Cp4.1LG01g10160 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG01g10160
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	GRAM domain-containing protein / ABA-responsive protein-related
Location	Cp4.1LG01 : 6881104 .. 6882166 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGATCTCTCTCTGTCCCGTGAAAAAGCTTGACAACTCTAGTTCAAATTCGATGTTTAGACATCTTAGAAGAAAAGTTGACAGATTCGTTAATGGAGTCTTTGAACGTGGTAAATTGCAATGTTTTTTTTTTTTTTTCCCTCTTGATTTGAGAGATCTCTCCCTAAATTTTACTTTCGCTACTACATGATTATGATGATGGATTGGATCATCCTCGAAATTTCCATTTCTAGACATGTACGAATGGCAAAATCCCCCCTCCCCACAATTCAATTGACAATTGTTATGCGTTCATCTAAGCATGATTCAACCAGTTGCTTATACGTTCATCTAAGCATAATTCAACCGGTTAAATAAGAAAAGAAGGATCACAAAGCTCATAGAACGCTAAAACCAAAAACTAACGCACTCTTGTTTGTTTCTCTATAAACAAAGATTGGCTAACGTATAACTCAAAAACCAAACCCGATTCAAATGATTACTTGCTTGATGGTTACAACGGAATGATGTCGTGCAGTTAAACTGTCGCCGAACATCACCGAGAATTTGAAGGGAAAGCTGTGGTCTGGGGCAAGACTTGTTCAGTTTGGAGGAAATGAGAAGATCTTTCAGAAAATGTTCAATCTCAGCCAAGGAGAGAAGTTACTAAGTTGTTCACGCTGTTATTTGTCAACCACAGCAGGTCCCGTAGCAGGCCTCCTCTTTATCTCCACCCATGTGGTTGCCTTCTGCAGTGACAGACCAATCGTCATCTCTTCTTCAGAAGGAGAACTGGGCAGATTGAACTACAAAGTAATATTATACAACATCCTTCAACATTCACATTAGACTCTCCCTCAATCCCTGTGGTTGCTAACAAGCTTTTTGTACTGTGCTTCATTCATCAGGTTATGATCCCATTGAAGAAGATGAAGAGAGTTAACCAAATAGAGCATATAAACAATCCATCAAAGAAGTACATTCAAGTGGTGACTGTGGATGATTTTGATTTCTGGTTTATGGGTTTTCTGAATTATGAGAAAGCATTCAAGTTCCTCCAAAAGAAGGTTTCTAGAGATTGA

mRNA sequence

ATGAAGATCTCTCTCTGTCCCGTGAAAAAGCTTGACAACTCTAGTTCAAATTCGATGTTTAGACATCTTAGAAGAAAAGTTGACAGATTCGTTAATGGAGTCTTTGAACGTGTTAAACTGTCGCCGAACATCACCGAGAATTTGAAGGGAAAGCTGTGGTCTGGGGCAAGACTTGTTCAGTTTGGAGGAAATGAGAAGATCTTTCAGAAAATGTTCAATCTCAGCCAAGGAGAGAAGTTACTAAGTTGTTCACGCTGTTATTTGTCAACCACAGCAGGTCCCGTAGCAGGCCTCCTCTTTATCTCCACCCATGTGGTTGCCTTCTGCAGTGACAGACCAATCGTCATCTCTTCTTCAGAAGGAGAACTGGGCAGATTGAACTACAAAGTTATGATCCCATTGAAGAAGATGAAGAGAGTTAACCAAATAGAGCATATAAACAATCCATCAAAGAAGTACATTCAAGTGGTGACTGTGGATGATTTTGATTTCTGGTTTATGGGTTTTCTGAATTATGAGAAAGCATTCAAGTTCCTCCAAAAGAAGGTTTCTAGAGATTGA

Coding sequence (CDS)

