CsGy1G032920 (gene) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy1G032920
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	Mediator of RNA polymerase II transcription subunit 22b
Location	Chr1 : 32601214 .. 32604166 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAACCCTTCCTGACTCTCTTCTTACCACCTGTTACCACCATCGTGGGACGGATCAAATAAGTTGTTTGAATCTTTTTTGTTAACGTTCTTGTCACATATGTACAACAATTGTGAGAATGGAAAATTGAGAATCGTGTCATGAAAGATTATGTAAGCAGATTTGGCACTTCTCAACTTTTTTTTTTCTCTATAATAAAAGGACATAAAACCCATGGGATTTTCTTCCCTTTTTTCTTGGTGGGCTAAATTTTTATAAGAGCCCATTAACGACCCATATCTAAGCCCATCTCAACTATTGAATTCCACTCGGTGTCTAGGGCTTCGTCATCCTCTGTTCGTGTTGCTTTTTTGTAACGCCGGCAACGCCCATAAGCTCCGACCATCGACACGGCCACACCTTCCACGAACTATCACCGACGACGTTCCACCAGAGTTTTTCTCCATTTTTGTCGACGTGTTATAGACCCACTCTCATTCAACCTCAAATTTGAGTTTCCCATTTCTTAATTGGTTAGTTATATTACATGTTAGAATTTGGGAAAGTCTTGTTGAACTGGTTAGAAAGTGTTTTGAAGATTAGTTTTCTTGATTGGATTTATAGTTTAAATTGTTAATGGTTATGTTTGTCGTGTTAAATTTTTGGGCGTTACATTTTTCTTCCCCAATGGTTCTAATTCCCTTTCGTTTCGATCGCCTTGGCGTCATTGCAGAATTGATTCAATTCCCATTTCTATCTATTATTATGAACAAAGGCATCGGTGGTGGAACTGGCCCCACTGCAGCGGCTGCGGCGGCGGCAGCTCAGAAGCAGAAACTGCTTCTCCAGAGAGTGGAAACTGATATAGCCAATATTGTTGACAATTTCACTCATCTTGTCAACGTCTCTAGAGTAAAACTCTTTCATTTTTATTGTAATTCTCTTTTGTGGTTTATCTGCTTGATCTTCTTGATTTAACAACCTCATGTGTGGAATTCATAATTAACTTACCGTTGTTTCGTCAGGTGAACGATCCACCAGTTAGAAACTCACAAGAATCTTTCATGATGGAAATGCGTGCGGCCAGAATGGTAAGATACTATATTTTCCCTTGATTGTTTACTCTCTTTGTTTGTGATGCTCCTTTTAGGTTGCCATCTGGATAGATAGATAGCTCTTTTGTTCTTCTACAATTGAATGATAATAATCTTATGAAGCCTTGATTTTGTGGGATTGAATGATTTTGCAATAAATTTAGTTGCTTATGCGTCTCTTCCAACATCAGTGAGTATTTCTTCTCCTCTAGCTCCTATTCCAAGAAAATTCTCTTCTTTAGTTGAGGTGGTCTTCATATTCGAATATTTTTGCGGGATCCCAATTGATTCTTCAGAAAAACTTAATTGAGCTTACCTTAGCAATTCATGCAACATCTCGCCTCGATTTGGTTTTAGTTCATGCAATAACAACAGTTGTTTTTTACCAAAAAGTTCATGAAATCATTATGGAGCTTCAATGAAGTCGGTTGGGTTAATGTTGAATCTACTGCGAGATGGAGTGTTTTACTGATTTTGTAGGATGATATGAAGATAAAAGTTTTGGTGTTTCTTAAAGGAGGGTACATTTTCTCAATTAAAATCTATTTTCAGCACAAGTCAATTTGTTGGGTAACAAAGATTTATGGGCTAATTGACTGTAGAGAAATTTTGATAGACCGTCATGTCATAGCATTTGTAGAACAGTTGGTGGTGAGATCTTTTTTAGAGAAAGTATTTTGGCCTGCTTTTGGTAGTTAATTTATTGGTTCTTGTTATCAGCTGCTGTGTATGCTTTTCAAGGTTTCTCTGGACTTGAGAGAATTGGTTTGACGAGTCTTTCTTTGTTTCTTGGCTGTTCTCACTTGATTGCAATTGTTTTAAGGTCTTGTTCTGTTCTCTTTTGGGAGTTTGTATCCTTTGAAGAAGTTATTCCTTGTTGAAAACTCTCATTTTTGGTTTCAAACTATCAAGGTTCTTATATTCATAATGATCATTCACATATAAGATTATAGGCAATAATATATCAGGGATTTATTTTGTCCTTTTCATTATTATTGTGATGGCTACAATTTAGTGTTTCTATACTTGTTTGTTAACAGGTTCAGGCAGCTGATTCTCTGCTGAAGTTGGTTTCAGAACTTAAGCAGACAGCAATATTTTCTGGATTTGCTTCACTAAACGATCATGTGGAGCAAAGAATTGTGGAATTCAATCAGCAGGCACAACAGACTGACCGTCTTTTATCTAGAATTGGTGAAGAAGCTGCTGCCAATTTGAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGAACTGATCAGAACAGCCTGACGTGATTTAGAGTGGAAAGTGGAATTAATGATGCGATGCTGTTGGCTTCTAAACTCAGTTGCTTGCTAACATGGATAATGAAACGTAATATAACACTTCTAACTTTTTCTCTCTCGTTTTACTATAACTTCCTTCTTCACTTGAAAGTGCCTAAAAAAATAGCAAACATGATAGGAAATAAGTAACTCATTCCGTTGTCTGAATTAATTCAGAGAGGACGAAACATGTATGTTGACTTTGGCAGGTAAATTCAGAAATTAATTTTATATAGTTTGATAAGAAGATAATATAATTAATTCATGATGAATGAGGAGGCAACTAAGGAGATGATGAATAAGAAAGTAACAAGGAGAAAACAAAAAAAAGGAAGGAAGCCATTTTGATACGTTGGTAATTTGATGTTCTTCAGAAACTCCTTTATTTGATACAGGAAATCTCACTGTTGTTCATGCTTGCTTCCCTCCTCATTTCCCAACTTCAAATTGGTATATATCTTCTTCTTAATTTTGTGTAAACCTATTTCCTTTTCATGTTTTACTGTCATTTACATCTATATTTCTATGATTAATAAACACACTGCTTATTCTATAACAAACAAAAATTAAA

