CmoCh12G004850.1 (mRNA) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh12G004850.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Calcium-binding EF-hand family protein, putative
Location	Cmo_Chr12 : 2983094 .. 2983354 (-)
Sequence length	261

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTATGGATCAAGCAAGCTAAAGCTCTCCAAATGCGAGATCAAAGAGATCTTTAGAGAGCACGATATCGATGGAGATGGTCAATTGAGCATCAGCGAGCTAGTAAAGGCCTTCGGGTTCCTAGGCAATATCCTTCCCTTCTATAAAGCTCACTATGGATTGGCTCATGCCGATGCGGATGGCGATGGCTTCATCGATGAGGATGAACTCGACAAGCTCGTTGAATATGCCGAGAAGTTTCAGCATAAGAGGCACTGA

mRNA sequence

ATGTCTTATGGATCAAGCAAGCTAAAGCTCTCCAAATGCGAGATCAAAGAGATCTTTAGAGAGCACGATATCGATGGAGATGGTCAATTGAGCATCAGCGAGCTAGTAAAGGCCTTCGGGTTCCTAGGCAATATCCTTCCCTTCTATAAAGCTCACTATGGATTGGCTCATGCCGATGCGGATGGCGATGGCTTCATCGATGAGGATGAACTCGACAAGCTCGTTGAATATGCCGAGAAGTTTCAGCATAAGAGGCACTGA

Coding sequence (CDS)

ATGTCTTATGGATCAAGCAAGCTAAAGCTCTCCAAATGCGAGATCAAAGAGATCTTTAGAGAGCACGATATCGATGGAGATGGTCAATTGAGCATCAGCGAGCTAGTAAAGGCCTTCGGGTTCCTAGGCAATATCCTTCCCTTCTATAAAGCTCACTATGGATTGGCTCATGCCGATGCGGATGGCGATGGCTTCATCGATGAGGATGAACTCGACAAGCTCGTTGAATATGCCGAGAAGTTTCAGCATAAGAGGCACTGA

Homology

[Close]

BLAST of CmoCh12G004850.1 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.8e-06
Identity = 30/73 (41.10%), Postives = 41/73 (56.16%), Query Frame = 1


Query: 2   SYGSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADAD 61
           S G S     + E++ +FR+ D +GDG++S SEL   F  LG+     +    +A ADAD
Sbjct: 28  SGGGSPAPTPEEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADAD 87

Query: 62  GDGFIDEDELDKL 75
           GDGFI  DE   L
Sbjct: 88  GDGFISLDEFAAL 100

[Close]

BLAST of CmoCh12G004850.1 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 7.6e-30
Identity = 62/85 (72.94%), Postives = 79/85 (92.94%), Query Frame = 1


Query: 1  MSYGSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADA 60
          MS  +++L+LS+ +IKEIFREHDIDGDG+LS++E+VKAFG  G+ILPFYKAHYG+AHAD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGFIDEDELDKLVEYAEKFQHKR 86
          +GDGFI+E+ELDKLV+YA+KFQHKR
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKR 85

[Close]

BLAST of CmoCh12G004850.1 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 3.0e-26
Identity = 57/82 (69.51%), Postives = 69/82 (84.15%), Query Frame = 1


Query: 4  GSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADADGD 63
          G SK   S+ E+KEIFREHDIDGDG LSISEL+KA GFLG+ +PFYKAHYG+A+ D +GD
Sbjct: 5  GVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGD 64

Query: 64 GFIDEDELDKLVEYAEKFQHKR 86
          G I EDELDKL++YAE+FQ +R
Sbjct: 65 GLISEDELDKLIDYAERFQKRR 86

[Close]

BLAST of CmoCh12G004850.1 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.1e-17
Identity = 45/81 (55.56%), Postives = 63/81 (77.78%), Query Frame = 1


Query: 5   SSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADADGDG 64
           +S ++ S+ +IKEIF+ HD D DG L++ E+ KAF FLG ILP YKA++G+ +ADA+ DG
Sbjct: 96  ASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDG 155

Query: 65  FIDEDELDKLVEYAEKFQHKR 86
            I +DEL+KLV+YA KF +KR
Sbjct: 156 LISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of CmoCh12G004850.1 vs. TrEMBL
Match: A0A0A0LNG6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359930 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 4.4e-17
Identity = 49/83 (59.04%), Postives = 62/83 (74.70%), Query Frame = 1


Query: 2   SYGSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADAD 61
           S G  K++LSK E++EIF EHDIDGDG L+ SELVKAF   G+   F KA+Y L  ADA+
Sbjct: 76  SCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLADAN 135

Query: 62  GDGFIDEDELDKLVEYAEKFQHK 85
           GDGFI  DELDK++EYA++  +K
Sbjct: 136 GDGFICLDELDKVLEYADRVINK 158

[Close]

BLAST of CmoCh12G004850.1 vs. TrEMBL
Match: A0A0A0LKW4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.7e-16
Identity = 46/78 (58.97%), Postives = 57/78 (73.08%), Query Frame = 1


Query: 6  SKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADADGDGF 65
          S  KL K E+KE+FREHD +GDG+LSI EL +AFG  G   P Y+A +GL  AD DGDGF
Sbjct: 11 SGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGF 70

Query: 66 IDEDELDKLVEYAEKFQH 84
          I E ELDK+V+YA K ++
Sbjct: 71 ISEQELDKVVDYAIKCKY 88

[Close]