ATGAAGATCTCTCTCTGTCCCGTGAAAAAGCTTGACAACTCTAGTTCAAATTCGATGTTTAGACATCTTAGAAGAAAAGTTGACAGATTCGTTAATGGAGTCTTTGAACGTGTTAAACTGTCGCCGAACATCACCGAGAATTTGAAGGGAAAGCTGTGGTCTGGGGCAAGACTTGTTCAGTTTGGAGGAAATGAGAAGATCTTTCAGAAAATGTTCAATCTCAGCCAAGGAGAGAAGTTACTAAGTTGTTCACGCTGTTATTTGTCAACCACAGCAGGTCCCGTAGCAGGCCTCCTCTTTATCTCCACCCATGTGGTTGCCTTCTGCAGTGACAGACCAATCGTCATCTCTTCTTCAGAAGGAGAACTGGGCAGATTGAACTACAAAGTTATGATCCCATTGAAGAAGATGAAGAGAGTTAACCAAATAGAGCATATAAACAATCCATCAAAGAAGTACATTCAAGTGGTGACTGTGGATGATTTTGATTTCTGGTTTATGGGTTTTCTGAATTATGAGAAAGCATTCAAGTTCCTCCAAAAGAAGGTTTCTAGAGATTGA

Protein sequence

MKISLCPVKKLDNSSSNSMFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD

Homology

[Close]

BLAST of Cp4.1LG01g10160 vs. Swiss-Prot
Match: GEML4_ARATH (GEM-like protein 4 OS=Arabidopsis thaliana GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 6.0e-49
Identity = 95/176 (53.98%), Postives = 133/176 (75.57%), Query Frame = 1


Query: 9   KKLDNSSSNSMFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIF 68
           KK + S+  S+ +  R+K D F NGV ++ K+ P +TE +K KL  GAR++Q GG EKIF
Sbjct: 40  KKSEQSNVKSILK--RKKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIF 99

Query: 69  QKMFNLSQGEKLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNY 128
           +++F +S+GEKL    +CYLSTTAGP+AGLLFIS+  +AFCS+R I + S +G++ R++Y
Sbjct: 100 KRLFRVSEGEKLFKMYQCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHY 159

Query: 129 KVMIPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 185
           KV IPL K+ RVNQ ++   PS+KY++VVTVD FDFWFMGFL+Y+KAF  L+K +S
Sbjct: 160 KVSIPLCKIDRVNQSQNTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALS 213

[Close]

BLAST of Cp4.1LG01g10160 vs. Swiss-Prot
Match: GEML8_ARATH (Putative GEM-like protein 8 OS=Arabidopsis thaliana GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 5.6e-47
Identity = 92/167 (55.09%), Postives = 126/167 (75.45%), Query Frame = 1


Query: 18  SMFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQG 77
           SM R  ++K D F NGV ++ KL P +TE +K KL  GAR++Q GG EKI++++F +S  
Sbjct: 50  SMLR--KKKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDE 109

Query: 78  EKLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKM 137
           EKL    +CYLSTTAGP+AGLLFIS+  +AFCS+R I ++S +GEL R++YKV IPL K+
Sbjct: 110 EKLFKAYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKI 169

Query: 138 KRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 185
             VNQ ++   PS+KY++VVTVD FDFWFMGFL+Y+KAF  L++ +S
Sbjct: 170 NGVNQSQNTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALS 214

[Close]

BLAST of Cp4.1LG01g10160 vs. Swiss-Prot
Match: GEML6_ARATH (GEM-like protein 6 OS=Arabidopsis thaliana GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 1.4e-45
Identity = 86/169 (50.89%), Postives = 125/169 (73.96%), Query Frame = 1


Query: 18  SMFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQG 77
           SM R  ++K D F NG  ++ KL P +TE +K KL  GA+++Q GG EKI++++F +   
Sbjct: 50  SMLR--KKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQ 109

Query: 78  EKLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKM 137
           EKL    +CYLSTTAGP+AGLLFIS+  +AFCS+R I ++S +G L R++YKV IPL K+
Sbjct: 110 EKLFKAYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKI 169

Query: 138 KRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 187
             VNQ ++   PS+KY+++VT+D+FDFWFMGF++Y+KAF  L+K ++ D
Sbjct: 170 NGVNQSQNTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALNND 216

[Close]

BLAST of Cp4.1LG01g10160 vs. Swiss-Prot
Match: GEML7_ARATH (GEM-like protein 7 OS=Arabidopsis thaliana GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.5e-44
Identity = 84/179 (46.93%), Postives = 128/179 (71.51%), Query Frame = 1


Query: 8   VKKLDNSSSNSMFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKI 67
           + KL   +S+ +    ++K D F NG  ++ KL P +TE +K KL  GA+++Q GG EKI
Sbjct: 30  INKLQIPTSSKVSMLQKKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKI 89

Query: 68  FQKMFNLSQGEKLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLN 127
           ++++F +   EKL    +CYLSTT G +AGLLFIS+  +AFCS+R I ++S +G+L R++
Sbjct: 90  YKRLFKVCDKEKLFKAYQCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVH 149

Query: 128 YKVMIPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 187
           YKV IPL K+  VNQ ++   PS++Y++VVTVD++DFWFMGF++Y+KAF  L+K ++ D
Sbjct: 150 YKVSIPLCKINGVNQSQNTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALNED 208

[Close]

BLAST of Cp4.1LG01g10160 vs. Swiss-Prot
Match: GEML5_ARATH (GEM-like protein 5 OS=Arabidopsis thaliana GN=At5g13200 PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 2.8e-30
Identity = 67/166 (40.36%), Postives = 96/166 (57.83%), Query Frame = 1


Query: 19  MFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 78
           MF    RK +     ++  +K  P+++E   GK+   A+ +  GG E +F+++F     E
Sbjct: 96  MFHTWSRKAETVARNLWHNLKTGPSMSETAWGKVNLTAKAITKGGFESLFRQIFGTEPNE 155

Query: 79  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMK 138
            L     CYLSTT GPVAG +++S   VAFCSDRP+  ++  G+     Y+V++PL  + 
Sbjct: 156 TLKKTFACYLSTTTGPVAGTVYLSNARVAFCSDRPLYFTAPSGQESWSYYRVVVPLANVA 215

Query: 139 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 185
            VN +     P +KYIQ+ TVD  DFWFMGF+NYEKA   L   VS
Sbjct: 216 TVNPVVVKETPPEKYIQLTTVDGHDFWFMGFVNYEKATHHLLTSVS 261

[Close]

BLAST of Cp4.1LG01g10160 vs. TrEMBL
Match: A0A0A0KPM0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G577990 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 7.9e-64
Identity = 117/168 (69.64%), Postives = 141/168 (83.93%), Query Frame = 1


Query: 19  MFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 78
           M R L R+   FVNG+ E VKLSPNI + +KGKLW G +L+QFGG+E IF KMFNL  G+
Sbjct: 1   MIRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGD 60

Query: 79  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMK 138
           KLL+ + CYLSTTAGP+AGL+F+STHVVAFCSDRPI+ISS  GE+G++ YKVMIP+ K+K
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120

Query: 139 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 187
           RVNQ  ++NNP+KKYIQVVTVDDFDFWFMGFLN+EKAFKF+Q  VS D
Sbjct: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVD 168

[Close]

BLAST of Cp4.1LG01g10160 vs. TrEMBL
Match: D7TY17_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_10s0071g00320 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 6.2e-53
Identity = 101/163 (61.96%), Postives = 134/163 (82.21%), Query Frame = 1


Query: 23  LRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLS 82
           L +K D FVNGV E V+L+PNI+E +KGKL  GAR++Q GG EKIF+++F + +GEKLL 
Sbjct: 55  LGKKADSFVNGVREHVRLAPNISETVKGKLSLGARILQVGGVEKIFKRLFRVREGEKLLK 114

Query: 83  CSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMKRVNQ 142
            S+CYLSTTAGP+AGLLFIST  +AFCS+R I  SS+ GEL R++YKV+IPL+K+K  NQ
Sbjct: 115 ASQCYLSTTAGPIAGLLFISTDKIAFCSERAIKFSSANGELVRIHYKVLIPLRKIKIANQ 174

Query: 143 IEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSR 186
            E+   PS+KYI++VT D+F+FWFMGFLNY+KAFK+LQ+ +S+
Sbjct: 175 SENTKKPSQKYIEIVTTDNFEFWFMGFLNYQKAFKYLQQALSQ 217

[Close]

