CsGy3G001160 (gene) Cucumber (Gy14) v2

NameCsGy3G001160
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
DescriptionVacuolar protein sorting-associated protein 25
LocationChr3 : 855729 .. 860114 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCATGAGCTTGCGCTATAAGGTATTAGGTTAAGTCCCACAACTAACACATCCTTCATGTTTAGTTGCTAATGGGTGAGGTTAGAATCCTAAGCAAACGGTCGAGGATAAACATGTATCGAAATTTGAATTTTATGAATTTATGGATAAAATATCTAACATCCACAAATTTTTTTTAAAGTCACAAAATTGCTTCTATAATGAGAGTTTAGATAAAAGCTTTGAAAAACGAATATTTTCTTCCTTCGTTAATGTTCGTTGTCTACCCGGCAAAAAGAAAACCCTCACCTTAGTCTCCTTCTTCCTCACTCTCGCAACGTTCACTCCCCGCGAATACCGAGTGGCACGAACTCCGGTTGACGGCAATCGAACGGCGGCGACAAACACGATTTAGGTATTATTCATTTGTTCCTTTACCCTCTCTTCACTTCCATCTACGATCGCTCAGCGTTGTTTGATCTGAATATCGAAACACGCCATTTGAAGATTGTGATCACACGGGAACTCTGGAACTTCAGCGTTTTCGACATTCAGCTGAATCAAGATCCTGTGAAGGATTTGGAAGTCGATTCTTGAGTTTTGATTTCTCAGTTTATCGTTTTCTCTTACCCTGAAATCAGGCGGTCAATCATTTCGAACACGGCTGGATTTGATTACTGCACTTTTTTAACTTTGAAAATTTGAGTCACTAAATCTCGTTTTCTCTGCGCATCGTTGGTTCTGTGTCTTAATTTTTCATTTTCAATCATTAGATCGATGATTATGATTTTCTTACAGATGTAATAATGAGATTGGGATGACTTTCGAATCATGGAGTGTGTTTTGTTTGTAGTGTTTTGATTAGAGGATTTAAAGTGATCATGGATATTCTAAAACATTTGAGTAGTTTAGGAGTAGTTGTTCAATTTCTTCCAAAAATGTTCTTTACAGCACTTTTTTCAATTTCAAATCCTCCTGAAGTTCTGTGAAATCTTGCCTCGATTAATTTCACAAGCCATTTTACTGTAGGCAATAGCATGCTAGTCATGAAGTTGTTCAGTGTTTCTTCAACAATTTTGCATGTTTAAGTAGTTCCTCAGCCCCTATTTGATAGCCAGTTCAATCTCTTGGTTTGGTTTTTTATCATTGAAAATTAAACTTATAGTCACTTCTTTTACTTTTGGATTTCTTTGGTTTGTTATATTCCTTTTACAAATGTTTTCAAACCCAAGTCAAAGCAAACACTTGGTTTTGTTTTTTGAATTGAACTACCATGTAAAGAAATGGGGGAGAAATTAGGCGCAATTTTCAAGAACCAAATGGATCAAATGGGGTTCTTTTACATGTTAACTATATAGACCCCAAGAGGTGGTGCTCAATCATGGATCTCTTGTCTTCCAACAAAGTCAGACTTGATATTATGGTCTTTTTCAGTCTTGTATCCTGTTACTGTGAAGTTTTCATGCCATTTGATGTTTTTTAGAAATAGCTTCATCAGATGAAATTTATTGTTCGGTTCTCACCACTGATCTGATTCATAGAACACGAAAACTTGATTCTATTTTGACGTGTTCGTTTGCTATCATAATCGGGTACAGGATAAAATTAGAAGAGGAAAGCAACTTGCAGTTCATAACAAGTTTATATAGTTCTTTAATGACAAGGTTTTATTAGTTTGCAAGTCTCTAGTGTCATCGTATCATTTATGAATCTCTGCGTTCTTTTGTCTCTTCATTTTATCTTTAAATTTCTTTCTGAAATTTCTCAGTTCTAGAGATAAGATGCTTCCATAGAACTCAACTCACTTCCAGAGGACACAATAGAGAAGATGCAGAAATTGGGAGATTTCAAGCTACCGCATTTCTTCAATTATCCTCCATATTTCACGTAATGTTCACCATCTGTTTCATTTATTATTCTTTTTACATCATTATTAGCAACTTGTAAGCTGTGTGAGTCAATTTCTGATGATGTTGATATCTTGGTTTTGTCGACAATTTCCTAAGTGAAGAAAACTAACAGGATACAGTATTTACAACCTATAATTTTCCCACCAGCCTCATATATACGAATGTGCTAGTTGTACCTTACCGGAATGTTGATTGTTTCACTTGTCTTCAGTTGCATATATAACCTTTTTATTGCATGTCATCTCAGTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAACTTTGGAAGGAACTTATCCTTGATTACTGCCGAACGCAAAAGATATTTGTCATTGGACTTGAAGAGGACTTTCCTCTATTTTCAAATTCTGTGATTGAAAGTAAATTTCATGGCCTTTTTTGGTCCTAATTGGGTGTTAAAACCTTTTTGTGTTTTAGACTCAGTTGCATAGCTTTGTATTACTGTTTCAGTTGTTTGATGTATTTTATAGCGTGGTTGTGATGCTACAGGATCTCTCAGTCATGAAGCTAGACAAGCTTTTATCTCAGCTTTAGTTTCAGATGGTTAGGGCCTGCTTGTTTAGTTCGATAATTCTATCTGTTTACAGTTCCCATTCTCTTTCATGGGGAAAAAAACTGATACGTGTTCATTTTAATGGTCCTTATAGGTCGTGCAGAATGGCTGGATAAAGGACATAGGAAATGCCTTATTTTATGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTAAGACTAATTACTTTGGTTAAATTAGTCTCAAATTTTTGGTCTATTTGTCCTTGAACTTTAAAAAATGTCCAATAGGTCTTAGTTCAATTCTAAGTTTCATAGATCCTTGAAAATTTTGAAAATGTTAAAAATTATGCGATCCATTAGGTGTAAATTTGGATTTATGCTAATGGAACTCTAAATCAAAGGTTCGGAGTCTAAATTTGTAAATTTGAAAGTTCAAGGTCCAAATAGGCACAAGTTTCAAAAGTTAAATTAAGAGACTGAATTTATTACTTAACCAAATACCATTTTAACTGCCATAGTTATCAGTAAGTTTGAAATGATTTTGAAAGAGTAAAAGTTGCTTAATTCCATTGAAAGAAGTAGGTAAAATTCCACAAGTTTTTTGATAGAAATCAGAAGTGCTTTGAAAATTCGTTATGCTTAAATTGTTTCTTAATAAATAGGTGAAAAATAATGGACTTGAAGATAGTGTTATGACAGTCGAGGAAATACGTTCAGGAGTTGAATCTAGAGGAACAGGTACTTTCTTTGCTTATGTTCTCAAGGTACTAAATGCCCACCTTTCGATGATAATTAATGGAATGAAATAATGAGATTAAACTGCTGATCATTTCACTTTTTAAATATGTTAATATATTTTATTTATTTTATGTTCTCGTTAGTGCCATTTTATATGTTTCTGAATGTGTTCCCAAAGGTGATATTAAAACATTTATTGCACACTGTATAGCTTTTGATAGAATAAGGACATTCTTGAAACAAGGGACAGTTCATAGTTTATCTTTTTTAGCCAATTTGTGTATACAAATTCATTAGCTCAGCCTTAATTGACATGTAATATCTTCATACCTCACATTTGTTGCGTTAAACGTTGTTAGAAACGTATAAGTAGAGTGATGATGTATATTTGTTATTTGCCATGAGCACAGCGTAGCTATAGTTGACATGTACTTCTCTTTGAGCCCCCCAGGCCATACCTTACTTTTTGTTGAACTAAAACTTAATGTAAAAGATTTTACGTAAACATTTCCTCACCAGATCTGTTGTAGAGACAATATATGCTTGCTTTCTGCAAAACTACGTAGCTGTTTTGTGCTTAAACATTACCATATCTTGAAACAGCAATTTTGCTTCATTATAATTTGTAAGAAAAGAGTTATTTGGTTTTGTGTTTTATAAATCAGAGCTTGAAGGGATTGATCGTACGATATTAATGAGAGCATTGAAGCTACTTGAACAAAAGGGGAAGCTTGCAATTTTCAAAGGTTCTTCGACAGATGATGAAGGCATTAAATTCTCTGTATAAACTACCAATGTTTATTATTCACATTGACTGCCAATCTTTCTTTTTTCAACACTTCTAAATGCAACTTTTCATTTAGTTTCCCCCCAATTCCAACTGGGATTGATGGGATTATAGGTTCTTGGTGCAGTTCATTGAATGTAACCAAGTGGACCTCCCAATTGAACTTAAAAGGACCTAAGCTTGGGAGTGAGGATTAAAATATTGATGTCGATATTGATATTAAGGTCTTAAACTTACAGATATATCGACCCAGCGACATATATTTTCATCCTTTTTTCGCGAGTGCTTTGGAAAATATTTGTTGTGCATTGTTGAAAAAAGAACTAAAAACAATCATATAATCCTCCTGTTATTTGGATTGGTTTATGAGATGTGTTTAATTTTTTTAAAAATAAAATTATAGAAATTTTAGGGTAAAA

