CmaCh11G013560 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh11G013560 vs. Swiss-Prot
Match: 7OMT_PAPSO ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum GN=7OMT PE=1 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.6e-71
Identity = 148/349 (42.41%), Postives = 209/349 (59.89%), Query Frame = 1

Query: 15  EGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLHCSSP------ 74
           +GQA  ++W+++F F +  A+KCA+EL I DII  HG P+T+ ++  +L  ++P      
Sbjct: 9   KGQA--EIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSPNI 68

Query: 75  -LLHRILRFLVSRGIF-KEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEASPAMLA 134
             L RI+R LV + +F  E     + + Y  T  S+ L   +  +L+PL+L E +P +L 
Sbjct: 69  DYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPILLK 128

Query: 135 AWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAAILDGCQG 194
            W  L    +     PFE AHG ++W  A A+PK N  +N AM CST  ++  +L   + 
Sbjct: 129 PWQYLGKCAQEKSS-PFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLEYKD 188

Query: 195 IFDGV-GSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMFDF 254
            F G+ GSLVDVGGG G+ ++ +VKA   I+GINFDLPHV++T+  +  V+HV GDMF  
Sbjct: 189 GFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDMFVD 248

Query: 255 VPNADAAFIKWTLHDWNDEECITILKNCKEAI-PKNTGKVIIVDAVIDEKEETEQSDVGL 314
           +P ADA  +KW LHDW+DE+C  ILKNC  AI  K  GKVIIVD V+          +GL
Sbjct: 249 IPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFDKMGL 308

Query: 315 MLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 354
           + D++MMAHT  GKERT  EW  ++N  GF R+ V    A   +IE  P
Sbjct: 309 IFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

BLAST of CmaCh11G013560 vs. Swiss-Prot
Match: 6OMT_PAPSO ((RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum GN=6OMT PE=1 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 4.0e-70
Identity = 152/352 (43.18%), Postives = 216/352 (61.36%), Query Frame = 1

Query: 7   ETLSKKEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLHCS 66
           ET+SK +++ QA  ++WK I+GF+E   +KCA++L+IA+ + ++  PM+L +L+S L  +
Sbjct: 2   ETVSKIDQQNQA--KIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVA 61

Query: 67  SPL----LHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEASP 126
            P+    L RI+R+LV   +FK   I      Y+  P ++ L      S+   +L     
Sbjct: 62  QPVNEDRLFRIMRYLVHMELFK---IDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDK 121

Query: 127 AMLAAWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAAILD 186
             LA WH+L   + +G    FE A G  +W Y + NP+ N+L N AM C T LV +A+ +
Sbjct: 122 DFLAPWHHLGDGL-TGNCDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALAN 181

Query: 187 GCQGIF-DGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGD 246
            C+ IF DG+ +LVDVGGG G  +  + KA   I+   +DLPHVI+ SP    +  ++GD
Sbjct: 182 ECKSIFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGD 241

Query: 247 MFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQSD 306
           MF  +P+ADA F+K  LHDWND+ECI ILK CKEA+PK  GKVIIVD VID       + 
Sbjct: 242 MFKSIPSADAIFMKCILHDWNDDECIQILKRCKEALPKG-GKVIIVDVVIDMDSTHPYAK 301

Query: 307 VGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 354
           + L LDL MM +T  GKERT++EW  + +A GF+   VT I A+QSVIE  P
Sbjct: 302 IRLTLDLDMMLNTG-GKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345

BLAST of CmaCh11G013560 vs. Swiss-Prot
Match: 4OMT_COPJA (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 3.8e-68
Identity = 138/340 (40.59%), Postives = 200/340 (58.82%), Query Frame = 1

Query: 17  QAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLHCSS---PLLHRI 76
           +A   +WK I+GF++   ++CA+EL I DII+++  PM L  L+S L  S      L+RI
Sbjct: 13  KAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDNLYRI 72

Query: 77  LRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEASPAMLAAWHNLSA 136
           LR+LV   I +     D    YA  P++ LL+     S+ P++L       +  WH++  
Sbjct: 73  LRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWHSMKD 132

Query: 137 YVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAAILDGCQGIFDGVGS 196
            +   G   FE A GM +W Y   +P  ++L NE M   T L+ ++++ G + +F G+ S
Sbjct: 133 GLSDNGT-AFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQGIDS 192

Query: 197 LVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMFDFVPNADAAF 256
           LVDVGGGNG T+  +  A   I+   FDLPHVI+ S     +E + GDMF  VP+A A  
Sbjct: 193 LVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFKSVPSAQAII 252

Query: 257 IKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQSDVGLMLDLVMMAH 316
           +K  LHDWNDE+ I ILK C+ A+PK+ GKVIIVD  +DE+ + E S   L+LD+ M+ +
Sbjct: 253 LKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDIDMLVN 312

