CmaCh08G001070 (gene) Cucurbita maxima (Rimu)

NameCmaCh08G001070
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionRibosome inactivating protein
LocationCma_Chr08 : 571582 .. 572460 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAAACTCATAGCAATCACCTCTTTCTTAGTCGCCATCTCTCTTATCTCTCCCACTCTCAAGGCTGATATAACCTTCGATTTGTCCACAGCTACCAAAGCATCCTATTCAGCTTTCATAACCCAGCTCAGGAATGCTCTTCCAACCACAGCAACAGTGTGCAACATACCCCTGCTGCCTTCCACCGCATCGGGCTTAGGCTGGTTCACATTCTTCACCATCACCAATTATAACTACCAAACCATCACGGTCGCTGTAAACGTAACCAACGTCTACATCGTAGCGTATCGTGCCGATGCAAGCTCCTACTTTTTCGAAGACACTCCAGCTGAAGCCAAGCAACTCTTATTTCCAAATACACAAAAGGTAACACTTCCTTTTACGGGTAACTATGATAAGCTCCAAAGCATTGTAGGCAAATATAGAGATTTGATTGAGCTTGGAATCCCCGCCTTGAGCACTGCAGTTACCAACATGGTTTACTACAATAAACAACTTACTGCCGCGGCGCTTCTTGTGCTGATTCAGTCCAGTGCTGAAGCTGCGAGGTTTAAATATATTGAGCAACAGGTTGCTCAGCATATTAGCGATAAGTTTTATCCAAACCAAGCAGTGATAAGCTTAGAAAACAACTGGGGAGCTCTCTCCAAGCAAATCCAGATAGCAAATAGTACTGGAAGGGGACAATTCCAGAGCCCCGTTGAGCTTATAAGCCCTAATGGCACACGATTTAGTGTAACCAGTACATCAGCTGGGGTTGTTCAAAGCAATATCAAGCTCCTGCTGTACTACAAACTGAGCGTTGCTGATGAAAACGACAGCCCGACAAGAATGCCTGACAATATTGTTGAATATGGAACTCATGATTCTGTGTAG

mRNA sequence

ATGAACAAACTCATAGCAATCACCTCTTTCTTAGTCGCCATCTCTCTTATCTCTCCCACTCTCAAGGCTGATATAACCTTCGATTTGTCCACAGCTACCAAAGCATCCTATTCAGCTTTCATAACCCAGCTCAGGAATGCTCTTCCAACCACAGCAACAGTGTGCAACATACCCCTGCTGCCTTCCACCGCATCGGGCTTAGGCTGGTTCACATTCTTCACCATCACCAATTATAACTACCAAACCATCACGGTCGCTGTAAACGTAACCAACGTCTACATCGTAGCGTATCGTGCCGATGCAAGCTCCTACTTTTTCGAAGACACTCCAGCTGAAGCCAAGCAACTCTTATTTCCAAATACACAAAAGGTAACACTTCCTTTTACGGGTAACTATGATAAGCTCCAAAGCATTGTAGGCAAATATAGAGATTTGATTGAGCTTGGAATCCCCGCCTTGAGCACTGCAGTTACCAACATGGTTTACTACAATAAACAACTTACTGCCGCGGCGCTTCTTGTGCTGATTCAGTCCAGTGCTGAAGCTGCGAGGTTTAAATATATTGAGCAACAGGTTGCTCAGCATATTAGCGATAAGTTTTATCCAAACCAAGCAGTGATAAGCTTAGAAAACAACTGGGGAGCTCTCTCCAAGCAAATCCAGATAGCAAATAGTACTGGAAGGGGACAATTCCAGAGCCCCGTTGAGCTTATAAGCCCTAATGGCACACGATTTAGTGTAACCAGTACATCAGCTGGGGTTGTTCAAAGCAATATCAAGCTCCTGCTGTACTACAAACTGAGCGTTGCTGATGAAAACGACAGCCCGACAAGAATGCCTGACAATATTGTTGAATATGGAACTCATGATTCTGTGTAG

Coding sequence (CDS)