BLAST of Cp4.1LG01g10160 vs. TrEMBL
Match: A0A061E267_THECC (GRAM domain-containing protein / ABA-responsive protein-related OS=Theobroma cacao GN=TCM_007675 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 5.3e-52
Identity = 101/163 (61.96%), Postives = 132/163 (80.98%), Query Frame = 1


Query: 23  LRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLS 82
           L +K D F +GV E V+L P ITE +KGKL  GAR++Q GG EKIF+++F++ +GEKLL 
Sbjct: 63  LGKKADTFAHGVREHVRLGPKITETVKGKLSLGARILQVGGVEKIFKRLFSVREGEKLLK 122

Query: 83  CSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMKRVNQ 142
             +CYLSTTAGP+AGLLF+S+  VAFCS+R I I S +GEL R++YKV+IPL+K+K VNQ
Sbjct: 123 ACQCYLSTTAGPIAGLLFVSSEKVAFCSERSIKIPSPKGELPRVHYKVLIPLEKIKGVNQ 182

Query: 143 IEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSR 186
            E+I  PS+KYI++VTVD FDFWFMGFLNY KAFK+LQ+ +S+
Sbjct: 183 SENIKKPSQKYIEMVTVDGFDFWFMGFLNYRKAFKYLQQAISQ 225

[Close]

BLAST of Cp4.1LG01g10160 vs. TrEMBL
Match: V4TC71_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10032699mg PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 2.0e-51
Identity = 98/160 (61.25%), Postives = 128/160 (80.00%), Query Frame = 1


Query: 25  RKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCS 84
           +K DRF  GV E V+L P ITE +KGKL  GAR++Q GG  K+F+K+FN+++GE+LL   
Sbjct: 61  KKADRFATGVREHVRLGPKITEIVKGKLSLGARIIQVGGMGKVFKKLFNVAEGERLLKTC 120

Query: 85  RCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMKRVNQIE 144
           +CYLSTTAGP+AGLLFIST  +AFCS+R I I S  GE  R++YKV+IP+KK+KRVNQ E
Sbjct: 121 QCYLSTTAGPIAGLLFISTEKIAFCSERSIKIPSPNGESARIHYKVVIPVKKIKRVNQSE 180

Query: 145 HINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 185
           ++  PS+KYI++VTVD+FDFWFMGFLNY+KA   LQ+ +S
Sbjct: 181 NVYKPSQKYIEIVTVDNFDFWFMGFLNYQKALNHLQQAIS 220

[Close]

BLAST of Cp4.1LG01g10160 vs. TrEMBL
Match: A0A067G363_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g027631mg PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 2.0e-51
Identity = 98/160 (61.25%), Postives = 128/160 (80.00%), Query Frame = 1


Query: 25  RKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCS 84
           +K DRF  GV E V+L P ITE +KGKL  GAR++Q GG  K+F+K+FN+++GE+LL   
Sbjct: 61  KKADRFATGVREHVRLGPKITEIVKGKLSLGARIIQVGGMGKVFKKLFNVAEGERLLKTC 120

Query: 85  RCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMKRVNQIE 144
           +CYLSTTAGP+AGLLFIST  +AFCS+R I I S  GE  R++YKV+IP+KK+KRVNQ E
Sbjct: 121 QCYLSTTAGPIAGLLFISTEKIAFCSERSIKIPSPNGESARIHYKVVIPVKKIKRVNQSE 180

Query: 145 HINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 185
           ++  PS+KYI++VTVD+FDFWFMGFLNY+KA   LQ+ +S
Sbjct: 181 NVYKPSQKYIEIVTVDNFDFWFMGFLNYQKALNHLQQAIS 220

[Close]

BLAST of Cp4.1LG01g10160 vs. TAIR10
Match: AT5G08350.1 (AT5G08350.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 195.3 bits (495), Expect = 3.4e-50
Identity = 95/176 (53.98%), Postives = 133/176 (75.57%), Query Frame = 1


Query: 9   KKLDNSSSNSMFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIF 68
           KK + S+  S+ +  R+K D F NGV ++ K+ P +TE +K KL  GAR++Q GG EKIF
Sbjct: 40  KKSEQSNVKSILK--RKKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIF 99

