Cp4.1LG08g08850 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG08g08850
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionVacuolar protein sorting-associated protein 25
LocationCp4.1LG08 : 6976742 .. 6981850 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGGCCGAGGAAACTCATCATCTTGGGCCCGAAGTGGGCTCGAGTTTTCTTCTGCAGCGATGATCCGAACTGTTTCGGTCTTGCTTCTTGGTTAGCCGATTGACGAAGCCGAAATTCGATACTTGGCGAGCACAAAGCACGATTTTATTGGCAACCAATCCAAAGATCTTCTTGTTTTCGCGATTGATTACAGGTACAATTTAAGTTTCTTTTTCTGTCTCTAAATTTCCTTTTTGCGTCCACCGATTGATCTAAATATCGTAACACACTGATGGAAAATTATGATTACACTGCGCTTGAGGAGAGTTTTGAAAGATTTTTGTATATCTGCATTGGATACGCGAGATTCTGTGGAAAAATTCAATTCCAGTTTGGAGTTTTTGATTCTCAGTTTTTTTGGCTTCTTTTACTCTGAATCAAGCATGAATTATTCCGAAAATGGCTGGATATGTTTAGTTCTATTATTCCAAACTTTTCAAGGACGATAATTTTAGCTACCAAATCGTGTTTTGCTTGCGCGTCGAGCGGCAATCTGTTATCAAGCATTAGATCGTAGATAATGATCATCTTGCAAAGTTTGGGATTACTTTAGGATCATTAATGTGATTTTAACCCGTTAAAATACTTGTAAATACTTAAAATCGTTCCAGACTCACTTGAAGTCATCATCTTTGTTTGGTTTCGCTTCATTCTATTTCATATGCCATTTTCCTTTAGGCAATAGTATGCTAGTTATGAAGTTGTTCGGTATTTCTTCAACAAATTTGCTTGTTCAAGTCGATGCACAAAAGGTGCCTTATCACGAATCTCTTATCATCCAAGATATTTCCAGTACTATGGGTCCTTTCAGTATGGTACTTTATGCTTCTGAAGTTATCATGCCATATGCTGTCTTGTAGCTATAGCTTCACCATACCAATTTTACCTTACAGTTCGATCTGATTCTTGAAAACACGGAAACTTGATTCTACTTTGACATGCTTATTTCCAATCATAATCAACAACAGGAAAAAAATTTAGGAAACTTTATACTAGCTATGGAATGCAAGGCTCGTCTTAGTTTGCAAGTCTCTAGTCTCATTGAACCATTTACACCACTAAAATAGTTATGCATCAATGGAAAGTGGTTAGGCCTGTCATGTTTATGAATCTCTGCGTTAATTTATCTCTTTATGTTATTGATATTGTGCTAAGTTTCTTCCCACCCATCTGAAATTTCTCAGTTATAGAGAGAAATGCATCCATAGGACTCATTTTCAGAGAGCGCAATAGAGAAAATGCAGAAATTGGGAGATTTCAAGCTACCACATTTCTTCAATTACCCTCCATATTTCACGTAATTTCCTTTTCCTGTTTCAGTTACCATGGTTTTTTACATGTTTATTTGCTTAACCAAGTAACTTGGAAGTTGCGAGTCAATTTCTGAAGATGTTGATCTCTTGGTTTTGATTGCTTTTCCCTGAGTGAATAAAACCAACACTTCATTGCTCAGCCAGTAGTTATGCAACAATAACAGGGTTCTGGATCACTTATGCTTCTCCGTTTGCCTCATAAGAATGAATCTGATAATTTTTTTTTCCATAAAAAATGAATTTGGTAATTCTACCTTGCTGCAATTCTGAAATTGTTATTGATAACTTCGACTGTTTCACTTTATATCAAGCTTGTCATGTCTTAAGTTTCACTTATAATCTTGTATTGCATGTCTTATCAGTTTGCAACCGGTGAAGGACACCCGTGAGAAGCAAATTCAACTTTGGAAGGAACTTATTCTCGACTACTGCCGAACACAAAAGATATTTGTCATTGGACTCGAGGAGGAATTTCCTCTATTTTCAAATTCTGTCATTGAGAGTAAATTCCTGAACTTGTTTGGTCCTCATTGACCGTTAAGACTTTTGGTACATGCATTAATATCAACGCTAATGAAGTCATTCACAGTTGCATCATGAACAGTGTGAAAAACATTAAGATATTGGCTTCATTATATGAAACCTTTTCATTCTATGGAGCATAGTACATTGGACTTGGTGTTTAGAACTCTCAGTCATGGATCTATGTTCTACTATTCAGTTGTTTGATGTATTTTACAGTGTGGTTGTGATGCTACAGGATCTCTCAGTCATGAAGCTAGAGTAGTTTTTCTCTCTGCCTTAGTTTCAGATGGTTAGTCCGTTCTAGTTTTGTTCTATCATTATATTTGTTTACAGTTTCCATTTATTCCCAAAACTGATAAATTGTTACTTTAATTGTCCAATAGGTCGTGCAGAATGGCTGGATAAGGGACATAGGAAATGTCTTATTTTGTGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTAAGACTAAATACTTTGTTAACTCTCATAGTTATGTTAAAGTTGATTGGTTTAGGGTGGAGTTTGGAATGATTTTTGAAAGGGTAATAAATGCTATTATCCTATTAAAGCTGTTTTCAAAGTATTCTAGTCTTCTGGTTGCATTTGGATTTGATTAGGAGGAAATTTTAACATTCTTTAATGTTGACTGATTATAAATTAAGCTAAAGAAAATACCTTTTCAGTTGTGTTGGTTTGTAAAATTTTGTTAAGCGCATCTTTTGACTTCTCAAAGAGTTTGATGCCTCTACTGACGAACTCTTGAAAATCCTTTTTACCCATATTTTTCCACATTTCCACACTTATTTACTTTATATTACAACTGATTTTTTTTAATAAAGAAATTAAAACAATTATTTCAAAAACTACAACAATTATTTTAAAGAAGGACAACATTCTCAAATTGAGCCTGTTTGGAAAGATTGTTCGAGTAATTATGAACATTTTTGTAACACTTAAAATCCAAATCATTCCAAACAAACTCTATTCCAGGGGTGCTTTGAAAAATTCTTATGCAATTGCCTTATTAAATAGGTGAAAAACAATGGACTTGAAGATAGTGTTATGACAGTTGAGGAAATACGTTCAGGAGTTGAGTCCAGAGGAACAGGTACTTTCCTTGCTTCTACTGAGGCGATTTAGATCTTCCAATGATTTCTAAATCTATTTTTAATTTGCTACATTGATATTCGTATTAAAGCAACTCTAGTTGGCTATCCCTCAGGTGTTATTTTGTTTAATTGGAGCCTGATTTTATTTTAGTTTGGCTTCTTTTGTTTGGGGTTAATTTCTTCTTGTACCTTCTTGCATTTTTTAATACTTTTTCTCAATGAAAGTTCACTTCCCTCAAAAAGTAAAAAGAAAAAAAGGGGGGAAAAAGATGATGGAAAACATCTTGGTGCTTAATAAATCCAAGAAACTTGGCTCAAGGGGAAATGTAACACTGGCAAAACTGATATGAACAATTAGTTGGGGCGTAGGTCCTANAATTTTACTTCCCCACCTAGCTACTCTAGGTTGGGGCGTAGGTCCTGGTGGGGAAGTTATAGACACCTTTCCGTACTTTGTGTCTGGAGTACTTCACTTAATTTCCTCCGCAGTATTGGGTTTTGGCGGTATTTATCATGCACTTCTGGGACCCGAGACCCTTGAAGAATCTTTTCCATTCTTCGGTTATGTGTGGAAAGATAGAAATAAAATGACTACCATTTTGGGTATTCACTTAATTTTGTTAGGTATAGGTGCTTTTCTTCTAGTATTCAAAGCTCTTTATTTTGGGGGCGTATATGATACCTGGGCTCCGGGGGGGGGAGATGTAAGAAAAATTACCAACTTGACCCTTAGCCCAAGTGTTATATTTGGTTATTTACTAAAATCTCCTTTTGGGGGAGAAGGATGGATTGTTAGTGTGGACGATTTGGAAGATATAGTTGGGGGGCATGTATGGTTAGGTTCCATTTGTATACTTGGTGGAATTTGGCATATCTTAACTAAACCGTTTGCATGGGCTCGCCGCGCACTTGTATGGTCTGGTTTAGTTTAAGTCTCATTTTATTTGTAAACTTTTAAACCTGTCAACTTAAGATATACCGGAGCCATTAATATAGATAACTTTCTCCTTACATTCATATTCAAAAGAAAAACAAAAAGGTATTGAAGATAGAAGTTTAATAACTCAGATCACTTACTTTTTATGGGCAAACAAATTAGTTACGTAGATGAGAGTCAGAAAATCATTATTTGAATAAGAAGGAATGTCAGGGAATAGAATCAAAGGTTCTGGAATTGTACTAGTTTTTGCTTTCATTTCCTCTCTAGTGTAAAAGAATATCTGATTCTGGTTCTGGAAGCCACACACAAGAAAGAAACTTTTCTTATATTTGTAAATGTCTACTTTGGTTTCTTTGGTCCTTGCCGATCGATAGATTATATTTAACAAATTTGATGCCAAATGAAGTGGATGAGACTCGAAGCATGAACTTAACTCATGCTGAGTCATGTAAGAGTTTGGTTAAATTCTCTAAAAAGGTTCATAGTAAAGACAAATAATCTTTTACACAAAATTCATGAATCAAATAGGATTATGCTTTTGATACTTGCATTACTAAATGTTTACATCCTTTAATCACATTGAAGCCTTATTTGTGCAAACCAATACGTTTATTTTTCAGAGCTTCAAGGGATTGACCGTACGATTTTAATGAGAGCATTGAAGCAACTTGAACAGAAGGGAAAGCTTGCAATTTTCAAGGGTTCTTCAACAGACGATGAAGGCATTAAATTCTCTGTATGACTCCAAAATCGAACGGCCTCAATGTGAATTTTCCAGTTCAATCTTCTTCTAAATGATACTCATCTGTAGTTTCCCTGCCTATTCAAACTGGGATTGTGAAGAGTTCTTCTTGCCAAAGTTTTGGTGCAGCATTTCAGTGTAGTAATGTAACCAATAAGTAAAGAGCCTTCAAGTAGAACTCAAGAAACTATGCTTGGAAGTGCTTTGGAAGGATATGGTATTTTGTGTAGCCTTTTGAAGTTCTTTTAAATGTATTTTTATTATCAGTTGGAATGATTTGAATAGTAACAAGATTAGAAGTGGGAATTGGGGAACTGTAATTCTGCTAAGTAAATGGTTATATCAATTATTTTATCCTAAAAAATTGATTTATTTGGATTTTGTTTTGTAGTTATTTGATTTTTTATTTTTAAAAAATATTGCTTGT