Query: 317 TNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 354
           T  GKERT++ W  ++ + GFS   +  I AIQSVIE  P
Sbjct: 313 TG-GKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350

BLAST of CmaCh11G013560 vs. Swiss-Prot
Match: 4OMT2_PAPSO (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum GN=4'OMT2 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.4e-68
Identity = 132/337 (39.17%), Postives = 202/337 (59.94%), Query Frame = 1

Query: 21  QMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTL---HCSSPLLHRILRFL 80
           Q+W  I+GF++   ++CA+E+ IADII+++   +TL QL++ L   + SS  L+R++R+L
Sbjct: 22  QLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVRYL 81

Query: 81  VSRGIFKEYSISDDLIG-YAQTPLSRLLATKNGSSLAPLMLLEASPAMLAAWHNLSAYVK 140
           V   I ++ + +  +   Y+  P+  LL      S+ P++L       + +WH +   + 
Sbjct: 82  VHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEGLG 141

Query: 141 SGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAAILDGCQGIFDGVGSLVD 200
           +G    FE   GMD+W+Y   NP  ++L NE M   T L+   +++ C+  F G+ SLVD
Sbjct: 142 NGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQGLDSLVD 201

Query: 201 VGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMFDFVPNADAAFIKW 260
           +GGGNG T+  + +A   I+   +DLPHV++ S     +E V GDMF  VP+A A  +K 
Sbjct: 202 IGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQAILLKL 261

Query: 261 TLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQSDVGLMLDLVMMAHTNT 320
            LHDW DEEC+ ILK CKEAIPK TGKVIIVD  ++E+   E +   L+LD+ M+ +T  
Sbjct: 262 ILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLVNTG- 321

Query: 321 GKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 354
           G+ERT  +W  ++   GF    +  I+AIQSVIE  P
Sbjct: 322 GRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357

BLAST of CmaCh11G013560 vs. Swiss-Prot
Match: 6OMT_COPJA ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.1e-67
Identity = 142/346 (41.04%), Postives = 208/346 (60.12%), Query Frame = 1

Query: 12  KEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLHCSSPL-- 71
           K++   +  ++W +I+GF+E   +KCA++L +A+II + G+ MTL +LSS L  S P+  
Sbjct: 4   KKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLP-SQPVNE 63

Query: 72  --LHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEASPAMLAA 131
             L+R++R+LV   +F + SI  +L  Y   P ++ L       +   +L       +A 
Sbjct: 64  DALYRVMRYLVHMKLFTKASIDGEL-RYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAP 123

Query: 132 WHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAAILDGCQGI 191
           WH L   +       FE A G ++W Y A +P+ N+L NEAM   + L+++A++  C  I
Sbjct: 124 WHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNI 183

Query: 192 FDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMFDFVP 251
           F+G+ +LVDVGGG G  +  +  A   I+   +DLPHVI+ SP Y  V  VAGDMF F+P
Sbjct: 184 FNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIP 243

Query: 252 NADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQSDVGLMLD 311
            ADA  +K  LHDW+D+ECI ILK CKEA+P   GKVIIVD V++ + E   + + L LD
Sbjct: 244 KADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLD 303

Query: 312 LVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 354
           L MM +T  GKERT +EW  +I+  G+    +T I A+QSVIE  P
Sbjct: 304 LDMMLNTG-GKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346

BLAST of CmaCh11G013560 vs. TrEMBL
Match: A0A0A0K629_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G018730 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 1.6e-134
Identity = 233/357 (65.27%), Postives = 291/357 (81.51%), Query Frame = 1

Query: 1   MERTDKETLSKKEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLS 60
           ME   KE+ S+KEE+GQAIIQMW+YIF F+EMAAIKC ++L+IADIIE +GSP+TL QLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLHCSSPLLHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEA 120
           STL+CSS LLHRILRFL+ RGIFK+ +I ++ IGY+QTP+SRLLAT   +S+APL+LLE 
Sbjct: 61  STLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLET 120

Query: 121 SPAMLAAWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAAI 180
           SP MLA W +LS ++K+    PFE AHG D+WRYA AN +HN L NEAM C   ++V+AI
Sbjct: 121 SPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAI 180

Query: 181 LDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAG 240
           ++GC  +FDGV  LVDVGGGNG+TLS++VKA  W++GINFDLPHV+  SP YE VEHVAG
Sbjct: 181 IEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAG 240

Query: 241 DMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVID----EKEE 300
           +MFDFVPNAD AF+KW LHDW+DEECI ILK CKE+IPK+ GKVII++A+I+    EK+ 
Sbjct: 241 NMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKN 300

