Carg23720 (gene) Silver-seed gourd

Overview
NameCarg23720
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description21 kDa protein
LocationCucurbita_argyrosperma_scaffold_213 : 62392 .. 63018 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACCCCAACCGTCTCCTATCGTCTCTCTCCTGTTCGCAGCCGGTCTCTTCGTCCTCCGCCTTGTTCCATCCGCTGCCGATGCCGGAACCTTTCCCAATGCCACCGCCGATTGTTCTGAGTTCATCCGCACTAGCTGCTTCATTACTCTCTATCCCGACGTCTGCTACACTTCGCTCTCTCGCTACGCCAATGCCATTCAGCAGGACCCGGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACGCCCGTAGAATGGCTGCTTATGTCTCCAACCTGTCTCACGTCGGTGACTACGGCGCGGACCACAGAGTCTCCTCCGCGCTTCACGACTGCTTCACGAACTTCGACGATGCCGTGGACGAGATTCGCGGCTCACTCAAGCAGATGCGTCAGATCAACGATGTCGACGCGCCGTCGTTTAGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCGGCGTTAACGGATCAAGAAACGTGTACGGACGGATTCGATGATGTGGTGGACGGACCGATTAAGAAGGATGTGTGCGCAAAGGCGGAGAAGGTGAAGAAACACACCAGTAATGCCTTAGCGTTGGTGAACAGCTTCGTGGAGAAAAGGATTCCGTGA

mRNA sequence

ATGAAACCCCAACCGTCTCCTATCGTCTCTCTCCTGTTCGCAGCCGGTCTCTTCGTCCTCCGCCTTGTTCCATCCGCTGCCGATGCCGGAACCTTTCCCAATGCCACCGCCGATTGTTCTGAGTTCATCCGCACTAGCTGCTTCATTACTCTCTATCCCGACGTCTGCTACACTTCGCTCTCTCGCTACGCCAATGCCATTCAGCAGGACCCGGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACGCCCGTAGAATGGCTGCTTATGTCTCCAACCTGTCTCACGTCGGTGACTACGGCGCGGACCACAGAGTCTCCTCCGCGCTTCACGACTGCTTCACGAACTTCGACGATGCCGTGGACGAGATTCGCGGCTCACTCAAGCAGATGCGTCAGATCAACGATGTCGACGCGCCGTCGTTTAGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCGGCGTTAACGGATCAAGAAACGTGTACGGACGGATTCGATGATGTGGTGGACGGACCGATTAAGAAGGATGTGTGCGCAAAGGCGGAGAAGGTGAAGAAACACACCAGTAATGCCTTAGCGTTGGTGAACAGCTTCGTGGAGAAAAGGATTCCGTGA

Coding sequence (CDS)

ATGAAACCCCAACCGTCTCCTATCGTCTCTCTCCTGTTCGCAGCCGGTCTCTTCGTCCTCCGCCTTGTTCCATCCGCTGCCGATGCCGGAACCTTTCCCAATGCCACCGCCGATTGTTCTGAGTTCATCCGCACTAGCTGCTTCATTACTCTCTATCCCGACGTCTGCTACACTTCGCTCTCTCGCTACGCCAATGCCATTCAGCAGGACCCGGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACGCCCGTAGAATGGCTGCTTATGTCTCCAACCTGTCTCACGTCGGTGACTACGGCGCGGACCACAGAGTCTCCTCCGCGCTTCACGACTGCTTCACGAACTTCGACGATGCCGTGGACGAGATTCGCGGCTCACTCAAGCAGATGCGTCAGATCAACGATGTCGACGCGCCGTCGTTTAGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCGGCGTTAACGGATCAAGAAACGTGTACGGACGGATTCGATGATGTGGTGGACGGACCGATTAAGAAGGATGTGTGCGCAAAGGCGGAGAAGGTGAAGAAACACACCAGTAATGCCTTAGCGTTGGTGAACAGCTTCGTGGAGAAAAGGATTCCGTGA

