CSPI05G20270 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI05G20270
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	GRAM domain-containing protein / ABA-responsive protein-related
Location	Chr5 : 21409405 .. 21410479 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCAGAGAGCTTGGAAGAAGAGGTTACAGCTTTGTTAATGGAATCCTTGAACATGGTAAACATAAATTGCTTTCTTCTTCTTTCCTGTCTGACTATGATCATGAAATGATGGATTGGATTGAATTGTCCTCTTTGTTTTCTAGAAACTTCTGTTTCTCGTCTTGTTTTCTATAAATCCAATGCATAATATCTCGAAATCCCTCCGTCGATCTAAACATGATTCAACTAGAGAAACCGAAAGCACTATTTATCTCTTCCCACGTAGATAGAGATTGGCCTTGATTCTTCCTGCATTGATAACAATGTATAAATATACTTGATTCGAGGGATTAAAAATGCTTGACGGTTGCAATGGAATGATGACATGCAGTTAAACTGTCGCCGAACATAGCCAAAACGGTGAAGGGGAAGCTGTGGTTGGGGACAAAACTTTTACAGTTTGGAGGAAGTGAGAATATATTCCACAAGATGTTCAATCTCGAACACGGAGACAAGTTATTGAATTCTGCACATTGTTATTTGTCAACAACAGCAGGTCCTATAGCAGGTCTCATCTTTGTTTCCACCCATGTAGTTGCTTTCTGCAGTGACAGACCAATCATCATTTCTTCTCCACATGGAGAAATTGGCAAAATATTTTACAAGGTAATTAATATTTCCTTTCTTACAACATTTACATTTGACAATTGCCCTCACTATGTCTCTTTCTTCCTGGTTGCTAACCAAGTTTTTGTTTTCTATAATATTGTTTATCCCATCAGGTTATGATCCCCGTGAACAAAGTGAAGAGAGTTAACCAAAGAAACAATGTAAACAATCCAGCAAAGAAATACATTCAAGTGGTGACTGTGGATGATTTTGACTTCTGGTTTATGGGTTTTTTGAATCATGAGAAAGCTTTTAAGTTCATACAAAATGGTGTCTCTGTGGATTCAGAAGATTGGCCTGAAGAGGCCCAAAGAAAGTGTTGTCCAGGAAAGTTCAAGACATTGTAAAGGTTATGACAACTCTTAACTCATACCAGGGGATCAGATATTTTTAACTCCGTTTAGTTTGATCATCTATGAAGCAC

mRNA sequence

ATGATCAGAGAGCTTGGAAGAAGAGGTTACAGCTTTGTTAATGGAATCCTTGAACATGTTAAACTGTCGCCGAACATAGCCAAAACGGTGAAGGGGAAGCTGTGGTTGGGGACAAAACTTTTACAGTTTGGAGGAAGTGAGAATATATTCCACAAGATGTTCAATCTCGAACACGGAGACAAGTTATTGAATTCTGCACATTGTTATTTGTCAACAACAGCAGGTCCTATAGCAGGTCTCATCTTTGTTTCCACCCATGTAGTTGCTTTCTGCAGTGACAGACCAATCATCATTTCTTCTCCACATGGAGAAATTGGCAAAATATTTTACAAGGTTATGATCCCCGTGAACAAAGTGAAGAGAGTTAACCAAAGAAACAATGTAAACAATCCAGCAAAGAAATACATTCAAGTGGTGACTGTGGATGATTTTGACTTCTGGTTTATGGGTTTTTTGAATCATGAGAAAGCTTTTAAGTTCATACAAAATGGTGTCTCTGTGGATTCAGAAGATTGGCCTGAAGAGGCCCAAAGAAAGTGTTGTCCAGGAAAGTTCAAGACATTGTAA

Coding sequence (CDS)