ATGAACAAACTCATAGCAATCACCTCTTTCTTAGTCGCCATCTCTCTTATCTCTCCCACTCTCAAGGCTGATATAACCTTCGATTTGTCCACAGCTACCAAAGCATCCTATTCAGCTTTCATAACCCAGCTCAGGAATGCTCTTCCAACCACAGCAACAGTGTGCAACATACCCCTGCTGCCTTCCACCGCATCGGGCTTAGGCTGGTTCACATTCTTCACCATCACCAATTATAACTACCAAACCATCACGGTCGCTGTAAACGTAACCAACGTCTACATCGTAGCGTATCGTGCCGATGCAAGCTCCTACTTTTTCGAAGACACTCCAGCTGAAGCCAAGCAACTCTTATTTCCAAATACACAAAAGGTAACACTTCCTTTTACGGGTAACTATGATAAGCTCCAAAGCATTGTAGGCAAATATAGAGATTTGATTGAGCTTGGAATCCCCGCCTTGAGCACTGCAGTTACCAACATGGTTTACTACAATAAACAACTTACTGCCGCGGCGCTTCTTGTGCTGATTCAGTCCAGTGCTGAAGCTGCGAGGTTTAAATATATTGAGCAACAGGTTGCTCAGCATATTAGCGATAAGTTTTATCCAAACCAAGCAGTGATAAGCTTAGAAAACAACTGGGGAGCTCTCTCCAAGCAAATCCAGATAGCAAATAGTACTGGAAGGGGACAATTCCAGAGCCCCGTTGAGCTTATAAGCCCTAATGGCACACGATTTAGTGTAACCAGTACATCAGCTGGGGTTGTTCAAAGCAATATCAAGCTCCTGCTGTACTACAAACTGAGCGTTGCTGATGAAAACGACAGCCCGACAAGAATGCCTGACAATATTGTTGAATATGGAACTCATGATTCTGTGTAG

Protein sequence

MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLLPSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPNTQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSAEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISPNGTRFSVTSTSAGVVQSNIKLLLYYKLSVADENDSPTRMPDNIVEYGTHDSV
BLAST of CmaCh08G001070 vs. Swiss-Prot
Match: RIP1_TRIAN (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina GN=TCA PE=1 SV=2)

HSP 1 Score: 396.0 bits (1016), Expect = 3.6e-109
Identity = 205/273 (75.09%), Postives = 235/273 (86.08%), Query Frame = 1

Query: 5   IAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLLPSTA 64
           +A++ F +AISL SPT   D++FDLSTATK SYS+FITQLR+ALPT  TVC IPLLPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPNTQKV 124
           SG  WF FF +TNYN +T+TVAVNVTNVYIVAYRADA SYFFEDTPAEA +L+F  T+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 TLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSAEAAR 184
            LP++GNYDKLQS+VGK RD+IELGIPALS+A+TNMVYY+ Q TAAALLVLIQ +AEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 FKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISPNGTR 244
           +KYIEQQV+ HIS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL +P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 FSVTSTSAGVVQSNIKLLLYYKLSVADENDSPT 278
           FSVT+TSAGVV+ NIKLLLYYK SV  E D PT
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPT 273

BLAST of CmaCh08G001070 vs. Swiss-Prot
Match: RIP3_MOMCH (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia GN=MAP30 PE=1 SV=2)

HSP 1 Score: 267.7 bits (683), Expect = 1.5e-70
Identity = 143/273 (52.38%), Postives = 195/273 (71.43%), Query Frame = 1

Query: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP +  V +IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120
            ST S    F    +T+Y Y+TI+VA++VTNVY+VAYR    SYFF+++P EA  +LF  
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180
           T+K+TLP+TGNY+ LQ+   K R+ I+LG+PALS+A+T + YYN Q   +ALLVLIQ++A
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240
           EAARFKYIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADEN 274
            G RF VT+  + VV+ NIKLLL  + S ADEN
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

BLAST of CmaCh08G001070 vs. Swiss-Prot
Match: RIP2_MOMBA (Ribosome-inactivating protein momordin II OS=Momordica balsamina PE=3 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.0e-70
Identity = 143/273 (52.38%), Postives = 195/273 (71.43%), Query Frame = 1

