Cp4.1LG20g01260 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG20g01260
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	E3 ubiquitin ligase BIG BROTHER-related protein
Location	Cp4.1LG20 : 762209 .. 765074 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATTATTATTATTATTATTATTATTTGATTTGATAAGGAAGCATCAATTTGGCTTGGAACGCAAAAAAGTGGAAAACCCAACAAAAAAAAAAGAGAGGAAAAAAGTTGTTCACAGGTTCCTGAGCCTGTGAGAGACTGCTCTCTCTATGATGCCGCTTACCCACAAACTCTAACAACAAGACAAACAGAGCCCACCTAACAGCACCACCACCACCACCTCTCTTTTTAATCACTAATTTCTTCCCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCTGCGTTCAAATCTGGGAATGGAACAAAGAGATTCATTAAACTAAAGTTCTTTAAAGTTTATGCTTTTCTCTCTTTCAACTCAATTCATTCCCTTTCACTTTCTGTTCTAAAACCCTTAAATCGCCTCTATTTGTTTGTTTTTCCATTCATTCCTTCTTCTTCTCCAGTTTCCTTGTTTAAGAATCATGCCTGTTGCTCCACAGACTTCTACAATTGGTGAACACATCAATTTGAGAAGACCCAGAAGCCATTTCAGCCAACCCATTTCAGAATCAGATCCAAACCCATCAATCCCTTCGATTATTCAATCCACTCGCTGCAAATCCACCATTTCTTCTCTTCTTCTCTCTACCTTTTCCAATAATTCAACCACCGCCGCCAATGAATGCTTACCCTCTTCCATTGCCACGATCAGGAAGAAGAATAACTTTTCTTCTGCAACTTTCAGGGGACTCGGTTGCACCAACGCTGCTTCTCAGCAGGTATCGGTTCCGGCTGTAATTCGAACTTCAGCGGATTGGGAAAAGAAGAAAACGAGGAAAAAAAAGCAGAAGAACTCCAAGAACAAAACCCATCAAGGAATTGTTGATGCCTCTCATTTTCATCCTAATTCGAGTATCAATTCTGCTAGCTGCTTAGACGCTCAAGATGTTTGGTGTGGCCCTGGAATTGAATTCGCCGCAGATGCGGCTGCTTCTGTGGATTGCGTTGTTGCTAGACGGCATGCTTCTGGAAGGGGAAAAATCGATTTGGAGAAGATTAATCAGAGGGAGGTACTCTGTTTTTACTCTGTTTTAATATATATATTTGAACCTCTTTCTCCTCTGTCGATGTGTCGCACTGAGAAACAAAAACCCCCAGGTGGGTTCTTGTTGTTAAGCATTCGTGTCGATTTCGTAGCTAAAGAAGCATTTGGGTGTTTATTTTATTTGGGCTTTTCTCATTTTTTTTTTTTATAATCGAACTTTATCGTTTTTGTTTCTTTTCTTCAATAGGGTTCCTCGAAATCAGGCTGAACCTGTTCGGCTTCCATTCTTGTAACTTGTTTGATTCCATTATTCTTCGTAAATCTGAGTATTTAATGCAAATGGGTATGCGTTAGATTTGAAATTTTCCGAATGAAATCTTCATTTAGGGCTATAAAACGAGAAATCACCTGTTCGTAGTTGATTATTGGTATCAAACTCTGTTCATTGCGTTTTAACTTCCTCTACTTCTACCATGACAGCGTTCTTCTTTAGGAGGAAGACGAACTGTGAATCCCGAGACCCTTTTATATTTGGATTCTGATTCTGATCTTCCAACTGCTCGAACATTTGAACTGTCTCGGAGTCGATATTATCGCCATGTTCGACATCCATCCCCTGATGGCCTTGCCGAGGTGAGCTATCATTTGTAAATATAAGCTTCAGTTGAAACAACCACTTTGATAGGATTCGTTGCTGTTCTAAAATTATAAGTTTCAGTTGAATTCTATGTTCGTCGTGTTCATCTTGTCATTCTATGTTCTTTTTAAGTACCATAAAGGCATCTCTGTTTCACATTGATCATATGAAACTGATTAGCTCCCCTGATACGTTGTTAGCAGATTATGATGTTTCAGAGCGGGTTGCTGATGGGTGGAAGATTCGACATACACGACCAGTTTAGGGAATTGCGGCTTGATGTCGATAATATGTCGTACGAGGTTAGTAACTTTCTCCAGTTCTTCCATCCTGTTCTTTCTTGCTACAATGTGATACTCACACCTCTTTGAACAGGAGCTGCTTGAGCTCGGTGAAAGGATTGGGCATGTCAGTACTGGGCTGAAAGAAGACGAGATGGGAAGGTGCATTAGAACAATGAAGCCCCTTGCTGTAAATGAGCTAACAACAACTCACTTGCTATCTCAAATGGATCGGAAGTGCAGCATCTGTCAGGTGAAGTAGAAGCGTTTGGTTTTGCTGGATTTGATTACCCCAAATTGTTGACAGCCTACAATTGTTGTGTTATCAAGCAGGAGGAGTATGATCCAGATGATAAAATGGGTAAGCTGGAATGTGGGCATGGGTATCACGTAGAGTGTATAAAGCAGTGGCTTGCGCAGAAGAATGCGTGCCCGGTGTGTAAGACAGCGGCGGTGGGCCGTGGTTGAATGGTTAGTTCATCAATGTGTAGCACTTGTTCGACTCAACCTGCTTGCTTTTCTCCTCCCTATGTGGTGTATAGATTTCATTTGCCTTGTTATATGAGCTAAAGCATTCGTTCATTTGTGTGAAGCTGCTTCCTTTTCTGCTTATAATTGATATGATGGGCGCCATTTTGTTGTTCCTCAGTTGTCTAGAGATTTTATGTAATGATTTTGGATTGACTTGATTGCATATCTTTAAGACCTCAAGGTTCAATGCCTGCCTTTCTTGTGTTCTTCTGGGTACTCAAAAAAATGAAAGAAAGCCCTTATCCAACTTATGAGCATGTAATGTTGTAATCTGATTTCACTATAGCAGAGCTCTGTCTCTCGTGATTGAGTTGACGGTTGAAAGATTCCCATTGTCGAATTTTTTTTACCCGGAAAGTTATATGCTATTTAAGCCGTTTT

