CSPI01G34450 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G34450
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Mediator of RNA polymerase II transcription subunit 22
Location	Chr1 : 29357463 .. 29359800 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATTTTTGTCAGAGCCCATTAACGACCCATATCTAAGCCCATCTCAACTATTGAATTCCAATCGGTGTCTAGGGCTTCGTCATCCTCTGTTCGTGTTGCTTTTTTGTAACGCCGGCGACGCCCATAAGCTCCGACCATCGACACGGCCACACCTTCCACGAACTATCACCGACGACGTTCCACCAGAGTTTTTCTCCATTTTTGTCGACGTGTTATAGACCCACTCTCATTCAACCTCAAATTTGAGTTTCCCATTTCTTAATTGGTTAGTTATATTACATGTTAGAATTTGGGAAAGTCTTGTTGAACTGGTTAGAAAGTGTTTTGAAGATTAGTTTTCTTGATTGGATTTATAGTTTAAATTGTTAATGGTTATGTTTGTCGCGTTAAATTTTTGGGCGTTACATTTTTCTTCCCCAATGGTTCTAATTCCCTTTCGTTTCGATCGCCTGGCGTCGTTGCAGAATTGATTCAATTCCCATTTCTATCTATTATTATGAACAAAGGCATCGGTGGTGGAACTGGCCCCACTGCAGCGGCTGCGGCGGCGGCAGCTCAGAAGCAGAAACTGCTTCTCCAGAGAGTGGAAACTGATATAGCCAATATTGTTGACAATTTCACTCATCTTGTCAACGTCTCTAGAGTAAAACTCTTTCATTTTTATTGTAATTCTCTTTTGTGGTTTATCTGCTTGATCTTCTTGATTTAACAACCTCATGTGTGGAATTCATAATTAACTTACCGTTGTTTCGTCAGGTGAACGATCCACCAGTTAGAAACTCACAAGAATCTTTCATGATGGAAATGCGTGCGGCCAGAATGGTAAGATACTATATTTTCCCTTGATTGTTTACTCTCTTTGTTTGTGATGCTCCTTTTAGGTTGCCATCTGGATAGATAGATAGCTCTTTTGTTCTACAATTGAATGATAATAATCTTATGAAGCCTTGATTTTGTGGGATTGAATGATTTTGCAATAAATTTAGTTGCTTATGCGTCTCTTCCAACATCAGTGAGTATTTCTTCTCCTCTAGCTCCTATTCCAAGAAAATTCTCTTCTTTAGTTGAGGTGGTCTTCATATTCGAATATTTTTGCGGGATCCCAATTGATTCTTCAGAAAAACTTAATTGAGCTTACCTTAGCAATTCATGCAACATCTCGCCTCGATTTGGTTTTAGTTCATGCAATAACAACAGTTGTTTTTTACCAAAAAGTTCATGAAATCATTATGGAGCTTCAATGAAGTCGGTTGGGTTAATGTTGAATCTACTGCGAGATGGAGTGTTTTACTGATTTTGTAGGATGATATGAAGATAAAAGTTTTGGTGTTTCTTAAAGGAGGGTACATTTTCTCAATTAAAATCTATTTTCAGCACAAGTCAATTTGTTGGGTAACAAAGATTTATGGGCTAATTGACTGTAGATAAATTTTGATAGACCGTCATGTCATAGCATTTGTAGAACAGTTGGTGGTGAGATCTTTTTTAGAGAAAGTATTTTGGCCTGCTTTTGGTAGTTAATTTATTGGTTCTTGTTATCAGCTGCTGTGTATGCTTTTCAAGGTTTCTCTGGACTTGAGAGAATTGGTTTGACGAGTCTTTCTTTGTTTCTTGGCTGTTCTCACTTGATTGCAATTGTTTTAAGGTCTTGTTCTGTTCTCTTTTGGGAGTTTGTATCCTTTGAAGAAGTTATTCCTTGTTGAAAACTCTCATTTTTGGTTTCAAACTATCAAGGTTCTTATATTCATAATGATCATTCACATATAAGATTATAGGCAATAATATATCAGGGATTTATTTTGTCCTTTTCATTATTATTGTGATGGCTACAATTTAGTGTTTCTATACTTGTTTGTTAACAGGTTCAGGCAGCTGATTCTCTGCTGAAGTTGGTTTCAGAACTTAAGCAGACAGCAATATTTTCTGGATTTGCTTCACTAAACGATCATGTGGAGCAAAGAATTGTGGAATTCAATCAGCAGGCACAACAGACTGACCGTCTTTTATCTAGAATTGGTGAAGAAGCTGCTGCCAATTTGAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGAACTGATCAGAGCAGCCTGACGTGATTTAGAGTGGAAAGTGGAATTAATGATGTGATACTGTTGGCTTCTAAACTCAGTTGCTTGCTAACATGGATAATGAAACGTAATATAACACTTCTAACTTTTTCTCTCGTTTTACTATAACTTCCTTCTTCACTTGAAAGTGCCTCAAAAAATAGCAAACATGATAGGAAATAAGTAACTCATTCCGTTGTCTGAATTAATTCAGAGAGGACGAAACATGTATGTTGACTTTGGC

