CmaCh15G009690 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh15G009690
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein
LocationCma_Chr15 : 5413200 .. 5413826 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACCCCAACCGTCTTCTATCGTCTCTCTCCTGTTCGCAGCCGGTCTCTTCCTCCTCCGCCTTGTTCCATCCGCTGCCGATGCCGGCACCTTTCCCAATGCCACCGCCGATTGTTCTGAGTTCATCCGCACTAGCTGCGTCATTACTCTCTATCCCGACGTCTGCTACACTTCGCTCTCTCGCTACGCCAATGCCATTCAGCAGGACCCGGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACGCCCGTAGAATGGCTGCTTATGTCTCCAACCTGTCTCACGTAGCTGACTACGGCGCCGACCACAGAGTCTCCTCCGCGCTTCACGACTGCTTCACGAACTTCGACGATGCCGTGGACGAGATTCGCGGCTCACTCAAGCAGATGCGCCAGATCAACGATGTCGACGCGCCGTCGTTTAGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCGGCGCTAACGGATCAAGAAACGTGTACGGACGGATTCGATGATGTGATGGACGGACCGATTAAGAAGGACGTGTGCGCAAAGGCGGAGAAGGTGAAGAAGCACACCAGTAATGCCTTGGCGCTGGTGAACAGCTTCGTGGAGAAAAGGATTCCGTGA

mRNA sequence

ATGAAACCCCAACCGTCTTCTATCGTCTCTCTCCTGTTCGCAGCCGGTCTCTTCCTCCTCCGCCTTGTTCCATCCGCTGCCGATGCCGGCACCTTTCCCAATGCCACCGCCGATTGTTCTGAGTTCATCCGCACTAGCTGCGTCATTACTCTCTATCCCGACGTCTGCTACACTTCGCTCTCTCGCTACGCCAATGCCATTCAGCAGGACCCGGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACGCCCGTAGAATGGCTGCTTATGTCTCCAACCTGTCTCACGTAGCTGACTACGGCGCCGACCACAGAGTCTCCTCCGCGCTTCACGACTGCTTCACGAACTTCGACGATGCCGTGGACGAGATTCGCGGCTCACTCAAGCAGATGCGCCAGATCAACGATGTCGACGCGCCGTCGTTTAGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCGGCGCTAACGGATCAAGAAACGTGTACGGACGGATTCGATGATGTGATGGACGGACCGATTAAGAAGGACGTGTGCGCAAAGGCGGAGAAGGTGAAGAAGCACACCAGTAATGCCTTGGCGCTGGTGAACAGCTTCGTGGAGAAAAGGATTCCGTGA

Coding sequence (CDS)

ATGAAACCCCAACCGTCTTCTATCGTCTCTCTCCTGTTCGCAGCCGGTCTCTTCCTCCTCCGCCTTGTTCCATCCGCTGCCGATGCCGGCACCTTTCCCAATGCCACCGCCGATTGTTCTGAGTTCATCCGCACTAGCTGCGTCATTACTCTCTATCCCGACGTCTGCTACACTTCGCTCTCTCGCTACGCCAATGCCATTCAGCAGGACCCGGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACGCCCGTAGAATGGCTGCTTATGTCTCCAACCTGTCTCACGTAGCTGACTACGGCGCCGACCACAGAGTCTCCTCCGCGCTTCACGACTGCTTCACGAACTTCGACGATGCCGTGGACGAGATTCGCGGCTCACTCAAGCAGATGCGCCAGATCAACGATGTCGACGCGCCGTCGTTTAGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCGGCGCTAACGGATCAAGAAACGTGTACGGACGGATTCGATGATGTGATGGACGGACCGATTAAGAAGGACGTGTGCGCAAAGGCGGAGAAGGTGAAGAAGCACACCAGTAATGCCTTGGCGCTGGTGAACAGCTTCGTGGAGAAAAGGATTCCGTGA

Protein sequence

MKPQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRIP
Homology
[Close]
BLAST of CmaCh15G009690 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 4.1e-30
Identity = 80/197 (40.61%), Postives = 115/197 (58.38%), Query Frame = 1 

Query: 10  SLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQ 69
           +L+F+A L +L    +AA A       A  ++FI+TSC +T YP VC  SLS YA  IQ 
Sbjct: 7   TLVFSALLVIL----AAASA-------APANQFIKTSCTLTTYPAVCEQSLSAYAKTIQN 66

Query: 70  DPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFDDAVDEIRGS 129
           +P  L   A+ +SL   ++   ++  L+     G   R  +A+HDC    +D++D +  S
Sbjct: 67  NPQELASTALQVSLTRTQQAQTFMKRLNKFK--GLKARQYAAIHDCLEEVEDSLDRVSRS 126

Query: 130 LKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF-DDVMDGPIKKDVCAKAEKV 189
             +M+ ++      F F+MSNV+TW+SAALTD+ TC DGF    MDG IK+ V A+   V
Sbjct: 127 CDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAV 186

Query: 190 KKHTSNALALVNSFVEK 206
            + TSNALALVN+F  K
Sbjct: 187 ARVTSNALALVNNFAAK 190

[Close]
BLAST of CmaCh15G009690 vs. Swiss-Prot
Match: PME26_ARATH (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana GN=PME26 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 47/167 (28.14%), Postives = 85/167 (50.90%), Query Frame = 1 

Query: 43  IRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADY 102
           +R  C +T YP  C +S+S+  ++   DP +L R+++ + +     +A     L+   D 
Sbjct: 271 LRNVCSVTRYPASCVSSISKLPSSNTTDPEALFRLSLQVVINELNSIAGLPKKLAEETD- 330

Query: 103 GADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQ 162
             D R+ S+L  C   F+DA+D +  ++  M ++ D         +  +QTW+SAA+TD 
Sbjct: 331 --DERLKSSLSVCGDVFNDAIDIVNDTISTMEEVGDGKKILKSSTIDEIQTWLSAAVTDH 390

Query: 163 ETCTDGFDDV-------MDGPIKKDVCAKAEKVKKHTSNALALVNSF 203
           +TC D  D++        + PI   + +     +K TSN+LA++  F
Sbjct: 391 DTCLDALDELSQNKTEYANSPISLKLKSAMVNSRKFTSNSLAIIAKF 434

[Close]
BLAST of CmaCh15G009690 vs. Swiss-Prot
Match: PME61_ARATH (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=PME61 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 8.6e-12
Identity = 51/176 (28.98%), Postives = 88/176 (50.00%), Query Frame = 1 

Query: 26  AADAG-TFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLA 85
           A D+G T P  T   ++ I  +C  +LYP++C  +L  +  ++  D   L  ++   +L 
Sbjct: 57  AVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQ 116

Query: 86  NARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDAPSF 145
              + A Y S  S +       RV SA   C    DD+VD +  +L  +  ++  ++ S 
Sbjct: 117 KFSK-ALYTS--STITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS- 176

Query: 146 RFQMSNVQTWMSAALTDQETCTDGFDDV--MDGPIKKDVCAKAEKVKKHTSNALAL 199
                +V TW+S+A+T+ +TCTDGFD++    G +K  V    + + +  SN LA+
Sbjct: 177 -----DVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAI 223

[Close]
BLAST of CmaCh15G009690 vs. Swiss-Prot
Match: PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 4.3e-11
Identity = 58/199 (29.15%), Postives = 97/199 (48.74%), Query Frame = 1 

Query: 9   VSLLFAAGLFL-----LRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRY 68
           +SL+ AA +F      L+L  S       P      S+ I  +C +T +P++C  SL  +
Sbjct: 54  ISLILAAAIFAGVRSRLKLNQSV------PGLARKPSQAISKACELTRFPELCVDSLMDF 113

Query: 69  ANAIQQDPAS-LTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFDDA 128
             ++    +  L  V + ++L +        ++LS V       R  SA   C    DD+
Sbjct: 114 PGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFV---DMPPRARSAYDSCVELLDDS 173