mRNA sequence

TATTATTATTATTATTATTATTATTTGATTTGATAAGGAAGCATCAATTTGGCTTGGAACGCAAAAAAGTGGAAAACCCAACAAAAAAAAAAGAGAGGAAAAAAGTTGTTCACAGGTTCCTGAGCCTGTGAGAGACTGCTCTCTCTATGATGCCGCTTACCCACAAACTCTAACAACAAGACAAACAGAGCCCACCTAACAGCACCACCACCACCACCTCTCTTTTTAATCACTAATTTCTTCCCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCTGCGTTCAAATCTGGGAATGGAACAAAGAGATTCATTAAACTAAAGTTCTTTAAAGTTTATGCTTTTCTCTCTTTCAACTCAATTCATTCCCTTTCACTTTCTGTTCTAAAACCCTTAAATCGCCTCTATTTGTTTGTTTTTCCATTCATTCCTTCTTCTTCTCCAGTTTCCTTGTTTAAGAATCATGCCTGTTGCTCCACAGACTTCTACAATTGGTGAACACATCAATTTGAGAAGACCCAGAAGCCATTTCAGCCAACCCATTTCAGAATCAGATCCAAACCCATCAATCCCTTCGATTATTCAATCCACTCGCTGCAAATCCACCATTTCTTCTCTTCTTCTCTCTACCTTTTCCAATAATTCAACCACCGCCGCCAATGAATGCTTACCCTCTTCCATTGCCACGATCAGGAAGAAGAATAACTTTTCTTCTGCAACTTTCAGGGGACTCGGTTGCACCAACGCTGCTTCTCAGCAGGTATCGGTTCCGGCTGTAATTCGAACTTCAGCGGATTGGGAAAAGAAGAAAACGAGGAAAAAAAAGCAGAAGAACTCCAAGAACAAAACCCATCAAGGAATTGTTGATGCCTCTCATTTTCATCCTAATTCGAGTATCAATTCTGCTAGCTGCTTAGACGCTCAAGATGTTTGGTGTGGCCCTGGAATTGAATTCGCCGCAGATGCGGCTGCTTCTGTGGATTGCGTTGTTGCTAGACGGCATGCTTCTGGAAGGGGAAAAATCGATTTGGAGAAGATTAATCAGAGGGAGCGTTCTTCTTTAGGAGGAAGACGAACTGTGAATCCCGAGACCCTTTTATATTTGGATTCTGATTCTGATCTTCCAACTGCTCGAACATTTGAACTGTCTCGGAGTCGATATTATCGCCATGTTCGACATCCATCCCCTGATGGCCTTGCCGAGATTATGATGTTTCAGAGCGGGTTGCTGATGGGTGGAAGATTCGACATACACGACCAGTTTAGGGAATTGCGGCTTGATGTCGATAATATGTCGTACGAGGAGCTGCTTGAGCTCGGTGAAAGGATTGGGCATGTCAGTACTGGGCTGAAAGAAGACGAGATGGGAAGGTGCATTAGAACAATGAAGCCCCTTGCTGTAAATGAGCTAACAACAACTCACTTGCTATCTCAAATGGATCGGAAGTGCAGCATCTGTCAGGAGGAGTATGATCCAGATGATAAAATGGGTAAGCTGGAATGTGGGCATGGGTATCACGTAGAGTGTATAAAGCAGTGGCTTGCGCAGAAGAATGCGTGCCCGGTGTGTAAGACAGCGGCGGTGGGCCGTGGTTGAATGGTTAGTTCATCAATGTGTAGCACTTGTTCGACTCAACCTGCTTGCTTTTCTCCTCCCTATGTGGTGTATAGATTTCATTTGCCTTGTTATATGAGCTAAAGCATTCGTTCATTTGTGTGAAGCTGCTTCCTTTTCTGCTTATAATTGATATGATGGGCGCCATTTTGTTGTTCCTCAGTTGTCTAGAGATTTTATGTAATGATTTTGGATTGACTTGATTGCATATCTTTAAGACCTCAAGGTTCAATGCCTGCCTTTCTTGTGTTCTTCTGGGTACTCAAAAAAATGAAAGAAAGCCCTTATCCAACTTATGAGCATGTAATGTTGTAATCTGATTTCACTATAGCAGAGCTCTGTCTCTCGTGATTGAGTTGACGGTTGAAAGATTCCCATTGTCGAATTTTTTTTACCCGGAAAGTTATATGCTATTTAAGCCGTTTT

Coding sequence (CDS)

ATGCCTGTTGCTCCACAGACTTCTACAATTGGTGAACACATCAATTTGAGAAGACCCAGAAGCCATTTCAGCCAACCCATTTCAGAATCAGATCCAAACCCATCAATCCCTTCGATTATTCAATCCACTCGCTGCAAATCCACCATTTCTTCTCTTCTTCTCTCTACCTTTTCCAATAATTCAACCACCGCCGCCAATGAATGCTTACCCTCTTCCATTGCCACGATCAGGAAGAAGAATAACTTTTCTTCTGCAACTTTCAGGGGACTCGGTTGCACCAACGCTGCTTCTCAGCAGGTATCGGTTCCGGCTGTAATTCGAACTTCAGCGGATTGGGAAAAGAAGAAAACGAGGAAAAAAAAGCAGAAGAACTCCAAGAACAAAACCCATCAAGGAATTGTTGATGCCTCTCATTTTCATCCTAATTCGAGTATCAATTCTGCTAGCTGCTTAGACGCTCAAGATGTTTGGTGTGGCCCTGGAATTGAATTCGCCGCAGATGCGGCTGCTTCTGTGGATTGCGTTGTTGCTAGACGGCATGCTTCTGGAAGGGGAAAAATCGATTTGGAGAAGATTAATCAGAGGGAGCGTTCTTCTTTAGGAGGAAGACGAACTGTGAATCCCGAGACCCTTTTATATTTGGATTCTGATTCTGATCTTCCAACTGCTCGAACATTTGAACTGTCTCGGAGTCGATATTATCGCCATGTTCGACATCCATCCCCTGATGGCCTTGCCGAGATTATGATGTTTCAGAGCGGGTTGCTGATGGGTGGAAGATTCGACATACACGACCAGTTTAGGGAATTGCGGCTTGATGTCGATAATATGTCGTACGAGGAGCTGCTTGAGCTCGGTGAAAGGATTGGGCATGTCAGTACTGGGCTGAAAGAAGACGAGATGGGAAGGTGCATTAGAACAATGAAGCCCCTTGCTGTAAATGAGCTAACAACAACTCACTTGCTATCTCAAATGGATCGGAAGTGCAGCATCTGTCAGGAGGAGTATGATCCAGATGATAAAATGGGTAAGCTGGAATGTGGGCATGGGTATCACGTAGAGTGTATAAAGCAGTGGCTTGCGCAGAAGAATGCGTGCCCGGTGTGTAAGACAGCGGCGGTGGGCCGTGGTTGA

Protein sequence

MPVAPQTSTIGEHINLRRPRSHFSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNNSTTAANECLPSSIATIRKKNNFSSATFRGLGCTNAASQQVSVPAVIRTSADWEKKKTRKKKQKNSKNKTHQGIVDASHFHPNSSINSASCLDAQDVWCGPGIEFAADAAASVDCVVARRHASGRGKIDLEKINQRERSSLGGRRTVNPETLLYLDSDSDLPTARTFELSRSRYYRHVRHPSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVECIKQWLAQKNACPVCKTAAVGRG

Homology

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BLAST of Cp4.1LG20g01260 vs. Swiss-Prot
Match: MBR1_ARATH (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana GN=MBR1 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 3.7e-29
Identity = 67/147 (45.58%), Postives = 88/147 (59.86%), Query Frame = 1


Query: 228 LSRSRYYRHVRHPSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGE 287
           +S  R   H      +   E  M    L+  G  D+HD+ RE+RLDVDNMSYEELL LGE
Sbjct: 557 ISEIRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELLALGE 616

Query: 288 RIGHVSTGLKEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLEC 347
           RIG VSTGL E+ + + ++  K  + +  +    L Q    C ICQEEY   D +G L+C
Sbjct: 617 RIGDVSTGLSEEVILKAMKQHKHTSSSPSSVE--LHQNIEPCCICQEEYVEGDNLGTLKC 676

Query: 348 GHGYHVECIKQWLAQKNACPVCKTAAV 375
           GH +H +CIKQW+  KN CP+CKT A+
Sbjct: 677 GHEFHKDCIKQWVMIKNLCPICKTEAL 701

[Close]

BLAST of Cp4.1LG20g01260 vs. Swiss-Prot
Match: HIP1_ORYSJ (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica GN=HIP1 PE=1 SV=2)

HSP 1 Score: 125.9 bits (315), Expect = 9.1e-28
Identity = 58/117 (49.57%), Postives = 81/117 (69.23%), Query Frame = 1


Query: 259 GRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEMGRCIRTMKPLAVNELTT 318
           G  DIHD+ R++RLD+DNMSYEELL L ERIG+VSTGL E+E+ + ++  K       ++
Sbjct: 555 GGVDIHDRHRDMRLDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRK------FSS 614

