Cp4.1LG09g05540 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG09g05540
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Calcium-binding EF-hand
Location	Cp4.1LG09 : 4612545 .. 4616278 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCGGAAGCCATCAGCGTTGCGTATAGAATCCGTAGAATTCTATTTCCGTCGGTTACTAAGACGAACATTTGAGATAAATGATCGCAAAACGAATACCCAAAATCCGCGTTATCATCTTCAATCCAATAACCTAAGCAACCGCGTATTCCATAATTCCTCTGCAATTTCCCCTTTCCTTCCACTTTTTTCCCCTGTTTTCACTTTAAATCCCACCCAGATTCTCATCGGATAAGCATCAGAACTCGCTTTTCCTGCCACCGTTTCAGATTTCATCCTTGAAAGAGAGATCCCAGATTCGAATCCTTCCCTGATTCAATTTTATTACCCATTTCCTCTCTTTTTTGGCCATCAATGGCGTCTTTCTTTGGTAGATTCACTTCCCATTCCAATTCCAATTCCCATTCCCATTCCCATTCCCATTCCCATTCCCATGCCCCATCGGCGCCATCAGCACCGTCCGAGCCGGAAACCTACGGCCATGGCAATTCTCACGGGACATCTTCGCCGTATTACTCCCAACAGCACCAGCCGCAGCCTTATGGGTCTAATTATGGTGGGGTTTCTTCATATGGGTCTTATGGCTTTCCGCCAGGGACAAGCCCTGAGGTGATTAGGAGCTTTCAAATGGTGGATAAGGATCGAAGTGGATTCATCGATGAGAATGAATTGCAGGAAGCCCTTTCTTCTGGCTACCAGAGGTTTAGTCTTAGAACCGTTCGTTTGCTCATTTTCTTGTTCCGAAATCCGATCGATTCCTCCAGAATGGGTGAGTTTCTTCTTGTTTTTTCTCTAATTTAATCCTCTTGGTTTATTTTGGATCATTGGTGCAATATTGGGTTTCTAAATTTTGCAATCATAATAAGTTAGTTATAATCGTTCTTTAGGCATTAATTTGTATATGTTTCTCCTTTTGGTTGCCTTTCTTGGAAGTGAAATGTTCTGTTTTGTGTTCTTGTCCTCTTTCCTTTAAAGGAATGTCAACCATGAACATAAAGTATTTGCCAATTATATTCAACTTGTTTTTAAAATATTCAAACATCGACTAAGTTTGGTGGTGCCTTTTCATTTAACAATGTGTGTCTGAATTGGCTGTTGCAGGGCCTAATGAGTTTACAGCTTTGTGGAACTGTCTTGGCCAATGGCGTGTAAGTAATGCGATTACTCTCTATCTTCTTCATGGTGCATGTTAGGAATCACGAACTTCTACAATCGTATGATATTGTTTACTTTGAACATAAGCTCTCATGGCTTTACTTTTGGTTTCTCGAAAAGGTCTCATATCAATAGAGATAGTATTCCTTACTTAGAAACTCAGGATCAACCCCTTAGTTAGCAGATGTTGGACTCCTCTTCCAACAATCCTTCCCTCGAACAAAATACACCATAGAGCCTCCCTTGAGGTCTATGGAGCCCACGAACAGCCTCCCCTTAATCGACTCCTTCTTTGGAGCTCATGGAACAATGTACACCATTTGTTTGACACTTGAGTCACTTTTGACTACACTTTTGAGGTTCAAAACTTTTTTGTTTGTTATTTGAGGATTCTATTGACATGGTTAAGTCTGATACTATGTTAGGAATCACGAACCTCCATAATGGTATGATATTGTCCACTTTGAGCATAAGCTCTCGTGGCTTTATTTTTGGTTTCTCCAAAAAGCCTCATAGTAATGGAGATAGTATTTCTTACTTATAAACTCATGATCAACCCCTTAATTAGCCAATGTGGATTTGTTTTTGCTCAGAGAAAGATGGTGGTAATGAAATTAGAACTTCGACTTGAAGTAAATGATTGGTTTCAAGTTTCACTGTGAGCTGTTTTGTTGTGATGAGTATTTGAATTAAAACCATGATAGGATTAGATACCAGAGAAGCATTTAAGCTGATTCAATATGTTGAAGCTAAAACTACACTAGATGCACATGTAGTCGTAGTAGTCGGCTCAGACGCGAGTCCGCTTTCTTTTTTAAGAACATCTACCATGTCTTGAATAGCTTACTACTTTCGGTTGTGGCCCGCCTCACTGACTCATACATTCCCCTAAAGAATGAGGCTAGAGCTATCTTACACTAATAAGAATCTCCAGCTCACCTAGCACCTATTAGTTCAATTCTTTTTCTTACACTAATAAGAATCTCCAGCTCACCTAGCACCTATTAGTTGAATTCTTTTTCTTAGATTTCGGAACGCTCTAAATAAGAATATGCACTCAGCTTAGGTAGGTCCATCTACTTCCGCACAATTTGCCCTCTTTTGGTGCACCACATACATAGAATATGCACTCAGCTTAGGTAGGTCCATCTACTTCCGCACAATTTGCCCTCTTTTGGTGCACCACCCATGTATGTGGTTTATCTCCAAAGAGTTCTCAAAACTTATATGATATCTTAGTTCAAAACTTGGTTACTTTTTTTCTTTCTTTTTTGGTGATTTATTTCTTCATGATTATCAATATTGTGCCCTAAGACTGAGAAAGAATAAGCAATTTGCAGGGCACCTTTGAGAGGTATGATAGAGATAGAAGTGGGAGAATTGATGCAATGGAAATGAGGGATGCACTCTATGGTCTTGGCTATGCAGTTCCTTCTTCAGTCCTTCAACTTCTAATTTCACTATACGATGATCGAAGTGGTCGTCGAGTTGAATATAATTTCGACAGTTTTGTCGAGTAAGTGTGTCAAATCGTTAAATATGTATTGCTTTTTATTTGCCCAAAAGCCTTTGATGCTTACATTTCCGGTATTGTTTTCTTTTTCATTGCAGGTGTGGCATGATAGTAAAGGTGAGATTGCAGTCCTTTTATTACTCCCTAATTCCTAATATCATCATGTTTACTCGACTATATAATACTCTAGATCGTTCTTGTCGAAGTAATCGAATATCGCTTTGTACGTGTGAACCAATGCCACGGAGTACCGCTCTTTAAGTAAAACTACAAGCGTTTTGTTCATGGTTACCAGTAGTTTTAGTATCATATTGTTCGTGATAGCACGTGCTTTCAAATGTAATATCGTTTCTAAACCCAATGCTTGGAAAATTTCAGGGGTTGACCGAGAAATTCAAGGAGAAGGATAGGCATTACACGGGCTCGGCAACCCTTACATACGAAGATTTCATGGCAACTATCCTCCCATTTCTTGTGTCATACAGTTGAAGCCCTCAAAGGTGATGAATATACGTCTTTATGGGTTGGATTTTGTGTAGTATCATCTCTTCATTTGTTGTTTACTTGAAATGGTAGAAGCTGTACATTCAGGGAAGCTGCTGTTTATGTTGGCGTGAGAGAGTTTCCATGTACAGATATTATATTCCTTTCATCTTTTTTGTTTCTTCTGTTTTGATTCTGCTGATTAGAATTTTTTATTTGTTGATTTGTTTAGATTATTTGTCACTGATTTTAATAAAGTTTTGGAACTTGTACAAGAATATGAAGGAATGGCTATGGTTTATTTGAAAGAGTGCAGTTTTTATGCAATTTCGTTAATCGTAAACGGTATGTATTGTTATCGTTGTTAGCCTCACGATTTTAAAATGCGGCTGGTAAAGAAAGGTTTTCAGACTCTTAAGAAATGTTTTGTTCTCATTTCCAACTGATGTGGGATCTCACAATCCACCCCCTTCGGAGCTAGCGTCCTCATTTGTACACTGCTCGATATCTGGCTTTAATACCATTTGTGACAGCTCAAACCCACCCACCGTTAACTTATATTGTCCGCTTTGATCCATTACATAT