mRNA sequence

AAAACCCTTCCTGACTCTCTTCTTACCACCTGTTACCACCATCGTGGGACGGATCAAATAAGTTGTTTGAATCTTTTTTGTTAACGTTCTTGTCACATATGTACAACAATTGTGAGAATGGAAAATTGAGAATCGTGTCATGAAAGATTATGTAAGCAGATTTGGCACTTCTCAACTTTTTTTTTTCTCTATAATAAAAGGACATAAAACCCATGGGATTTTCTTCCCTTTTTTCTTGGTGGGCTAAATTTTTATAAGAGCCCATTAACGACCCATATCTAAGCCCATCTCAACTATTGAATTCCACTCGGTGTCTAGGGCTTCGTCATCCTCTGTTCGTGTTGCTTTTTTGTAACGCCGGCAACGCCCATAAGCTCCGACCATCGACACGGCCACACCTTCCACGAACTATCACCGACGACGTTCCACCAGAGTTTTTCTCCATTTTTGTCGACGTGTTATAGACCCACTCTCATTCAACCTCAAATTTGAGTTTCCCATTTCTTAATTGAATTGATTCAATTCCCATTTCTATCTATTATTATGAACAAAGGCATCGGTGGTGGAACTGGCCCCACTGCAGCGGCTGCGGCGGCGGCAGCTCAGAAGCAGAAACTGCTTCTCCAGAGAGTGGAAACTGATATAGCCAATATTGTTGACAATTTCACTCATCTTGTCAACGTCTCTAGAGTGAACGATCCACCAGTTAGAAACTCACAAGAATCTTTCATGATGGAAATGCGTGCGGCCAGAATGGTTCAGGCAGCTGATTCTCTGCTGAAGTTGGTTTCAGAACTTAAGCAGACAGCAATATTTTCTGGATTTGCTTCACTAAACGATCATGTGGAGCAAAGAATTGTGGAATTCAATCAGCAGGCACAACAGACTGACCGTCTTTTATCTAGAATTGGTGAAGAAGCTGCTGCCAATTTGAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGAACTGATCAGAACAGCCTGACGTGATTTAGAGTGGAAAGTGGAATTAATGATGCGATGCTGTTGGCTTCTAAACTCAGTTGCTTGCTAACATGGATAATGAAACGTAATATAACACTTCTAACTTTTTCTCTCTCGTTTTACTATAACTTCCTTCTTCACTTGAAAGTGCCTAAAAAAATAGCAAACATGATAGGAAATAAGTAACTCATTCCGTTGTCTGAATTAATTCAGAGAGGACGAAACATGTATGTTGACTTTGGCAGGTAAATTCAGAAATTAATTTTATATAGTTTGATAAGAAGATAATATAATTAATTCATGATGAATGAGGAGGCAACTAAGGAGATGATGAATAAGAAAGTAACAAGGAGAAAACAAAAAAAAGGAAGGAAGCCATTTTGATACGTTGGTAATTTGATGTTCTTCAGAAACTCCTTTATTTGATACAGGAAATCTCACTGTTGTTCATGCTTGCTTCCCTCCTCATTTCCCAACTTCAAATTGGTATATATCTTCTTCTTAATTTTGTGTAAACCTATTTCCTTTTCATGTTTTACTGTCATTTACATCTATATTTCTATGATTAATAAACACACTGCTTATTCTATAACAAACAAAAATTAAA