Query: 319 THLLSQMDRK-CSICQEEYDPDDKMGKLECGHGYHVECIKQWLAQKNACPVCKTAAV 375
             L + ++ + C ICQEEY   D +G L+CGH +HV C++QWL  KN CP+CK  A+
Sbjct: 615 WRLEASVEEEPCCICQEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICKNTAL 665

[Close]

BLAST of Cp4.1LG20g01260 vs. Swiss-Prot
Match: MBR2_ARATH (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana GN=MBR2 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 3.4e-27
Identity = 60/121 (49.59%), Postives = 81/121 (66.94%), Query Frame = 1


Query: 255 LLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEMGRCIRTMKPLAVN 314
           L+  G  ++HD+ R++RLDVDNMSYEELL LGERIG VSTGL E+ +      +K +  +
Sbjct: 550 LIYQGMAEMHDRHRDMRLDVDNMSYEELLALGERIGDVSTGLSEEVI------LKVMKQH 609

Query: 315 ELTTTHLLSQMDRK-CSICQEEYDPDDKMGKLECGHGYHVECIKQWLAQKNACPVCKTAA 374
           + T++   S  D + C +CQEEY   D +G L CGH +H  C+KQWL  KN CP+CKT A
Sbjct: 610 KHTSSAAGSHQDMEPCCVCQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKTVA 664

[Close]

BLAST of Cp4.1LG20g01260 vs. Swiss-Prot
Match: RHG1A_ARATH (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana GN=RHG1A PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.7e-26
Identity = 59/131 (45.04%), Postives = 82/131 (62.60%), Query Frame = 1


Query: 245 LAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEMGRC 304
           L ++M+    +L  G    HD++R++RLDVDNMSYEELL L ERIG V TG+ E+ +   
Sbjct: 560 LEDVMLLNHSVLFDGATG-HDRYRDMRLDVDNMSYEELLALEERIGDVCTGVNEETISNR 619

Query: 305 IRTMKPLAVNELTTTHLLSQMDRK-CSICQEEYDPDDKMGKLECGHGYHVECIKQWLAQK 364
           ++  K         ++  S  D + C +CQEEY   + MG LECGH +H +CIK+WL QK
Sbjct: 620 LKQRK-------YKSNTKSPQDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQK 679

Query: 365 NACPVCKTAAV 375
           N CP+CKT  +
Sbjct: 680 NLCPICKTTGL 682

[Close]

BLAST of Cp4.1LG20g01260 vs. Swiss-Prot
Match: BB_ARATH (E3 ubiquitin ligase BIG BROTHER OS=Arabidopsis thaliana GN=BB PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.8e-12
Identity = 38/104 (36.54%), Postives = 55/104 (52.88%), Query Frame = 1


Query: 272 LDVDNMSYEELLELGERIGHVSTGLKEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSI 331
           +D D M+YEEL+ELGE +G  S GL ++     I T+ P    +  +     +   +C I
Sbjct: 145 IDPDTMTYEELVELGEAVGTESRGLSQE----LIETL-PTKKYKFGSIFSRKRAGERCVI 204

Query: 332 CQEEYDPDDKMGKLECGHGYHVECIKQWLAQKNACPVCKTAAVG 376
           CQ +Y   ++   L C H YH ECI +WL+    CPVC +   G
Sbjct: 205 CQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFG 243

[Close]

BLAST of Cp4.1LG20g01260 vs. TrEMBL
Match: A0A0A0LQF5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G042600 PE=4 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 4.2e-181
Identity = 329/381 (86.35%), Postives = 349/381 (91.60%), Query Frame = 1


Query: 1   MPVAPQTSTIGEHINLRRPRS---HFSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60
           MPV     T+GEH+ LRRPRS   H +QPISESDPNPSIPSIIQSTRCKSTISSLLLSTF
Sbjct: 1   MPVV----TVGEHMKLRRPRSDLSHLNQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60

Query: 61  SNNSTTAANECLPSSIATI-RKKNNFSSATFRGLGCTNAASQQVSVPAVIRTSADWEKKK 120
           SNN+T+  NE LPSSI T  RKKNNFSSAT RGLGCT AASQQVSVPAVIRTSADWEKKK
Sbjct: 61  SNNTTSGPNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKK 120

Query: 121 TRKKKQKNSKNKTHQGIVDASHFHPNSSINSASCLDAQDVWCGPGIEFAADAAASVDCVV 180
           TRKKKQK+SKNKT QGIVDASHF PNSS+NSASCLDAQDVWCGPGI F+ADAAASVDCVV
Sbjct: 121 TRKKKQKSSKNKTQQGIVDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVV 180

Query: 181 ARRHASGRGKIDLEKINQRERSSLGGRRTVNPETLLYLDSDSDLPTARTFELSRSRYYRH 240
           ARRHASGRGKIDLEKINQRERS L GRRTV+PETLL+LDSDS++PTAR+ ELSRSRYYRH
Sbjct: 181 ARRHASGRGKIDLEKINQRERSCL-GRRTVSPETLLFLDSDSEIPTARSLELSRSRYYRH 240

Query: 241 VRHPSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300
           VRHPSPDGLAEIMMFQS LLMGGRFD+HDQFRELRLDVDNMSYEELLELGERIGHVSTGL
Sbjct: 241 VRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300

Query: 301 KEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVECI 360
           K+DE+GRCIR MKP  VNEL TTHLLSQMDRKCSICQE+Y+PDD+MGKLECGH YH+ CI
Sbjct: 301 KDDEIGRCIRKMKPHVVNEL-TTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCI 360

Query: 361 KQWLAQKNACPVCKTAAVGRG 378
           KQWLAQKN CPVCKTAAVGRG
Sbjct: 361 KQWLAQKNTCPVCKTAAVGRG 375

[Close]

BLAST of Cp4.1LG20g01260 vs. TrEMBL
Match: M5WUI3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007205mg PE=4 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 1.1e-117
Identity = 236/393 (60.05%), Postives = 287/393 (73.03%), Query Frame = 1


Query: 1   MPVAPQTST-IGEHINLRRPRSHFSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSN 60
           MPV  ++ST + EHI  RRPRS FS PIS++DP PSI   IQSTRCKSTISSLLLS+F+ 
Sbjct: 1   MPVVTESSTTVAEHIKWRRPRSQFSPPISDTDPTPSI---IQSTRCKSTISSLLLSSFTT 60

Query: 61  NSTTAANECLPSSIATIRKKNNFSSATFRGLGCTNAASQQVSVPAVIRTSADWEKKKTRK 120
           N+    NE LP+S+   +KK NFSS+TFRGLGC  +ASQQVSVPAVIR SADW+ KK +K
Sbjct: 61  NT----NETLPTSLNN-KKKTNFSSSTFRGLGCAASASQQVSVPAVIRNSADWQGKKVKK 120

Query: 121 KKQK---NSKNKT------------HQGIVDASHFHPNSSINSASCLDAQDVWCGPGIEF 180
           KKQK   N+KN T            HQG VD     P+  +NSA+C+D QDVWCGPGI F
Sbjct: 121 KKQKKNINTKNNTSNDKNEYKDKTQHQGAVDG----PSFGLNSATCMDFQDVWCGPGIGF 180

Query: 181 AADAAASVDCVVARRHASGRGKIDLEKINQRERSSLGGRRTVNPETLLYLDSDSDLPTAR 240
           +A+ A SVDCVVARR+ SGRGKID +K++         RRTV+PET+ +LDS+ D  T+R
Sbjct: 181 SAETAGSVDCVVARRNVSGRGKIDGDKLSSHRERPCLARRTVSPETVSFLDSEPDFVTSR 240