mRNA sequence

CGCGGAAGCCATCAGCGTTGCGTATAGAATCCGTAGAATTCTATTTCCGTCGGTTACTAAGACGAACATTTGAGATAAATGATCGCAAAACGAATACCCAAAATCCGCGTTATCATCTTCAATCCAATAACCTAAGCAACCGCGTATTCCATAATTCCTCTGCAATTTCCCCTTTCCTTCCACTTTTTTCCCCTGTTTTCACTTTAAATCCCACCCAGATTCTCATCGGATAAGCATCAGAACTCGCTTTTCCTGCCACCGTTTCAGATTTCATCCTTGAAAGAGAGATCCCAGATTCGAATCCTTCCCTGATTCAATTTTATTACCCATTTCCTCTCTTTTTTGGCCATCAATGGCGTCTTTCTTTGGTAGATTCACTTCCCATTCCAATTCCAATTCCCATTCCCATTCCCATTCCCATTCCCATTCCCATGCCCCATCGGCGCCATCAGCACCGTCCGAGCCGGAAACCTACGGCCATGGCAATTCTCACGGGACATCTTCGCCGTATTACTCCCAACAGCACCAGCCGCAGCCTTATGGGTCTAATTATGGTGGGGTTTCTTCATATGGGTCTTATGGCTTTCCGCCAGGGACAAGCCCTGAGGTGATTAGGAGCTTTCAAATGGTGGATAAGGATCGAAGTGGATTCATCGATGAGAATGAATTGCAGGAAGCCCTTTCTTCTGGCTACCAGAGGTTTAGTCTTAGAACCGTTCGTTTGCTCATTTTCTTGTTCCGAAATCCGATCGATTCCTCCAGAATGGGTCCATCTACTTCCGCACAATTTGCCCTCTTTTGGTGCACCACCCATGGCACCTTTGAGAGGTATGATAGAGATAGAAGTGGGAGAATTGATGCAATGGAAATGAGGGATGCACTCTATGGTCTTGGCTATGCAGTTCCTTCTTCAGTCCTTCAACTTCTAATTTCACTATACGATGATCGAAGTGGTCGTCGAGTTGAATATAATTTCGACAGTTTTGTCGAGTGTGGCATGATAGTAAAGGGGTTGACCGAGAAATTCAAGGAGAAGGATAGGCATTACACGGGCTCGGCAACCCTTACATACGAAGATTTCATGGCAACTATCCTCCCATTTCTTGTGTCATACAGTTGAAGCCCTCAAAGGTGATGAATATACGTCTTTATGGGTTGGATTTTGTGTAGTATCATCTCTTCATTTGTTGTTTACTTGAAATGGTAGAAGCTGTACATTCAGGGAAGCTGCTGTTTATGTTGGCGTGAGAGAGTTTCCATGTACAGATATTATATTCCTTTCATCTTTTTTGTTTCTTCTGTTTTGATTCTGCTGATTAGAATTTTTTATTTGTTGATTTGTTTAGATTATTTGTCACTGATTTTAATAAAGTTTTGGAACTTGTACAAGAATATGAAGGAATGGCTATGGTTTATTTGAAAGAGTGCAGTTTTTATGCAATTTCGTTAATCGTAAACGGTATGTATTGTTATCGTTGTTAGCCTCACGATTTTAAAATGCGGCTGGTAAAGAAAGGTTTTCAGACTCTTAAGAAATGTTTTGTTCTCATTTCCAACTGATGTGGGATCTCACAATCCACCCCCTTCGGAGCTAGCGTCCTCATTTGTACACTGCTCGATATCTGGCTTTAATACCATTTGTGACAGCTCAAACCCACCCACCGTTAACTTATATTGTCCGCTTTGATCCATTACATAT

Coding sequence (CDS)

ATGGCGTCTTTCTTTGGTAGATTCACTTCCCATTCCAATTCCAATTCCCATTCCCATTCCCATTCCCATTCCCATTCCCATGCCCCATCGGCGCCATCAGCACCGTCCGAGCCGGAAACCTACGGCCATGGCAATTCTCACGGGACATCTTCGCCGTATTACTCCCAACAGCACCAGCCGCAGCCTTATGGGTCTAATTATGGTGGGGTTTCTTCATATGGGTCTTATGGCTTTCCGCCAGGGACAAGCCCTGAGGTGATTAGGAGCTTTCAAATGGTGGATAAGGATCGAAGTGGATTCATCGATGAGAATGAATTGCAGGAAGCCCTTTCTTCTGGCTACCAGAGGTTTAGTCTTAGAACCGTTCGTTTGCTCATTTTCTTGTTCCGAAATCCGATCGATTCCTCCAGAATGGGTCCATCTACTTCCGCACAATTTGCCCTCTTTTGGTGCACCACCCATGGCACCTTTGAGAGGTATGATAGAGATAGAAGTGGGAGAATTGATGCAATGGAAATGAGGGATGCACTCTATGGTCTTGGCTATGCAGTTCCTTCTTCAGTCCTTCAACTTCTAATTTCACTATACGATGATCGAAGTGGTCGTCGAGTTGAATATAATTTCGACAGTTTTGTCGAGTGTGGCATGATAGTAAAGGGGTTGACCGAGAAATTCAAGGAGAAGGATAGGCATTACACGGGCTCGGCAACCCTTACATACGAAGATTTCATGGCAACTATCCTCCCATTTCTTGTGTCATACAGTTGA