Query: 129 VDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKKDVC 188
           VD +       R ++ V + S + Q  +V TW+SAALT+ +TCT+GFD V DG +K  + 
Sbjct: 174 VDALS------RALSSVVSSSAKPQ--DVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMT 233

Query: 189 AKAEKVKKHTSNALALVNS 202
           A  + + +  SN LA+ ++
Sbjct: 234 AALQNLSELVSNCLAIFSA 235

[Close]
BLAST of CmaCh15G009690 vs. Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.8e-10
Identity = 44/168 (26.19%), Postives = 90/168 (53.57%), Query Frame = 1 

Query: 43  IRTSCVITLYPDVCYTSLSRYANAIQQ--DPASLTRVAITISLANARRMAAYVSNLSHVA 102
           ++++C  TL+P++CY+++   ++  ++      +  +++ I++   RR    V  L    
Sbjct: 66  VKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTR 125

Query: 103 DYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALT 162
             G   R   ALHDC    D+ +DE+  +++ +    +    S +  + +++T +S+A+T
Sbjct: 126 K-GLTPREKVALHDCLETMDETLDELHTAVEDLELYPN--KKSLKEHVEDLKTLISSAIT 185

Query: 163 DQETCTDGFD-DVMDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRI 208
           +QETC DGF  D  D  ++K +    + V+K  SNALA++ +  +  I
Sbjct: 186 NQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDI 230

[Close]
BLAST of CmaCh15G009690 vs. TrEMBL
Match: A0A0A0KPD9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G567800 PE=4 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 3.4e-92
Identity = 177/208 (85.10%), Postives = 187/208 (89.90%), Query Frame = 1 

Query: 1   MKPQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60
           MKPQ   I+SLLFAA LF LR V +  D    PNATA+C EFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQRPLILSLLFAATLFYLRPVSADEDTPNSPNATANCMEFIRTSCGITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFD 120
           SRYAN IQQDPASLTR+AITISLAN+RRMAAYVSNLSHV D GAD R +SALHDCFTNFD
Sbjct: 61  SRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHVGDNGADRRAASALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP+K+D
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKED 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEK+IP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKKIP 208

[Close]
BLAST of CmaCh15G009690 vs. TrEMBL
Match: W9RGV6_9ROSA (21 kDa protein OS=Morus notabilis GN=L484_011998 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 9.7e-63
Identity = 130/206 (63.11%), Postives = 159/206 (77.18%), Query Frame = 1 

Query: 10  SLLFAAGLFLLRLVPSA--ADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAI 69
           +LLF     +L+ +P+   AD G+  N T    +FIRTSCV TLYPDVC+T+LSRYA A+
Sbjct: 15  TLLFLIIFSVLKPIPAVGYADPGSSTNGT----DFIRTSCVTTLYPDVCFTALSRYAAAV 74

Query: 70  QQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFDDAVDEIR 129
           Q++P  L RVAI++SLA  + +AAYVSN+S  ADYGA+ R ++ALHDCF+NF DAVDEIR
Sbjct: 75  QRNPEQLARVAISVSLAKVKHVAAYVSNVSRQADYGANPRAAAALHDCFSNFGDAVDEIR 134

Query: 130 GSLKQMRQIN-----DVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKKDVC 189
           GSL+QMR +N          SFRFQMSNVQTWMSAALTDQETCTDGF DV DGP+KK+VC
Sbjct: 135 GSLRQMRHLNVGGTGAGGGESFRFQMSNVQTWMSAALTDQETCTDGFQDVADGPLKKEVC 194

Query: 190 AKAEKVKKHTSNALALVNSFVEKRIP 209
            +A +VKKHTSNALALVNS+ EK +P
Sbjct: 195 DRATEVKKHTSNALALVNSYAEKSVP 216

[Close]
BLAST of CmaCh15G009690 vs. TrEMBL
Match: A0A061E7V9_THECC (Plant invertase/pectin methylesterase inhibitor superfamily protein (Fragment) OS=Theobroma cacao GN=TCM_010984 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 3.1e-61
Identity = 132/208 (63.46%), Postives = 155/208 (74.52%), Query Frame = 1 

