Carg26064 (gene) Silver-seed gourd

NameCarg26064
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description21 kDa protein
LocationCucurbita_argyrosperma_scaffold_258 : 213402 .. 214037 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGACCTCAATCGCCTCTTATCCTCTCTCTCCTATTCGTAGCCACTCTCTGTCTCCTACGCCAGGTTCCGGCAGCCGCCGATGCTGCTGATGCTGGGACCTCCGTCAACGCCACTGCCAATAGTACCGAGTTTATCCGGAGTAGCTGTGTCATTACTCTCTATCCCGACGTCTGCTACGCTTCGCTCTCTCGCTATGCTAACGACATTCAGCAGGATCCAGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACTCCCGTAGAATGGCTTCTTATGTATCCAACCTGTCTCACGTCGGAGACTACGGCGCCGACCACAGAGCCGCCTCCGCGCTTCACGATTGCTTCACGAACTTCGACGACGCCGTCGACGAGATTCGCGGCTCGCTCAACCAGATGCGCCAGATCAACGACGTCGATGTGCCGTCGTTCAGGTTTCAAATGAGCAACGTGCAGACGTGGATGAGCGCCGCGCTTACGGATCAGGAAACATGTACGGACGGATTCGAAGATGTGGCCGATGGACCGATGAAAGCGGACGTATGCGCAAAGGCGGAGAAGGTGAAGAAGCACACTAGTAATGCCCTAGCGCTGGTGAATAGCTTCGTCCAGAAAAGGATTCCGTGA

mRNA sequence

ATGAGACCTCAATCGCCTCTTATCCTCTCTCTCCTATTCGTAGCCACTCTCTGTCTCCTACGCCAGGTTCCGGCAGCCGCCGATGCTGCTGATGCTGGGACCTCCGTCAACGCCACTGCCAATAGTACCGAGTTTATCCGGAGTAGCTGTGTCATTACTCTCTATCCCGACGTCTGCTACGCTTCGCTCTCTCGCTATGCTAACGACATTCAGCAGGATCCAGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACTCCCGTAGAATGGCTTCTTATGTATCCAACCTGTCTCACGTCGGAGACTACGGCGCCGACCACAGAGCCGCCTCCGCGCTTCACGATTGCTTCACGAACTTCGACGACGCCGTCGACGAGATTCGCGGCTCGCTCAACCAGATGCGCCAGATCAACGACGTCGATGTGCCGTCGTTCAGGTTTCAAATGAGCAACGTGCAGACGTGGATGAGCGCCGCGCTTACGGATCAGGAAACATGTACGGACGGATTCGAAGATGTGGCCGATGGACCGATGAAAGCGGACGTATGCGCAAAGGCGGAGAAGGTGAAGAAGCACACTAGTAATGCCCTAGCGCTGGTGAATAGCTTCGTCCAGAAAAGGATTCCGTGA

Coding sequence (CDS)

ATGAGACCTCAATCGCCTCTTATCCTCTCTCTCCTATTCGTAGCCACTCTCTGTCTCCTACGCCAGGTTCCGGCAGCCGCCGATGCTGCTGATGCTGGGACCTCCGTCAACGCCACTGCCAATAGTACCGAGTTTATCCGGAGTAGCTGTGTCATTACTCTCTATCCCGACGTCTGCTACGCTTCGCTCTCTCGCTATGCTAACGACATTCAGCAGGATCCAGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACTCCCGTAGAATGGCTTCTTATGTATCCAACCTGTCTCACGTCGGAGACTACGGCGCCGACCACAGAGCCGCCTCCGCGCTTCACGATTGCTTCACGAACTTCGACGACGCCGTCGACGAGATTCGCGGCTCGCTCAACCAGATGCGCCAGATCAACGACGTCGATGTGCCGTCGTTCAGGTTTCAAATGAGCAACGTGCAGACGTGGATGAGCGCCGCGCTTACGGATCAGGAAACATGTACGGACGGATTCGAAGATGTGGCCGATGGACCGATGAAAGCGGACGTATGCGCAAAGGCGGAGAAGGTGAAGAAGCACACTAGTAATGCCCTAGCGCTGGTGAATAGCTTCGTCCAGAAAAGGATTCCGTGA

