CmoCh12G004850 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh12G004850
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Calcium-binding EF-hand family protein, putative
Location	Cmo_Chr12 : 2983094 .. 2983354 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTATGGATCAAGCAAGCTAAAGCTCTCCAAATGCGAGATCAAAGAGATCTTTAGAGAGCACGATATCGATGGAGATGGTCAATTGAGCATCAGCGAGCTAGTAAAGGCCTTCGGGTTCCTAGGCAATATCCTTCCCTTCTATAAAGCTCACTATGGATTGGCTCATGCCGATGCGGATGGCGATGGCTTCATCGATGAGGATGAACTCGACAAGCTCGTTGAATATGCCGAGAAGTTTCAGCATAAGAGGCACTGA

mRNA sequence

ATGTCTTATGGATCAAGCAAGCTAAAGCTCTCCAAATGCGAGATCAAAGAGATCTTTAGAGAGCACGATATCGATGGAGATGGTCAATTGAGCATCAGCGAGCTAGTAAAGGCCTTCGGGTTCCTAGGCAATATCCTTCCCTTCTATAAAGCTCACTATGGATTGGCTCATGCCGATGCGGATGGCGATGGCTTCATCGATGAGGATGAACTCGACAAGCTCGTTGAATATGCCGAGAAGTTTCAGCATAAGAGGCACTGA

Coding sequence (CDS)

ATGTCTTATGGATCAAGCAAGCTAAAGCTCTCCAAATGCGAGATCAAAGAGATCTTTAGAGAGCACGATATCGATGGAGATGGTCAATTGAGCATCAGCGAGCTAGTAAAGGCCTTCGGGTTCCTAGGCAATATCCTTCCCTTCTATAAAGCTCACTATGGATTGGCTCATGCCGATGCGGATGGCGATGGCTTCATCGATGAGGATGAACTCGACAAGCTCGTTGAATATGCCGAGAAGTTTCAGCATAAGAGGCACTGA

Homology

[Close]

BLAST of CmoCh12G004850 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.8e-06
Identity = 30/73 (41.10%), Postives = 41/73 (56.16%), Query Frame = 1


Query: 2   SYGSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADAD 61
           S G S     + E++ +FR+ D +GDG++S SEL   F  LG+     +    +A ADAD
Sbjct: 28  SGGGSPAPTPEEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADAD 87

Query: 62  GDGFIDEDELDKL 75
           GDGFI  DE   L
Sbjct: 88  GDGFISLDEFAAL 100

[Close]

BLAST of CmoCh12G004850 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 7.6e-30
Identity = 62/85 (72.94%), Postives = 79/85 (92.94%), Query Frame = 1


Query: 1  MSYGSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADA 60
          MS  +++L+LS+ +IKEIFREHDIDGDG+LS++E+VKAFG  G+ILPFYKAHYG+AHAD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGFIDEDELDKLVEYAEKFQHKR 86
          +GDGFI+E+ELDKLV+YA+KFQHKR
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKR 85

[Close]

BLAST of CmoCh12G004850 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 3.0e-26
Identity = 57/82 (69.51%), Postives = 69/82 (84.15%), Query Frame = 1


Query: 4  GSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADADGD 63
          G SK   S+ E+KEIFREHDIDGDG LSISEL+KA GFLG+ +PFYKAHYG+A+ D +GD
Sbjct: 5  GVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGD 64

Query: 64 GFIDEDELDKLVEYAEKFQHKR 86
          G I EDELDKL++YAE+FQ +R
Sbjct: 65 GLISEDELDKLIDYAERFQKRR 86

[Close]

BLAST of CmoCh12G004850 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.1e-17
Identity = 45/81 (55.56%), Postives = 63/81 (77.78%), Query Frame = 1


Query: 5   SSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADADGDG 64
           +S ++ S+ +IKEIF+ HD D DG L++ E+ KAF FLG ILP YKA++G+ +ADA+ DG
Sbjct: 96  ASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDG 155

Query: 65  FIDEDELDKLVEYAEKFQHKR 86
            I +DEL+KLV+YA KF +KR
Sbjct: 156 LISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of CmoCh12G004850 vs. TrEMBL
Match: A0A0A0LNG6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359930 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 4.4e-17
Identity = 49/83 (59.04%), Postives = 62/83 (74.70%), Query Frame = 1


Query: 2   SYGSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADAD 61
           S G  K++LSK E++EIF EHDIDGDG L+ SELVKAF   G+   F KA+Y L  ADA+
Sbjct: 76  SCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLADAN 135

