CSPI07G01310 (gene) Wild cucumber (PI 183967)

BLAST of CSPI07G01310 vs. Swiss-Prot
Match: 7OMT_PAPSO ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum GN=7OMT PE=1 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 9.9e-64
Identity = 145/364 (39.84%), Postives = 212/364 (58.24%), Query Frame = 1

Query: 13  KEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVLNCSPP--- 72
           +E  + + +I++++F F ++  +KCA+EL I ++I SHGRP +T+S++   L  + P   
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRP-VTISEIVDSLKTNTPSSS 64

Query: 73  ----LLFRIMRFLTHRGIFKEEITPE-NLIAYSQTPLSHMLI-----NVAPLLLLENSPE 132
                L RIMR L H+ +F  E+  E N + Y+ T  S  L+     N++PL+L E +P 
Sbjct: 65  PNIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPI 124

Query: 133 IVASWHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARV 192
           ++  W  L    Q   +         PFE AHG +IW     +P FN   N  + C+   
Sbjct: 125 LLKPWQYLGKCAQEKSS---------PFERAHGCEIWDLALADPKFNNFLNGAMQCSTTT 184

Query: 193 ITLPAILENCGDIFNGVE-SLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYD 252
           I    +LE   D F+G+  SLVDVGGG G+ ++ +VKA P ++GINFDLP+VV T+ ++ 
Sbjct: 185 IINEMLLE-YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFP 244

Query: 253 GVEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVI 312
           GV+HVGG+M   +P+ADA    WILH W DEDC  ILKNC  AI   K  GKVII+D V+
Sbjct: 245 GVKHVGGDMFVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAI-RKKKNGKVIIVDCVL 304

Query: 313 DENEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSI 363
              + N +   + L  D++MM  +  G+ERT  EW ++L+N AGF R  +   P A P I
Sbjct: 305 -RPDGNDLFDKMGLIFDVLMMAHTTAGKERTEAEW-KILLNNAGFPRYNVIRTP-AFPCI 353

BLAST of CSPI07G01310 vs. Swiss-Prot
Match: 6OMT_COPJA ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.6e-61
Identity = 144/360 (40.00%), Postives = 216/360 (60.00%), Query Frame = 1

Query: 12  KKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVLNCSP--- 71
           KK+   ++ +++ +++GFAE+ V+KCA++L +AN+I + G  +MTLS+LS+ L   P   
Sbjct: 4   KKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGT-SMTLSELSSRLPSQPVNE 63

Query: 72  PLLFRIMRFLTHRGIF-KEEITPENLIAYSQTPLSHMLIN-----VAPLLLLENSPEIVA 131
             L+R+MR+L H  +F K  I  E  + Y   P +  L+      +   +L     + +A
Sbjct: 64  DALYRVMRYLVHMKLFTKASIDGE--LRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMA 123

Query: 132 SWHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARVITL 191
            WH L   L               FE A G +IW Y A +P  N++FNE +A ++R+I +
Sbjct: 124 PWHYLKDGLSGESGT--------AFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLI-M 183

Query: 192 PAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEH 251
            A+++ CG+IFNG+ +LVDVGGG GT +  +  AFP +K   +DLP+V+  S  Y  V  
Sbjct: 184 SALVKECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHC 243

Query: 252 VGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENE 311
           V G+M  F+PKADA     ILH WDD++CI+ILK CKEA+     GGKVII+D V++   
Sbjct: 244 VAGDMFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVK--GGKVIIVDIVLNVQS 303

Query: 312 ENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQAF 363
           E+   T +RLTLD+ MM  +  G+ERT +EW + LI+ AG+    IT I  AV S+I+A+
Sbjct: 304 EHPY-TKMRLTLDLDMMLNT-GGKERTEEEWKK-LIHDAGYKGHKITQI-TAVQSVIEAY 345

BLAST of CSPI07G01310 vs. Swiss-Prot
Match: 6OMT_PAPSO ((RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum GN=6OMT PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.0e-60
Identity = 143/360 (39.72%), Postives = 222/360 (61.67%), Query Frame = 1

Query: 13  KEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVLNCSPPL-- 72
           K +++ + +I+K ++GFAE+ V+KCA++L+IA  + ++ +P M+LS+L++ L  + P+  
Sbjct: 6   KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKP-MSLSELASKLPVAQPVNE 65