Query: 1   MKPQPSSIVSLLFAAGLFLLR---LVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCY 60
           MKP    ++S LF   LF +    +  S+ D  +   AT + ++FIRTSC  TLYPD+CY
Sbjct: 61  MKP----LLSHLFLTFLFFILHPIVTVSSPDNDSSTTAT-NATDFIRTSCSATLYPDLCY 120

Query: 61  TSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFT 120
           TSLS YANAIQQDPA L R AI +SL+  R MA YVSNLS  ADYGAD R S+ALHDCF+
Sbjct: 121 TSLSGYANAIQQDPARLARTAIGVSLSKTRHMAVYVSNLSREADYGADPRASAALHDCFS 180

Query: 121 NFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPI 180
           N  DAVDEIRGSLKQMRQ+    + SFRFQM NVQTWMSAALTD++TCTDGF+DV +GP+
Sbjct: 181 NMGDAVDEIRGSLKQMRQLVAPGSESFRFQMGNVQTWMSAALTDEDTCTDGFEDVAEGPM 240

Query: 181 KKDVCAKAEKVKKHTSNALALVNSFVEK 206
           K DVC +  KVKK TSNALALVNS+ EK
Sbjct: 241 KTDVCQRTGKVKKFTSNALALVNSYAEK 263

[Close]
BLAST of CmaCh15G009690 vs. TrEMBL
Match: A0A0D2NDN9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G160200 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 2.6e-60
Identity = 119/166 (71.69%), Postives = 137/166 (82.53%), Query Frame = 1 

Query: 40  SEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHV 99
           ++FIRTSC  TLYPD+CYTSL  YANAIQQDPA L R AI++SL+ AR MA YVSNLS  
Sbjct: 41  TDFIRTSCYATLYPDICYTSLYGYANAIQQDPARLARAAISVSLSKARNMAVYVSNLSRE 100

Query: 100 ADYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAAL 159
           ADYGAD R S+ALHDCF+N  DAVDEIRGSLKQM+++    + SFRFQM NVQTWMSAAL
Sbjct: 101 ADYGADPRASAALHDCFSNMGDAVDEIRGSLKQMQRVVAPGSESFRFQMGNVQTWMSAAL 160

Query: 160 TDQETCTDGFDDVMDGPIKKDVCAKAEKVKKHTSNALALVNSFVEK 206
           TD+ETCTDGF+DV  GP+K +VC +A KVKK TSNALALVNS+ EK
Sbjct: 161 TDEETCTDGFEDVAGGPMKTEVCKRAAKVKKFTSNALALVNSYAEK 206

[Close]
BLAST of CmaCh15G009690 vs. TrEMBL
Match: A0A067FMZ7_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g028183mg PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.3e-59
Identity = 123/205 (60.00%), Postives = 155/205 (75.61%), Query Frame = 1 

Query: 3   PQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADC-SEFIRTSCVITLYPDVCYTSLS 62
           P+P  ++S LF+     L  +P+AA A   P+  +   ++FIRTSC  TLYP++CY++LS
Sbjct: 4   PRPLILLSFLFSTFFLQLHAIPAAAPASYPPDTGSGTGTDFIRTSCNSTLYPEICYSTLS 63

Query: 63  RYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFDD 122
           RYA+ IQQDPA L  VAI ++L+ A+RMA YVSN+S  ADYG+D R ++ALHDCF+NF D
Sbjct: 64  RYASTIQQDPAQLASVAIGVTLSKAKRMANYVSNISRQADYGSDQRAAAALHDCFSNFGD 123

Query: 123 AVDEIRGSLKQMRQIND--VDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKK 182
           AV+EI GSLKQMRQI        SFRFQMSNVQTWMSAALTD+ETCTDGF+DV DG +K+
Sbjct: 124 AVEEIYGSLKQMRQIRSAGTSRASFRFQMSNVQTWMSAALTDEETCTDGFEDVADGQMKE 183

