Cp4.1LG01g18360 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG01g18360
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPlant invertase/pectin methylesterase inhibitor
LocationCp4.1LG01 : 15626964 .. 15627596 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACCTCAATCGTCTCTTATCCTCTCTCTCCTATTCGCAGCCACTCTCTGTCTCCTACGCCAGGTTCCGGCCGTCGCTGCTGCTGATGCTGGGACCTCCGTCAACGCCACTGCCAATAGTACCGAGTTTATCCGTAGTAGCTGTGTCATTACTCTCTATCCCGACGTCTGCTACGCTTCTCTCTCTCGCTACGCTAACGACATTCAGCAGGATCCAGCCTCGCTCACTCGCATCGCCATTACCATCAGCCTTGCCAACTCCCGTAGAATGGCTTCTTATGTATCCAACCTGTCTCACGTCGGCGACTACGGCGCCGACCACAGAGCCGCCTCCGCGCTTCACGATTGCTTCACGAACTTCGACGACGCCGTCGACGAGATTCGCGGCTCGCTCAACCAGATGCGCCAGATCAACGACGTCGATGTGCCGTCGTTCAGGTTTCAAATGAGCAACGTGCAGACGTGGATGAGCGCTGCGCTTACGGATCAGGAAACATGTACGGACGGATTCGAAGATGTGGCCGATGGACCGATGAAAGCGGACGTATGCGCAAAGGCGGAGAAGGTGAAGAAGCACACCAGCAATGCCCTAGCGCTAGTGAATAGCTTCGTCCAGAAAAGGATTCCGTGA

mRNA sequence

ATGAAACCTCAATCGTCTCTTATCCTCTCTCTCCTATTCGCAGCCACTCTCTGTCTCCTACGCCAGGTTCCGGCCGTCGCTGCTGCTGATGCTGGGACCTCCGTCAACGCCACTGCCAATAGTACCGAGTTTATCCGTAGTAGCTGTGTCATTACTCTCTATCCCGACGTCTGCTACGCTTCTCTCTCTCGCTACGCTAACGACATTCAGCAGGATCCAGCCTCGCTCACTCGCATCGCCATTACCATCAGCCTTGCCAACTCCCGTAGAATGGCTTCTTATGTATCCAACCTGTCTCACGTCGGCGACTACGGCGCCGACCACAGAGCCGCCTCCGCGCTTCACGATTGCTTCACGAACTTCGACGACGCCGTCGACGAGATTCGCGGCTCGCTCAACCAGATGCGCCAGATCAACGACGTCGATGTGCCGTCGTTCAGGTTTCAAATGAGCAACGTGCAGACGTGGATGAGCGCTGCGCTTACGGATCAGGAAACATGTACGGACGGATTCGAAGATGTGGCCGATGGACCGATGAAAGCGGACGTATGCGCAAAGGCGGAGAAGGTGAAGAAGCACACCAGCAATGCCCTAGCGCTAGTGAATAGCTTCGTCCAGAAAAGGATTCCGTGA

Coding sequence (CDS)

ATGAAACCTCAATCGTCTCTTATCCTCTCTCTCCTATTCGCAGCCACTCTCTGTCTCCTACGCCAGGTTCCGGCCGTCGCTGCTGCTGATGCTGGGACCTCCGTCAACGCCACTGCCAATAGTACCGAGTTTATCCGTAGTAGCTGTGTCATTACTCTCTATCCCGACGTCTGCTACGCTTCTCTCTCTCGCTACGCTAACGACATTCAGCAGGATCCAGCCTCGCTCACTCGCATCGCCATTACCATCAGCCTTGCCAACTCCCGTAGAATGGCTTCTTATGTATCCAACCTGTCTCACGTCGGCGACTACGGCGCCGACCACAGAGCCGCCTCCGCGCTTCACGATTGCTTCACGAACTTCGACGACGCCGTCGACGAGATTCGCGGCTCGCTCAACCAGATGCGCCAGATCAACGACGTCGATGTGCCGTCGTTCAGGTTTCAAATGAGCAACGTGCAGACGTGGATGAGCGCTGCGCTTACGGATCAGGAAACATGTACGGACGGATTCGAAGATGTGGCCGATGGACCGATGAAAGCGGACGTATGCGCAAAGGCGGAGAAGGTGAAGAAGCACACCAGCAATGCCCTAGCGCTAGTGAATAGCTTCGTCCAGAAAAGGATTCCGTGA