Protein sequence

MASFFGRFTSHSNSNSHSHSHSHSHSHAPSAPSAPSEPETYGHGNSHGTSSPYYSQQHQPQPYGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLIFLFRNPIDSSRMGPSTSAQFALFWCTTHGTFERYDRDRSGRIDAMEMRDALYGLGYAVPSSVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMATILPFLVSYS

Homology

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BLAST of Cp4.1LG09g05540 vs. Swiss-Prot
Match: CML48_ARATH (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana GN=CML48 PE=2 SV=2)

HSP 1 Score: 198.0 bits (502), Expect = 1.3e-49
Identity = 109/236 (46.19%), Postives = 156/236 (66.10%), Query Frame = 1


Query: 22  SHSHSHAPSAPSAPSEPETYGH-GNSHGTSSPYYSQQHQPQPYGSNYGGVSSYGSYGFPP 81
           S+S+++APSAP  P       H G S  T   Y    +QP    S+Y G+       F P
Sbjct: 2   SYSNAYAPSAPELPESFVQQQHDGESRYT---YAYPSYQPTQQFSSYSGM-------FSP 61

Query: 82  GTSPEVIRSFQMVDKDRSGFIDENELQEALS-SGYQRFSLRTVRLLIFLFRNPIDSS-RM 141
            T PE++RSF+  D++RSGF++E+EL++ALS SGY   S RT+RLL+F+++ P+DS  R+
Sbjct: 62  ETHPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRL 121

Query: 142 GPSTSAQFALFWCTTHGTFERYDRDRSGRIDAMEMRDALYGLGYAVPSSVLQLLISLYDD 201
           GP    +           F RYDRDRSG++++ ++RDA Y LG  +P+SV QL++S +DD
Sbjct: 122 GPKEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDD 181

Query: 202 RSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMATILPFLVSY 255
            +G+ V+  FDSF+ECGMIVKGLTEKF+E D  YTG ATL+Y+ FM  ++PF+ +Y
Sbjct: 182 GTGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFIATY 227

[Close]

BLAST of Cp4.1LG09g05540 vs. Swiss-Prot
Match: CML50_ARATH (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana GN=CML50 PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 4.1e-48
Identity = 113/241 (46.89%), Postives = 149/241 (61.83%), Query Frame = 1


Query: 35  PSEPETYGHGNSHGTSSPYYSQQH------QPQP----YGSNYGGV---SSYGS------ 94
           PS+P   GHG  +G + P+    +       P+P    +G  YGG    +SYGS      
Sbjct: 119 PSQPS--GHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPFASLI 178

Query: 95  -YGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLIFLFRNPID 154
             GF PGT P ++  FQ  D+D SGFID+ ELQ ALSS  QRFS+RTV LL++LF N  +
Sbjct: 179 PSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNS-N 238

Query: 155 SSRMGPSTSAQFALFWCTTH--GTFERYDRDRSGRIDAMEMRDALYGLGYAVPSSVLQLL 214
           + ++GP      ALF+   +    FER D+DRSGRID  E+RDAL  LG++V   VL LL
Sbjct: 239 AMKIGPKEFT--ALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLL 298

Query: 215 ISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMATILPFLV 254
           +S +D   G+     +D+F+EC + VKGLTEKFKEKD  Y+GSAT  YE FM T+LPFL+
Sbjct: 299 VSKFDKSGGKNRAIEYDNFIECCLTVKGLTEKFKEKDTAYSGSATFNYESFMLTVLPFLI 354

[Close]

BLAST of Cp4.1LG09g05540 vs. Swiss-Prot
Match: CML49_ARATH (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana GN=CML49 PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 8.5e-46
Identity = 106/242 (43.80%), Postives = 137/242 (56.61%), Query Frame = 1


Query: 26  SHAPSAPSA----PSEPETYGHGNSHGTSSPYYSQQHQPQPYGSNYGGVSSYGSYG---- 85
           +H  S PS        P+  GHG  +G +        Q   +G  YG      SYG    
Sbjct: 102 AHPSSGPSDYGGYGGAPQQSGHGGGYGGAP-------QQSGHGGGYGAPPPQASYGSPFA 161

Query: 86  ------FPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLIFLFRN 145
                 FPPGT P ++  FQ  D+D SGFID+ ELQ ALSS  Q FS+RTV LL++LF N
Sbjct: 162 SLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTN 221

Query: 146 PIDSSRMGPSTSAQFALFWCTTHGTFERYDRDRSGRIDAMEMRDALYGLGYAVPSSVLQL 205
             +  ++GP                FER+D+DRSGRID  E+RDAL  LG++V   +L L
Sbjct: 222 S-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDL 281

Query: 206 LISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMATILPFL 254
           L+S +D   GR     +D+F+EC + VKGLTEKFKEKD   +GSA   YE+FM T+LPFL
Sbjct: 282 LVSKFDKSGGRNRAIEYDNFIECCLTVKGLTEKFKEKDTALSGSAIFNYENFMLTVLPFL 335

[Close]

BLAST of Cp4.1LG09g05540 vs. Swiss-Prot
Match: PEF1_DANRE (Peflin OS=Danio rerio GN=pef1 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.7e-22
Identity = 75/244 (30.74%), Postives = 115/244 (47.13%), Query Frame = 1


Query: 32  PSAPSEPETYGH---GNSHGTSSPY--YSQQHQPQPYGSNYGGVSSYGSYG--------- 91
           P+A      YG    G  +G  SPY  Y Q     PYG        YG YG         
Sbjct: 31  PAAGQYGSPYGSAPPGQQYGGGSPYGSYGQPGPRAPYGGGQAPGGPYGGYGQPQGGPYRQ 90