mRNA sequence

ATGAACAAAGGCATCGGTGGTGGAACTGGCCCCACTGCAGCGGCTGCGGCGGCGGCAGCTCAGAAGCAGAAACTGCTTCTCCAGAGAGTGGAAACTGATATAGCCAATATTGTTGACAATTTCACTCATCTTGTCAACGTCTCTAGAGTGAACGATCCACCAGTTAGAAACTCACAAGAATCTTTCATGATGGAAATGCGTGCGGCCAGAATGGTTCAGGCAGCTGATTCTCTGCTGAAGTTGGTTTCAGAACTTAAGCAGACAGCAATATTTTCTGGATTTGCTTCACTAAACGATCATGTGGAGCAAAGAATTGTGGAATTCAATCAGCAGGCACAACAGACTGACCGTCTTTTATCTAGAATTGGTGAAGAAGCTGCTGCCAATTTGAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGAACTGATCAGAGCAGCCTGACGTGA

Coding sequence (CDS)

ATGAACAAAGGCATCGGTGGTGGAACTGGCCCCACTGCAGCGGCTGCGGCGGCGGCAGCTCAGAAGCAGAAACTGCTTCTCCAGAGAGTGGAAACTGATATAGCCAATATTGTTGACAATTTCACTCATCTTGTCAACGTCTCTAGAGTGAACGATCCACCAGTTAGAAACTCACAAGAATCTTTCATGATGGAAATGCGTGCGGCCAGAATGGTTCAGGCAGCTGATTCTCTGCTGAAGTTGGTTTCAGAACTTAAGCAGACAGCAATATTTTCTGGATTTGCTTCACTAAACGATCATGTGGAGCAAAGAATTGTGGAATTCAATCAGCAGGCACAACAGACTGACCGTCTTTTATCTAGAATTGGTGAAGAAGCTGCTGCCAATTTGAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGAACTGATCAGAGCAGCCTGACGTGA

Homology

[Close]

BLAST of CSPI01G34450 vs. Swiss-Prot
Match: MD22B_ARATH (Mediator of RNA polymerase II transcription subunit 22b OS=Arabidopsis thaliana GN=MED22B PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 3.2e-56
Identity = 114/140 (81.43%), Postives = 132/140 (94.29%), Query Frame = 1


Query: 4   GIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFM 63
           G GGG+GPTAAAAAAA QKQK LLQRVETDI ++VDNFT +VNVSRV+DPPV+NSQE++M
Sbjct: 10  GSGGGSGPTAAAAAAALQKQKALLQRVETDITSVVDNFTQIVNVSRVSDPPVKNSQETYM 69