Query: 73  --LFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLI-----NVAPLLLLENSPEIVAS 132
             LFRIMR+L H  +FK + T +    YS  P +  L+     ++   +L  N  + +A 
Sbjct: 66  DRLFRIMRYLVHMELFKIDATTQK---YSLAPPAKYLLRGWEKSMVDSILCINDKDFLAP 125

Query: 133 WHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARVITLP 192
           WH+L   L      N D+     FE A GK IW Y + NP  N++FN  +AC+ R++T  
Sbjct: 126 WHHLGDGL----TGNCDA-----FEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVT-S 185

Query: 193 AILENCGDIFN-GVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEH 252
           A+   C  IF+ G+ +LVDVGGG GT +  + KAFP +K   +DLP+V+  S +   +  
Sbjct: 186 ALANECKSIFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITK 245

Query: 253 VGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENE 312
           + G+M   +P ADA F   ILH W+D++CI+ILK CKEA+ +   GGKVII+D VID + 
Sbjct: 246 ISGDMFKSIPSADAIFMKCILHDWNDDECIQILKRCKEALPK---GGKVIIVDVVIDMDS 305

Query: 313 ENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQAF 363
            +  A  IRLTLD+ MM  +  G+ERT +EW + L + AGF+   +T I +AV S+I+A+
Sbjct: 306 THPYA-KIRLTLDLDMMLNT-GGKERTKEEW-KTLFDAAGFASHKVTQI-SAVQSVIEAY 344

BLAST of CSPI07G01310 vs. Swiss-Prot
Match: 4OMT2_PAPSO (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum GN=4'OMT2 PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.3e-60
Identity = 139/351 (39.60%), Postives = 207/351 (58.97%), Query Frame = 1

Query: 21  QIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVL---NCSPPLLFRIMRF 80
           Q++  ++GFA++ V++CA+E+ IA++I+++    +TL+QL+  L   N S   L+R++R+
Sbjct: 22  QLWNIIYGFADSLVLRCAVEIGIADIIKNNDG-AITLAQLAAKLPITNVSSDYLYRMVRY 81

Query: 81  LTHRGIFKEEITPENLI-AYSQTPLSHMLI-----NVAPLLLLENSPEIVASWHNLSARL 140
           L H  I ++E     +   YS  P+  +L+     ++ P++L     + + SWH +   L
Sbjct: 82  LVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEGL 141

Query: 141 QNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARVITLPAILENCGD 200
            N             FE   G DIW+Y   NP  +++FNEG+A   R++T   ++E+C D
Sbjct: 142 GNGSTT--------AFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLT-KTLIEDCRD 201

Query: 201 IFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHVGGNMLDFV 260
            F G++SLVD+GGGNGTT+  + +AFP +K   +DLP+VV  S     +E V G+M   V
Sbjct: 202 TFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSV 261

Query: 261 PKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENEENKMATDIR 320
           P A A     ILH W DE+C+ ILK CKEAI   K  GKVII+D  ++E E N   T  R
Sbjct: 262 PSAQAILLKLILHDWTDEECVNILKKCKEAI--PKETGKVIIVDVALEE-ESNHELTKTR 321

Query: 321 LTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQAF 363
           L LDI M+  +  GRERTAD+W  LL  +AGF    I PI  A+ S+I+AF
Sbjct: 322 LILDIDMLVNT-GGRERTADDWENLL-KRAGFRSHKIRPI-RAIQSVIEAF 356

BLAST of CSPI07G01310 vs. Swiss-Prot
Match: 4OMT_COPJA (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.1e-57
Identity = 131/356 (36.80%), Postives = 211/356 (59.27%), Query Frame = 1

Query: 17  EAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVL-----NCSPPLL 76
           +A+  ++K ++GFA++ V++CA+EL I ++I+++ +P M L+ L++ L     NC    L
Sbjct: 13  KAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQP-MALADLASKLPVSDVNCDN--L 72

Query: 77  FRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLI-----NVAPLLLLENSPEIVASWHN 136
           +RI+R+L    I + E + +    Y+  P++ +L      ++ P++L     + +  WH+
Sbjct: 73  YRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWHS 132

Query: 137 LSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARVITLPAIL 196
           +   L         SD    FE A G  IW Y   +P  +++FNEG+A   R++T  +++
Sbjct: 133 MKDGL---------SDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLT-SSLI 192

Query: 197 ENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHVGGN 256
               D+F G++SLVDVGGGNGTT+  +  AFP +K   FDLP+V+  S     +E +GG+
Sbjct: 193 SGSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGD 252