Protein sequence

MRPQSPLILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKADVCAKAEKVKKHTSNALALVNSFVQKRIP
BLAST of Carg26064 vs. NCBI nr
Match: XP_022962658.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 407.1 bits (1045), Expect = 3.5e-110
Identity = 209/211 (99.05%), Postives = 211/211 (100.00%), Query Frame = 0

Query: 1   MRPQSPLILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCY 60
           M+PQSPLILSLLFVATLCLLRQVPAAADAAD+GTSVNATANSTEFIRSSCVITLYPDVCY
Sbjct: 1   MKPQSPLILSLLFVATLCLLRQVPAAADAADSGTSVNATANSTEFIRSSCVITLYPDVCY 60

Query: 61  ASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT 120
           ASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT
Sbjct: 61  ASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT 120

Query: 121 NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180
           NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM
Sbjct: 121 NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180

Query: 181 KADVCAKAEKVKKHTSNALALVNSFVQKRIP 212
           KADVCAKAEKVKKHTSNALALVNSFVQKRIP
Sbjct: 181 KADVCAKAEKVKKHTSNALALVNSFVQKRIP 211

BLAST of Carg26064 vs. NCBI nr
Match: XP_022990704.1 (21 kDa protein-like [Cucurbita maxima])

HSP 1 Score: 397.5 bits (1020), Expect = 2.8e-107
Identity = 207/212 (97.64%), Postives = 208/212 (98.11%), Query Frame = 0

Query: 1   MRPQSPLILSLLFVATLCLLRQVPAAADA-ADAGTSVNATANSTEFIRSSCVITLYPDVC 60
           M+PQSPLILSLLF ATLCLLRQVPAAA A ADAGTSVNATAN TEFIRSSCVITLYPDVC
Sbjct: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAXADAGTSVNATANGTEFIRSSCVITLYPDVC 60

Query: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120
           YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF
Sbjct: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120

Query: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180
           TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP
Sbjct: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180

Query: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 212
           MKADVCAKAEKVKKHTSNALALVNSFVQKRIP
Sbjct: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 212

BLAST of Carg26064 vs. NCBI nr
Match: XP_023532575.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394.8 bits (1013), Expect = 1.8e-106
Identity = 205/211 (97.16%), Postives = 207/211 (98.10%), Query Frame = 0

Query: 1   MRPQSPLILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCY 60
           M+PQS LILSLLF ATLCLLRQVPA A AADAGTSVNATANSTEFIRSSCVITLYPDVCY
Sbjct: 1   MKPQSSLILSLLFAATLCLLRQVPAVA-AADAGTSVNATANSTEFIRSSCVITLYPDVCY 60

Query: 61  ASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT 120
           ASLSRYANDIQQDPASLTR+AITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT
Sbjct: 61  ASLSRYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT 120

Query: 121 NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180
           NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM
Sbjct: 121 NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180

Query: 181 KADVCAKAEKVKKHTSNALALVNSFVQKRIP 212
           KADVCAKAEKVKKHTSNALALVNSFVQKRIP
Sbjct: 181 KADVCAKAEKVKKHTSNALALVNSFVQKRIP 210

BLAST of Carg26064 vs. NCBI nr
Match: XP_008467116.1 (PREDICTED: 21 kDa protein [Cucumis melo])

HSP 1 Score: 345.9 bits (886), Expect = 9.7e-92
Identity = 182/211 (86.26%), Postives = 188/211 (89.10%), Query Frame = 0

Query: 1   MRPQSPLILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCY 60
           M+PQ PLILSLLF ATL  LR V A  D  +   S NATAN  EFIR+SC ITLYPDVCY
Sbjct: 1   MKPQRPLILSLLFAATLFHLRPVFANEDTRN---SSNATANCIEFIRTSCGITLYPDVCY 60

Query: 61  ASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT 120
            SLSRYANDIQQDPASLTR+AITISLANSRRMA+YVSNLSH GDYGAD RAASALHDCFT
Sbjct: 61  TSLSRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHAGDYGADRRAASALHDCFT 120

Query: 121 NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180
           NFDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM
Sbjct: 121 NFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180

Query: 181 KADVCAKAEKVKKHTSNALALVNSFVQKRIP 212
           K DVCAKAEKVKKHTSNALALVNSFV+KRIP
Sbjct: 181 KEDVCAKAEKVKKHTSNALALVNSFVEKRIP 208

BLAST of Carg26064 vs. NCBI nr
Match: XP_004150119.1 (PREDICTED: 21 kDa protein-like [Cucumis sativus] >KGN51480.1 hypothetical protein Csa_5G567800 [Cucumis sativus])

HSP 1 Score: 345.1 bits (884), Expect = 1.7e-91
Identity = 182/211 (86.26%), Postives = 189/211 (89.57%), Query Frame = 0

Query: 1   MRPQSPLILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCY 60
           M+PQ PLILSLLF ATL  LR  P +AD  D   S NATAN  EFIR+SC ITLYPDVCY
Sbjct: 1   MKPQRPLILSLLFAATLFYLR--PVSAD-EDTPNSPNATANCMEFIRTSCGITLYPDVCY 60

Query: 61  ASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT 120
            SLSRYANDIQQDPASLTR+AITISLANSRRMA+YVSNLSHVGD GAD RAASALHDCFT
Sbjct: 61  TSLSRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHVGDNGADRRAASALHDCFT 120

Query: 121 NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180
           NFDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM
Sbjct: 121 NFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180

Query: 181 KADVCAKAEKVKKHTSNALALVNSFVQKRIP 212
           K DVCAKAEKVKKHTSNALALVNSFV+K+IP
Sbjct: 181 KEDVCAKAEKVKKHTSNALALVNSFVEKKIP 208

BLAST of Carg26064 vs. TAIR10
Match: AT1G23205.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 184.5 bits (467), Expect = 6.8e-47
Identity = 93/182 (51.10%), Postives = 135/182 (74.18%), Query Frame = 0

Query: 35  SVNATANSTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITISLANSRRMAS 94
           +V++ ++ ++FIR+SC  TLYPD+C++SLS +++ +  DPA L R AI+++L  +  +AS
Sbjct: 23  TVHSQSDDSDFIRTSCNTTLYPDLCFSSLSSFSSSVHNDPALLARAAISVTLTKTLDLAS 82

Query: 95  YVSNLSHV----GDYGADH-RAASALHDCFTNFDDAVDEIRGSLNQMRQ-INDVDVPSFR 154
           Y++N++ +     + GA H  AA+  HDCF N  DAV+E++GS+ QMR+ ++   + SFR
Sbjct: 83  YLANITTLQPESNEDGAHHPTAAAVFHDCFDNLKDAVEEMKGSMKQMRELVSTGSLESFR 142

Query: 155 FQMSNVQTWMSAALTDQETCTDGFEDVADGPMKADVCAKAEKVKKHTSNALALVNSFVQK 211
           FQMSNVQTW+SAALTD+ETCTDGF+D+ D P K D+CA+ + VKK TSNALALVN  V K
Sbjct: 143 FQMSNVQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKMTSNALALVNRCVDK 202

BLAST of Carg26064 vs. TAIR10
Match: AT2G01610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 171.0 bits (432), Expect = 7.8e-43
Identity = 103/220 (46.82%), Postives = 138/220 (62.73%), Query Frame = 0

Query: 4   QSPLILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCYASL 63
           QS     L F   L +   + A        T+   T N  +FIR+SC  TLYPDVC+ SL
Sbjct: 6   QSHTTTFLFFTTFLLIFGSISAVRLLPRPNTT---TTNDLDFIRTSCNATLYPDVCFTSL 65

Query: 64  SRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSH-VGDYGAD-HRAASA-LHDCFT 123
           S YA+ +Q  PA L ++AI +SL+ ++  A+++S LS     Y  D H+ ASA + DC +
Sbjct: 66  SGYASAVQDSPARLAKLAIGVSLSQAKSTAAFLSKLSRSAAKYSGDGHQTASAVIRDCVS 125