mRNA sequence

ATCATGAGCTTGCGCTATAAGGTATTAGGTTAAGTCCCACAACTAACACATCCTTCATGTTTAGTTGCTAATGGGTGAGGTTAGAATCCTAAGCAAACGGTCGAGGATAAACATGTATCGAAATTTGAATTTTATGAATTTATGGATAAAATATCTAACATCCACAAATTTTTTTTAAAGTCACAAAATTGCTTCTATAATGAGAGTTTAGATAAAAGCTTTGAAAAACGAATATTTTCTTCCTTCGTTAATGTTCGTTGTCTACCCGGCAAAAAGAAAACCCTCACCTTAGTCTCCTTCTTCCTCACTCTCGCAACGTTCACTCCCCGCGAATACCGAGTGGCACGAACTCCGGTTGACGGCAATCGAACGGCGGCGACAAACACGATTTAGTTCTAGAGATAAGATGCTTCCATAGAACTCAACTCACTTCCAGAGGACACAATAGAGAAGATGCAGAAATTGGGAGATTTCAAGCTACCGCATTTCTTCAATTATCCTCCATATTTCACTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAACTTTGGAAGGAACTTATCCTTGATTACTGCCGAACGCAAAAGATATTTGTCATTGGACTTGAAGAGGACTTTCCTCTATTTTCAAATTCTCGTGGTTGTGATGCTACAGGATCTCTCAGTCATGAAGCTAGACAAGCTTTTATCTCAGCTTTAGTGAAAAATAATGGACTTGAAGATAGTGTTATGACAGTCGAGGAAATACGTTCAGGAGTTGAATCTAGAGGAACAGAGCTTGAAGGGATTGATCGTACGATATTAATGAGAGCATTGAAGCTACTTGAACAAAAGGGGAAGCTTGCAATTTTCAAAGGTTCTTCGACAGATGATGAAGGCATTAAATTCTCTGTATAAACTACCAATGTTTATTATTCACATTGACTGCCAATCTTTCTTTTTTCAACACTTCTAAATGCAACTTTTCATTTAGTTTCCCCCCAATTCCAACTGGGATTGATGGGATTATAGGTTCTTGGTGCAGTTCATTGAATGTAACCAAGTGGACCTCCCAATTGAACTTAAAAGGACCTAAGCTTGGGAGTGAGGATTAAAATATTGATGTCGATATTGATATTAAGGTCTTAAACTTACAGATATATCGACCCAGCGACATATATTTTCATCCTTTTTTCGCGAGTGCTTTGGAAAATATTTGTTGTGCATTGTTGAAAAAAGAACTAAAAACAATCATATAATCCTCCTGTTATTTGGATTGGTTTATGAGATGTGTTTAATTTTTTTAAAAATAAAATTATAGAAATTTTAGGGTAAAA

Coding sequence (CDS)

ATGCAGAAATTGGGAGATTTCAAGCTACCGCATTTCTTCAATTATCCTCCATATTTCACTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAACTTTGGAAGGAACTTATCCTTGATTACTGCCGAACGCAAAAGATATTTGTCATTGGACTTGAAGAGGACTTTCCTCTATTTTCAAATTCTCGTGGTTGTGATGCTACAGGATCTCTCAGTCATGAAGCTAGACAAGCTTTTATCTCAGCTTTAGTGAAAAATAATGGACTTGAAGATAGTGTTATGACAGTCGAGGAAATACGTTCAGGAGTTGAATCTAGAGGAACAGAGCTTGAAGGGATTGATCGTACGATATTAATGAGAGCATTGAAGCTACTTGAACAAAAGGGGAAGCTTGCAATTTTCAAAGGTTCTTCGACAGATGATGAAGGCATTAAATTCTCTGTATAA