Query: 301 TEQSDVGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 354
            + SDVGLM DLVMMAHTN GKERT +EWA++I+  GF+  T+T IQAIQS+I+C P
Sbjct: 301 KKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 357

BLAST of CmaCh11G013560 vs. TrEMBL
Match: A0A0A0K2N4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017720 PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 1.9e-119
Identity = 216/349 (61.89%), Postives = 268/349 (76.79%), Query Frame = 1

Query: 6   KETLSKKEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLHC 65
           K    K+EEE +A +++WKYIFGF EMA +KCAIEL+I D IE HGSPMTL QLS+ L+C
Sbjct: 7   KSIPKKQEEEEEARVEIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQLSTALNC 66

Query: 66  SSPLLHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEASPAML 125
           S+ LL+RILRFLV RGIFK+     ++I Y QTPLSRLLA+ N +S+AP +LLE+SP ML
Sbjct: 67  SASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMAPFLLLESSPVML 126

Query: 126 AAWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTG-LVVAAILDGC 185
           A WH LSA +K  G  PFEAAHG DVW +AAA+P HN +IN+AM C+   L V AIL+ C
Sbjct: 127 APWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEEC 186

Query: 186 QGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMFD 245
             IF+G+GSLVDVGGGNG  LS++VKA  WI+GINFDLPHVIS+S  Y  VEHV G+M D
Sbjct: 187 PQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHVGGNMLD 246

Query: 246 FVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQS---D 305
            +P ADAAFI W LHDW+DE CI ILKNCK AI +  GKVIIV+A+I+E+ E   +   D
Sbjct: 247 SIPKADAAFIMWVLHDWDDETCIKILKNCKGAISEKRGKVIIVEALIEERSEENNNKLGD 306

Query: 306 VGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIE 351
           VGLMLD+VMMAHT  GKERT KEW +V++  GF+++T+T I+A+ SVI+
Sbjct: 307 VGLMLDMVMMAHTKNGKERTSKEWGHVLHQAGFTQYTITPIRAVHSVIQ 355

BLAST of CmaCh11G013560 vs. TrEMBL
Match: A0A0A0K0Q2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G018720 PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.6e-118
Identity = 221/351 (62.96%), Postives = 263/351 (74.93%), Query Frame = 1

Query: 9   LSKKEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLHCSSP 68
           L  +EE+    +Q+WKYIFGF EMA IKCAIEL+I D+IE HG  MTL QLSS+L+CS  
Sbjct: 12  LKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLSQLSSSLNCSPL 71

Query: 69  LLHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEASPAMLAAW 128
           LLHRILRFLV RGIFKE   +     Y+ TP+SRLL T   +S+APL LLE+SP M+A W
Sbjct: 72  LLHRILRFLVHRGIFKEEKTTKC---YSHTPMSRLLTTTGPNSMAPLHLLESSPVMIAPW 131

Query: 129 HNLSAYVKSG----GRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVA-AILDG 188
           HNLSA VK+     G  PFE AHGMD+W YAAANP H+ LINE+M C   LV+  A+L+ 
Sbjct: 132 HNLSACVKASDHENGSQPFEMAHGMDLWTYAAANPSHSSLINESMACFARLVILPAVLER 191

Query: 189 CQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMF 248
           C  IFDGVG LVDVGGGNG  LS++VKAC WI+GINFDLPHV+S S  YE ++HV G+MF
Sbjct: 192 CGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGIQHVGGNMF 251

Query: 249 DFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEE-TEQSDV 308
           D VP ADAAFI   LHDW+DEECI ILKNCKEAIP+ TGKVIIV+ VIDEKEE ++ SD 
Sbjct: 252 DSVPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKEEISKYSDA 311

Query: 309 GLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 354
            LM+D++MMAHT  GKERT +EWA V+   GFS +T+T    IQSVI+  P
Sbjct: 312 RLMMDMIMMAHTIKGKERTNEEWANVLQKAGFSYYTITPTSTIQSVIQAFP 359

BLAST of CmaCh11G013560 vs. TrEMBL
Match: A0A061DXX0_THECC (RS-norcoclaurine 6-O-methyltransferase OS=Theobroma cacao GN=TCM_006540 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 1.9e-114
Identity = 201/340 (59.12%), Postives = 254/340 (74.71%), Query Frame = 1

Query: 14  EEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLHCSSPLLHRI 73
           E+ +A I +W+YIFGF EMA +KCAIEL IAD IE+HGSPMTL +LS+ L C    LHRI
Sbjct: 14  EDAEAAIDVWRYIFGFVEMAVVKCAIELGIADAIENHGSPMTLSELSAALGCEPSRLHRI 73

