CSPI03G01250 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G01250
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Vacuolar protein sorting-associated protein 25
Location	Chr3 : 874611 .. 878797 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCTAATGGGTGAGGTTAGAATCCTAAGCAAACGGTCGAGGATAAACATGTATTTGAATTTTATGAATTTATGGATAAAATATCTAACATCCACAAATATTTTTTAAAGTCACAAAATTGCTTCAACAATGAGAGTTTAGATAAAAGCTTTGAAAAACGAATATTTTCTTCCTTCGTTAATGTTCGTTGTCTACCCGGCAAAAAGAAAACCCTCACCTTAGTCTCCTTCTTCCTCACTCTCGCAACGTTCACTCCCCGCGAATACCGAGTGGCACGAACTCCGGTTGACGGCAATCGAACGGCGGCGACAAACACGATTTAGGTATTATTCATTTGTTCCTTTACCCTCTCTTCACTTCCATCTACGATCGCTCAGCGTTGTTTGATCTGAATATCGAAACACGCCATTTGAAGATTGTGATCACACGGGAACTCTGGAACTTCAGCGTTTTCGACATTCAGCTGAATCAAGATCCTGTGAAGGATTTGGAAGTCGATTCTTGAGTTTTGATTTATCAGTTTATCGTTTTCTCTTACCCTGAAATCAGGCGGTCAATCATTTCGAACACGGCTGGATTTGATTACTGCACTTTTTTAACTTTGAAAATTTGAGTCACTAAATCTCGTTTTCTCTGCGCATCGTTGGTTCTGTGTCTTAATTTTTCATTTTCAATCATTAGATCGATGATTATGATTTTCTTACAGATGTAATAATGGGATTGGGATGACTTTCGAATCATGGAGTGTGTTTTGTTTGTAGTGTTTTGATTAGAGGATTTAAAGTGATCATGGATATTCTAAAACATTTGAGTAGTTTAGGAGTAGTTGTTCAATTTCTTCCAAAAATGTTCTTTACAGCACTTTTTTCAATTTCAAATCCTCCTGAAGTTCTGTGAAATCTTGCCTCGATTAATTTCACAAGCCATTTTACTGTAGGCAATAGCATGCTAGTCATGAAGTTGTTCAGTGTTTCTTCAACAATTTTGCATGTTTAAGTAGTTCCTCAGCTCCTATTTGATAGCCAGTTCAATCTCTTGGTTTGGTTTTTTATCATTGAAAATTAAACTTATAGTCACTTCTTTTACTTTTGGATTTCTTTGGTTTGTTATATTCCTTTTACAAATGTTTTCAAACCCAAGTCAAAGCAAAAACTTGGTTTTGTTTTTTGAATTGAACTACCATGTAAAGAAATGGGGGAGAAATTAGGCGCAATTTTCAAGAACCAAATGGATCAAATGGGGTTCTTTTACATGTTAACTATATAGACCCCAAGAGGTGGTGCTCAATCATGGATCTCTTGTCTTCCAACAAAGTCAGACTTGATATTATGGTCTTTTTCAGTCTTGTATCATGTTACTGTGAAGTTTTCATGCCATTTGATGTTTTTTAGAAATAGCTTCATCAGATGAAATTTATTGTTCGGTTCTCACCACTGATCTGATTCATAGAACACGAAAACTTGATTCTATTTTGACGTGTTCGTTTGCTATCATAATCGGGTACAGGATAAAATTAGAAGAGGAAAGCAACTTGCAGTTCATATAGTTCTTTAATGACAAGGTTTTGTTAGTTTGCAAGTCTCTAGTGTCATCGTATCATTTATGAATCTCTGCGTTCTTTTGTCTCTTCATTTTATCTTTAAATTTCTTTCTGAAATTTCTCAGTTCTAGAGATAAGATGCTTCCATAGAACTCAACTCACTTCCAGAGGACACAATAGAGAAGATGCAGAAATTGGGAGATTTCAAGCTACCGCATTTCTTCAATTATCCTCCATATTTCACGTAATGTTCACCATCTGTTTCATTTATTATTCTTTTTACATCATTATTAGCAACTTGTAAGCTGTGTGAGTCAATTTCTGATGATGTTGATATCTTGGTTTTGTCGACAATTTCCTAAGTGAAGAAAACTAACAGGATACAGTATTTACAACCTATAATTTTCCCACCAGCCTCATATATACGAATGTGCTAGTTGTACCTTACCGCAATGTTGATTGTTTCACTTGTCTTCAGTTGCATATATAACCTTTTTATTGCATGTCATCTCAGTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAACTTTGGAAGGAACTTATCCTTGATTACTGCCGAACGCAAAAGATATTTGTCATTGGACTTGAAGAGGACTTTCCTCTATTTTCAAATTCTGTGATTGAAAGTAAATTTCATGGCCTTTTTTGGTCCTAATTGGGTGTTAAAACCTTTTTGTGTTTTAGACTCAGTTGCATAGCTTTGTATTACTGTTTCAGTTGTTTGATGTATTTTATAGCGTGGTTGTGATGCTACAGGATCTCTCAGTCATGAAGCTAGACAAGCTTTTATCTCAGCTTTAGTTTCAGATGGTTAGGGCCTGCTTGTTTAGTTCGATAATTCTATCTGTTTACAGTTCCCATTCACTTTCATGGGGAAAAAAACTGATACGTGTTCATTTTAATTGTCCTTATAGGTCGTGCAGAATGGCTGGATAAAGGACATAGGAAATGCCTTATTTTGTGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTAAGACTAATTACTTTGGTTAAATTAGTCTCAAATTTTTGGTCTATTTGTCCTTGAACTTTAAAAAATGTCCAATAGGTCTAGTTCAATTCTAAGTTTCATAGATCCTTGAAAATTTTGAAAATGTTAAAAATTATGCGATCCATTAGGTGTAAATTTGGATTTTATGCTAATGGAACTCTAAATCAAAGGTTCGGAGTCTAAATTTGTAAATTTGAAAATTCAAGGTCCAAATAGGCACAAGTTTCAAAAGTTAAATTAAGAGACTGAATTTATTACTTAACCAAATACCATTTTAACTGCCATAGTTATTAGTAAGTTTGAAATGATTTTGAAAGAGTAAAAATTGCTTAATTCCATTGAAAGAAGTAGGTAAAATTCCACAAGTTTTTTGATAGAAATCAGAAGTGCTTTGAAAATTCGTTATACTTAAATTGTTTCTTAATAAATAGGTGAAAAATAATGGACTTGAAGATAGTGTTATGACTGTCGAGGAAATACGTTCAGGAGTTGAATCTAGAGGAACAGGTACTTTCTTTGCTTATGTTCTCAAGGTACTAAATGCCCACCTTTCGATGATAATTAATGGAATGAAATAATGAGATTAAACTGCTGATCATTTCACTTTTTAAATATGTTAATATATTTTATTTATTTTATGTTCTCGTTAGTGCCATTTTATATGTTTCTGAATGTGTTCCCAAAGGTGATATTAAAACATTTATTGCACACTGTATAGCTTTTGATAGAATAAGGATATTCTTGAAACAAGGGACAGTTCATAGGTTATCTTTTTTAGCCAATTTGTGTATACAAATTCATTAGCTCAGCCATAATTGACATGTAATATCTTCATACCTCACATTTGTCGCGTTAAACGTTGTTAGAAACGTAACGTATAAGTAGAGTGATGATGTATATTTGTTATTTGCCACGAGCACAGCGTAGCTATAGTTGACATGTACTTCTCTTTGAGCCCCCCAGGCCATACCTTACTTTTTGTTGAACTAAAACTTAATGTAAAAGATTTTACGTAAACATTTCCTCACCAGATCTGTTGTAGAGACAATACATGCTTGCTTTCTGCAAAACTACGTAGCTGTTTTGTGCTTAAACATTACCATATCTTGAAACAGCAATTTTGCTTCATTATAATTTGTAAGAAAAGAGTTATTTGGTTTTGTGTTTTATAAATCAGAGCTTGAAGGGATTGATCGTACGATATTAATGAGAGCATTGAAGCTACTTGAACAAAAGGGGAAGCTTGCAATTTTCAAAGGTTCTTCGACAGATGATGAAGGCATTAAATTCTCTGTATAAACTACCAATGTTTATTATTCACATTGACTGCCAATCTTTCTTTTTTCAACACTTCTAAATGCAACTTTTCATTTAGTTTCCCCCCAATTCCAACTGGGATTGATGGGATTATAGGTTCTTGGTGCAGTTCATTGAATGTAACCAAGTGGACCTCCCAATTGAACTTAAAAAGACCTAAGCTTGGGAGTGAGGATTAAAATATTGACGTCGATATTGATGTTAAGGTCTTAAACTTACAGATATATTGACCCAGCGACATATATTTTCATCCTTTTTTCGAGAGTGCTTTGGAAAATA