Query: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP +  V +IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120
            ST S    F    +T+Y Y+TI+VA++VTNVY+VAYR    SYFF+++P EA  +LF  
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180
           T+K+TLP+TGNY+ LQ+   K R+ I+LG+PALS+A+T + YYN Q   +ALLVLIQ++A
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240
           EAARFKYIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADEN 274
            G RF VT+  + VV+ NIKLLL  + S ADEN
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

BLAST of CmaCh08G001070 vs. Swiss-Prot
Match: RIP1_BRYDI (Ribosome-inactivating protein bryodin I OS=Bryonia dioica PE=1 SV=3)

HSP 1 Score: 245.7 bits (626), Expect = 6.1e-64
Identity = 142/265 (53.58%), Postives = 184/265 (69.43%), Query Frame = 1

Query: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60
           M KL+ +   ++ I L SPT++ D++F LS AT  SY  FI  LR ALP    V NIPLL
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLL 60

Query: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPA-EAKQLLFP 120
            S+ SG G +T   +TNY  +TI+VAV+VTNVYI+ Y A   SYFF +  A EA + +F 
Sbjct: 61  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFK 120

Query: 121 NTQK-VTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQS 180
           + +K VTLP++GNY++LQ+  GK R+ I LG+PAL +A+T + YY     A+ALLVLIQS
Sbjct: 121 DAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTASSAASALLVLIQS 180

Query: 181 SAEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELI 240
           +AE+AR+K+IEQQ+ + +   F P+ A ISLENNW ALSKQIQIA ST  GQF+SPV LI
Sbjct: 181 TAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIA-STNNGQFESPVVLI 240

Query: 241 SPNGTRFSVTSTSAGVVQSNIKLLL 264
             N  R S+T+ SA VV SNI LLL
Sbjct: 241 DGNNQRVSITNASARVVTSNIALLL 264

BLAST of CmaCh08G001070 vs. Swiss-Prot
Match: RIP_CUCMO (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata PE=1 SV=2)

HSP 1 Score: 240.0 bits (611), Expect = 3.3e-62
Identity = 126/240 (52.50%), Postives = 169/240 (70.42%), Query Frame = 1

Query: 24  DITFDLSTATKASYSAFITQLRNALPTTATVCNIPLLPSTASGLGWFTFFTITNYNYQTI 83
           ++ FDLS+AT +SY  FI  LR ALP    V +IP+L ST      F    + NY+ Q+I
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 60

Query: 84  TVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPNTQKVTLPFTGNYDKLQSIVGKYR 143
           T A++V NVYIVAY     SYFF+  PA+A +LLF  TQ+ TLP+TGNY+ LQ+   K R
Sbjct: 61  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 120

Query: 144 DLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSAEAARFKYIEQQVAQHISDKFYPN 203
           + IELG+PAL +A+T + +YN +  A+ALLVLIQ+++EAARF+YIE Q+A ++  KF P+
Sbjct: 121 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 180

Query: 204 QAVISLENNWGALSKQIQIANSTGRGQFQSPVELISPNGTRFSVTSTSAGVVQSNIKLLL 263
           Q +ISLENNW ALSKQIQIA +   GQF++PV LI P G R  +T+ ++ VV  NI+LLL
Sbjct: 181 QTIISLENNWSALSKQIQIAKNK-NGQFETPVILIDPQGNRVQITNVTSNVVTQNIQLLL 239

BLAST of CmaCh08G001070 vs. TrEMBL
Match: S6CNA0_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 9.8e-69
Identity = 142/267 (53.18%), Postives = 193/267 (72.28%), Query Frame = 1

Query: 7   ITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLLPSTASG 66
           ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP +  V +IPLL ST SG
Sbjct: 1   LSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISG 60

Query: 67  LGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPNTQKVTL 126
              F    +T+Y Y+TI+VA++VTNVY+VAYR    SYFF+++P EA  +LF  T+K+TL
Sbjct: 61  SRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITL 120

Query: 127 PFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSAEAARFK 186
           P+TGNY+ LQ+   K R+ I+LG+PALS+A+T + YYN Q   +ALLVLIQ++AEAARFK
Sbjct: 121 PYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTAEAARFK 180

Query: 187 YIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISPNGTRFS 246
           YIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P G RF 
Sbjct: 181 YIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQ 240