Query: 92  ------FPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLIFLFRN 151
                  PPG +PE  + F  VD D+SG+I+  EL++AL + +   S      ++ L  N
Sbjct: 91  QGSAGNVPPGVNPEAYQWFSTVDSDQSGYINAKELKQALMN-FNNSSFNDETCIMML--N 150

Query: 152 PIDSSRMGPSTSAQFALFWCTTH---GTFERYDRDRSGRIDAMEMRDALYGLGYAVPSSV 211
             D ++ G      F+  W         F+++DRDRSG I+  EM  AL  +GY +    
Sbjct: 151 MFDKTKSGRVDVFGFSALWTFLQQWRAAFQQFDRDRSGSINTNEMHQALSQMGYNLSPQF 210

Query: 212 LQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMATIL 253
           +Q L++ Y  R G  V    D F++    ++ +T+ F+EKD   TG+  ++YEDF+++ +
Sbjct: 211 IQELVNRYSVRGGTGV-LQLDRFIQVCTQLQSMTQAFREKDTGMTGNVRMSYEDFLSSAI 270

[Close]

BLAST of Cp4.1LG09g05540 vs. Swiss-Prot
Match: PEF1_XENLA (Peflin OS=Xenopus laevis GN=pef1 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 5.0e-22
Identity = 77/230 (33.48%), Postives = 114/230 (49.57%), Query Frame = 1


Query: 19  HSHSHSHSHAPSAPSAPSEPETYGHGNSHGTSSPYYSQQHQPQPYGSNYGGVSSYGSYGF 78
           + H    S AP AP  P   +  G   S   S+PY SQQH     G+  G +        
Sbjct: 59  YGHPVPGSAAPGAPGGPYGGQAPGGPYSVPGSTPYGSQQHGSYGQGAPAGNI-------- 118

Query: 79  PPGTSPEVIRSFQMVDKDRSGFIDENELQEAL-SSGYQRFSLRTVRLLIFLFRNPIDSSR 138
           PPG  PE    FQ VD D SG+I   EL++AL ++ +  F+  T  +++ +F    D S 
Sbjct: 119 PPGVDPEAFSWFQTVDTDHSGYISLKELKQALVNTNWSSFNDETCTMMMNMF----DKSN 178

Query: 139 MGPSTSAQFALFWCTTH---GTFERYDRDRSGRIDAMEMRDALYGLGYAVPSSVLQLLIS 198
            G      F+  W         F++YDRDRSG I+  E+  AL  +GY +    +Q+++S
Sbjct: 179 SGRIDMFGFSALWRFIQQWRNLFQQYDRDRSGSINQGELHQALCQMGYQLSPQFVQIVMS 238

Query: 199 LYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 245
            Y  RS  +     D F++    ++ +TE F+EKD    G+A L+YEDF+
Sbjct: 239 RYAQRSA-QPGLQLDRFIQICTQLQSMTEAFREKDTGQIGTAKLSYEDFI 275

[Close]

BLAST of Cp4.1LG09g05540 vs. TrEMBL
Match: A0A0A0LT26_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G164670 PE=4 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 3.4e-110
Identity = 213/258 (82.56%), Postives = 233/258 (90.31%), Query Frame = 1


Query: 1   MASFFGRFTSHSNSNSHSHSHSHSHSHAPSAPSAPSEPETYGHGNSHGTSSPYYSQQHQP 60
           MASFF RFTS+SNS+S+S+S+SH    APSAPS PS PET G  +SH T+SPY+S+Q+QP
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSH----APSAPSPPSAPETQGQEHSHATASPYHSEQNQP 60

Query: 61  QPYGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLR 120
           QPYG NYGGVSSYGSYGFPPGTSPEVIRSFQMVD+DRSGFIDENELQ+ALSSGYQRFSLR
Sbjct: 61  QPYGFNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDRSGFIDENELQQALSSGYQRFSLR 120

Query: 121 TVRLLIFLFRNPIDSSRMGPSTSAQFALFW-C--TTHGTFERYDRDRSGRIDAMEMRDAL 180
           TVRLLIFLFRNPIDSSRMGP+   +F   W C     G FERYDRDRSGRIDA+EMRDAL
Sbjct: 121 TVRLLIFLFRNPIDSSRMGPN---EFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDAL 180

Query: 181 YGLGYAVPSSVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSAT 240
           YGLGYAVPSSVLQLLISLYDDRSG++VE+NFDSFVECGMIVKGLTEKFKEKDR+YTGSAT
Sbjct: 181 YGLGYAVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSAT 240

Query: 241 LTYEDFMATILPFLVSYS 256
           LTYEDFM+TILPFLVSYS
Sbjct: 241 LTYEDFMSTILPFLVSYS 251

[Close]

BLAST of Cp4.1LG09g05540 vs. TrEMBL
Match: B9HQ10_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s16510g PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 5.5e-76
Identity = 159/257 (61.87%), Postives = 193/257 (75.10%), Query Frame = 1


Query: 3   SFFGRFTSHSNSNSHSHSHSHSHSHAPSAPSAPSEPETYGH-GNSHGTS-SPYYSQQHQP 62
           SFF +    S+S+S S S+         APSAPS PE+Y   G ++ TS    +SQQ Q 
Sbjct: 2   SFFSKKPRSSSSSSSSSSY---------APSAPSLPESYDQRGQAYSTSHQSNHSQQQQQ 61

Query: 63  QPYGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLR 122
             YG   GG SSYG  GFPPGTSP+VIRSF+MVD+DRSGFIDENELQ+A+SSGYQRFS+R
Sbjct: 62  SYYGQGNGGGSSYGHSGFPPGTSPDVIRSFEMVDRDRSGFIDENELQQAVSSGYQRFSIR 121

Query: 123 TVRLLIFLFRNPIDSSRMGPSTSAQFALFW-C--TTHGTFERYDRDRSGRIDAMEMRDAL 182
           T+RLL+FLF+NP D  R GP    +FA  W C     G FERYD+DRSG+ID  E+RDAL
Sbjct: 122 TIRLLMFLFKNPHDPLRFGPK---EFAALWGCLGQWRGIFERYDKDRSGKIDLFELRDAL 181

Query: 183 YGLGYAVPSSVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSAT 242
           Y LG+A+PSSVLQ+LIS YDD SGRR+E NFDSFVECGMI+KGLTEKFKEKD+ +TG+ T
Sbjct: 182 YSLGFAIPSSVLQVLISKYDDGSGRRIELNFDSFVECGMILKGLTEKFKEKDKRHTGTTT 241