ATGATCAGAGAGCTTGGAAGAAGAGGTTACAGCTTTGTTAATGGAATCCTTGAACATGTTAAACTGTCGCCGAACATAGCCAAAACGGTGAAGGGGAAGCTGTGGTTGGGGACAAAACTTTTACAGTTTGGAGGAAGTGAGAATATATTCCACAAGATGTTCAATCTCGAACACGGAGACAAGTTATTGAATTCTGCACATTGTTATTTGTCAACAACAGCAGGTCCTATAGCAGGTCTCATCTTTGTTTCCACCCATGTAGTTGCTTTCTGCAGTGACAGACCAATCATCATTTCTTCTCCACATGGAGAAATTGGCAAAATATTTTACAAGGTTATGATCCCCGTGAACAAAGTGAAGAGAGTTAACCAAAGAAACAATGTAAACAATCCAGCAAAGAAATACATTCAAGTGGTGACTGTGGATGATTTTGACTTCTGGTTTATGGGTTTTTTGAATCATGAGAAAGCTTTTAAGTTCATACAAAATGGTGTCTCTGTGGATTCAGAAGATTGGCCTGAAGAGGCCCAAAGAAAGTGTTGTCCAGGAAAGTTCAAGACATTGTAA

Homology

[Close]

BLAST of CSPI05G20270 vs. Swiss-Prot
Match: GEML4_ARATH (GEM-like protein 4 OS=Arabidopsis thaliana GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.8e-45
Identity = 83/164 (50.61%), Postives = 117/164 (71.34%), Query Frame = 1


Query: 12  FVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDKLLNSAHCYLS 71
           F NG+ +  K+ P + +TVK KL LG ++LQ GG E IF ++F +  G+KL     CYLS
Sbjct: 59  FTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYLS 118

Query: 72  TTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNNP 131
           TTAGPIAGL+F+S+  +AFCS+R I + SP G+I ++ YKV IP+ K+ RVNQ  N   P
Sbjct: 119 TTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQNTKKP 178

Query: 132 AKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSEDWPEE 176
           ++KY++VVTVD FDFWFMGFL+++KAF  ++  +S+  ED  E+
Sbjct: 179 SQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALSLSFEDNKEQ 222

[Close]

BLAST of CSPI05G20270 vs. Swiss-Prot
Match: GEML8_ARATH (Putative GEM-like protein 8 OS=Arabidopsis thaliana GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 3.5e-44
Identity = 79/161 (49.07%), Postives = 116/161 (72.05%), Query Frame = 1


Query: 7   RRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDKLLNSA 66
           ++  SF NG+ +  KL P + +TVK KL LG ++LQ GG E I+ ++F +   +KL  + 
Sbjct: 55  KKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAY 114

Query: 67  HCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRN 126
            CYLSTTAGPIAGL+F+S+  +AFCS+R I ++SP GE+ ++ YKV IP+ K+  VNQ  
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQ 174

Query: 127 NVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSV 168
           N   P++KY++VVTVD FDFWFMGFL+++KAF  ++  +S+
Sbjct: 175 NTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALSL 215

[Close]

BLAST of CSPI05G20270 vs. Swiss-Prot
Match: GEML6_ARATH (GEM-like protein 6 OS=Arabidopsis thaliana GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 173.7 bits (439), Expect = 1.9e-42
Identity = 76/160 (47.50%), Postives = 114/160 (71.25%), Query Frame = 1


Query: 11  SFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDKLLNSAHCYL 70
           SF NG  +  KL P + +TVK KL LG K+LQ GG E I+ ++F +   +KL  +  CYL
Sbjct: 59  SFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYL 118

Query: 71  STTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNN 130
           STTAGPIAGL+F+S+  +AFCS+R I ++SP G + ++ YKV IP+ K+  VNQ  N   
Sbjct: 119 STTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQNTKK 178

Query: 131 PAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSE 171
           P++KY+++VT+D+FDFWFMGF++++KAF  ++  ++ D +
Sbjct: 179 PSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALNNDEQ 218

[Close]

BLAST of CSPI05G20270 vs. Swiss-Prot
Match: GEML7_ARATH (GEM-like protein 7 OS=Arabidopsis thaliana GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 8.0e-41
Identity = 74/160 (46.25%), Postives = 113/160 (70.62%), Query Frame = 1