Query: 241 -TFELSRSRYYRHVRHPSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELL 300
              E+   R YRHVRHPSP+GLAEIM+FQS LLMGGR    D+FR+ RLDVDNMSYEELL
Sbjct: 241 PESEVFGGRCYRHVRHPSPEGLAEIMLFQSSLLMGGRL---DRFRDWRLDVDNMSYEELL 300

Query: 301 ELGERIGHVSTGLKEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMG 360
           ELG+RIGHVSTGLKE E+  C+R +K   +++L + H L Q+DRKC ICQEEY+  D +G
Sbjct: 301 ELGDRIGHVSTGLKEHEISCCLRKIKLSMLSDL-SPHSLGQVDRKCIICQEEYEVADDLG 360

Query: 361 KLECGHGYHVECIKQWLAQKNACPVCKTAAVGR 377
            L CGH +H++CIKQWLAQKN CP CK  A+ R
Sbjct: 361 SLHCGHNFHLQCIKQWLAQKNTCPFCKVEAISR 377

[Close]

BLAST of Cp4.1LG20g01260 vs. TrEMBL
Match: W9ST41_9ROSA (E3 ubiquitin ligase BIG BROTHER-related protein OS=Morus notabilis GN=L484_013266 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 6.2e-116
Identity = 239/377 (63.40%), Postives = 289/377 (76.66%), Query Frame = 1


Query: 12  EHINLRRPRSHFSQPISESDPNPS----IPSIIQSTRCKSTISSLLLSTFSNNSTTAANE 71
           E I LRRPRS F++ ISE+DPNPS    IPSIIQSTR KSTISSLLLSTFSN++    NE
Sbjct: 6   ESIELRRPRSQFAELISETDPNPSQTQQIPSIIQSTRGKSTISSLLLSTFSNSN---GNE 65

Query: 72  CLPSSIATIRKKNNFSSATFRGLGCTNAASQQVSVPAVIRTSADWEKKKTRKKKQKNS-- 131
               ++ +++KKNNFSSA+FRG+GC+  +SQQVSVPAVIRTSADWE KK RKKKQKNS  
Sbjct: 66  -TAMAMNSVKKKNNFSSASFRGMGCSAGSSQQVSVPAVIRTSADWEGKKVRKKKQKNSNK 125

Query: 132 KNKTHQGIVDASHFHPNSSINSASCLDAQDVWCGPGIEFAADAAASVDCVVA-RRHASG- 191
           KNK+ +G  D +           +C+D QDVWCGPGI F+ADAAASVDCVVA RR+A+G 
Sbjct: 126 KNKSKEGGKDRNALLDGG--GQQNCVDFQDVWCGPGIGFSADAAASVDCVVATRRNAAGG 185

Query: 192 -RGKIDLEKI--NQRERSSLGGRRTVNPETLLYLDSDSDLPTART-FELSRSRYYRHVRH 251
            RGKID EK+    RERS L  RR V PE   + D++SD  + R   E+  +RY+RHVRH
Sbjct: 186 SRGKIDGEKMTSGHRERSYL-ARRAVIPEPTPFFDTESDFLSERPGSEVFGARYFRHVRH 245

Query: 252 PSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKED 311
           PSP+GLAEIMM  +GLLMGGR D+HD+FR++RLDVDNMSYEELLELGERIG+V+TGLKED
Sbjct: 246 PSPEGLAEIMMLHNGLLMGGRVDMHDRFRDMRLDVDNMSYEELLELGERIGYVNTGLKED 305

Query: 312 EMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVECIKQW 371
           E+GRCIR +K    ++   + L +Q DRKCSICQEEY+ DD +GKL+CGHG+H+ECI+ W
Sbjct: 306 EIGRCIRKIKLSIPSD--WSRLSTQADRKCSICQEEYEADDDIGKLDCGHGFHIECIRHW 365

Query: 372 LAQKNACPVCKTAAVGR 377
           LA KN CPVCK  AV R
Sbjct: 366 LAHKNTCPVCKAEAVAR 373

[Close]

BLAST of Cp4.1LG20g01260 vs. TrEMBL
Match: V7CT75_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G325200g PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 2.3e-115
Identity = 237/390 (60.77%), Postives = 285/390 (73.08%), Query Frame = 1


Query: 1   MPVAPQTST--IGEHINLRRPRSHFSQ----PISESDPNPSIPSIIQSTRCKSTISSLLL 60
           MPV   T+T  + EHI  RRPR+ FS     PI ESDPNP IPSIIQS R KSTISSLLL
Sbjct: 1   MPVVTNTTTPTVAEHIKWRRPRNQFSHHHHHPILESDPNPQIPSIIQSARGKSTISSLLL 60

Query: 61  STFSNNSTTAANECLP-SSIATIRKKNNFSSATFRGLGCTNAASQQVSVPAVIRTSADWE 120
           STFSNN+++++N+    +  A  +KK+NFS+AT RGLGCT  ASQQVSVPAVIR+SADW+
Sbjct: 61  STFSNNTSSSSNDTPTINGTAQSKKKSNFSAATLRGLGCTAGASQQVSVPAVIRSSADWQ 120

Query: 121 KKKTRKKKQKNS----KNKT-HQGIVDASHFHPNSSINSASCLDAQDVWCGPGIEFAADA 180
            KKTRKKK K +    KNKT H GI++ S+           C+D QDVWCGPGI F+AD 
Sbjct: 121 GKKTRKKKHKRATSGGKNKTFHGGILEGSN---------PGCVDFQDVWCGPGIGFSADT 180

Query: 181 AASVDCVVARRHASGRGKIDLEKINQRERSSLGGRRTVNPETLLYLDSDSDLPTARTFEL 240
           AASVDCVVAR++ S RGKID++KI  RERSS  GRRT   ET  +LD+DSD+ T R+   
Sbjct: 181 AASVDCVVARKNVSARGKIDVDKITHRERSSYAGRRT---ETYTFLDTDSDIFTPRSASD 240

Query: 241 S--RSRYYRHVRHPSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELG 300
           S   + YYRHVR PS DG AEIMM Q  LLMGG+ + HDQFR+ RLDVDNMSYE+LLELG
Sbjct: 241 SYGTATYYRHVRDPSSDGFAEIMMLQGSLLMGGQLNSHDQFRDWRLDVDNMSYEQLLELG 300

Query: 301 ERIGHVSTGLKEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLE 360
           ERIGHV+TGLKEDEMGR IR  + L + + T+ H   Q+D++CSICQEEY+  D++G+L 
Sbjct: 301 ERIGHVNTGLKEDEMGRNIRKAR-LQIWDDTSKH---QIDKECSICQEEYEAGDELGRLN 360

Query: 361 CGHGYHVECIKQWLAQKNACPVCKTAAVGR 377
           C H YH +CIKQWL+QKN CPVCK     R
Sbjct: 361 CEHSYHFQCIKQWLSQKNFCPVCKQQVAAR 374

[Close]

BLAST of Cp4.1LG20g01260 vs. TrEMBL
Match: F6GTP6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_17s0000g03210 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 4.0e-115
Identity = 232/381 (60.89%), Postives = 282/381 (74.02%), Query Frame = 1


Query: 1   MPVAPQTSTIGEHINLRRPRSHFSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNN 60
           MPV  + ST+ EHI LRRPRS   QPI ++DPNP IPSIIQSTRCKS+ISSLLLSTFSN 
Sbjct: 1   MPVVAEGSTVAEHIRLRRPRS---QPIPDTDPNPQIPSIIQSTRCKSSISSLLLSTFSN- 60

Query: 61  STTAANECLPSSIATIRKKNNFSSATFRGLGCTNAASQQVSVPAVIRTSADWEKKKTRKK 120
             T AN    S+  +  +K NF+SA FRGLGC  AAS QVSVPAVIRTSADW+ KK RKK
Sbjct: 61  --TNANTNEGSNNTSGGRKKNFASAKFRGLGC--AASSQVSVPAVIRTSADWQAKKVRKK 120