Query: 243 LTYEDFMATILPFLVSY 255
             Y++FM+ ++PFLVSY
Sbjct: 242 FNYDEFMSMVIPFLVSY 246

[Close]

BLAST of Cp4.1LG09g05540 vs. TrEMBL
Match: A0A067F4F6_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g025714mg PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 7.4e-73
Identity = 156/251 (62.15%), Postives = 187/251 (74.50%), Query Frame = 1


Query: 15  NSHSHSHSHSHSHAPSAPSAPSEPETYGHGNSHGTSSPYYSQQHQP------QPYGS-NY 74
           +++S S+S S S+APSAPS P       + NS    S YY+Q   P      QPY + +Y
Sbjct: 2   SNYSGSYSSSQSYAPSAPSLPETHNNSSYNNSSSAQS-YYAQPPPPPPPSQQQPYPAPSY 61

Query: 75  GGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLIF 134
           G  S+YG   FPPGT P+VIRSF+MVD+DRSGFIDENELQ+ALSSGYQRFSL T+RLL+F
Sbjct: 62  GQFSAYGHSAFPPGTHPDVIRSFEMVDRDRSGFIDENELQQALSSGYQRFSLSTIRLLMF 121

Query: 135 LFRNPIDSSRMGPSTSAQFALFWCTT---HGTFERYDRDRSGRIDAMEMRDALYGLGYAV 194
           LFRNP DS R+GP    +FA  W         FERYDRDRSG+ID ME+RDALY +GYAV
Sbjct: 122 LFRNPHDSLRIGPK---EFADLWSCLGQWRAIFERYDRDRSGKIDLMELRDALYSIGYAV 181

Query: 195 PSSVLQLLISLYDDRSG-RRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDF 254
           P SVLQLL+  YD+RSG R++  +FDSFVECGM+VKGLTEKFKEKD  YTGSATLTYE F
Sbjct: 182 PPSVLQLLMDKYDNRSGSRKLGLSFDSFVECGMVVKGLTEKFKEKDPRYTGSATLTYESF 241

[Close]

BLAST of Cp4.1LG09g05540 vs. TrEMBL
Match: W9QJD2_9ROSA (Putative calcium-binding protein CML48 OS=Morus notabilis GN=L484_022335 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 4.8e-72
Identity = 154/246 (62.60%), Postives = 181/246 (73.58%), Query Frame = 1


Query: 15  NSHSHSHSHSHSHAPSAPS-APSEPETYGHGNSHGTSSPY-YSQQHQPQPYGSNYGGVSS 74
           NSH H H+  HSH  S  S A  +  +  HG    +SSPY Y Q      + S+YG    
Sbjct: 37  NSHGHDHTQFHSHGSSPSSYAYGQSSSSSHGYGQSSSSPYGYGQMGSTSSFSSSYG---- 96

Query: 75  YGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLIFLFRNP 134
           YG  GFP GTSP+VIRSF+MVD+DRSGFID+NELQ+ALSSGYQ+FSLRT+RLLI LF+NP
Sbjct: 97  YGYVGFPAGTSPDVIRSFEMVDRDRSGFIDDNELQQALSSGYQKFSLRTIRLLISLFKNP 156

Query: 135 IDSS-RMGPSTSAQFALFWCTT---HGTFERYDRDRSGRIDAMEMRDALYGLGYAVPSSV 194
            D + R+GP    +FA  W       G F+RYD+DRSGRID ME RDALYGLGY VP SV
Sbjct: 157 NDPAPRIGPK---EFAGLWNCLGQWRGIFQRYDKDRSGRIDVMEFRDALYGLGYMVPGSV 216

Query: 195 LQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMATIL 254
           LQLLIS YD+ SGR+VE +FDSFVECGMIVKGLTEKFKEKD  Y+GSAT TY+ FM+ ++
Sbjct: 217 LQLLISKYDNGSGRKVELDFDSFVECGMIVKGLTEKFKEKDPRYSGSATFTYDAFMSMVI 275

[Close]

BLAST of Cp4.1LG09g05540 vs. TrEMBL
Match: V4UDU3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10029136mg PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 6.3e-72
Identity = 154/251 (61.35%), Postives = 187/251 (74.50%), Query Frame = 1


Query: 15  NSHSHSHSHSHSHAPSAPSAPSEPETYGHGNSHGTSSPYYSQQHQP------QPYGS-NY 74
           +++S S+S S S+APSAPS P       + NS    S YY+Q   P      QPY + ++
Sbjct: 2   SNYSGSYSSSQSYAPSAPSLPETHNNSSYNNSSSAQS-YYAQPPPPPPPSQQQPYPAPSH 61

Query: 75  GGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLIF 134
           G  S+YG   FPPGT P+VIRSF+MVD+DRSGFIDENELQ+ALSSGYQRFSL T+RLL+F
Sbjct: 62  GQFSAYGHSAFPPGTHPDVIRSFEMVDRDRSGFIDENELQQALSSGYQRFSLSTIRLLMF 121

Query: 135 LFRNPIDSSRMGPSTSAQFALFWCTT---HGTFERYDRDRSGRIDAMEMRDALYGLGYAV 194
           LF+NP DS R+GP    +FA  W         FERYDRDRSG+ID ME+RDALY +GYAV
Sbjct: 122 LFKNPHDSLRIGPK---EFADLWSCLGQWRAIFERYDRDRSGKIDLMELRDALYSIGYAV 181

Query: 195 PSSVLQLLISLYDDRSG-RRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDF 254
           P SVLQLL+  YD+RSG R++  +FDSFVECGM+VKGLTEKFKEKD  YTGSATLTYE F
Sbjct: 182 PPSVLQLLMDKYDNRSGSRKLGLSFDSFVECGMVVKGLTEKFKEKDPRYTGSATLTYESF 241

[Close]

BLAST of Cp4.1LG09g05540 vs. TAIR10
Match: AT2G27480.1 (AT2G27480.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 198.0 bits (502), Expect = 7.2e-51
Identity = 109/236 (46.19%), Postives = 156/236 (66.10%), Query Frame = 1


Query: 22  SHSHSHAPSAPSAPSEPETYGH-GNSHGTSSPYYSQQHQPQPYGSNYGGVSSYGSYGFPP 81
           S+S+++APSAP  P       H G S  T   Y    +QP    S+Y G+       F P
Sbjct: 2   SYSNAYAPSAPELPESFVQQQHDGESRYT---YAYPSYQPTQQFSSYSGM-------FSP 61

