Cp4.1LG17g10070 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG17g10070
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionrRNA N-glycosidase
LocationCp4.1LG17 : 7685694 .. 7686992 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACTCTTATAAGAAATGTTTCGTTCTCCTCTCCAACGACACGCGACGACACCCAATTGAATTGTGGTCGTAATTAGAAAAAGAAAAATATCCAAAACATTGCCCCATCTATAAAGAGACATCAAGATCCTCATACCACTCAAACCAACCACCAAGACAAAAAGATGAACAAACTCATAGCAATCTCCTCTTTCTTACTAGCCATCTCTCTTATCTCTCCCACTCTCAAGGCTGACATAGCCTTGGATTTGTCCACAGCTACCAAAGCATCCTATTCAGCTTTCATAACCCACATCAGGAATGCTCTTCCAACCAAAGCAACAGTGTGCAACATACCCCTGCTGCCTTCCACCCCAACGGGCTTAGCCTCGTTCACATTCTTCACCATCACCAATTATAACTACCAAACCGTCGAGGTCGCTGTGAACGTAACCAACGTCTACATCGTGGCGTATCGTGCCGGTGCAAGCTCCTACTTTTTCGAAGACACTCCAGCTCAAGCCAAACAACTCTTATTTGCAGGTACTCAAAAGGTAACACTTCCTTTTACGGGTAACTATGATAAGCTCCAAAGCATTGTAGGCAAATATAGAGATATGGTTGAGCTTGGAATCCCCGCCTTGAGCACTGCAGTTACCAACATGGTTTACTACAATAAACAACTGACTGCGGCGGCGCTTCTTGTGCTCATTCAGTCCACTGCTGAAGCTGCGAGGTTTAAATATATTGAGCAACAGGTTGCTCAGCATATTAGCGATAAGTTTTATCCAAACCAAGCAGTGATAAGCTTAGAGAACAACTGGGGAGCTCTCTCCAAGCAAATCCAGATAGCAAATAGTACTGGAAGGGGACAATTCCAGAGCCCTGTTGAGCTTATAGGCCCTACTGGCACACGATTTAGTGTAACCAATACGTCCGCTGGGGTTGTTCAAGGCAATATCAAGCTCCTGCTGTACTACAAACTGAGCGTTGCTGATGACAACGACAGCCCGACAAGAATGCCTGACAATATTGTTGAATATGGAGCTCATGATTCTATGTAGCTCTATGGCTTTGTTTGAATATCCACACATAACAATGGATTGAGGCATGTTCATATGTGAATGTGCTTGATTTCATTAAATAATAAATAAGTGGGGATTTTGAAAAATTCGAGTTTTTTACTCCACATTGATTCATAGGGTTTGTGGCTCGAGTCTCTCGAAAAATAGATCAGTATAGTTACTATAGAACAAGTATATGAACGGAATTATACACTTGTACCCTAATGTCACATCCAAAAATGGTTTGATACCCAATT