Coding sequence (CDS)

ATGAACAAAGGCATCGGTGGTGGAACTGGCCCCACTGCAGCGGCTGCGGCGGCGGCAGCTCAGAAGCAGAAACTGCTTCTCCAGAGAGTGGAAACTGATATAGCCAATATTGTTGACAATTTCACTCATCTTGTCAACGTCTCTAGAGTGAACGATCCACCAGTTAGAAACTCACAAGAATCTTTCATGATGGAAATGCGTGCGGCCAGAATGGTTCAGGCAGCTGATTCTCTGCTGAAGTTGGTTTCAGAACTTAAGCAGACAGCAATATTTTCTGGATTTGCTTCACTAAACGATCATGTGGAGCAAAGAATTGTGGAATTCAATCAGCAGGCACAACAGACTGACCGTCTTTTATCTAGAATTGGTGAAGAAGCTGCTGCCAATTTGAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGAACTGATCAGAACAGCCTGACGTGA

Protein sequence

MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEEAAANLKELESHYYSSTQRTDQNSLT

Homology

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BLAST of CsGy1G032920 vs. NCBI nr
Match: XP_004145443.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like [Cucumis sativus] >KGN66872.1 hypothetical protein Csa_1G701970 [Cucumis sativus])

HSP 1 Score: 236.5 bits (602), Expect = 5.9e-59
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 151
           RIGEEAAANLKELESHYYSSTQRTDQNSLT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 150

[Close]

BLAST of CsGy1G032920 vs. NCBI nr
Match: XP_008459039.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like isoform X2 [Cucumis melo] >XP_008459040.1 PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like isoform X2 [Cucumis melo] >XP_008459041.1 PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like isoform X2 [Cucumis melo])

HSP 1 Score: 233.8 bits (595), Expect = 3.8e-58
Identity = 148/150 (98.67%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQT+RLLS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTERLLS 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 151
           RIGEEAAANLKELESHYYSSTQRTDQN+LT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNNLT 150

[Close]

BLAST of CsGy1G032920 vs. NCBI nr
Match: XP_008459037.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 22a-like isoform X1 [Cucumis melo])

HSP 1 Score: 229.6 bits (584), Expect = 7.2e-57
Identity = 121/123 (98.37%), Postives = 123/123 (100.00%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 87
           QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ
Sbjct: 75  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 134