Query: 257 MLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENEENKM 316
           M   VP A A     ILH W+DED IKILK C+ A+   K GGKVII+D  +DE  ++++
Sbjct: 253 MFKSVPSAQAIILKLILHDWNDEDSIKILKQCRNAV--PKDGGKVIIVDVALDEESDHEL 312

Query: 317 ATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQAF 363
           ++  RL LDI M+  +  G+ERT + W + ++  AGFS C I  I AA+ S+I+ F
Sbjct: 313 SS-TRLILDIDMLVNT-GGKERTKEVW-EKIVKSAGFSGCKIRHI-AAIQSVIEVF 349

BLAST of CSPI07G01310 vs. TrEMBL
Match: A0A0A0K1F0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017710 PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 1.3e-206
Identity = 361/364 (99.18%), Postives = 361/364 (99.18%), Query Frame = 1

Query: 1   MEQTITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQL 60
           MEQTITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQL
Sbjct: 1   MEQTITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQL 60

Query: 61  STVLNCSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEI 120
           STVL CSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEI
Sbjct: 61  STVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEI 120

Query: 121 VASWHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARVI 180
           VASW NLSARLQNSYNNNDDSDLLVPFEVAHGKDIW YGARNPGFNRMFNEGLACNARVI
Sbjct: 121 VASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVI 180

Query: 181 TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV 240
           TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV
Sbjct: 181 TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV 240

Query: 241 EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE 300
           EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE
Sbjct: 241 EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE 300

Query: 301 NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQ 360
           NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQ
Sbjct: 301 NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQ 360

Query: 361 AFIS 365
           AFIS
Sbjct: 361 AFIS 364

BLAST of CSPI07G01310 vs. TrEMBL
Match: A0A0A0K2N4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017720 PE=3 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 4.7e-113
Identity = 212/366 (57.92%), Postives = 277/366 (75.68%), Query Frame = 1

Query: 7   KESIWKK--EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVL 66
           ++SI KK  EEEEA+++I+KY+FGF E A++KCAIEL+I + IESHG P MTLSQLST L
Sbjct: 6   QKSIPKKQEEEEEARVEIWKYIFGFVEMAIVKCAIELRIGDTIESHGSP-MTLSQLSTAL 65

Query: 67  NCSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLI-----NVAPLLLLENSPE 126
           NCS  LL+RI+RFL  RGIFK+EI   N+I+Y QTPLS +L      ++AP LLLE+SP 
Sbjct: 66  NCSASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMAPFLLLESSPV 125

Query: 127 IVASWHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARV 186
           ++A WH LSAR++ +           PFE AHGKD+W + A +P  N + N+ ++C ARV
Sbjct: 126 MLAPWHRLSARIKGNGE--------TPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARV 185

Query: 187 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDG 246
           +T+PAILE C  IF G+ SLVDVGGGNGT LS++VKAFPW+KGINFDLP+V+ +S++Y G
Sbjct: 186 LTVPAILEECPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIG 245

Query: 247 VEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVID 306
           VEHVGGNMLD +PKADAAF MW+LH WDDE CIKILKNCK AI E +  GKVII++++I+
Sbjct: 246 VEHVGGNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKGAISEKR--GKVIIVEALIE 305

Query: 307 E--NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPS 364
           E   E N    D+ L LD++MM  ++ G+ERT+ EW  +L ++AGF++ TITPI  AV S
Sbjct: 306 ERSEENNNKLGDVGLMLDMVMMAHTKNGKERTSKEWGHVL-HQAGFTQYTITPI-RAVHS 358

BLAST of CSPI07G01310 vs. TrEMBL
Match: A0A0A0K0Q2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G018720 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 3.4e-111
Identity = 219/370 (59.19%), Postives = 272/370 (73.51%), Query Frame = 1

Query: 1   MEQTITKESIWKKEEEE---AKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTL 60
           M+ T +K S   K EEE    K+QI+KY+FGF E AVIKCAIELKI +VIESHG  +MTL
Sbjct: 1   MDDTSSKASNKLKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHG-DSMTL 60

Query: 61  SQLSTVLNCSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLI-----NVAPLL 120
           SQLS+ LNCSP LL RI+RFL HRGIFKEE T +    YS TP+S +L      ++APL 
Sbjct: 61  SQLSSSLNCSPLLLHRILRFLVHRGIFKEEKTTK---CYSHTPMSRLLTTTGPNSMAPLH 120

Query: 121 LLENSPEIVASWHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEG 180
           LLE+SP ++A WHNLSA ++ S + N       PFE+AHG D+W Y A NP  + + NE 
Sbjct: 121 LLESSPVMIAPWHNLSACVKASDHENGSQ----PFEMAHGMDLWTYAAANPSHSSLINES 180