mRNA sequence

CACTCTTATAAGAAATGTTTCGTTCTCCTCTCCAACGACACGCGACGACACCCAATTGAATTGTGGTCGTAATTAGAAAAAGAAAAATATCCAAAACATTGCCCCATCTATAAAGAGACATCAAGATCCTCATACCACTCAAACCAACCACCAAGACAAAAAGATGAACAAACTCATAGCAATCTCCTCTTTCTTACTAGCCATCTCTCTTATCTCTCCCACTCTCAAGGCTGACATAGCCTTGGATTTGTCCACAGCTACCAAAGCATCCTATTCAGCTTTCATAACCCACATCAGGAATGCTCTTCCAACCAAAGCAACAGTGTGCAACATACCCCTGCTGCCTTCCACCCCAACGGGCTTAGCCTCGTTCACATTCTTCACCATCACCAATTATAACTACCAAACCGTCGAGGTCGCTGTGAACGTAACCAACGTCTACATCGTGGCGTATCGTGCCGGTGCAAGCTCCTACTTTTTCGAAGACACTCCAGCTCAAGCCAAACAACTCTTATTTGCAGGTACTCAAAAGTCCACTGCTGAAGCTGCGAGGTTTAAATATATTGAGCAACAGGTTGCTCAGCATATTAGCGATAAGTTTTATCCAAACCAAGCAGTGATAAGCTTAGAGAACAACTGGGGAGCTCTCTCCAAGCAAATCCAGATAGCAAATAGTACTGGAAGGGGACAATTCCAGAGCCCTGTTGAGCTTATAGGCCCTACTGGCACACGATTTAGTGTAACCAATACGTCCGCTGGGGTTGTTCAAGGCAATATCAAGCTCCTGCTGTACTACAAACTGAGCGTTGCTGATGACAACGACAGCCCGACAAGAATGCCTGACAATATTGTTGAATATGGAGCTCATGATTCTATGTAGCTCTATGGCTTTGTTTGAATATCCACACATAACAATGGATTGAGGCATGTTCATATGTGAATGTGCTTGATTTCATTAAATAATAAATAAGTGGGGATTTTGAAAAATTCGAGTTTTTTACTCCACATTGATTCATAGGGTTTGTGGCTCGAGTCTCTCGAAAAATAGATCAGTATAGTTACTATAGAACAAGTATATGAACGGAATTATACACTTGTACCCTAATGTCACATCCAAAAATGGTTTGATACCCAATT

Coding sequence (CDS)

ATGAACAAACTCATAGCAATCTCCTCTTTCTTACTAGCCATCTCTCTTATCTCTCCCACTCTCAAGGCTGACATAGCCTTGGATTTGTCCACAGCTACCAAAGCATCCTATTCAGCTTTCATAACCCACATCAGGAATGCTCTTCCAACCAAAGCAACAGTGTGCAACATACCCCTGCTGCCTTCCACCCCAACGGGCTTAGCCTCGTTCACATTCTTCACCATCACCAATTATAACTACCAAACCGTCGAGGTCGCTGTGAACGTAACCAACGTCTACATCGTGGCGTATCGTGCCGGTGCAAGCTCCTACTTTTTCGAAGACACTCCAGCTCAAGCCAAACAACTCTTATTTGCAGGTACTCAAAAGTCCACTGCTGAAGCTGCGAGGTTTAAATATATTGAGCAACAGGTTGCTCAGCATATTAGCGATAAGTTTTATCCAAACCAAGCAGTGATAAGCTTAGAGAACAACTGGGGAGCTCTCTCCAAGCAAATCCAGATAGCAAATAGTACTGGAAGGGGACAATTCCAGAGCCCTGTTGAGCTTATAGGCCCTACTGGCACACGATTTAGTGTAACCAATACGTCCGCTGGGGTTGTTCAAGGCAATATCAAGCTCCTGCTGTACTACAAACTGAGCGTTGCTGATGACAACGACAGCCCGACAAGAATGCCTGACAATATTGTTGAATATGGAGCTCATGATTCTATGTAG

Protein sequence

MNKLIAISSFLLAISLISPTLKADIALDLSTATKASYSAFITHIRNALPTKATVCNIPLLPSTPTGLASFTFFTITNYNYQTVEVAVNVTNVYIVAYRAGASSYFFEDTPAQAKQLLFAGTQKSTAEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGPTGTRFSVTNTSAGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM
Homology
[Close]
BLAST of Cp4.1LG17g10070 vs. Swiss-Prot
Match: RIP1_TRIAN (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina GN=TCA PE=1 SV=2)

HSP 1 Score: 160.2 bits (404), Expect = 2.7e-38
Identity = 118/275 (42.91%), Postives = 147/275 (53.45%), Query Frame = 1 