Protein sequence

MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRIP
Homology
[Close]
BLAST of Carg23720 vs. NCBI nr
Match: XP_022938421.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 408.3 bits (1048), Expect = 1.6e-110
Identity = 206/208 (99.04%), Postives = 207/208 (99.52%), Query Frame = 0 

Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQPSPIVSLLFAAGLF+LRLVPSAADAGTFPNATADCSEFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]
BLAST of Carg23720 vs. NCBI nr
Match: XP_023549992.1 (21 kDa protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 405.2 bits (1040), Expect = 1.3e-109
Identity = 205/208 (98.56%), Postives = 206/208 (99.04%), Query Frame = 0 

Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQPS IVSLLFAAGLF+LRLVPSAADAGTFPNATADCSEFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]
BLAST of Carg23720 vs. NCBI nr
Match: XP_022973542.1 (21 kDa protein-like [Cucurbita maxima])

HSP 1 Score: 401.7 bits (1031), Expect = 1.5e-108
Identity = 203/208 (97.60%), Postives = 205/208 (98.56%), Query Frame = 0 

Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQPS IVSLLFAAGLF+LRLVPSAADAGTFPNATADCSEFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHV DYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDV+DGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]
BLAST of Carg23720 vs. NCBI nr
Match: XP_023532575.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 349.7 bits (896), Expect = 6.6e-93
Identity = 179/210 (85.24%), Postives = 192/210 (91.43%), Query Frame = 0 

Query: 1   MKPQPSPIVSLLFAAGLFVLRLVP--SAADAGTFPNATADCSEFIRTSCFITLYPDVCYT 60
           MKPQ S I+SLLFAA L +LR VP  +AADAGT  NATA+ +EFIR+SC ITLYPDVCY 
Sbjct: 1   MKPQSSLILSLLFAATLCLLRQVPAVAAADAGTSVNATANSTEFIRSSCVITLYPDVCYA 60

Query: 61  SLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTN 120
           SLSRYAN IQQDPASLTR+AITISLAN+RRMA+YVSNLSHVGDYGADHR +SALHDCFTN
Sbjct: 61  SLSRYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTN 120

Query: 121 FDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIK 180
           FDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP+K
Sbjct: 121 FDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMK 180

Query: 181 KDVCAKAEKVKKHTSNALALVNSFVEKRIP 209
            DVCAKAEKVKKHTSNALALVNSFV+KRIP
Sbjct: 181 ADVCAKAEKVKKHTSNALALVNSFVQKRIP 210

[Close]
BLAST of Carg23720 vs. NCBI nr
Match: XP_004150119.1 (PREDICTED: 21 kDa protein-like [Cucumis sativus] >KGN51480.1 hypothetical protein Csa_5G567800 [Cucumis sativus])

HSP 1 Score: 348.6 bits (893), Expect = 1.5e-92
Identity = 178/208 (85.58%), Postives = 188/208 (90.38%), Query Frame = 0 

Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQ   I+SLLFAA LF LR V +  D    PNATA+C EFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQRPLILSLLFAATLFYLRPVSADEDTPNSPNATANCMEFIRTSCGITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYAN IQQDPASLTR+AITISLAN+RRMAAYVSNLSHVGD GAD R +SALHDCFTNFD
Sbjct: 61  SRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHVGDNGADRRAASALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP+K+D
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKED 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEK+IP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKKIP 208

[Close]
BLAST of Carg23720 vs. TAIR10
Match: AT1G23205.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 185.7 bits (470), Expect = 3.0e-47
Identity = 94/180 (52.22%), Postives = 134/180 (74.44%), Query Frame = 0 

Query: 34  NATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYV 93
           ++ +D S+FIRTSC  TLYPD+C++SLS +++++  DPA L R AI+++L     +A+Y+
Sbjct: 25  HSQSDDSDFIRTSCNTTLYPDLCFSSLSSFSSSVHNDPALLARAAISVTLTKTLDLASYL 84

Query: 94  SNLSHV----GDYGADHRVSSAL-HDCFTNFDDAVDEIRGSLKQMRQ-INDVDAPSFRFQ 153
           +N++ +     + GA H  ++A+ HDCF N  DAV+E++GS+KQMR+ ++     SFRFQ
Sbjct: 85  ANITTLQPESNEDGAHHPTAAAVFHDCFDNLKDAVEEMKGSMKQMRELVSTGSLESFRFQ 144