Query: 181 LACNARVITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVL 240
           +AC AR++ LPA+LE CG+IF+GV  LVDVGGGNGT LS+LVKA PW+KGINFDLP+VV 
Sbjct: 181 MACFARLVILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVS 240

Query: 241 TSEKYDGVEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVI 300
            S++Y+G++HVGGNM D VPKADAAF M +LH WDDE+CIKILKNCKEAI E    GKVI
Sbjct: 241 VSKEYEGIQHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKT--GKVI 300

Query: 301 IIDSVIDENEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIP 360
           I++ VIDE EE    +D RL +D++MM  + KG+ERT +EW  +L  KAGFS  TITP  
Sbjct: 301 IVEVVIDEKEEISKYSDARLMMDMIMMAHTIKGKERTNEEWANVL-QKAGFSYYTITP-T 358

Query: 361 AAVPSIIQAF 363
           + + S+IQAF
Sbjct: 361 STIQSVIQAF 358

BLAST of CSPI07G01310 vs. TrEMBL
Match: A0A0A0K629_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G018730 PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 6.0e-108
Identity = 207/365 (56.71%), Postives = 269/365 (73.70%), Query Frame = 1

Query: 7   KESIWKKEEE-EAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVLN 66
           KES  +KEE+ +A IQ+++Y+F F E A IKC ++LKIA++IES+G P +TLSQLS+ LN
Sbjct: 6   KESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSP-VTLSQLSSTLN 65

Query: 67  CSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLI-----NVAPLLLLENSPEI 126
           CS  LL RI+RFL HRGIFK+E   EN I YSQTP+S +L      ++APLLLLE SP +
Sbjct: 66  CSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLETSPVM 125

Query: 127 VASWHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARVI 186
           +A W +LS  L+NS          +PFE+AHGKD+WRY   N   N +FNE +AC A++I
Sbjct: 126 LAPWQHLSGHLKNSDT--------LPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMI 185

Query: 187 TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV 246
            + AI+E CGD+F+GV  LVDVGGGNG+TLS+LVKA+PWMKGINFDLP+VV  S +Y+ V
Sbjct: 186 -VSAIIEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENV 245

Query: 247 EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE 306
           EHV GNM DFVP AD AF  WILH WDDE+CIKILK CKE+I   K+GGKVIII+++I+ 
Sbjct: 246 EHVAGNMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIP--KSGGKVIIIEAIIEA 305

Query: 307 NE---ENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPS 363
            +   +NK  +D+ L  D++MM  + +G+ERTA EW   LI++AGF+  TITPI  A+ S
Sbjct: 306 EKGEKKNKKLSDVGLMFDLVMMAHTNRGKERTAQEWA-FLIHQAGFTTHTITPI-QAIQS 356

BLAST of CSPI07G01310 vs. TrEMBL
Match: A0A0D2R8U0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G038200 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 4.9e-102
Identity = 190/355 (53.52%), Postives = 266/355 (74.93%), Query Frame = 1

Query: 14  EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVLNCSPPLLFR 73
           E+E+A+++I+ YVFG+A+ AV+KCAIEL IA+ I+ HG P MTLSQL+T L C PP L+R
Sbjct: 13  EDEKAEVEIWNYVFGYAKIAVVKCAIELGIADAIDKHGSP-MTLSQLTTTLKCEPPRLYR 72

Query: 74  IMRFLTHRGIFKEEITPENLIAYSQTPLSHMLI-----NVAPLLLLENSPEIVASWHNLS 133
           I+RFL H  IFKEE   ++ I ++ TPLS  LI     ++A  +LLE+SP ++A WH+LS
Sbjct: 73  ILRFLVHYQIFKEEPVTQDSIGFALTPLSRRLIRHGERSMAAFILLESSPVMLAPWHSLS 132

Query: 134 ARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARVITLPAILEN 193
           AR+ +S N+        PFE AHGKD+W Y   NPG +++ +E +AC+ARV  + A++E 
Sbjct: 133 ARVLDSGNS--------PFETAHGKDVWSYAEENPGHSKLIDEAMACDARV-AVRALIEG 192

Query: 194 CGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHVGGNML 253
           C  +F+G++SLVDVGGGNGT LS+LVK FPWM GINFDLP+VV  + K DG+E+VGG+M 
Sbjct: 193 CPQVFDGIKSLVDVGGGNGTALSMLVKEFPWMHGINFDLPHVVAVAPKVDGIEYVGGDMF 252