Query: 183 DVCAKAEKVKKHTSNALALVNSFVE 205
           +VC + E VKK TSNALALVN + E
Sbjct: 184 EVCDRVEYVKKLTSNALALVNRYAE 208

[Close]
BLAST of CmaCh15G009690 vs. TAIR10
Match: AT1G23205.1 (AT1G23205.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 184.9 bits (468), Expect = 5.1e-47
Identity = 94/180 (52.22%), Postives = 134/180 (74.44%), Query Frame = 1 

Query: 34  NATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYV 93
           ++ +D S+FIRTSC  TLYPD+C++SLS +++++  DPA L R AI+++L     +A+Y+
Sbjct: 25  HSQSDDSDFIRTSCNTTLYPDLCFSSLSSFSSSVHNDPALLARAAISVTLTKTLDLASYL 84

Query: 94  SNLSHVA----DYGADHRVSSAL-HDCFTNFDDAVDEIRGSLKQMRQINDVDA-PSFRFQ 153
           +N++ +     + GA H  ++A+ HDCF N  DAV+E++GS+KQMR++    +  SFRFQ
Sbjct: 85  ANITTLQPESNEDGAHHPTAAAVFHDCFDNLKDAVEEMKGSMKQMRELVSTGSLESFRFQ 144

Query: 154 MSNVQTWMSAALTDQETCTDGFDDVMDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRI 208
           MSNVQTW+SAALTD+ETCTDGF D+ D P K D+CA+ + VKK TSNALALVN  V+K I
Sbjct: 145 MSNVQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKMTSNALALVNRCVDKAI 204

[Close]
BLAST of CmaCh15G009690 vs. TAIR10
Match: AT1G14890.1 (AT1G14890.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 179.9 bits (455), Expect = 1.6e-45
Identity = 102/191 (53.40%), Postives = 132/191 (69.11%), Query Frame = 1 

Query: 17  LFLLRLVPSAADAGTFP--NATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASL 76
           LF+ R +     A  FP    T D  +FIRTSC  TLYPDVCYTSL+ YA+A+Q +PA L
Sbjct: 35  LFICRTI----SAVRFPPEQPTTDDLDFIRTSCNTTLYPDVCYTSLAGYASAVQDNPARL 94

Query: 77  TRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMR 136
            ++AI +SL+ A+  AAY+S LS  A        S+A+HDC +N  DAVD++RGSL+Q+R
Sbjct: 95  AKLAIGVSLSRAKYTAAYLSKLSRRA-------ASAAVHDCVSNVGDAVDQMRGSLRQLR 154

Query: 137 QIND--VDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVM-DGPIKKDVCAKAEKVKKH 196
           ++N      P+FRFQMSNVQTWMSAALTD+ETCTDG  + M DG  K  +C +   VK+ 
Sbjct: 155 EMNHRRPGDPAFRFQMSNVQTWMSAALTDEETCTDGVTEEMEDGETKTAICDRVADVKRF 214

Query: 197 TSNALALVNSF 203
           TSNALALVN++
Sbjct: 215 TSNALALVNTY 214

[Close]
BLAST of CmaCh15G009690 vs. TAIR10
Match: AT2G01610.1 (AT2G01610.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 178.7 bits (452), Expect = 3.7e-45
Identity = 106/211 (50.24%), Postives = 140/211 (66.35%), Query Frame = 1 

Query: 11  LLFAAGLFLLRLVPSAADAGTFPNATADCS-EFIRTSCVITLYPDVCYTSLSRYANAIQQ 70
           LL    +  +RL+P        PN T     +FIRTSC  TLYPDVC+TSLS YA+A+Q 
Sbjct: 19  LLIFGSISAVRLLPR-------PNTTTTNDLDFIRTSCNATLYPDVCFTSLSGYASAVQD 78

Query: 71  DPASLTRVAITISLANARRMAAYVSNLSH-VADYGAD-HRVSSA-LHDCFTNFDDAVDEI 130
            PA L ++AI +SL+ A+  AA++S LS   A Y  D H+ +SA + DC +N +DAVDE+
Sbjct: 79  SPARLAKLAIGVSLSQAKSTAAFLSKLSRSAAKYSGDGHQTASAVIRDCVSNVEDAVDEM 138