Query: 154 MSNVQTWMSAALTDQETCTDGFDDVVDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRI 208
           MSNVQTW+SAALTD+ETCTDGF D+ D P K D+CA+ + VKK TSNALALVN  V+K I
Sbjct: 145 MSNVQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKMTSNALALVNRCVDKAI 204

[Close]
BLAST of Carg23720 vs. TAIR10
Match: AT1G14890.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 179.5 bits (454), Expect = 2.1e-45
Identity = 100/191 (52.36%), Postives = 132/191 (69.11%), Query Frame = 0 

Query: 17  LFVLRLVPSAADAGTFP--NATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQDPASL 76
           LF+ R +     A  FP    T D  +FIRTSC  TLYPDVCYTSL+ YA+A+Q +PA L
Sbjct: 35  LFICRTI----SAVRFPPEQPTTDDLDFIRTSCNTTLYPDVCYTSLAGYASAVQDNPARL 94

Query: 77  TRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMR 136
            ++AI +SL+ A+  AAY+S LS           S+A+HDC +N  DAVD++RGSL+Q+R
Sbjct: 95  AKLAIGVSLSRAKYTAAYLSKLSR-------RAASAAVHDCVSNVGDAVDQMRGSLRQLR 154

Query: 137 QIND--VDAPSFRFQMSNVQTWMSAALTDQETCTDGF-DDVVDGPIKKDVCAKAEKVKKH 196
           ++N      P+FRFQMSNVQTWMSAALTD+ETCTDG  +++ DG  K  +C +   VK+ 
Sbjct: 155 EMNHRRPGDPAFRFQMSNVQTWMSAALTDEETCTDGVTEEMEDGETKTAICDRVADVKRF 214

Query: 197 TSNALALVNSF 203
           TSNALALVN++
Sbjct: 215 TSNALALVNTY 214

[Close]
BLAST of Carg23720 vs. TAIR10
Match: AT2G01610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 178.3 bits (451), Expect = 4.8e-45
Identity = 105/211 (49.76%), Postives = 140/211 (66.35%), Query Frame = 0 

Query: 11  LLFAAGLFVLRLVPSAADAGTFPN-ATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQ 70
           LL    +  +RL+P        PN  T +  +FIRTSC  TLYPDVC+TSLS YA+A+Q 
Sbjct: 19  LLIFGSISAVRLLPR-------PNTTTTNDLDFIRTSCNATLYPDVCFTSLSGYASAVQD 78

Query: 71  DPASLTRVAITISLANARRMAAYVSNLSH-VGDYGAD-HRVSSA-LHDCFTNFDDAVDEI 130
            PA L ++AI +SL+ A+  AA++S LS     Y  D H+ +SA + DC +N +DAVDE+
Sbjct: 79  SPARLAKLAIGVSLSQAKSTAAFLSKLSRSAAKYSGDGHQTASAVIRDCVSNVEDAVDEM 138

Query: 131 RGSLKQMRQIND--------VDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGP-I 190
           RGSL+Q+R +N             +FRFQMSNVQTWMSAALTD++TCTDGF+D+ +G  I
Sbjct: 139 RGSLRQLRDMNGRGGGTAARRSVETFRFQMSNVQTWMSAALTDEDTCTDGFEDMDEGGLI 198

Query: 191 KKDVCAKAEKVKKHTSNALALVNSFVEKRIP 209
           K  VC + E+VK+ TSNALALVN++     P
Sbjct: 199 KTTVCDRLEEVKRLTSNALALVNTYANNGAP 222

[Close]
BLAST of Carg23720 vs. TAIR10
Match: AT1G70720.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 164.9 bits (416), Expect = 5.5e-41
Identity = 90/178 (50.56%), Postives = 121/178 (67.98%), Query Frame = 0 

Query: 33  PNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAY 92
           P   ++ S+FIR +C  TLYPD+C+++LS +AN+IQ D   L RVAI+++L N   + +Y
Sbjct: 27  PLLPSNGSDFIRLACNTTLYPDLCFSTLSSFANSIQNDSNRLARVAISLTLHNTLHLLSY 86