Query: 64  MEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIG 123
           MEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRI EF+Q+A++T+RLL+RI 
Sbjct: 70  MEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIEEFDQEAEKTNRLLARIA 129

Query: 124 EEAAANLKELESHYYSSTQR 144
           ++A+ANLKELESHYYSS QR
Sbjct: 130 DDASANLKELESHYYSSAQR 149

[Close]

BLAST of CSPI01G34450 vs. Swiss-Prot
Match: MD22A_ARATH (Mediator of RNA polymerase II transcription subunit 22a OS=Arabidopsis thaliana GN=MED22A PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 1.7e-54
Identity = 111/146 (76.03%), Postives = 135/146 (92.47%), Query Frame = 1


Query: 1   MNKG--IGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNS 60
           MNKG   GGG+GPTAAAAAAA QKQK LLQRV+TDI ++VDNF  +VNV+RV+DPP++NS
Sbjct: 2   MNKGGGSGGGSGPTAAAAAAALQKQKALLQRVDTDITSVVDNFNQIVNVARVSDPPMKNS 61

Query: 61  QESFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRL 120
           QE++MMEMRA+R+VQAADSLLKLVSELKQTAIFSGFASLNDHVEQRI EF+Q+A++T+RL
Sbjct: 62  QEAYMMEMRASRLVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIAEFDQEAEKTNRL 121

Query: 121 LSRIGEEAAANLKELESHYYSSTQRT 145
           L+RI ++A+A+LKELESHYYSS QR+
Sbjct: 122 LARIADDASASLKELESHYYSSAQRS 147

[Close]

BLAST of CSPI01G34450 vs. Swiss-Prot
Match: MED22_MOUSE (Mediator of RNA polymerase II transcription subunit 22 OS=Mus musculus GN=Med22 PE=1 SV=2)

HSP 1 Score: 82.4 bits (202), Expect = 4.6e-15
Identity = 49/137 (35.77%), Postives = 83/137 (60.58%), Query Frame = 1


Query: 14  AAAAAAAQKQKLLLQ----RVETDIANIVDNFTHLVNVSRVNDPP-----VRNSQESFMM 73
           A   A  Q ++ LLQ    R++ DI +I+DNFT ++  +++ D        +  Q+++ M
Sbjct: 2   AQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEM 61

Query: 74  EMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGE 133
            +RAA +V+A +SL+KLVS+LKQ  I + F S+N+ ++QR  +     ++ DR L  + +
Sbjct: 62  HVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRD 121

Query: 134 EAAANLKELESHYYSST 142
           E + +L ELE  YYSS+
Sbjct: 122 EVSIDLYELEEEYYSSS 138

[Close]

BLAST of CSPI01G34450 vs. Swiss-Prot
Match: MED22_RAT (Mediator of RNA polymerase II transcription subunit 22 OS=Rattus norvegicus GN=Med22 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 4.6e-15
Identity = 49/137 (35.77%), Postives = 83/137 (60.58%), Query Frame = 1


Query: 14  AAAAAAAQKQKLLLQ----RVETDIANIVDNFTHLVNVSRVNDPP-----VRNSQESFMM 73
           A   A  Q ++ LLQ    R++ DI +I+DNFT ++  +++ D        +  Q+++ M
Sbjct: 2   AQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEM 61

Query: 74  EMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGE 133
            +RAA +V+A +SL+KLVS+LKQ  I + F S+N+ ++QR  +     ++ DR L  + +
Sbjct: 62  HVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRD 121

Query: 134 EAAANLKELESHYYSST 142
           E + +L ELE  YYSS+
Sbjct: 122 EVSIDLYELEEEYYSSS 138

[Close]

BLAST of CSPI01G34450 vs. Swiss-Prot
Match: MED22_HUMAN (Mediator of RNA polymerase II transcription subunit 22 OS=Homo sapiens GN=MED22 PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 6.0e-15
Identity = 49/137 (35.77%), Postives = 83/137 (60.58%), Query Frame = 1