Protein sequence

MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLFSNSRGCDATGSLSHEARQAFISALVKNNGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
BLAST of CsGy3G001160 vs. NCBI nr
Match: XP_004146105.1 (PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis sativus] >KGN55713.1 hypothetical protein Csa_3G006740 [Cucumis sativus])

HSP 1 Score: 259.2 bits (661), Expect = 8.4e-66
Identity = 142/182 (78.02%), Postives = 142/182 (78.02%), Query Frame = 0

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSRGCDATGSLSHEARQAFISAL---------------------------------VKN 120
           SNS       SLSHEARQAFISAL                                 VKN
Sbjct: 61  SNS---VIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKN 120

Query: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 150
           NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF
Sbjct: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 179

BLAST of CsGy3G001160 vs. NCBI nr
Match: XP_008448627.1 (PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo])

HSP 1 Score: 257.3 bits (656), Expect = 3.2e-65
Identity = 140/182 (76.92%), Postives = 142/182 (78.02%), Query Frame = 0

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSRGCDATGSLSHEARQAFISAL---------------------------------VKN 120
           SNS       SLSHEARQAF+SAL                                 VKN
Sbjct: 61  SNS---VIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKN 120

Query: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 150
           NGLEDSVMTVEEIRSGVESRGTEL+GIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF
Sbjct: 121 NGLEDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 179

BLAST of CsGy3G001160 vs. NCBI nr
Match: XP_022145259.1 (vacuolar protein sorting-associated protein 25 [Momordica charantia])

HSP 1 Score: 251.1 bits (640), Expect = 2.3e-63
Identity = 134/182 (73.63%), Postives = 142/182 (78.02%), Query Frame = 0

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60

Query: 61  SNSRGCDATGSLSHEARQAFISAL---------------------------------VKN 120
           +N+       SLSHEAR+AF+SAL                                 VKN
Sbjct: 61  TNTA---IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKN 120

Query: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 150
           NGLEDSVMTVEE+RSGVESRGTEL+GIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF
Sbjct: 121 NGLEDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 179

BLAST of CsGy3G001160 vs. NCBI nr
Match: XP_022949655.1 (vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_023005573.1 vacuolar protein sorting-associated protein 25 [Cucurbita maxima])

HSP 1 Score: 249.6 bits (636), Expect = 6.7e-63
Identity = 136/182 (74.73%), Postives = 140/182 (76.92%), Query Frame = 0

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSRGCDATGSLSHEARQAFISAL---------------------------------VKN 120
           SNS       SLSHEAR AF+SAL                                 VKN
Sbjct: 61  SNS---VIERSLSHEARVAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKN 120

Query: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 150
           NGLEDSVMTVEEIRSGVESRGTEL+GIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKF
Sbjct: 121 NGLEDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKF 179

BLAST of CsGy3G001160 vs. NCBI nr
Match: XP_023540532.1 (vacuolar protein sorting-associated protein 25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 248.1 bits (632), Expect = 1.9e-62
Identity = 135/182 (74.18%), Postives = 139/182 (76.37%), Query Frame = 0

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSRGCDATGSLSHEARQAFISAL---------------------------------VKN 120
           SNS       SLSHEAR  F+SAL                                 VKN
Sbjct: 61  SNS---VIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKN 120

Query: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 150
           NGLEDSVMTVEEIRSGVESRGTEL+GIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKF
Sbjct: 121 NGLEDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKF 179

BLAST of CsGy3G001160 vs. TAIR10
Match: AT4G19003.2 (E2F/DP family winged-helix DNA-binding domain)

HSP 1 Score: 230.7 bits (587), Expect = 5.8e-61
Identity = 122/182 (67.03%), Postives = 135/182 (74.18%), Query Frame = 0

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EEDFPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  SNSRGCDATGSLSHEARQAFISAL---------------------------------VKN 120
           SNS       SLSHEAR+ F+SA+                                 V++
Sbjct: 61  SNSA---IDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRD 120