Query: 247 VTSTSAGVVQSNIKLLLYYKLSVADEN 274
           VT+  + VV+ NIKLLL  + S ADEN
Sbjct: 241 VTNVDSDVVKGNIKLLLNSRASTADEN 266

BLAST of CmaCh08G001070 vs. TrEMBL
Match: W0LQW1_MOMCH (rRNA N-glycosidase OS=Momordica charantia PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-68
Identity = 143/273 (52.38%), Postives = 195/273 (71.43%), Query Frame = 1

Query: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP +  V +IPLL
Sbjct: 1   MVKCLVLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIENFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120
            ST S    F    +T+Y Y+TI+VA++VTNVY+VAYR    SYFF+++P EA  +LF  
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180
           T+K+TLP+TGNY+ LQ+   K R+ I+LG+PALS+A+T + YYN Q   +ALLVLIQ++A
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240
           EAARFKYIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVARYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADEN 274
            G RF VT+  + VV+ NIKLLL  + S ADEN
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

BLAST of CmaCh08G001070 vs. TrEMBL
Match: Q5PZ05_MOMCH (rRNA N-glycosidase OS=Momordica charantia PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.7e-68
Identity = 143/273 (52.38%), Postives = 195/273 (71.43%), Query Frame = 1

Query: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP +  V +IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120
            ST S    F    +T+Y Y+TI+VA++VTNVY+VAYR    SYFF+++P EA  +LF  
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180
           T+K+TLP+TGNY+ LQ+   K R+ I+LG+PALS+A+T + YYN Q   +ALLVLIQ++A
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240
           EAARFKYIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADEN 274
            G RF VT+  + VV+ NIKLLL  + S ADEN
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

BLAST of CmaCh08G001070 vs. TrEMBL
Match: Q0ZBB8_MOMCH (rRNA N-glycosidase OS=Momordica charantia GN=MAP30 PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 4.8e-68
Identity = 142/273 (52.01%), Postives = 195/273 (71.43%), Query Frame = 1

Query: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP +  V +IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120
            ST S    F    +T+Y Y+TI+VA++VTNVY+VAYR    SYFF+++P EA  +LF  
Sbjct: 61  YSTISDSRHFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180
           T+K+TLP+TGNY+ LQ+   K R+ I+LG+PALS+A+T + YYN Q   +ALLVLIQ++A
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240
           EAARFKYIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADEN 274
            G RF VT+  + VV+ NIKLLL  + S ADE+
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADED 272

BLAST of CmaCh08G001070 vs. TrEMBL
Match: S6CM68_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 4.8e-68
Identity = 142/267 (53.18%), Postives = 192/267 (71.91%), Query Frame = 1

Query: 7   ITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLLPSTASG 66
           ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP +  V +IPLL ST S 
Sbjct: 1   LSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISD 60

Query: 67  LGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPNTQKVTL 126
              F    +T+Y Y+TI+VAV+VTNVY+VAYR    SYFF+++P EA  +LF  T+K+TL
Sbjct: 61  SRRFILLNLTSYAYETISVAVDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITL 120

Query: 127 PFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSAEAARFK 186
           P+TGNY+ LQ+   K R+ I+LG+PALS+A+T + YYN Q   +ALLVLIQ++AEAARFK
Sbjct: 121 PYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTAEAARFK 180

Query: 187 YIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISPNGTRFS 246
           YIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P G RF 
Sbjct: 181 YIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQ 240

Query: 247 VTSTSAGVVQSNIKLLLYYKLSVADEN 274
           VT+  + VV+ NIKLLL  + S ADEN
Sbjct: 241 VTNVDSDVVKGNIKLLLNSRASTADEN 266

BLAST of CmaCh08G001070 vs. NCBI nr
Match: gi|8247957|sp|P56626.2|RIP1_TRIAN (RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; AltName: Full=Trichoanguin; AltName: Full=rRNA N-glycosidase; Flags: Precursor)

HSP 1 Score: 396.0 bits (1016), Expect = 5.8e-107
Identity = 205/273 (75.09%), Postives = 235/273 (86.08%), Query Frame = 1

