Lsi11G012330 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi11G012330
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionO-methyltransferase, putative
Locationchr11 : 20917988 .. 20922071 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTTTGCTCTCAAAGAATCCATACCAAAGAATGAAGAAGATGAAAATGCTCGAGTTCAAATTTGGAAATACATATTTGGTTTTGTGGAAATGGCAATTGTAAAATGTGCCATAGAACTCAAAATTGCTGATACAATCGAAAGCCATGGAAGTTCAATGACACTCTCCCAATTATCCTCAGCTTTAAACTGTTCTTCATCACTTCTCTACCGCATCTTGAGATTCTTAGTCCATCGTGGAATTTTCAAAGAAGAAATCACTGAAGAAAATCTCACAAGCTATGCTCAAACACCTTTGTCTAGGCTGCTTGCAAGTAGCAACAACAACAGCATGGCTCCATTTCTTTTATTGGAGAGCAACCCAGTAATGCTGGCACCATGGCATGGCCTCAGTGCTCGCATTAAAGGCACTGGAGAAACCCCATTTGAGGCTGCTCATGGCAAAGATGTATGGAGCTTTGCTGAAGCTGACCCAATACATAACACTGTAATCAATGATGCTATGTCCTGTACTGCAAGGGTTCTTACTGTGCCTGCAATACTTGAAGATTGTCCAGAAATTTTTGAAGGAGTTGGAAGTTTAGTGGATGTAGGAGGAGGAAATGGAACTAGCTTGAGCATGATAGTCAAAGCTTGTCCTTGGATTAAAGGCATCAACTTTGACCTTCCACATGTCATTTCTTCTTCTCAAGAATACATTGGTGTTCAACATGTTGGTGGGAACATGTTTGATTCCATCCCTCAGGCTGATGCAGCTTTCTTCATGGTTAGTATACACATATAAAATGGCTAAATTTAGTTTAGTCATCCTAGGACTGTAAATCTGTGTTTAATATTTGGTCAACGAATATTCAGAAGTGTCTAATAGATCTCTAAAATTTCTATTTTATGTTTAATAAGTCCTTGATTTTAGAAAGTGTCTAATAGATTCTAAAATTTAATTTTCTGGTTTGTGTATCAGGTTCGGATTTCAAAAAAGTGTCTAATAGGTCATTAAACTTTTAAGTTTCTATCTAAAAGTTCCGTGAAATTTCAATGTTGAGAGTCAACTTTTAATAGTCAATGTTGGACAATCTATGTTCGGGGTTTATTAAAATTCACAAACTTACTAGGCACTGAATGGAGATTAAAACTCAGGAACTTTNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTCGTGGTAGGTCACGAAAATAAAATTAGAAGTTTAGAAATTACTTATCAAACACCTTTTCTTTTCAATACTAAATAAACACGAATCTTGTTTAGAGATTAAACTTATAATTTAAGGTTATTTTTTTTCTCTCCATCCCCATCTCTATATATGGATGTGAAAGAACAAAAAGGTATAGCATCCAAATATAGAAAAAGATAGAATCCCAAATGGACTTTGCTCTCAAAGAATCCATACCAAAGAATGAAGAAGATGAAAATGCTCGAGTTCAAATTTGGAAATACATATTTGGTTTTGTGGAAATGGCAATTGTAAAATGTGCCATAGAACTCAAAATTGCTGATACAATCGAAAGCCATGGAAGTTCAATGACACTCTCCCAATTATCCTCAGCTTTAAACTGTTCTTCATCACTTCTCTACCGCATCTTGAGATTCTTAGTCCATCGTGGAATTTTCAAAGAAGAAATCACTGAAGAAAATCTCACAAGCTATGCTCAAACACCTTTGTCTAGGCTGCTTGCAAGTAGCAACAACAACAGCATGGCTCCATTTCTTTTATTGGAGAGCAACCCAGTAATGCTGGCACCATGGCATGGCCTCAGTGCTCGCATTAAAGGCACTGGAGAAACCCCATTTGAGGCTGCTCATGGCAAAGATGTATGGAGCTTTGCTGAAGCTGACCCAATACATAACACTGTAATCAATGATGCTATGTCCTGTACTGCAAGGGTTCTTACTGTGCCTGCAATACTTGAAGATTGTCCAGAAATTTTTGAAGGAGTTGGAAGTTTAGTGGATGTAGGAGGAGGAAATGGAACTAGCTTGAGCATGATAGTCAAAGCTTGTCCTTGGATTAAAGGCATCAACTTTGACCTTCCACATGTCATTTCTTCTTCTCAAGAATACATTGGTGTTCAACATGTTGGTGGGAACATGTTTGATTCCATCCCTCAGGCTGATGCAGCTTTCTTCATGGTTAGTATACACATATAAAATGGCTAAATTTAGTTTAGTCATCCTAGGACTGTAAATCTGTGTTTAATATTTGGTCAACGAATATTCAGAAGTGTCTAATAGATCTCTAAAATTTCTATTTTATGTTTAATAAGTCCTTGATTTTAGAAAGTGTCTAATAGATTCTAAAATTTAATTTTCTGGTTTGTGTATCAGGTTCGGATTTCAAAAAAGTGTCTAATAGGTCATTAAACTTTTAAGTTTCTATCTAAAAGTTCCGTGAAATTTCAATGTTGAGAGTCAACTTTTAATAGTCAATGTTGGACAATCTATGTTCGGGGTTTATTAAAATTCACAAACTTACTAGGCACTGAATGGAGATTAAAACTCAGGAACTTTTAGGCATGAAATTGAAGATTTAGAGATCTAGTTTTTTTTTTAAAAAAAAGATATTTTGGGTCAGGGGGTGCACCCGATCATCTCCACTAGGTGGACACCATCTTAGCACCCTCATCGTCTTCCTTTCATTGATATTCAATAAGCACCAAGAGAGAAGTACAGTGTAGGGCTAGAGGAAAGCCCACATAAATGGAAAAGACTAGCTTTTACAAAAGGCCTGTAGATTTAAGGCAATAGTAGATGGGTTGTAATCTTTGAAAAGAGTGTCTCTATAGGACCAACAGCCCGTGAGGGAACAAATATCTTCCCATAGGTTCGTTGTGCTTCTTACCATTTATGGAACATATTAGATAGGAAAACCTTAATTTATGAGCTTAATTTGTAATTTAACCTAAAATATAGGCAAAAAATCACTTCTAGTCCTGGAACGCTTTCATGAATTAACAATTTAGTCCCGATATGTTCAATTTGTAATAATAATTCAATCCTTGAATTTTAATATAGGCAACAATTTAGTCCCTATTACTTTATAATTTGTAACGATTTAGTCCTTATTATGAAAAATATTATTAACGTTTAATGAAATTTCTTACACATGTAGATTGATAAATTGGTCGAAAATCTTTTTTTTATAAACTATAAACATTAAATTATTACATAAGAAAGTTGACACTTAATTTTAAAGATTTTTCCACAGGAGGAACTAAATTAATTATAAATTTCAAAGTATAGCAACCAATTAATTATTACTTATTCAAGCTTAGAGACTAGATTGTTAAAACTTTGACAGAGTATAGGGATTAAGTCGGTACTAACTAAAGTCCAAAAACTAAATTGCTACTTCTATAAAAGCTTAGGAATGAAAAGTGTTTTTTAACCTAAAACATATTAGTAAAGTTTAAATTAACCTCCTATGTTGCTGTTGAAAATATGAAAGGTTTCATTAATAATGAGAAAATAAAAGGTTTTAGCATTAAATTTTATTTATGACTTTTGGTGTGTTTTGTAACTTTAGTGGCTTTTGAGTTCTTTTGGGAGTTTTATTTGTATTAAAAGTTATGAGCATTTAGTAATATTTATGATTTCTTGTGATGTGTTTTGTAAGCTTATAAGGCCGGTTTAGTTAAATATTACTTTCTCCATATTTTTGTCATTCACGTCTTTTTTTCTTCTAACAATTGAATCTATATATGTGCTGAAATTTTTGGAACAAAAAAGAATATTAACTTTTATGAGATTGTCTTATACAGTGGGTTCTACATGATTGGGATGATGAGACATGCATCAAAATTTTGAAGAATTGTAGAGATGCAATTCCAAAAAAGACAGGAAAAGTAATAATTGTAGAAGCAATTATTGAGGAAAAAGAAGAAAATAATATGTCAGACGTTGGATTAATGCTTGACATGGTGATGATGGCTCATACCAACAATGGCAAAGAAAGAACAGCTAAGGAATGGGCTTATGTTCTTCACCAAGCTGGTTTTACCCGACACACCATTACACCCATTCGAGCTGTCCAATCTCTTATTCAAGGTTTCCTTTGA