Query: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 150
           NGLEDSVMTVEEIRSG ES GTEL+GIDRTILMRALKLLE KGKLA+FKG+S DDEG+KF
Sbjct: 121 NGLEDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKF 179

BLAST of CsGy3G001160 vs. Swiss-Prot
Match: sp|Q8VZC9|VPS25_ARATH (Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 GN=VPS25 PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 1.0e-59
Identity = 122/182 (67.03%), Postives = 135/182 (74.18%), Query Frame = 0

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EEDFPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  SNSRGCDATGSLSHEARQAFISAL---------------------------------VKN 120
           SNS       SLSHEAR+ F+SA+                                 V++
Sbjct: 61  SNSA---IDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRD 120

Query: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 150
           NGLEDSVMTVEEIRSG ES GTEL+GIDRTILMRALKLLE KGKLA+FKG+S DDEG+KF
Sbjct: 121 NGLEDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKF 179

BLAST of CsGy3G001160 vs. Swiss-Prot
Match: sp|Q6AX45|VPS25_XENLA (Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps25 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 9.9e-18
Identity = 56/176 (31.82%), Postives = 87/176 (49.43%), Query Frame = 0

Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--DFPLFSNSR 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR  K++ + L E  + PLF+N +
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLSAWSSLVLSYCRQNKLYTMNLMEIQESPLFNNKK 62

Query: 67  GCDATGSLSHEARQAFISAL---------------------------------VKNNGLE 126
                  LS E+ Q  +  L                                 V  NG+ 
Sbjct: 63  ---IQRKLSLESVQVVLEELRKKGNLEWIDKNKSRFLIMWRRPDEWGKVIYQWVSKNGMT 122

Query: 127 DSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 148
           +SV T+ E+ SG ++ G E  G+D  +L+R+L+ L+Q+ K  I   +  +  G+KF
Sbjct: 123 NSVFTLYELISGDDTEGEEFHGLDEAMLLRSLEALQQEHKAEII--TLNESRGVKF 173

BLAST of CsGy3G001160 vs. Swiss-Prot
Match: sp|Q55GD9|VPS25_DICDI (Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44689 GN=vps25 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 5.5e-16
Identity = 55/191 (28.80%), Postives = 94/191 (49.21%), Query Frame = 0

Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFP----LFSN 66
           F+ P +++  P+FT+QP+ +TR+KQ Q+W++LIL YCR  KI+ + + E       LF+N
Sbjct: 6   FQFPPYYHKEPFFTIQPILNTRKKQFQMWQDLILQYCRYYKIYELDINESIKSNSVLFNN 65

Query: 67  SRGCDATGSLSHEARQAFISALVKNN---------------------------------- 126
            +       LS EA ++ I  +++N                                   
Sbjct: 66  EK---INRKLSREALKSIIDDIIENGFAEWVDKXXXXXXXXXXXXXXXXXXLIMWRKPDE 125

Query: 127 ------------GLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFK 148
                       GL ++V+TV EI++G +S+  E   ++ TILM++LK+LE++ K   F 
Sbjct: 126 WASLIYKWVADCGLLNTVLTVWEIQNGDDSKKQEFHQLNTTILMKSLKVLEKQSKCQTF- 185

BLAST of CsGy3G001160 vs. Swiss-Prot
Match: sp|Q6NWF4|VPS25_DANRE (Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 PE=2 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 6.0e-15
Identity = 53/176 (30.11%), Postives = 86/176 (48.86%), Query Frame = 0

Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--DFPLFSNSR 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR +K++ + + E  + P+F+N +
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLAAWCSLVLSYCRHRKLYTLDVLEAQESPVFNNKK 62

Query: 67  GCDATGSLSHEARQAFISAL---------------------------------VKNNGLE 126
                  LS EA Q     L                                 V  NG+ 
Sbjct: 63  ---IERKLSVEAIQVVFEELRKKGNLEWLDKNKSRCLIMWRRPEEWGKLIYQWVSKNGMV 122

Query: 127 DSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 148
           +SV T+ E+ +G ++   E  G++  +L+R+L+ L+  GK  I   +  D +G+KF
Sbjct: 123 NSVFTLYELANGDDTEKEEFHGLEDWMLLRSLQALQTDGKAEII--TMDDGKGVKF 173