Query: 62  GDGFIDEDELDKLVEYAEKFQHK 85
           GDGFI  DELDK++EYA++  +K
Sbjct: 136 GDGFICLDELDKVLEYADRVINK 158

[Close]

BLAST of CmoCh12G004850 vs. TrEMBL
Match: A0A0A0LKW4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.7e-16
Identity = 46/78 (58.97%), Postives = 57/78 (73.08%), Query Frame = 1


Query: 6  SKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADADGDGF 65
          S  KL K E+KE+FREHD +GDG+LSI EL +AFG  G   P Y+A +GL  AD DGDGF
Sbjct: 11 SGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGF 70

Query: 66 IDEDELDKLVEYAEKFQH 84
          I E ELDK+V+YA K ++
Sbjct: 71 ISEQELDKVVDYAIKCKY 88

[Close]

BLAST of CmoCh12G004850 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 137.5 bits (345), Expect = 1.1e-29
Identity = 62/85 (72.94%), Postives = 79/85 (92.94%), Query Frame = 1


Query: 1  MSYGSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADA 60
          MS  +++L+LS+ +IKEIFREHDIDGDG+LS++E+VKAFG  G+ILPFYKAHYG+AHAD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGFIDEDELDKLVEYAEKFQHKR 86
          +GDGFI+E+ELDKLV+YA+KFQHKR
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKR 85

[Close]

BLAST of CmoCh12G004850 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 135.6 bits (340), Expect = 4.2e-29
Identity = 61/81 (75.31%), Postives = 77/81 (95.06%), Query Frame = 1


Query: 5   SSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADADGDG 64
           S++L+LS+ +IKEIFREHDIDGDG+LS++E+VKAFG  G+ILPFYKAHYG+AHAD +GDG
Sbjct: 86  STRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDG 145

Query: 65  FIDEDELDKLVEYAEKFQHKR 86
           FI+E+ELDKLV+YA+KFQHKR
Sbjct: 146 FIEEEELDKLVDYAQKFQHKR 166

[Close]

BLAST of CmoCh12G004850 vs. NCBI nr
Match: gi|700207406|gb|KGN62525.1| (hypothetical protein Csa_2G359940 [Cucumis sativus])

HSP 1 Score: 125.6 bits (314), Expect = 4.3e-26
Identity = 57/82 (69.51%), Postives = 69/82 (84.15%), Query Frame = 1


Query: 4  GSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADADGD 63
          G SK   S+ E+KEIFREHDIDGDG LSISEL+KA GFLG+ +PFYKAHYG+A+ D +GD
Sbjct: 5  GVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGD 64

Query: 64 GFIDEDELDKLVEYAEKFQHKR 86
          G I EDELDKL++YAE+FQ +R
Sbjct: 65 GLISEDELDKLIDYAERFQKRR 86

[Close]

BLAST of CmoCh12G004850 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 97.1 bits (240), Expect = 1.6e-17
Identity = 45/81 (55.56%), Postives = 63/81 (77.78%), Query Frame = 1


Query: 5   SSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADADGDG 64
           +S ++ S+ +IKEIF+ HD D DG L++ E+ KAF FLG ILP YKA++G+ +ADA+ DG
Sbjct: 96  ASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDG 155

Query: 65  FIDEDELDKLVEYAEKFQHKR 86
            I +DEL+KLV+YA KF +KR
Sbjct: 156 LISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of CmoCh12G004850 vs. NCBI nr
Match: gi|449470192|ref|XP_004152802.1| (PREDICTED: calcium-binding protein-like [Cucumis sativus])

HSP 1 Score: 95.1 bits (235), Expect = 6.2e-17
Identity = 49/83 (59.04%), Postives = 62/83 (74.70%), Query Frame = 1


Query: 2   SYGSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADAD 61
           S G  K++LSK E++EIF EHDIDGDG L+ SELVKAF   G+   F KA+Y L  ADA+
Sbjct: 76  SCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLADAN 135

Query: 62  GDGFIDEDELDKLVEYAEKFQHK 85
           GDGFI  DELDK++EYA++  +K
Sbjct: 136 GDGFICLDELDKVLEYADRVINK 158

The following BLAST results are available for this feature:

BLAST of CmoCh12G004850 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML10_ORYSJ	3.8e-06	41.10	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ...	[more]