Query: 74  LRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEASPAMLAAWHNLSA 133
           +RF+V   +FKE  IS   IGYAQTPLSR L +   +S+A L+LLE+SP MLA WH+L A
Sbjct: 74  MRFMVHNQVFKEEPISQGSIGYAQTPLSRRLTSSGVNSMAALVLLESSPVMLAPWHSLRA 133

Query: 134 YVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAAILDGCQGIFDGVGS 193
            V + G  PFEAAHG D+W YA ANP H+ LINEAM C+  + V AI++GC  +FDG+GS
Sbjct: 134 RVLASGDSPFEAAHGKDIWSYAEANPGHSTLINEAMACAARVSVPAIIEGCPEVFDGLGS 193

Query: 194 LVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMFDFVPNADAAF 253
           LVDVGGGNG  L+ +VKA  WIRGINFDLPHV++ +  ++ +E+V GDMF+ +P ADAAF
Sbjct: 194 LVDVGGGNGTALTTLVKAFPWIRGINFDLPHVVAAATQFDGIENVGGDMFESIPKADAAF 253

Query: 254 IKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQSDVGLMLDLVMMAH 313
             W LHDW+DEEC  ILK C+EAIP++ GKVIIV+AV+++  + +   + LMLD+VMMAH
Sbjct: 254 FMWVLHDWDDEECTKILKKCREAIPEDKGKVIIVEAVLEDDTDDKLDFLRLMLDMVMMAH 313

Query: 314 TNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 354
           TN GKERT KEW YV+   GF+R  V  I+A+QSVIE  P
Sbjct: 314 TNKGKERTLKEWRYVLGHAGFTRINVKRIRAVQSVIEAYP 353

BLAST of CmaCh11G013560 vs. TrEMBL
Match: A0A161ZU23_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_022415 PE=4 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 9.2e-114
Identity = 201/349 (57.59%), Postives = 254/349 (72.78%), Query Frame = 1

Query: 7   ETLSKKEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLHCS 66
           E  +  +E  +A + +W+++FGF+EMA +KCAIEL I +I+E  G PMTL +LSS L CS
Sbjct: 8   EVTNGNDETVEAQVDIWRFVFGFTEMALVKCAIELGIPEILEKQGDPMTLSELSSALSCS 67

Query: 67  SPLLHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEASPAMLA 126
           SP L RI+RFL++RGIFKE       +GY QTPLSRLL     +SLA  +L E+S  MLA
Sbjct: 68  SPALFRIMRFLMNRGIFKEKITEQGALGYVQTPLSRLLIKDGRNSLATFLLFESSSVMLA 127

Query: 127 AWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAAILDGCQG 186
            WH LSA V   G   F+ AHG  VW YAA NP H++ I+EAM C T L V A+LDGC  
Sbjct: 128 PWHFLSARVLDDGTSAFDCAHGKSVWEYAAENPGHSKFIDEAMACDTRLTVPAVLDGCPE 187

Query: 187 IFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMFDFV 246
           +F G+ S+VDVGGGNG  LS+++KAC WIRGINFD+PHV++ +P YE +EHV GDMF  V
Sbjct: 188 VFTGLSSIVDVGGGNGTALSILIKACPWIRGINFDVPHVLAVAPEYEGIEHVGGDMFVSV 247

Query: 247 PNADAAFIKWTLHDWNDEECITILKNCKEAIPK--NTGKVIIVDAVIDEKEETEQSDVGL 306
           P A+A FIKW LHDWND+ECI ILKNC+EAI +    GKVIIV+AVI++K+  +  DVGL
Sbjct: 248 PKANATFIKWVLHDWNDDECIQILKNCREAISEYGTAGKVIIVEAVIEDKKGGKLKDVGL 307

Query: 307 MLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 354
           MLD+VM+AHT+ GKERT +EWAYV+   GF+R T+  IQA+ SVIE  P
Sbjct: 308 MLDMVMLAHTSKGKERTAEEWAYVLREAGFTRHTIKNIQAVSSVIEAYP 356

BLAST of CmaCh11G013560 vs. TAIR10
Match: AT4G35160.1 (AT4G35160.1 O-methyltransferase family protein)

HSP 1 Score: 353.2 bits (905), Expect = 1.8e-97
Identity = 186/368 (50.54%), Postives = 245/368 (66.58%), Query Frame = 1

Query: 1   MERTDKETLSKK--EEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGS--PMTL 60
           ++R   E   +K  +EE +A + +WKY+FGF+++AA KCAI+L+I + IE+H S  P+TL
Sbjct: 10  LDRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTL 69