BLAST of CsGy3G001160 vs. Swiss-Prot
Match: sp|Q9CQ80|VPS25_MOUSE (Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.3e-12
Identity = 52/176 (29.55%), Postives = 80/176 (45.45%), Query Frame = 0

Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCR--TQKIFVIGLEEDFPLFSNSR 66
           F+ P  + +PP+FTLQP  DTR+KQ+  W  L+L +CR   Q    +   ++ PLF+N +
Sbjct: 5   FEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVK 64

Query: 67  GCDATGSLSHEARQAFISALVKNNGLE--------------------------------- 126
                  L  E+ Q  +  L K   LE                                 
Sbjct: 65  ---LQRKLPVESIQIVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSRSGQN 124

Query: 127 DSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 148
           +SV T+ E+ SG ++   E  G+D   L+RAL+ L+Q+ K  I   + +D  G+KF
Sbjct: 125 NSVFTLYELTSGEDTEDEEFHGLDEATLLRALQALQQEHKAEII--TVSDGRGVKF 175

BLAST of CsGy3G001160 vs. TrEMBL
Match: tr|A0A0A0L1R7|A0A0A0L1R7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G006740 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 5.6e-66
Identity = 142/182 (78.02%), Postives = 142/182 (78.02%), Query Frame = 0

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSRGCDATGSLSHEARQAFISAL---------------------------------VKN 120
           SNS       SLSHEARQAFISAL                                 VKN
Sbjct: 61  SNS---VIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKN 120

Query: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 150
           NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF
Sbjct: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 179

BLAST of CsGy3G001160 vs. TrEMBL
Match: tr|A0A1S3BKR8|A0A1S3BKR8_CUCME (vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490745 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.1e-65
Identity = 140/182 (76.92%), Postives = 142/182 (78.02%), Query Frame = 0

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSRGCDATGSLSHEARQAFISAL---------------------------------VKN 120
           SNS       SLSHEARQAF+SAL                                 VKN
Sbjct: 61  SNS---VIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKN 120

Query: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 150
           NGLEDSVMTVEEIRSGVESRGTEL+GIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF
Sbjct: 121 NGLEDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 179

BLAST of CsGy3G001160 vs. TrEMBL
Match: tr|A0A2I4H7U9|A0A2I4H7U9_9ROSI (vacuolar protein sorting-associated protein 25 OS=Juglans regia OX=51240 GN=LOC109014272 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 7.1e-61
Identity = 128/182 (70.33%), Postives = 138/182 (75.82%), Query Frame = 0

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTR+KQ+QLWKELILDYCRTQKIFV+GLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTRDKQVQLWKELILDYCRTQKIFVVGLEEDFPLF 60

Query: 61  SNSRGCDATGSLSHEARQAFISAL---------------------------------VKN 120
           SN        SLSHEAR+AF+ AL                                 VK+
Sbjct: 61  SNP---VVERSLSHEAREAFLLALVSEGRAEWLDKGHRKCLILWHRIQEWADIILSFVKD 120

Query: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 150
           NGLEDSVMTVEEIRSG ESRGTEL GIDRTILMRAL+LLEQKGKLAIFKG+STDDEG+KF
Sbjct: 121 NGLEDSVMTVEEIRSGAESRGTELHGIDRTILMRALRLLEQKGKLAIFKGTSTDDEGVKF 179

BLAST of CsGy3G001160 vs. TrEMBL
Match: tr|A0A1R3JF65|A0A1R3JF65_9ROSI (ESCRT-II complex, vps25 subunit OS=Corchorus olitorius OX=93759 GN=COLO4_16920 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 7.1e-61
Identity = 128/182 (70.33%), Postives = 139/182 (76.37%), Query Frame = 0

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVR+TREKQ+QLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRETREKQVQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSRGCDATGSLSHEARQAFISAL---------------------------------VKN 120
           SNS       +L+HEAR+AF+SAL                                 VK+
Sbjct: 61  SNS---VIERTLTHEAREAFLSALVSEGRAEWLDKGRRKCLILWHRIQDWADIILNFVKD 120

Query: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 150
           NG EDSVMTVEEIRSG+ESRGTEL GIDRTILMRALKLLEQKGKLAIFKG+S DDEG+KF
Sbjct: 121 NGFEDSVMTVEEIRSGIESRGTELHGIDRTILMRALKLLEQKGKLAIFKGTSADDEGVKF 179