mRNA sequence

ATGGACTTTGCTCTCAAAGAATCCATACCAAAGAATGAAGAAGATGAAAATGCTCGAGTTCAAATTTGGAAATACATATTTGGTTTTGTGGAAATGGCAATTGTAAAATGTGCCATAGAACTCAAAATTGCTGATACAATCGAAAGCCATGGAAGTTCAATGACACTCTCCCAATTATCCTCAGCTTTAAACTGTTCTTCATCACTTCTCTACCGCATCTTGAGATTCTTAGTCCATCGTGGAATTTTCAAAGAAGAAATCACTGAAGAAAATCTCACAAGCTATGCTCAAACACCTTTGTCTAGGCTGCTTGCAAGTAGCAACAACAACAGCATGGCTCCATTTCTTTTATTGGAGAGCAACCCAGTAATGCTGGCACCATGGCATGGCCTCAGTGCTCGCATTAAAGGCACTGGAGAAACCCCATTTGAGGCTGCTCATGGCAAAGATGTATGGAGCTTTGCTGAAGCTGACCCAATACATAACACTGTAATCAATGATGCTATGTCCTGTACTGCAAGGGTTCTTACTGTGCCTGCAATACTTGAAGATTGTCCAGAAATTTTTGAAGGAGTTGGAAGTTTAGTGGATGTAGGAGGAGGAAATGGAACTAGCTTGAGCATGATAGTCAAAGCTTGTCCTTGGATTAAAGGCATCAACTTTGACCTTCCACATGTCATTTCTTCTTCTCAAGAATACATTGGTGTTCAACATGTTGAATCCCAAATGGACTTTGCTCTCAAAGAATCCATACCAAAGAATGAAGAAGATGAAAATGCTCGAGTTCAAATTTGGAAATACATATTTGGTTTTGTGGAAATGGCAATTGTAAAATGTGCCATAGAACTCAAAATTGCTGATACAATCGAAAGCCATGGAAGTTCAATGACACTCTCCCAATTATCCTCAGCTTTAAACTGTTCTTCATCACTTCTCTACCGCATCTTGAGATTCTTAGTCCATCGTGGAATTTTCAAAGAAGAAATCACTGAAGAAAATCTCACAAGCTATGCTCAAACACCTTTGTCTAGGCTGCTTGCAAGTAGCAACAACAACAGCATGGCTCCATTTCTTTTATTGGAGAGCAACCCAGTAATGCTGGCACCATGGCATGGCCTCAGTGCTCGCATTAAAGGCACTGGAGAAACCCCATTTGAGGCTGCTCATGGCAAAGATGTATGGAGCTTTGCTGAAGCTGACCCAATACATAACACTGTAATCAATGATGCTATGTCCTGTACTGCAAGGGTTCTTACTGTGCCTGCAATACTTGAAGATTGTCCAGAAATTTTTGAAGGAGTTGGAAGTTTAGTGGATGTAGGAGGAGGAAATGGAACTAGCTTGAGCATGATAGTCAAAGCTTGTCCTTGGATTAAAGGCATCAACTTTGACCTTCCACATGTCATTTCTTCTTCTCAAGAATACATTGGTGTTCAACATTGGGTTCTACATGATTGGGATGATGAGACATGCATCAAAATTTTGAAGAATTGTAGAGATGCAATTCCAAAAAAGACAGGAAAAGTAATAATTGTAGAAGCAATTATTGAGGAAAAAGAAGAAAATAATATGTCAGACGTTGGATTAATGCTTGACATGGTGATGATGGCTCATACCAACAATGGCAAAGAAAGAACAGCTAAGGAATGGGCTTATGTTCTTCACCAAGCTGGTTTTACCCGACACACCATTACACCCATTCGAGCTGTCCAATCTCTTATTCAAGGTTTCCTTTGA

Coding sequence (CDS)

ATGGACTTTGCTCTCAAAGAATCCATACCAAAGAATGAAGAAGATGAAAATGCTCGAGTTCAAATTTGGAAATACATATTTGGTTTTGTGGAAATGGCAATTGTAAAATGTGCCATAGAACTCAAAATTGCTGATACAATCGAAAGCCATGGAAGTTCAATGACACTCTCCCAATTATCCTCAGCTTTAAACTGTTCTTCATCACTTCTCTACCGCATCTTGAGATTCTTAGTCCATCGTGGAATTTTCAAAGAAGAAATCACTGAAGAAAATCTCACAAGCTATGCTCAAACACCTTTGTCTAGGCTGCTTGCAAGTAGCAACAACAACAGCATGGCTCCATTTCTTTTATTGGAGAGCAACCCAGTAATGCTGGCACCATGGCATGGCCTCAGTGCTCGCATTAAAGGCACTGGAGAAACCCCATTTGAGGCTGCTCATGGCAAAGATGTATGGAGCTTTGCTGAAGCTGACCCAATACATAACACTGTAATCAATGATGCTATGTCCTGTACTGCAAGGGTTCTTACTGTGCCTGCAATACTTGAAGATTGTCCAGAAATTTTTGAAGGAGTTGGAAGTTTAGTGGATGTAGGAGGAGGAAATGGAACTAGCTTGAGCATGATAGTCAAAGCTTGTCCTTGGATTAAAGGCATCAACTTTGACCTTCCACATGTCATTTCTTCTTCTCAAGAATACATTGGTGTTCAACATGTTGAATCCCAAATGGACTTTGCTCTCAAAGAATCCATACCAAAGAATGAAGAAGATGAAAATGCTCGAGTTCAAATTTGGAAATACATATTTGGTTTTGTGGAAATGGCAATTGTAAAATGTGCCATAGAACTCAAAATTGCTGATACAATCGAAAGCCATGGAAGTTCAATGACACTCTCCCAATTATCCTCAGCTTTAAACTGTTCTTCATCACTTCTCTACCGCATCTTGAGATTCTTAGTCCATCGTGGAATTTTCAAAGAAGAAATCACTGAAGAAAATCTCACAAGCTATGCTCAAACACCTTTGTCTAGGCTGCTTGCAAGTAGCAACAACAACAGCATGGCTCCATTTCTTTTATTGGAGAGCAACCCAGTAATGCTGGCACCATGGCATGGCCTCAGTGCTCGCATTAAAGGCACTGGAGAAACCCCATTTGAGGCTGCTCATGGCAAAGATGTATGGAGCTTTGCTGAAGCTGACCCAATACATAACACTGTAATCAATGATGCTATGTCCTGTACTGCAAGGGTTCTTACTGTGCCTGCAATACTTGAAGATTGTCCAGAAATTTTTGAAGGAGTTGGAAGTTTAGTGGATGTAGGAGGAGGAAATGGAACTAGCTTGAGCATGATAGTCAAAGCTTGTCCTTGGATTAAAGGCATCAACTTTGACCTTCCACATGTCATTTCTTCTTCTCAAGAATACATTGGTGTTCAACATTGGGTTCTACATGATTGGGATGATGAGACATGCATCAAAATTTTGAAGAATTGTAGAGATGCAATTCCAAAAAAGACAGGAAAAGTAATAATTGTAGAAGCAATTATTGAGGAAAAAGAAGAAAATAATATGTCAGACGTTGGATTAATGCTTGACATGGTGATGATGGCTCATACCAACAATGGCAAAGAAAGAACAGCTAAGGAATGGGCTTATGTTCTTCACCAAGCTGGTTTTACCCGACACACCATTACACCCATTCGAGCTGTCCAATCTCTTATTCAAGGTTTCCTTTGA