mRNA sequence

ATGCAGAAATTGGGAGATTTCAAGCTACCGCATTTCTTCAATTATCCTCCATATTTCACTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAACTTTGGAAGGAACTTATCCTTGATTACTGCCGAACGCAAAAGATATTTGTCATTGGACTTGAAGAGGACTTTCCTCTATTTTCAAATTCTGTGATTGAAAGATCTCTCAGTCATGAAGCTAGACAAGCTTTTATCTCAGCTTTAGTTTCAGATGGTCGTGCAGAATGGCTGGATAAAGGACATAGGAAATGCCTTATTTTGTGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTGAAAAATAATGGACTTGAAGATAGTGTTATGACTGTCGAGGAAATACGTTCAGGAGTTGAATCTAGAGGAACAGAGCTTGAAGGGATTGATCGTACGATATTAATGAGAGCATTGAAGCTACTTGAACAAAAGGGGAAGCTTGCAATTTTCAAAGGTTCTTCGACAGATGATGAAGGCATTAAATTCTCTGTATAA

Coding sequence (CDS)

ATGCAGAAATTGGGAGATTTCAAGCTACCGCATTTCTTCAATTATCCTCCATATTTCACTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAACTTTGGAAGGAACTTATCCTTGATTACTGCCGAACGCAAAAGATATTTGTCATTGGACTTGAAGAGGACTTTCCTCTATTTTCAAATTCTGTGATTGAAAGATCTCTCAGTCATGAAGCTAGACAAGCTTTTATCTCAGCTTTAGTTTCAGATGGTCGTGCAGAATGGCTGGATAAAGGACATAGGAAATGCCTTATTTTGTGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTGAAAAATAATGGACTTGAAGATAGTGTTATGACTGTCGAGGAAATACGTTCAGGAGTTGAATCTAGAGGAACAGAGCTTGAAGGGATTGATCGTACGATATTAATGAGAGCATTGAAGCTACTTGAACAAAAGGGGAAGCTTGCAATTTTCAAAGGTTCTTCGACAGATGATGAAGGCATTAAATTCTCTGTATAA

Homology

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BLAST of CSPI03G01250 vs. Swiss-Prot
Match: VPS25_ARATH (Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana GN=VPS25 PE=1 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 4.4e-89
Identity = 153/179 (85.47%), Postives = 169/179 (94.41%), Query Frame = 1


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EEDFPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNS I+RSLSHEAR+ F+SA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG ES GTEL+GIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]

BLAST of CSPI03G01250 vs. Swiss-Prot
Match: VPS25_XENLA (Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis GN=vps25 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 2.4e-34
Identity = 69/173 (39.88%), Postives = 106/173 (61.27%), Query Frame = 1


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--DFPLFSNSV 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR  K++ + L E  + PLF+N  
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLSAWSSLVLSYCRQNKLYTMNLMEIQESPLFNNKK 62

Query: 67  IERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           I+R LS E+ Q  +  L   G  EW+DK   + LI+W R  +W  +I  +V  NG+ +SV
Sbjct: 63  IQRKLSLESVQVVLEELRKKGNLEWIDKNKSRFLIMWRRPDEWGKVIYQWVSKNGMTNSV 122

Query: 127 MTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ SG ++ G E  G+D  +L+R+L+ L+Q+ K  I   +  +  G+KF
Sbjct: 123 FTLYELISGDDTEGEEFHGLDEAMLLRSLEALQQEHKAEII--TLNESRGVKF 173

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BLAST of CSPI03G01250 vs. Swiss-Prot
Match: VPS25_DANRE (Vacuolar protein-sorting-associated protein 25 OS=Danio rerio GN=vps25 PE=2 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 1.5e-33
Identity = 69/173 (39.88%), Postives = 107/173 (61.85%), Query Frame = 1