Query: 69  QKMFNLSQGEKLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNY 128
           +++F +S+GEKL    +CYLSTTAGP+AGLLFIS+  +AFCS+R I + S +G++ R++Y
Sbjct: 100 KRLFRVSEGEKLFKMYQCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHY 159

Query: 129 KVMIPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 185
           KV IPL K+ RVNQ ++   PS+KY++VVTVD FDFWFMGFL+Y+KAF  L+K +S
Sbjct: 160 KVSIPLCKIDRVNQSQNTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALS 213

[Close]

BLAST of Cp4.1LG01g10160 vs. TAIR10
Match: AT5G23370.1 (AT5G23370.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 188.7 bits (478), Expect = 3.2e-48
Identity = 92/167 (55.09%), Postives = 126/167 (75.45%), Query Frame = 1


Query: 18  SMFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQG 77
           SM R  ++K D F NGV ++ KL P +TE +K KL  GAR++Q GG EKI++++F +S  
Sbjct: 50  SMLR--KKKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDE 109

Query: 78  EKLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKM 137
           EKL    +CYLSTTAGP+AGLLFIS+  +AFCS+R I ++S +GEL R++YKV IPL K+
Sbjct: 110 EKLFKAYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKI 169

Query: 138 KRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 185
             VNQ ++   PS+KY++VVTVD FDFWFMGFL+Y+KAF  L++ +S
Sbjct: 170 NGVNQSQNTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALS 214

[Close]

BLAST of Cp4.1LG01g10160 vs. TAIR10
Match: AT5G23350.1 (AT5G23350.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 184.1 bits (466), Expect = 7.8e-47
Identity = 86/169 (50.89%), Postives = 125/169 (73.96%), Query Frame = 1


Query: 18  SMFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQG 77
           SM R  ++K D F NG  ++ KL P +TE +K KL  GA+++Q GG EKI++++F +   
Sbjct: 112 SMLR--KKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQ 171

Query: 78  EKLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKM 137
           EKL    +CYLSTTAGP+AGLLFIS+  +AFCS+R I ++S +G L R++YKV IPL K+
Sbjct: 172 EKLFKAYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKI 231

Query: 138 KRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 187
             VNQ ++   PS+KY+++VT+D+FDFWFMGF++Y+KAF  L+K ++ D
Sbjct: 232 NGVNQSQNTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALNND 278

[Close]

BLAST of Cp4.1LG01g10160 vs. TAIR10
Match: AT5G23360.1 (AT5G23360.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 180.6 bits (457), Expect = 8.6e-46
Identity = 84/179 (46.93%), Postives = 128/179 (71.51%), Query Frame = 1


Query: 8   VKKLDNSSSNSMFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKI 67
           + KL   +S+ +    ++K D F NG  ++ KL P +TE +K KL  GA+++Q GG EKI
Sbjct: 30  INKLQIPTSSKVSMLQKKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKI 89

Query: 68  FQKMFNLSQGEKLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLN 127
           ++++F +   EKL    +CYLSTT G +AGLLFIS+  +AFCS+R I ++S +G+L R++
Sbjct: 90  YKRLFKVCDKEKLFKAYQCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVH 149

Query: 128 YKVMIPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 187
           YKV IPL K+  VNQ ++   PS++Y++VVTVD++DFWFMGF++Y+KAF  L+K ++ D
Sbjct: 150 YKVSIPLCKINGVNQSQNTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALNED 208

[Close]

BLAST of Cp4.1LG01g10160 vs. TAIR10
Match: AT5G13200.1 (AT5G13200.1 GRAM domain family protein)

HSP 1 Score: 133.3 bits (334), Expect = 1.6e-31
Identity = 67/166 (40.36%), Postives = 96/166 (57.83%), Query Frame = 1


Query: 19  MFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 78
           MF    RK +     ++  +K  P+++E   GK+   A+ +  GG E +F+++F     E
Sbjct: 96  MFHTWSRKAETVARNLWHNLKTGPSMSETAWGKVNLTAKAITKGGFESLFRQIFGTEPNE 155

Query: 79  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMK 138
            L     CYLSTT GPVAG +++S   VAFCSDRP+  ++  G+     Y+V++PL  + 
Sbjct: 156 TLKKTFACYLSTTTGPVAGTVYLSNARVAFCSDRPLYFTAPSGQESWSYYRVVVPLANVA 215