mRNA sequence

AGAGGCCGAGGAAACTCATCATCTTGGGCCCGAAGTGGGCTCGAGTTTTCTTCTGCAGCGATGATCCGAACTGTTTCGGTCTTGCTTCTTGGTTAGCCGATTGACGAAGCCGAAATTCGATACTTGGCGAGCACAAAGCACGATTTTATTGGCAACCAATCCAAAGATCTTCTTGTTTTCGCGATTGATTACAGTTATAGAGAGAAATGCATCCATAGGACTCATTTTCAGAGAGCGCAATAGAGAAAATGCAGAAATTGGGAGATTTCAAGCTACCACATTTCTTCAATTACCCTCCATATTTCACTTTGCAACCGGTGAAGGACACCCGTGAGAAGCAAATTCAACTTTGGAAGGAACTTATTCTCGACTACTGCCGAACACAAAAGATATTTGTCATTGGACTCGAGGAGGAATTTCCTCTATTTTCAAATTCTGTCATTGAGAGATCTCTCAGTCATGAAGCTAGAGTAGTTTTTCTCTCTGCCTTAGTTTCAGATGGTCGTGCAGAATGGCTGGATAAGGGACATAGGAAATGTCTTATTTTGTGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTGAAAAACAATGGACTTGAAGATAGTGTTATGACAGTTGAGGAAATACGTTCAGGAGTTGAGTCCAGAGGAACAGAGCTTCAAGGGATTGACCGTACGATTTTAATGAGAGCATTGAAGCAACTTGAACAGAAGGGAAAGCTTGCAATTTTCAAGGGTTCTTCAACAGACGATGAAGGCATTAAATTCTCTGTATGACTCCAAAATCGAACGGCCTCAATGTGAATTTTCCAGTTCAATCTTCTTCTAAATGATACTCATCTGTAGTTTCCCTGCCTATTCAAACTGGGATTGTGAAGAGTTCTTCTTGCCAAAGTTTTGGTGCAGCATTTCAGTGTAGTAATGTAACCAATAAGTAAAGAGCCTTCAAGTAGAACTCAAGAAACTATGCTTGGAAGTGCTTTGGAAGGATATGGTATTTTGTGTAGCCTTTTGAAGTTCTTTTAAATGTATTTTTATTATCAGTTGGAATGATTTGAATAGTAACAAGATTAGAAGTGGGAATTGGGGAACTGTAATTCTGCTAAGTAAATGGTTATATCAATTATTTTATCCTAAAAAATTGATTTATTTGGATTTTGTTTTGTAGTTATTTGATTTTTTATTTTTAAAAAATATTGCTTGT

Coding sequence (CDS)

ATGCAGAAATTGGGAGATTTCAAGCTACCACATTTCTTCAATTACCCTCCATATTTCACTTTGCAACCGGTGAAGGACACCCGTGAGAAGCAAATTCAACTTTGGAAGGAACTTATTCTCGACTACTGCCGAACACAAAAGATATTTGTCATTGGACTCGAGGAGGAATTTCCTCTATTTTCAAATTCTGTCATTGAGAGATCTCTCAGTCATGAAGCTAGAGTAGTTTTTCTCTCTGCCTTAGTTTCAGATGGTCGTGCAGAATGGCTGGATAAGGGACATAGGAAATGTCTTATTTTGTGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTGAAAAACAATGGACTTGAAGATAGTGTTATGACAGTTGAGGAAATACGTTCAGGAGTTGAGTCCAGAGGAACAGAGCTTCAAGGGATTGACCGTACGATTTTAATGAGAGCATTGAAGCAACTTGAACAGAAGGGAAAGCTTGCAATTTTCAAGGGTTCTTCAACAGACGATGAAGGCATTAAATTCTCTGTATGA

Protein sequence

MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLFSNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV
BLAST of Cp4.1LG08g08850 vs. Swiss-Prot
Match: VPS25_ARATH (Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana GN=VPS25 PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 2.4e-87
Identity = 152/179 (84.92%), Postives = 167/179 (93.30%), Query Frame = 1

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKL DFKLP FFNYPPYFTLQPV+DTREKQIQLWKELILDYC++QKIF+IG+EE+FPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNS I+RSLSHEAR  FLSA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG ES GTELQGIDRTILMRALK LE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

BLAST of Cp4.1LG08g08850 vs. Swiss-Prot
Match: VPS25_XENLA (Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis GN=vps25 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.8e-34
Identity = 71/173 (41.04%), Postives = 106/173 (61.27%), Query Frame = 1

Query: 7   FKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEE--EFPLFSNSV 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR  K++ + L E  E PLF+N  
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLSAWSSLVLSYCRQNKLYTMNLMEIQESPLFNNKK 62

Query: 67  IERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           I+R LS E+  V L  L   G  EW+DK   + LI+W R  +W  +I  +V  NG+ +SV
Sbjct: 63  IQRKLSLESVQVVLEELRKKGNLEWIDKNKSRFLIMWRRPDEWGKVIYQWVSKNGMTNSV 122