Query: 61  PQLSSTLHCSSPLLHRILRFLVSRGIFKEYSISDDLI-GYAQTPLSR--LLATKNGSSLA 120
            +LSS +  S   L RI+RFLV +GIFKE    D L  GY  TPLSR  ++  ++G SLA
Sbjct: 70  AELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLA 129

Query: 121 PLMLLEASPAMLAAWHNLSAYVKSGGRF----PFEAAHGMDVWRYAAANPKHNRLINEAM 180
           P +L E +P MLA W  LS+ V S        PF+A HG DVW +A  NP  + +INEAM
Sbjct: 130 PFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAM 189

Query: 181 GCSTGLVVAAILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTS 240
            C    VV  +   C G+FDGV ++VDVGGG G T+ ++VK   WI+G NFDLPHVI  +
Sbjct: 190 ACDARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVA 249

Query: 241 PVYERVEHVAGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDA 300
            V + VE+V GDMFD +P  DA FIKW LHDW D++CI ILKNCKEA+P N GKV+IV++
Sbjct: 250 EVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVES 309

Query: 301 VIDEKEETEQSD--------VGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTC 350
           VI E ++T   D        V LMLD+VMMAHT+TGKERT KEW +V+   GF+R+ V  
Sbjct: 310 VIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRD 369

BLAST of CmaCh11G013560 vs. TAIR10
Match: AT4G35150.1 (AT4G35150.1 O-methyltransferase family protein)

HSP 1 Score: 280.0 bits (715), Expect = 2.0e-75
Identity = 158/359 (44.01%), Postives = 213/359 (59.33%), Query Frame = 1

Query: 1   MERTDKETLSKKEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGS--PMTLPQ 60
           ME + +  L   +EE +A + +W+Y+FGF+++AA KCAI+L+I + IE+H S  P+TL +
Sbjct: 1   MEESKRNLL---DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSE 60

Query: 61  LSSTLHCSSPLLHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLL 120
           LSS +  S   L RI+RFL          +   L  + + P    LAT  G +  PL   
Sbjct: 61  LSSAVSASPSHLRRIMRFL----------VHQGL--FKEVPTKDGLAT--GYTNTPL--- 120

Query: 121 EASPAMLAAWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVA 180
                                R      HG D+W +A  N  H++LINEAM C    VV 
Sbjct: 121 -------------------SRRMMITKLHGKDLWAFAQDNLCHSQLINEAMACDARRVVP 180

Query: 181 AILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHV 240
            +   CQG+FDGV ++VDVGGG G T+ ++VK   WI+G NFDLPHVI  + V + VE+V
Sbjct: 181 RVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENV 240

Query: 241 AGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEET- 300
            GDMFD +P +DA  IKW LHDW D++CI ILKNCKEA+  N GKV+IV+ VI EK+ T 
Sbjct: 241 EGDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTM 300

Query: 301 ---EQSD----VGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVI 350
              E+ D    V L LD+VMM HT+TGKERT KEW +V+   GF+R+ V     +QS+I
Sbjct: 301 IAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLI 320

BLAST of CmaCh11G013560 vs. TAIR10
Match: AT5G54160.1 (AT5G54160.1 O-methyltransferase 1)

HSP 1 Score: 219.5 bits (558), Expect = 3.2e-57
Identity = 124/319 (38.87%), Postives = 189/319 (59.25%), Query Frame = 1

Query: 34  AIKCAIELQIADIIEDHGSPMTLPQLSSTLHCSSP----LLHRILRFLVSRGIF--KEYS 93
           A+K A+EL + +I+  +GSPM+  +++S L   +P    +L RILR L S  +       
Sbjct: 35  ALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRK 94

Query: 94  ISDDLIG--YAQTPLSRLLATKN--GSSLAPLMLLEASPAMLAAWHNLSAYVKSGGRFPF 153
           +S D +   Y   P+ + L TKN  G S+A L L+     ++ +W++L   +  GG  PF
Sbjct: 95  LSGDGVERIYGLGPVCKYL-TKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGG-IPF 154

Query: 154 EAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAAILDGCQGIFDGVGSLVDVGGGNGA 213
             A+GM  + Y   +P+ N++ N  M   + + +  IL+  +G F+G+ SLVDVGGG GA
Sbjct: 155 NKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGGIGA 214

Query: 214 TLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMFDFVPNADAAFIKWTLHDWND 273
           TL ++V     ++GINFDLPHVI  +P +  +EHV GDMF  VP  DA F+KW  HDW+D
Sbjct: 215 TLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSD 274

Query: 274 EECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQSDVGLM-LDLVMMAHTNTGKERTR 333
           E C+  LKNC E++P++ GKVI+ + ++ E  ++  S   ++ +D +M+AH   GKERT 
Sbjct: 275 EHCVKFLKNCYESLPED-GKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTE 334