Query: 5   IAISSFLLAISLISPTLKADIALDLSTATKASYS-------------------------- 64
           +A+S F LAISL SPT   D++ DLSTATK SYS                          
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  --------AFITHIRNALPTKAT-VCNIPLLPSTPTGLASF----------TFFTITNY- 124
                     +T+  +   T A  V N+ ++      ++ F            F  T   
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 ------NYQTVEVAV----NVTNVYIVAYRAGASSYFFEDTPAQAKQLLFAGTQKSTAEA 184
                 NY  ++  V    ++  + I A  +  ++  + D  + A  LL     + TAEA
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVL--IQCTAEA 180

Query: 185 ARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGPTG 224
           AR+KYIEQQV+ HIS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL  P G
Sbjct: 181 ARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDG 240

[Close]
BLAST of Cp4.1LG17g10070 vs. Swiss-Prot
Match: RIP3_MOMCH (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia GN=MAP30 PE=1 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 2.9e-24
Identity = 65/145 (44.83%), Postives = 96/145 (66.21%), Query Frame = 1 

Query: 79  NYQTVEVAVNVT----NVYIVAYRAGASSYFFEDTPAQAKQLLFAGTQKSTAEAARFKYI 138
           NY+ ++ A +      ++ + A  +  ++ F+ +  +    LL     ++TAEAARFKYI
Sbjct: 131 NYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVL--IQTTAEAARFKYI 190

Query: 139 EQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGPTGTRFSVT 198
           E+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI PTG RF VT
Sbjct: 191 ERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQVT 250

Query: 199 NTSAGVVQGNIKLLLYYKLSVADDN 220
           N  + VV+GNIKLLL  + S AD+N
Sbjct: 251 NVDSDVVKGNIKLLLNSRASTADEN 272

[Close]
BLAST of Cp4.1LG17g10070 vs. Swiss-Prot
Match: RIP2_MOMBA (Ribosome-inactivating protein momordin II OS=Momordica balsamina PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.9e-24
Identity = 65/145 (44.83%), Postives = 96/145 (66.21%), Query Frame = 1 

Query: 79  NYQTVEVAVNVT----NVYIVAYRAGASSYFFEDTPAQAKQLLFAGTQKSTAEAARFKYI 138
           NY+ ++ A +      ++ + A  +  ++ F+ +  +    LL     ++TAEAARFKYI
Sbjct: 131 NYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVL--IQTTAEAARFKYI 190

Query: 139 EQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGPTGTRFSVT 198
           E+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI PTG RF VT
Sbjct: 191 ERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQVT 250

Query: 199 NTSAGVVQGNIKLLLYYKLSVADDN 220
           N  + VV+GNIKLLL  + S AD+N
Sbjct: 251 NVDSDVVKGNIKLLLNSRASTADEN 272

[Close]
BLAST of Cp4.1LG17g10070 vs. Swiss-Prot
Match: RIPT_TRIKI (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii PE=1 SV=2)

HSP 1 Score: 104.8 bits (260), Expect = 1.4e-21
Identity = 59/119 (49.58%), Postives = 77/119 (64.71%), Query Frame = 1 

Query: 104 YFFEDTPAQAKQLLFAGTQKSTAEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALS 163
           Y+  ++ A A  +L     +ST+EAAR+K+IEQQ+ + +   F P+ A+ISLEN+W ALS
Sbjct: 164 YYNANSAASALMVLI----QSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALS 223

Query: 164 KQIQIANSTGRGQFQSPVELIGPTGTRFSVTNTSAGVVQGNIKLLLYYKLSVADDNDSP 223
           KQIQIA ST  GQF+SPV LI     R ++TN  AGVV  NI LLL      A D+D P
Sbjct: 224 KQIQIA-STNNGQFESPVVLINAQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDDVP 277

[Close]
BLAST of Cp4.1LG17g10070 vs. Swiss-Prot
Match: RIPS_TRIKI (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii PE=1 SV=2)