Protein sequence

MKPQSSLILSLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKADVCAKAEKVKKHTSNALALVNSFVQKRIP
BLAST of Cp4.1LG01g18360 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.0e-28
Identity = 78/199 (39.20%), Postives = 116/199 (58.29%), Query Frame = 1

Query: 10  SLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCYASLSRYANDI 69
           +L+F+A L +L      AAA A       A + +FI++SC +T YP VC  SLS YA  I
Sbjct: 7   TLVFSALLVIL------AAASA-------APANQFIKTSCTLTTYPAVCEQSLSAYAKTI 66

Query: 70  QQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIR 129
           Q +P  L   A+ +SL  +++  +++  L+     G   R  +A+HDC    +D++D + 
Sbjct: 67  QNNPQELASTALQVSLTRTQQAQTFMKRLNKFK--GLKARQYAAIHDCLEEVEDSLDRVS 126

Query: 130 GSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVA-DGPMKADVCAKAE 189
            S ++M+ ++      F F+MSNV+TW+SAALTD+ TC DGF     DG +K  V A+  
Sbjct: 127 RSCDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVV 186

Query: 190 KVKKHTSNALALVNSFVQK 208
            V + TSNALALVN+F  K
Sbjct: 187 AVARVTSNALALVNNFAAK 190

BLAST of Cp4.1LG01g18360 vs. Swiss-Prot
Match: PME26_ARATH (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana GN=PME26 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 5.4e-14
Identity = 46/167 (27.54%), Postives = 86/167 (51.50%), Query Frame = 1

Query: 45  IRSSCVITLYPDVCYASLSRYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDY 104
           +R+ C +T YP  C +S+S+  +    DP +L R+++ + +     +A     L+   D 
Sbjct: 271 LRNVCSVTRYPASCVSSISKLPSSNTTDPEALFRLSLQVVINELNSIAGLPKKLAEETD- 330

Query: 105 GADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQ 164
             D R  S+L  C   F+DA+D +  +++ M ++ D         +  +QTW+SAA+TD 
Sbjct: 331 --DERLKSSLSVCGDVFNDAIDIVNDTISTMEEVGDGKKILKSSTIDEIQTWLSAAVTDH 390

Query: 165 ETCTDGFEDV-------ADGPMKADVCAKAEKVKKHTSNALALVNSF 205
           +TC D  +++       A+ P+   + +     +K TSN+LA++  F
Sbjct: 391 DTCLDALDELSQNKTEYANSPISLKLKSAMVNSRKFTSNSLAIIAKF 434

BLAST of Cp4.1LG01g18360 vs. Swiss-Prot
Match: PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.1e-10
Identity = 46/163 (28.22%), Postives = 82/163 (50.31%), Query Frame = 1

Query: 42  TEFIRSSCVITLYPDVCYASLSRYANDIQQDPAS-LTRIAITISLANSRRMASYVSNLSH 101
           ++ I  +C +T +P++C  SL  +   +    +  L  + + ++L +        ++LS 
Sbjct: 84  SQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSF 143

Query: 102 VGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAA 161
           V       RA SA   C    DD+VD +  +L+         V S   +  +V TW+SAA
Sbjct: 144 VD---MPPRARSAYDSCVELLDDSVDALSRALSS--------VVSSSAKPQDVTTWLSAA 203

Query: 162 LTDQETCTDGFEDVADGPMKADVCAKAEKVKKHTSNALALVNS 204
           LT+ +TCT+GF+ V DG +K  + A  + + +  SN LA+ ++
Sbjct: 204 LTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSA 235

BLAST of Cp4.1LG01g18360 vs. Swiss-Prot
Match: PME61_ARATH (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=PME61 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.1e-10
Identity = 50/178 (28.09%), Postives = 84/178 (47.19%), Query Frame = 1

Query: 26  VAAADAG-TSVNATANSTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRIAITIS 85
           + A D+G T    T   T+ I  +C  +LYP++C  +L  +   +  D   L  I+   +
Sbjct: 55  IRAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNAT 114