Protein sequence

MDFALKESIPKNEEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLSSALNCSSSLLYRILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLESNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILEDCPEIFEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQHVESQMDFALKESIPKNEEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLSSALNCSSSLLYRILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLESNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILEDCPEIFEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQHWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEENNMSDVGLMLDMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGFL
BLAST of Lsi11G012330 vs. Swiss-Prot
Match: 7OMT_PAPSO ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum GN=7OMT PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.2e-65
Identity = 140/351 (39.89%), Postives = 204/351 (58.12%), Query Frame = 1

Query: 255 EEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLSSALNCSS----- 314
           EE    + +IW+++F FV+   +KCA+EL I D I SHG  +T+S++  +L  ++     
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64

Query: 315 --SLLYRILRFLVHRGIFKEEITEE-NLTSYAQTPLSRLLASSNNNSMAPFLLLESNPVM 374
               L RI+R LVH+ +F  E+ +E N   Y  T  S+ L   +  +++P +L E+NP++
Sbjct: 65  NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124

Query: 375 LAPWHGLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILED 434
           L PW  L  +      +PFE AHG ++W  A ADP  N  +N AM C+   +    +LE 
Sbjct: 125 LKPWQYLG-KCAQEKSSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLE- 184

Query: 435 CPEIFEGV-GSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH----- 494
             + F G+ GSLVDVGGG G+ ++ IVKA P I+GINFDLPHV++++ E+ GV+H     
Sbjct: 185 YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDM 244

Query: 495 -------------WVLHDWDDETCIKILKNCRDAI-PKKTGKVIIVEAIIEEKEENNMSD 554
                        W+LHDW DE C  ILKNC  AI  KK GKVIIV+ ++     +    
Sbjct: 245 FVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFDK 304

Query: 555 VGLMLDMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGF 578
           +GL+ D++MMAHT  GKERT  EW  +L+ AGF R+ +    A   +I+ F
Sbjct: 305 MGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAF 353

BLAST of Lsi11G012330 vs. Swiss-Prot
Match: 4OMT2_PAPSO (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum GN=4'OMT2 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 8.3e-65
Identity = 127/339 (37.46%), Postives = 203/339 (59.88%), Query Frame = 1

Query: 261 RVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLSSAL---NCSSSLLYRILR 320
           + Q+W  I+GF +  +++CA+E+ IAD I+++  ++TL+QL++ L   N SS  LYR++R
Sbjct: 20  QAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVR 79

Query: 321 FLVHRGIFKEEITEENLTS-YAQTPLSRLLASSNNNSMAPFLLLESNPVMLAPWHGLSAR 380
           +LVH  I ++E     +   Y+  P+  LL      SM P +L  +    +  WH +   
Sbjct: 80  YLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEG 139

Query: 381 IKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILEDCPEIFEGVGS 440
           +     T FE   G D+W + E +P  + + N+ M+   R+LT   ++EDC + F+G+ S
Sbjct: 140 LGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLT-KTLIEDCRDTFQGLDS 199

Query: 441 LVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH---------------- 500
           LVD+GGGNGT++  I +A P IK   +DLPHV+++S +   ++                 
Sbjct: 200 LVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQAIL 259

Query: 501 --WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEENNMSDVGLMLDMVMMAH 560
              +LHDW DE C+ ILK C++AIPK+TGKVIIV+  +EE+  + ++   L+LD+ M+ +
Sbjct: 260 LKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLVN 319

Query: 561 TNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGF 578
           T  G+ERTA +W  +L +AGF  H I PIRA+QS+I+ F
Sbjct: 320 T-GGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAF 356

BLAST of Lsi11G012330 vs. Swiss-Prot
Match: 4OMT1_PAPSO (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 1 OS=Papaver somniferum GN=4'OMT1 PE=2 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 4.6e-63
Identity = 133/346 (38.44%), Postives = 206/346 (59.54%), Query Frame = 1

Query: 254 NEEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLSSAL---NCSSS 313
           +E D   + Q+W  I+G+ +  +++C +E+ IAD I+++  S+TLS+L S L   N +S 
Sbjct: 10  HEVDIKDQAQLWNIIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSD 69

Query: 314 LLYRILRFLVHRGIFKEEITEENLTS-YAQTPLSRLLASSNNNSMAPFLLLESNPVMLAP 373
            LYR+LR+LVH  I  ++     +   Y+  P+  LL   +  SMAP +L  S    L  
Sbjct: 70  NLYRLLRYLVHLNILGQQTCAAGVDRVYSLKPVGTLLLKDSERSMAPVILGLSQKDFLFV 129

Query: 374 WHGLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILEDCPE 433
           W+ +   +     T FE A G D+W + E +P  + + ++  +   R+LT   +L DC +
Sbjct: 130 WNFVKEGLGTGSTTAFEKAMGMDMWKYLEVNPNQSQLFDEGQAGETRLLT-KTLLVDCRD 189

Query: 434 IFEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH--------- 493
            F+G+ SLVDVGGGNGT++  I +A P IK   +DLPHVI++S ++  +           
Sbjct: 190 TFQGMDSLVDVGGGNGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNILKVPGDMFMSV 249

Query: 494 ---------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEENNMSDVGLML 553
                     VLHDW DE C+ ILK C++AIPK+TGKVIIV+  +EE+ E+ ++   L+L
Sbjct: 250 PSAQVLLLKCVLHDWTDEHCVNILKKCKEAIPKETGKVIIVDVALEEESEHELTKARLIL 309