Query: 121 KQKNSKNKTHQ----GIVDASHFHPNSSINSASCLDAQDVWCGPGIEFAADAAASVDCVV 180
           KQ++ + +  Q    G    +   PN    S  C+   DVWC PGI F+ADAA SVDCVV
Sbjct: 121 KQRSLQQQQQQQKKSGNKGVNAVAPNPPAASVECV-VPDVWCAPGIGFSADAA-SVDCVV 180

Query: 181 ARRHASGRGKIDLEKINQRERSSLGGRRTVNPETLLYLDSDSDLPTART-FELSRSRYYR 240
           ARR ASGRGK+D +KINQRER+S   RR  N E + + DSD  +   R   ++   RYYR
Sbjct: 181 ARRPASGRGKVDGDKINQRERASCPTRRATNSEHISFHDSDPTIGMMRPGSDVFGPRYYR 240

Query: 241 HVRHPSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTG 300
           HVRH SP+GLAEI++F+S  LMGGR + HD++R+ RLDVDNMSYEELLELG+RIG+VSTG
Sbjct: 241 HVRHRSPEGLAEIVLFESNFLMGGRSNGHDRYRDWRLDVDNMSYEELLELGDRIGYVSTG 300

Query: 301 LKEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVEC 360
           L+EDE+GRC+R  K   ++EL + HL +++D KCSICQEEY+ DD+MGKLECGHGYH++C
Sbjct: 301 LQEDEIGRCLRKSKLSILDEL-SAHLPTELDWKCSICQEEYEADDEMGKLECGHGYHIDC 360

Query: 361 IKQWLAQKNACPVCKTAAVGR 377
           IKQWL QKN+CPVCK  A  +
Sbjct: 361 IKQWLGQKNSCPVCKATAAAQ 370

[Close]

BLAST of Cp4.1LG20g01260 vs. TAIR10
Match: AT1G73760.1 (AT1G73760.1 RING/U-box superfamily protein)

HSP 1 Score: 293.1 bits (749), Expect = 2.4e-79
Identity = 178/390 (45.64%), Postives = 239/390 (61.28%), Query Frame = 1


Query: 2   PVAPQTSTIGEHINLRRPRSHFSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNNS 61
           P +  ++TIG+H+ L+RPR+H + P   +   P IP    S   KS +SS  L       
Sbjct: 7   PSSSSSTTIGQHMRLQRPRNHRNLPPISTADEPLIPK--PSRVSKSAMSSFFL------- 66

Query: 62  TTAANECLPSSIATIRKKNNFSSATFRGLGCTNAASQQVSVPAVIRTSADWE----KKKT 121
                  LP    T +KK N  +A+FRGLGCT +ASQQVSVPAVIR+SADW+    K K 
Sbjct: 67  -------LPE---TTKKKPN-GTASFRGLGCTTSASQQVSVPAVIRSSADWDASNFKIKK 126

Query: 122 RKKKQKNSKNKTHQGIVDASHFHPNSSINSASCLDAQDVWCGPGIEFAADAAA--SVDCV 181
            KKK KN  + ++ G          S+ +S +C    DVWCGPG+ F+ DA    S+D V
Sbjct: 127 TKKKNKNKGSSSYNG-GSIKILSEASTSSSVACAAIPDVWCGPGVGFSTDAVVGGSIDTV 186

Query: 182 VA---RRHASGRGKIDLEKINQRER------SSLGGRRTVNPETLLYLDSDSDLPTARTF 241
           V+   RR+   R KID +K N          SSL  RR++N E+  Y DSDS   T+R  
Sbjct: 187 VSDPPRRNIPVRRKIDGDKTNSNSNNHREGSSSLLPRRSLNQESNPYFDSDSSFLTSRAE 246

Query: 242 ELSRSRYYRHVRHPSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELG 301
           +  R  Y+RH+R P PDGLAE+MM Q+G +MGG     DQFR++RL+VDNM+YE+LLELG
Sbjct: 247 QTDR--YHRHLRLPYPDGLAEMMMMQNGFVMGGVLSSFDQFRDMRLNVDNMTYEQLLELG 306

Query: 302 ERIGHVSTGLKEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLE 361
           ERIGHV+TGL E ++  C+R +KP    + TT       DRKC ICQ+EY+  D++G+L 
Sbjct: 307 ERIGHVNTGLTEKQIKSCLRKVKP--CRQDTTV-----ADRKCIICQDEYEAKDEVGELR 366

Query: 362 CGHGYHVECIKQWLAQKNACPVCKTAAVGR 377
           CGH +H++C+ QWL +KN+CPVCKT A  +
Sbjct: 367 CGHRFHIDCVNQWLVRKNSCPVCKTMAYNK 366

[Close]

BLAST of Cp4.1LG20g01260 vs. TAIR10
Match: AT1G17970.1 (AT1G17970.1 RING/U-box superfamily protein)

HSP 1 Score: 248.8 bits (634), Expect = 5.2e-66
Identity = 155/381 (40.68%), Postives = 226/381 (59.32%), Query Frame = 1


Query: 7   TSTIGEHINLRRPRSHFSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNNSTTAAN 66
           ++TIGEHI LRR R+   + +  +D +P +  ++      +   +  +S+F    T+++N
Sbjct: 3   STTIGEHIRLRRARNQTIRHLHAADDDPPLSHVVLPISQPNRFCNSAMSSFFPLPTSSSN 62

Query: 67  ECLPSSIATIRKKNNFSSATFRGLGC---TNAASQQVSVPAVIRTSADWEKKKTRKKKQK 126
           E          +K  + +++FRG+GC     AA+Q+VSVP+VIR SAD + +  + KK+K
Sbjct: 63  EST--------RKKPYQTSSFRGMGCYAAAAAAAQEVSVPSVIRYSADLDARIRKDKKKK 122

Query: 127 NSKNKTHQGIVDASHFHPNSSINSASCLDAQDVWCGPGIEFAADAAA--SVDCVVARRHA 186
             K+K  +     S+   +  I S    D  DVWC PG+ F+ DA    SVD    R   
Sbjct: 123 KHKHKKKKKKNKGSYEDGSIRILSEEARDVIDVWCRPGLGFSTDAVIGRSVDPPRGRNIP 182

Query: 187 SGRGKIDLEKINQRER--SSLGGRRTVNPETLLY--LDSDSDLPTARTFE--LSRSRYYR 246
           S R KID++  N      SS+   R +N ET  +   +SDS   T+   E  +  SR   
Sbjct: 183 SSRRKIDVDNNNYNHTLGSSVLPIRFLNQETHSHDIFNSDSTFVTSSRAEPTMLSSRCRG 242

Query: 247 HVRHPSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTG 306
           H+    PD L E+ M Q+G +MG   D  D + ELRLDVD+MSYE+LLELG+RIG+V+TG
Sbjct: 243 HLPRSYPDDLTEMRMLQNGFVMGRITDSRDNYHELRLDVDSMSYEQLLELGDRIGYVNTG 302

Query: 307 LKEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVEC 366
           LKE E+ RC+  +KP      + +H L  +DRKCSICQ+EY+ +D++G+L CGH +HV C
Sbjct: 303 LKESEIHRCLGKIKP------SVSHTL--VDRKCSICQDEYEREDEVGELNCGHSFHVHC 362

Query: 367 IKQWLAQKNACPVCKTAAVGR 377
           +KQWL++KNACPVCK AA G+
Sbjct: 363 VKQWLSRKNACPVCKKAAYGK 367

[Close]

BLAST of Cp4.1LG20g01260 vs. TAIR10
Match: AT4G31450.1 (AT4G31450.1 RING/U-box superfamily protein)

HSP 1 Score: 142.9 bits (359), Expect = 4.0e-34
Identity = 65/131 (49.62%), Postives = 94/131 (71.76%), Query Frame = 1