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--DFPLFSNSV 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR +K++ + + E  + P+F+N  
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLAAWCSLVLSYCRHRKLYTLDVLEAQESPVFNNKK 62

Query: 67  IERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           IER LS EA Q     L   G  EWLDK   +CLI+W R ++W  +I  +V  NG+ +SV
Sbjct: 63  IERKLSVEAIQVVFEELRKKGNLEWLDKNKSRCLIMWRRPEEWGKLIYQWVSKNGMVNSV 122

Query: 127 MTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ +G ++   E  G++  +L+R+L+ L+  GK  I   +  D +G+KF
Sbjct: 123 FTLYELANGDDTEKEEFHGLEDWMLLRSLQALQTDGKAEII--TMDDGKGVKF 173

[Close]

BLAST of CSPI03G01250 vs. Swiss-Prot
Match: VPS25_DICDI (Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum GN=vps25 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.9e-31
Identity = 69/188 (36.70%), Postives = 116/188 (61.70%), Query Frame = 1


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFP----LFSN 66
           F+ P +++  P+FT+QP+ +TR+KQ Q+W++LIL YCR  KI+ + + E       LF+N
Sbjct: 6   FQFPPYYHKEPFFTIQPILNTRKKQFQMWQDLILQYCRYYKIYELDINESIKSNSVLFNN 65

Query: 67  SVIERSLSHEARQAFISALVSDGRAEWLDK-------------GHRKCLILWHRIQDWAD 126
             I R LS EA ++ I  ++ +G AEW+DK              + + LI+W +  +WA 
Sbjct: 66  EKINRKLSREALKSIIDDIIENGFAEWVDKEKEKEKEKDKDNNNNNRVLIMWRKPDEWAS 125

Query: 127 IILHFVKNNGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSS 178
           +I  +V + GL ++V+TV EI++G +S+  E   ++ TILM++LK+LE++ K   F  S 
Sbjct: 126 LIYKWVADCGLLNTVLTVWEIQNGDDSKKQEFHQLNTTILMKSLKVLEKQSKCQTF--SQ 185

[Close]

BLAST of CSPI03G01250 vs. Swiss-Prot
Match: VPS25_MOUSE (Vacuolar protein-sorting-associated protein 25 OS=Mus musculus GN=Vps25 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 6.7e-29
Identity = 65/173 (37.57%), Postives = 100/173 (57.80%), Query Frame = 1


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--DFPLFSNSV 66
           F+ P  + +PP+FTLQP  DTR+KQ+  W  L+L +CR  K   + + E  + PLF+N  
Sbjct: 5   FEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVK 64

Query: 67  IERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           ++R L  E+ Q  +  L   G  EWLDK     LI+W R ++W  +I  +V  +G  +SV
Sbjct: 65  LQRKLPVESIQIVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSV 124

Query: 127 MTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ SG ++   E  G+D   L+RAL+ L+Q+ K  I   + +D  G+KF
Sbjct: 125 FTLYELTSGEDTEDEEFHGLDEATLLRALQALQQEHKAEII--TVSDGRGVKF 175

[Close]

BLAST of CSPI03G01250 vs. TrEMBL
Match: A0A0A0L1R7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G006740 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 1.2e-98
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 1


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of CSPI03G01250 vs. TrEMBL
Match: B9MU83_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s01810g PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 2.1e-90
Identity = 160/179 (89.39%), Postives = 174/179 (97.21%), Query Frame = 1


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLP FFNYPPYFTLQPVRDTREKQ+QLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPQFFNYPPYFTLQPVRDTREKQVQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SN +IERSLS+EAR+AF+SALVS+GRAEWLD+GHRKCLILWHRIQDWADI+LHFV++NG 
Sbjct: 61  SNHLIERSLSNEAREAFLSALVSEGRAEWLDRGHRKCLILWHRIQDWADILLHFVRDNGF 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIR+GVESRGTEL GIDRTILMRALKLLE KGKLAIFKG+STDDEG+KFSV
Sbjct: 121 EDSVMTVEEIRTGVESRGTELHGIDRTILMRALKLLEHKGKLAIFKGTSTDDEGVKFSV 179

[Close]

BLAST of CSPI03G01250 vs. TrEMBL
Match: A0A061G5Y1_THECC (E2F/DP family winged-helix DNA-binding domain isoform 1 OS=Theobroma cacao GN=TCM_016610 PE=4 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 2.8e-90
Identity = 159/179 (88.83%), Postives = 173/179 (96.65%), Query Frame = 1