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEEAAANLKELESHYYSSTQRTDQN 147
           TAIFSGFASLNDHVEQRIVEFNQQAQQT+RLLSRIGEEAAANLKELESHYYSSTQRTDQN
Sbjct: 135 TAIFSGFASLNDHVEQRIVEFNQQAQQTERLLSRIGEEAAANLKELESHYYSSTQRTDQN 194

Query: 148 SLT 151
           +LT
Sbjct: 195 NLT 197

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BLAST of CsGy1G032920 vs. NCBI nr
Match: XP_022155216.1 (mediator of RNA polymerase II transcription subunit 22b-like [Momordica charantia])

HSP 1 Score: 226.9 bits (577), Expect = 4.7e-56
Identity = 142/150 (94.67%), Postives = 145/150 (96.67%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKG+GXXXXXXXXXXXXXXXXXXX  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGVGXXXXXXXXXXXXXXXXXXXLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQA+QTDRLLS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRLLS 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 151
           RIGEEAA +LKELESHYYSSTQRT QN LT
Sbjct: 121 RIGEEAATSLKELESHYYSSTQRTSQNILT 150

[Close]

BLAST of CsGy1G032920 vs. NCBI nr
Match: XP_023545554.1 (mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita pepo subsp. pepo] >XP_023545555.1 mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita pepo subsp. pepo] >XP_023545556.1 mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 225.7 bits (574), Expect = 1.0e-55
Identity = 143/150 (95.33%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           SFMMEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQA+QTDR+LS
Sbjct: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 151
           +IGEEAA  LKELESHYYSSTQRT QNSLT
Sbjct: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150

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BLAST of CsGy1G032920 vs. TAIR10
Match: AT1G07950.1 (Surfeit locus protein 5 subunit 22 of Mediator complex)

HSP 1 Score: 186.4 bits (472), Expect = 1.3e-47
Identity = 94/116 (81.03%), Postives = 111/116 (95.69%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 87
           QRVETDI ++VDNFT +VNVSRV+DPPV+NSQE++MMEMRA+RMVQAADSLLKLVSELKQ
Sbjct: 34  QRVETDITSVVDNFTQIVNVSRVSDPPVKNSQETYMMEMRASRMVQAADSLLKLVSELKQ 93

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEEAAANLKELESHYYSSTQR 144
           TAIFSGFASLNDHVEQRI EF+Q+A++T+RLL+RI ++A+ANLKELESHYYSS QR
Sbjct: 94  TAIFSGFASLNDHVEQRIEEFDQEAEKTNRLLARIADDASANLKELESHYYSSAQR 149

[Close]

BLAST of CsGy1G032920 vs. TAIR10
Match: AT1G16430.1 (Surfeit locus protein 5 subunit 22 of Mediator complex)

HSP 1 Score: 179.1 bits (453), Expect = 2.0e-45
Identity = 88/117 (75.21%), Postives = 111/117 (94.87%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 87
           QRV+TDI ++VDNF  +VNV+RV+DPP++NSQE++MMEMRA+R+VQAADSLLKLVSELKQ
Sbjct: 31  QRVDTDITSVVDNFNQIVNVARVSDPPMKNSQEAYMMEMRASRLVQAADSLLKLVSELKQ 90

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEEAAANLKELESHYYSSTQRT 145
           TAIFSGFASLNDHVEQRI EF+Q+A++T+RLL+RI ++A+A+LKELESHYYSS QR+
Sbjct: 91  TAIFSGFASLNDHVEQRIAEFDQEAEKTNRLLARIADDASASLKELESHYYSSAQRS 147

[Close]

BLAST of CsGy1G032920 vs. Swiss-Prot
Match: sp|Q8LCH5|MD22B_ARATH (Mediator of RNA polymerase II transcription subunit 22b OS=Arabidopsis thaliana OX=3702 GN=MED22B PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 2.3e-46
Identity = 94/116 (81.03%), Postives = 111/116 (95.69%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 87
           QRVETDI ++VDNFT +VNVSRV+DPPV+NSQE++MMEMRA+RMVQAADSLLKLVSELKQ
Sbjct: 34  QRVETDITSVVDNFTQIVNVSRVSDPPVKNSQETYMMEMRASRMVQAADSLLKLVSELKQ 93