Query: 124 NFDDAVDEIRGSLNQMRQIND--------VDVPSFRFQMSNVQTWMSAALTDQETCTDGF 183
           N +DAVDE+RGSL Q+R +N           V +FRFQMSNVQTWMSAALTD++TCTDGF
Sbjct: 126 NVEDAVDEMRGSLRQLRDMNGRGGGTAARRSVETFRFQMSNVQTWMSAALTDEDTCTDGF 185

Query: 184 EDVADGPM-KADVCAKAEKVKKHTSNALALVNSFVQKRIP 212
           ED+ +G + K  VC + E+VK+ TSNALALVN++     P
Sbjct: 186 EDMDEGGLIKTTVCDRLEEVKRLTSNALALVNTYANNGAP 222

BLAST of Carg26064 vs. TAIR10
Match: AT1G14890.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 170.2 bits (430), Expect = 1.3e-42
Identity = 89/170 (52.35%), Postives = 122/170 (71.76%), Query Frame = 0

Query: 39  TANSTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSN 98
           T +  +FIR+SC  TLYPDVCY SL+ YA+ +Q +PA L ++AI +SL+ ++  A+Y+S 
Sbjct: 52  TTDDLDFIRTSCNTTLYPDVCYTSLAGYASAVQDNPARLAKLAIGVSLSRAKYTAAYLSK 111

Query: 99  LSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQIND--VDVPSFRFQMSNVQT 158
           LS          A++A+HDC +N  DAVD++RGSL Q+R++N      P+FRFQMSNVQT
Sbjct: 112 LSR-------RAASAAVHDCVSNVGDAVDQMRGSLRQLREMNHRRPGDPAFRFQMSNVQT 171

Query: 159 WMSAALTDQETCTDGF-EDVADGPMKADVCAKAEKVKKHTSNALALVNSF 206
           WMSAALTD+ETCTDG  E++ DG  K  +C +   VK+ TSNALALVN++
Sbjct: 172 WMSAALTDEETCTDGVTEEMEDGETKTAICDRVADVKRFTSNALALVNTY 214

BLAST of Carg26064 vs. TAIR10
Match: AT1G70720.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 164.1 bits (414), Expect = 9.5e-41
Identity = 88/174 (50.57%), Postives = 119/174 (68.39%), Query Frame = 0

Query: 40  ANSTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNL 99
           +N ++FIR +C  TLYPD+C+++LS +AN IQ D   L RVAI+++L N+  + SY+ N 
Sbjct: 31  SNGSDFIRLACNTTLYPDLCFSTLSSFANSIQNDSNRLARVAISLTLHNTLHLLSYLQNA 90

Query: 100 SHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDV--DVPSFRFQMSNVQTW 159
                Y  DH     L DCF N  DAVD +RGS+ QM+++      + SFRFQMSNV+TW
Sbjct: 91  -----YNRDH-PTPVLRDCFENLKDAVDGMRGSMKQMKELVSASGSIESFRFQMSNVKTW 150

Query: 160 MSAALTDQETCTDGFEDV-ADGPMKADVCAKAEKVKKHTSNALALVNSFVQKRI 211
           +SAALTD+ TCTDGF+DV  D  +K DVC++ + VKK TSNALALVN +  + I
Sbjct: 151 LSAALTDEYTCTDGFKDVHEDDSIKDDVCSRVDDVKKLTSNALALVNRYADESI 198

BLAST of Carg26064 vs. TAIR10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 125.2 bits (313), Expect = 4.9e-29
Identity = 73/181 (40.33%), Postives = 102/181 (56.35%), Query Frame = 0

Query: 29  AADAGTSVNATANSTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITISLAN 88
           AA A +   A+  +  FI+SSC  T YP +C  SLS YANDIQ  P  L   AI ++L+ 
Sbjct: 22  AATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSR 81

Query: 89  SRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDV-DVPSF 148
           ++    +VS L+ +   G   R   A+ DC    +D VD +  S+ +++      D   F
Sbjct: 82  AQSTKLFVSRLTRM--KGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQF 141