Query: 14  AAAAAAAQKQKLLLQ----RVETDIANIVDNFTHLVNVSRVNDPP-----VRNSQESFMM 73
           A   A  Q ++ LLQ    R++ DI +I+DNFT ++  +++ D        +  Q+++ M
Sbjct: 2   AQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEM 61

Query: 74  EMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGE 133
            +RAA +V+A +SL+KLVS+LKQ  I + F S+N+ ++QR  +     ++ DR L  + +
Sbjct: 62  HVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRD 121

Query: 134 EAAANLKELESHYYSST 142
           E + +L ELE  YYSS+
Sbjct: 122 EISIDLYELEEEYYSSS 138

[Close]

BLAST of CSPI01G34450 vs. TrEMBL
Match: A0A0A0M174_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G701970 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 8.3e-72
Identity = 149/150 (99.33%), Postives = 150/150 (100.00%), Query Frame = 1


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQSSLT 151
           RIGEEAAANLKELESHYYSSTQRTDQ+SLT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 150

[Close]

BLAST of CSPI01G34450 vs. TrEMBL
Match: A0A0D2N902_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G075400 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 6.6e-61
Identity = 127/148 (85.81%), Postives = 139/148 (93.92%), Query Frame = 1


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKG G GTGPTAAAAAAAAQKQK ++QRVETDIANIVDNFT LVNV+RVNDPPVRNSQE
Sbjct: 1   MNKGTGAGTGPTAAAAAAAAQKQKTMMQRVETDIANIVDNFTQLVNVARVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVE+R  EFNQQA++TD++L+
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVERRTTEFNQQAERTDQMLA 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQSS 149
           RIGEEAAA+LKELESHYYSS QRT  ++
Sbjct: 121 RIGEEAAASLKELESHYYSSAQRTPDTA 148

[Close]

BLAST of CSPI01G34450 vs. TrEMBL
Match: A0A061F1S0_THECC (Surfeit locus protein 5 subunit 22 of Mediator complex OS=Theobroma cacao GN=TCM_025935 PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 9.5e-60
Identity = 126/147 (85.71%), Postives = 139/147 (94.56%), Query Frame = 1


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKG G GTGPTAAAAAAAAQKQK ++QRVETDI NIVDNFT LVNV+RVNDPPVRNSQE
Sbjct: 1   MNKGTGVGTGPTAAAAAAAAQKQKTMMQRVETDIVNIVDNFTQLVNVARVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           +FMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQR VEF+QQA++TDR+L+
Sbjct: 61  AFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRSVEFDQQAEKTDRMLA 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQS 148
           RIGEEAAA+LKELESH+YSS +RT +S
Sbjct: 121 RIGEEAAASLKELESHHYSSARRTAES 147

[Close]

BLAST of CSPI01G34450 vs. TrEMBL
Match: M5W3R2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012665mg PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 1.6e-59
Identity = 126/142 (88.73%), Postives = 137/142 (96.48%), Query Frame = 1


Query: 6   GGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMME 65
           GGG+GPTAAAAAAAAQKQK LLQRVETDIANIVDNF+ LVNVSRVNDPPVRNSQE+FMME
Sbjct: 15  GGGSGPTAAAAAAAAQKQKALLQRVETDIANIVDNFSSLVNVSRVNDPPVRNSQEAFMME 74

Query: 66  MRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIGEE 125
           MRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQA++TD++L+RIGEE
Sbjct: 75  MRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEKTDQMLARIGEE 134

Query: 126 AAANLKELESHYYSSTQRTDQS 148
           AAA+LKELESHYYSS  RT ++
Sbjct: 135 AAASLKELESHYYSSALRTSEN 156

[Close]