HSP 1 Score: 103.6 bits (257), Expect = 3.0e-21
Identity = 58/119 (48.74%), Postives = 77/119 (64.71%), Query Frame = 1 

Query: 104 YFFEDTPAQAKQLLFAGTQKSTAEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALS 163
           Y+  ++ A A  +L     +ST+EAAR+K+IEQQ+ + +   F P+ A+ISLEN+W ALS
Sbjct: 164 YYNANSAASALMVLI----QSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALS 223

Query: 164 KQIQIANSTGRGQFQSPVELIGPTGTRFSVTNTSAGVVQGNIKLLLYYKLSVADDNDSP 223
           KQIQIA ST  GQF++PV LI     R ++TN  AGVV  NI LLL      A D+D P
Sbjct: 224 KQIQIA-STNNGQFETPVVLINAQNQRVTITNVDAGVVTSNIALLLNRNNMAAIDDDVP 277

[Close]
BLAST of Cp4.1LG17g10070 vs. TrEMBL
Match: S6CMM8_MOMCH (rRNA N-glycosidase OS=Momordica charantia GN=rip PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.6e-23
Identity = 68/146 (46.58%), Postives = 93/146 (63.70%), Query Frame = 1 

Query: 79  NYQTVEVAVNVTNVYIVAYRAGASS-----YFFEDTPAQAKQLLFAGTQKSTAEAARFKY 138
           NY+ ++ A +     I       SS     +++   PA +  L+   T   TAEAARFKY
Sbjct: 109 NYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQPAPSALLVLIQT---TAEAARFKY 168

Query: 139 IEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGPTGTRFSV 198
           IE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI PTG RF V
Sbjct: 169 IERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQV 228

Query: 199 TNTSAGVVQGNIKLLLYYKLSVADDN 220
           TN  + VV+GNIKLLL  + S AD+N
Sbjct: 229 TNVDSDVVKGNIKLLLNSRASTADEN 250

[Close]
BLAST of Cp4.1LG17g10070 vs. TrEMBL
Match: S6CM75_MOMCH (rRNA N-glycosidase OS=Momordica charantia GN=rip PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.6e-23
Identity = 65/145 (44.83%), Postives = 97/145 (66.90%), Query Frame = 1 

Query: 79  NYQTVEVAVNVT----NVYIVAYRAGASSYFFEDTPAQAKQLLFAGTQKSTAEAARFKYI 138
           NY+ ++ A +      ++ + A  +  ++ F+ +  +    LL     ++TAEAARFKYI
Sbjct: 109 NYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVL--IQTTAEAARFKYI 168

Query: 139 EQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGPTGTRFSVT 198
           E+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI PTG RF VT
Sbjct: 169 ERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQVT 228

Query: 199 NTSAGVVQGNIKLLLYYKLSVADDN 220
           N  + VV+GNIKLLL ++ S AD+N
Sbjct: 229 NVDSDVVKGNIKLLLNFRASTADEN 250

[Close]
BLAST of Cp4.1LG17g10070 vs. TrEMBL
Match: S6CNA6_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.6e-23
Identity = 68/146 (46.58%), Postives = 93/146 (63.70%), Query Frame = 1 

Query: 79  NYQTVEVAVNVTNVYIVAYRAGASS-----YFFEDTPAQAKQLLFAGTQKSTAEAARFKY 138
           NY+ ++ A +     I       SS     +++   PA +  L+   T   TAEAARFKY
Sbjct: 125 NYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQPAPSALLVLIQT---TAEAARFKY 184

Query: 139 IEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGPTGTRFSV 198
           IE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI PTG RF V
Sbjct: 185 IERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQV 244

Query: 199 TNTSAGVVQGNIKLLLYYKLSVADDN 220
           TN  + VV+GNIKLLL  + S AD+N
Sbjct: 245 TNVDSDVVKGNIKLLLNSRASTADEN 266