Query: 149 RFQMSNVQTWMSAALTDQETCTDGFED-VADGPMKADVCAKAEKVKKHTSNALALVNSFV 208
            + MSN QTW SAALTD+ TC+DGF   V DG +K  V A+   V   TSNAL+L+N+F 
Sbjct: 142 AYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLINAFA 200

BLAST of Carg26064 vs. Swiss-Prot
Match: sp|P17407|21KD_DAUCA (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.8e-28
Identity = 79/200 (39.50%), Postives = 115/200 (57.50%), Query Frame = 0

Query: 10  SLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCYASLSRYAND 69
           +L+F A L +L    AAA AA A           +FI++SC +T YP VC  SLS YA  
Sbjct: 7   TLVFSALLVIL----AAASAAPA----------NQFIKTSCTLTTYPAVCEQSLSAYAKT 66

Query: 70  IQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEI 129
           IQ +P  L   A+ +SL  +++  +++  L+     G   R  +A+HDC    +D++D +
Sbjct: 67  IQNNPQELASTALQVSLTRTQQAQTFMKRLNKF--KGLKARQYAAIHDCLEEVEDSLDRV 126

Query: 130 RGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVA-DGPMKADVCAKA 189
             S ++M+ ++      F F+MSNV+TW+SAALTD+ TC DGF     DG +K  V A+ 
Sbjct: 127 SRSCDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQV 186

Query: 190 EKVKKHTSNALALVNSFVQK 209
             V + TSNALALVN+F  K
Sbjct: 187 VAVARVTSNALALVNNFAAK 190

BLAST of Carg26064 vs. Swiss-Prot
Match: sp|Q9SB37|PMEI7_ARATH (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.4e-25
Identity = 75/205 (36.59%), Postives = 113/205 (55.12%), Query Frame = 0

Query: 7   LILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCYASLSRY 66
           LIL +L+++T  ++           A      ++  TEFI++SC  T YPD C+ SLS Y
Sbjct: 9   LILFVLYLSTAAIVM----------ARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSY 68

Query: 67  ANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDY-GADHRAASALHDCFTNFDDA 126
           A++I++ P  L   A+ +S+A ++   +YVS ++   DY G   R   A+ DC     D 
Sbjct: 69  ASEIKKQPRKLAETALAVSIARAKSAKTYVSEMT---DYKGITKRQHEAVADCLEEMGDT 128

Query: 127 VDEIRGSLNQMRQINDVDV-PSFRFQMSNVQTWMSAALTDQETCTDGFEDVA-DGPMKAD 186
           VD +  SL +++ + + D    F F +SNV+TW SAALTD+  C DGF   A  G +K+ 
Sbjct: 129 VDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSL 188

Query: 187 VCAKAEKVKKHTSNALALVNSFVQK 209
           +      V + TSNALAL+N F  K
Sbjct: 189 IRTHIVSVAEETSNALALINDFASK 200

BLAST of Carg26064 vs. Swiss-Prot
Match: sp|Q9SI74|PMI10_ARATH (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 5.9e-24
Identity = 62/162 (38.27%), Postives = 98/162 (60.49%), Query Frame = 0

Query: 44  EFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVG 103
           ++I++SC ITLY  +CY SLS YA+ I+ +P  L  +A+ ++L++++  + +V N+SH G
Sbjct: 146 DYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGG 205

Query: 104 DYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALT 163
             G       A+ DC     D+V  ++ S+ ++  IN  D   F   MS+V+TW+SAALT
Sbjct: 206 --GLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALT 265

Query: 164 DQETCTDGFEDVADGPMKADVCAKAEKVKKHTSNALALVNSF 206
           + +TC DGF  V    +K  V     +V + TSNALAL+N +
Sbjct: 266 NDDTCMDGFSLVKTA-VKDLVRRHVVEVARLTSNALALINMY 304

BLAST of Carg26064 vs. Swiss-Prot
Match: sp|Q9STY5|PMI11_ARATH (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.6e-21
Identity = 73/204 (35.78%), Postives = 101/204 (49.51%), Query Frame = 0