Query: 247 EIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEMGRCIR 306
           ++++ ++GLL+GG    HDQ R++RLD+DNMSYEELL L ERIG VST L E+ + +C++
Sbjct: 363 DLLIMETGLLLGG-LSFHDQHRDMRLDIDNMSYEELLALEERIGTVSTALTEEAISKCLK 422

Query: 307 T----MKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVECIKQWLAQ 366
           T    MKPL+   +T +   ++ D KCSICQEEY   D++G+L C H YHV+C+++WL  
Sbjct: 423 TSIYQMKPLSYGSITKSPSDNKEDAKCSICQEEYTIGDEVGRLHCEHTYHVKCVQEWLRI 482

Query: 367 KNACPVCKTAA 374
           K+ CP+CK  A
Sbjct: 483 KSWCPICKATA 492

[Close]

BLAST of Cp4.1LG20g01260 vs. TAIR10
Match: AT1G45180.1 (AT1G45180.1 RING/U-box superfamily protein)

HSP 1 Score: 134.8 bits (338), Expect = 1.1e-31
Identity = 61/139 (43.88%), Postives = 90/139 (64.75%), Query Frame = 1


Query: 236 HVRHPSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTG 295
           H+R      L ++M+    +++G   DIHD++R++RLDVDNM+YEELL L ERIG V TG
Sbjct: 506 HMRRAGNLRLEDVMLLNQSIMLGAA-DIHDRYRDMRLDVDNMTYEELLSLEERIGDVCTG 565

Query: 296 LKEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVEC 355
           L E+ +   ++  K        ++   SQ    C +CQEEY  ++++G+LECGH +H +C
Sbjct: 566 LNEETISNRLKQQK------YKSSTRSSQEVEPCCVCQEEYKEEEEIGRLECGHDFHSQC 625

Query: 356 IKQWLAQKNACPVCKTAAV 375
           IK+WL QKN CP+CKT  +
Sbjct: 626 IKEWLKQKNLCPICKTTGL 637

[Close]

BLAST of Cp4.1LG20g01260 vs. TAIR10
Match: AT5G10650.1 (AT5G10650.1 RING/U-box superfamily protein)

HSP 1 Score: 132.1 bits (331), Expect = 7.1e-31
Identity = 59/137 (43.07%), Postives = 84/137 (61.31%), Query Frame = 1


Query: 237 VRHPSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 296
           + H       ++   ++ L   G F  +DQ R++RLD+DNMSYEELL LG+++G VST L
Sbjct: 383 IEHDEELTYEQLASIETNLFSSGMFRFYDQHRDMRLDIDNMSYEELLALGDKMGTVSTAL 442

Query: 297 KEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVECI 356
            E+ + R ++       +E  +  L    D KCSICQEEY   D++G + C H YHV C+
Sbjct: 443 SEEALSRSLKQSIYQETDETGSISLYKDDDIKCSICQEEYVDGDELGTIPCQHMYHVSCV 502

Query: 357 KQWLAQKNACPVCKTAA 374
           +QWL  KN CP+CKT+A
Sbjct: 503 QQWLRMKNWCPICKTSA 519

[Close]

BLAST of Cp4.1LG20g01260 vs. NCBI nr
Match: gi|449439429|ref|XP_004137488.1| (PREDICTED: E3 ubiquitin-protein ligase MBR2 isoform X2 [Cucumis sativus])

HSP 1 Score: 642.1 bits (1655), Expect = 6.0e-181
Identity = 329/381 (86.35%), Postives = 349/381 (91.60%), Query Frame = 1


Query: 1   MPVAPQTSTIGEHINLRRPRS---HFSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60
           MPV     T+GEH+ LRRPRS   H +QPISESDPNPSIPSIIQSTRCKSTISSLLLSTF
Sbjct: 1   MPVV----TVGEHMKLRRPRSDLSHLNQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60

Query: 61  SNNSTTAANECLPSSIATI-RKKNNFSSATFRGLGCTNAASQQVSVPAVIRTSADWEKKK 120
           SNN+T+  NE LPSSI T  RKKNNFSSAT RGLGCT AASQQVSVPAVIRTSADWEKKK
Sbjct: 61  SNNTTSGPNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKK 120

Query: 121 TRKKKQKNSKNKTHQGIVDASHFHPNSSINSASCLDAQDVWCGPGIEFAADAAASVDCVV 180
           TRKKKQK+SKNKT QGIVDASHF PNSS+NSASCLDAQDVWCGPGI F+ADAAASVDCVV
Sbjct: 121 TRKKKQKSSKNKTQQGIVDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVV 180

Query: 181 ARRHASGRGKIDLEKINQRERSSLGGRRTVNPETLLYLDSDSDLPTARTFELSRSRYYRH 240
           ARRHASGRGKIDLEKINQRERS L GRRTV+PETLL+LDSDS++PTAR+ ELSRSRYYRH
Sbjct: 181 ARRHASGRGKIDLEKINQRERSCL-GRRTVSPETLLFLDSDSEIPTARSLELSRSRYYRH 240

Query: 241 VRHPSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300
           VRHPSPDGLAEIMMFQS LLMGGRFD+HDQFRELRLDVDNMSYEELLELGERIGHVSTGL
Sbjct: 241 VRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300

Query: 301 KEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVECI 360
           K+DE+GRCIR MKP  VNEL TTHLLSQMDRKCSICQE+Y+PDD+MGKLECGH YH+ CI
Sbjct: 301 KDDEIGRCIRKMKPHVVNEL-TTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCI 360

Query: 361 KQWLAQKNACPVCKTAAVGRG 378
           KQWLAQKN CPVCKTAAVGRG
Sbjct: 361 KQWLAQKNTCPVCKTAAVGRG 375

[Close]

BLAST of Cp4.1LG20g01260 vs. NCBI nr
Match: gi|659066790|ref|XP_008461518.1| (PREDICTED: uncharacterized protein LOC103500009 isoform X2 [Cucumis melo])

HSP 1 Score: 638.3 bits (1645), Expect = 8.7e-180
Identity = 327/381 (85.83%), Postives = 348/381 (91.34%), Query Frame = 1


Query: 1   MPVAPQTSTIGEHINLRRPRS---HFSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60
           MPV     T+GEH+ LRRPRS   H +QPISESDPNPSIPSIIQSTRCKSTISSLLLSTF
Sbjct: 1   MPVV----TVGEHMKLRRPRSDLSHLNQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60

Query: 61  SNNSTTAANECLPSSIATI-RKKNNFSSATFRGLGCTNAASQQVSVPAVIRTSADWEKKK 120
           SNN+T+  NE LPSSI T  RKKNNFSSAT RGLGCT AASQQVSVPAVIRTSADWE KK
Sbjct: 61  SNNTTSGPNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWENKK 120

Query: 121 TRKKKQKNSKNKTHQGIVDASHFHPNSSINSASCLDAQDVWCGPGIEFAADAAASVDCVV 180
           TRKKKQK+SKNKT QG++DASHF PNSS+NSASCLDAQDVWCGPGI F+ADAAASVDCVV
Sbjct: 121 TRKKKQKSSKNKTQQGVLDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVV 180

Query: 181 ARRHASGRGKIDLEKINQRERSSLGGRRTVNPETLLYLDSDSDLPTARTFELSRSRYYRH 240
           ARRHASGRGKIDLEKINQRERS L GRRTV+PETLL+LDSDSDL TAR+ ELSR+RYYRH
Sbjct: 181 ARRHASGRGKIDLEKINQRERSCL-GRRTVSPETLLFLDSDSDLSTARSLELSRTRYYRH 240

Query: 241 VRHPSPDGLAEIMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300
           VRHPSPDGLAEIMMFQS LLMGGRFD+HDQFRELRLDVDNMSYEELLELGERIGHVSTGL
Sbjct: 241 VRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300