Query: 554 DMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGF 578
           D+ M+ +T  G+ERTA++W  +L +AGF  H I PIRA+QS+I+ F
Sbjct: 310 DIDMLVNT-GGRERTAEDWENLLKRAGFRSHKIRPIRAIQSVIEAF 353

BLAST of Lsi11G012330 vs. Swiss-Prot
Match: 6OMT_COPJA ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.5e-61
Identity = 128/344 (37.21%), Postives = 204/344 (59.30%), Query Frame = 1

Query: 255 EEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLSSALNC---SSSL 314
           +++ +++ ++W +I+GF E  ++KCA++L +A+ I + G+SMTLS+LSS L     +   
Sbjct: 5   KDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNEDA 64

Query: 315 LYRILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLESNPVMLAPWH 374
           LYR++R+LVH  +F +   +  L  Y   P ++ L    +  M   +L  ++   +APWH
Sbjct: 65  LYRVMRYLVHMKLFTKASIDGELR-YGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPWH 124

Query: 375 GLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILEDCPEIF 434
            L   + G   T FE A G ++W +    P  N + N+AM+  +R++ + A++++C  IF
Sbjct: 125 YLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLI-MSALVKECGNIF 184

Query: 435 EGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQ------------ 494
            G+ +LVDVGGG GT++  I  A P IK   +DLPHVI+ S  Y  V             
Sbjct: 185 NGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIPK 244

Query: 495 ------HWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEENNMSDVGLMLDM 554
                   +LHDWDD+ CI+ILK C++A+P K GKVIIV+ ++  + E+  + + L LD+
Sbjct: 245 ADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLDL 304

Query: 555 VMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGF 578
            MM +T  GKERT +EW  ++H AG+  H IT I AVQS+I+ +
Sbjct: 305 DMMLNT-GGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAY 345

BLAST of Lsi11G012330 vs. Swiss-Prot
Match: 4OMT_COPJA (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.4e-59
Identity = 128/344 (37.21%), Postives = 199/344 (57.85%), Query Frame = 1

Query: 257 DENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLSSAL-----NCSSSL 316
           D  A+  +WK I+GF +  +++CA+EL I D I+++   M L+ L+S L     NC +  
Sbjct: 11  DIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDN-- 70

Query: 317 LYRILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLESNPVMLAPWH 376
           LYRILR+LV   I + E +++    YA  P++ LL+ +   SM P +L  +    + PWH
Sbjct: 71  LYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWH 130

Query: 377 GLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILEDCPEIF 436
            +   +   G T FE A G  +W + E  P  + + N+ M+   R+LT  +++    ++F
Sbjct: 131 SMKDGLSDNG-TAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLT-SSLISGSRDMF 190

Query: 437 EGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH----------- 496
           +G+ SLVDVGGGNGT++  I  A P IK   FDLPHVI++S +   ++            
Sbjct: 191 QGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFKSVPS 250

Query: 497 -------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEENNMSDVGLMLDM 556
                   +LHDW+DE  IKILK CR+A+PK  GKVIIV+  ++E+ ++ +S   L+LD+
Sbjct: 251 AQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDI 310

Query: 557 VMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGF 578
            M+ +T  GKERT + W  ++  AGF+   I  I A+QS+I+ F
Sbjct: 311 DMLVNT-GGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVF 349

BLAST of Lsi11G012330 vs. TrEMBL
Match: A0A0A0K2N4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017720 PE=3 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 6.5e-157
Identity = 285/358 (79.61%), Postives = 310/358 (86.59%), Query Frame = 1

Query: 243 MDFALKESIP-KNEEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQL 302
           MDFA ++SIP K EE+E ARV+IWKYIFGFVEMAIVKCAIEL+I DTIESHGS MTLSQL
Sbjct: 1   MDFAPQKSIPKKQEEEEEARVEIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQL 60

Query: 303 SSALNCSSSLLYRILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLE 362
           S+ALNCS+SLLYRILRFLV RGIFK+EI E N+ SY QTPLSRLLASSNNNSMAPFLLLE
Sbjct: 61  STALNCSASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMAPFLLLE 120

Query: 363 SNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVP 422
           S+PVMLAPWH LSARIKG GETPFEAAHGKDVWSFA ADPIHN VINDAMSCTARVLTVP
Sbjct: 121 SSPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVP 180

Query: 423 AILEDCPEIFEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH- 482
           AILE+CP+IFEG+GSLVDVGGGNGT LSMIVKA PWIKGINFDLPHVISSSQ+YIGV+H 
Sbjct: 181 AILEECPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHV 240

Query: 483 -----------------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEE-- 542
                            WVLHDWDDETCIKILKNC+ AI +K GKVIIVEA+IEE+ E  
Sbjct: 241 GGNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKGAISEKRGKVIIVEALIEERSEEN 300

Query: 543 -NNMSDVGLMLDMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGFL 579
            N + DVGLMLDMVMMAHT NGKERT+KEW +VLHQAGFT++TITPIRAV S+IQ FL
Sbjct: 301 NNKLGDVGLMLDMVMMAHTKNGKERTSKEWGHVLHQAGFTQYTITPIRAVHSVIQAFL 358

BLAST of Lsi11G012330 vs. TrEMBL
Match: A0A0A0K0Q2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G018720 PE=3 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 1.3e-120
Identity = 221/346 (63.87%), Postives = 270/346 (78.03%), Query Frame = 1

Query: 255 EEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLSSALNCSSSLLYR 314
           EED + +VQIWKYIFGFVEMA++KCAIELKI D IESHG SMTLSQLSS+LNCS  LL+R
Sbjct: 16  EEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLSQLSSSLNCSPLLLHR 75

Query: 315 ILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLESNPVMLAPWHGLS 374
           ILRFLVHRGIFKEE T +    Y+ TP+SRLL ++  NSMAP  LLES+PVM+APWH LS
Sbjct: 76  ILRFLVHRGIFKEEKTTK---CYSHTPMSRLLTTTGPNSMAPLHLLESSPVMIAPWHNLS 135

Query: 375 ARIKGT----GETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILEDCPEI 434
           A +K +    G  PFE AHG D+W++A A+P H+++IN++M+C AR++ +PA+LE C EI
Sbjct: 136 ACVKASDHENGSQPFEMAHGMDLWTYAAANPSHSSLINESMACFARLVILPAVLERCGEI 195

Query: 435 FEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH---------- 494
           F+GVG LVDVGGGNGT LS++VKACPWIKGINFDLPHV+S S+EY G+QH          
Sbjct: 196 FDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGIQHVGGNMFDSVP 255

Query: 495 --------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEE-NNMSDVGLML 554
                    VLHDWDDE CIKILKNC++AIP+KTGKVIIVE +I+EKEE +  SD  LM+
Sbjct: 256 KADAAFIMEVLHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKEEISKYSDARLMM 315

Query: 555 DMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGF 578
           DM+MMAHT  GKERT +EWA VL +AGF+ +TITP   +QS+IQ F
Sbjct: 316 DMIMMAHTIKGKERTNEEWANVLQKAGFSYYTITPTSTIQSVIQAF 358

BLAST of Lsi11G012330 vs. TrEMBL
Match: A0A0A0K629_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G018730 PE=3 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 1.3e-120
Identity = 216/357 (60.50%), Postives = 274/357 (76.75%), Query Frame = 1