Query: 7   LILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCYASLSRY 66
           L L LL  A L      P AA           +  +  FI++SC  T YP VC  SL+ Y
Sbjct: 9   LFLFLLSTAILTASSSAPRAA---------ITSKRAINFIQASCKATTYPTVCVNSLTGY 68

Query: 67  ANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAV 126
           AN IQ  P  L   A+ +++  ++    +V  L          R   A+ DC     DAV
Sbjct: 69  ANSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRF--TSLKKREIQAVKDCIEEIHDAV 128

Query: 127 DEIRGSLNQMRQINDV-DVPSFRFQMSNVQTWMSAALTDQETCTDGFED-VADGPMKADV 186
           D +  S+++++          F F MSN QTW SAALT+  TC+DGF   V DG +K  V
Sbjct: 129 DRLTMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSV 188

Query: 187 CAKAEKVKKHTSNALALVNSFVQK 209
            A+   + + TSNALAL+N+F +K
Sbjct: 189 RARILNLGRGTSNALALINAFAKK 201

BLAST of Carg26064 vs. Swiss-Prot
Match: sp|Q9SI72|PMEI9_ARATH (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.8e-20
Identity = 72/181 (39.78%), Postives = 103/181 (56.91%), Query Frame = 0

Query: 35  SVNATAN---STEFIRSSCVITLYPDVCYASLSRYANDIQ-QDPASLTRVAITISLANSR 94
           S +AT N   S +FI SSC  T YP +C  +LS YA  I+  +   L + A+ ISLA ++
Sbjct: 20  SSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTALIISLARAK 79

Query: 95  RMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQM-RQINDVDVPSFRF 154
            +  +V+ L+         R   A+ DC     ++VD +  S+ ++ R  + V    F +
Sbjct: 80  SVTIFVAKLTKETP-KFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHAVASEDFMW 139

Query: 155 QMSNVQTWMSAALTDQETCTDGFEDVA-DGPMKADVCAKAEKVKKHTSNALALVNSFVQK 210
           +MSNVQTW+SAALTD+ TC DGF + A  G +K  +  K   V + TSNALALVN F +K
Sbjct: 140 KMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNALALVNQFAEK 199

BLAST of Carg26064 vs. TrEMBL
Match: tr|A0A1S3CSS6|A0A1S3CSS6_CUCME (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103504548 PE=4 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 6.4e-92
Identity = 182/211 (86.26%), Postives = 188/211 (89.10%), Query Frame = 0

Query: 1   MRPQSPLILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCY 60
           M+PQ PLILSLLF ATL  LR V A  D  +   S NATAN  EFIR+SC ITLYPDVCY
Sbjct: 1   MKPQRPLILSLLFAATLFHLRPVFANEDTRN---SSNATANCIEFIRTSCGITLYPDVCY 60

Query: 61  ASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT 120
            SLSRYANDIQQDPASLTR+AITISLANSRRMA+YVSNLSH GDYGAD RAASALHDCFT
Sbjct: 61  TSLSRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHAGDYGADRRAASALHDCFT 120

Query: 121 NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180
           NFDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM
Sbjct: 121 NFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180

Query: 181 KADVCAKAEKVKKHTSNALALVNSFVQKRIP 212
           K DVCAKAEKVKKHTSNALALVNSFV+KRIP
Sbjct: 181 KEDVCAKAEKVKKHTSNALALVNSFVEKRIP 208

BLAST of Carg26064 vs. TrEMBL
Match: tr|A0A0A0KPD9|A0A0A0KPD9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G567800 PE=4 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.1e-91
Identity = 182/211 (86.26%), Postives = 189/211 (89.57%), Query Frame = 0

Query: 1   MRPQSPLILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCY 60
           M+PQ PLILSLLF ATL  LR  P +AD  D   S NATAN  EFIR+SC ITLYPDVCY
Sbjct: 1   MKPQRPLILSLLFAATLFYLR--PVSAD-EDTPNSPNATANCMEFIRTSCGITLYPDVCY 60