Query: 11  SFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDKLLNSAHCYL 70
           SF NG  +  KL P + +TVK KL LG K+LQ GG E I+ ++F +   +KL  +  CYL
Sbjct: 51  SFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQCYL 110

Query: 71  STTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNN 130
           STT G IAGL+F+S+  +AFCS+R I ++SP G++ ++ YKV IP+ K+  VNQ  N   
Sbjct: 111 STTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQNTKK 170

Query: 131 PAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSE 171
           P+++Y++VVTVD++DFWFMGF++++KAF  ++  ++ D +
Sbjct: 171 PSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALNEDEQ 210

[Close]

BLAST of CSPI05G20270 vs. Swiss-Prot
Match: GEM_ARATH (GLABRA2 expression modulator OS=Arabidopsis thaliana GN=GEM PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 7.5e-31
Identity = 63/165 (38.18%), Postives = 109/165 (66.06%), Query Frame = 1


Query: 2   IRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDK 61
           + E  ++  S      +H++ +P+ A    G++   TK+   GG E IF + F  +  ++
Sbjct: 129 VAEAAKKTESLAGNTWQHLRTAPSFADAAMGRIAQSTKVFAEGGYEKIFRQTFETDPEEQ 188

Query: 62  LLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGK-IFYKVMIPVNKVK 121
           LLNS  CYLST+AGP+ G++++S+  +A+CSD P  +S  +G+  +  +YKV+IP++++K
Sbjct: 189 LLNSFACYLSTSAGPVMGVLYISSAKLAYCSDNP--LSYKNGDQTEWSYYKVVIPLHQLK 248

Query: 122 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGV 166
            VN   ++ NPA+KYIQV++VD+ +FWFMGFLN++ A   +Q+ +
Sbjct: 249 AVNPSASIVNPAEKYIQVISVDNHEFWFMGFLNYDGAVTSLQDSL 291

[Close]

BLAST of CSPI05G20270 vs. TrEMBL
Match: A0A0A0KPM0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G577990 PE=4 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 4.5e-107
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 1


Query: 1   MIRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGD 60
           MIRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGD
Sbjct: 1   MIRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGD 60

Query: 61  KLLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
           KLLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSEDWPEEAQRKC 180
           RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSEDWPEEAQRKC
Sbjct: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSEDWPEEAQRKC 180

Query: 181 CPGKFKTL 189
           CPGKFKTL
Sbjct: 181 CPGKFKTL 188

[Close]

BLAST of CSPI05G20270 vs. TrEMBL
Match: M5Y243_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa026708mg PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.2e-51
Identity = 93/165 (56.36%), Postives = 130/165 (78.79%), Query Frame = 1


Query: 2   IRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDK 61
           + +LG++  SF +G+ EHV+L P I +TVKGKL LG K++Q GG E +F   F++  G+K
Sbjct: 57  MNKLGKKADSFAHGVKEHVRLGPKITETVKGKLSLGAKIIQVGGLEKVFKHAFSVRAGEK 116

Query: 62  LLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKR 121
           LL  + CYLSTTAGPIAGL+F+ST  +AFCS+R I +SSP+GE+ ++ YKV+IPVNK+KR
Sbjct: 117 LLKVSQCYLSTTAGPIAGLLFISTDKIAFCSERSIKLSSPNGELIRVHYKVVIPVNKIKR 176

Query: 122 VNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVS 167
           VNQ  NV  P++KYI++VTVD+FDFWFMGFLN++K  K+++  +S
Sbjct: 177 VNQSENVKKPSEKYIEIVTVDNFDFWFMGFLNYQKTLKYLEEAIS 221

[Close]

BLAST of CSPI05G20270 vs. TrEMBL
Match: A0A061E267_THECC (GRAM domain-containing protein / ABA-responsive protein-related OS=Theobroma cacao GN=TCM_007675 PE=4 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.6e-51
Identity = 95/169 (56.21%), Postives = 129/169 (76.33%), Query Frame = 1