Query: 82  GTSPEVIRSFQMVDKDRSGFIDENELQEALS-SGYQRFSLRTVRLLIFLFRNPIDSS-RM 141
            T PE++RSF+  D++RSGF++E+EL++ALS SGY   S RT+RLL+F+++ P+DS  R+
Sbjct: 62  ETHPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRL 121

Query: 142 GPSTSAQFALFWCTTHGTFERYDRDRSGRIDAMEMRDALYGLGYAVPSSVLQLLISLYDD 201
           GP    +           F RYDRDRSG++++ ++RDA Y LG  +P+SV QL++S +DD
Sbjct: 122 GPKEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDD 181

Query: 202 RSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMATILPFLVSY 255
            +G+ V+  FDSF+ECGMIVKGLTEKF+E D  YTG ATL+Y+ FM  ++PF+ +Y
Sbjct: 182 GTGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFIATY 227

[Close]

BLAST of Cp4.1LG09g05540 vs. TAIR10
Match: AT5G04170.1 (AT5G04170.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 193.0 bits (489), Expect = 2.3e-49
Identity = 113/241 (46.89%), Postives = 149/241 (61.83%), Query Frame = 1


Query: 35  PSEPETYGHGNSHGTSSPYYSQQH------QPQP----YGSNYGGV---SSYGS------ 94
           PS+P   GHG  +G + P+    +       P+P    +G  YGG    +SYGS      
Sbjct: 119 PSQPS--GHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPFASLI 178

Query: 95  -YGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLIFLFRNPID 154
             GF PGT P ++  FQ  D+D SGFID+ ELQ ALSS  QRFS+RTV LL++LF N  +
Sbjct: 179 PSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNS-N 238

Query: 155 SSRMGPSTSAQFALFWCTTH--GTFERYDRDRSGRIDAMEMRDALYGLGYAVPSSVLQLL 214
           + ++GP      ALF+   +    FER D+DRSGRID  E+RDAL  LG++V   VL LL
Sbjct: 239 AMKIGPKEFT--ALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLL 298

Query: 215 ISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMATILPFLV 254
           +S +D   G+     +D+F+EC + VKGLTEKFKEKD  Y+GSAT  YE FM T+LPFL+
Sbjct: 299 VSKFDKSGGKNRAIEYDNFIECCLTVKGLTEKFKEKDTAYSGSATFNYESFMLTVLPFLI 354

[Close]

BLAST of Cp4.1LG09g05540 vs. TAIR10
Match: AT3G10300.3 (AT3G10300.3 Calcium-binding EF-hand family protein)

HSP 1 Score: 185.3 bits (469), Expect = 4.8e-47
Identity = 106/242 (43.80%), Postives = 137/242 (56.61%), Query Frame = 1


Query: 26  SHAPSAPSA----PSEPETYGHGNSHGTSSPYYSQQHQPQPYGSNYGGVSSYGSYG---- 85
           +H  S PS        P+  GHG  +G +        Q   +G  YG      SYG    
Sbjct: 102 AHPSSGPSDYGGYGGAPQQSGHGGGYGGAP-------QQSGHGGGYGAPPPQASYGSPFA 161

Query: 86  ------FPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLIFLFRN 145
                 FPPGT P ++  FQ  D+D SGFID+ ELQ ALSS  Q FS+RTV LL++LF N
Sbjct: 162 SLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTN 221

Query: 146 PIDSSRMGPSTSAQFALFWCTTHGTFERYDRDRSGRIDAMEMRDALYGLGYAVPSSVLQL 205
             +  ++GP                FER+D+DRSGRID  E+RDAL  LG++V   +L L
Sbjct: 222 S-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDL 281

Query: 206 LISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMATILPFL 254
           L+S +D   GR     +D+F+EC + VKGLTEKFKEKD   +GSA   YE+FM T+LPFL
Sbjct: 282 LVSKFDKSGGRNRAIEYDNFIECCLTVKGLTEKFKEKDTALSGSAIFNYENFMLTVLPFL 335

[Close]

BLAST of Cp4.1LG09g05540 vs. NCBI nr
Match: gi|659071684|ref|XP_008461406.1| (PREDICTED: probable calcium-binding protein CML48 [Cucumis melo])

HSP 1 Score: 414.1 bits (1063), Expect = 1.8e-112
Identity = 217/258 (84.11%), Postives = 236/258 (91.47%), Query Frame = 1


Query: 1   MASFFGRFTSHSNSNSHSHSHSHSHSHAPSAPSAPSEPETYGHGNSHGTSSPYYSQQHQP 60
           MASFF RFTS+SNS+S+S+S+S+S  HAPSAPSAPS PET GH  SH T+SPYYS+QHQP
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSNS--HAPSAPSAPSAPETQGHELSHATASPYYSEQHQP 60

Query: 61  QPYGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLR 120
           QPYGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVD+D SGFIDENELQ+ALSSGYQRFSLR
Sbjct: 61  QPYGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLR 120

Query: 121 TVRLLIFLFRNPIDSSRMGPSTSAQFALFW-C--TTHGTFERYDRDRSGRIDAMEMRDAL 180
           TVRLLIFLFRNPIDSSRMGP+   +F   W C     G FERYDRDRSGRIDA+EMRDAL
Sbjct: 121 TVRLLIFLFRNPIDSSRMGPN---EFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDAL 180

Query: 181 YGLGYAVPSSVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSAT 240
           YGLGYAVPSSVLQLLISLYDDRSG++VE+NFDSFVECGMIVKGLTEKFKEKDR+YTGSAT
Sbjct: 181 YGLGYAVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSAT 240

Query: 241 LTYEDFMATILPFLVSYS 256
           LTYEDFM+TILPFLVSY+
Sbjct: 241 LTYEDFMSTILPFLVSYN 253

[Close]

BLAST of Cp4.1LG09g05540 vs. NCBI nr
Match: gi|449443448|ref|XP_004139489.1| (PREDICTED: probable calcium-binding protein CML48 [Cucumis sativus])

HSP 1 Score: 406.0 bits (1042), Expect = 4.9e-110
Identity = 213/258 (82.56%), Postives = 233/258 (90.31%), Query Frame = 1


