CmoCh08G001130 (gene) Cucurbita moschata (Rifu)

NameCmoCh08G001130
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
Description(Ribosome inactivating protein) (3.2.2.22)
LocationCmo_Chr08 : 583200 .. 588238 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAAATCCTTAGCCCTTTCGTTTCTATTTCTCACCATCTTCCTCAACACTCGTCCCGCTGATGCCAATGTGCGTTTCGACTTGTCCAGTGCCACAAGCTCATCTTACAAAACTTTTATAAAAAATTTGAGGGAAGCACTTCCAAAGGATGGGAAAGTGTATGACATTCCTGTGTTACTTTCGACTGTAATGGACTCGAGGCGCTTCATACTAATAGATCTCGTCAATTACGACGGTCAATCCATCACAGCTGCCATAGATGTGCTAAACGTATATATCGTAGCATACAGCACAGGCACAGTGTCCTACTTTTTTCAGCAAGTTCCAGCTCAAGCTCCCAAATTGCTGTTCAAAGGCACTCAGCAAAGGACACTTCCATACACAGGTAATTACGAGAACCTTCAAACTGCTGCAAAAAAGCTAAGAGAAAACATCGAGCTTGGACTCCCAGCTCTAGACAGTGCCATTACCACCTTGTTTCATTACAACGCCGAGGCTGCTGCTTCAGCGCTGCTCGTTCTCATTCAAACGACCTCTGAAGCTGCAAGGTTTAGATATATCGAGCTACAGATTGCTAACAATGTTGGGACGAAATTTAAGCCAAGTCAAACGATCATAAGCTTGGAAAACAACTGGTCTGCGCTCTCCAAACAAATCCAAATTGCCAAGAACAAAAATGGGCAATTTGAAACTCCAGTTATCCTTATAGACCCTCAAGGAAATCGTGTTCAAATAACAAACGTGACTTCGAACGTTGTGACCCAGAACATCAAGCTGCTGCTGAACATTGGTGCAACAGTTGATACAATGGCCGCCAACGACGATGTGGATGAGATGACGAGCGGTGTTCCCATAAGGCTATCAAGCTGTGGCCATGAGATGGAAAGGAAGTCAGTGGAGAATGGCCGGCATGCAAATTATGTATCGGTGTAACTTTTGAAGTTACAGGTATAGTGGGTATTAAGACTTTAGTAGTCCACCCACAGAAATAAGGCATGTGTTCATTGTGTTTATGTGTTTTATTTCTTAAATGCTAAATAAAATGGAACGATGAAGTCCCTATGACCGATGAAGTCCCTATGACTCGACTAAACGGAATAAACGGGTCGTTGGGTATTATGGAGAAAGTTTATGATATAGATCTTGTGAGATCTCACCTCGGTTGGAGAGGGGAACAGAACATTCTTTATAAGGGTGTAGAAACCTCTCTCTAACAAACGTGTTTTAAAATTTTGAAGGGAAGCTCGAAAGGAAAAACTCAAAGAAGACAATATATGTTAGTTGTGAGCTCGATACATTGAGGCTGTCTCCTAGGAATTTAGATTTTTTTTTTTCTCTTTACATGATTGATATTTTTAGTTGGTTCTCGTCCCATTAGATTCCTCTTTGAAGAGATCTATAATTTTGAAGAATTCAGAGTTGTTGAGATAATAATCGAAGTATTTGAACATGAAAATAATCGAATAGTGAAATCGAAGATGTGTGCATAAAAGATGACCAATTTAGTTGGACCAATCCAATTCAATTTTTGGGTTATTTTGACGATGTTGATATTTTCTCCTATTTTCGAGACTACATACGAGACTCAAAGAAAGTTGGAGTGGAAAAACAGCAAGTTTTGTCTTCGTCCCGTAGACATCTCTTACCACCTATATTTATTGAAGTAAAAAAAAAAAAAAAAAAAAGTTGTAAATCGAGATAAGATATTGATTGGTGGGAGAGTGAACGAAGAAAATATCTATATTAAACTCTTTTTATGCTTATTACCATCATTTTTATGTGTTTGATACACAAAAACCTATTATTGATATACTATTCCTACGCCTATCAATGATAGATATCTATTAGTGATACATATAGGCGTAACAAAAATTATATTACGTGTATATTTGTATTCAACAAGGGATATTACGCCCATTGATCATTGCATTGTAATTTGAGCCGAAGCTAGTGAGGTTCTAGGCGCCTTTGGGCGATATTGTGGCTTTCTAGAAGATTCACATTTGAGTCTCAAGATCTTTTTTTTTTTTTTTGGAGATTTGTGATAGTTTATTATTCCTCGAGTGTTTGTTATTGTTATTCCTTTATTTGGTTCTTGATAATCCCTTTGCTAGAACTCTTTTCCTGCCTGAATTATCGCATCTTGGGATGACTTTGGAGAGAGTTTCTTAATCAAGTATTTTCTACGAATGATGTAAAGGATATCATGACATTTAAGTTATGAGAGAATGAATCACTCAGGGATCCGTATGTGAGGTTTAGAAAGTTGTAGCAAAAAATGTGGTTTGCCCTATTGTGTACCGTTAGAAATGTTCACAAAAGTTTGAACATAGTGATATGAAACTCTAGGACCTAAGAACCTTTGTGGATATATAAACCCTAAGACTACAAATGCTAAAACTCCCAAATCAAGATGATTTCATCCCAATGATATCAAACTTCGAGAATTCAATCTAACAACCTTATTAAAAAACAAGATTTTTAAATCTAAAATATCTAAGAACCCCAAAACAATAAGAAATCAACCCAAAGAAAAGATTGAGATCGAGATTAGAATTACTCATGCCTCAGTTTATATCACATAGTTTCACCAACTTATAACATAGTCTCACCAACTCCAAGAGGTATGTGTTGTCTTTAAGCATTTGGAGCTTTGATGGAAAAGTGAATCTAAAAACATAGTAAAATTTCTTATTTGGATTAGTGATAAGACAATTGTTGTAATTACAGACAAAATTTACCGATTGCAACCGAGCTTTGTGGCACTATTCGAGGAAGCAATGATTCAAGTTTGGGGTTTGATACCTTGTAAATGTGCAAGTTATTTGGGTAGTATTCACTTTGAGAATGAATTCTATGCGTTTATCCAAATTTTATTGTGAAATCATGTCTAATTCATCCGTTTGCATCAATAGTTAAATTATAAGATTGTAGTTTTATAAGCTCTGAAGGACTAACATAAGAGAGAAAACGTGCTTCAACATGTGAACCCTATCTCTCCATTCATATATCAATGTCATGTGTTATCAAAATTGGTTGTACCAATAATTCCAAGAGATGCGGAAAGTTGTGGAGGGCTATAAACCATACAATTGGTATCCCATGGTACGTTGTTTTCTTCATAGATTTTTTTTTTTTTGGTTAAATTATAATTTTAGTTTTTGAACGTCTATGATTTTTTAGTTTGAATGTATAGAGATTTTGTTAAGTTTATATTTATTAAGTTTGTGTTTAGACGGTACAAAAAGTAGCTATGTTAAATCTAAAGATCGAACTATCTTGAATTCTCCATCTACCAGCTACAGGTTGGGATTCGATTCCCGTTATACATGACGAGTATCTTCAAAACAAAATTGTTAGTACTTTATGGGAAACTTCTCAGCAAGTTCCTCAATATCGTTCGTCCTTAGGGCCATTATGCTCCTTCAGGTATTCTCGTATCCTTTCGGCTACGGTGCTTGAAAATGTACGTACACGAATGGTTCCTAGACATGGACCACACAGGAAAAAGGGATTATACTATAGTACTCTTAGATGGCTCCTTAAAAACCCCAGCTTGTGATGCTCCAAAGGCATCCAAAGTCCTCTAGTGGAAAACATCCTAAGAGGAGCTCAAACAGTCAAAGGCGATGTCAGCTTCCGTTTATCGGGTGCAACAACCAGTTCCTATGGAGTTTTCATTTCAAATCTGAGAAAAGCTCTTCCATATGAAAAGAAATTGTACGATATTCCTCTGTTACGTTCCGATCTTTCAGGTTCACAGCGCTACACATTAGTCCATCTCACAAATTACGCCGATCAAACCATTTCGGTTGCCATAGACGTAACAAACGTGTATATTATGGGGTATCGTGCTGGTAACGTTTCCTACTTTTCCTACTTTTTCGGATTGGATTGGGTAGAGTAGTCGGGTTGTTTTTCTTTTTTAAAAAAAAAATTCTATTTAACCTGAATATAGGTTATATGATGTAAATTACATTGATAACTAAAAATTCATAAAGCTCAAATTTCAAATAATTTTAAAAGTAGAGTAGGGTAGTGTCAGACTGTAACCCTATTTTTTAGTTTCTCAGGTTGACCTGACTAAAAAAATGTTAACCTACAAAGAGGCTCCAATTTTCTGCTTTCTAAAACGTTCAACCCAACCCAAACTATAAAATAAAAATAATTTAATCCAACCCAACATTTCTAATTTCAGATGAGTTGCATGAGTTGATCGAGTTATTAGATTATATAAACACGTCAAAGTTAGATGAACTTGACTCTGAGAATGTTTCTTAATTCACCCCACCTAAATGGTGTGAAAGTTATCTTATTTATAATCAAATAAATTACAAGGATATGGAAATAGTAATAAATGGAAAGATATTTGTCTTATAATAAAATATCAAATATGATATACATGGTAAACTATAATTTATTACTAAATATCCTTAACATACTCATTGAAGAAGATCAGAGGATTTAACTCATTTTCAAAAGGGAAGGCGGTTGAATACACTTTGAATCAAACCCACGCTCGTCGGTAAACTTCCTACATAGATTTGCGAGCAAATAGTGAATGATCGAGCTCGATACATTGAGGCTATTGTTGTGATCGAAGCCTCCTAGGAATCCAGATTTTTTTTTTTTCCGTTTACATGATTGATATTTTTAGTTGATTCTCGTCCCATTAGATTCCTCTTGAAGAGATCTATAATTTTGAAGAATCCAGAGTTGTTGAGATAATAATCGAAGTGTTTGAACATGAAAATAATCGAATAGTGAAATCGAAGATGTGTGCATAAAAGATGACCAATTTAGTTGGACCAATCCAATTCAATTCCTGAGTTATTTTGACGATGTTGATATTTTCTCCTATTTTCGAGACTACATACGAGACTCAAAGAAAGTTGGAGTGGAAAAACAGCAAGTTTTGTCTTCGCCATGTAGACATCTCTTACCACCTATATTTATTGAAGTAAAAAAAAAAAAAAAAAGTTATAAATCGAGATAAGATATTGATTGGTGGGAGAGTGAACGAAGAAAATATCTAG