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEEAAANLKELESHYYSSTQR 144
           TAIFSGFASLNDHVEQRI EF+Q+A++T+RLL+RI ++A+ANLKELESHYYSS QR
Sbjct: 94  TAIFSGFASLNDHVEQRIEEFDQEAEKTNRLLARIADDASANLKELESHYYSSAQR 149

[Close]

BLAST of CsGy1G032920 vs. Swiss-Prot
Match: sp|Q9SA42|MD22A_ARATH (Mediator of RNA polymerase II transcription subunit 22a OS=Arabidopsis thaliana OX=3702 GN=MED22A PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 3.6e-44
Identity = 88/117 (75.21%), Postives = 111/117 (94.87%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 87
           QRV+TDI ++VDNF  +VNV+RV+DPP++NSQE++MMEMRA+R+VQAADSLLKLVSELKQ
Sbjct: 31  QRVDTDITSVVDNFNQIVNVARVSDPPMKNSQEAYMMEMRASRLVQAADSLLKLVSELKQ 90

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEEAAANLKELESHYYSSTQRT 145
           TAIFSGFASLNDHVEQRI EF+Q+A++T+RLL+RI ++A+A+LKELESHYYSS QR+
Sbjct: 91  TAIFSGFASLNDHVEQRIAEFDQEAEKTNRLLARIADDASASLKELESHYYSSAQRS 147

[Close]

BLAST of CsGy1G032920 vs. Swiss-Prot
Match: sp|Q62276|MED22_MOUSE (Mediator of RNA polymerase II transcription subunit 22 OS=Mus musculus OX=10090 GN=Med22 PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 6.1e-15
Identity = 43/119 (36.13%), Postives = 76/119 (63.87%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDP-----PVRNSQESFMMEMRAARMVQAADSLLKLV 87
           +R++ DI +I+DNFT ++  +++ D        +  Q+++ M +RAA +V+A +SL+KLV
Sbjct: 20  KRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLV 79

Query: 88  SELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEEAAANLKELESHYYSST 142
           S+LKQ  I + F S+N+ ++QR  +     ++ DR L  + +E + +L ELE  YYSS+
Sbjct: 80  SDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRDEVSIDLYELEEEYYSSS 138

[Close]

BLAST of CsGy1G032920 vs. Swiss-Prot
Match: sp|A0JPN6|MED22_RAT (Mediator of RNA polymerase II transcription subunit 22 OS=Rattus norvegicus OX=10116 GN=Med22 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 6.1e-15
Identity = 43/119 (36.13%), Postives = 76/119 (63.87%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDP-----PVRNSQESFMMEMRAARMVQAADSLLKLV 87
           +R++ DI +I+DNFT ++  +++ D        +  Q+++ M +RAA +V+A +SL+KLV
Sbjct: 20  KRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLV 79

Query: 88  SELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEEAAANLKELESHYYSST 142
           S+LKQ  I + F S+N+ ++QR  +     ++ DR L  + +E + +L ELE  YYSS+
Sbjct: 80  SDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRDEVSIDLYELEEEYYSSS 138

[Close]

BLAST of CsGy1G032920 vs. Swiss-Prot
Match: sp|Q5E9K2|MED22_BOVIN (Mediator of RNA polymerase II transcription subunit 22 OS=Bos taurus OX=9913 GN=MED22 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 7.9e-15
Identity = 42/119 (35.29%), Postives = 76/119 (63.87%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDP-----PVRNSQESFMMEMRAARMVQAADSLLKLV 87
           +R++ D+ +I+DNFT ++  +++ D        +  Q+++ M +RAA +V+A +SL+KLV
Sbjct: 20  KRLKDDVKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLV 79

Query: 88  SELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEEAAANLKELESHYYSST 142
           S+LKQ  I + F S+N+ ++QR  +     ++ DR L  + +E + +L ELE  YYSS+
Sbjct: 80  SDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLIALRDEVSIDLYELEEEYYSSS 138

[Close]

BLAST of CsGy1G032920 vs. TrEMBL
Match: tr|A0A0A0M174|A0A0A0M174_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701970 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 3.9e-59
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 151
           RIGEEAAANLKELESHYYSSTQRTDQNSLT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 150