BLAST of CmoCh12G004850 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRC1_CUCSA	7.6e-30	72.94	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0LKX1_CUCSA	3.0e-26	69.51	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]
A0A0A0KIY4_CUCSA	1.1e-17	55.56	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1	[more]
A0A0A0LNG6_CUCSA	4.4e-17	59.04	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359930 PE=4 SV=1	[more]
A0A0A0LKW4_CUCSA	1.7e-16	58.97	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1	[more]

BLAST of CmoCh12G004850 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CmoCh12G004850 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207397\|gb\|KGN62516.1\|	1.1e-29	72.94	hypothetical protein Csa_2G358870 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	4.2e-29	75.31	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|700207406\|gb\|KGN62525.1\|	4.3e-26	69.51	hypothetical protein Csa_2G359940 [Cucumis sativus]	[more]
gi\|700194513\|gb\|KGN49690.1\|	1.6e-17	55.56	hypothetical protein Csa_5G068170 [Cucumis sativus]	[more]
gi\|449470192\|ref\|XP_004152802.1\|	6.2e-17	59.04	PREDICTED: calcium-binding protein-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh12G004850.1	CmoCh12G004850.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 14..39 score: 1.
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 58..75 score: 3.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 14..42 score: 7.3E-4coord: 50..78 score:
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 56..81 score: 8.767coord: 14..45 score: 12
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 13..79 score: 5.4
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 8..74 score: 2.06
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 23..35 score: -coord: 59..71 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 5..78 score: 3.2
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 5..78 score: 3.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh12G004850	CmaCh12G005430	Cucurbita maxima (Rimu)	cmacmoB176
CmoCh12G004850	MELO3C010869.2	Melon (DHL92) v3.6.1	cmomedB161
CmoCh12G004850	CsGy2G019080	Cucumber (Gy14) v2	cgybcmoB171

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh12G004850	Cucurbita pepo (Zucchini)	cmocpeB154
CmoCh12G004850	Cucurbita pepo (Zucchini)	cmocpeB166
CmoCh12G004850	Cucurbita pepo (Zucchini)	cmocpeB172
CmoCh12G004850	Cucurbita pepo (Zucchini)	cmocpeB174
CmoCh12G004850	Bottle gourd (USVL1VR-Ls)	cmolsiB167
CmoCh12G004850	Bottle gourd (USVL1VR-Ls)	cmolsiB175
CmoCh12G004850	Cucumber (Gy14) v2	cgybcmoB449
CmoCh12G004850	Melon (DHL92) v3.6.1	cmomedB188
CmoCh12G004850	Silver-seed gourd	carcmoB0108
CmoCh12G004850	Silver-seed gourd	carcmoB0257
CmoCh12G004850	Silver-seed gourd	carcmoB1042
CmoCh12G004850	Cucumber (Chinese Long) v3	cmocucB0186
CmoCh12G004850	Cucumber (Chinese Long) v3	cmocucB0200
CmoCh12G004850	Watermelon (97103) v2	cmowmbB178
CmoCh12G004850	Watermelon (97103) v2	cmowmbB166
CmoCh12G004850	Wax gourd	cmowgoB0190
CmoCh12G004850	Wax gourd	cmowgoB0222
CmoCh12G004850	Cucurbita moschata (Rifu)	cmocmoB004
CmoCh12G004850	Cucurbita moschata (Rifu)	cmocmoB141
CmoCh12G004850	Cucurbita moschata (Rifu)	cmocmoB156
CmoCh12G004850	Cucumber (Gy14) v1	cgycmoB0641
CmoCh12G004850	Cucurbita maxima (Rimu)	cmacmoB032
CmoCh12G004850	Cucurbita maxima (Rimu)	cmacmoB453
CmoCh12G004850	Cucurbita maxima (Rimu)	cmacmoB770
CmoCh12G004850	Wild cucumber (PI 183967)	cmocpiB160
CmoCh12G004850	Wild cucumber (PI 183967)	cmocpiB172
CmoCh12G004850	Cucumber (Chinese Long) v2	cmocuB158
CmoCh12G004850	Cucumber (Chinese Long) v2	cmocuB169
CmoCh12G004850	Melon (DHL92) v3.5.1	cmomeB146
CmoCh12G004850	Melon (DHL92) v3.5.1	cmomeB171
CmoCh12G004850	Watermelon (Charleston Gray)	cmowcgB149
CmoCh12G004850	Watermelon (Charleston Gray)	cmowcgB161
CmoCh12G004850	Watermelon (97103) v1	cmowmB134
CmoCh12G004850	Watermelon (97103) v1	cmowmB145