Query: 127 MTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ SG ++ G E  G+D  +L+R+L+ L+Q+ K  I   +  +  G+KF
Sbjct: 123 FTLYELISGDDTEGEEFHGLDEAMLLRSLEALQQEHKAEII--TLNESRGVKF 173

BLAST of Cp4.1LG08g08850 vs. Swiss-Prot
Match: VPS25_DANRE (Vacuolar protein-sorting-associated protein 25 OS=Danio rerio GN=vps25 PE=2 SV=2)

HSP 1 Score: 143.7 bits (361), Expect = 2.0e-33
Identity = 70/173 (40.46%), Postives = 107/173 (61.85%), Query Frame = 1

Query: 7   FKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEE--EFPLFSNSV 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR +K++ + + E  E P+F+N  
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLAAWCSLVLSYCRHRKLYTLDVLEAQESPVFNNKK 62

Query: 67  IERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           IER LS EA  V    L   G  EWLDK   +CLI+W R ++W  +I  +V  NG+ +SV
Sbjct: 63  IERKLSVEAIQVVFEELRKKGNLEWLDKNKSRCLIMWRRPEEWGKLIYQWVSKNGMVNSV 122

Query: 127 MTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ +G ++   E  G++  +L+R+L+ L+  GK  I   +  D +G+KF
Sbjct: 123 FTLYELANGDDTEKEEFHGLEDWMLLRSLQALQTDGKAEII--TMDDGKGVKF 173

BLAST of Cp4.1LG08g08850 vs. Swiss-Prot
Match: VPS25_DICDI (Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum GN=vps25 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.0e-30
Identity = 68/188 (36.17%), Postives = 113/188 (60.11%), Query Frame = 1

Query: 7   FKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFP----LFSN 66
           F+ P +++  P+FT+QP+ +TR+KQ Q+W++LIL YCR  KI+ + + E       LF+N
Sbjct: 6   FQFPPYYHKEPFFTIQPILNTRKKQFQMWQDLILQYCRYYKIYELDINESIKSNSVLFNN 65

Query: 67  SVIERSLSHEARVVFLSALVSDGRAEWLDK-------------GHRKCLILWHRIQDWAD 126
             I R LS EA    +  ++ +G AEW+DK              + + LI+W +  +WA 
Sbjct: 66  EKINRKLSREALKSIIDDIIENGFAEWVDKEKEKEKEKDKDNNNNNRVLIMWRKPDEWAS 125

Query: 127 IILHFVKNNGLEDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSS 178
           +I  +V + GL ++V+TV EI++G +S+  E   ++ TILM++LK LE++ K   F  S 
Sbjct: 126 LIYKWVADCGLLNTVLTVWEIQNGDDSKKQEFHQLNTTILMKSLKVLEKQSKCQTF--SQ 185

BLAST of Cp4.1LG08g08850 vs. Swiss-Prot
Match: VPS25_MOUSE (Vacuolar protein-sorting-associated protein 25 OS=Mus musculus GN=Vps25 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.1e-29
Identity = 66/173 (38.15%), Postives = 100/173 (57.80%), Query Frame = 1

Query: 7   FKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEE--EFPLFSNSV 66
           F+ P  + +PP+FTLQP  DTR+KQ+  W  L+L +CR  K   + + E  E PLF+N  
Sbjct: 5   FEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVK 64

Query: 67  IERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           ++R L  E+  + L  L   G  EWLDK     LI+W R ++W  +I  +V  +G  +SV
Sbjct: 65  LQRKLPVESIQIVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSV 124

Query: 127 MTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ SG ++   E  G+D   L+RAL+ L+Q+ K  I   + +D  G+KF
Sbjct: 125 FTLYELTSGEDTEDEEFHGLDEATLLRALQALQQEHKAEII--TVSDGRGVKF 175