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVR+TREKQ+QLWK+LILDYCRTQKIF+IGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRETREKQVQLWKDLILDYCRTQKIFIIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SN VIER+LSHEAR+AF SALVS+GRAEWLDKGHRKCLILWHRIQDWA IIL+FVK+NG 
Sbjct: 61  SNPVIERTLSHEAREAFFSALVSEGRAEWLDKGHRKCLILWHRIQDWAGIILNFVKDNGF 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG+ESRGTEL GIDRTILMRALK+LEQKGKLAIFKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGIESRGTELHGIDRTILMRALKILEQKGKLAIFKGTSADDEGVKFSV 179

[Close]

BLAST of CSPI03G01250 vs. TrEMBL
Match: A0A067JBX1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21748 PE=4 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 4.7e-90
Identity = 160/179 (89.39%), Postives = 172/179 (96.09%), Query Frame = 1


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLP FFNYPPYFTLQPVRDTREKQ+QLWKELILDYCRTQ+IF I LEE+FPLF
Sbjct: 1   MQKLGDFKLPQFFNYPPYFTLQPVRDTREKQVQLWKELILDYCRTQQIFAIELEEEFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SN VIERSLSHEAR+AF+SALVS+GRAEWLDKGHRKCLILWHRIQDWA+IILHFVK+NGL
Sbjct: 61  SNPVIERSLSHEARKAFLSALVSEGRAEWLDKGHRKCLILWHRIQDWANIILHFVKDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           ED VMT+EEIRSG+ESRGTEL GIDRTILMRALKLLE KGKLAIFKG+STDDEG+KFSV
Sbjct: 121 EDGVMTIEEIRSGIESRGTELHGIDRTILMRALKLLESKGKLAIFKGTSTDDEGVKFSV 179

[Close]

BLAST of CSPI03G01250 vs. TrEMBL
Match: A0A0D2QMU3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_003G186000 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 1.8e-89
Identity = 158/179 (88.27%), Postives = 174/179 (97.21%), Query Frame = 1


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVR+TREKQIQLWKELILDYCRTQKIF++ LEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRETREKQIQLWKELILDYCRTQKIFIVRLEEEFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIER+LSHEAR+AF+SALV++GRAEWLDKG R CLILWHRIQ+WADII++FVK+NG 
Sbjct: 61  SNSVIERTLSHEAREAFLSALVAEGRAEWLDKGRRNCLILWHRIQEWADIIVNFVKDNGF 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG+ESRGTEL GIDRTILMRALKLLEQKGKLAIFKG+STDDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGIESRGTELHGIDRTILMRALKLLEQKGKLAIFKGTSTDDEGVKFSV 179

[Close]

BLAST of CSPI03G01250 vs. TAIR10
Match: AT4G19003.2 (AT4G19003.2 E2F/DP family winged-helix DNA-binding domain)

HSP 1 Score: 328.6 bits (841), Expect = 2.5e-90
Identity = 153/179 (85.47%), Postives = 169/179 (94.41%), Query Frame = 1


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EEDFPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNS I+RSLSHEAR+ F+SA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG ES GTEL+GIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]

BLAST of CSPI03G01250 vs. NCBI nr
Match: gi|449456737|ref|XP_004146105.1| (PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis sativus])

HSP 1 Score: 367.1 bits (941), Expect = 1.8e-98
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 1


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of CSPI03G01250 vs. NCBI nr
Match: gi|659095525|ref|XP_008448627.1| (PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo])

HSP 1 Score: 365.2 bits (936), Expect = 6.8e-98
Identity = 177/179 (98.88%), Postives = 179/179 (100.00%), Query Frame = 1


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEARQAF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTEL+GIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of CSPI03G01250 vs. NCBI nr
Match: gi|1009158488|ref|XP_015897317.1| (PREDICTED: vacuolar protein sorting-associated protein 25-like [Ziziphus jujuba])

HSP 1 Score: 345.5 bits (885), Expect = 5.6e-92
Identity = 164/179 (91.62%), Postives = 175/179 (97.77%), Query Frame = 1