Query: 139 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 185
            VN +     P +KYIQ+ TVD  DFWFMGF+NYEKA   L   VS
Sbjct: 216 TVNPVVVKETPPEKYIQLTTVDGHDFWFMGFVNYEKATHHLLTSVS 261

[Close]

BLAST of Cp4.1LG01g10160 vs. NCBI nr
Match: gi|659090209|ref|XP_008445893.1| (PREDICTED: GEM-like protein 4 [Cucumis melo])

HSP 1 Score: 257.3 bits (656), Expect = 2.1e-65
Identity = 122/168 (72.62%), Postives = 142/168 (84.52%), Query Frame = 1


Query: 19  MFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 78
           M R L RK   FVNG+ E VKLSPNI + +KGKLW G +LVQFGG+EKIF KMFNL QG+
Sbjct: 1   MIRELGRKGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFHKMFNLEQGD 60

Query: 79  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMK 138
           KLL+ + CYLSTTAGP+AGL+F+STHVVAFCSDRPI+ISS  GE+G++ YKVMIP+ K+K
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEVGKVFYKVMIPVNKVK 120

Query: 139 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 187
           RVNQ  + NNP+KKYIQVVTVDDFDFWFMGFLNYEK FKF+QK VS D
Sbjct: 121 RVNQRNNENNPAKKYIQVVTVDDFDFWFMGFLNYEKTFKFIQKGVSMD 168

[Close]

BLAST of Cp4.1LG01g10160 vs. NCBI nr
Match: gi|449458799|ref|XP_004147134.1| (PREDICTED: GEM-like protein 4 [Cucumis sativus])

HSP 1 Score: 251.5 bits (641), Expect = 1.1e-63
Identity = 117/168 (69.64%), Postives = 141/168 (83.93%), Query Frame = 1


Query: 19  MFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 78
           M R L R+   FVNG+ E VKLSPNI + +KGKLW G +L+QFGG+E IF KMFNL  G+
Sbjct: 1   MIRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGD 60

Query: 79  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMK 138
           KLL+ + CYLSTTAGP+AGL+F+STHVVAFCSDRPI+ISS  GE+G++ YKVMIP+ K+K
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120

Query: 139 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 187
           RVNQ  ++NNP+KKYIQVVTVDDFDFWFMGFLN+EKAFKF+Q  VS D
Sbjct: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVD 168

[Close]

BLAST of Cp4.1LG01g10160 vs. NCBI nr
Match: gi|225463675|ref|XP_002275935.1| (PREDICTED: GEM-like protein 4 [Vitis vinifera])

HSP 1 Score: 215.3 bits (547), Expect = 9.0e-53
Identity = 101/163 (61.96%), Postives = 134/163 (82.21%), Query Frame = 1


Query: 23  LRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLS 82
           L +K D FVNGV E V+L+PNI+E +KGKL  GAR++Q GG EKIF+++F + +GEKLL 
Sbjct: 55  LGKKADSFVNGVREHVRLAPNISETVKGKLSLGARILQVGGVEKIFKRLFRVREGEKLLK 114

Query: 83  CSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMKRVNQ 142
            S+CYLSTTAGP+AGLLFIST  +AFCS+R I  SS+ GEL R++YKV+IPL+K+K  NQ
Sbjct: 115 ASQCYLSTTAGPIAGLLFISTDKIAFCSERAIKFSSANGELVRIHYKVLIPLRKIKIANQ 174

Query: 143 IEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSR 186
            E+   PS+KYI++VT D+F+FWFMGFLNY+KAFK+LQ+ +S+
Sbjct: 175 SENTKKPSQKYIEIVTTDNFEFWFMGFLNYQKAFKYLQQALSQ 217

[Close]

BLAST of Cp4.1LG01g10160 vs. NCBI nr
Match: gi|590689422|ref|XP_007043224.1| (GRAM domain-containing protein / ABA-responsive protein-related [Theobroma cacao])

HSP 1 Score: 212.2 bits (539), Expect = 7.6e-52
Identity = 101/163 (61.96%), Postives = 132/163 (80.98%), Query Frame = 1