[Close]
BLAST of Cp4.1LG17g10070 vs. TrEMBL
Match: S6CNH7_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.1e-22
Identity = 58/97 (59.79%), Postives = 76/97 (78.35%), Query Frame = 1 

Query: 123 KSTAEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVE 182
           ++TAEAARFKYIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+
Sbjct: 171 QTTAEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVD 230

Query: 183 LIGPTGTRFSVTNTSAGVVQGNIKLLLYYKLSVADDN 220
           LI PTG RF VTN  + VV+GNIKLLL  + S AD+N
Sbjct: 231 LIKPTGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 266

[Close]
BLAST of Cp4.1LG17g10070 vs. TrEMBL
Match: S6CNB7_MOMCH (rRNA N-glycosidase OS=Momordica charantia GN=rip PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.1e-22
Identity = 58/97 (59.79%), Postives = 76/97 (78.35%), Query Frame = 1 

Query: 123 KSTAEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVE 182
           ++TAEAARFKYIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+
Sbjct: 155 QTTAEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVD 214

Query: 183 LIGPTGTRFSVTNTSAGVVQGNIKLLLYYKLSVADDN 220
           LI PTG RF VTN  + VV+GNIKLLL  + S AD+N
Sbjct: 215 LIKPTGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 250

[Close]
BLAST of Cp4.1LG17g10070 vs. NCBI nr
Match: gi|8247957|sp|P56626.2|RIP1_TRIAN (RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; AltName: Full=Trichoanguin; AltName: Full=rRNA N-glycosidase; Flags: Precursor)

HSP 1 Score: 160.2 bits (404), Expect = 4.4e-36
Identity = 118/275 (42.91%), Postives = 147/275 (53.45%), Query Frame = 1 

Query: 5   IAISSFLLAISLISPTLKADIALDLSTATKASYS-------------------------- 64
           +A+S F LAISL SPT   D++ DLSTATK SYS                          
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  --------AFITHIRNALPTKAT-VCNIPLLPSTPTGLASF----------TFFTITNY- 124
                     +T+  +   T A  V N+ ++      ++ F            F  T   
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 ------NYQTVEVAV----NVTNVYIVAYRAGASSYFFEDTPAQAKQLLFAGTQKSTAEA 184
                 NY  ++  V    ++  + I A  +  ++  + D  + A  LL     + TAEA
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVL--IQCTAEA 180

Query: 185 ARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGPTG 224
           AR+KYIEQQV+ HIS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL  P G
Sbjct: 181 ARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDG 240

[Close]
BLAST of Cp4.1LG17g10070 vs. NCBI nr
Match: gi|530776771|emb|CCD30683.1| (ribosome inactivating protein [Momordica charantia])

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-22
Identity = 68/146 (46.58%), Postives = 93/146 (63.70%), Query Frame = 1 

Query: 79  NYQTVEVAVNVTNVYIVAYRAGASS-----YFFEDTPAQAKQLLFAGTQKSTAEAARFKY 138
           NY+ ++ A +     I       SS     +++   PA +  L+   T   TAEAARFKY
Sbjct: 109 NYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQPAPSALLVLIQT---TAEAARFKY 168

Query: 139 IEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGPTGTRFSV 198
           IE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI PTG RF V
Sbjct: 169 IERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQV 228

Query: 199 TNTSAGVVQGNIKLLLYYKLSVADDN 220
           TN  + VV+GNIKLLL  + S AD+N
Sbjct: 229 TNVDSDVVKGNIKLLLNSRASTADEN 250

[Close]
BLAST of Cp4.1LG17g10070 vs. NCBI nr
Match: gi|530776765|emb|CCD30680.1| (ribosome inactivating protein [Momordica charantia])

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-22
Identity = 65/145 (44.83%), Postives = 97/145 (66.90%), Query Frame = 1 