Query: 86  LANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDVDVP 145
           L    +     S +++        R  SA   C    DD+VD +       R ++ V V 
Sbjct: 115 LQKFSKALYTSSTITYTQ---MPPRVRSAYDSCLELLDDSVDALT------RALSSVVVV 174

Query: 146 SFRFQMSNVQTWMSAALTDQETCTDGFEDV--ADGPMKADVCAKAEKVKKHTSNALAL 201
           S     S+V TW+S+A+T+ +TCTDGF+++    G +K  V    + + +  SN LA+
Sbjct: 175 SGDESHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAI 223

BLAST of Cp4.1LG01g18360 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.0e-09
Identity = 54/201 (26.87%), Postives = 96/201 (47.76%), Query Frame = 1

Query: 12  LFAATLCLLRQVPAVAAADAGTSVNATANSTE--------FIRSSCVITLYPDVCYASLS 71
           LF A    L  V AV    AG  VN+  NS +         ++SSC  T YPD+C+++++
Sbjct: 30  LFLALFATLLVVAAVIGIVAG--VNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIA 89

Query: 72  RYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDD 131
                 ++  +    I +++++  +    +Y      +       R   ALHDC    D+
Sbjct: 90  AVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETIDE 149

Query: 132 AVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGF-EDVADGPMKAD 191
            +DE+  ++  + +  +    S      +++T MSAA+T+Q TC DGF  D A+  ++  
Sbjct: 150 TLDELHKAVEDLEEYPNKK--SLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDA 209

Query: 192 VCAKAEKVKKHTSNALALVNS 204
           +      V+K  SNALA++ +
Sbjct: 210 LSDGQVHVEKMCSNALAMIKN 226

BLAST of Cp4.1LG01g18360 vs. TrEMBL
Match: A0A0A0KPD9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G567800 PE=4 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 7.7e-92
Identity = 183/210 (87.14%), Postives = 187/210 (89.05%), Query Frame = 1

Query: 1   MKPQSSLILSLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCYA 60
           MKPQ  LILSLLFAATL  LR  P  A  D   S NATAN  EFIR+SC ITLYPDVCY 
Sbjct: 1   MKPQRPLILSLLFAATLFYLR--PVSADEDTPNSPNATANCMEFIRTSCGITLYPDVCYT 60

Query: 61  SLSRYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTN 120
           SLSRYANDIQQDPASLTRIAITISLANSRRMA+YVSNLSHVGD GAD RAASALHDCFTN
Sbjct: 61  SLSRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHVGDNGADRRAASALHDCFTN 120

Query: 121 FDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMK 180
           FDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMK
Sbjct: 121 FDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMK 180

Query: 181 ADVCAKAEKVKKHTSNALALVNSFVQKRIP 211
            DVCAKAEKVKKHTSNALALVNSFV+K+IP
Sbjct: 181 EDVCAKAEKVKKHTSNALALVNSFVEKKIP 208

BLAST of Cp4.1LG01g18360 vs. TrEMBL
Match: W9RGV6_9ROSA (21 kDa protein OS=Morus notabilis GN=L484_011998 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 7.5e-63
Identity = 128/206 (62.14%), Postives = 159/206 (77.18%), Query Frame = 1

Query: 10  SLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCYASLSRYANDI 69
           +LLF     +L+ +PAV  AD G+S N T    +FIR+SCV TLYPDVC+ +LSRYA  +
Sbjct: 15  TLLFLIIFSVLKPIPAVGYADPGSSTNGT----DFIRTSCVTTLYPDVCFTALSRYAAAV 74

Query: 70  QQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIR 129
           Q++P  L R+AI++SLA  + +A+YVSN+S   DYGA+ RAA+ALHDCF+NF DAVDEIR
Sbjct: 75  QRNPEQLARVAISVSLAKVKHVAAYVSNVSRQADYGANPRAAAALHDCFSNFGDAVDEIR 134

Query: 130 GSLNQMRQINDVDV-----PSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKADVC 189
           GSL QMR +N          SFRFQMSNVQTWMSAALTDQETCTDGF+DVADGP+K +VC
Sbjct: 135 GSLRQMRHLNVGGTGAGGGESFRFQMSNVQTWMSAALTDQETCTDGFQDVADGPLKKEVC 194

Query: 190 AKAEKVKKHTSNALALVNSFVQKRIP 211
            +A +VKKHTSNALALVNS+ +K +P
Sbjct: 195 DRATEVKKHTSNALALVNSYAEKSVP 216