Query: 93  VSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDA--PSFRFQMSN 152
           + N      Y  DH  +  L DCF N  DAVD +RGS+KQM+++        SFRFQMSN
Sbjct: 87  LQNA-----YNRDH-PTPVLRDCFENLKDAVDGMRGSMKQMKELVSASGSIESFRFQMSN 146

Query: 153 VQTWMSAALTDQETCTDGFDDV-VDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRI 208
           V+TW+SAALTD+ TCTDGF DV  D  IK DVC++ + VKK TSNALALVN + ++ I
Sbjct: 147 VKTWLSAALTDEYTCTDGFKDVHEDDSIKDDVCSRVDDVKKLTSNALALVNRYADESI 198

[Close]
BLAST of Carg23720 vs. TAIR10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 126.3 bits (316), Expect = 2.2e-29
Identity = 75/189 (39.68%), Postives = 107/189 (56.61%), Query Frame = 0 

Query: 18  FVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQDPASLTRV 77
           ++L L  +AA A +   A+     FI++SC  T YP +C  SLS YAN IQ  P  L   
Sbjct: 14  YLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAET 73

Query: 78  AITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQIN 137
           AI ++L+ A+    +VS L+ +   G   R   A+ DC    +D VD +  S+++++   
Sbjct: 74  AIAVTLSRAQSTKLFVSRLTRM--KGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCG 133

Query: 138 DV-DAPSFRFQMSNVQTWMSAALTDQETCTDGFDD-VVDGPIKKDVCAKAEKVKKHTSNA 197
              D   F + MSN QTW SAALTD+ TC+DGF   V+DG IK  V A+   V   TSNA
Sbjct: 134 SAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNA 193

Query: 198 LALVNSFVE 205
           L+L+N+F +
Sbjct: 194 LSLINAFAK 200

[Close]
BLAST of Carg23720 vs. Swiss-Prot
Match: sp|P17407|21KD_DAUCA (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.2e-29
Identity = 79/197 (40.10%), Postives = 115/197 (58.38%), Query Frame = 0 

Query: 10  SLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQ 69
           +L+F+A L +L    +AA A       A  ++FI+TSC +T YP VC  SLS YA  IQ 
Sbjct: 7   TLVFSALLVIL----AAASA-------APANQFIKTSCTLTTYPAVCEQSLSAYAKTIQN 66

Query: 70  DPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGS 129
           +P  L   A+ +SL   ++   ++  L+     G   R  +A+HDC    +D++D +  S
Sbjct: 67  NPQELASTALQVSLTRTQQAQTFMKRLNKF--KGLKARQYAAIHDCLEEVEDSLDRVSRS 126

Query: 130 LKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF-DDVVDGPIKKDVCAKAEKV 189
             +M+ ++      F F+MSNV+TW+SAALTD+ TC DGF    +DG IK+ V A+   V
Sbjct: 127 CDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAV 186

Query: 190 KKHTSNALALVNSFVEK 206
            + TSNALALVN+F  K
Sbjct: 187 ARVTSNALALVNNFAAK 190

[Close]
BLAST of Carg23720 vs. Swiss-Prot
Match: sp|Q9SB37|PMEI7_ARATH (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 7.3e-27
Identity = 76/193 (39.38%), Postives = 107/193 (55.44%), Query Frame = 0 

Query: 17  LFVLRLVPSA-ADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQDPASLT 76
           LFVL L  +A   A      ++  +EFI+ SC  T YPD C+ SLS YA+ I++ P  L 
Sbjct: 11  LFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLA 70

Query: 77  RVAITISLANARRMAAYVSNLSHVGDY-GADHRVSSALHDCFTNFDDAVDEIRGSLKQMR 136
             A+ +S+A A+    YVS ++   DY G   R   A+ DC     D VD +  SLK+++
Sbjct: 71  ETALAVSIARAKSAKTYVSEMT---DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELK 130