[Close]

BLAST of CsGy1G032920 vs. TrEMBL
Match: tr|A0A1S3C993|A0A1S3C993_CUCME (mediator of RNA polymerase II transcription subunit 22b-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498265 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.5e-58
Identity = 148/150 (98.67%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQT+RLLS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTERLLS 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 151
           RIGEEAAANLKELESHYYSSTQRTDQN+LT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNNLT 150

[Close]

BLAST of CsGy1G032920 vs. TrEMBL
Match: tr|A0A1S3CAG5|A0A1S3CAG5_CUCME (mediator of RNA polymerase II transcription subunit 22a-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498265 PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.8e-57
Identity = 121/123 (98.37%), Postives = 123/123 (100.00%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 87
           QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ
Sbjct: 75  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 134

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEEAAANLKELESHYYSSTQRTDQN 147
           TAIFSGFASLNDHVEQRIVEFNQQAQQT+RLLSRIGEEAAANLKELESHYYSSTQRTDQN
Sbjct: 135 TAIFSGFASLNDHVEQRIVEFNQQAQQTERLLSRIGEEAAANLKELESHYYSSTQRTDQN 194

Query: 148 SLT 151
           +LT
Sbjct: 195 NLT 197

[Close]

BLAST of CsGy1G032920 vs. TrEMBL
Match: tr|A0A1U8JYL3|A0A1U8JYL3_GOSHI (mediator of RNA polymerase II transcription subunit 22b-like OS=Gossypium hirsutum OX=3635 GN=LOC107910490 PE=4 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 9.6e-50
Identity = 107/117 (91.45%), Postives = 113/117 (96.58%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 87
           QRVETDIANIVDNFT LVNV+RVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ
Sbjct: 28  QRVETDIANIVDNFTQLVNVARVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 87

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEEAAANLKELESHYYSSTQRT 145
           TAIFSGFASLNDHVEQR  EFNQQA++TDR+L+RIGEEAAA+LKELESHYYSS QRT
Sbjct: 88  TAIFSGFASLNDHVEQRTTEFNQQAERTDRMLARIGEEAAASLKELESHYYSSAQRT 144

[Close]

BLAST of CsGy1G032920 vs. TrEMBL
Match: tr|A0A2P5S0R1|A0A2P5S0R1_GOSBA (Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_DD10442 PE=4 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 9.6e-50
Identity = 107/117 (91.45%), Postives = 113/117 (96.58%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 87
           QRVETDIANIVDNFT LVNV+RVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ
Sbjct: 28  QRVETDIANIVDNFTQLVNVARVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 87

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEEAAANLKELESHYYSSTQRT 145
           TAIFSGFASLNDHVEQR  EFNQQA++TDR+L+RIGEEAAA+LKELESHYYSS QRT
Sbjct: 88  TAIFSGFASLNDHVEQRTTEFNQQAERTDRMLARIGEEAAASLKELESHYYSSAQRT 144

The following BLAST results are available for this feature:

BLAST of CsGy1G032920 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145443.1	5.9e-59	100.00	PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like [Cucumis...	[more]
XP_008459039.1	3.8e-58	98.67	PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like isoform ...	[more]
XP_008459037.1	7.2e-57	98.37	PREDICTED: mediator of RNA polymerase II transcription subunit 22a-like isoform ...	[more]
XP_022155216.1	4.7e-56	94.67	mediator of RNA polymerase II transcription subunit 22b-like [Momordica charanti...	[more]
XP_023545554.1	1.0e-55	95.33	mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita pepo sub...	[more]

BLAST of CsGy1G032920 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G07950.1	1.3e-47	81.03	Surfeit locus protein 5 subunit 22 of Mediator complex	[more]
AT1G16430.1	2.0e-45	75.21	Surfeit locus protein 5 subunit 22 of Mediator complex	[more]