Query: 254 DFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENEENKMAT 313
           + VP+ADAAFFMW+LH WDDE+CI+ILK C+EAI ++K  GKVII++SV++E+E +K+  
Sbjct: 253 ECVPRADAAFFMWVLHDWDDEECIQILKKCREAIPQDK--GKVIIVESVLEEDENDKLEF 312

Query: 314 DIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQAFI 364
            + L LD++MM  + KG+ERT  EW + ++ +AGF+R  + PI  AV SII+A+I
Sbjct: 313 -VGLMLDMVMMAHTNKGKERTLKEW-KYVLGEAGFTRINVKPI-HAVQSIIEAYI 352

BLAST of CSPI07G01310 vs. TAIR10
Match: AT4G35160.1 (AT4G35160.1 O-methyltransferase family protein)

HSP 1 Score: 304.7 bits (779), Expect = 7.8e-83
Identity = 166/367 (45.23%), Postives = 233/367 (63.49%), Query Frame = 1

Query: 14  EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESH-GRPTMTLSQLSTVLNCSPPLLF 73
           EE +A + I+KYVFGFA+ A  KCAI+LKI   IE+H     +TL++LS+ ++ SP  L 
Sbjct: 25  EEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHLR 84

Query: 74  RIMRFLTHRGIFKEEITPENLIA-YSQTPLSHMLI-------NVAPLLLLENSPEIVASW 133
           RIMRFL H+GIFKE  T + L   Y  TPLS  L+       ++AP +L E +PE++A W
Sbjct: 85  RIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAPW 144

Query: 134 HNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARVITLPA 193
             LS+ + +  N +       PF+  HGKD+W +   NP  + M NE +AC+AR + +P 
Sbjct: 145 LRLSSVVSSPVNGSTPP----PFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRV-VPR 204

Query: 194 ILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHVG 253
           +   C  +F+GV ++VDVGGG G T+ +LVK FPW+KG NFDLP+V+  +E  DGVE+V 
Sbjct: 205 VAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVE 264

Query: 254 GNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDEN--- 313
           G+M D +P  DA F  W+LH W D+DCIKILKNCKEA+  N   GKV+I++SVI EN   
Sbjct: 265 GDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNI--GKVLIVESVIGENKKT 324

Query: 314 ----EENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPS 365
               E ++    +RL LD++MM  +  G+ERT  EW   ++ +AGF+R  +  I   V S
Sbjct: 325 MIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEW-DFVLKEAGFARYEVRDID-DVQS 382

BLAST of CSPI07G01310 vs. TAIR10
Match: AT4G35150.1 (AT4G35150.1 O-methyltransferase family protein)

HSP 1 Score: 214.5 bits (545), Expect = 1.1e-55
Identity = 138/360 (38.33%), Postives = 198/360 (55.00%), Query Frame = 1

Query: 14  EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESH-GRPTMTLSQLSTVLNCSPPLLF 73
           EE +A + I++YVFGFA+ A  KCAI+LKI   IE+H     +TLS+LS+ ++ SP  L 
Sbjct: 11  EEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSHLR 70

Query: 74  RIMRFLTHRGIFKEEITPENL-IAYSQTPLSHMLINVAPLLLLENSPEIVASWHNLSARL 133
           RIMRFL H+G+FKE  T + L   Y+ TPLS  +     ++   +  ++   W      L
Sbjct: 71  RIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRM-----MITKLHGKDL---WAFAQDNL 130

Query: 134 QNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARVITLPAILENCGD 193
            +S   N+          A   D  R   R  G  +   +G+A                 
Sbjct: 131 CHSQLINE----------AMACDARRVVPRVAGACQGLFDGVAT---------------- 190

Query: 194 IFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHVGGNMLDFV 253
                  +VDVGGG G T+ +LVK FPW+KG NFDLP+V+  ++  DGVE+V G+M D +
Sbjct: 191 -------VVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSI 250

Query: 254 PKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENEENKMATD-- 313
           P +DA    W+LH W D+DCIKILKNCKEA+  N   GKV+I++ VI E +   +A +  
Sbjct: 251 PASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNI--GKVLIVECVIGEKKNTMIAEERD 310

Query: 314 -----IRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQAFIS 365
                +RL LD++MM  +  G+ERT  EW   ++ +AGF+R  +      V S+I A+ S
Sbjct: 311 DKLEHVRLQLDMVMMVHTSTGKERTLKEW-DFVLTEAGFARYEVRDFD-DVQSLIIAYRS 325