BLAST of Cp4.1LG08g08850 vs. TrEMBL
Match: A0A0A0L1R7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G006740 PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 6.3e-95
Identity = 172/179 (96.09%), Postives = 176/179 (98.32%), Query Frame = 1

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEAR  F+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTEL+GIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

BLAST of Cp4.1LG08g08850 vs. TrEMBL
Match: B9MU83_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s01810g PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 1.2e-88
Identity = 159/179 (88.83%), Postives = 171/179 (95.53%), Query Frame = 1

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLP FFNYPPYFTLQPV+DTREKQ+QLWKELILDYCRTQKIFVIGLEEEFPLF
Sbjct: 1   MQKLGDFKLPQFFNYPPYFTLQPVRDTREKQVQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SN +IERSLS+EAR  FLSALVS+GRAEWLD+GHRKCLILWHRIQDWADI+LHFV++NG 
Sbjct: 61  SNHLIERSLSNEAREAFLSALVSEGRAEWLDRGHRKCLILWHRIQDWADILLHFVRDNGF 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIR+GVESRGTEL GIDRTILMRALK LE KGKLAIFKG+STDDEG+KFSV
Sbjct: 121 EDSVMTVEEIRTGVESRGTELHGIDRTILMRALKLLEHKGKLAIFKGTSTDDEGVKFSV 179

BLAST of Cp4.1LG08g08850 vs. TrEMBL
Match: A0A067JBX1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21748 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 2.0e-88
Identity = 159/179 (88.83%), Postives = 169/179 (94.41%), Query Frame = 1

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLP FFNYPPYFTLQPV+DTREKQ+QLWKELILDYCRTQ+IF I LEEEFPLF
Sbjct: 1   MQKLGDFKLPQFFNYPPYFTLQPVRDTREKQVQLWKELILDYCRTQQIFAIELEEEFPLF 60

Query: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SN VIERSLSHEAR  FLSALVS+GRAEWLDKGHRKCLILWHRIQDWA+IILHFVK+NGL
Sbjct: 61  SNPVIERSLSHEARKAFLSALVSEGRAEWLDKGHRKCLILWHRIQDWANIILHFVKDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           ED VMT+EEIRSG+ESRGTEL GIDRTILMRALK LE KGKLAIFKG+STDDEG+KFSV
Sbjct: 121 EDGVMTIEEIRSGIESRGTELHGIDRTILMRALKLLESKGKLAIFKGTSTDDEGVKFSV 179

BLAST of Cp4.1LG08g08850 vs. TrEMBL
Match: A0A061G5Y1_THECC (E2F/DP family winged-helix DNA-binding domain isoform 1 OS=Theobroma cacao GN=TCM_016610 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 2.0e-88
Identity = 158/179 (88.27%), Postives = 170/179 (94.97%), Query Frame = 1

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV++TREKQ+QLWK+LILDYCRTQKIF+IGLEEEFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRETREKQVQLWKDLILDYCRTQKIFIIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SN VIER+LSHEAR  F SALVS+GRAEWLDKGHRKCLILWHRIQDWA IIL+FVK+NG 
Sbjct: 61  SNPVIERTLSHEAREAFFSALVSEGRAEWLDKGHRKCLILWHRIQDWAGIILNFVKDNGF 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG+ESRGTEL GIDRTILMRALK LEQKGKLAIFKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGIESRGTELHGIDRTILMRALKILEQKGKLAIFKGTSADDEGVKFSV 179

BLAST of Cp4.1LG08g08850 vs. TrEMBL
Match: A0A0D2QMU3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_003G186000 PE=4 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 9.8e-88
Identity = 157/179 (87.71%), Postives = 171/179 (95.53%), Query Frame = 1

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV++TREKQIQLWKELILDYCRTQKIF++ LEEEFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRETREKQIQLWKELILDYCRTQKIFIVRLEEEFPLF 60

Query: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIER+LSHEAR  FLSALV++GRAEWLDKG R CLILWHRIQ+WADII++FVK+NG 
Sbjct: 61  SNSVIERTLSHEAREAFLSALVAEGRAEWLDKGRRNCLILWHRIQEWADIIVNFVKDNGF 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG+ESRGTEL GIDRTILMRALK LEQKGKLAIFKG+STDDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGIESRGTELHGIDRTILMRALKLLEQKGKLAIFKGTSTDDEGVKFSV 179