Query: 334 KEWAYVINAVGFSRFTVTC 342
           KE+  +  A GF    V C
Sbjct: 335 KEFEALAKASGFKGIKVVC 349

BLAST of CmaCh11G013560 vs. TAIR10
Match: AT1G77520.1 (AT1G77520.1 O-methyltransferase family protein)

HSP 1 Score: 188.7 bits (478), Expect = 6.0e-48
Identity = 131/370 (35.41%), Postives = 189/370 (51.08%), Query Frame = 1

Query: 6   KETLSKKEEE---GQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSST 65
           K  L+K+E+E       +Q    +   +    +K A EL + D I   G+   L      
Sbjct: 14  KPVLTKEEQEVDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCE-- 73

Query: 66  LHCSSP----------LLHRILRFLVSRGIFKEYSISDDLIG--------YAQTPLSR-L 125
           + CS P          LL R+L  LVS  I K   I     G        YA  P+ +  
Sbjct: 74  IACSLPTKPTNPEAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYF 133

Query: 126 LATKNGS-SLAPLMLLEASPAMLAAWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHN 185
           L   +G+ SL PL +L  +      W NL   +  G R  F +AHGM ++ Y  ++    
Sbjct: 134 LRDSDGTGSLVPLFMLLHTQVFFKTWTNLKDVILEG-RDAFNSAHGMKIFEYINSDQPFA 193

Query: 186 RLINEAMGCSTGLVVAAILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDL 245
            L N AM   + +++  +LD  +G F+ V +LVDVGGGNG  L +V      I+G+NFDL
Sbjct: 194 ELFNRAMSEPSTMIMKKVLDVYRG-FEDVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDL 253

Query: 246 PHVISTSPVYERVEHVAGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTG 305
             V++ +P Y  VEHV+GDMF  VP  DA F+KW LHDW DE+CI ILKNC +++P+  G
Sbjct: 254 AQVLTQAPFYPGVEHVSGDMFVEVPKGDAVFMKWILHDWGDEDCIKILKNCWKSLPEK-G 313

Query: 306 KVIIVDAVI--DEKEETEQSDVGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVT 351
           K+IIV+ V   + K     S+    +DL+M+   + GKER+  ++  +  A GF R  + 
Sbjct: 314 KIIIVEFVTPKEPKGGDLSSNTVFAMDLLMLTQCSGGKERSLSQFENLAFASGFLRCEII 373

BLAST of CmaCh11G013560 vs. TAIR10
Match: AT1G51990.1 (AT1G51990.1 O-methyltransferase family protein)

HSP 1 Score: 187.2 bits (474), Expect = 1.7e-47
Identity = 108/315 (34.29%), Postives = 179/315 (56.83%), Query Frame = 1

Query: 35  IKCAIELQIADIIE------DHGSPMTLPQLSSTLHCSSPLL-HRILRFLVSRGIFKEYS 94
           +K A EL + +I+        + SP+ L  +++  +  +P++  R+LRFLV+  +     
Sbjct: 32  VKTARELDLFEIMAKARPLGSYLSPVDLASMAAPKNPHAPMMIDRLLRFLVAYSVCTCKL 91

Query: 95  ISDD------LIGYAQTPLSRLLATKNGSSLAPLMLLEASPAMLAAWHNLSAYVKSGGRF 154
           + D+        G  +    +L+  ++G S+AP +L   + A    W  L+  ++ GG  
Sbjct: 92  VKDEEGRESRAYGLGKVG-KKLIKDEDGFSIAPYVLAGCTKAKGGVWSYLTEAIQEGGAS 151

Query: 155 PFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAAILDGCQGIFDGVGSLVDVGGGN 214
            +E A+   ++ Y   N    ++ NE+M   T +V+  IL+   G F+GV   VDVGG  
Sbjct: 152 AWERANEALIFEYMKKNENLKKIFNESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGSL 211

Query: 215 GATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMFDFVPNADAAFIKWTLHDW 274
           G+ L+ ++     I+GINFDLPH++  +P    VEH+ GDMFD +P  +   +KW LHDW
Sbjct: 212 GSNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWILHDW 271

Query: 275 NDEECITILKNCKEAIPKNTGKVIIVDAVID-EKEETE-QSDVGLMLDLVMMAHTNTGKE 334
           NDE+C+ ILKNCK+A+P+ TG++I+++ ++  E  ET+  +   L  DL MM+ T+ GKE
Sbjct: 272 NDEKCVEILKNCKKALPE-TGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGGKE 331

BLAST of CmaCh11G013560 vs. NCBI nr
Match: gi|449470501|ref|XP_004152955.1| (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 487.3 bits (1253), Expect = 2.3e-134
Identity = 233/357 (65.27%), Postives = 291/357 (81.51%), Query Frame = 1