BLAST of Cp4.1LG01g18360 vs. TrEMBL
Match: A0A061E7V9_THECC (Plant invertase/pectin methylesterase inhibitor superfamily protein (Fragment) OS=Theobroma cacao GN=TCM_010984 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 3.2e-61
Identity = 133/208 (63.94%), Postives = 156/208 (75.00%), Query Frame = 1

Query: 1   MKPQ-SSLILSLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCY 60
           MKP  S L L+ LF     +L  +  V++ D  +S  AT N+T+FIR+SC  TLYPD+CY
Sbjct: 61  MKPLLSHLFLTFLFF----ILHPIVTVSSPDNDSSTTAT-NATDFIRTSCSATLYPDLCY 120

Query: 61  ASLSRYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT 120
            SLS YAN IQQDPA L R AI +SL+ +R MA YVSNLS   DYGAD RA++ALHDCF+
Sbjct: 121 TSLSGYANAIQQDPARLARTAIGVSLSKTRHMAVYVSNLSREADYGADPRASAALHDCFS 180

Query: 121 NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180
           N  DAVDEIRGSL QMRQ+      SFRFQM NVQTWMSAALTD++TCTDGFEDVA+GPM
Sbjct: 181 NMGDAVDEIRGSLKQMRQLVAPGSESFRFQMGNVQTWMSAALTDEDTCTDGFEDVAEGPM 240

Query: 181 KADVCAKAEKVKKHTSNALALVNSFVQK 208
           K DVC +  KVKK TSNALALVNS+ +K
Sbjct: 241 KTDVCQRTGKVKKFTSNALALVNSYAEK 263

BLAST of Cp4.1LG01g18360 vs. TrEMBL
Match: A0A0D2Q813_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G175900 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 9.2e-61
Identity = 126/205 (61.46%), Postives = 152/205 (74.15%), Query Frame = 1

Query: 3   PQSSLILSLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCYASL 62
           P S L L+ LF      L  +P + +A+   +    +N+T+FIR SC  TLYPD+C+ASL
Sbjct: 6   PLSPLFLTFLFFH----LHPIPTLCSANYDANTTTPSNATDFIRISCSATLYPDLCFASL 65

Query: 63  SRYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFD 122
           S YAN IQQDPA L R AI +SL+ +R MA++VSNLS   DYGAD RA+SALHDCF+N  
Sbjct: 66  SGYANAIQQDPARLARTAIGVSLSRARHMAAFVSNLSREADYGADQRASSALHDCFSNIG 125

Query: 123 DAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKAD 182
           DAVDEIRGSLN+MR++ +    SFRFQM NVQTWMSAALTD+ETCTDGFEDV DGPMK  
Sbjct: 126 DAVDEIRGSLNEMRRLVNPGSESFRFQMGNVQTWMSAALTDEETCTDGFEDVGDGPMKTA 185

Query: 183 VCAKAEKVKKHTSNALALVNSFVQK 208
           VC +A  VKK TSNALAL NS+ +K
Sbjct: 186 VCERAANVKKFTSNALALANSYAEK 206

BLAST of Cp4.1LG01g18360 vs. TrEMBL
Match: A0A0D2NDN9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G160200 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 3.0e-59
Identity = 119/178 (66.85%), Postives = 138/178 (77.53%), Query Frame = 1

Query: 30  DAGTSVNATANSTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRIAITISLANSR 89
           D   +     NST+FIR+SC  TLYPD+CY SL  YAN IQQDPA L R AI++SL+ +R
Sbjct: 29  DTSATTANVINSTDFIRTSCYATLYPDICYTSLYGYANAIQQDPARLARAAISVSLSKAR 88

Query: 90  RMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDVDVPSFRFQ 149
            MA YVSNLS   DYGAD RA++ALHDCF+N  DAVDEIRGSL QM+++      SFRFQ
Sbjct: 89  NMAVYVSNLSREADYGADPRASAALHDCFSNMGDAVDEIRGSLKQMQRVVAPGSESFRFQ 148

Query: 150 MSNVQTWMSAALTDQETCTDGFEDVADGPMKADVCAKAEKVKKHTSNALALVNSFVQK 208
           M NVQTWMSAALTD+ETCTDGFEDVA GPMK +VC +A KVKK TSNALALVNS+ +K
Sbjct: 149 MGNVQTWMSAALTDEETCTDGFEDVAGGPMKTEVCKRAAKVKKFTSNALALVNSYAEK 206