Query: 131 RGSLKQMRQIND--------VDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGP-I 190
           RGSL+Q+R +N             +FRFQMSNVQTWMSAALTD++TCTDGF+D+ +G  I
Sbjct: 139 RGSLRQLRDMNGRGGGTAARRSVETFRFQMSNVQTWMSAALTDEDTCTDGFEDMDEGGLI 198

Query: 191 KKDVCAKAEKVKKHTSNALALVNSFVEKRIP 209
           K  VC + E+VK+ TSNALALVN++     P
Sbjct: 199 KTTVCDRLEEVKRLTSNALALVNTYANNGAP 222

[Close]
BLAST of CmaCh15G009690 vs. TAIR10
Match: AT1G70720.1 (AT1G70720.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 164.5 bits (415), Expect = 7.2e-41
Identity = 90/178 (50.56%), Postives = 121/178 (67.98%), Query Frame = 1 

Query: 33  PNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAY 92
           P   ++ S+FIR +C  TLYPD+C+++LS +AN+IQ D   L RVAI+++L N   + +Y
Sbjct: 27  PLLPSNGSDFIRLACNTTLYPDLCFSTLSSFANSIQNDSNRLARVAISLTLHNTLHLLSY 86

Query: 93  VSNLSHVADYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDA--PSFRFQMSN 152
           + N      Y  DH  +  L DCF N  DAVD +RGS+KQM+++        SFRFQMSN
Sbjct: 87  LQNA-----YNRDH-PTPVLRDCFENLKDAVDGMRGSMKQMKELVSASGSIESFRFQMSN 146

Query: 153 VQTWMSAALTDQETCTDGFDDV-MDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRI 208
           V+TW+SAALTD+ TCTDGF DV  D  IK DVC++ + VKK TSNALALVN + ++ I
Sbjct: 147 VKTWLSAALTDEYTCTDGFKDVHEDDSIKDDVCSRVDDVKKLTSNALALVNRYADESI 198

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BLAST of CmaCh15G009690 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 129.0 bits (323), Expect = 3.3e-30
Identity = 77/189 (40.74%), Postives = 107/189 (56.61%), Query Frame = 1 

Query: 18  FLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRV 77
           +LL L  +AA A +   A+     FI++SC  T YP +C  SLS YAN IQ  P  L   
Sbjct: 14  YLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAET 73

Query: 78  AITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQIN 137
           AI ++L+ A+    +VS L+ +   G   R   A+ DC    +D VD +  S+++++   
Sbjct: 74  AIAVTLSRAQSTKLFVSRLTRMK--GLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCG 133

Query: 138 DV-DAPSFRFQMSNVQTWMSAALTDQETCTDGFDD-VMDGPIKKDVCAKAEKVKKHTSNA 197
              D   F + MSN QTW SAALTD+ TC+DGF   VMDG IK  V A+   V   TSNA
Sbjct: 134 SAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNA 193

Query: 198 LALVNSFVE 205
           L+L+N+F +
Sbjct: 194 LSLINAFAK 200

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BLAST of CmaCh15G009690 vs. NCBI nr
Match: gi|449464804|ref|XP_004150119.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 345.9 bits (886), Expect = 4.9e-92
Identity = 177/208 (85.10%), Postives = 187/208 (89.90%), Query Frame = 1 

Query: 1   MKPQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60
           MKPQ   I+SLLFAA LF LR V +  D    PNATA+C EFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQRPLILSLLFAATLFYLRPVSADEDTPNSPNATANCMEFIRTSCGITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFD 120
           SRYAN IQQDPASLTR+AITISLAN+RRMAAYVSNLSHV D GAD R +SALHDCFTNFD
Sbjct: 61  SRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHVGDNGADRRAASALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP+K+D
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKED 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEK+IP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKKIP 208

[Close]
BLAST of CmaCh15G009690 vs. NCBI nr
Match: gi|659072829|ref|XP_008467116.1| (PREDICTED: 21 kDa protein [Cucumis melo])