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQ+QLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQVQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SN VIER+LSHEAR+AF+SALVS+GRAEWLDKGHRKCLILWHRIQ+WADIIL+FVK+NGL
Sbjct: 61  SNPVIERTLSHEAREAFLSALVSEGRAEWLDKGHRKCLILWHRIQEWADIILNFVKDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG ESRGTEL GIDRTILMRALKLLEQKGKLAIFKG+S DDEGIKFS+
Sbjct: 121 EDSVMTVEEIRSGTESRGTELSGIDRTILMRALKLLEQKGKLAIFKGTSADDEGIKFSI 179

[Close]

BLAST of CSPI03G01250 vs. NCBI nr
Match: gi|1009174401|ref|XP_015868328.1| (PREDICTED: vacuolar protein sorting-associated protein 25 [Ziziphus jujuba])

HSP 1 Score: 345.1 bits (884), Expect = 7.3e-92
Identity = 163/179 (91.06%), Postives = 175/179 (97.77%), Query Frame = 1


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQ+QLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQVQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SN VIER+LSHEAR+AF+SALVS+GRAEWLDKGHRKCLILWHRIQ+WADIIL+FVK+NGL
Sbjct: 61  SNPVIERTLSHEAREAFLSALVSEGRAEWLDKGHRKCLILWHRIQEWADIILNFVKDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG ESRGTEL GIDRTILMRALKLLEQKGKLAIFKG+S DDEG+KFS+
Sbjct: 121 EDSVMTVEEIRSGTESRGTELSGIDRTILMRALKLLEQKGKLAIFKGTSADDEGVKFSI 179

[Close]

BLAST of CSPI03G01250 vs. NCBI nr
Match: gi|566146665|ref|XP_006368343.1| (hypothetical protein POPTR_0001s01810g [Populus trichocarpa])

HSP 1 Score: 339.7 bits (870), Expect = 3.0e-90
Identity = 160/179 (89.39%), Postives = 174/179 (97.21%), Query Frame = 1


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLP FFNYPPYFTLQPVRDTREKQ+QLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPQFFNYPPYFTLQPVRDTREKQVQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SN +IERSLS+EAR+AF+SALVS+GRAEWLD+GHRKCLILWHRIQDWADI+LHFV++NG 
Sbjct: 61  SNHLIERSLSNEAREAFLSALVSEGRAEWLDRGHRKCLILWHRIQDWADILLHFVRDNGF 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIR+GVESRGTEL GIDRTILMRALKLLE KGKLAIFKG+STDDEG+KFSV
Sbjct: 121 EDSVMTVEEIRTGVESRGTELHGIDRTILMRALKLLEHKGKLAIFKGTSTDDEGVKFSV 179

The following BLAST results are available for this feature:

BLAST of CSPI03G01250 vs. Swiss-Prot


Match Name	E-value	Identity	Description
VPS25_ARATH	4.4e-89	85.47	Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana GN=VPS25 ...	[more]
VPS25_XENLA	2.4e-34	39.88	Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis GN=vps25 PE=2 S...	[more]
VPS25_DANRE	1.5e-33	39.88	Vacuolar protein-sorting-associated protein 25 OS=Danio rerio GN=vps25 PE=2 SV=2	[more]
VPS25_DICDI	1.9e-31	36.70	Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum GN=vp...	[more]
VPS25_MOUSE	6.7e-29	37.57	Vacuolar protein-sorting-associated protein 25 OS=Mus musculus GN=Vps25 PE=1 SV=...	[more]

BLAST of CSPI03G01250 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1R7_CUCSA	1.2e-98	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G006740 PE=4 SV=1	[more]
B9MU83_POPTR	2.1e-90	89.39	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s01810g PE=4 SV=1	[more]
A0A061G5Y1_THECC	2.8e-90	88.83	E2F/DP family winged-helix DNA-binding domain isoform 1 OS=Theobroma cacao GN=TC...	[more]
A0A067JBX1_JATCU	4.7e-90	89.39	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21748 PE=4 SV=1	[more]
A0A0D2QMU3_GOSRA	1.8e-89	88.27	Uncharacterized protein OS=Gossypium raimondii GN=B456_003G186000 PE=4 SV=1	[more]

BLAST of CSPI03G01250 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G19003.2	2.5e-90	85.47	E2F/DP family winged-helix DNA-binding domain	[more]