Query: 243 MDFALKESIPKNEEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLS 302
           M+   KES  + EED  A +Q+W+YIF F EMA +KC ++LKIAD IES+GS +TLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS 60

Query: 303 SALNCSSSLLYRILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLES 362
           S LNCSSSLL+RILRFL+HRGIFK+E  +EN   Y+QTP+SRLLA++  NSMAP LLLE+
Sbjct: 61  STLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLET 120

Query: 363 NPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPA 422
           +PVMLAPW  LS  +K +   PFE AHGKD+W +AEA+  HN + N+AM+C A+++ V A
Sbjct: 121 SPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMI-VSA 180

Query: 423 ILEDCPEIFEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH-- 482
           I+E C ++F+GV  LVDVGGGNG++LS++VKA PW+KGINFDLPHV+ +S +Y  V+H  
Sbjct: 181 IIEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVA 240

Query: 483 ----------------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIE----EKE 542
                           W+LHDWDDE CIKILK C+++IPK  GKVII+EAIIE    EK+
Sbjct: 241 GNMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKK 300

Query: 543 ENNMSDVGLMLDMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGF 578
              +SDVGLM D+VMMAHTN GKERTA+EWA+++HQAGFT HTITPI+A+QSLIQ F
Sbjct: 301 NKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCF 356

BLAST of Lsi11G012330 vs. TrEMBL
Match: A0A061DXX0_THECC (RS-norcoclaurine 6-O-methyltransferase OS=Theobroma cacao GN=TCM_006540 PE=3 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 3.8e-117
Identity = 207/340 (60.88%), Postives = 263/340 (77.35%), Query Frame = 1

Query: 256 EDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLSSALNCSSSLLYRI 315
           ED  A + +W+YIFGFVEMA+VKCAIEL IAD IE+HGS MTLS+LS+AL C  S L+RI
Sbjct: 14  EDAEAAIDVWRYIFGFVEMAVVKCAIELGIADAIENHGSPMTLSELSAALGCEPSRLHRI 73

Query: 316 LRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLESNPVMLAPWHGLSA 375
           +RF+VH  +FKEE   +    YAQTPLSR L SS  NSMA  +LLES+PVMLAPWH L A
Sbjct: 74  MRFMVHNQVFKEEPISQGSIGYAQTPLSRRLTSSGVNSMAALVLLESSPVMLAPWHSLRA 133

Query: 376 RIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILEDCPEIFEGVG 435
           R+  +G++PFEAAHGKD+WS+AEA+P H+T+IN+AM+C ARV +VPAI+E CPE+F+G+G
Sbjct: 134 RVLASGDSPFEAAHGKDIWSYAEANPGHSTLINEAMACAARV-SVPAIIEGCPEVFDGLG 193

Query: 436 SLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH--------------- 495
           SLVDVGGGNGT+L+ +VKA PWI+GINFDLPHV++++ ++ G+++               
Sbjct: 194 SLVDVGGGNGTALTTLVKAFPWIRGINFDLPHVVAAATQFDGIENVGGDMFESIPKADAA 253

Query: 496 ---WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEENNMSDVGLMLDMVMMA 555
              WVLHDWDDE C KILK CR+AIP+  GKVIIVEA++E+  ++ +  + LMLDMVMMA
Sbjct: 254 FFMWVLHDWDDEECTKILKKCREAIPEDKGKVIIVEAVLEDDTDDKLDFLRLMLDMVMMA 313

Query: 556 HTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGF 578
           HTN GKERT KEW YVL  AGFTR  +  IRAVQS+I+ +
Sbjct: 314 HTNKGKERTLKEWRYVLGHAGFTRINVKRIRAVQSVIEAY 352

BLAST of Lsi11G012330 vs. TrEMBL
Match: M5W6W6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007692mg PE=3 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 4.0e-114
Identity = 208/347 (59.94%), Postives = 266/347 (76.66%), Query Frame = 1

Query: 253 KNEEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLSSALNCSSSLL 312
           + EE+E+A+V +WKY+FGFVE+A+VKCAIEL IADTIESHGS MTL +LSSAL C  S L
Sbjct: 13  EEEEEEHAKVDVWKYVFGFVEIAVVKCAIELGIADTIESHGSPMTLLELSSALRCDPSPL 72

Query: 313 YRILRFLVHRGIFKEE-ITEENLTSYAQTPLSRLLASSNNNSMAPFLLLESNPVMLAPWH 372
           YRI+R LVH  IFKE+  T+     +AQTPLS+ L  S   SM+  +LLES+PVMLAPWH
Sbjct: 73  YRIMRVLVHLKIFKEKPATQLGPKVFAQTPLSKWLLKSGETSMSALILLESSPVMLAPWH 132

Query: 373 GLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILEDCPEIF 432
           GLSARI+G     FEA HG+DVWS+A+A+P H+ +IN+AM+C ARV  VPA++E C E+F
Sbjct: 133 GLSARIQGISNPAFEAVHGEDVWSYAKANPDHSKLINEAMACDARV-AVPAVIESCLEVF 192

Query: 433 EGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH----------- 492
           +G+ ++VDVGGG+GT+L ++V+ACPW++GINFDLPHV+S +QE   +++           
Sbjct: 193 KGIATIVDVGGGDGTTLRLLVEACPWVQGINFDLPHVVSVAQECDHIKNVGGDMFDSVPK 252

Query: 493 -------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEENN---MSDVGLM 552
                  WVLHDW D+ CI+ILK CR+AIP+  GKVIIVEA+I+EK+E     +++V LM
Sbjct: 253 ADAVIMKWVLHDWGDDECIRILKKCREAIPENKGKVIIVEAVIDEKDEKEDIKLTNVRLM 312

Query: 553 LDMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGF 578
           LDMVMMAHTN GKERT KEW YVL +AGF+RHTITPI AVQS+IQ F
Sbjct: 313 LDMVMMAHTNTGKERTLKEWGYVLGEAGFSRHTITPIHAVQSVIQAF 358

BLAST of Lsi11G012330 vs. TAIR10
Match: AT4G35160.1 (AT4G35160.1 O-methyltransferase family protein)

HSP 1 Score: 328.6 bits (841), Expect = 8.0e-90
Identity = 176/355 (49.58%), Postives = 238/355 (67.04%), Query Frame = 1

Query: 255 EEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSS--MTLSQLSSALNCSSSLL 314
           +E+  A + IWKY+FGF ++A  KCAI+LKI + IE+H SS  +TL++LSSA++ S S L
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHL 83

Query: 315 YRILRFLVHRGIFKEEITEENL-TSYAQTPLSR--LLASSNNNSMAPFLLLESNPVMLAP 374
            RI+RFLVH+GIFKE  T++ L T Y  TPLSR  ++   +  S+APF+L E+ P MLAP
Sbjct: 84  RRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAP 143

Query: 375 WHGLSA----RIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILE 434
           W  LS+     + G+   PF+A HGKDVWSFA+ +P  + +IN+AM+C AR + VP +  
Sbjct: 144 WLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRV-VPRVAG 203

Query: 435 DCPEIFEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH----- 494
            C  +F+GV ++VDVGGG G ++ M+VK  PWIKG NFDLPHVI  ++   GV++     
Sbjct: 204 ACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVEGDM 263