mRNA sequence

ATGAACAAATCCTTAGCCCTTTCGTTTCTATTTCTCACCATCTTCCTCAACACTCGTCCCGCTGATGCCAATGTGCGTTTCGACTTGTCCAGTGCCACAAGCTCATCTTACAAAACTTTTATAAAAAATTTGAGGGAAGCACTTCCAAAGGATGGGAAAGTGTATGACATTCCTGTGTTACTTTCGACTGTAATGGACTCGAGGCGCTTCATACTAATAGATCTCGTCAATTACGACGGTCAATCCATCACAGCTGCCATAGATGTGCTAAACGTATATATCGTAGCATACAGCACAGGCACAGTGTCCTACTTTTTTCAGCAAGTTCCAGCTCAAGCTCCCAAATTGCTGTTCAAAGGCACTCAGCAAAGGACACTTCCATACACAGGTAATTACGAGAACCTTCAAACTGCTGCAAAAAAGCTAAGAGAAAACATCGAGCTTGGACTCCCAGCTCTAGACAGTGCCATTACCACCTTGTTTCATTACAACGCCGAGGCTGCTGCTTCAGCGCTGCTCGTTCTCATTCAAACGACCTCTGAAGCTGCAAGGTTTAGATATATCGAGCTACAGATTGCTAACAATGTTGGGACGAAATTTAAGCCAAGTCAAACGATCATAAGCTTGGAAAACAACTGGTCTGCGCTCTCCAAACAAATCCAAATTGCCAAGAACAAAAATGGGCAATTTGAAACTCCAGTTATCCTTATAGACCCTCAAGGAAATCGTGTTCAAATAACAAACGTGACTTCGAACGTTGTGACCCAGAACATCAAGCTGCTGCTGAACATTGGTGCAACAGTTGATACAATGGCCGCCAACGACGATGTGGATGAGATGACGAGCGGTGTTCCCATAAGGCTATCAAGCTGTGGCCATGAGATGGAAAGGAAGTCAGTGGAGAATGGCCGGCATGCAAATTATGTATCGGTACAAAATTTACCGATTGCAACCGAGCTTTGTGGCACTATTCGAGGAAGCAATGATTCAAGTTGGGATTCGATTCCCGTTATACATGACGAGTATCTTCAAAACAAAATTGTTAGTACTTTATGGGAAACTTCTCAGCAAGTTCCTCAATATCGTTCGTCCTTAGGGCCATTATGCTCCTTCAGGTATTCTCGTATCCTTTCGGCTACGGTGCTTGAAAATGTACGTACACGAATGGTTCCTAGACATGGACCACACAGGAAAAAGGGATTATACTATAGCATCCAAAGTCCTCTAGTGGAAAACATCCTAAGAGGAGCTCAAACAGTCAAAGGCGATGTCAGCTTCCGTTTATCGGGTGCAACAACCAGTTCCTATGGAGTTTTCATTTCAAATCTGAGAAAAGCTCTTCCATATGAAAAGAAATTGTACGATATTCCTCTGTTACGTTCCGATCTTTCAGGTTCACAGCGCTACACATTAGTCCATCTCACAAATTACGCCGATCAAACCATTTCGGTTGCCATAGACATTCCTCTTGAAGAGATCTATAATTTTGAAGAATCCAGAGTTGTTGAGATAATAATCGAAGTGTTTGAACATGAAAATAATCGAATAATATTGATTGGTGGGAGAGTGAACGAAGAAAATATCTAG

Coding sequence (CDS)