BLAST of Cp4.1LG08g08850 vs. TAIR10
Match: AT4G19003.2 (AT4G19003.2 E2F/DP family winged-helix DNA-binding domain)

HSP 1 Score: 322.8 bits (826), Expect = 1.4e-88
Identity = 152/179 (84.92%), Postives = 167/179 (93.30%), Query Frame = 1

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKL DFKLP FFNYPPYFTLQPV+DTREKQIQLWKELILDYC++QKIF+IG+EE+FPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNS I+RSLSHEAR  FLSA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG ES GTELQGIDRTILMRALK LE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

BLAST of Cp4.1LG08g08850 vs. NCBI nr
Match: gi|659095525|ref|XP_008448627.1| (PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo])

HSP 1 Score: 356.7 bits (914), Expect = 2.4e-95
Identity = 174/179 (97.21%), Postives = 176/179 (98.32%), Query Frame = 1

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEAR  FLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTELQGIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

BLAST of Cp4.1LG08g08850 vs. NCBI nr
Match: gi|449456737|ref|XP_004146105.1| (PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis sativus])

HSP 1 Score: 354.8 bits (909), Expect = 9.1e-95
Identity = 172/179 (96.09%), Postives = 176/179 (98.32%), Query Frame = 1

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEAR  F+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTEL+GIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

BLAST of Cp4.1LG08g08850 vs. NCBI nr
Match: gi|1009158488|ref|XP_015897317.1| (PREDICTED: vacuolar protein sorting-associated protein 25-like [Ziziphus jujuba])

HSP 1 Score: 339.7 bits (870), Expect = 3.0e-90
Identity = 163/179 (91.06%), Postives = 172/179 (96.09%), Query Frame = 1

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQ+QLWKELILDYCRTQKIFVIGLEEEFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQVQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SN VIER+LSHEAR  FLSALVS+GRAEWLDKGHRKCLILWHRIQ+WADIIL+FVK+NGL
Sbjct: 61  SNPVIERTLSHEAREAFLSALVSEGRAEWLDKGHRKCLILWHRIQEWADIILNFVKDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG ESRGTEL GIDRTILMRALK LEQKGKLAIFKG+S DDEGIKFS+
Sbjct: 121 EDSVMTVEEIRSGTESRGTELSGIDRTILMRALKLLEQKGKLAIFKGTSADDEGIKFSI 179

BLAST of Cp4.1LG08g08850 vs. NCBI nr
Match: gi|1009174401|ref|XP_015868328.1| (PREDICTED: vacuolar protein sorting-associated protein 25 [Ziziphus jujuba])

HSP 1 Score: 339.3 bits (869), Expect = 4.0e-90
Identity = 162/179 (90.50%), Postives = 172/179 (96.09%), Query Frame = 1

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQ+QLWKELILDYCRTQKIFVIGLEEEFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQVQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SN VIER+LSHEAR  FLSALVS+GRAEWLDKGHRKCLILWHRIQ+WADIIL+FVK+NGL
Sbjct: 61  SNPVIERTLSHEAREAFLSALVSEGRAEWLDKGHRKCLILWHRIQEWADIILNFVKDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG ESRGTEL GIDRTILMRALK LEQKGKLAIFKG+S DDEG+KFS+
Sbjct: 121 EDSVMTVEEIRSGTESRGTELSGIDRTILMRALKLLEQKGKLAIFKGTSADDEGVKFSI 179

BLAST of Cp4.1LG08g08850 vs. NCBI nr
Match: gi|566146665|ref|XP_006368343.1| (hypothetical protein POPTR_0001s01810g [Populus trichocarpa])

HSP 1 Score: 334.0 bits (855), Expect = 1.7e-88
Identity = 159/179 (88.83%), Postives = 171/179 (95.53%), Query Frame = 1