Query: 495 -------------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEENNMSD- 554
                        WVLHDW D+ CIKILKNC++A+P   GKV+IVE++I E ++  + D 
Sbjct: 264 FDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKTMIVDE 323

Query: 555 -------VGLMLDMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLI 575
                  V LMLDMVMMAHT+ GKERT KEW +VL +AGF R+ +  I  VQSLI
Sbjct: 324 RDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLI 377

BLAST of Lsi11G012330 vs. TAIR10
Match: AT4G35150.1 (AT4G35150.1 O-methyltransferase family protein)

HSP 1 Score: 255.0 bits (650), Expect = 1.1e-67
Identity = 147/348 (42.24%), Postives = 204/348 (58.62%), Query Frame = 1

Query: 255 EEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSS--MTLSQLSSALNCSSSLL 314
           +E+  A + IW+Y+FGF ++A  KCAI+LKI + IE+H SS  +TLS+LSSA++ S S L
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSHL 69

Query: 315 YRILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLESNPVMLAPWHG 374
            RI+RFLVH+G             + + P    LA+   N+                   
Sbjct: 70  RRIMRFLVHQG------------LFKEVPTKDGLATGYTNTP------------------ 129

Query: 375 LSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILEDCPEIFE 434
           LS R+  T        HGKD+W+FA+ +  H+ +IN+AM+C AR + VP +   C  +F+
Sbjct: 130 LSRRMMIT------KLHGKDLWAFAQDNLCHSQLINEAMACDARRV-VPRVAGACQGLFD 189

Query: 435 GVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH------------ 494
           GV ++VDVGGG G ++ ++VK  PWIKG NFDLPHVI  +Q   GV++            
Sbjct: 190 GVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIPAS 249

Query: 495 ------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKE--------ENNMSD 554
                 WVLHDW D+ CIKILKNC++A+    GKV+IVE +I EK+        ++ +  
Sbjct: 250 DAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMIAEERDDKLEH 309

Query: 555 VGLMLDMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLI 575
           V L LDMVMM HT+ GKERT KEW +VL +AGF R+ +     VQSLI
Sbjct: 310 VRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLI 320

BLAST of Lsi11G012330 vs. TAIR10
Match: AT5G54160.1 (AT5G54160.1 O-methyltransferase 1)

HSP 1 Score: 163.7 bits (413), Expect = 3.4e-40
Identity = 109/336 (32.44%), Postives = 174/336 (51.79%), Query Frame = 1

Query: 252 PKNEEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLSSALNCSSS- 311
           P    D+ A +   +     V    +K A+EL + + +  +GS M+ ++++S L   +  
Sbjct: 11  PVQVTDDEAALFAMQLASASVLPMALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPE 70

Query: 312 ---LLYRILRFLVHRGIF---KEEITEENLTS-YAQTPLSRLLASSNNN-SMAPFLLLES 371
              +L RILR L    +      +++ + +   Y   P+ + L  + +  S+A   L+  
Sbjct: 71  APVMLDRILRLLTSYSVLTCSNRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQ 130

Query: 372 NPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPA 431
           + V++  W+ L   I   G  PF  A+G   + +   DP  N V N+ MS     +T+  
Sbjct: 131 DKVLMESWYHLKDAILDGG-IPFNKAYGMSAFEYHGTDPRFNKVFNNGMS-NHSTITMKK 190

Query: 432 ILEDCPEIFEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH-- 491
           ILE     FEG+ SLVDVGGG G +L MIV   P +KGINFDLPHVI  +  + G++H  
Sbjct: 191 ILETYKG-FEGLTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVG 250

Query: 492 ----------------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEENNM 551
                           W+ HDW DE C+K LKNC +++P + GKVI+ E I+ E  ++++
Sbjct: 251 GDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLP-EDGKVILAECILPETPDSSL 310

Query: 552 SDVGLM-LDMVMMAHTNNGKERTAKEWAYVLHQAGF 560
           S   ++ +D +M+AH   GKERT KE+  +   +GF
Sbjct: 311 STKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGF 342

BLAST of Lsi11G012330 vs. TAIR10
Match: AT1G77520.1 (AT1G77520.1 O-methyltransferase family protein)

HSP 1 Score: 149.1 bits (375), Expect = 8.6e-36
Identity = 108/338 (31.95%), Postives = 160/338 (47.34%), Query Frame = 1

Query: 276 IVKCAIELKIADTIESHGSSMTLSQLSSALNCSSS--------LLYRILRFLVHRGIFKE 335
           ++K A EL + DTI + G+   LS    A +  +         LL R+L  LV   I K 
Sbjct: 45  VLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNPEAPVLLDRMLSLLVSHSILKC 104

Query: 336 EITEENLTS--------YAQTPLSR--LLASSNNNSMAPFLLLESNPVMLAPWHGLSARI 395
            + E             YA  P+ +  L  S    S+ P  +L    V    W  L   I
Sbjct: 105 RMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPLFMLLHTQVFFKTWTNLKDVI 164

Query: 396 KGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILEDCPEIFEGVGSL 455
              G   F +AHG  ++ +  +D     + N AMS  + ++    +  D    FE V +L
Sbjct: 165 L-EGRDAFNSAHGMKIFEYINSDQPFAELFNRAMSEPSTMIMKKVL--DVYRGFEDVNTL 224

Query: 456 VDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH----------------- 515
           VDVGGGNGT L ++    P IKG+NFDL  V++ +  Y GV+H                 
Sbjct: 225 VDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGDAVFM 284

Query: 516 -WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAII--EEKEENNMSDVGLMLDMVMMA 575
            W+LHDW DE CIKILKNC  ++P+K GK+IIVE +   E K  +  S+    +D++M+ 
Sbjct: 285 KWILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEFVTPKEPKGGDLSSNTVFAMDLLMLT 344

BLAST of Lsi11G012330 vs. TAIR10
Match: AT1G77530.1 (AT1G77530.1 O-methyltransferase family protein)

HSP 1 Score: 146.4 bits (368), Expect = 5.6e-35
Identity = 114/363 (31.40%), Postives = 179/363 (49.31%), Query Frame = 1

Query: 238 HVESQMDFALKESIPKNEEDENARV---QIWKYIFGFVEMAIVKCAIELKIADTIESHGS 297
           H++  +    K  + K E++ + ++   Q    +       ++K A+EL + DTI +  +
Sbjct: 4   HLQDPLTTYPKPGLTKEEQEIDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASN 63

Query: 298 SMTLSQLSSALNCSSS--------LLYRILRFLVHRGIFK---EEITEENLTS-----YA 357
              LS    A++  +         LL R+LR LV   I K    E  E   T      YA
Sbjct: 64  GTWLSPSEIAVSLPNKPTNPEAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYA 123

Query: 358 QTPLSR--LLASSNNNSMAPFLLLESNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSF 417
             P+ +  L  S  + S++  LLL  + V+L  W  L   I   G+  F +AH   ++ +
Sbjct: 124 AEPICKYFLKDSDGSGSLSSLLLLLHSQVILKTWTNLKDVIL-EGKDAFSSAHDMRLFEY 183