BLAST of CsGy3G001160 vs. TrEMBL
Match: tr|A0A0D2QMU3|A0A0D2QMU3_GOSRA (Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_003G186000 PE=4 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 9.2e-61
Identity = 128/182 (70.33%), Postives = 139/182 (76.37%), Query Frame = 0

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVR+TREKQIQLWKELILDYCRTQKIF++ LEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRETREKQIQLWKELILDYCRTQKIFIVRLEEEFPLF 60

Query: 61  SNSRGCDATGSLSHEARQAFISAL---------------------------------VKN 120
           SNS       +LSHEAR+AF+SAL                                 VK+
Sbjct: 61  SNS---VIERTLSHEAREAFLSALVAEGRAEWLDKGRRNCLILWHRIQEWADIIVNFVKD 120

Query: 121 NGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 150
           NG EDSVMTVEEIRSG+ESRGTEL GIDRTILMRALKLLEQKGKLAIFKG+STDDEG+KF
Sbjct: 121 NGFEDSVMTVEEIRSGIESRGTELHGIDRTILMRALKLLEQKGKLAIFKGTSTDDEGVKF 179

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004146105.18.4e-6678.02PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis sa... [more]
XP_008448627.13.2e-6576.92PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis me... [more]
XP_022145259.12.3e-6373.63vacuolar protein sorting-associated protein 25 [Momordica charantia][more]
XP_022949655.16.7e-6374.73vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_02300557... [more]
XP_023540532.11.9e-6274.18vacuolar protein sorting-associated protein 25 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G19003.25.8e-6167.03E2F/DP family winged-helix DNA-binding domain[more]
Match NameE-valueIdentityDescription
sp|Q8VZC9|VPS25_ARATH1.0e-5967.03Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 G... [more]
sp|Q6AX45|VPS25_XENLA9.9e-1831.82Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps2... [more]
sp|Q55GD9|VPS25_DICDI5.5e-1628.80Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44... [more]
sp|Q6NWF4|VPS25_DANRE6.0e-1530.11Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 P... [more]
sp|Q9CQ80|VPS25_MOUSE1.3e-1229.55Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0L1R7|A0A0A0L1R7_CUCSA5.6e-6678.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G006740 PE=4 SV=1[more]
tr|A0A1S3BKR8|A0A1S3BKR8_CUCME2.1e-6576.92vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo OX=365... [more]
tr|A0A2I4H7U9|A0A2I4H7U9_9ROSI7.1e-6170.33vacuolar protein sorting-associated protein 25 OS=Juglans regia OX=51240 GN=LOC1... [more]
tr|A0A1R3JF65|A0A1R3JF65_9ROSI7.1e-6170.33ESCRT-II complex, vps25 subunit OS=Corchorus olitorius OX=93759 GN=COLO4_16920 P... [more]
tr|A0A0D2QMU3|A0A0D2QMU3_GOSRA9.2e-6170.33Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_003G186000 PE=4 ... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0071985multivesicular body sorting pathway
Vocabulary: Cellular Component
TermDefinition
GO:0000814ESCRT II complex
Vocabulary: INTERPRO
TermDefinition
IPR036390WH_DNA-bd_sf
IPR008570ESCRT-II_cplx_Vps25-sub
IPR014041ESCRT-II_cplx_Vps25-sub_N
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071985 multivesicular body sorting pathway
cellular_component GO:0000814 ESCRT II complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G001160.1CsGy3G001160.1mRNA


Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014041ESCRT-II complex, Vps25 subunit, N-terminal winged helixGENE3DG3DSA:1.10.10.570coord: 4..100
e-value: 7.2E-20
score: 73.3
IPR008570ESCRT-II complex, Vps25 subunitPFAMPF05871ESCRT-IIcoord: 13..86
e-value: 6.4E-16
score: 58.7
IPR008570ESCRT-II complex, Vps25 subunitPANTHERPTHR13149VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25coord: 5..87
coord: 78..147
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILYSSF46785"Winged helix" DNA-binding domaincoord: 81..147
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILYSSF46785"Winged helix" DNA-binding domaincoord: 7..91

The following gene(s) are paralogous to this gene:

None