BLAST of Cp4.1LG01g18360 vs. TAIR10
Match: AT1G23205.1 (AT1G23205.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 184.5 bits (467), Expect = 6.7e-47
Identity = 93/182 (51.10%), Postives = 136/182 (74.73%), Query Frame = 1

Query: 34  SVNATANSTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRIAITISLANSRRMAS 93
           +V++ ++ ++FIR+SC  TLYPD+C++SLS +++ +  DPA L R AI+++L  +  +AS
Sbjct: 23  TVHSQSDDSDFIRTSCNTTLYPDLCFSSLSSFSSSVHNDPALLARAAISVTLTKTLDLAS 82

Query: 94  YVSNLSHV----GDYGADHRAASAL-HDCFTNFDDAVDEIRGSLNQMRQ-INDVDVPSFR 153
           Y++N++ +     + GA H  A+A+ HDCF N  DAV+E++GS+ QMR+ ++   + SFR
Sbjct: 83  YLANITTLQPESNEDGAHHPTAAAVFHDCFDNLKDAVEEMKGSMKQMRELVSTGSLESFR 142

Query: 154 FQMSNVQTWMSAALTDQETCTDGFEDVADGPMKADVCAKAEKVKKHTSNALALVNSFVQK 210
           FQMSNVQTW+SAALTD+ETCTDGF+D+ D P K D+CA+ + VKK TSNALALVN  V K
Sbjct: 143 FQMSNVQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKMTSNALALVNRCVDK 202

BLAST of Cp4.1LG01g18360 vs. TAIR10
Match: AT2G01610.1 (AT2G01610.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 172.2 bits (435), Expect = 3.5e-43
Identity = 103/219 (47.03%), Postives = 138/219 (63.01%), Query Frame = 1

Query: 4   QSSLILSLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCYASLS 63
           QS     L F   L +   + AV       +   T N  +FIR+SC  TLYPDVC+ SLS
Sbjct: 6   QSHTTTFLFFTTFLLIFGSISAVRLLPRPNTT--TTNDLDFIRTSCNATLYPDVCFTSLS 65

Query: 64  RYANDIQQDPASLTRIAITISLANSRRMASYVSNLSH-VGDYGAD-HRAASA-LHDCFTN 123
            YA+ +Q  PA L ++AI +SL+ ++  A+++S LS     Y  D H+ ASA + DC +N
Sbjct: 66  GYASAVQDSPARLAKLAIGVSLSQAKSTAAFLSKLSRSAAKYSGDGHQTASAVIRDCVSN 125

Query: 124 FDDAVDEIRGSLNQMRQIND--------VDVPSFRFQMSNVQTWMSAALTDQETCTDGFE 183
            +DAVDE+RGSL Q+R +N           V +FRFQMSNVQTWMSAALTD++TCTDGFE
Sbjct: 126 VEDAVDEMRGSLRQLRDMNGRGGGTAARRSVETFRFQMSNVQTWMSAALTDEDTCTDGFE 185

Query: 184 DVADGPM-KADVCAKAEKVKKHTSNALALVNSFVQKRIP 211
           D+ +G + K  VC + E+VK+ TSNALALVN++     P
Sbjct: 186 DMDEGGLIKTTVCDRLEEVKRLTSNALALVNTYANNGAP 222

BLAST of Cp4.1LG01g18360 vs. TAIR10
Match: AT1G14890.1 (AT1G14890.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 171.0 bits (432), Expect = 7.7e-43
Identity = 89/170 (52.35%), Postives = 122/170 (71.76%), Query Frame = 1

Query: 38  TANSTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRIAITISLANSRRMASYVSN 97
           T +  +FIR+SC  TLYPDVCY SL+ YA+ +Q +PA L ++AI +SL+ ++  A+Y+S 
Sbjct: 52  TTDDLDFIRTSCNTTLYPDVCYTSLAGYASAVQDNPARLAKLAIGVSLSRAKYTAAYLSK 111