HSP 1 Score: 343.6 bits (880), Expect = 2.4e-91
Identity = 177/208 (85.10%), Postives = 186/208 (89.42%), Query Frame = 1 

Query: 1   MKPQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60
           MKPQ   I+SLLFAA LF LR V +  D     NATA+C EFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQRPLILSLLFAATLFHLRPVFANEDTRNSSNATANCIEFIRTSCGITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFD 120
           SRYAN IQQDPASLTR+AITISLAN+RRMAAYVSNLSH  DYGAD R +SALHDCFTNFD
Sbjct: 61  SRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHAGDYGADRRAASALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP+K+D
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKED 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]
BLAST of CmaCh15G009690 vs. NCBI nr
Match: gi|703119893|ref|XP_010101980.1| (21 kDa protein [Morus notabilis])

HSP 1 Score: 248.1 bits (632), Expect = 1.4e-62
Identity = 130/206 (63.11%), Postives = 159/206 (77.18%), Query Frame = 1 

Query: 10  SLLFAAGLFLLRLVPSA--ADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAI 69
           +LLF     +L+ +P+   AD G+  N T    +FIRTSCV TLYPDVC+T+LSRYA A+
Sbjct: 15  TLLFLIIFSVLKPIPAVGYADPGSSTNGT----DFIRTSCVTTLYPDVCFTALSRYAAAV 74

Query: 70  QQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFDDAVDEIR 129
           Q++P  L RVAI++SLA  + +AAYVSN+S  ADYGA+ R ++ALHDCF+NF DAVDEIR
Sbjct: 75  QRNPEQLARVAISVSLAKVKHVAAYVSNVSRQADYGANPRAAAALHDCFSNFGDAVDEIR 134

Query: 130 GSLKQMRQIN-----DVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKKDVC 189
           GSL+QMR +N          SFRFQMSNVQTWMSAALTDQETCTDGF DV DGP+KK+VC
Sbjct: 135 GSLRQMRHLNVGGTGAGGGESFRFQMSNVQTWMSAALTDQETCTDGFQDVADGPLKKEVC 194

Query: 190 AKAEKVKKHTSNALALVNSFVEKRIP 209
            +A +VKKHTSNALALVNS+ EK +P
Sbjct: 195 DRATEVKKHTSNALALVNSYAEKSVP 216

[Close]
BLAST of CmaCh15G009690 vs. NCBI nr
Match: gi|590696637|ref|XP_007045219.1| (Plant invertase/pectin methylesterase inhibitor superfamily protein, partial [Theobroma cacao])

HSP 1 Score: 243.0 bits (619), Expect = 4.5e-61
Identity = 132/208 (63.46%), Postives = 155/208 (74.52%), Query Frame = 1 

Query: 1   MKPQPSSIVSLLFAAGLFLLR---LVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCY 60
           MKP    ++S LF   LF +    +  S+ D  +   AT + ++FIRTSC  TLYPD+CY
Sbjct: 61  MKP----LLSHLFLTFLFFILHPIVTVSSPDNDSSTTAT-NATDFIRTSCSATLYPDLCY 120

Query: 61  TSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFT 120
           TSLS YANAIQQDPA L R AI +SL+  R MA YVSNLS  ADYGAD R S+ALHDCF+
Sbjct: 121 TSLSGYANAIQQDPARLARTAIGVSLSKTRHMAVYVSNLSREADYGADPRASAALHDCFS 180

Query: 121 NFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPI 180
           N  DAVDEIRGSLKQMRQ+    + SFRFQM NVQTWMSAALTD++TCTDGF+DV +GP+
Sbjct: 181 NMGDAVDEIRGSLKQMRQLVAPGSESFRFQMGNVQTWMSAALTDEDTCTDGFEDVAEGPM 240

Query: 181 KKDVCAKAEKVKKHTSNALALVNSFVEK 206
           K DVC +  KVKK TSNALALVNS+ EK
Sbjct: 241 KTDVCQRTGKVKKFTSNALALVNSYAEK 263