Query: 23  LRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLS 82
           L +K D F +GV E V+L P ITE +KGKL  GAR++Q GG EKIF+++F++ +GEKLL 
Sbjct: 63  LGKKADTFAHGVREHVRLGPKITETVKGKLSLGARILQVGGVEKIFKRLFSVREGEKLLK 122

Query: 83  CSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMKRVNQ 142
             +CYLSTTAGP+AGLLF+S+  VAFCS+R I I S +GEL R++YKV+IPL+K+K VNQ
Sbjct: 123 ACQCYLSTTAGPIAGLLFVSSEKVAFCSERSIKIPSPKGELPRVHYKVLIPLEKIKGVNQ 182

Query: 143 IEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSR 186
            E+I  PS+KYI++VTVD FDFWFMGFLNY KAFK+LQ+ +S+
Sbjct: 183 SENIKKPSQKYIEMVTVDGFDFWFMGFLNYRKAFKYLQQAISQ 225

[Close]

BLAST of Cp4.1LG01g10160 vs. NCBI nr
Match: gi|645228617|ref|XP_008221081.1| (PREDICTED: GEM-like protein 4 [Prunus mume])

HSP 1 Score: 211.5 bits (537), Expect = 1.3e-51
Identity = 102/182 (56.04%), Postives = 136/182 (74.73%), Query Frame = 1


Query: 7   PVKKLDNSSSNSMFRHLRR---KVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGG 66
           P K       NS+ + + +   K D F +GV E VKL P ITE +KGKL  GA+++Q GG
Sbjct: 45  PSKLRPTGRVNSVLKRMNKLGKKADSFAHGVKEHVKLGPKITETVKGKLSLGAKIIQVGG 104

Query: 67  NEKIFQKMFNLSQGEKLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGEL 126
            EK+F+  F++  GEKLL  S+CYLSTTAGP+AGLLFIST  +AFCS+R I +SSS GEL
Sbjct: 105 LEKVFKHAFSVRSGEKLLKASQCYLSTTAGPIAGLLFISTDKIAFCSERSIKLSSSNGEL 164

Query: 127 GRLNYKVMIPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKV 186
            RL+YKV+IP+ K+KRVNQ E++  PS+KYI++VTVD+FDFWFMG LNY+K  K+L++ +
Sbjct: 165 IRLHYKVVIPVNKIKRVNQSENVKKPSQKYIEIVTVDNFDFWFMGILNYQKTLKYLEEAI 224

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g10160 vs. Swiss-Prot


Match Name	E-value	Identity	Description
GEML4_ARATH	6.0e-49	53.98	GEM-like protein 4 OS=Arabidopsis thaliana GN=At5g08350 PE=2 SV=1	[more]
GEML8_ARATH	5.6e-47	55.09	Putative GEM-like protein 8 OS=Arabidopsis thaliana GN=At5g23370 PE=3 SV=1	[more]
GEML6_ARATH	1.4e-45	50.89	GEM-like protein 6 OS=Arabidopsis thaliana GN=At5g23350 PE=2 SV=2	[more]
GEML7_ARATH	1.5e-44	46.93	GEM-like protein 7 OS=Arabidopsis thaliana GN=At5g23360 PE=2 SV=1	[more]
GEML5_ARATH	2.8e-30	40.36	GEM-like protein 5 OS=Arabidopsis thaliana GN=At5g13200 PE=1 SV=1	[more]

BLAST of Cp4.1LG01g10160 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPM0_CUCSA	7.9e-64	69.64	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G577990 PE=4 SV=1	[more]
D7TY17_VITVI	6.2e-53	61.96	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_10s0071g00320 PE=4 SV=...	[more]
A0A061E267_THECC	5.3e-52	61.96	GRAM domain-containing protein / ABA-responsive protein-related OS=Theobroma cac...	[more]
V4TC71_9ROSI	2.0e-51	61.25	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10032699mg PE=4 SV=1	[more]
A0A067G363_CITSI	2.0e-51	61.25	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g027631mg PE=4 SV=1	[more]

BLAST of Cp4.1LG01g10160 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G08350.1	3.4e-50	53.98	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23370.1	3.2e-48	55.09	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23350.1	7.8e-47	50.89	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23360.1	8.6e-46	46.93	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G13200.1	1.6e-31	40.36	GRAM domain family protein	[more]