Query: 1   MASFFGRFTSHSNSNSHSHSHSHSHSHAPSAPSAPSEPETYGHGNSHGTSSPYYSQQHQP 60
           MASFF RFTS+SNS+S+S+S+SH    APSAPS PS PET G  +SH T+SPY+S+Q+QP
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSH----APSAPSPPSAPETQGQEHSHATASPYHSEQNQP 60

Query: 61  QPYGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLR 120
           QPYG NYGGVSSYGSYGFPPGTSPEVIRSFQMVD+DRSGFIDENELQ+ALSSGYQRFSLR
Sbjct: 61  QPYGFNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDRSGFIDENELQQALSSGYQRFSLR 120

Query: 121 TVRLLIFLFRNPIDSSRMGPSTSAQFALFW-C--TTHGTFERYDRDRSGRIDAMEMRDAL 180
           TVRLLIFLFRNPIDSSRMGP+   +F   W C     G FERYDRDRSGRIDA+EMRDAL
Sbjct: 121 TVRLLIFLFRNPIDSSRMGPN---EFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDAL 180

Query: 181 YGLGYAVPSSVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSAT 240
           YGLGYAVPSSVLQLLISLYDDRSG++VE+NFDSFVECGMIVKGLTEKFKEKDR+YTGSAT
Sbjct: 181 YGLGYAVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSAT 240

Query: 241 LTYEDFMATILPFLVSYS 256
           LTYEDFM+TILPFLVSYS
Sbjct: 241 LTYEDFMSTILPFLVSYS 251

[Close]

BLAST of Cp4.1LG09g05540 vs. NCBI nr
Match: gi|224104857|ref|XP_002313594.1| (hypothetical protein POPTR_0009s16510g [Populus trichocarpa])

HSP 1 Score: 292.4 bits (747), Expect = 7.9e-76
Identity = 159/257 (61.87%), Postives = 193/257 (75.10%), Query Frame = 1


Query: 3   SFFGRFTSHSNSNSHSHSHSHSHSHAPSAPSAPSEPETYGH-GNSHGTS-SPYYSQQHQP 62
           SFF +    S+S+S S S+         APSAPS PE+Y   G ++ TS    +SQQ Q 
Sbjct: 2   SFFSKKPRSSSSSSSSSSY---------APSAPSLPESYDQRGQAYSTSHQSNHSQQQQQ 61

Query: 63  QPYGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLR 122
             YG   GG SSYG  GFPPGTSP+VIRSF+MVD+DRSGFIDENELQ+A+SSGYQRFS+R
Sbjct: 62  SYYGQGNGGGSSYGHSGFPPGTSPDVIRSFEMVDRDRSGFIDENELQQAVSSGYQRFSIR 121

Query: 123 TVRLLIFLFRNPIDSSRMGPSTSAQFALFW-C--TTHGTFERYDRDRSGRIDAMEMRDAL 182
           T+RLL+FLF+NP D  R GP    +FA  W C     G FERYD+DRSG+ID  E+RDAL
Sbjct: 122 TIRLLMFLFKNPHDPLRFGPK---EFAALWGCLGQWRGIFERYDKDRSGKIDLFELRDAL 181

Query: 183 YGLGYAVPSSVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSAT 242
           Y LG+A+PSSVLQ+LIS YDD SGRR+E NFDSFVECGMI+KGLTEKFKEKD+ +TG+ T
Sbjct: 182 YSLGFAIPSSVLQVLISKYDDGSGRRIELNFDSFVECGMILKGLTEKFKEKDKRHTGTTT 241

Query: 243 LTYEDFMATILPFLVSY 255
             Y++FM+ ++PFLVSY
Sbjct: 242 FNYDEFMSMVIPFLVSY 246

[Close]

BLAST of Cp4.1LG09g05540 vs. NCBI nr
Match: gi|694430513|ref|XP_009342731.1| (PREDICTED: probable calcium-binding protein CML48 [Pyrus x bretschneideri])

HSP 1 Score: 290.8 bits (743), Expect = 2.3e-75
Identity = 158/249 (63.45%), Postives = 187/249 (75.10%), Query Frame = 1


Query: 29  PSAPSAPSEPETYG-------HG-------NSHGTSSPYYSQ---QHQPQPYGSNYGGVS 88
           P APSAPS PE+YG       HG       ++  +SSP YS    Q Q QPYGS++   S
Sbjct: 11  PYAPSAPSLPESYGQQHTQYPHGYGWPQQQHNSSSSSPQYSHGYGQPQHQPYGSSFSSSS 70

Query: 89  S--YGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLIFLF 148
           S  YG  GFP GT P VIRSF+MVD+DRSG+IDE+ELQ+ALSSGYQRFSLRT+RLLIFLF
Sbjct: 71  SSSYGQSGFPAGTDPAVIRSFEMVDRDRSGYIDEHELQQALSSGYQRFSLRTIRLLIFLF 130

Query: 149 RNPIDSSRMGPSTSAQFALFWCTT---HGTFERYDRDRSGRIDAMEMRDALYGLGYAVPS 208
           + P DS R+GP    +FA  W         FERYD+DRSG+ID+ME+RDALYGLG A+P 
Sbjct: 131 KCPTDSLRVGPK---EFAALWICLGQWRSIFERYDKDRSGKIDSMELRDALYGLGLAMPP 190

Query: 209 SVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMAT 256
           SVLQLLIS YDD SGRRVE NFDSFVECGMIV+GLT++FKEKDR YTGSAT  Y++FM+ 
Sbjct: 191 SVLQLLISKYDDGSGRRVELNFDSFVECGMIVRGLTDRFKEKDRRYTGSATFGYDEFMSM 250

[Close]

BLAST of Cp4.1LG09g05540 vs. NCBI nr
Match: gi|743791922|ref|XP_011043032.1| (PREDICTED: probable calcium-binding protein CML48 [Populus euphratica])

HSP 1 Score: 289.3 bits (739), Expect = 6.7e-75
Identity = 150/236 (63.56%), Postives = 178/236 (75.42%), Query Frame = 1


Query: 22  SHSHSHAPSAPSAPSEPETYGHGNSHGTSSPYYSQQHQPQPYGSNYGGVSSYGSYGFPPG 81
           S S SHAPSAPS P   +  G   S    S +  QQ Q   YG   GG SSYG  GFPPG
Sbjct: 10  SSSSSHAPSAPSLPDSYDQRGQAYSTSHQSNHSHQQQQQSYYGQGNGGSSSYGHSGFPPG 69