ATGAACAAATCCTTAGCCCTTTCGTTTCTATTTCTCACCATCTTCCTCAACACTCGTCCCGCTGATGCCAATGTGCGTTTCGACTTGTCCAGTGCCACAAGCTCATCTTACAAAACTTTTATAAAAAATTTGAGGGAAGCACTTCCAAAGGATGGGAAAGTGTATGACATTCCTGTGTTACTTTCGACTGTAATGGACTCGAGGCGCTTCATACTAATAGATCTCGTCAATTACGACGGTCAATCCATCACAGCTGCCATAGATGTGCTAAACGTATATATCGTAGCATACAGCACAGGCACAGTGTCCTACTTTTTTCAGCAAGTTCCAGCTCAAGCTCCCAAATTGCTGTTCAAAGGCACTCAGCAAAGGACACTTCCATACACAGGTAATTACGAGAACCTTCAAACTGCTGCAAAAAAGCTAAGAGAAAACATCGAGCTTGGACTCCCAGCTCTAGACAGTGCCATTACCACCTTGTTTCATTACAACGCCGAGGCTGCTGCTTCAGCGCTGCTCGTTCTCATTCAAACGACCTCTGAAGCTGCAAGGTTTAGATATATCGAGCTACAGATTGCTAACAATGTTGGGACGAAATTTAAGCCAAGTCAAACGATCATAAGCTTGGAAAACAACTGGTCTGCGCTCTCCAAACAAATCCAAATTGCCAAGAACAAAAATGGGCAATTTGAAACTCCAGTTATCCTTATAGACCCTCAAGGAAATCGTGTTCAAATAACAAACGTGACTTCGAACGTTGTGACCCAGAACATCAAGCTGCTGCTGAACATTGGTGCAACAGTTGATACAATGGCCGCCAACGACGATGTGGATGAGATGACGAGCGGTGTTCCCATAAGGCTATCAAGCTGTGGCCATGAGATGGAAAGGAAGTCAGTGGAGAATGGCCGGCATGCAAATTATGTATCGGTACAAAATTTACCGATTGCAACCGAGCTTTGTGGCACTATTCGAGGAAGCAATGATTCAAGTTGGGATTCGATTCCCGTTATACATGACGAGTATCTTCAAAACAAAATTGTTAGTACTTTATGGGAAACTTCTCAGCAAGTTCCTCAATATCGTTCGTCCTTAGGGCCATTATGCTCCTTCAGGTATTCTCGTATCCTTTCGGCTACGGTGCTTGAAAATGTACGTACACGAATGGTTCCTAGACATGGACCACACAGGAAAAAGGGATTATACTATAGCATCCAAAGTCCTCTAGTGGAAAACATCCTAAGAGGAGCTCAAACAGTCAAAGGCGATGTCAGCTTCCGTTTATCGGGTGCAACAACCAGTTCCTATGGAGTTTTCATTTCAAATCTGAGAAAAGCTCTTCCATATGAAAAGAAATTGTACGATATTCCTCTGTTACGTTCCGATCTTTCAGGTTCACAGCGCTACACATTAGTCCATCTCACAAATTACGCCGATCAAACCATTTCGGTTGCCATAGACATTCCTCTTGAAGAGATCTATAATTTTGAAGAATCCAGAGTTGTTGAGATAATAATCGAAGTGTTTGAACATGAAAATAATCGAATAATATTGATTGGTGGGAGAGTGAACGAAGAAAATATCTAG
BLAST of CmoCh08G001130 vs. Swiss-Prot
Match: RIP_CUCMO (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata PE=1 SV=2)

HSP 1 Score: 461.8 bits (1187), Expect = 9.8e-129
Identity = 243/244 (99.59%), Postives = 244/244 (100.00%), Query Frame = 1

Query: 24  NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 83
           NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 60

Query: 84  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 143
           TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR
Sbjct: 61  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 120

Query: 144 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 203
           ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS
Sbjct: 121 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 180

Query: 204 QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIKLLLN 263
           QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNI+LLLN
Sbjct: 181 QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQLLLN 240

Query: 264 IGAT 268
           IGAT
Sbjct: 241 IGAT 244

BLAST of CmoCh08G001130 vs. Swiss-Prot
Match: RIP2_MOMBA (Ribosome-inactivating protein momordin II OS=Momordica balsamina PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 3.3e-84
Identity = 167/267 (62.55%), Postives = 205/267 (76.78%), Query Frame = 1

Query: 1   MNKSLALSFLFLTIFLNTRPADANVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVL 60
           M K L LSFL + IF+    A  +V FDLS+AT+ +Y  FI++ R  LP   KVYDIP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  LSTVMDSRRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKG 120
            ST+ DSRRFIL+DL +Y  ++I+ AIDV NVY+VAY T  VSYFF++ P +A  +LFKG
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQQRTLPYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTS 180
           T++ TLPYTGNYENLQTAA K+RENI+LGLPAL SAITTLF+YNA++A SALLVLIQTT+
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFRYIELQIANNVGTKFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQ 240
           EAARF+YIE  +A  V T FKP+  IISLEN WSALSKQI +A+N+ G+F  PV LI P 
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPT 240

Query: 241 GNRVQITNVTSNVVTQNIKLLLNIGAT 268
           G R Q+TNV S+VV  NIKLLLN  A+
Sbjct: 241 GERFQVTNVDSDVVKGNIKLLLNSRAS 267

BLAST of CmoCh08G001130 vs. Swiss-Prot
Match: RIP3_MOMCH (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia GN=MAP30 PE=1 SV=2)

HSP 1 Score: 312.0 bits (798), Expect = 1.2e-83
Identity = 166/267 (62.17%), Postives = 205/267 (76.78%), Query Frame = 1

Query: 1   MNKSLALSFLFLTIFLNTRPADANVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVL 60
           M K L LSFL + IF+    A  +V FDLS+AT+ +Y  FI++ R  LP   KVYDIP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  LSTVMDSRRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKG 120
            ST+ DSRRFIL++L +Y  ++I+ AIDV NVY+VAY T  VSYFF++ P +A  +LFKG
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQQRTLPYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTS 180
           T++ TLPYTGNYENLQTAA K+RENI+LGLPAL SAITTLF+YNA++A SALLVLIQTT+
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFRYIELQIANNVGTKFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQ 240
           EAARF+YIE  +A  V T FKP+  IISLEN WSALSKQI +A+N+ G+F  PV LI P 
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPT 240

Query: 241 GNRVQITNVTSNVVTQNIKLLLNIGAT 268
           G R Q+TNV S+VV  NIKLLLN  A+
Sbjct: 241 GERFQVTNVDSDVVKGNIKLLLNSRAS 267

BLAST of CmoCh08G001130 vs. Swiss-Prot
Match: RIPT_TRIKI (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii PE=1 SV=2)

HSP 1 Score: 296.2 bits (757), Expect = 7.1e-79
Identity = 169/283 (59.72%), Postives = 205/283 (72.44%), Query Frame = 1

Query: 1   MNKSLALSFLFLTIFLNTRPADANVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVL 60
           M + L LS L LT+FL T   + +V F LS ATSSSY  FI NLR+ALP + K+YDIP+L
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  LSTVMDSRRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQVPA-QAPKLLFK 120
            S++  S+R+ LI L NY  ++I+ AIDV NVYI+ Y  G  SYFF +  A +A K +FK
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 GTQQR-TLPYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHYNAEAAASALLVLIQT 180
              ++ TLPY+GNYE LQTAA K+RENI LGLPALDSAITTLF+YNA +AASAL+VLIQ+
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TSEAARFRYIELQIANNVGTKFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILID 240
           TSEAAR+++IE QI   V   F PS  IISLEN+WSALSKQIQIA   NGQFE+PV+LI+
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN 240

Query: 241 PQGNRVQITNVTSNVVTQNIKLLLNIGATVDTMAANDDVDEMT 282
            Q  RV ITNV + VVT NI LLLN     + MAA DD   MT
Sbjct: 241 AQNQRVTITNVDAGVVTSNIALLLN----RNNMAAMDDDVPMT 279

BLAST of CmoCh08G001130 vs. Swiss-Prot
Match: RIPS_TRIKI (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii PE=1 SV=2)

HSP 1 Score: 291.2 bits (744), Expect = 2.3e-77
Identity = 165/278 (59.35%), Postives = 201/278 (72.30%), Query Frame = 1

Query: 1   MNKSLALSFLFLTIFLNTRPADANVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVL 60
           M + L  S L LT+FL     + +V F LS ATSSSY  FI NLR+ALP + K+YDIP+L
Sbjct: 1   MIRFLVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYERKLYDIPLL 60

Query: 61  LSTVMDSRRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQVPA-QAPKLLFK 120
            ST+  S+R+ LI L NY  ++I+ AIDV NVY++ Y  G  SYFF +  A +A K +FK
Sbjct: 61  RSTLPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 GTQQR-TLPYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHYNAEAAASALLVLIQT 180
             +++ TLPY+GNYE LQ AA K+RENI LGLPALDSAITTLF+YNA +AASAL+VLIQ+
Sbjct: 121 DAKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TSEAARFRYIELQIANNVGTKFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILID 240
           TSEAAR+++IE QI   V   F PS  IISLEN+WSALSKQIQIA   NGQFETPV+LI+
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLIN 240

Query: 241 PQGNRVQITNVTSNVVTQNIKLLLNIGATVDTMAANDD 277
            Q  RV ITNV + VVT NI LLLN     + MAA DD
Sbjct: 241 AQNQRVTITNVDAGVVTSNIALLLN----RNNMAAIDD 274

BLAST of CmoCh08G001130 vs. TrEMBL
Match: D0VWS7_CUCMO (rRNA N-glycosidase OS=Cucurbita moschata PE=1 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.1e-126
Identity = 243/244 (99.59%), Postives = 244/244 (100.00%), Query Frame = 1