Query: 137 QINDVDA-PSFRFQMSNVQTWMSAALTDQETCTDGF-DDVVDGPIKKDVCAKAEKVKKHT 196
            + + D+   F F +SNV+TW SAALTD+  C DGF    + G +K  +      V + T
Sbjct: 131 HLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEET 190

Query: 197 SNALALVNSFVEK 206
           SNALAL+N F  K
Sbjct: 191 SNALALINDFASK 200

[Close]
BLAST of Carg23720 vs. Swiss-Prot
Match: sp|Q9SI74|PMI10_ARATH (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.6e-26
Identity = 64/162 (39.51%), Postives = 101/162 (62.35%), Query Frame = 0 

Query: 41  EFIRTSCFITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVG 100
           ++I+TSC ITLY  +CY SLS YA+ I+ +P  L  +A+ ++L++A+  + +V N+SH G
Sbjct: 146 DYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGG 205

Query: 101 DYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALT 160
             G       A+ DC     D+V  ++ S++++  IN  D+  F   MS+V+TW+SAALT
Sbjct: 206 --GLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALT 265

Query: 161 DQETCTDGFDDVVDGPIKKDVCAKAEKVKKHTSNALALVNSF 203
           + +TC DGF  +V   +K  V     +V + TSNALAL+N +
Sbjct: 266 NDDTCMDGF-SLVKTAVKDLVRRHVVEVARLTSNALALINMY 304

[Close]
BLAST of Carg23720 vs. Swiss-Prot
Match: sp|Q9STY5|PMI11_ARATH (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.2e-23
Identity = 65/166 (39.16%), Postives = 90/166 (54.22%), Query Frame = 0 

Query: 42  FIRTSCFITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGD 101
           FI+ SC  T YP VC  SL+ YAN+IQ  P  L   A+ +++  A+    +V  L     
Sbjct: 38  FIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRF-- 97

Query: 102 YGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDA-PSFRFQMSNVQTWMSAALT 161
                R   A+ DC     DAVD +  S+ +++          F F MSN QTW SAALT
Sbjct: 98  TSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALT 157

Query: 162 DQETCTDGF-DDVVDGPIKKDVCAKAEKVKKHTSNALALVNSFVEK 206
           +  TC+DGF   V+DG +K  V A+   + + TSNALAL+N+F +K
Sbjct: 158 NANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFAKK 201

[Close]
BLAST of Carg23720 vs. Swiss-Prot
Match: sp|Q9SI72|PMEI9_ARATH (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.9e-22
Identity = 72/193 (37.31%), Postives = 109/193 (56.48%), Query Frame = 0 

Query: 17  LFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQ-QDPASLT 76
           L +L  +     +   PN  ++  +FI +SC  T YP +C  +LS YA  I+  +   L 
Sbjct: 9   LVILLSITILQSSSATPN-RSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLA 68

Query: 77  RVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQM-R 136
           + A+ ISLA A+ +  +V+ L+         R   A+ DC     ++VD +  S+K++ R
Sbjct: 69  QTALIISLARAKSVTIFVAKLTKETP-KFKRREYLAIKDCIEVLGNSVDRLAQSVKELAR 128

Query: 137 QINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDD-VVDGPIKKDVCAKAEKVKKHTS 196
             + V +  F ++MSNVQTW+SAALTD+ TC DGF +  + G +K+ +  K   V + TS
Sbjct: 129 AGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTS 188

Query: 197 NALALVNSFVEKR 207
           NALALVN F EKR
Sbjct: 189 NALALVNQFAEKR 199

[Close]
BLAST of Carg23720 vs. TrEMBL
Match: tr|A0A0A0KPD9|A0A0A0KPD9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G567800 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 9.8e-93
Identity = 178/208 (85.58%), Postives = 188/208 (90.38%), Query Frame = 0 

Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQ   I+SLLFAA LF LR V +  D    PNATA+C EFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQRPLILSLLFAATLFYLRPVSADEDTPNSPNATANCMEFIRTSCGITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYAN IQQDPASLTR+AITISLAN+RRMAAYVSNLSHVGD GAD R +SALHDCFTNFD
Sbjct: 61  SRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHVGDNGADRRAASALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP+K+D
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKED 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEK+IP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKKIP 208