Query: 98  LSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQIND--VDVPSFRFQMSNVQT 157
           LS          A++A+HDC +N  DAVD++RGSL Q+R++N      P+FRFQMSNVQT
Sbjct: 112 LSR-------RAASAAVHDCVSNVGDAVDQMRGSLRQLREMNHRRPGDPAFRFQMSNVQT 171

Query: 158 WMSAALTDQETCTDGF-EDVADGPMKADVCAKAEKVKKHTSNALALVNSF 205
           WMSAALTD+ETCTDG  E++ DG  K  +C +   VK+ TSNALALVN++
Sbjct: 172 WMSAALTDEETCTDGVTEEMEDGETKTAICDRVADVKRFTSNALALVNTY 214

BLAST of Cp4.1LG01g18360 vs. TAIR10
Match: AT1G70720.1 (AT1G70720.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 164.1 bits (414), Expect = 9.4e-41
Identity = 87/174 (50.00%), Postives = 119/174 (68.39%), Query Frame = 1

Query: 39  ANSTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRIAITISLANSRRMASYVSNL 98
           +N ++FIR +C  TLYPD+C+++LS +AN IQ D   L R+AI+++L N+  + SY+ N 
Sbjct: 31  SNGSDFIRLACNTTLYPDLCFSTLSSFANSIQNDSNRLARVAISLTLHNTLHLLSYLQNA 90

Query: 99  SHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDV--DVPSFRFQMSNVQTW 158
                Y  DH     L DCF N  DAVD +RGS+ QM+++      + SFRFQMSNV+TW
Sbjct: 91  -----YNRDH-PTPVLRDCFENLKDAVDGMRGSMKQMKELVSASGSIESFRFQMSNVKTW 150

Query: 159 MSAALTDQETCTDGFEDV-ADGPMKADVCAKAEKVKKHTSNALALVNSFVQKRI 210
           +SAALTD+ TCTDGF+DV  D  +K DVC++ + VKK TSNALALVN +  + I
Sbjct: 151 LSAALTDEYTCTDGFKDVHEDDSIKDDVCSRVDDVKKLTSNALALVNRYADESI 198

BLAST of Cp4.1LG01g18360 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 124.8 bits (312), Expect = 6.3e-29
Identity = 73/183 (39.89%), Postives = 103/183 (56.28%), Query Frame = 1

Query: 26  VAAADAGTSVNATANSTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRIAITISL 85
           + AA A +   A+  +  FI+SSC  T YP +C  SLS YANDIQ  P  L   AI ++L
Sbjct: 20  LTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTL 79

Query: 86  ANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDV-DVP 145
           + ++    +VS L+ +   G   R   A+ DC    +D VD +  S+ +++      D  
Sbjct: 80  SRAQSTKLFVSRLTRM--KGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQD 139

Query: 146 SFRFQMSNVQTWMSAALTDQETCTDGFED-VADGPMKADVCAKAEKVKKHTSNALALVNS 205
            F + MSN QTW SAALTD+ TC+DGF   V DG +K  V A+   V   TSNAL+L+N+
Sbjct: 140 QFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLINA 199

Query: 206 FVQ 207
           F +
Sbjct: 200 FAK 200

BLAST of Cp4.1LG01g18360 vs. NCBI nr
Match: gi|659072829|ref|XP_008467116.1| (PREDICTED: 21 kDa protein [Cucumis melo])

HSP 1 Score: 347.4 bits (890), Expect = 1.7e-92
Identity = 184/210 (87.62%), Postives = 187/210 (89.05%), Query Frame = 1

Query: 1   MKPQSSLILSLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCYA 60
           MKPQ  LILSLLFAATL  LR  P  A  D   S NATAN  EFIR+SC ITLYPDVCY 
Sbjct: 1   MKPQRPLILSLLFAATLFHLR--PVFANEDTRNSSNATANCIEFIRTSCGITLYPDVCYT 60

Query: 61  SLSRYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTN 120
           SLSRYANDIQQDPASLTRIAITISLANSRRMA+YVSNLSH GDYGAD RAASALHDCFTN
Sbjct: 61  SLSRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHAGDYGADRRAASALHDCFTN 120

Query: 121 FDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMK 180
           FDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMK
Sbjct: 121 FDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMK 180

Query: 181 ADVCAKAEKVKKHTSNALALVNSFVQKRIP 211
            DVCAKAEKVKKHTSNALALVNSFV+KRIP
Sbjct: 181 EDVCAKAEKVKKHTSNALALVNSFVEKRIP 208