BLAST of CSPI07G01310 vs. TAIR10
Match: AT5G54160.1 (AT5G54160.1 O-methyltransferase 1)

HSP 1 Score: 184.9 bits (468), Expect = 9.0e-47
Identity = 110/322 (34.16%), Postives = 178/322 (55.28%), Query Frame = 1

Query: 35  IKCAIELKIANVIESHGRP---TMTLSQLSTVLNCSPPLLFRIMRFLTHRGIF---KEEI 94
           +K A+EL +  ++  +G P   T   S+L T    +P +L RI+R LT   +      ++
Sbjct: 36  LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKL 95

Query: 95  TPENLIA-YSQTPLSHML------INVAPLLLLENSPEIVASWHNLSARLQNSYNNNDDS 154
           + + +   Y   P+   L      +++A L L+     ++ SW++L   + +        
Sbjct: 96  SGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGG------ 155

Query: 155 DLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARVITLPAILENCGDIFNGVESLVD 214
              +PF  A+G   + Y   +P FN++FN G++ N   IT+  ILE     F G+ SLVD
Sbjct: 156 ---IPFNKAYGMSAFEYHGTDPRFNKVFNNGMS-NHSTITMKKILETYKG-FEGLTSLVD 215

Query: 215 VGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHVGGNMLDFVPKADAAFFMW 274
           VGGG G TL ++V  +P +KGINFDLP+V+  +  + G+EHVGG+M   VPK DA F  W
Sbjct: 216 VGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKW 275

Query: 275 ILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENEENKMATDIRLTLDIMMMTR 334
           I H W DE C+K LKNC E++ E+   GKVI+ + ++ E  ++ ++T   + +D +M+  
Sbjct: 276 ICHDWSDEHCVKFLKNCYESLPED---GKVILAECILPETPDSSLSTKQVVHVDCIMLAH 335

Query: 335 SRKGRERTADEWTQLLINKAGF 344
           +  G+ERT  E+ + L   +GF
Sbjct: 336 NPGGKERTEKEF-EALAKASGF 342

BLAST of CSPI07G01310 vs. TAIR10
Match: AT5G53810.1 (AT5G53810.1 O-methyltransferase family protein)

HSP 1 Score: 164.1 bits (414), Expect = 1.6e-40
Identity = 117/375 (31.20%), Postives = 184/375 (49.07%), Query Frame = 1

Query: 1   MEQTITKESIWKKE---EEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTL 60
           ++  +TK    K+E   EEEA++   +     A   V+K A+EL + + I + G   + L
Sbjct: 5   LQVPLTKPDRVKEEQEVEEEARLLARRLANAAASPMVLKAALELGVIDTITTVGGGDLWL 64

Query: 61  SQLSTVLNC--------SPPLLFRIMRFLTHRGIFK-EEITPEN------LIAYSQTPLS 120
           S     L          +P LL R++RFL    + K   +  EN         Y+  P+ 
Sbjct: 65  SPSEIALRLPTKPCNLEAPALLDRMLRFLVSHSVLKCRTVIEENGQTGKVERVYAAEPVC 124

Query: 121 HMLIN--------VAPLLLLENSPEIVASWHNLSARLQNSYNNNDDSDLLVPFEVAHGKD 180
             L+N         A L +L+ S   + +W +L   +    +          F  AHG  
Sbjct: 125 KYLLNKSDDVSGSFASLFMLDLSDVFIKTWTHLEDVILEGRD---------AFSSAHGMK 184

Query: 181 IWRYGARNPGFNRMFNEGLACNARVITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLV 240
           ++ Y   +  F ++FN  +  ++ ++T   +    G  F  V++LVDVGGG G TL ++ 
Sbjct: 185 LFEYIQADERFGKVFNRAMLESSTMVTEKVLKFYEG--FKDVKTLVDVGGGLGNTLGLIT 244

Query: 241 KAFPWMKGINFDLPNVVLTSEKYDGVEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKI 300
             +P + GINFDL  V+  +  Y GV HV G+M   +PK DA F  WILH W DE C+ I
Sbjct: 245 SKYPHLIGINFDLAPVLANAHSYPGVNHVAGDMFIKIPKGDAIFMKWILHDWTDEQCVAI 304