Query: 301 KEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVECI 360
           KEDE+GRCIR MKPL VNEL TTHLLSQMDRKCSICQE+Y+ DD+MGKLECGH YH++CI
Sbjct: 301 KEDEIGRCIRKMKPLVVNEL-TTHLLSQMDRKCSICQEDYEADDEMGKLECGHSYHIQCI 360

Query: 361 KQWLAQKNACPVCKTAAVGRG 378
           KQWLAQKN CPVCKTAAVGRG
Sbjct: 361 KQWLAQKNTCPVCKTAAVGRG 375

[Close]

BLAST of Cp4.1LG20g01260 vs. NCBI nr
Match: gi|778657300|ref|XP_011650643.1| (PREDICTED: E3 ubiquitin-protein ligase MBR2 isoform X1 [Cucumis sativus])

HSP 1 Score: 637.5 bits (1643), Expect = 1.5e-179
Identity = 329/382 (86.13%), Postives = 349/382 (91.36%), Query Frame = 1


Query: 1   MPVAPQTSTIGEHINLRRPRS---HFSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60
           MPV     T+GEH+ LRRPRS   H +QPISESDPNPSIPSIIQSTRCKSTISSLLLSTF
Sbjct: 1   MPVV----TVGEHMKLRRPRSDLSHLNQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60

Query: 61  SNNSTTAANECLPSSIATI-RKKNNFSSATFRGLGCTNAASQQVSVPAVIRTSADWEKKK 120
           SNN+T+  NE LPSSI T  RKKNNFSSAT RGLGCT AASQQVSVPAVIRTSADWEKKK
Sbjct: 61  SNNTTSGPNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKK 120

Query: 121 TRKKKQKNSKNKTHQGIVDASHFHPNSSINSASCLDAQDVWCGPGIEFAADAAASVDCVV 180
           TRKKKQK+SKNKT QGIVDASHF PNSS+NSASCLDAQDVWCGPGI F+ADAAASVDCVV
Sbjct: 121 TRKKKQKSSKNKTQQGIVDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVV 180

Query: 181 ARRHASGRGKIDLEKINQRERSSLGGRRTVNPETLLYLDSDSDLPTARTFELSRSRYYRH 240
           ARRHASGRGKIDLEKINQRERS L GRRTV+PETLL+LDSDS++PTAR+ ELSRSRYYRH
Sbjct: 181 ARRHASGRGKIDLEKINQRERSCL-GRRTVSPETLLFLDSDSEIPTARSLELSRSRYYRH 240

Query: 241 VRHPSPDGLAE-IMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTG 300
           VRHPSPDGLAE IMMFQS LLMGGRFD+HDQFRELRLDVDNMSYEELLELGERIGHVSTG
Sbjct: 241 VRHPSPDGLAEQIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTG 300

Query: 301 LKEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVEC 360
           LK+DE+GRCIR MKP  VNEL TTHLLSQMDRKCSICQE+Y+PDD+MGKLECGH YH+ C
Sbjct: 301 LKDDEIGRCIRKMKPHVVNEL-TTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHC 360

Query: 361 IKQWLAQKNACPVCKTAAVGRG 378
           IKQWLAQKN CPVCKTAAVGRG
Sbjct: 361 IKQWLAQKNTCPVCKTAAVGRG 376

[Close]

BLAST of Cp4.1LG20g01260 vs. NCBI nr
Match: gi|659066788|ref|XP_008461442.1| (PREDICTED: uncharacterized protein LOC103500009 isoform X1 [Cucumis melo])

HSP 1 Score: 633.6 bits (1633), Expect = 2.1e-178
Identity = 327/382 (85.60%), Postives = 348/382 (91.10%), Query Frame = 1


Query: 1   MPVAPQTSTIGEHINLRRPRS---HFSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60
           MPV     T+GEH+ LRRPRS   H +QPISESDPNPSIPSIIQSTRCKSTISSLLLSTF
Sbjct: 1   MPVV----TVGEHMKLRRPRSDLSHLNQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60

Query: 61  SNNSTTAANECLPSSIATI-RKKNNFSSATFRGLGCTNAASQQVSVPAVIRTSADWEKKK 120
           SNN+T+  NE LPSSI T  RKKNNFSSAT RGLGCT AASQQVSVPAVIRTSADWE KK
Sbjct: 61  SNNTTSGPNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWENKK 120

Query: 121 TRKKKQKNSKNKTHQGIVDASHFHPNSSINSASCLDAQDVWCGPGIEFAADAAASVDCVV 180
           TRKKKQK+SKNKT QG++DASHF PNSS+NSASCLDAQDVWCGPGI F+ADAAASVDCVV
Sbjct: 121 TRKKKQKSSKNKTQQGVLDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVV 180

Query: 181 ARRHASGRGKIDLEKINQRERSSLGGRRTVNPETLLYLDSDSDLPTARTFELSRSRYYRH 240
           ARRHASGRGKIDLEKINQRERS L GRRTV+PETLL+LDSDSDL TAR+ ELSR+RYYRH
Sbjct: 181 ARRHASGRGKIDLEKINQRERSCL-GRRTVSPETLLFLDSDSDLSTARSLELSRTRYYRH 240

Query: 241 VRHPSPDGLAE-IMMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTG 300
           VRHPSPDGLAE IMMFQS LLMGGRFD+HDQFRELRLDVDNMSYEELLELGERIGHVSTG
Sbjct: 241 VRHPSPDGLAEQIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTG 300

Query: 301 LKEDEMGRCIRTMKPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVEC 360
           LKEDE+GRCIR MKPL VNEL TTHLLSQMDRKCSICQE+Y+ DD+MGKLECGH YH++C
Sbjct: 301 LKEDEIGRCIRKMKPLVVNEL-TTHLLSQMDRKCSICQEDYEADDEMGKLECGHSYHIQC 360

Query: 361 IKQWLAQKNACPVCKTAAVGRG 378
           IKQWLAQKN CPVCKTAAVGRG
Sbjct: 361 IKQWLAQKNTCPVCKTAAVGRG 376

[Close]

BLAST of Cp4.1LG20g01260 vs. NCBI nr
Match: gi|778657304|ref|XP_011650652.1| (PREDICTED: E3 ubiquitin-protein ligase MBR2 isoform X3 [Cucumis sativus])

HSP 1 Score: 626.3 bits (1614), Expect = 3.4e-176
Identity = 322/369 (87.26%), Postives = 341/369 (92.41%), Query Frame = 1


Query: 14  INLRRPRS---HFSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNNSTTAANECLP 73
           + LRRPRS   H +QPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNN+T+  NE LP
Sbjct: 1   MKLRRPRSDLSHLNQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNNTTSGPNESLP 60

Query: 74  SSIATI-RKKNNFSSATFRGLGCTNAASQQVSVPAVIRTSADWEKKKTRKKKQKNSKNKT 133
           SSI T  RKKNNFSSAT RGLGCT AASQQVSVPAVIRTSADWEKKKTRKKKQK+SKNKT
Sbjct: 61  SSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTRKKKQKSSKNKT 120

Query: 134 HQGIVDASHFHPNSSINSASCLDAQDVWCGPGIEFAADAAASVDCVVARRHASGRGKIDL 193
            QGIVDASHF PNSS+NSASCLDAQDVWCGPGI F+ADAAASVDCVVARRHASGRGKIDL
Sbjct: 121 QQGIVDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVVARRHASGRGKIDL 180

Query: 194 EKINQRERSSLGGRRTVNPETLLYLDSDSDLPTARTFELSRSRYYRHVRHPSPDGLAE-I 253
           EKINQRERS L GRRTV+PETLL+LDSDS++PTAR+ ELSRSRYYRHVRHPSPDGLAE I
Sbjct: 181 EKINQRERSCL-GRRTVSPETLLFLDSDSEIPTARSLELSRSRYYRHVRHPSPDGLAEQI 240