[Close]
BLAST of Carg23720 vs. TrEMBL
Match: tr|A0A1S3CSS6|A0A1S3CSS6_CUCME (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103504548 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 4.8e-92
Identity = 178/208 (85.58%), Postives = 187/208 (89.90%), Query Frame = 0 

Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQ   I+SLLFAA LF LR V +  D     NATA+C EFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQRPLILSLLFAATLFHLRPVFANEDTRNSSNATANCIEFIRTSCGITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYAN IQQDPASLTR+AITISLAN+RRMAAYVSNLSH GDYGAD R +SALHDCFTNFD
Sbjct: 61  SRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHAGDYGADRRAASALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP+K+D
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKED 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]
BLAST of Carg23720 vs. TrEMBL
Match: tr|A0A2N9ETL4|A0A2N9ETL4_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6015 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.1e-67
Identity = 136/203 (67.00%), Postives = 163/203 (80.30%), Query Frame = 0 

Query: 8   IVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAI 67
           +VSL+ A  LF L+ +P+  ++   P  T + ++FIRTSC  TLYP++CY SLSRYANAI
Sbjct: 6   LVSLVLATLLFHLQPIPAVGNSS--PTTTTNGTDFIRTSCGTTLYPELCYNSLSRYANAI 65

Query: 68  QQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIR 127
           QQ+PA L +V+I++SLA ARRMAAYVSNL+   DYG+DHR +SALHDCFTNFDDAVDEIR
Sbjct: 66  QQNPAQLAKVSISVSLAKARRMAAYVSNLARQADYGSDHRAASALHDCFTNFDDAVDEIR 125

Query: 128 GSLKQMRQINDVDA--PSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKDVCAKA 187
           GSLKQMRQ+    A   SFRFQMSNVQTWMSAALTD++TCTDGF+DV DGP+K DVC + 
Sbjct: 126 GSLKQMRQLGAAGAGGVSFRFQMSNVQTWMSAALTDEDTCTDGFEDVADGPLKTDVCDRV 185

Query: 188 EKVKKHTSNALALVNSFVEKRIP 209
            KV K TSNALALVNS+VEK  P
Sbjct: 186 GKVHKFTSNALALVNSYVEKGTP 206

[Close]
BLAST of Carg23720 vs. TrEMBL
Match: tr|A0A2I4G1T4|A0A2I4G1T4_9ROSI (21 kDa protein-like OS=Juglans regia OX=51240 GN=LOC109003963 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 3.5e-66
Identity = 133/200 (66.50%), Postives = 155/200 (77.50%), Query Frame = 0 

Query: 9   VSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQ 68
           +SL  A  LF LR +P+  D G  P  TA+ ++FIR SC  TLYPD+CYTSLSRYANAIQ
Sbjct: 11  LSLCLATLLFGLRPIPAVGDTG--PVTTANATDFIRNSCGTTLYPDLCYTSLSRYANAIQ 70

Query: 69  QDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRG 128
           Q+PA L +VAI +SLA ARR+A+YVSN+S   DYG D    S LHDCFTNF DAVDE+RG
Sbjct: 71  QNPAQLAKVAIGVSLAKARRIASYVSNVSRHSDYGTDPLTVSVLHDCFTNFGDAVDEMRG 130

Query: 129 SLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKDVCAKAEKV 188
           SL+QMRQ+    A SFRFQM NVQTWMSAALTD+ETCTDGF+DV DGP+K D+C +  KV
Sbjct: 131 SLQQMRQLGAAGAESFRFQMGNVQTWMSAALTDEETCTDGFEDVKDGPVKTDMCGRVAKV 190

Query: 189 KKHTSNALALVNSFVEKRIP 209
           KK TSNALALVNS+V K IP
Sbjct: 191 KKFTSNALALVNSYVSKGIP 208

[Close]
BLAST of Carg23720 vs. TrEMBL
Match: tr|A0A2C9U641|A0A2C9U641_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_17G081300 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.9e-64
Identity = 129/203 (63.55%), Postives = 158/203 (77.83%), Query Frame = 0 