BLAST of Cp4.1LG01g10160 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659090209\|ref\|XP_008445893.1\|	2.1e-65	72.62	PREDICTED: GEM-like protein 4 [Cucumis melo]	[more]
gi\|449458799\|ref\|XP_004147134.1\|	1.1e-63	69.64	PREDICTED: GEM-like protein 4 [Cucumis sativus]	[more]
gi\|225463675\|ref\|XP_002275935.1\|	9.0e-53	61.96	PREDICTED: GEM-like protein 4 [Vitis vinifera]	[more]
gi\|590689422\|ref\|XP_007043224.1\|	7.6e-52	61.96	GRAM domain-containing protein / ABA-responsive protein-related [Theobroma cacao...	[more]
gi\|645228617\|ref\|XP_008221081.1\|	1.3e-51	56.04	PREDICTED: GEM-like protein 4 [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR004182	GRAM

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g10160.1	Cp4.1LG01g10160.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004182	GRAM domain	PFAM	PF02893	GRAM	coord: 67..143 score: 2.6
IPR004182	GRAM domain	SMART	SM00568	gram2001c	coord: 66..144 score: 9.2
None	No IPR available	PANTHER	PTHR31969	FAMILY NOT NAMED	coord: 14..185 score: 8.5
None	No IPR available	PANTHER	PTHR31969:SF1	GEM-LIKE PROTEIN 4-RELATED	coord: 14..185 score: 8.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG01g10160	Cucsa.280920	Cucumber (Gy14) v1	cgycpeB0736
Cp4.1LG01g10160	CmaCh18G008130	Cucurbita maxima (Rimu)	cmacpeB429
Cp4.1LG01g10160	CmaCh04G012650	Cucurbita maxima (Rimu)	cmacpeB720
Cp4.1LG01g10160	CmoCh04G013360	Cucurbita moschata (Rifu)	cmocpeB673
Cp4.1LG01g10160	CmoCh18G008150	Cucurbita moschata (Rifu)	cmocpeB392
Cp4.1LG01g10160	Cla020045	Watermelon (97103) v1	cpewmB451
Cp4.1LG01g10160	Csa5G577990	Cucumber (Chinese Long) v2	cpecuB418
Cp4.1LG01g10160	ClCG02G014170	Watermelon (Charleston Gray)	cpewcgB376
Cp4.1LG01g10160	CSPI05G20270	Wild cucumber (PI 183967)	cpecpiB417
Cp4.1LG01g10160	Lsi04G007860	Bottle gourd (USVL1VR-Ls)	cpelsiB358
Cp4.1LG01g10160	MELO3C034197.2	Melon (DHL92) v3.6.1	cpemedB436
Cp4.1LG01g10160	MELO3C005069.2	Melon (DHL92) v3.6.1	cpemedB416
Cp4.1LG01g10160	CsaV3_5G029030	Cucumber (Chinese Long) v3	cpecucB0520
Cp4.1LG01g10160	Bhi07G000909	Wax gourd	cpewgoB0570
Cp4.1LG01g10160	CsGy5G019800	Cucumber (Gy14) v2	cgybcpeB628

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG01g10160	Cp4.1LG09g04850	Cucurbita pepo (Zucchini)	cpecpeB041

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG01g10160	Wax gourd	cpewgoB0556
Cp4.1LG01g10160	Cucurbita pepo (Zucchini)	cpecpeB380
Cp4.1LG01g10160	Cucurbita maxima (Rimu)	cmacpeB736
Cp4.1LG01g10160	Cucurbita moschata (Rifu)	cmocpeB690
Cp4.1LG01g10160	Bottle gourd (USVL1VR-Ls)	cpelsiB317
Cp4.1LG01g10160	Melon (DHL92) v3.5.1	cpemeB357
Cp4.1LG01g10160	Melon (DHL92) v3.5.1	cpemeB376
Cp4.1LG01g10160	Silver-seed gourd	carcpeB0671
Cp4.1LG01g10160	Silver-seed gourd	carcpeB1021
Cp4.1LG01g10160	Silver-seed gourd	carcpeB1177