[Close]
BLAST of CmaCh15G009690 vs. NCBI nr
Match: gi|694320679|ref|XP_009351520.1| (PREDICTED: 21 kDa protein-like [Pyrus x bretschneideri])

HSP 1 Score: 242.3 bits (617), Expect = 7.7e-61
Identity = 125/174 (71.84%), Postives = 141/174 (81.03%), Query Frame = 1 

Query: 40  SEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHV 99
           SEFIRTSC  TLYPD+CYTSLSR+A+A+Q +P  L +VAI +SLA ARR+AAYVSN+S  
Sbjct: 38  SEFIRTSCGTTLYPDLCYTSLSRFASAVQDNPTRLAKVAIGVSLARARRIAAYVSNISRQ 97

Query: 100 ADYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDV-----DAPSFRFQMSNVQTW 159
           ADYGAD R SSALHDCF+NF DAVDEIRGSLKQMRQ++        + SFRFQMSNVQTW
Sbjct: 98  ADYGADPRASSALHDCFSNFGDAVDEIRGSLKQMRQLSSTANSRGGSSSFRFQMSNVQTW 157

Query: 160 MSAALTDQETCTDGFDDVMDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRIP 209
           MSAALTD+ETCTDGFDDV DGP+K DV  + E VKK TSNALALVNS  EK  P
Sbjct: 158 MSAALTDEETCTDGFDDVEDGPLKTDVSNRVENVKKVTSNALALVNSVAEKGAP 211

The following BLAST results are available for this feature:
BLAST of CmaCh15G009690 vs. Swiss-Prot
Match NameE-valueIdentityDescription
21KD_DAUCA4.1e-3040.6121 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME26_ARATH1.1e-1428.14Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana GN=P... [more]
PME61_ARATH8.6e-1228.98Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=P... [more]
PME34_ARATH4.3e-1129.15Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=P... [more]
PMEU1_SOLLC2.8e-1026.19Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2... [more]
BLAST of CmaCh15G009690 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KPD9_CUCSA3.4e-9285.10Uncharacterized protein OS=Cucumis sativus GN=Csa_5G567800 PE=4 SV=1[more]
W9RGV6_9ROSA9.7e-6363.1121 kDa protein OS=Morus notabilis GN=L484_011998 PE=4 SV=1[more]
A0A061E7V9_THECC3.1e-6163.46Plant invertase/pectin methylesterase inhibitor superfamily protein (Fragment) O... [more]
A0A0D2NDN9_GOSRA2.6e-6071.69Uncharacterized protein OS=Gossypium raimondii GN=B456_005G160200 PE=4 SV=1[more]
A0A067FMZ7_CITSI1.3e-5960.00Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g028183mg PE=4 SV=1[more]
BLAST of CmaCh15G009690 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G23205.15.1e-4752.22 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G14890.11.6e-4553.40 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT2G01610.13.7e-4550.24 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G70720.17.2e-4150.56 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.13.3e-3040.74 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of CmaCh15G009690 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449464804|ref|XP_004150119.1|4.9e-9285.10PREDICTED: 21 kDa protein-like [Cucumis sativus][more]
gi|659072829|ref|XP_008467116.1|2.4e-9185.10PREDICTED: 21 kDa protein [Cucumis melo][more]
gi|703119893|ref|XP_010101980.1|1.4e-6263.1121 kDa protein [Morus notabilis][more]
gi|590696637|ref|XP_007045219.1|4.5e-6163.46Plant invertase/pectin methylesterase inhibitor superfamily protein, partial [Th... [more]
gi|694320679|ref|XP_009351520.1|7.7e-6171.84PREDICTED: 21 kDa protein-like [Pyrus x bretschneideri][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh15G009690.1CmaCh15G009690.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 40..202
score: 2.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 42..198
score: 1.6
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 38..198
score: 3.5
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 15..200
score: 6.5
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 40..202
score: 1.44
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 2..208
score: 3.7E
NoneNo IPR availablePANTHERPTHR31080:SF10PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR DOMAIN-CONTAINING PROTEINcoord: 2..208
score: 3.7E

Synteny