BLAST of CSPI01G34450 vs. TrEMBL
Match: D7T100_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0085g01120 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 2.8e-59
Identity = 126/146 (86.30%), Postives = 136/146 (93.15%), Query Frame = 1


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKG G G GPTAAAAAAAAQKQK LLQRVETDIAN+VD+FT LVNV+RVNDPPVRNSQE
Sbjct: 1   MNKGGGAGGGPTAAAAAAAAQKQKTLLQRVETDIANVVDHFTFLVNVARVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           +FMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHV+QR  EFNQQA++TD LL+
Sbjct: 61  AFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVDQREAEFNQQAEKTDHLLA 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQ 147
           RIGEEAAA+LKELESHYYSS QRT +
Sbjct: 121 RIGEEAAASLKELESHYYSSAQRTSE 146

[Close]

BLAST of CSPI01G34450 vs. TAIR10
Match: AT1G07950.1 (AT1G07950.1 Surfeit locus protein 5 subunit 22 of Mediator complex)

HSP 1 Score: 219.2 bits (557), Expect = 1.8e-57
Identity = 114/140 (81.43%), Postives = 132/140 (94.29%), Query Frame = 1


Query: 4   GIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFM 63
           G GGG+GPTAAAAAAA QKQK LLQRVETDI ++VDNFT +VNVSRV+DPPV+NSQE++M
Sbjct: 10  GSGGGSGPTAAAAAAALQKQKALLQRVETDITSVVDNFTQIVNVSRVSDPPVKNSQETYM 69

Query: 64  MEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLSRIG 123
           MEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRI EF+Q+A++T+RLL+RI 
Sbjct: 70  MEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIEEFDQEAEKTNRLLARIA 129

Query: 124 EEAAANLKELESHYYSSTQR 144
           ++A+ANLKELESHYYSS QR
Sbjct: 130 DDASANLKELESHYYSSAQR 149

[Close]

BLAST of CSPI01G34450 vs. TAIR10
Match: AT1G16430.1 (AT1G16430.1 Surfeit locus protein 5 subunit 22 of Mediator complex)

HSP 1 Score: 213.4 bits (542), Expect = 9.7e-56
Identity = 111/146 (76.03%), Postives = 135/146 (92.47%), Query Frame = 1


Query: 1   MNKG--IGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNS 60
           MNKG   GGG+GPTAAAAAAA QKQK LLQRV+TDI ++VDNF  +VNV+RV+DPP++NS
Sbjct: 2   MNKGGGSGGGSGPTAAAAAAALQKQKALLQRVDTDITSVVDNFNQIVNVARVSDPPMKNS 61

Query: 61  QESFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRL 120
           QE++MMEMRA+R+VQAADSLLKLVSELKQTAIFSGFASLNDHVEQRI EF+Q+A++T+RL
Sbjct: 62  QEAYMMEMRASRLVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIAEFDQEAEKTNRL 121

Query: 121 LSRIGEEAAANLKELESHYYSSTQRT 145
           L+RI ++A+A+LKELESHYYSS QR+
Sbjct: 122 LARIADDASASLKELESHYYSSAQRS 147

[Close]

BLAST of CSPI01G34450 vs. NCBI nr
Match: gi|449455405|ref|XP_004145443.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like [Cucumis sativus])

HSP 1 Score: 277.7 bits (709), Expect = 1.2e-71
Identity = 149/150 (99.33%), Postives = 150/150 (100.00%), Query Frame = 1


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQSSLT 151
           RIGEEAAANLKELESHYYSSTQRTDQ+SLT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 150

[Close]

BLAST of CSPI01G34450 vs. NCBI nr
Match: gi|659118278|ref|XP_008459037.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 22a-like isoform X1 [Cucumis melo])

HSP 1 Score: 275.0 bits (702), Expect = 7.7e-71
Identity = 147/150 (98.00%), Postives = 150/150 (100.00%), Query Frame = 1


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 48  MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 107

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQT+RLLS
Sbjct: 108 SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTERLLS 167