Query: 418 AEADPIHNTVINDAMSCTARVLTVPAILEDCPEIFEGVGSLVDVGGGNGTSLSMIVKACP 477
             +D   + + + AMS  +  + +  +LE+    FE V +LVDVGGG GT L +I    P
Sbjct: 184 ISSDDQFSKLFHRAMS-ESSTMVMKKVLEEYRG-FEDVNTLVDVGGGIGTILGLITSKYP 243

Query: 478 WIKGINFDLPHVISSSQEYIGVQH------------------WVLHDWDDETCIKILKNC 537
            IKG+NFDL  V++ +  Y GV+H                  W+LHDW DE CIKILKNC
Sbjct: 244 HIKGVNFDLAQVLTQAPFYPGVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNC 303

Query: 538 RDAIPKKTGKVIIVEAI--IEEKEENNMSDVGLMLDMVMMAHTNNGKERTAKEWAYVLHQ 560
             ++P+K GKVIIVE I  +E K  +   +  L +D++M+   + GKER+  ++  +   
Sbjct: 304 WKSLPEK-GKVIIVEMITPMEPKPNDFSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFA 362

BLAST of Lsi11G012330 vs. NCBI nr
Match: gi|449454368|ref|XP_004144927.1| (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 562.4 bits (1448), Expect = 9.3e-157
Identity = 285/358 (79.61%), Postives = 310/358 (86.59%), Query Frame = 1

Query: 243 MDFALKESIP-KNEEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQL 302
           MDFA ++SIP K EE+E ARV+IWKYIFGFVEMAIVKCAIEL+I DTIESHGS MTLSQL
Sbjct: 1   MDFAPQKSIPKKQEEEEEARVEIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQL 60

Query: 303 SSALNCSSSLLYRILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLE 362
           S+ALNCS+SLLYRILRFLV RGIFK+EI E N+ SY QTPLSRLLASSNNNSMAPFLLLE
Sbjct: 61  STALNCSASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMAPFLLLE 120

Query: 363 SNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVP 422
           S+PVMLAPWH LSARIKG GETPFEAAHGKDVWSFA ADPIHN VINDAMSCTARVLTVP
Sbjct: 121 SSPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVP 180

Query: 423 AILEDCPEIFEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH- 482
           AILE+CP+IFEG+GSLVDVGGGNGT LSMIVKA PWIKGINFDLPHVISSSQ+YIGV+H 
Sbjct: 181 AILEECPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHV 240

Query: 483 -----------------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEE-- 542
                            WVLHDWDDETCIKILKNC+ AI +K GKVIIVEA+IEE+ E  
Sbjct: 241 GGNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKGAISEKRGKVIIVEALIEERSEEN 300

Query: 543 -NNMSDVGLMLDMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGFL 579
            N + DVGLMLDMVMMAHT NGKERT+KEW +VLHQAGFT++TITPIRAV S+IQ FL
Sbjct: 301 NNKLGDVGLMLDMVMMAHTKNGKERTSKEWGHVLHQAGFTQYTITPIRAVHSVIQAFL 358

BLAST of Lsi11G012330 vs. NCBI nr
Match: gi|659094140|ref|XP_008447906.1| (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 559.3 bits (1440), Expect = 7.9e-156
Identity = 288/359 (80.22%), Postives = 307/359 (85.52%), Query Frame = 1

Query: 243 MDFALKESIPKNEEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLS 302
           M+FA + SI K EE+E ARVQIWKYIFGFVEMAIVKCAIEL+I DTIESHGS MTLSQLS
Sbjct: 1   MNFAPQVSISKKEEEE-ARVQIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQLS 60

Query: 303 SALNCSSSLLYRILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLES 362
           +ALNCSSSLLYRILRFLV RGIFK+EITEEN+ SY  TPLSRLLASSNNNSMAP LLLES
Sbjct: 61  TALNCSSSLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMAPLLLLES 120

Query: 363 NPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPA 422
           +PVMLAPWH LSARIKG GETPFEAAHGKDVWSFA ADPIHN VINDAMSCTARV TVPA
Sbjct: 121 SPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVHTVPA 180

Query: 423 ILEDCPEIFEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH-- 482
           ILEDCP+IFEG+GSLVDVGGGNGT LSMIVKA PWIKGINFDLPHVISSSQ+YIGV+H  
Sbjct: 181 ILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHVG 240

Query: 483 ----------------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEE--- 542
                           WVLHDWDDETCIKILKNC++AI +K GKVIIVEAIIEE+ E   
Sbjct: 241 GNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKEAISEKRGKVIIVEAIIEERSEEEN 300

Query: 543 --NNMSDVGLMLDMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGFL 579
             NN+ DVGLMLDMVMMAHT NGKERT KEW  VL QAGFTR+TITPIRAV S+IQGFL
Sbjct: 301 NNNNLGDVGLMLDMVMMAHTENGKERTIKEWGNVLRQAGFTRYTITPIRAVHSVIQGFL 358

BLAST of Lsi11G012330 vs. NCBI nr
Match: gi|659094136|ref|XP_008447904.1| (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 449.9 bits (1156), Expect = 6.7e-123
Identity = 219/358 (61.17%), Postives = 278/358 (77.65%), Query Frame = 1

Query: 243 MDFALKESIPKNEEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLS 302
           M+   KES  + EED  A +Q+W+YIF F EMA +KCAI+LKIAD IES+GS +TLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCAIDLKIADIIESYGSPVTLSQLS 60

Query: 303 SALNCSSSLLYRILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLES 362
           S LNCSSSLLYRILRFL+HRGIFK E  +EN   Y+QTP+SRLLA +  NSMAP LLLE+
Sbjct: 61  STLNCSSSLLYRILRFLIHRGIFKRETIDENQIGYSQTPMSRLLAINVENSMAPLLLLET 120

Query: 363 NPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPA 422
           +PVMLAPW  LSA +K +  +PFE AHGKD+W++AEA+  HN + N+AM+C+A+V+ V A
Sbjct: 121 SPVMLAPWQHLSAHLKNSDTSPFEIAHGKDLWNYAEANHEHNLLFNEAMACSAKVI-VSA 180

Query: 423 ILEDCPEIFEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH-- 482
           I+E C ++F+GVG LVDVGGGNG++L+++VK CPW+KGINFDLPHV+ +S +Y  V+H  
Sbjct: 181 IIEGCGDVFDGVGCLVDVGGGNGSTLNILVKVCPWMKGINFDLPHVVCASPQYENVEHVA 240

Query: 483 ----------------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAII-----EEK 542
                           W+LHDW+DE CIKILK C++AIPK  GKVII+EAII      EK
Sbjct: 241 GNMFDFVPHADVAFLKWILHDWEDEECIKILKKCKEAIPKSGGKVIIIEAIIIEAEKGEK 300

Query: 543 EENNMSDVGLMLDMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGF 578
            +  +SDVGLM D+VMMAHTN G+ERTA+EWA+++HQAGFTRHTITP++A+QSLIQ F
Sbjct: 301 MKKKLSDVGLMFDLVMMAHTNKGRERTAEEWAFLIHQAGFTRHTITPLQAIQSLIQCF 357

BLAST of Lsi11G012330 vs. NCBI nr
Match: gi|449454255|ref|XP_004144871.1| (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 441.8 bits (1135), Expect = 1.8e-120
Identity = 221/346 (63.87%), Postives = 270/346 (78.03%), Query Frame = 1