Query: 61  ASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT 120
            SLSRYANDIQQDPASLTR+AITISLANSRRMA+YVSNLSHVGD GAD RAASALHDCFT
Sbjct: 61  TSLSRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHVGDNGADRRAASALHDCFT 120

Query: 121 NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180
           NFDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM
Sbjct: 121 NFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180

Query: 181 KADVCAKAEKVKKHTSNALALVNSFVQKRIP 212
           K DVCAKAEKVKKHTSNALALVNSFV+K+IP
Sbjct: 181 KEDVCAKAEKVKKHTSNALALVNSFVEKKIP 208

BLAST of Carg26064 vs. TrEMBL
Match: tr|A0A2N9ETL4|A0A2N9ETL4_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6015 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.1e-64
Identity = 133/206 (64.56%), Postives = 160/206 (77.67%), Query Frame = 0

Query: 8   ILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCYASLSRYA 67
           ++SL+    L  L+ +PA  +     +S   T N T+FIR+SC  TLYP++CY SLSRYA
Sbjct: 6   LVSLVLATLLFHLQPIPAVGN-----SSPTTTTNGTDFIRTSCGTTLYPELCYNSLSRYA 65

Query: 68  NDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVD 127
           N IQQ+PA L +V+I++SLA +RRMA+YVSNL+   DYG+DHRAASALHDCFTNFDDAVD
Sbjct: 66  NAIQQNPAQLAKVSISVSLAKARRMAAYVSNLARQADYGSDHRAASALHDCFTNFDDAVD 125

Query: 128 EIRGSLNQMRQINDVDV--PSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKADVC 187
           EIRGSL QMRQ+        SFRFQMSNVQTWMSAALTD++TCTDGFEDVADGP+K DVC
Sbjct: 126 EIRGSLKQMRQLGAAGAGGVSFRFQMSNVQTWMSAALTDEDTCTDGFEDVADGPLKTDVC 185

Query: 188 AKAEKVKKHTSNALALVNSFVQKRIP 212
            +  KV K TSNALALVNS+V+K  P
Sbjct: 186 DRVGKVHKFTSNALALVNSYVEKGTP 206

BLAST of Carg26064 vs. TrEMBL
Match: tr|A0A2I4G1T4|A0A2I4G1T4_9ROSI (21 kDa protein-like OS=Juglans regia OX=51240 GN=LOC109003963 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.3e-64
Identity = 134/210 (63.81%), Postives = 153/210 (72.86%), Query Frame = 0

Query: 2   RPQSPLILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCYA 61
           +P   L LSL     L  LR +PA  D     T    TAN+T+FIR+SC  TLYPD+CY 
Sbjct: 4   QPPRFLFLSLCLATLLFGLRPIPAVGD-----TGPVTTANATDFIRNSCGTTLYPDLCYT 63

Query: 62  SLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTN 121
           SLSRYAN IQQ+PA L +VAI +SLA +RR+ASYVSN+S   DYG D    S LHDCFTN
Sbjct: 64  SLSRYANAIQQNPAQLAKVAIGVSLAKARRIASYVSNVSRHSDYGTDPLTVSVLHDCFTN 123

Query: 122 FDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMK 181
           F DAVDE+RGSL QMRQ+      SFRFQM NVQTWMSAALTD+ETCTDGFEDV DGP+K
Sbjct: 124 FGDAVDEMRGSLQQMRQLGAAGAESFRFQMGNVQTWMSAALTDEETCTDGFEDVKDGPVK 183

Query: 182 ADVCAKAEKVKKHTSNALALVNSFVQKRIP 212
            D+C +  KVKK TSNALALVNS+V K IP
Sbjct: 184 TDMCGRVAKVKKFTSNALALVNSYVSKGIP 208

BLAST of Carg26064 vs. TrEMBL
Match: tr|A0A2C9UHG3|A0A2C9UHG3_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_15G132300 PE=4 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 9.6e-64
Identity = 133/212 (62.74%), Postives = 161/212 (75.94%), Query Frame = 0