Query: 1   MERTDKETLSKKEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLS 60
           ME   KE+ S+KEE+GQAIIQMW+YIF F+EMAAIKC ++L+IADIIE +GSP+TL QLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLHCSSPLLHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEA 120
           STL+CSS LLHRILRFL+ RGIFK+ +I ++ IGY+QTP+SRLLAT   +S+APL+LLE 
Sbjct: 61  STLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLET 120

Query: 121 SPAMLAAWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAAI 180
           SP MLA W +LS ++K+    PFE AHG D+WRYA AN +HN L NEAM C   ++V+AI
Sbjct: 121 SPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSAI 180

Query: 181 LDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAG 240
           ++GC  +FDGV  LVDVGGGNG+TLS++VKA  W++GINFDLPHV+  SP YE VEHVAG
Sbjct: 181 IEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVAG 240

Query: 241 DMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVID----EKEE 300
           +MFDFVPNAD AF+KW LHDW+DEECI ILK CKE+IPK+ GKVII++A+I+    EK+ 
Sbjct: 241 NMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKKN 300

Query: 301 TEQSDVGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 354
            + SDVGLM DLVMMAHTN GKERT +EWA++I+  GF+  T+T IQAIQS+I+C P
Sbjct: 301 KKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 357

BLAST of CmaCh11G013560 vs. NCBI nr
Match: gi|659094136|ref|XP_008447904.1| (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 487.3 bits (1253), Expect = 2.3e-134
Identity = 234/358 (65.36%), Postives = 292/358 (81.56%), Query Frame = 1

Query: 1   MERTDKETLSKKEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLS 60
           ME   KE+ S+KEE+GQAIIQMW+YIF F+EMAAIKCAI+L+IADIIE +GSP+TL QLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCAIDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLHCSSPLLHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEA 120
           STL+CSS LL+RILRFL+ RGIFK  +I ++ IGY+QTP+SRLLA    +S+APL+LLE 
Sbjct: 61  STLNCSSSLLYRILRFLIHRGIFKRETIDENQIGYSQTPMSRLLAINVENSMAPLLLLET 120

Query: 121 SPAMLAAWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAAI 180
           SP MLA W +LSA++K+    PFE AHG D+W YA AN +HN L NEAM CS  ++V+AI
Sbjct: 121 SPVMLAPWQHLSAHLKNSDTSPFEIAHGKDLWNYAEANHEHNLLFNEAMACSAKVIVSAI 180

Query: 181 LDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAG 240
           ++GC  +FDGVG LVDVGGGNG+TL+++VK C W++GINFDLPHV+  SP YE VEHVAG
Sbjct: 181 IEGCGDVFDGVGCLVDVGGGNGSTLNILVKVCPWMKGINFDLPHVVCASPQYENVEHVAG 240

Query: 241 DMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ- 300
           +MFDFVP+AD AF+KW LHDW DEECI ILK CKEAIPK+ GKVII++A+I E E+ E+ 
Sbjct: 241 NMFDFVPHADVAFLKWILHDWEDEECIKILKKCKEAIPKSGGKVIIIEAIIIEAEKGEKM 300

Query: 301 ----SDVGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 354
               SDVGLM DLVMMAHTN G+ERT +EWA++I+  GF+R T+T +QAIQS+I+C P
Sbjct: 301 KKKLSDVGLMFDLVMMAHTNKGRERTAEEWAFLIHQAGFTRHTITPLQAIQSLIQCFP 358

BLAST of CmaCh11G013560 vs. NCBI nr
Match: gi|659094140|ref|XP_008447906.1| (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 444.5 bits (1142), Expect = 1.7e-121
Identity = 221/349 (63.32%), Postives = 271/349 (77.65%), Query Frame = 1

Query: 8   TLSKKEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLHCSS 67
           ++SKKEEE +A +Q+WKYIFGF EMA +KCAIEL+I D IE HGSPMTL QLS+ L+CSS
Sbjct: 8   SISKKEEE-EARVQIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQLSTALNCSS 67

Query: 68  PLLHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEASPAMLAA 127
            LL+RILRFLV RGIFK+    +++I Y  TPLSRLLA+ N +S+APL+LLE+SP MLA 
Sbjct: 68  SLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMAPLLLLESSPVMLAP 127

Query: 128 WHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGL-VVAAILDGCQG 187
           WH LSA +K  G  PFEAAHG DVW +AAA+P HN +IN+AM C+  +  V AIL+ C  
Sbjct: 128 WHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVHTVPAILEDCPQ 187

Query: 188 IFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMFDFV 247
           IF+G+GSLVDVGGGNG  LS++VKA  WI+GINFDLPHVIS+S  Y  VEHV G+M D +
Sbjct: 188 IFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHVGGNMLDSI 247

Query: 248 PNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQS-----D 307
           P ADAAFI W LHDW+DE CI ILKNCKEAI +  GKVIIV+A+I+E+ E E +     D
Sbjct: 248 PKADAAFIMWVLHDWDDETCIKILKNCKEAISEKRGKVIIVEAIIEERSEEENNNNNLGD 307

Query: 308 VGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIE 351
           VGLMLD+VMMAHT  GKERT KEW  V+   GF+R+T+T I+A+ SVI+
Sbjct: 308 VGLMLDMVMMAHTENGKERTIKEWGNVLRQAGFTRYTITPIRAVHSVIQ 355

BLAST of CmaCh11G013560 vs. NCBI nr
Match: gi|449454368|ref|XP_004144927.1| (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 437.2 bits (1123), Expect = 2.8e-119
Identity = 216/349 (61.89%), Postives = 268/349 (76.79%), Query Frame = 1

Query: 6   KETLSKKEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLHC 65
           K    K+EEE +A +++WKYIFGF EMA +KCAIEL+I D IE HGSPMTL QLS+ L+C
Sbjct: 7   KSIPKKQEEEEEARVEIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQLSTALNC 66

Query: 66  SSPLLHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEASPAML 125
           S+ LL+RILRFLV RGIFK+     ++I Y QTPLSRLLA+ N +S+AP +LLE+SP ML
Sbjct: 67  SASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMAPFLLLESSPVML 126

Query: 126 AAWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTG-LVVAAILDGC 185
           A WH LSA +K  G  PFEAAHG DVW +AAA+P HN +IN+AM C+   L V AIL+ C
Sbjct: 127 APWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEEC 186

Query: 186 QGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMFD 245
             IF+G+GSLVDVGGGNG  LS++VKA  WI+GINFDLPHVIS+S  Y  VEHV G+M D
Sbjct: 187 PQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHVGGNMLD 246

Query: 246 FVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQS---D 305
            +P ADAAFI W LHDW+DE CI ILKNCK AI +  GKVIIV+A+I+E+ E   +   D
Sbjct: 247 SIPKADAAFIMWVLHDWDDETCIKILKNCKGAISEKRGKVIIVEALIEERSEENNNKLGD 306

Query: 306 VGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIE 351
           VGLMLD+VMMAHT  GKERT KEW +V++  GF+++T+T I+A+ SVI+
Sbjct: 307 VGLMLDMVMMAHTKNGKERTSKEWGHVLHQAGFTQYTITPIRAVHSVIQ 355

BLAST of CmaCh11G013560 vs. NCBI nr
Match: gi|449454255|ref|XP_004144871.1| (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 434.1 bits (1115), Expect = 2.3e-118
Identity = 221/351 (62.96%), Postives = 263/351 (74.93%), Query Frame = 1

Query: 9   LSKKEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLHCSSP 68
           L  +EE+    +Q+WKYIFGF EMA IKCAIEL+I D+IE HG  MTL QLSS+L+CS  
Sbjct: 12  LKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLSQLSSSLNCSPL 71

Query: 69  LLHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLEASPAMLAAW 128
           LLHRILRFLV RGIFKE   +     Y+ TP+SRLL T   +S+APL LLE+SP M+A W
Sbjct: 72  LLHRILRFLVHRGIFKEEKTTKC---YSHTPMSRLLTTTGPNSMAPLHLLESSPVMIAPW 131

Query: 129 HNLSAYVKSG----GRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVA-AILDG 188
           HNLSA VK+     G  PFE AHGMD+W YAAANP H+ LINE+M C   LV+  A+L+ 
Sbjct: 132 HNLSACVKASDHENGSQPFEMAHGMDLWTYAAANPSHSSLINESMACFARLVILPAVLER 191

Query: 189 CQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVAGDMF 248
           C  IFDGVG LVDVGGGNG  LS++VKAC WI+GINFDLPHV+S S  YE ++HV G+MF
Sbjct: 192 CGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGIQHVGGNMF 251

Query: 249 DFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEE-TEQSDV 308
           D VP ADAAFI   LHDW+DEECI ILKNCKEAIP+ TGKVIIV+ VIDEKEE ++ SD 
Sbjct: 252 DSVPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKEEISKYSDA 311

Query: 309 GLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 354
            LM+D++MMAHT  GKERT +EWA V+   GFS +T+T    IQSVI+  P
Sbjct: 312 RLMMDMIMMAHTIKGKERTNEEWANVLQKAGFSYYTITPTSTIQSVIQAFP 359