Query: 24  NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 83
           NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 60

Query: 84  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 143
           TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR
Sbjct: 61  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 120

Query: 144 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 203
           ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS
Sbjct: 121 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 180

Query: 204 QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIKLLLN 263
           QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNI+LLLN
Sbjct: 181 QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQLLLN 240

Query: 264 IGAT 268
           IGAT
Sbjct: 241 IGAT 244

BLAST of CmoCh08G001130 vs. TrEMBL
Match: W0LQW1_MOMCH (rRNA N-glycosidase OS=Momordica charantia PE=2 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 2.8e-82
Identity = 167/267 (62.55%), Postives = 205/267 (76.78%), Query Frame = 1

Query: 1   MNKSLALSFLFLTIFLNTRPADANVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVL 60
           M K L LSFL + IF+    A  +V FDLS+AT+ +Y  FI+N R  LP   KVYDIP+L
Sbjct: 1   MVKCLVLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIENFRATLPFSHKVYDIPLL 60

Query: 61  LSTVMDSRRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKG 120
            ST+ DSRRFIL++L +Y  ++I+ AIDV NVY+VAY T  VSYFF++ P +A  +LFKG
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQQRTLPYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTS 180
           T++ TLPYTGNYENLQTAA K+RENI+LGLPAL SAITTLF+YNA++A SALLVLIQTT+
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFRYIELQIANNVGTKFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQ 240
           EAARF+YIE  +A  V T FKP+  IISLEN WSALSKQI +A+N+ G+F  PV LI P 
Sbjct: 181 EAARFKYIERHVARYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPT 240

Query: 241 GNRVQITNVTSNVVTQNIKLLLNIGAT 268
           G R Q+TNV S+VV  NIKLLLN  A+
Sbjct: 241 GERFQVTNVDSDVVKGNIKLLLNSRAS 267

BLAST of CmoCh08G001130 vs. TrEMBL
Match: Q5PZ05_MOMCH (rRNA N-glycosidase OS=Momordica charantia PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.4e-81
Identity = 166/267 (62.17%), Postives = 205/267 (76.78%), Query Frame = 1

Query: 1   MNKSLALSFLFLTIFLNTRPADANVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVL 60
           M K L LSFL + IF+    A  +V FDLS+AT+ +Y  FI++ R  LP   KVYDIP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  LSTVMDSRRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKG 120
            ST+ DSRRFIL++L +Y  ++I+ AIDV NVY+VAY T  VSYFF++ P +A  +LFKG
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQQRTLPYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTS 180
           T++ TLPYTGNYENLQTAA K+RENI+LGLPAL SAITTLF+YNA++A SALLVLIQTT+
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFRYIELQIANNVGTKFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQ 240
           EAARF+YIE  +A  V T FKP+  IISLEN WSALSKQI +A+N+ G+F  PV LI P 
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPT 240

Query: 241 GNRVQITNVTSNVVTQNIKLLLNIGAT 268
           G R Q+TNV S+VV  NIKLLLN  A+
Sbjct: 241 GERFQVTNVDSDVVKGNIKLLLNSRAS 267

BLAST of CmoCh08G001130 vs. TrEMBL
Match: S6CNY4_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.8e-81
Identity = 164/261 (62.84%), Postives = 203/261 (77.78%), Query Frame = 1

Query: 7   LSFLFLTIFLNTRPADANVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMD 66
           LSFL + IF+    A  +V FDLS+AT+ +Y  FI++ R  LP   KVYDIP+L ST+ D
Sbjct: 1   LSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISD 60

Query: 67  SRRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTL 126
           SRRFIL+DL +Y  ++I+ AIDV NVY+VAY T  VSYFF++ P +A  +LFKGT++ TL
Sbjct: 61  SRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITL 120

Query: 127 PYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFR 186
           PYTGNYENLQTAA K+RENI+LGLPAL SAITTLF+YNA++A SALLVLIQTT+EAARF+
Sbjct: 121 PYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTAEAARFK 180

Query: 187 YIELQIANNVGTKFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQI 246
           YIE  +A  V T FKP+  IISLEN WSALSKQI +A+N+ G+F  PV LI+P G R Q+
Sbjct: 181 YIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIEPTGERFQV 240

Query: 247 TNVTSNVVTQNIKLLLNIGAT 268
           TNV S+VV  NIKLLLN  A+
Sbjct: 241 TNVDSDVVKGNIKLLLNSRAS 261

BLAST of CmoCh08G001130 vs. TrEMBL
Match: S6CNA9_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 4.0e-81
Identity = 164/261 (62.84%), Postives = 202/261 (77.39%), Query Frame = 1

Query: 7   LSFLFLTIFLNTRPADANVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMD 66
           LSFL + IF+    A  +V FDLS+AT+ +Y  FI++ R  LP   KVYDIP+L ST+ D
Sbjct: 1   LSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISD 60