Query: 5   LGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDKLLN 64
           LG++  +F +G+ EHV+L P I +TVKGKL LG ++LQ GG E IF ++F++  G+KLL 
Sbjct: 63  LGKKADTFAHGVREHVRLGPKITETVKGKLSLGARILQVGGVEKIFKRLFSVREGEKLLK 122

Query: 65  SAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQ 124
           +  CYLSTTAGPIAGL+FVS+  VAFCS+R I I SP GE+ ++ YKV+IP+ K+K VNQ
Sbjct: 123 ACQCYLSTTAGPIAGLLFVSSEKVAFCSERSIKIPSPKGELPRVHYKVLIPLEKIKGVNQ 182

Query: 125 RNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSEDWP 174
             N+  P++KYI++VTVD FDFWFMGFLN+ KAFK++Q  +S   +D P
Sbjct: 183 SENIKKPSQKYIEMVTVDGFDFWFMGFLNYRKAFKYLQQAISQRLDDVP 231

[Close]

BLAST of CSPI05G20270 vs. TrEMBL
Match: D7TY17_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_10s0071g00320 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 2.0e-51
Identity = 93/165 (56.36%), Postives = 131/165 (79.39%), Query Frame = 1


Query: 2   IRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDK 61
           + +LG++  SFVNG+ EHV+L+PNI++TVKGKL LG ++LQ GG E IF ++F +  G+K
Sbjct: 52  MNKLGKKADSFVNGVREHVRLAPNISETVKGKLSLGARILQVGGVEKIFKRLFRVREGEK 111

Query: 62  LLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKR 121
           LL ++ CYLSTTAGPIAGL+F+ST  +AFCS+R I  SS +GE+ +I YKV+IP+ K+K 
Sbjct: 112 LLKASQCYLSTTAGPIAGLLFISTDKIAFCSERAIKFSSANGELVRIHYKVLIPLRKIKI 171

Query: 122 VNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVS 167
            NQ  N   P++KYI++VT D+F+FWFMGFLN++KAFK++Q  +S
Sbjct: 172 ANQSENTKKPSQKYIEIVTTDNFEFWFMGFLNYQKAFKYLQQALS 216

[Close]

BLAST of CSPI05G20270 vs. TrEMBL
Match: A0A0A0KMT0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G352650 PE=4 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 4.5e-51
Identity = 92/165 (55.76%), Postives = 130/165 (78.79%), Query Frame = 1


Query: 2   IRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDK 61
           + +LG+R  SF +G+ EHV+L   I++TVKGKL LG ++LQ GG   IF ++FN+  G+K
Sbjct: 60  VNKLGKRADSFAHGVREHVRLGSKISETVKGKLSLGARILQVGGLRKIFKQLFNVGDGEK 119

Query: 62  LLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKR 121
           LL + HCYLSTTAGPIAGL+F+ST+ +AFCSDR I +SSP GE+ +  YKV+IPV K++R
Sbjct: 120 LLKAVHCYLSTTAGPIAGLLFISTNKIAFCSDRSIKVSSPSGELIRFHYKVVIPVGKIER 179

Query: 122 VNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVS 167
           +NQ  NV  P++KY+++VTVD+FDFWFMGFLN+ K  +++Q+ +S
Sbjct: 180 INQSENVKKPSQKYMEIVTVDNFDFWFMGFLNYHKTLQYLQDVIS 224

[Close]

BLAST of CSPI05G20270 vs. TAIR10
Match: AT5G08350.1 (AT5G08350.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 183.7 bits (465), Expect = 1.0e-46
Identity = 83/164 (50.61%), Postives = 117/164 (71.34%), Query Frame = 1


Query: 12  FVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDKLLNSAHCYLS 71
           F NG+ +  K+ P + +TVK KL LG ++LQ GG E IF ++F +  G+KL     CYLS
Sbjct: 59  FTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYLS 118