Query: 79  NYQTVEVAVNVT----NVYIVAYRAGASSYFFEDTPAQAKQLLFAGTQKSTAEAARFKYI 138
           NY+ ++ A +      ++ + A  +  ++ F+ +  +    LL     ++TAEAARFKYI
Sbjct: 109 NYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVL--IQTTAEAARFKYI 168

Query: 139 EQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGPTGTRFSVT 198
           E+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI PTG RF VT
Sbjct: 169 ERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQVT 228

Query: 199 NTSAGVVQGNIKLLLYYKLSVADDN 220
           N  + VV+GNIKLLL ++ S AD+N
Sbjct: 229 NVDSDVVKGNIKLLLNFRASTADEN 250

[Close]
BLAST of Cp4.1LG17g10070 vs. NCBI nr
Match: gi|530776715|emb|CCD28543.1| (ribosome inactivating protein, partial [Momordica charantia])

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-22
Identity = 68/146 (46.58%), Postives = 93/146 (63.70%), Query Frame = 1 

Query: 79  NYQTVEVAVNVTNVYIVAYRAGASS-----YFFEDTPAQAKQLLFAGTQKSTAEAARFKY 138
           NY+ ++ A +     I       SS     +++   PA +  L+   T   TAEAARFKY
Sbjct: 125 NYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQPAPSALLVLIQT---TAEAARFKY 184

Query: 139 IEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGPTGTRFSV 198
           IE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI PTG RF V
Sbjct: 185 IERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQV 244

Query: 199 TNTSAGVVQGNIKLLLYYKLSVADDN 220
           TN  + VV+GNIKLLL  + S AD+N
Sbjct: 245 TNVDSDVVKGNIKLLLNSRASTADEN 266

[Close]
BLAST of Cp4.1LG17g10070 vs. NCBI nr
Match: gi|530776709|emb|CCD28540.1| (ribosome inactivating protein, partial [Momordica charantia])

HSP 1 Score: 115.2 bits (287), Expect = 1.6e-22
Identity = 58/97 (59.79%), Postives = 76/97 (78.35%), Query Frame = 1 

Query: 123 KSTAEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVE 182
           ++TAEAARFKYIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+
Sbjct: 171 QTTAEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVD 230

Query: 183 LIGPTGTRFSVTNTSAGVVQGNIKLLLYYKLSVADDN 220
           LI PTG RF VTN  + VV+GNIKLLL  + S AD+N
Sbjct: 231 LIKPTGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 266