Query: 5   IAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLLPSTA 64
           +A++ F +AISL SPT   D++FDLSTATK SYS+FITQLR+ALPT  TVC IPLLPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPNTQKV 124
           SG  WF FF +TNYN +T+TVAVNVTNVYIVAYRADA SYFFEDTPAEA +L+F  T+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 TLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSAEAAR 184
            LP++GNYDKLQS+VGK RD+IELGIPALS+A+TNMVYY+ Q TAAALLVLIQ +AEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 FKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISPNGTR 244
           +KYIEQQV+ HIS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL +P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 FSVTSTSAGVVQSNIKLLLYYKLSVADENDSPT 278
           FSVT+TSAGVV+ NIKLLLYYK SV  E D PT
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPT 273

BLAST of CmaCh08G001070 vs. NCBI nr
Match: gi|530776695|emb|CCD28533.1| (ribosome inactivating protein, partial [Momordica charantia])

HSP 1 Score: 268.5 bits (685), Expect = 1.4e-68
Identity = 142/267 (53.18%), Postives = 193/267 (72.28%), Query Frame = 1

Query: 7   ITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLLPSTASG 66
           ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP +  V +IPLL ST SG
Sbjct: 1   LSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISG 60

Query: 67  LGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPNTQKVTL 126
              F    +T+Y Y+TI+VA++VTNVY+VAYR    SYFF+++P EA  +LF  T+K+TL
Sbjct: 61  SRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITL 120

Query: 127 PFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSAEAARFK 186
           P+TGNY+ LQ+   K R+ I+LG+PALS+A+T + YYN Q   +ALLVLIQ++AEAARFK
Sbjct: 121 PYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTAEAARFK 180

Query: 187 YIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISPNGTRFS 246
           YIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P G RF 
Sbjct: 181 YIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQ 240

Query: 247 VTSTSAGVVQSNIKLLLYYKLSVADEN 274
           VT+  + VV+ NIKLLL  + S ADEN
Sbjct: 241 VTNVDSDVVKGNIKLLLNSRASTADEN 266

BLAST of CmaCh08G001070 vs. NCBI nr
Match: gi|574452326|gb|AHG25320.1| (ribosome-inactivating protein [Momordica charantia])

HSP 1 Score: 268.1 bits (684), Expect = 1.8e-68
Identity = 143/273 (52.38%), Postives = 195/273 (71.43%), Query Frame = 1

Query: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP +  V +IPLL
Sbjct: 1   MVKCLVLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIENFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120
            ST S    F    +T+Y Y+TI+VA++VTNVY+VAYR    SYFF+++P EA  +LF  
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180
           T+K+TLP+TGNY+ LQ+   K R+ I+LG+PALS+A+T + YYN Q   +ALLVLIQ++A
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240
           EAARFKYIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVARYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADEN 274
            G RF VT+  + VV+ NIKLLL  + S ADEN
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

BLAST of CmaCh08G001070 vs. NCBI nr
Match: gi|46577684|sp|P24817.2|RIP3_MOMCH (RecName: Full=Ribosome-inactivating protein beta-momorcharin; Short=B-MMC; AltName: Full=MAP 30; AltName: Full=rRNA N-glycosidase; Flags: Precursor)

HSP 1 Score: 267.7 bits (683), Expect = 2.4e-68
Identity = 143/273 (52.38%), Postives = 195/273 (71.43%), Query Frame = 1

Query: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP +  V +IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120
            ST S    F    +T+Y Y+TI+VA++VTNVY+VAYR    SYFF+++P EA  +LF  
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180
           T+K+TLP+TGNY+ LQ+   K R+ I+LG+PALS+A+T + YYN Q   +ALLVLIQ++A
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240
           EAARFKYIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADEN 274
            G RF VT+  + VV+ NIKLLL  + S ADEN
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

BLAST of CmaCh08G001070 vs. NCBI nr
Match: gi|132581|sp|P29339.1|RIP2_MOMBA (RecName: Full=Ribosome-inactivating protein momordin II; AltName: Full=rRNA N-glycosidase; Flags: Precursor)

HSP 1 Score: 267.3 bits (682), Expect = 3.1e-68
Identity = 143/273 (52.38%), Postives = 195/273 (71.43%), Query Frame = 1