Query: 8   IVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAI 67
           I+SLLFA  L  L+ + + A A   P   ++ +++IRTSC  TLYP++C+TSLSRYA+A+
Sbjct: 10  ILSLLFATFLLHLQPIATVASAYPAPAPQSNSTDYIRTSCGATLYPEICFTSLSRYASAV 69

Query: 68  QQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIR 127
           QQDPA L RVAI +SL+ AR MA YVSN+S   DYG+DHR ++ALHDC++NF DAVDEI 
Sbjct: 70  QQDPARLARVAIGVSLSRARHMAVYVSNISREADYGSDHRAAAALHDCYSNFGDAVDEIH 129

Query: 128 GSLKQMRQINDV--DAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKDVCAKA 187
           GSLKQMRQI      A +FRFQMSNVQTWMSAALTD+ETCTDGF+DV DGP+K DVC +A
Sbjct: 130 GSLKQMRQIGAAGSSAEAFRFQMSNVQTWMSAALTDEETCTDGFEDVPDGPVKSDVCERA 189

Query: 188 EKVKKHTSNALALVNSFVEKRIP 209
             VKK TSNALALVNS+  K +P
Sbjct: 190 ADVKKFTSNALALVNSYAAKGMP 212

The following BLAST results are available for this feature:
BLAST of Carg23720 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022938421.11.6e-11099.0421 kDa protein-like [Cucurbita moschata][more]
XP_023549992.11.3e-10998.5621 kDa protein [Cucurbita pepo subsp. pepo][more]
XP_022973542.11.5e-10897.6021 kDa protein-like [Cucurbita maxima][more]
XP_023532575.16.6e-9385.2421 kDa protein-like [Cucurbita pepo subsp. pepo][more]
XP_004150119.11.5e-9285.58PREDICTED: 21 kDa protein-like [Cucumis sativus] >KGN51480.1 hypothetical protei... [more]
BLAST of Carg23720 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G23205.13.0e-4752.22Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G14890.12.1e-4552.36Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT2G01610.14.8e-4549.76Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G70720.15.5e-4150.56Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.12.2e-2939.68Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of Carg23720 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|P17407|21KD_DAUCA1.2e-2940.1021 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1[more]
sp|Q9SB37|PMEI7_ARATH7.3e-2739.38Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1[more]
sp|Q9SI74|PMI10_ARATH3.6e-2639.51Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1[more]
sp|Q9STY5|PMI11_ARATH2.2e-2339.16Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1[more]
sp|Q9SI72|PMEI9_ARATH1.9e-2237.31Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1[more]
BLAST of Carg23720 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0KPD9|A0A0A0KPD9_CUCSA9.8e-9385.58Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G567800 PE=4 SV=1[more]
tr|A0A1S3CSS6|A0A1S3CSS6_CUCME4.8e-9285.5821 kDa protein OS=Cucumis melo OX=3656 GN=LOC103504548 PE=4 SV=1[more]
tr|A0A2N9ETL4|A0A2N9ETL4_FAGSY1.1e-6767.00Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6015 PE=4 SV=1[more]
tr|A0A2I4G1T4|A0A2I4G1T4_9ROSI3.5e-6666.5021 kDa protein-like OS=Juglans regia OX=51240 GN=LOC109003963 PE=4 SV=1[more]
tr|A0A2C9U641|A0A2C9U641_MANES1.9e-6463.55Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_17G081300 PE=4 SV=... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR035513Invertase/methylesterase_inhib
IPR006501Pectinesterase_inhib_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23720-RACarg23720-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 38..198
e-value: 9.3E-44
score: 161.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 42..198
e-value: 3.0E-39
score: 134.7
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 16..200
e-value: 1.5E-30
score: 103.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3DG3DSA:1.20.140.40coord: 39..203
e-value: 4.9E-26
score: 93.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILYSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 40..202
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 6..203
NoneNo IPR availablePANTHERPTHR31080:SF10PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR DOMAIN-CONTAINING PROTEINcoord: 6..203
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 45..202
e-value: 1.09541E-46
score: 152.207