTGYYTPGNRKSLADKYEYVMHGKLYKISEDDPGRASKSSGKGVKAEIYVSFG 121

Query: 124 GLLMMLKGDPSHCSKFELDQRLFLLIRKV 145
           GLLMMLKGDPS+  +F+LDQRLFL IRK+
Sbjct: 122 GLLMMLKGDPSNLHQFDLDQRLFLCIRKL 150

[Close]

BLAST of CSPI04G02730 vs. TAIR10
Match: AT1G54250.1 (AT1G54250.1 RNA polymerase Rpb8)

HSP 1 Score: 211.8 bits (538), Expect = 2.7e-55
Identity = 95/141 (67.38%), Postives = 121/141 (85.82%), Query Frame = 1


Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           +LF+DIF +++L+PDGKKFDKVTR++A S   +MFMHLD+N+E+YPL  G+KF++ALAPT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+LKG
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLKG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of CSPI04G02730 vs. TAIR10
Match: AT3G59600.1 (AT3G59600.1 RNA polymerase Rpb8)

HSP 1 Score: 211.5 bits (537), Expect = 3.6e-55
Identity = 93/141 (65.96%), Postives = 122/141 (86.52%), Query Frame = 1


Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           ++F+DIF +++L+PDGKKFDKVTR+EA+S   +MFMHLD+N+E+YPL  G+KF++A+APT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+L+G
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLQG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of CSPI04G02730 vs. NCBI nr
Match: gi|449468856|ref|XP_004152137.1| (PREDICTED: DNA-directed RNA polymerases II and V subunit 8A [Cucumis sativus])

HSP 1 Score: 294.3 bits (752), Expect = 1.2e-76
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 1


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CSPI04G02730 vs. NCBI nr
Match: gi|659108210|ref|XP_008454076.1| (PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo])

HSP 1 Score: 293.9 bits (751), Expect = 1.5e-76
Identity = 143/144 (99.31%), Postives = 144/144 (100.00%), Query Frame = 1


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+NSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CSPI04G02730 vs. NCBI nr
Match: gi|1009112598|ref|XP_015869217.1| (PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Ziziphus jujuba])

HSP 1 Score: 254.6 bits (649), Expect = 1.0e-64
Identity = 123/144 (85.42%), Postives = 131/144 (90.97%), Query Frame = 1


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           M E LF+DIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDIN+EIYP+KE EKFSMAL
Sbjct: 1   MAEFLFEDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYPMKEREKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           + TLN DGTPDTGY+ Q NRK+LAD YEYVMQGKL+RISE  GHGG AEIDASFGGLLMM
Sbjct: 61  SSTLNEDGTPDTGYYTQANRKTLADNYEYVMQGKLYRISEGPGHGGIAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSH  K+ELDQRLFLLIRKV
Sbjct: 121 LKGDPSHFKKYELDQRLFLLIRKV 144

[Close]

BLAST of CSPI04G02730 vs. NCBI nr
Match: gi|703116084|ref|XP_010101052.1| (DNA-directed RNA polymerases I, II, and III subunit RPABC3 [Morus notabilis])

HSP 1 Score: 248.8 bits (634), Expect = 5.7e-63
Identity = 117/144 (81.25%), Postives = 131/144 (90.97%), Query Frame = 1


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           M E LF+DIFKI+RLNPDGKKFDKVTRIEAKSEKFDMFMHLD+ +++YP+ EGEKFSMAL
Sbjct: 1   MAEFLFEDIFKIDRLNPDGKKFDKVTRIEAKSEKFDMFMHLDVGTDVYPMNEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           + TLN DGTPDTGY+  GNRK+LAD YEYVMQGKL+RI+E SGH GKAEIDASFGGLLMM
Sbjct: 61  SSTLNEDGTPDTGYYTPGNRKTLADNYEYVMQGKLYRIAEGSGHHGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPS+  K+ELDQRLFLLIRKV
Sbjct: 121 LKGDPSYFKKYELDQRLFLLIRKV 144

[Close]

BLAST of CSPI04G02730 vs. NCBI nr
Match: gi|645241925|ref|XP_008227310.1| (PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Prunus mume])

HSP 1 Score: 230.3 bits (586), Expect = 2.1e-57
Identity = 110/144 (76.39%), Postives = 126/144 (87.50%), Query Frame = 1


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MV+ LF+DIF+I RLNPDGKKFDKVTRIEAKSE  +MFMHLD+NSE+YP++EGEKFSMAL
Sbjct: 1   MVDRLFEDIFQILRLNPDGKKFDKVTRIEAKSETCEMFMHLDVNSEVYPMREGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
             T+NLDGTPDTGYF QGNRK+LAD YEYVMQGKLF+ISE S    KAE++ASFGGLLMM
Sbjct: 61  TSTINLDGTPDTGYFTQGNRKTLADEYEYVMQGKLFKISEGSKRDPKAEVNASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKG+ S    FELDQR+FLLIRK+
Sbjct: 121 LKGEASQFKNFELDQRMFLLIRKL 144

The following BLAST results are available for this feature:

BLAST of CSPI04G02730 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RPB8A_ARATH	4.8e-54	67.38	DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana GN=NRPB...	[more]
RPB8B_ARATH	6.3e-54	65.96	DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana GN=...	[more]
RPAB3_DICDI	4.0e-32	46.15	DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium disc...	[more]
RPAB3_CAEBR	1.1e-29	44.83	Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab...	[more]
RPAB3_CAEEL	1.4e-29	44.83	Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab...	[more]

BLAST of CSPI04G02730 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KYV5_CUCSA	8.2e-77	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G011050 PE=4 SV=1	[more]
W9RPZ0_9ROSA	4.0e-63	81.25	DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Morus notabilis GN...	[more]
M5WHF7_PRUPE	1.5e-57	76.39	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011397mg PE=4 SV=1	[more]
I3T9W4_LOTJA	2.8e-56	73.79	Uncharacterized protein OS=Lotus japonicus PE=2 SV=1	[more]
A0A058ZTI5_EUCGR	1.4e-55	71.81	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L01795 PE=4 SV=1	[more]

BLAST of CSPI04G02730 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G54250.1	2.7e-55	67.38	RNA polymerase Rpb8	[more]
AT3G59600.1	3.6e-55	65.96	RNA polymerase Rpb8	[more]

BLAST of CSPI04G02730 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449468856\|ref\|XP_004152137.1\|	1.2e-76	100.00	PREDICTED: DNA-directed RNA polymerases II and V subunit 8A [Cucumis sativus]	[more]
gi\|659108210\|ref\|XP_008454076.1\|	1.5e-76	99.31	PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo]	[more]
gi\|1009112598\|ref\|XP_015869217.1\|	1.0e-64	85.42	PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Ziziphus jujub...	[more]
gi\|703116084\|ref\|XP_010101052.1\|	5.7e-63	81.25	DNA-directed RNA polymerases I, II, and III subunit RPABC3 [Morus notabilis]	[more]
gi\|645241925\|ref\|XP_008227310.1\|	2.1e-57	76.39	PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005570	RNA_pol_Rpb8
IPR012340	NA-bd_OB-fold

Vocabulary: **Biological Process**
Term	Definition
GO:0006351	transcription, DNA-templated

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0006351	transcription, DNA-templated
biological_process	GO:0006383	transcription from RNA polymerase III promoter
biological_process	GO:0006366	transcription from RNA polymerase II promoter
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0009536	plastid
cellular_component	GO:0005736	DNA-directed RNA polymerase I complex
cellular_component	GO:0005665	DNA-directed RNA polymerase II, core complex
cellular_component	GO:0005666	DNA-directed RNA polymerase III complex
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003899	DNA-directed RNA polymerase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G02730.1	CSPI04G02730.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005570	RNA polymerase, Rpb8	PIR	PIRSF000779	RPB8	coord: 1..144 score: 5.7
IPR005570	RNA polymerase, Rpb8	PANTHER	PTHR10917	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3	coord: 1..144 score: 4.9
IPR005570	RNA polymerase, Rpb8	PFAM	PF03870	RNA_pol_Rpb8	coord: 7..143 score: 3.7
IPR005570	RNA polymerase, Rpb8	SMART	SM00658	rpol8neu	coord: 2..143 score: 3.7
IPR012340	Nucleic acid-binding, OB-fold	GENE3D	G3DSA:2.40.50.140		coord: 2..143 score: 6.3
IPR012340	Nucleic acid-binding, OB-fold	unknown	SSF50249	Nucleic acid-binding proteins	coord: 4..143 score: 2.57

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI04G02730	Cla010901	Watermelon (97103) v1	cpiwmB316
CSPI04G02730	Csa4G011050	Cucumber (Chinese Long) v2	cpicuB182
CSPI04G02730	MELO3C017775	Melon (DHL92) v3.5.1	cpimeB310
CSPI04G02730	ClCG07G015110	Watermelon (Charleston Gray)	cpiwcgB337
CSPI04G02730	MELO3C017775.2	Melon (DHL92) v3.6.1	cpimedB301
CSPI04G02730	CsaV3_4G002660	Cucumber (Chinese Long) v3	cpicucB213
CSPI04G02730	Cla97C07G142080	Watermelon (97103) v2	cpiwmbB327
CSPI04G02730	Bhi01G002858	Wax gourd	cpiwgoB381
CSPI04G02730	Cucsa.095410	Cucumber (Gy14) v1	cgycpiB103
CSPI04G02730	CmoCh06G016890	Cucurbita moschata (Rifu)	cmocpiB833
CSPI04G02730	CsGy4G002580	Cucumber (Gy14) v2	cgybcpiB168
CSPI04G02730	Carg15437	Silver-seed gourd	carcpiB0730

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI04G02730	Silver-seed gourd	carcpiB0133
CSPI04G02730	Wax gourd	cpiwgoB376
CSPI04G02730	Cucurbita moschata (Rifu)	cmocpiB250
CSPI04G02730	Cucurbita maxima (Rimu)	cmacpiB263
CSPI04G02730	Cucurbita maxima (Rimu)	cmacpiB850
CSPI04G02730	Cucurbita pepo (Zucchini)	cpecpiB617
CSPI04G02730	Cucurbita pepo (Zucchini)	cpecpiB871
CSPI04G02730	Bottle gourd (USVL1VR-Ls)	cpilsiB311