Query: 301 LKNCKEAIGENKAGGKVIIIDSVID-ENEENKMATDIRLTLDIMMMTRSRKGRERTADEW 349
           LKNC +++ EN   GK+II++ V   E +   + ++I   +D+ M+T+   G+ER   E+
Sbjct: 305 LKNCWKSLEEN---GKLIIVEMVTPVEAKSGDICSNIVFGMDMTMLTQCSGGKERDLYEF 364

BLAST of CSPI07G01310 vs. TAIR10
Match: AT1G77520.1 (AT1G77520.1 O-methyltransferase family protein)

HSP 1 Score: 156.4 bits (394), Expect = 3.4e-38
Identity = 115/370 (31.08%), Postives = 183/370 (49.46%), Query Frame = 1

Query: 5   ITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPT-MTLSQLSTV 64
           +TKE   + +E+   +Q    V   A   V+K A EL + + I + G  T ++  +++  
Sbjct: 17  LTKEE-QEVDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCEIACS 76

Query: 65  LNCSPP------LLFRIMRFLTHRGIFKEEI--TPEN------LIAYSQTPLSHMLI--- 124
           L   P       LL R++  L    I K  +  T EN         Y+  P+    +   
Sbjct: 77  LPTKPTNPEAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDS 136

Query: 125 ----NVAPLLLLENSPEIVASWHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARN 184
               ++ PL +L ++     +W NL   +    +          F  AHG  I+ Y   +
Sbjct: 137 DGTGSLVPLFMLLHTQVFFKTWTNLKDVILEGRD---------AFNSAHGMKIFEYINSD 196

Query: 185 PGFNRMFNEGLACNARVITLPAILENCGDIFNGVE---SLVDVGGGNGTTLSVLVKAFPW 244
             F  +FN  ++  + +I    +     D++ G E   +LVDVGGGNGT L ++   +P 
Sbjct: 197 QPFAELFNRAMSEPSTMIMKKVL-----DVYRGFEDVNTLVDVGGGNGTVLGLVTSKYPH 256

Query: 245 MKGINFDLPNVVLTSEKYDGVEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCK 304
           +KG+NFDL  V+  +  Y GVEHV G+M   VPK DA F  WILH W DEDCIKILKNC 
Sbjct: 257 IKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGDAVFMKWILHDWGDEDCIKILKNCW 316

Query: 305 EAIGENKAGGKVIIIDSVI-DENEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLI 349
           +++ E    GK+II++ V   E +   ++++    +D++M+T+   G+ER+  ++  L  
Sbjct: 317 KSLPEK---GKIIIVEFVTPKEPKGGDLSSNTVFAMDLLMLTQCSGGKERSLSQFENLAF 367

BLAST of CSPI07G01310 vs. NCBI nr
Match: gi|449454366|ref|XP_004144926.1| (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 726.9 bits (1875), Expect = 1.8e-206
Identity = 361/364 (99.18%), Postives = 361/364 (99.18%), Query Frame = 1

Query: 1   MEQTITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQL 60
           MEQTITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQL
Sbjct: 1   MEQTITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQL 60

Query: 61  STVLNCSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEI 120
           STVL CSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEI
Sbjct: 61  STVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEI 120

Query: 121 VASWHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARVI 180
           VASW NLSARLQNSYNNNDDSDLLVPFEVAHGKDIW YGARNPGFNRMFNEGLACNARVI
Sbjct: 121 VASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVI 180

Query: 181 TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV 240
           TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV
Sbjct: 181 TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV 240

Query: 241 EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE 300
           EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE
Sbjct: 241 EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE 300

Query: 301 NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQ 360
           NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQ
Sbjct: 301 NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQ 360

Query: 361 AFIS 365
           AFIS
Sbjct: 361 AFIS 364

BLAST of CSPI07G01310 vs. NCBI nr
Match: gi|659094531|ref|XP_008448112.1| (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 501.9 bits (1291), Expect = 9.4e-139
Identity = 247/261 (94.64%), Postives = 254/261 (97.32%), Query Frame = 1

Query: 1   MEQTITKESIWKK-EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60
           ME+TITKES WKK EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ
Sbjct: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60

Query: 61  LSTVLNCSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPE 120
           LST LNCSPPLLFRIMRFLTHRGIF+EE TPENLIAYSQTPLSHMLINVAPLLLLENSPE
Sbjct: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120

Query: 121 IVASWHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARV 180
           IVASWHNLSA+L+NSYNNN++SDL+VPFEVAHGKDIW YGARNPGFNRMFNEGL CNARV
Sbjct: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180

Query: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDG 240
           ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVV TSEKYDG
Sbjct: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240