Query: 254 MMFQSGLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEMGRCIRTM 313
           MMFQS LLMGGRFD+HDQFRELRLDVDNMSYEELLELGERIGHVSTGLK+DE+GRCIR M
Sbjct: 241 MMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKDDEIGRCIRKM 300

Query: 314 KPLAVNELTTTHLLSQMDRKCSICQEEYDPDDKMGKLECGHGYHVECIKQWLAQKNACPV 373
           KP  VNEL TTHLLSQMDRKCSICQE+Y+PDD+MGKLECGH YH+ CIKQWLAQKN CPV
Sbjct: 301 KPHVVNEL-TTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNTCPV 360

Query: 374 CKTAAVGRG 378
           CKTAAVGRG
Sbjct: 361 CKTAAVGRG 367

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g01260 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MBR1_ARATH	3.7e-29	45.58	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana GN=MBR1 PE=1 SV=1	[more]
HIP1_ORYSJ	9.1e-28	49.57	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica GN=HIP...	[more]
MBR2_ARATH	3.4e-27	49.59	E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana GN=MBR2 PE=1 SV=1	[more]
RHG1A_ARATH	1.7e-26	45.04	Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana GN=RHG1A PE=2...	[more]
BB_ARATH	1.8e-12	36.54	E3 ubiquitin ligase BIG BROTHER OS=Arabidopsis thaliana GN=BB PE=1 SV=1	[more]

BLAST of Cp4.1LG20g01260 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LQF5_CUCSA	4.2e-181	86.35	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G042600 PE=4 SV=1	[more]
M5WUI3_PRUPE	1.1e-117	60.05	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007205mg PE=4 SV=1	[more]
W9ST41_9ROSA	6.2e-116	63.40	E3 ubiquitin ligase BIG BROTHER-related protein OS=Morus notabilis GN=L484_01326...	[more]
V7CT75_PHAVU	2.3e-115	60.77	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G325200g PE=4 SV=1	[more]
F6GTP6_VITVI	4.0e-115	60.89	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_17s0000g03210 PE=4 SV=...	[more]

BLAST of Cp4.1LG20g01260 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G73760.1	2.4e-79	45.64	RING/U-box superfamily protein	[more]
AT1G17970.1	5.2e-66	40.68	RING/U-box superfamily protein	[more]
AT4G31450.1	4.0e-34	49.62	RING/U-box superfamily protein	[more]
AT1G45180.1	1.1e-31	43.88	RING/U-box superfamily protein	[more]
AT5G10650.1	7.1e-31	43.07	RING/U-box superfamily protein	[more]

BLAST of Cp4.1LG20g01260 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449439429\|ref\|XP_004137488.1\|	6.0e-181	86.35	PREDICTED: E3 ubiquitin-protein ligase MBR2 isoform X2 [Cucumis sativus]	[more]
gi\|659066790\|ref\|XP_008461518.1\|	8.7e-180	85.83	PREDICTED: uncharacterized protein LOC103500009 isoform X2 [Cucumis melo]	[more]
gi\|778657300\|ref\|XP_011650643.1\|	1.5e-179	86.13	PREDICTED: E3 ubiquitin-protein ligase MBR2 isoform X1 [Cucumis sativus]	[more]
gi\|659066788\|ref\|XP_008461442.1\|	2.1e-178	85.60	PREDICTED: uncharacterized protein LOC103500009 isoform X1 [Cucumis melo]	[more]
gi\|778657304\|ref\|XP_011650652.1\|	3.4e-176	87.26	PREDICTED: E3 ubiquitin-protein ligase MBR2 isoform X3 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008270	zinc ion binding
GO:0005515	protein binding

Vocabulary: **INTERPRO**
Term	Definition
IPR013083	Znf_RING/FYVE/PHD
IPR001841	Znf_RING

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG20g01260.1	Cp4.1LG20g01260.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 328..370 score: 1.4
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 329..369 score: 1.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 329..370 score: 12
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 326..371 score: 2.8
None	No IPR available	PANTHER	PTHR22937	RING FINGER CONTAINING PROTEIN	coord: 20..374 score: 6.5E
None	No IPR available	PANTHER	PTHR22937:SF18	F6A14.12 PROTEIN-RELATED	coord: 20..374 score: 6.5E
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 315..372 score: 3.53

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG20g01260	Cucsa.121730	Cucumber (Gy14) v1	cgycpeB0308
Cp4.1LG20g01260	CmaCh13G010070	Cucurbita maxima (Rimu)	cmacpeB234
Cp4.1LG20g01260	CmaCh18G000520	Cucurbita maxima (Rimu)	cmacpeB439
Cp4.1LG20g01260	CmoCh18G000420	Cucurbita moschata (Rifu)	cmocpeB404
Cp4.1LG20g01260	CmoCh13G010450	Cucurbita moschata (Rifu)	cmocpeB201
Cp4.1LG20g01260	Cla018476	Watermelon (97103) v1	cpewmB523
Cp4.1LG20g01260	Csa1G042600	Cucumber (Chinese Long) v2	cpecuB505
Cp4.1LG20g01260	MELO3C002110	Melon (DHL92) v3.5.1	cpemeB478
Cp4.1LG20g01260	ClCG04G010890	Watermelon (Charleston Gray)	cpewcgB471
Cp4.1LG20g01260	CSPI01G07000	Wild cucumber (PI 183967)	cpecpiB505
Cp4.1LG20g01260	Lsi02G002120	Bottle gourd (USVL1VR-Ls)	cpelsiB418
Cp4.1LG20g01260	MELO3C002110.2	Melon (DHL92) v3.6.1	cpemedB560
Cp4.1LG20g01260	CsaV3_1G006940	Cucumber (Chinese Long) v3	cpecucB0623
Cp4.1LG20g01260	Bhi08G001053	Wax gourd	cpewgoB0635
Cp4.1LG20g01260	CsGy1G006820	Cucumber (Gy14) v2	cgybcpeB069
Cp4.1LG20g01260	Carg22741	Silver-seed gourd	carcpeB0725
Cp4.1LG20g01260	Carg04830	Silver-seed gourd	carcpeB0544

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG20g01260	Cp4.1LG09g11340	Cucurbita pepo (Zucchini)	cpecpeB049

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG20g01260	Cucurbita pepo (Zucchini)	cpecpeB352
Cp4.1LG20g01260	Cucurbita pepo (Zucchini)	cpecpeB442
Cp4.1LG20g01260	Cucumber (Gy14) v1	cgycpeB0258
Cp4.1LG20g01260	Cucurbita maxima (Rimu)	cmacpeB139
Cp4.1LG20g01260	Cucurbita moschata (Rifu)	cmocpeB116
Cp4.1LG20g01260	Wild cucumber (PI 183967)	cpecpiB509
Cp4.1LG20g01260	Cucumber (Chinese Long) v2	cpecuB509
Cp4.1LG20g01260	Bottle gourd (USVL1VR-Ls)	cpelsiB432
Cp4.1LG20g01260	Watermelon (Charleston Gray)	cpewcgB475
Cp4.1LG20g01260	Watermelon (97103) v1	cpewmB529
Cp4.1LG20g01260	Melon (DHL92) v3.5.1	cpemeB483
Cp4.1LG20g01260	Cucumber (Gy14) v2	cgybcpeB073
Cp4.1LG20g01260	Melon (DHL92) v3.6.1	cpemedB568
Cp4.1LG20g01260	Silver-seed gourd	carcpeB0846
Cp4.1LG20g01260	Silver-seed gourd	carcpeB1104
Cp4.1LG20g01260	Cucumber (Chinese Long) v3	cpecucB0627