BLAST of CSPI03G01250 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449456737\|ref\|XP_004146105.1\|	1.8e-98	100.00	PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis sa...	[more]
gi\|659095525\|ref\|XP_008448627.1\|	6.8e-98	98.88	PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis me...	[more]
gi\|1009158488\|ref\|XP_015897317.1\|	5.6e-92	91.62	PREDICTED: vacuolar protein sorting-associated protein 25-like [Ziziphus jujuba]	[more]
gi\|1009174401\|ref\|XP_015868328.1\|	7.3e-92	91.06	PREDICTED: vacuolar protein sorting-associated protein 25 [Ziziphus jujuba]	[more]
gi\|566146665\|ref\|XP_006368343.1\|	3.0e-90	89.39	hypothetical protein POPTR_0001s01810g [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008570	ESCRT-II_cplx_Vps25-sub
IPR011991	Winged helix-turn-helix DNA-binding domain
IPR014041	ESCRT-II_cplx_Vps25-sub_N

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0071985	multivesicular body sorting pathway
cellular_component	GO:0000814	ESCRT II complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G01250.1	CSPI03G01250.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008570	ESCRT-II complex, vps25 subunit	PANTHER	PTHR13149	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25	coord: 4..179 score: 1.5
IPR008570	ESCRT-II complex, vps25 subunit	PFAM	PF05871	ESCRT-II	coord: 13..145 score: 3.2
IPR011991	Winged helix-turn-helix DNA-binding domain	GENE3D	G3DSA:1.10.10.10		coord: 104..177 score: 1.8
IPR011991	Winged helix-turn-helix DNA-binding domain	unknown	SSF46785	"Winged helix" DNA-binding domain	coord: 7..107 score: 1.36E-32coord: 102..177 score: 1.36
IPR014041	ESCRT-II complex, Vps25 subunit, N-terminal winged helix	GENE3D	G3DSA:1.10.10.570		coord: 6..103 score: 2.7
None	No IPR available	PANTHER	PTHR13149:SF0	VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 25	coord: 4..179 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G01250	Csa3G006740	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G01250	MELO3C013816	Melon (DHL92) v3.5.1	cpimeB240
CSPI03G01250	MELO3C013816.2	Melon (DHL92) v3.6.1	cpimedB225
CSPI03G01250	CsaV3_3G001280	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G01250	Bhi07G000828	Wax gourd	cpiwgoB339
CSPI03G01250	Cucsa.322140	Cucumber (Gy14) v1	cgycpiB484
CSPI03G01250	CmaCh06G006200	Cucurbita maxima (Rimu)	cmacpiB829
CSPI03G01250	CmoCh06G006280	Cucurbita moschata (Rifu)	cmocpiB817
CSPI03G01250	Cp4.1LG08g08850	Cucurbita pepo (Zucchini)	cpecpiB865
CSPI03G01250	CsGy3G001160	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G01250	Carg23409	Silver-seed gourd	carcpiB1001

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G01250	Watermelon (97103) v2	cpiwmbB184
CSPI03G01250	Wax gourd	cpiwgoB308
CSPI03G01250	Cucurbita maxima (Rimu)	cmacpiB332
CSPI03G01250	Cucurbita maxima (Rimu)	cmacpiB519
CSPI03G01250	Cucurbita maxima (Rimu)	cmacpiB608
CSPI03G01250	Cucurbita moschata (Rifu)	cmocpiB323
CSPI03G01250	Cucurbita moschata (Rifu)	cmocpiB509
CSPI03G01250	Cucurbita moschata (Rifu)	cmocpiB597
CSPI03G01250	Watermelon (Charleston Gray)	cpiwcgB190
CSPI03G01250	Watermelon (97103) v1	cpiwmB200
CSPI03G01250	Cucurbita pepo (Zucchini)	cpecpiB215
CSPI03G01250	Cucurbita pepo (Zucchini)	cpecpiB245
CSPI03G01250	Cucurbita pepo (Zucchini)	cpecpiB720
CSPI03G01250	Bottle gourd (USVL1VR-Ls)	cpilsiB147
CSPI03G01250	Bottle gourd (USVL1VR-Ls)	cpilsiB227
CSPI03G01250	Silver-seed gourd	carcpiB0818
CSPI03G01250	Silver-seed gourd	carcpiB1085