Query: 67  SRRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTL 126
           SRRFIL+DL +Y  ++I+ AIDV NVY+VAY T  VSYFF++ P +A  +LFKGT++ TL
Sbjct: 61  SRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITL 120

Query: 127 PYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFR 186
           PYTGNYENLQTAA K+RENI+LGLPAL SAITTLF+YNA++A SALLVLIQTT+EAARF+
Sbjct: 121 PYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTAEAARFK 180

Query: 187 YIELQIANNVGTKFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQI 246
           YIE  +A  V T FKP+  IISLEN WSALSKQI +A+N+ G+F  PV LI P G R Q+
Sbjct: 181 YIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQV 240

Query: 247 TNVTSNVVTQNIKLLLNIGAT 268
           TNV S+VV  NIKLLLN  A+
Sbjct: 241 TNVDSDVVKGNIKLLLNSRAS 261

BLAST of CmoCh08G001130 vs. NCBI nr
Match: gi|281185483|sp|P84531.2|RIP_CUCMO (RecName: Full=Ribosome-inactivating protein cucurmosin; AltName: Full=rRNA N-glycosidase [Cucurbita moschata])

HSP 1 Score: 461.8 bits (1187), Expect = 1.6e-126
Identity = 243/244 (99.59%), Postives = 244/244 (100.00%), Query Frame = 1

Query: 24  NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 83
           NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 60

Query: 84  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 143
           TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR
Sbjct: 61  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 120

Query: 144 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 203
           ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS
Sbjct: 121 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 180

Query: 204 QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIKLLLN 263
           QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNI+LLLN
Sbjct: 181 QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQLLLN 240

Query: 264 IGAT 268
           IGAT
Sbjct: 241 IGAT 244

BLAST of CmoCh08G001130 vs. NCBI nr
Match: gi|209156420|pdb|3BWH|A (Chain A, Atomic Resolution Structure Of Cucurmosin, A Novel Type 1 Rip From The Sarcocarp Of Cucurbita Moschata)

HSP 1 Score: 461.8 bits (1187), Expect = 1.6e-126
Identity = 243/244 (99.59%), Postives = 244/244 (100.00%), Query Frame = 1

Query: 24  NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 83
           NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 60

Query: 84  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 143
           TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR
Sbjct: 61  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 120

Query: 144 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 203
           ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS
Sbjct: 121 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 180

Query: 204 QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIKLLLN 263
           QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNI+LLLN
Sbjct: 181 QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQLLLN 240

Query: 264 IGAT 268
           IGAT
Sbjct: 241 IGAT 244

BLAST of CmoCh08G001130 vs. NCBI nr
Match: gi|163867055|gb|ABY47624.1| (ribosome-inactivating protein [Cucurbita moschata])

HSP 1 Score: 448.4 bits (1152), Expect = 1.8e-122
Identity = 235/236 (99.58%), Postives = 236/236 (100.00%), Query Frame = 1

Query: 24  NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 83
           NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 60

Query: 84  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 143
           TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR
Sbjct: 61  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 120

Query: 144 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 203
           ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS
Sbjct: 121 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 180

Query: 204 QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIK 260
           QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNI+
Sbjct: 181 QTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQ 236

BLAST of CmoCh08G001130 vs. NCBI nr
Match: gi|574452326|gb|AHG25320.1| (ribosome-inactivating protein [Momordica charantia])

HSP 1 Score: 314.3 bits (804), Expect = 4.0e-82
Identity = 167/267 (62.55%), Postives = 205/267 (76.78%), Query Frame = 1

Query: 1   MNKSLALSFLFLTIFLNTRPADANVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVL 60
           M K L LSFL + IF+    A  +V FDLS+AT+ +Y  FI+N R  LP   KVYDIP+L
Sbjct: 1   MVKCLVLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIENFRATLPFSHKVYDIPLL 60

Query: 61  LSTVMDSRRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKG 120
            ST+ DSRRFIL++L +Y  ++I+ AIDV NVY+VAY T  VSYFF++ P +A  +LFKG
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQQRTLPYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTS 180
           T++ TLPYTGNYENLQTAA K+RENI+LGLPAL SAITTLF+YNA++A SALLVLIQTT+
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFRYIELQIANNVGTKFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQ 240
           EAARF+YIE  +A  V T FKP+  IISLEN WSALSKQI +A+N+ G+F  PV LI P 
Sbjct: 181 EAARFKYIERHVARYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPT 240

Query: 241 GNRVQITNVTSNVVTQNIKLLLNIGAT 268
           G R Q+TNV S+VV  NIKLLLN  A+
Sbjct: 241 GERFQVTNVDSDVVKGNIKLLLNSRAS 267

BLAST of CmoCh08G001130 vs. NCBI nr
Match: gi|132581|sp|P29339.1|RIP2_MOMBA (RecName: Full=Ribosome-inactivating protein momordin II; AltName: Full=rRNA N-glycosidase; Flags: Precursor)