The following BLAST results are available for this feature:
BLAST of Cp4.1LG17g10070 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RIP1_TRIAN2.7e-3842.91Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina GN=T... [more]
RIP3_MOMCH2.9e-2444.83Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia GN=MAP30 P... [more]
RIP2_MOMBA2.9e-2444.83Ribosome-inactivating protein momordin II OS=Momordica balsamina PE=3 SV=1[more]
RIPT_TRIKI1.4e-2149.58Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii PE=... [more]
RIPS_TRIKI3.0e-2148.74Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii PE=1 SV=2[more]
BLAST of Cp4.1LG17g10070 vs. TrEMBL
Match NameE-valueIdentityDescription
S6CMM8_MOMCH8.6e-2346.58rRNA N-glycosidase OS=Momordica charantia GN=rip PE=3 SV=1[more]
S6CM75_MOMCH8.6e-2344.83rRNA N-glycosidase OS=Momordica charantia GN=rip PE=3 SV=1[more]
S6CNA6_MOMCH8.6e-2346.58Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
S6CNH7_MOMCH1.1e-2259.79Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
S6CNB7_MOMCH1.1e-2259.79rRNA N-glycosidase OS=Momordica charantia GN=rip PE=3 SV=1[more]
BLAST of Cp4.1LG17g10070 vs. TAIR10
Match NameE-valueIdentityDescription
BLAST of Cp4.1LG17g10070 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|8247957|sp|P56626.2|RIP1_TRIAN4.4e-3642.91RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; Alt... [more]
gi|530776771|emb|CCD30683.1|1.2e-2246.58ribosome inactivating protein [Momordica charantia][more]
gi|530776765|emb|CCD30680.1|1.2e-2244.83ribosome inactivating protein [Momordica charantia][more]
gi|530776715|emb|CCD28543.1|1.2e-2246.58ribosome inactivating protein, partial [Momordica charantia][more]
gi|530776709|emb|CCD28540.1|1.6e-2259.79ribosome inactivating protein, partial [Momordica charantia][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0030598rRNA N-glycosylase activity
Vocabulary: Biological Process
TermDefinition
GO:0017148negative regulation of translation
Vocabulary: INTERPRO
TermDefinition
IPR017989Ribosome_inactivat_1/2
IPR016139Ribosome_inactivat_prot_sub2
IPR016138Ribosome_inactivat_prot_sub1
IPR001574Ribosome_inactivat_prot
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017148 negative regulation of translation
biological_process GO:0006952 defense response
cellular_component GO:0005575 cellular_component
molecular_function GO:0030598 rRNA N-glycosylase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG17g10070.1Cp4.1LG17g10070.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 125..167
score: 9.8E-6coord: 33..118
score: 9.5
IPR001574Ribosome-inactivating proteinunknownSSF56371Ribosome inactivating proteins (RIP)coord: 24..211
score: 1.96
IPR016138Ribosome-inactivating protein, subdomain 1GENE3DG3DSA:3.40.420.10coord: 24..126
score: 2.0
IPR016139Ribosome-inactivating protein, subdomain 2GENE3DG3DSA:4.10.470.10coord: 130..212
score: 1.9
IPR017989Ribosome-inactivating protein subgroupPRINTSPR00396SHIGARICINcoord: 35..48
score: 6.5E-7coord: 82..97
score: 6.

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG17g10070CmaCh08G001070Cucurbita maxima (Rimu)cmacpeB914
Cp4.1LG17g10070CmoCh08G001130Cucurbita moschata (Rifu)cmocpeB851
Cp4.1LG17g10070ClCG08G004120Watermelon (Charleston Gray)cpewcgB310
Cp4.1LG17g10070Bhi04G001651Wax gourdcpewgoB0409
Cp4.1LG17g10070Carg04936Silver-seed gourdcarcpeB0125
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG17g10070Cucurbita pepo (Zucchini)cpecpeB166
Cp4.1LG17g10070Cucurbita pepo (Zucchini)cpecpeB172
Cp4.1LG17g10070Cucurbita maxima (Rimu)cmacpeB390
Cp4.1LG17g10070Cucurbita maxima (Rimu)cmacpeB384
Cp4.1LG17g10070Cucurbita moschata (Rifu)cmocpeB349
Cp4.1LG17g10070Cucurbita moschata (Rifu)cmocpeB354
Cp4.1LG17g10070Wild cucumber (PI 183967)cpecpiB332
Cp4.1LG17g10070Cucumber (Chinese Long) v2cpecuB331
Cp4.1LG17g10070Bottle gourd (USVL1VR-Ls)cpelsiB251
Cp4.1LG17g10070Watermelon (Charleston Gray)cpewcgB301
Cp4.1LG17g10070Watermelon (Charleston Gray)cpewcgB311
Cp4.1LG17g10070Watermelon (97103) v1cpewmB337
Cp4.1LG17g10070Melon (DHL92) v3.5.1cpemeB287
Cp4.1LG17g10070Cucumber (Gy14) v2cgybcpeB359
Cp4.1LG17g10070Melon (DHL92) v3.6.1cpemedB373
Cp4.1LG17g10070Silver-seed gourdcarcpeB0312
Cp4.1LG17g10070Cucumber (Chinese Long) v3cpecucB0391
Cp4.1LG17g10070Wax gourdcpewgoB0412