Query: 255 EEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLSSALNCSSSLLYR 314
           EED + +VQIWKYIFGFVEMA++KCAIELKI D IESHG SMTLSQLSS+LNCS  LL+R
Sbjct: 16  EEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLSQLSSSLNCSPLLLHR 75

Query: 315 ILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLESNPVMLAPWHGLS 374
           ILRFLVHRGIFKEE T +    Y+ TP+SRLL ++  NSMAP  LLES+PVM+APWH LS
Sbjct: 76  ILRFLVHRGIFKEEKTTK---CYSHTPMSRLLTTTGPNSMAPLHLLESSPVMIAPWHNLS 135

Query: 375 ARIKGT----GETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPAILEDCPEI 434
           A +K +    G  PFE AHG D+W++A A+P H+++IN++M+C AR++ +PA+LE C EI
Sbjct: 136 ACVKASDHENGSQPFEMAHGMDLWTYAAANPSHSSLINESMACFARLVILPAVLERCGEI 195

Query: 435 FEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH---------- 494
           F+GVG LVDVGGGNGT LS++VKACPWIKGINFDLPHV+S S+EY G+QH          
Sbjct: 196 FDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGIQHVGGNMFDSVP 255

Query: 495 --------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIEEKEE-NNMSDVGLML 554
                    VLHDWDDE CIKILKNC++AIP+KTGKVIIVE +I+EKEE +  SD  LM+
Sbjct: 256 KADAAFIMEVLHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKEEISKYSDARLMM 315

Query: 555 DMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGF 578
           DM+MMAHT  GKERT +EWA VL +AGF+ +TITP   +QS+IQ F
Sbjct: 316 DMIMMAHTIKGKERTNEEWANVLQKAGFSYYTITPTSTIQSVIQAF 358

BLAST of Lsi11G012330 vs. NCBI nr
Match: gi|449470501|ref|XP_004152955.1| (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 441.8 bits (1135), Expect = 1.8e-120
Identity = 216/357 (60.50%), Postives = 274/357 (76.75%), Query Frame = 1

Query: 243 MDFALKESIPKNEEDENARVQIWKYIFGFVEMAIVKCAIELKIADTIESHGSSMTLSQLS 302
           M+   KES  + EED  A +Q+W+YIF F EMA +KC ++LKIAD IES+GS +TLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS 60

Query: 303 SALNCSSSLLYRILRFLVHRGIFKEEITEENLTSYAQTPLSRLLASSNNNSMAPFLLLES 362
           S LNCSSSLL+RILRFL+HRGIFK+E  +EN   Y+QTP+SRLLA++  NSMAP LLLE+
Sbjct: 61  STLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLET 120

Query: 363 NPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAEADPIHNTVINDAMSCTARVLTVPA 422
           +PVMLAPW  LS  +K +   PFE AHGKD+W +AEA+  HN + N+AM+C A+++ V A
Sbjct: 121 SPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMI-VSA 180

Query: 423 ILEDCPEIFEGVGSLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVISSSQEYIGVQH-- 482
           I+E C ++F+GV  LVDVGGGNG++LS++VKA PW+KGINFDLPHV+ +S +Y  V+H  
Sbjct: 181 IIEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVA 240

Query: 483 ----------------WVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAIIE----EKE 542
                           W+LHDWDDE CIKILK C+++IPK  GKVII+EAIIE    EK+
Sbjct: 241 GNMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKK 300

Query: 543 ENNMSDVGLMLDMVMMAHTNNGKERTAKEWAYVLHQAGFTRHTITPIRAVQSLIQGF 578
              +SDVGLM D+VMMAHTN GKERTA+EWA+++HQAGFT HTITPI+A+QSLIQ F
Sbjct: 301 NKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCF 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
7OMT_PAPSO2.2e-6539.89(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum GN=7OMT PE=1 SV=1[more]
4OMT2_PAPSO8.3e-6537.463'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferu... [more]
4OMT1_PAPSO4.6e-6338.443'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 1 OS=Papaver somniferu... [more]
6OMT_COPJA2.5e-6137.21(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica PE=1 SV=1[more]
4OMT_COPJA1.4e-5937.213'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0K2N4_CUCSA6.5e-15779.61Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017720 PE=3 SV=1[more]
A0A0A0K0Q2_CUCSA1.3e-12063.87Uncharacterized protein OS=Cucumis sativus GN=Csa_7G018720 PE=3 SV=1[more]
A0A0A0K629_CUCSA1.3e-12060.50Uncharacterized protein OS=Cucumis sativus GN=Csa_7G018730 PE=3 SV=1[more]
A0A061DXX0_THECC3.8e-11760.88RS-norcoclaurine 6-O-methyltransferase OS=Theobroma cacao GN=TCM_006540 PE=3 SV=... [more]
M5W6W6_PRUPE4.0e-11459.94Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007692mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G35160.18.0e-9049.58 O-methyltransferase family protein[more]
AT4G35150.11.1e-6742.24 O-methyltransferase family protein[more]
AT5G54160.13.4e-4032.44 O-methyltransferase 1[more]
AT1G77520.18.6e-3631.95 O-methyltransferase family protein[more]
AT1G77530.15.6e-3531.40 O-methyltransferase family protein[more]
Match NameE-valueIdentityDescription
gi|449454368|ref|XP_004144927.1|9.3e-15779.61PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis sativus][more]
gi|659094140|ref|XP_008447906.1|7.9e-15680.22PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo][more]
gi|659094136|ref|XP_008447904.1|6.7e-12361.17PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo][more]
gi|449454255|ref|XP_004144871.1|1.8e-12063.87PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis sativus][more]
gi|449470501|ref|XP_004152955.1|1.8e-12060.50PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008168methyltransferase activity
GO:0046983protein dimerization activity
GO:0008171O-methyltransferase activity
Vocabulary: INTERPRO
TermDefinition
IPR016461O-MeTrfase_COMT
IPR012967Plant_MeTrfase_dimerisation
IPR011991Winged helix-turn-helix DNA-binding domain
IPR001077O_MeTrfase_2
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008168 methyltransferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G012330.1Lsi11G012330.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001077O-methyltransferase, family 2PFAMPF00891Methyltransf_2coord: 480..561
score: 1.8E-23coord: 120..252
score: 1.2E-21coord: 362..476
score: 2.1
IPR011991Winged helix-turn-helix DNA-binding domainGENE3DG3DSA:1.10.10.10coord: 11..104
score: 4.5E-28coord: 253..346
score: 4.4
IPR011991Winged helix-turn-helix DNA-binding domainunknownSSF46785"Winged helix" DNA-binding domaincoord: 254..350
score: 1.36E-25coord: 12..108
score: 1.33
IPR012967Plant methyltransferase dimerisationPFAMPF08100Dimerisationcoord: 34..78
score: 1.3E-10coord: 276..320
score: 1.3
IPR016461O-methyltransferase COMT-typePROFILEPS51683SAM_OMT_IIcoord: 23..269
score: 33.873coord: 265..578
score: 51
NoneNo IPR availablePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 243..577
score: 1.9E
NoneNo IPR availablePANTHERPTHR11746:SF98O-METHYLTRANSFERASE FAMILY PROTEINcoord: 243..577
score: 1.9E

The following gene(s) are paralogous to this gene:

None