HSP 1 Score: 313.9 bits (803), Expect = 5.3e-82
Identity = 167/267 (62.55%), Postives = 205/267 (76.78%), Query Frame = 1

Query: 1   MNKSLALSFLFLTIFLNTRPADANVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVL 60
           M K L LSFL + IF+    A  +V FDLS+AT+ +Y  FI++ R  LP   KVYDIP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  LSTVMDSRRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKG 120
            ST+ DSRRFIL+DL +Y  ++I+ AIDV NVY+VAY T  VSYFF++ P +A  +LFKG
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQQRTLPYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTS 180
           T++ TLPYTGNYENLQTAA K+RENI+LGLPAL SAITTLF+YNA++A SALLVLIQTT+
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFRYIELQIANNVGTKFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQ 240
           EAARF+YIE  +A  V T FKP+  IISLEN WSALSKQI +A+N+ G+F  PV LI P 
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPT 240

Query: 241 GNRVQITNVTSNVVTQNIKLLLNIGAT 268
           G R Q+TNV S+VV  NIKLLLN  A+
Sbjct: 241 GERFQVTNVDSDVVKGNIKLLLNSRAS 267

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RIP_CUCMO9.8e-12999.59Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata PE=1 SV=2[more]
RIP2_MOMBA3.3e-8462.55Ribosome-inactivating protein momordin II OS=Momordica balsamina PE=3 SV=1[more]
RIP3_MOMCH1.2e-8362.17Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia GN=MAP30 P... [more]
RIPT_TRIKI7.1e-7959.72Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii PE=... [more]
RIPS_TRIKI2.3e-7759.35Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii PE=1 SV=2[more]
Match NameE-valueIdentityDescription
D0VWS7_CUCMO1.1e-12699.59rRNA N-glycosidase OS=Cucurbita moschata PE=1 SV=1[more]
W0LQW1_MOMCH2.8e-8262.55rRNA N-glycosidase OS=Momordica charantia PE=2 SV=1[more]
Q5PZ05_MOMCH1.4e-8162.17rRNA N-glycosidase OS=Momordica charantia PE=3 SV=1[more]
S6CNY4_MOMCH1.8e-8162.84Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
S6CNA9_MOMCH4.0e-8162.84Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
gi|281185483|sp|P84531.2|RIP_CUCMO1.6e-12699.59RecName: Full=Ribosome-inactivating protein cucurmosin; AltName: Full=rRNA N-gly... [more]
gi|209156420|pdb|3BWH|A1.6e-12699.59Chain A, Atomic Resolution Structure Of Cucurmosin, A Novel Type 1 Rip From The ... [more]
gi|163867055|gb|ABY47624.1|1.8e-12299.58ribosome-inactivating protein [Cucurbita moschata][more]
gi|574452326|gb|AHG25320.1|4.0e-8262.55ribosome-inactivating protein [Momordica charantia][more]
gi|132581|sp|P29339.1|RIP2_MOMBA5.3e-8262.55RecName: Full=Ribosome-inactivating protein momordin II; AltName: Full=rRNA N-gl... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001574Ribosome_inactivat_prot
IPR016138Ribosome_inactivat_prot_sub1
IPR016139Ribosome_inactivat_prot_sub2
IPR017988Ribosome_inactivat_prot_CS
IPR017989Ribosome_inactivat_1/2
Vocabulary: Biological Process
TermDefinition
GO:0017148negative regulation of translation
Vocabulary: Molecular Function
TermDefinition
GO:0030598rRNA N-glycosylase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0017148 negative regulation of translation
biological_process GO:0009405 pathogenesis
cellular_component GO:0005575 cellular_component
molecular_function GO:0030598 rRNA N-glycosylase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G001130.1CmoCh08G001130.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 33..221
score: 1.6
IPR001574Ribosome-inactivating proteinunknownSSF56371Ribosome inactivating proteins (RIP)coord: 423..487
score: 3.01E-14coord: 25..265
score: 2.23
IPR016138Ribosome-inactivating protein, subdomain 1GENE3DG3DSA:3.40.420.10coord: 25..183
score: 2.9E-57coord: 423..487
score: 5.1
IPR016139Ribosome-inactivating protein, subdomain 2GENE3DG3DSA:4.10.470.10coord: 184..264
score: 1.2
IPR017988Ribosome-inactivating protein conserved sitePROSITEPS00275SHIGA_RICINcoord: 176..192
scor
IPR017989Ribosome-inactivating protein subgroupPRINTSPR00396SHIGARICINcoord: 82..97
score: 5.1E-34coord: 172..192
score: 5.1E-34coord: 143..157
score: 5.1E-34coord: 204..221
score: 5.1E-34coord: 35..48
score: 5.1

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmoCh08G001130CmoCh17G005500Cucurbita moschata (Rifu)cmocmoB323