BLAST of CsGy1G032920 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8LCH5\|MD22B_ARATH	2.3e-46	81.03	Mediator of RNA polymerase II transcription subunit 22b OS=Arabidopsis thaliana ...	[more]
sp\|Q9SA42\|MD22A_ARATH	3.6e-44	75.21	Mediator of RNA polymerase II transcription subunit 22a OS=Arabidopsis thaliana ...	[more]
sp\|Q62276\|MED22_MOUSE	6.1e-15	36.13	Mediator of RNA polymerase II transcription subunit 22 OS=Mus musculus OX=10090 ...	[more]
sp\|A0JPN6\|MED22_RAT	6.1e-15	36.13	Mediator of RNA polymerase II transcription subunit 22 OS=Rattus norvegicus OX=1...	[more]
sp\|Q5E9K2\|MED22_BOVIN	7.9e-15	35.29	Mediator of RNA polymerase II transcription subunit 22 OS=Bos taurus OX=9913 GN=...	[more]

BLAST of CsGy1G032920 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0M174\|A0A0A0M174_CUCSA	3.9e-59	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701970 PE=4 SV=1	[more]
tr\|A0A1S3C993\|A0A1S3C993_CUCME	2.5e-58	98.67	mediator of RNA polymerase II transcription subunit 22b-like isoform X2 OS=Cucum...	[more]
tr\|A0A1S3CAG5\|A0A1S3CAG5_CUCME	4.8e-57	98.37	mediator of RNA polymerase II transcription subunit 22a-like isoform X1 OS=Cucum...	[more]
tr\|A0A1U8JYL3\|A0A1U8JYL3_GOSHI	9.6e-50	91.45	mediator of RNA polymerase II transcription subunit 22b-like OS=Gossypium hirsut...	[more]
tr\|A0A2P5S0R1\|A0A2P5S0R1_GOSBA	9.6e-50	91.45	Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_DD10442 PE=4 SV...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003712	transcription cofactor activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006357	regulation of transcription from RNA polymerase II promoter

Vocabulary: **Cellular Component**
Term	Definition
GO:0016592	mediator complex

Vocabulary: **INTERPRO**
Term	Definition
IPR038427	MED22_sf
IPR009332	Med22

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription from RNA polymerase II promoter
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0001104	RNA polymerase II transcription cofactor activity
molecular_function	GO:0003712	transcription cofactor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy1G032920.1	CsGy1G032920.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009332	Mediator of RNA polymerase II transcription subunit 22	PFAM	PF06179	Med22	coord: 28..127 e-value: 1.9E-28 score: 98.7
IPR009332	Mediator of RNA polymerase II transcription subunit 22	PANTHER	PTHR12434	FAMILY NOT NAMED	coord: 9..145
IPR038427	MED22 superfamily	GENE3D	G3DSA:1.20.58.1600		coord: 13..142 e-value: 2.2E-28 score: 100.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsGy1G032920	Csa1G701970	Cucumber (Chinese Long) v2	cgybcuB002
CsGy1G032920	MELO3C021751	Melon (DHL92) v3.5.1	cgybmeB012
CsGy1G032920	CSPI01G34450	Wild cucumber (PI 183967)	cgybcpiB002
CsGy1G032920	MELO3C021751.2	Melon (DHL92) v3.6.1	cgybmedB011
CsGy1G032920	CsaV3_1G046300	Cucumber (Chinese Long) v3	cgybcucB001

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsGy1G032920	Watermelon (97103) v2	cgybwmbB011
CsGy1G032920	Wax gourd	cgybwgoB028
CsGy1G032920	Cucurbita maxima (Rimu)	cgybcmaB065
CsGy1G032920	Cucurbita maxima (Rimu)	cgybcmaB096
CsGy1G032920	Cucurbita moschata (Rifu)	cgybcmoB061
CsGy1G032920	Cucurbita moschata (Rifu)	cgybcmoB090
CsGy1G032920	Cucurbita pepo (Zucchini)	cgybcpeB008
CsGy1G032920	Cucurbita pepo (Zucchini)	cgybcpeB083
CsGy1G032920	Bottle gourd (USVL1VR-Ls)	cgyblsiB030
CsGy1G032920	Watermelon (Charleston Gray)	cgybwcgB022
CsGy1G032920	Watermelon (97103) v1	cgybwmB050
CsGy1G032920	Watermelon (97103) v1	cgybwmB045