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLP FFNYPPYFTLQPV+DTREKQ+QLWKELILDYCRTQKIFVIGLEEEFPLF
Sbjct: 1   MQKLGDFKLPQFFNYPPYFTLQPVRDTREKQVQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SN +IERSLS+EAR  FLSALVS+GRAEWLD+GHRKCLILWHRIQDWADI+LHFV++NG 
Sbjct: 61  SNHLIERSLSNEAREAFLSALVSEGRAEWLDRGHRKCLILWHRIQDWADILLHFVRDNGF 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIR+GVESRGTEL GIDRTILMRALK LE KGKLAIFKG+STDDEG+KFSV
Sbjct: 121 EDSVMTVEEIRTGVESRGTELHGIDRTILMRALKLLEHKGKLAIFKGTSTDDEGVKFSV 179

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
VPS25_ARATH2.4e-8784.92Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana GN=VPS25 ... [more]
VPS25_XENLA1.8e-3441.04Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis GN=vps25 PE=2 S... [more]
VPS25_DANRE2.0e-3340.46Vacuolar protein-sorting-associated protein 25 OS=Danio rerio GN=vps25 PE=2 SV=2[more]
VPS25_DICDI6.0e-3036.17Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum GN=vp... [more]
VPS25_MOUSE5.1e-2938.15Vacuolar protein-sorting-associated protein 25 OS=Mus musculus GN=Vps25 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0L1R7_CUCSA6.3e-9596.09Uncharacterized protein OS=Cucumis sativus GN=Csa_3G006740 PE=4 SV=1[more]
B9MU83_POPTR1.2e-8888.83Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s01810g PE=4 SV=1[more]
A0A067JBX1_JATCU2.0e-8888.83Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21748 PE=4 SV=1[more]
A0A061G5Y1_THECC2.0e-8888.27E2F/DP family winged-helix DNA-binding domain isoform 1 OS=Theobroma cacao GN=TC... [more]
A0A0D2QMU3_GOSRA9.8e-8887.71Uncharacterized protein OS=Gossypium raimondii GN=B456_003G186000 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G19003.21.4e-8884.92 E2F/DP family winged-helix DNA-binding domain[more]
Match NameE-valueIdentityDescription
gi|659095525|ref|XP_008448627.1|2.4e-9597.21PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis me... [more]
gi|449456737|ref|XP_004146105.1|9.1e-9596.09PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis sa... [more]
gi|1009158488|ref|XP_015897317.1|3.0e-9091.06PREDICTED: vacuolar protein sorting-associated protein 25-like [Ziziphus jujuba][more]
gi|1009174401|ref|XP_015868328.1|4.0e-9090.50PREDICTED: vacuolar protein sorting-associated protein 25 [Ziziphus jujuba][more]
gi|566146665|ref|XP_006368343.1|1.7e-8888.83hypothetical protein POPTR_0001s01810g [Populus trichocarpa][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR014041ESCRT-II_cplx_Vps25-sub_N
IPR011991Winged helix-turn-helix DNA-binding domain
IPR008570ESCRT-II_cplx_Vps25-sub
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0071985 multivesicular body sorting pathway
cellular_component GO:0000814 ESCRT II complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g08850.1Cp4.1LG08g08850.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008570ESCRT-II complex, vps25 subunitPANTHERPTHR13149VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25coord: 4..179
score: 1.2
IPR008570ESCRT-II complex, vps25 subunitPFAMPF05871ESCRT-IIcoord: 13..145
score: 5.9
IPR011991Winged helix-turn-helix DNA-binding domainGENE3DG3DSA:1.10.10.10coord: 104..177
score: 2.6
IPR011991Winged helix-turn-helix DNA-binding domainunknownSSF46785"Winged helix" DNA-binding domaincoord: 7..107
score: 3.02E-32coord: 102..177
score: 1.15
IPR014041ESCRT-II complex, Vps25 subunit, N-terminal winged helixGENE3DG3DSA:1.10.10.570coord: 6..103
score: 3.8

The following gene(s) are paralogous to this gene:

None