BLAST of CmoCh12G004850.1 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 137.5 bits (345), Expect = 1.1e-29
Identity = 62/85 (72.94%), Postives = 79/85 (92.94%), Query Frame = 1


Query: 1  MSYGSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADA 60
          MS  +++L+LS+ +IKEIFREHDIDGDG+LS++E+VKAFG  G+ILPFYKAHYG+AHAD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGFIDEDELDKLVEYAEKFQHKR 86
          +GDGFI+E+ELDKLV+YA+KFQHKR
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKR 85

[Close]

BLAST of CmoCh12G004850.1 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 135.6 bits (340), Expect = 4.2e-29
Identity = 61/81 (75.31%), Postives = 77/81 (95.06%), Query Frame = 1


Query: 5   SSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADADGDG 64
           S++L+LS+ +IKEIFREHDIDGDG+LS++E+VKAFG  G+ILPFYKAHYG+AHAD +GDG
Sbjct: 86  STRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDG 145

Query: 65  FIDEDELDKLVEYAEKFQHKR 86
           FI+E+ELDKLV+YA+KFQHKR
Sbjct: 146 FIEEEELDKLVDYAQKFQHKR 166

[Close]

BLAST of CmoCh12G004850.1 vs. NCBI nr
Match: gi|700207406|gb|KGN62525.1| (hypothetical protein Csa_2G359940 [Cucumis sativus])

HSP 1 Score: 125.6 bits (314), Expect = 4.3e-26
Identity = 57/82 (69.51%), Postives = 69/82 (84.15%), Query Frame = 1


Query: 4  GSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADADGD 63
          G SK   S+ E+KEIFREHDIDGDG LSISEL+KA GFLG+ +PFYKAHYG+A+ D +GD
Sbjct: 5  GVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGD 64

Query: 64 GFIDEDELDKLVEYAEKFQHKR 86
          G I EDELDKL++YAE+FQ +R
Sbjct: 65 GLISEDELDKLIDYAERFQKRR 86

[Close]

BLAST of CmoCh12G004850.1 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 97.1 bits (240), Expect = 1.6e-17
Identity = 45/81 (55.56%), Postives = 63/81 (77.78%), Query Frame = 1


Query: 5   SSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADADGDG 64
           +S ++ S+ +IKEIF+ HD D DG L++ E+ KAF FLG ILP YKA++G+ +ADA+ DG
Sbjct: 96  ASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDG 155

Query: 65  FIDEDELDKLVEYAEKFQHKR 86
            I +DEL+KLV+YA KF +KR
Sbjct: 156 LISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of CmoCh12G004850.1 vs. NCBI nr
Match: gi|449470192|ref|XP_004152802.1| (PREDICTED: calcium-binding protein-like [Cucumis sativus])

HSP 1 Score: 95.1 bits (235), Expect = 6.2e-17
Identity = 49/83 (59.04%), Postives = 62/83 (74.70%), Query Frame = 1


Query: 2   SYGSSKLKLSKCEIKEIFREHDIDGDGQLSISELVKAFGFLGNILPFYKAHYGLAHADAD 61
           S G  K++LSK E++EIF EHDIDGDG L+ SELVKAF   G+   F KA+Y L  ADA+
Sbjct: 76  SCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLADAN 135

Query: 62  GDGFIDEDELDKLVEYAEKFQHK 85
           GDGFI  DELDK++EYA++  +K
Sbjct: 136 GDGFICLDELDKVLEYADRVINK 158

The following BLAST results are available for this feature:

BLAST of CmoCh12G004850.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML10_ORYSJ	3.8e-06	41.10	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ...	[more]

BLAST of CmoCh12G004850.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRC1_CUCSA	7.6e-30	72.94	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0LKX1_CUCSA	3.0e-26	69.51	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]
A0A0A0KIY4_CUCSA	1.1e-17	55.56	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1	[more]
A0A0A0LNG6_CUCSA	4.4e-17	59.04	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359930 PE=4 SV=1	[more]
A0A0A0LKW4_CUCSA	1.7e-16	58.97	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1	[more]

BLAST of CmoCh12G004850.1 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CmoCh12G004850.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207397\|gb\|KGN62516.1\|	1.1e-29	72.94	hypothetical protein Csa_2G358870 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	4.2e-29	75.31	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|700207406\|gb\|KGN62525.1\|	4.3e-26	69.51	hypothetical protein Csa_2G359940 [Cucumis sativus]	[more]
gi\|700194513\|gb\|KGN49690.1\|	1.6e-17	55.56	hypothetical protein Csa_5G068170 [Cucumis sativus]	[more]
gi\|449470192\|ref\|XP_004152802.1\|	6.2e-17	59.04	PREDICTED: calcium-binding protein-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh12G004850	CmoCh12G004850	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh12G004850.1	CmoCh12G004850.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh12G004850.1.CDS.1	CmoCh12G004850.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh12G004850.1.exon.1	CmoCh12G004850.1.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 14..39 score: 1.
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 58..75 score: 3.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 14..42 score: 7.3E-4coord: 50..78 score:
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 56..81 score: 8.767coord: 14..45 score: 12
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 13..79 score: 5.4
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 8..74 score: 2.06
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 23..35 score: -coord: 59..71 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 5..78 score: 3.2
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 5..78 score: 3.2