Query: 72  TTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNNP 131
           TTAGPIAGL+F+S+  +AFCS+R I + SP G+I ++ YKV IP+ K+ RVNQ  N   P
Sbjct: 119 TTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQNTKKP 178

Query: 132 AKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSEDWPEE 176
           ++KY++VVTVD FDFWFMGFL+++KAF  ++  +S+  ED  E+
Sbjct: 179 SQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALSLSFEDNKEQ 222

[Close]

BLAST of CSPI05G20270 vs. TAIR10
Match: AT5G23370.1 (AT5G23370.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 179.5 bits (454), Expect = 2.0e-45
Identity = 79/161 (49.07%), Postives = 116/161 (72.05%), Query Frame = 1


Query: 7   RRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDKLLNSA 66
           ++  SF NG+ +  KL P + +TVK KL LG ++LQ GG E I+ ++F +   +KL  + 
Sbjct: 55  KKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAY 114

Query: 67  HCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRN 126
            CYLSTTAGPIAGL+F+S+  +AFCS+R I ++SP GE+ ++ YKV IP+ K+  VNQ  
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQ 174

Query: 127 NVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSV 168
           N   P++KY++VVTVD FDFWFMGFL+++KAF  ++  +S+
Sbjct: 175 NTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALSL 215

[Close]

BLAST of CSPI05G20270 vs. TAIR10
Match: AT5G23350.1 (AT5G23350.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 173.7 bits (439), Expect = 1.1e-43
Identity = 76/160 (47.50%), Postives = 114/160 (71.25%), Query Frame = 1


Query: 11  SFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDKLLNSAHCYL 70
           SF NG  +  KL P + +TVK KL LG K+LQ GG E I+ ++F +   +KL  +  CYL
Sbjct: 121 SFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYL 180

Query: 71  STTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNN 130
           STTAGPIAGL+F+S+  +AFCS+R I ++SP G + ++ YKV IP+ K+  VNQ  N   
Sbjct: 181 STTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQNTKK 240

Query: 131 PAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSE 171
           P++KY+++VT+D+FDFWFMGF++++KAF  ++  ++ D +
Sbjct: 241 PSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALNNDEQ 280

[Close]

BLAST of CSPI05G20270 vs. TAIR10
Match: AT5G23360.1 (AT5G23360.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 168.3 bits (425), Expect = 4.5e-42
Identity = 74/160 (46.25%), Postives = 113/160 (70.62%), Query Frame = 1


Query: 11  SFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDKLLNSAHCYL 70
           SF NG  +  KL P + +TVK KL LG K+LQ GG E I+ ++F +   +KL  +  CYL
Sbjct: 51  SFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQCYL 110

Query: 71  STTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNN 130
           STT G IAGL+F+S+  +AFCS+R I ++SP G++ ++ YKV IP+ K+  VNQ  N   
Sbjct: 111 STTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQNTKK 170

Query: 131 PAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSE 171
           P+++Y++VVTVD++DFWFMGF++++KAF  ++  ++ D +
Sbjct: 171 PSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALNEDEQ 210

[Close]

BLAST of CSPI05G20270 vs. TAIR10
Match: AT2G22475.1 (AT2G22475.1 GRAM domain family protein)

HSP 1 Score: 135.2 bits (339), Expect = 4.2e-32
Identity = 63/165 (38.18%), Postives = 109/165 (66.06%), Query Frame = 1


Query: 2   IRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDK 61
           + E  ++  S      +H++ +P+ A    G++   TK+   GG E IF + F  +  ++
Sbjct: 129 VAEAAKKTESLAGNTWQHLRTAPSFADAAMGRIAQSTKVFAEGGYEKIFRQTFETDPEEQ 188

Query: 62  LLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGK-IFYKVMIPVNKVK 121
           LLNS  CYLST+AGP+ G++++S+  +A+CSD P  +S  +G+  +  +YKV+IP++++K
Sbjct: 189 LLNSFACYLSTSAGPVMGVLYISSAKLAYCSDNP--LSYKNGDQTEWSYYKVVIPLHQLK 248