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQSSLT 151
           RIGEEAAANLKELESHYYSSTQRTDQ++LT
Sbjct: 168 RIGEEAAANLKELESHYYSSTQRTDQNNLT 197

[Close]

BLAST of CSPI01G34450 vs. NCBI nr
Match: gi|659118280|ref|XP_008459039.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like isoform X2 [Cucumis melo])

HSP 1 Score: 275.0 bits (702), Expect = 7.7e-71
Identity = 147/150 (98.00%), Postives = 150/150 (100.00%), Query Frame = 1


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQT+RLLS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTERLLS 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQSSLT 151
           RIGEEAAANLKELESHYYSSTQRTDQ++LT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNNLT 150

[Close]

BLAST of CSPI01G34450 vs. NCBI nr
Match: gi|823164821|ref|XP_012482354.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like [Gossypium raimondii])

HSP 1 Score: 241.5 bits (615), Expect = 9.5e-61
Identity = 127/148 (85.81%), Postives = 139/148 (93.92%), Query Frame = 1


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKG G GTGPTAAAAAAAAQKQK ++QRVETDIANIVDNFT LVNV+RVNDPPVRNSQE
Sbjct: 1   MNKGTGAGTGPTAAAAAAAAQKQKTMMQRVETDIANIVDNFTQLVNVARVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVE+R  EFNQQA++TD++L+
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVERRTTEFNQQAERTDQMLA 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQSS 149
           RIGEEAAA+LKELESHYYSS QRT  ++
Sbjct: 121 RIGEEAAASLKELESHYYSSAQRTPDTA 148

[Close]

BLAST of CSPI01G34450 vs. NCBI nr
Match: gi|590641021|ref|XP_007030115.1| (Surfeit locus protein 5 subunit 22 of Mediator complex [Theobroma cacao])

HSP 1 Score: 237.7 bits (605), Expect = 1.4e-59
Identity = 126/147 (85.71%), Postives = 139/147 (94.56%), Query Frame = 1


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKG G GTGPTAAAAAAAAQKQK ++QRVETDI NIVDNFT LVNV+RVNDPPVRNSQE
Sbjct: 1   MNKGTGVGTGPTAAAAAAAAQKQKTMMQRVETDIVNIVDNFTQLVNVARVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120
           +FMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQR VEF+QQA++TDR+L+
Sbjct: 61  AFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRSVEFDQQAEKTDRMLA 120

Query: 121 RIGEEAAANLKELESHYYSSTQRTDQS 148
           RIGEEAAA+LKELESH+YSS +RT +S
Sbjct: 121 RIGEEAAASLKELESHHYSSARRTAES 147

The following BLAST results are available for this feature:

BLAST of CSPI01G34450 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MD22B_ARATH	3.2e-56	81.43	Mediator of RNA polymerase II transcription subunit 22b OS=Arabidopsis thaliana ...	[more]
MD22A_ARATH	1.7e-54	76.03	Mediator of RNA polymerase II transcription subunit 22a OS=Arabidopsis thaliana ...	[more]
MED22_MOUSE	4.6e-15	35.77	Mediator of RNA polymerase II transcription subunit 22 OS=Mus musculus GN=Med22 ...	[more]
MED22_RAT	4.6e-15	35.77	Mediator of RNA polymerase II transcription subunit 22 OS=Rattus norvegicus GN=M...	[more]
MED22_HUMAN	6.0e-15	35.77	Mediator of RNA polymerase II transcription subunit 22 OS=Homo sapiens GN=MED22 ...	[more]