Query: 82  TSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLIFLFRNPIDSSRMGPS 141
           TSP+VIRSF+MVD+DRSGFIDENELQ+A+SSGYQRFS+RT+RLL+FLF+NP D  R GP 
Sbjct: 70  TSPDVIRSFEMVDRDRSGFIDENELQQAISSGYQRFSIRTIRLLMFLFKNPHDPLRCGPK 129

Query: 142 TSAQFALFW-C--TTHGTFERYDRDRSGRIDAMEMRDALYGLGYAVPSSVLQLLISLYDD 201
              +FA  W C     G FERYD+DRSG+ID  E+RDALY LG A+PSSVLQ+LIS YDD
Sbjct: 130 ---EFAALWGCLGQWRGIFERYDKDRSGKIDLFELRDALYSLGLAIPSSVLQVLISKYDD 189

Query: 202 RSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMATILPFLVSY 255
            SG+R+E NFDSFVECGMI+KGLTEKFKEKD+ +TG+ T  Y++ M+ ++PFLVSY
Sbjct: 190 GSGQRIELNFDSFVECGMILKGLTEKFKEKDKRHTGTTTFNYDELMSMVIPFLVSY 242

The following BLAST results are available for this feature:

BLAST of Cp4.1LG09g05540 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML48_ARATH	1.3e-49	46.19	Probable calcium-binding protein CML48 OS=Arabidopsis thaliana GN=CML48 PE=2 SV=...	[more]
CML50_ARATH	4.1e-48	46.89	Probable calcium-binding protein CML50 OS=Arabidopsis thaliana GN=CML50 PE=2 SV=...	[more]
CML49_ARATH	8.5e-46	43.80	Probable calcium-binding protein CML49 OS=Arabidopsis thaliana GN=CML49 PE=2 SV=...	[more]
PEF1_DANRE	1.7e-22	30.74	Peflin OS=Danio rerio GN=pef1 PE=2 SV=1	[more]
PEF1_XENLA	5.0e-22	33.48	Peflin OS=Xenopus laevis GN=pef1 PE=1 SV=1	[more]

BLAST of Cp4.1LG09g05540 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LT26_CUCSA	3.4e-110	82.56	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G164670 PE=4 SV=1	[more]
B9HQ10_POPTR	5.5e-76	61.87	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s16510g PE=4 SV=1	[more]
A0A067F4F6_CITSI	7.4e-73	62.15	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g025714mg PE=4 SV=1	[more]
W9QJD2_9ROSA	4.8e-72	62.60	Putative calcium-binding protein CML48 OS=Morus notabilis GN=L484_022335 PE=4 SV...	[more]
V4UDU3_9ROSI	6.3e-72	61.35	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10029136mg PE=4 SV=1	[more]

BLAST of Cp4.1LG09g05540 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G27480.1	7.2e-51	46.19	Calcium-binding EF-hand family protein	[more]
AT5G04170.1	2.3e-49	46.89	Calcium-binding EF-hand family protein	[more]
AT3G10300.3	4.8e-47	43.80	Calcium-binding EF-hand family protein	[more]

BLAST of Cp4.1LG09g05540 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659071684\|ref\|XP_008461406.1\|	1.8e-112	84.11	PREDICTED: probable calcium-binding protein CML48 [Cucumis melo]	[more]
gi\|449443448\|ref\|XP_004139489.1\|	4.9e-110	82.56	PREDICTED: probable calcium-binding protein CML48 [Cucumis sativus]	[more]
gi\|224104857\|ref\|XP_002313594.1\|	7.9e-76	61.87	hypothetical protein POPTR_0009s16510g [Populus trichocarpa]	[more]
gi\|694430513\|ref\|XP_009342731.1\|	2.3e-75	63.45	PREDICTED: probable calcium-binding protein CML48 [Pyrus x bretschneideri]	[more]
gi\|743791922\|ref\|XP_011043032.1\|	6.7e-75	63.56	PREDICTED: probable calcium-binding protein CML48 [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018247	EF_Hand_1_Ca_BS
IPR011992	EF-hand-dom_pair
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0004198	calcium-dependent cysteine-type endopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG09g05540.1	Cp4.1LG09g05540.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 86..112 score: 2.
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 156..174 score: 0.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 85..113 score: 3.3E-4coord: 152..180 score:
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 157..183 score: 10.775coord: 81..116 score: 1
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 85..247 score: 1.3
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 77..247 score: 2.44
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 94..106 score: -coord: 161..173 scor
None	No IPR available	PANTHER	PTHR10183	CALPAIN	coord: 31..254 score: 3.3E
None	No IPR available	PANTHER	PTHR10183:SF299	SUBFAMILY NOT NAMED	coord: 31..254 score: 3.3E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG09g05540	Cucsa.319880	Cucumber (Gy14) v1	cgycpeB0841
Cp4.1LG09g05540	CmaCh18G006550	Cucurbita maxima (Rimu)	cmacpeB415
Cp4.1LG09g05540	CmoCh18G006670	Cucurbita moschata (Rifu)	cmocpeB374
Cp4.1LG09g05540	Cla001094	Watermelon (97103) v1	cpewmB045
Cp4.1LG09g05540	Csa1G164670	Cucumber (Chinese Long) v2	cpecuB020
Cp4.1LG09g05540	MELO3C004702	Melon (DHL92) v3.5.1	cpemeB028
Cp4.1LG09g05540	ClCG01G012200	Watermelon (Charleston Gray)	cpewcgB029
Cp4.1LG09g05540	CSPI01G14730	Wild cucumber (PI 183967)	cpecpiB017
Cp4.1LG09g05540	Lsi03G001220	Bottle gourd (USVL1VR-Ls)	cpelsiB029
Cp4.1LG09g05540	MELO3C004702.2	Melon (DHL92) v3.6.1	cpemedB033
Cp4.1LG09g05540	CsaV3_1G014860	Cucumber (Chinese Long) v3	cpecucB0024
Cp4.1LG09g05540	Bhi08G000045	Wax gourd	cpewgoB0050
Cp4.1LG09g05540	CsGy1G014610	Cucumber (Gy14) v2	cgybcpeB007
Cp4.1LG09g05540	Carg26271	Silver-seed gourd	carcpeB0000

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG09g05540	Cp4.1LG20g06900	Cucurbita pepo (Zucchini)	cpecpeB048

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG09g05540	Watermelon (Charleston Gray)	cpewcgB034
Cp4.1LG09g05540	Watermelon (97103) v1	cpewmB048
Cp4.1LG09g05540	Watermelon (97103) v1	cpewmB053