Query: 122 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGV 166
            VN   ++ NPA+KYIQV++VD+ +FWFMGFLN++ A   +Q+ +
Sbjct: 249 AVNPSASIVNPAEKYIQVISVDNHEFWFMGFLNYDGAVTSLQDSL 291

[Close]

BLAST of CSPI05G20270 vs. NCBI nr
Match: gi|449458799|ref|XP_004147134.1| (PREDICTED: GEM-like protein 4 [Cucumis sativus])

HSP 1 Score: 395.2 bits (1014), Expect = 6.4e-107
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 1


Query: 1   MIRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGD 60
           MIRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGD
Sbjct: 1   MIRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGD 60

Query: 61  KLLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
           KLLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSEDWPEEAQRKC 180
           RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSEDWPEEAQRKC
Sbjct: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSEDWPEEAQRKC 180

Query: 181 CPGKFKTL 189
           CPGKFKTL
Sbjct: 181 CPGKFKTL 188

[Close]

BLAST of CSPI05G20270 vs. NCBI nr
Match: gi|659090209|ref|XP_008445893.1| (PREDICTED: GEM-like protein 4 [Cucumis melo])

HSP 1 Score: 329.7 bits (844), Expect = 3.3e-87
Identity = 156/168 (92.86%), Postives = 163/168 (97.02%), Query Frame = 1


Query: 1   MIRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGD 60
           MIRELGR+GYSFVNGILEHVKLSPNIAKTVKGKLWLGTKL+QFGGSE IFHKMFNLE GD
Sbjct: 1   MIRELGRKGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFHKMFNLEQGD 60

Query: 61  KLLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
           KLLNSAHCYLSTTAGPIAGL+FVSTHVVAFCSDRPIIISSPHGE+GK+FYKVMIPVNKVK
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEVGKVFYKVMIPVNKVK 120

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVD 169
           RVNQRNN NNPAKKYIQVVTVDDFDFWFMGFLN+EK FKFIQ GVS+D
Sbjct: 121 RVNQRNNENNPAKKYIQVVTVDDFDFWFMGFLNYEKTFKFIQKGVSMD 168

[Close]

BLAST of CSPI05G20270 vs. NCBI nr
Match: gi|596296327|ref|XP_007227154.1| (hypothetical protein PRUPE_ppa026708mg [Prunus persica])

HSP 1 Score: 211.1 bits (536), Expect = 1.7e-51
Identity = 93/165 (56.36%), Postives = 130/165 (78.79%), Query Frame = 1


Query: 2   IRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDK 61
           + +LG++  SF +G+ EHV+L P I +TVKGKL LG K++Q GG E +F   F++  G+K
Sbjct: 57  MNKLGKKADSFAHGVKEHVRLGPKITETVKGKLSLGAKIIQVGGLEKVFKHAFSVRAGEK 116

Query: 62  LLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKR 121
           LL  + CYLSTTAGPIAGL+F+ST  +AFCS+R I +SSP+GE+ ++ YKV+IPVNK+KR
Sbjct: 117 LLKVSQCYLSTTAGPIAGLLFISTDKIAFCSERSIKLSSPNGELIRVHYKVVIPVNKIKR 176

Query: 122 VNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVS 167
           VNQ  NV  P++KYI++VTVD+FDFWFMGFLN++K  K+++  +S
Sbjct: 177 VNQSENVKKPSEKYIEIVTVDNFDFWFMGFLNYQKTLKYLEEAIS 221

[Close]

BLAST of CSPI05G20270 vs. NCBI nr
Match: gi|590689422|ref|XP_007043224.1| (GRAM domain-containing protein / ABA-responsive protein-related [Theobroma cacao])

HSP 1 Score: 210.7 bits (535), Expect = 2.2e-51
Identity = 95/169 (56.21%), Postives = 129/169 (76.33%), Query Frame = 1


Query: 5   LGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDKLLN 64
           LG++  +F +G+ EHV+L P I +TVKGKL LG ++LQ GG E IF ++F++  G+KLL 
Sbjct: 63  LGKKADTFAHGVREHVRLGPKITETVKGKLSLGARILQVGGVEKIFKRLFSVREGEKLLK 122