Query: 1   MRPQSPLILSLLFVATLCLLRQVPAAADAADAGTSVNATANSTEFIRSSCVITLYPDVCY 60
           ++ Q PL+LSLLF   L  L+   A A    A  S     NST++IRSSCV TLYP++C+
Sbjct: 5   LQTQRPLLLSLLFATLLLHLQSFAAVASHGPASQS-----NSTDYIRSSCVATLYPEICF 64

Query: 61  ASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT 120
            SLSRYA+ +QQ+PA L RVAI +SL+ +RRM +YVSNLS   DYG+DHRAA+ALHDCF+
Sbjct: 65  TSLSRYASAVQQNPARLARVAIGVSLSRARRMTAYVSNLSRQADYGSDHRAAAALHDCFS 124

Query: 121 NFDDAVDEIRGSLNQMRQINDV--DVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADG 180
           NF DAVDEIRGSL QMRQ+        +FRFQMSNVQTWMSAALTD+ETCTDGFEDV +G
Sbjct: 125 NFGDAVDEIRGSLKQMRQLGAAGSSAEAFRFQMSNVQTWMSAALTDEETCTDGFEDVPEG 184

Query: 181 PMKADVCAKAEKVKKHTSNALALVNSFVQKRI 211
           P+K++VC +A  VKK TSNALALVNS+  K I
Sbjct: 185 PVKSEVCERAADVKKFTSNALALVNSYAAKGI 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022962658.13.5e-11099.0521 kDa protein-like [Cucurbita moschata][more]
XP_022990704.12.8e-10797.6421 kDa protein-like [Cucurbita maxima][more]
XP_023532575.11.8e-10697.1621 kDa protein-like [Cucurbita pepo subsp. pepo][more]
XP_008467116.19.7e-9286.26PREDICTED: 21 kDa protein [Cucumis melo][more]
XP_004150119.11.7e-9186.26PREDICTED: 21 kDa protein-like [Cucumis sativus] >KGN51480.1 hypothetical protei... [more]
Match NameE-valueIdentityDescription
AT1G23205.16.8e-4751.10Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT2G01610.17.8e-4346.82Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G14890.11.3e-4252.35Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G70720.19.5e-4150.57Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.14.9e-2940.33Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
Match NameE-valueIdentityDescription
sp|P17407|21KD_DAUCA1.8e-2839.5021 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1[more]
sp|Q9SB37|PMEI7_ARATH1.4e-2536.59Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1[more]
sp|Q9SI74|PMI10_ARATH5.9e-2438.27Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1[more]
sp|Q9STY5|PMI11_ARATH1.6e-2135.78Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1[more]
sp|Q9SI72|PMEI9_ARATH1.8e-2039.78Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3CSS6|A0A1S3CSS6_CUCME6.4e-9286.2621 kDa protein OS=Cucumis melo OX=3656 GN=LOC103504548 PE=4 SV=1[more]
tr|A0A0A0KPD9|A0A0A0KPD9_CUCSA1.1e-9186.26Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G567800 PE=4 SV=1[more]
tr|A0A2N9ETL4|A0A2N9ETL4_FAGSY1.1e-6464.56Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6015 PE=4 SV=1[more]
tr|A0A2I4G1T4|A0A2I4G1T4_9ROSI3.3e-6463.8121 kDa protein-like OS=Juglans regia OX=51240 GN=LOC109003963 PE=4 SV=1[more]
tr|A0A2C9UHG3|A0A2C9UHG3_MANES9.6e-6462.74Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_15G132300 PE=4 SV=... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR035513Invertase/methylesterase_inhib
IPR006501Pectinesterase_inhib_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26064-RACarg26064-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 41..201
e-value: 6.7E-43
score: 158.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 45..201
e-value: 1.9E-38
score: 132.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 11..203
e-value: 3.0E-31
score: 106.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3DG3DSA:1.20.140.40coord: 42..206
e-value: 3.2E-25
score: 90.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILYSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 44..205
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 6..206
NoneNo IPR availablePANTHERPTHR31080:SF10PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR DOMAIN-CONTAINING PROTEINcoord: 6..206
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 48..205
e-value: 3.44993E-44
score: 146.044

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg26064Carg23720Silver-seed gourdcarcarB440