BLAST of Cp4.1LG01g18360 vs. NCBI nr
Match: gi|449464804|ref|XP_004150119.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 344.7 bits (883), Expect = 1.1e-91
Identity = 183/210 (87.14%), Postives = 187/210 (89.05%), Query Frame = 1

Query: 1   MKPQSSLILSLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCYA 60
           MKPQ  LILSLLFAATL  LR  P  A  D   S NATAN  EFIR+SC ITLYPDVCY 
Sbjct: 1   MKPQRPLILSLLFAATLFYLR--PVSADEDTPNSPNATANCMEFIRTSCGITLYPDVCYT 60

Query: 61  SLSRYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTN 120
           SLSRYANDIQQDPASLTRIAITISLANSRRMA+YVSNLSHVGD GAD RAASALHDCFTN
Sbjct: 61  SLSRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHVGDNGADRRAASALHDCFTN 120

Query: 121 FDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMK 180
           FDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMK
Sbjct: 121 FDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMK 180

Query: 181 ADVCAKAEKVKKHTSNALALVNSFVQKRIP 211
            DVCAKAEKVKKHTSNALALVNSFV+K+IP
Sbjct: 181 EDVCAKAEKVKKHTSNALALVNSFVEKKIP 208

BLAST of Cp4.1LG01g18360 vs. NCBI nr
Match: gi|703119893|ref|XP_010101980.1| (21 kDa protein [Morus notabilis])

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-62
Identity = 128/206 (62.14%), Postives = 159/206 (77.18%), Query Frame = 1

Query: 10  SLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCYASLSRYANDI 69
           +LLF     +L+ +PAV  AD G+S N T    +FIR+SCV TLYPDVC+ +LSRYA  +
Sbjct: 15  TLLFLIIFSVLKPIPAVGYADPGSSTNGT----DFIRTSCVTTLYPDVCFTALSRYAAAV 74

Query: 70  QQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIR 129
           Q++P  L R+AI++SLA  + +A+YVSN+S   DYGA+ RAA+ALHDCF+NF DAVDEIR
Sbjct: 75  QRNPEQLARVAISVSLAKVKHVAAYVSNVSRQADYGANPRAAAALHDCFSNFGDAVDEIR 134

Query: 130 GSLNQMRQINDVDV-----PSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKADVC 189
           GSL QMR +N          SFRFQMSNVQTWMSAALTDQETCTDGF+DVADGP+K +VC
Sbjct: 135 GSLRQMRHLNVGGTGAGGGESFRFQMSNVQTWMSAALTDQETCTDGFQDVADGPLKKEVC 194

Query: 190 AKAEKVKKHTSNALALVNSFVQKRIP 211
            +A +VKKHTSNALALVNS+ +K +P
Sbjct: 195 DRATEVKKHTSNALALVNSYAEKSVP 216

BLAST of Cp4.1LG01g18360 vs. NCBI nr
Match: gi|590696637|ref|XP_007045219.1| (Plant invertase/pectin methylesterase inhibitor superfamily protein, partial [Theobroma cacao])

HSP 1 Score: 243.0 bits (619), Expect = 4.5e-61
Identity = 133/208 (63.94%), Postives = 156/208 (75.00%), Query Frame = 1

Query: 1   MKPQ-SSLILSLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCY 60
           MKP  S L L+ LF     +L  +  V++ D  +S  AT N+T+FIR+SC  TLYPD+CY
Sbjct: 61  MKPLLSHLFLTFLFF----ILHPIVTVSSPDNDSSTTAT-NATDFIRTSCSATLYPDLCY 120

Query: 61  ASLSRYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT 120
            SLS YAN IQQDPA L R AI +SL+ +R MA YVSNLS   DYGAD RA++ALHDCF+
Sbjct: 121 TSLSGYANAIQQDPARLARTAIGVSLSKTRHMAVYVSNLSREADYGADPRASAALHDCFS 180

Query: 121 NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180
           N  DAVDEIRGSL QMRQ+      SFRFQM NVQTWMSAALTD++TCTDGFEDVA+GPM
Sbjct: 181 NMGDAVDEIRGSLKQMRQLVAPGSESFRFQMGNVQTWMSAALTDEDTCTDGFEDVAEGPM 240