Query: 65  SAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQ 124
           +  CYLSTTAGPIAGL+FVS+  VAFCS+R I I SP GE+ ++ YKV+IP+ K+K VNQ
Sbjct: 123 ACQCYLSTTAGPIAGLLFVSSEKVAFCSERSIKIPSPKGELPRVHYKVLIPLEKIKGVNQ 182

Query: 125 RNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSEDWP 174
             N+  P++KYI++VTVD FDFWFMGFLN+ KAFK++Q  +S   +D P
Sbjct: 183 SENIKKPSQKYIEMVTVDGFDFWFMGFLNYRKAFKYLQQAISQRLDDVP 231

[Close]

BLAST of CSPI05G20270 vs. NCBI nr
Match: gi|225463675|ref|XP_002275935.1| (PREDICTED: GEM-like protein 4 [Vitis vinifera])

HSP 1 Score: 210.3 bits (534), Expect = 2.9e-51
Identity = 93/165 (56.36%), Postives = 131/165 (79.39%), Query Frame = 1


Query: 2   IRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDK 61
           + +LG++  SFVNG+ EHV+L+PNI++TVKGKL LG ++LQ GG E IF ++F +  G+K
Sbjct: 52  MNKLGKKADSFVNGVREHVRLAPNISETVKGKLSLGARILQVGGVEKIFKRLFRVREGEK 111

Query: 62  LLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKR 121
           LL ++ CYLSTTAGPIAGL+F+ST  +AFCS+R I  SS +GE+ +I YKV+IP+ K+K 
Sbjct: 112 LLKASQCYLSTTAGPIAGLLFISTDKIAFCSERAIKFSSANGELVRIHYKVLIPLRKIKI 171

Query: 122 VNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVS 167
            NQ  N   P++KYI++VT D+F+FWFMGFLN++KAFK++Q  +S
Sbjct: 172 ANQSENTKKPSQKYIEIVTTDNFEFWFMGFLNYQKAFKYLQQALS 216

The following BLAST results are available for this feature:

BLAST of CSPI05G20270 vs. Swiss-Prot


Match Name	E-value	Identity	Description
GEML4_ARATH	1.8e-45	50.61	GEM-like protein 4 OS=Arabidopsis thaliana GN=At5g08350 PE=2 SV=1	[more]
GEML8_ARATH	3.5e-44	49.07	Putative GEM-like protein 8 OS=Arabidopsis thaliana GN=At5g23370 PE=3 SV=1	[more]
GEML6_ARATH	1.9e-42	47.50	GEM-like protein 6 OS=Arabidopsis thaliana GN=At5g23350 PE=2 SV=2	[more]
GEML7_ARATH	8.0e-41	46.25	GEM-like protein 7 OS=Arabidopsis thaliana GN=At5g23360 PE=2 SV=1	[more]
GEM_ARATH	7.5e-31	38.18	GLABRA2 expression modulator OS=Arabidopsis thaliana GN=GEM PE=1 SV=1	[more]

BLAST of CSPI05G20270 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPM0_CUCSA	4.5e-107	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G577990 PE=4 SV=1	[more]
M5Y243_PRUPE	1.2e-51	56.36	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa026708mg PE=4 SV=1	[more]
A0A061E267_THECC	1.6e-51	56.21	GRAM domain-containing protein / ABA-responsive protein-related OS=Theobroma cac...	[more]
D7TY17_VITVI	2.0e-51	56.36	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_10s0071g00320 PE=4 SV=...	[more]
A0A0A0KMT0_CUCSA	4.5e-51	55.76	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G352650 PE=4 SV=1	[more]

BLAST of CSPI05G20270 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G08350.1	1.0e-46	50.61	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23370.1	2.0e-45	49.07	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23350.1	1.1e-43	47.50	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23360.1	4.5e-42	46.25	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT2G22475.1	4.2e-32	38.18	GRAM domain family protein	[more]