BLAST of CSPI01G34450 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M174_CUCSA	8.3e-72	99.33	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G701970 PE=4 SV=1	[more]
A0A0D2N902_GOSRA	6.6e-61	85.81	Uncharacterized protein OS=Gossypium raimondii GN=B456_005G075400 PE=4 SV=1	[more]
A0A061F1S0_THECC	9.5e-60	85.71	Surfeit locus protein 5 subunit 22 of Mediator complex OS=Theobroma cacao GN=TCM...	[more]
M5W3R2_PRUPE	1.6e-59	88.73	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012665mg PE=4 SV=1	[more]
D7T100_VITVI	2.8e-59	86.30	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0085g01120 PE=4 SV=...	[more]

BLAST of CSPI01G34450 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G07950.1	1.8e-57	81.43	Surfeit locus protein 5 subunit 22 of Mediator complex	[more]
AT1G16430.1	9.7e-56	76.03	Surfeit locus protein 5 subunit 22 of Mediator complex	[more]

BLAST of CSPI01G34450 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449455405\|ref\|XP_004145443.1\|	1.2e-71	99.33	PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like [Cucumis...	[more]
gi\|659118278\|ref\|XP_008459037.1\|	7.7e-71	98.00	PREDICTED: mediator of RNA polymerase II transcription subunit 22a-like isoform ...	[more]
gi\|659118280\|ref\|XP_008459039.1\|	7.7e-71	98.00	PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like isoform ...	[more]
gi\|823164821\|ref\|XP_012482354.1\|	9.5e-61	85.81	PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like [Gossypi...	[more]
gi\|590641021\|ref\|XP_007030115.1\|	1.4e-59	85.71	Surfeit locus protein 5 subunit 22 of Mediator complex [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR009332	Med22

Vocabulary: **Molecular Function**
Term	Definition
GO:0001104	RNA polymerase II transcription cofactor activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006357	regulation of transcription from RNA polymerase II promoter

Vocabulary: **Cellular Component**
Term	Definition
GO:0016592	mediator complex

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription from RNA polymerase II promoter
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0001104	RNA polymerase II transcription cofactor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G34450.1	CSPI01G34450.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009332	Mediator of RNA polymerase II transcription subunit 22	PANTHER	PTHR12434	FAMILY NOT NAMED	coord: 1..146 score: 7.4
IPR009332	Mediator of RNA polymerase II transcription subunit 22	PFAM	PF06179	Med22	coord: 28..127 score: 1.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI01G34450	Csa1G701970	Cucumber (Chinese Long) v2	cpicuB001
CSPI01G34450	MELO3C021751	Melon (DHL92) v3.5.1	cpimeB013
CSPI01G34450	MELO3C021751.2	Melon (DHL92) v3.6.1	cpimedB013
CSPI01G34450	CsaV3_1G046300	Cucumber (Chinese Long) v3	cpicucB000
CSPI01G34450	Cucsa.003880	Cucumber (Gy14) v1	cgycpiB036
CSPI01G34450	CsGy1G032920	Cucumber (Gy14) v2	cgybcpiB002

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G34450	Bottle gourd (USVL1VR-Ls)	cpilsiB034
CSPI01G34450	Melon (DHL92) v3.6.1	cpimedB022
CSPI01G34450	Silver-seed gourd	carcpiB0128
CSPI01G34450	Silver-seed gourd	carcpiB0994
CSPI01G34450	Watermelon (97103) v2	cpiwmbB011
CSPI01G34450	Wax gourd	cpiwgoB031
CSPI01G34450	Cucurbita maxima (Rimu)	cmacpiB209
CSPI01G34450	Cucurbita maxima (Rimu)	cmacpiB404
CSPI01G34450	Cucurbita moschata (Rifu)	cmocpiB196
CSPI01G34450	Cucurbita moschata (Rifu)	cmocpiB390
CSPI01G34450	Watermelon (Charleston Gray)	cpiwcgB026
CSPI01G34450	Watermelon (97103) v1	cpiwmB045
CSPI01G34450	Watermelon (97103) v1	cpiwmB053
CSPI01G34450	Cucurbita pepo (Zucchini)	cpecpiB019
CSPI01G34450	Cucurbita pepo (Zucchini)	cpecpiB520