Query: 181 KADVCAKAEKVKKHTSNALALVNSFVQK 208
           K DVC +  KVKK TSNALALVNS+ +K
Sbjct: 241 KTDVCQRTGKVKKFTSNALALVNSYAEK 263

BLAST of Cp4.1LG01g18360 vs. NCBI nr
Match: gi|823134930|ref|XP_012467250.1| (PREDICTED: 21 kDa protein [Gossypium raimondii])

HSP 1 Score: 241.5 bits (615), Expect = 1.3e-60
Identity = 126/205 (61.46%), Postives = 152/205 (74.15%), Query Frame = 1

Query: 3   PQSSLILSLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCYASL 62
           P S L L+ LF      L  +P + +A+   +    +N+T+FIR SC  TLYPD+C+ASL
Sbjct: 6   PLSPLFLTFLFFH----LHPIPTLCSANYDANTTTPSNATDFIRISCSATLYPDLCFASL 65

Query: 63  SRYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFD 122
           S YAN IQQDPA L R AI +SL+ +R MA++VSNLS   DYGAD RA+SALHDCF+N  
Sbjct: 66  SGYANAIQQDPARLARTAIGVSLSRARHMAAFVSNLSREADYGADQRASSALHDCFSNIG 125

Query: 123 DAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKAD 182
           DAVDEIRGSLN+MR++ +    SFRFQM NVQTWMSAALTD+ETCTDGFEDV DGPMK  
Sbjct: 126 DAVDEIRGSLNEMRRLVNPGSESFRFQMGNVQTWMSAALTDEETCTDGFEDVGDGPMKTA 185

Query: 183 VCAKAEKVKKHTSNALALVNSFVQK 208
           VC +A  VKK TSNALAL NS+ +K
Sbjct: 186 VCERAANVKKFTSNALALANSYAEK 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
21KD_DAUCA3.0e-2839.2021 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME26_ARATH5.4e-1427.54Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana GN=P... [more]
PME34_ARATH2.1e-1028.22Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=P... [more]
PME61_ARATH2.1e-1028.09Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=P... [more]
PME1_CITSI4.0e-0926.87Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KPD9_CUCSA7.7e-9287.14Uncharacterized protein OS=Cucumis sativus GN=Csa_5G567800 PE=4 SV=1[more]
W9RGV6_9ROSA7.5e-6362.1421 kDa protein OS=Morus notabilis GN=L484_011998 PE=4 SV=1[more]
A0A061E7V9_THECC3.2e-6163.94Plant invertase/pectin methylesterase inhibitor superfamily protein (Fragment) O... [more]
A0A0D2Q813_GOSRA9.2e-6161.46Uncharacterized protein OS=Gossypium raimondii GN=B456_002G175900 PE=4 SV=1[more]
A0A0D2NDN9_GOSRA3.0e-5966.85Uncharacterized protein OS=Gossypium raimondii GN=B456_005G160200 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G23205.16.7e-4751.10 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT2G01610.13.5e-4347.03 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G14890.17.7e-4352.35 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G70720.19.4e-4150.00 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.16.3e-2939.89 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659072829|ref|XP_008467116.1|1.7e-9287.62PREDICTED: 21 kDa protein [Cucumis melo][more]
gi|449464804|ref|XP_004150119.1|1.1e-9187.14PREDICTED: 21 kDa protein-like [Cucumis sativus][more]
gi|703119893|ref|XP_010101980.1|1.1e-6262.1421 kDa protein [Morus notabilis][more]
gi|590696637|ref|XP_007045219.1|4.5e-6163.94Plant invertase/pectin methylesterase inhibitor superfamily protein, partial [Th... [more]
gi|823134930|ref|XP_012467250.1|1.3e-6061.46PREDICTED: 21 kDa protein [Gossypium raimondii][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g18360.1Cp4.1LG01g18360.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 41..204
score: 3.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 44..200
score: 1.0
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 40..200
score: 3.9
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 16..202
score: 8.0
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 43..204
score: 1.44
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 1..210
score: 1.2
NoneNo IPR availablePANTHERPTHR31080:SF10PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR DOMAIN-CONTAINING PROTEINcoord: 1..210
score: 1.2

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG01g18360Cp4.1LG13g03530Cucurbita pepo (Zucchini)cpecpeB200