BLAST of CSPI05G20270 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449458799\|ref\|XP_004147134.1\|	6.4e-107	100.00	PREDICTED: GEM-like protein 4 [Cucumis sativus]	[more]
gi\|659090209\|ref\|XP_008445893.1\|	3.3e-87	92.86	PREDICTED: GEM-like protein 4 [Cucumis melo]	[more]
gi\|596296327\|ref\|XP_007227154.1\|	1.7e-51	56.36	hypothetical protein PRUPE_ppa026708mg [Prunus persica]	[more]
gi\|590689422\|ref\|XP_007043224.1\|	2.2e-51	56.21	GRAM domain-containing protein / ABA-responsive protein-related [Theobroma cacao...	[more]
gi\|225463675\|ref\|XP_002275935.1\|	2.9e-51	56.36	PREDICTED: GEM-like protein 4 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR004182	GRAM

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G20270.1	CSPI05G20270.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004182	GRAM domain	PFAM	PF02893	GRAM	coord: 49..124 score: 3.6
IPR004182	GRAM domain	SMART	SM00568	gram2001c	coord: 48..126 score: 7.3
None	No IPR available	PANTHER	PTHR31969	FAMILY NOT NAMED	coord: 1..172 score: 1.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI05G20270	Cla020045	Watermelon (97103) v1	cpiwmB439
CSPI05G20270	Csa5G577990	Cucumber (Chinese Long) v2	cpicuB239
CSPI05G20270	ClCG02G014170	Watermelon (Charleston Gray)	cpiwcgB397
CSPI05G20270	Lsi04G007830	Bottle gourd (USVL1VR-Ls)	cpilsiB372
CSPI05G20270	MELO3C011758.2	Melon (DHL92) v3.6.1	cpimedB363
CSPI05G20270	CsaV3_5G029030	Cucumber (Chinese Long) v3	cpicucB284
CSPI05G20270	Cla97C02G039930	Watermelon (97103) v2	cpiwmbB384
CSPI05G20270	Bhi07G000909	Wax gourd	cpiwgoB521
CSPI05G20270	Cucsa.280920	Cucumber (Gy14) v1	cgycpiB421
CSPI05G20270	CmaCh18G008130	Cucurbita maxima (Rimu)	cmacpiB420
CSPI05G20270	CmaCh04G012650	Cucurbita maxima (Rimu)	cmacpiB733
CSPI05G20270	CmoCh04G013360	Cucurbita moschata (Rifu)	cmocpiB725
CSPI05G20270	CmoCh18G008150	Cucurbita moschata (Rifu)	cmocpiB408
CSPI05G20270	Cp4.1LG09g04850	Cucurbita pepo (Zucchini)	cpecpiB043
CSPI05G20270	Cp4.1LG01g10160	Cucurbita pepo (Zucchini)	cpecpiB417
CSPI05G20270	CsGy5G019800	Cucumber (Gy14) v2	cgybcpiB225

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI05G20270	Cucumber (Chinese Long) v3	cpicucB295
CSPI05G20270	Watermelon (97103) v2	cpiwmbB406
CSPI05G20270	Wax gourd	cpiwgoB507
CSPI05G20270	Wax gourd	cpiwgoB525
CSPI05G20270	Wild cucumber (PI 183967)	cpicpiB176
CSPI05G20270	Cucumber (Gy14) v1	cgycpiB434
CSPI05G20270	Cucurbita moschata (Rifu)	cmocpiB417
CSPI05G20270	Melon (DHL92) v3.5.1	cpimeB369
CSPI05G20270	Cucurbita pepo (Zucchini)	cpecpiB041
CSPI05G20270	Bottle gourd (USVL1VR-Ls)	cpilsiB377
CSPI05G20270	Melon (DHL92) v3.6.1	cpimedB371
CSPI05G20270	Silver-seed gourd	carcpiB0760
CSPI05G20270	Silver-seed gourd	carcpiB0879
CSPI05G20270	Silver-seed gourd	carcpiB0881