Query: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP +  V +IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120
            ST S    F    +T+Y Y+TI+VA++VTNVY+VAYR    SYFF+++P EA  +LF  
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180
           T+K+TLP+TGNY+ LQ+   K R+ I+LG+PALS+A+T + YYN Q   +ALLVLIQ++A
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240
           EAARFKYIE+ VA++++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADEN 274
            G RF VT+  + VV+ NIKLLL  + S ADEN
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RIP1_TRIAN3.6e-10975.09Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina GN=T... [more]
RIP3_MOMCH1.5e-7052.38Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia GN=MAP30 P... [more]
RIP2_MOMBA2.0e-7052.38Ribosome-inactivating protein momordin II OS=Momordica balsamina PE=3 SV=1[more]
RIP1_BRYDI6.1e-6453.58Ribosome-inactivating protein bryodin I OS=Bryonia dioica PE=1 SV=3[more]
RIP_CUCMO3.3e-6252.50Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata PE=1 SV=2[more]
Match NameE-valueIdentityDescription
S6CNA0_MOMCH9.8e-6953.18Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
W0LQW1_MOMCH1.3e-6852.38rRNA N-glycosidase OS=Momordica charantia PE=2 SV=1[more]
Q5PZ05_MOMCH1.7e-6852.38rRNA N-glycosidase OS=Momordica charantia PE=3 SV=1[more]
Q0ZBB8_MOMCH4.8e-6852.01rRNA N-glycosidase OS=Momordica charantia GN=MAP30 PE=3 SV=1[more]
S6CM68_MOMCH4.8e-6853.18Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
gi|8247957|sp|P56626.2|RIP1_TRIAN5.8e-10775.09RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; Alt... [more]
gi|530776695|emb|CCD28533.1|1.4e-6853.18ribosome inactivating protein, partial [Momordica charantia][more]
gi|574452326|gb|AHG25320.1|1.8e-6852.38ribosome-inactivating protein [Momordica charantia][more]
gi|46577684|sp|P24817.2|RIP3_MOMCH2.4e-6852.38RecName: Full=Ribosome-inactivating protein beta-momorcharin; Short=B-MMC; AltNa... [more]
gi|132581|sp|P29339.1|RIP2_MOMBA3.1e-6852.38RecName: Full=Ribosome-inactivating protein momordin II; AltName: Full=rRNA N-gl... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001574Ribosome_inactivat_prot
IPR016138Ribosome_inactivat_prot_sub1
IPR016139Ribosome_inactivat_prot_sub2
IPR017988Ribosome_inactivat_prot_CS
IPR017989Ribosome_inactivat_1/2
Vocabulary: Biological Process
TermDefinition
GO:0017148negative regulation of translation
Vocabulary: Molecular Function
TermDefinition
GO:0030598rRNA N-glycosylase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0017148 negative regulation of translation
cellular_component GO:0005575 cellular_component
molecular_function GO:0030598 rRNA N-glycosylase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G001070.1CmaCh08G001070.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 33..221
score: 1.3
IPR001574Ribosome-inactivating proteinunknownSSF56371Ribosome inactivating proteins (RIP)coord: 24..268
score: 2.1
IPR016138Ribosome-inactivating protein, subdomain 1GENE3DG3DSA:3.40.420.10coord: 24..183
score: 5.2
IPR016139Ribosome-inactivating protein, subdomain 2GENE3DG3DSA:4.10.470.10coord: 184..266
score: 2.8
IPR017988Ribosome-inactivating protein conserved sitePROSITEPS00275SHIGA_RICINcoord: 176..192
scor
IPR017989Ribosome-inactivating protein subgroupPRINTSPR00396SHIGARICINcoord: 82..97
score: 1.3E-33coord: 172..192
score: 1.3E-33coord: 143..157
score: 1.3E-33coord: 204..221
score: 1.3E-33coord: 35..48
score: 1.3

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh08G001070CmoCh08G001130Cucurbita moschata (Rifu)cmacmoB913
CmaCh08G001070Cp4.1LG17g10070Cucurbita pepo (Zucchini)cmacpeB914
The following gene(s) are paralogous to this gene:

None