Query: 241 VEHVGGNMLDFVPKADAAFFM 261
           VEHVGGNMLDFVPKADAAFFM
Sbjct: 241 VEHVGGNMLDFVPKADAAFFM 261

BLAST of CSPI07G01310 vs. NCBI nr
Match: gi|659094140|ref|XP_008447906.1| (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 424.9 bits (1091), Expect = 1.5e-115
Identity = 216/364 (59.34%), Postives = 274/364 (75.27%), Query Frame = 1

Query: 9   SIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVLNCSP 68
           SI KKEEEEA++QI+KY+FGF E A++KCAIEL+I + IESHG P MTLSQLST LNCS 
Sbjct: 8   SISKKEEEEARVQIWKYIFGFVEMAIVKCAIELRIGDTIESHGSP-MTLSQLSTALNCSS 67

Query: 69  PLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLI-----NVAPLLLLENSPEIVAS 128
            LL+RI+RFL  RGIFK+EIT EN+I+Y  TPLS +L      ++APLLLLE+SP ++A 
Sbjct: 68  SLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMAPLLLLESSPVMLAP 127

Query: 129 WHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARVITLP 188
           WH LSAR++ +           PFE AHGKD+W + A +P  N + N+ ++C ARV T+P
Sbjct: 128 WHRLSARIKGNGE--------TPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVHTVP 187

Query: 189 AILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHV 248
           AILE+C  IF G+ SLVDVGGGNGT LS++VKAFPW+KGINFDLP+V+ +S++Y GVEHV
Sbjct: 188 AILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHV 247

Query: 249 GGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENEE 308
           GGNMLD +PKADAAF MW+LH WDDE CIKILKNCKEAI E +  GKVII++++I+E  E
Sbjct: 248 GGNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKEAISEKR--GKVIIVEAIIEERSE 307

Query: 309 ----NKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSII 364
               N    D+ L LD++MM  +  G+ERT  EW  +L  +AGF+R TITPI  AV S+I
Sbjct: 308 EENNNNNLGDVGLMLDMVMMAHTENGKERTIKEWGNVL-RQAGFTRYTITPI-RAVHSVI 358

BLAST of CSPI07G01310 vs. NCBI nr
Match: gi|449454368|ref|XP_004144927.1| (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 416.0 bits (1068), Expect = 6.8e-113
Identity = 212/366 (57.92%), Postives = 277/366 (75.68%), Query Frame = 1

Query: 7   KESIWKK--EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVL 66
           ++SI KK  EEEEA+++I+KY+FGF E A++KCAIEL+I + IESHG P MTLSQLST L
Sbjct: 6   QKSIPKKQEEEEEARVEIWKYIFGFVEMAIVKCAIELRIGDTIESHGSP-MTLSQLSTAL 65

Query: 67  NCSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLI-----NVAPLLLLENSPE 126
           NCS  LL+RI+RFL  RGIFK+EI   N+I+Y QTPLS +L      ++AP LLLE+SP 
Sbjct: 66  NCSASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMAPFLLLESSPV 125

Query: 127 IVASWHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEGLACNARV 186
           ++A WH LSAR++ +           PFE AHGKD+W + A +P  N + N+ ++C ARV
Sbjct: 126 MLAPWHRLSARIKGNGE--------TPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARV 185

Query: 187 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDG 246
           +T+PAILE C  IF G+ SLVDVGGGNGT LS++VKAFPW+KGINFDLP+V+ +S++Y G
Sbjct: 186 LTVPAILEECPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIG 245

Query: 247 VEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVID 306
           VEHVGGNMLD +PKADAAF MW+LH WDDE CIKILKNCK AI E +  GKVII++++I+
Sbjct: 246 VEHVGGNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKGAISEKR--GKVIIVEALIE 305

Query: 307 E--NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPS 364
           E   E N    D+ L LD++MM  ++ G+ERT+ EW  +L ++AGF++ TITPI  AV S
Sbjct: 306 ERSEENNNKLGDVGLMLDMVMMAHTKNGKERTSKEWGHVL-HQAGFTQYTITPI-RAVHS 358

BLAST of CSPI07G01310 vs. NCBI nr
Match: gi|449454255|ref|XP_004144871.1| (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 409.8 bits (1052), Expect = 4.9e-111
Identity = 219/370 (59.19%), Postives = 272/370 (73.51%), Query Frame = 1

Query: 1   MEQTITKESIWKKEEEE---AKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTL 60
           M+ T +K S   K EEE    K+QI+KY+FGF E AVIKCAIELKI +VIESHG  +MTL
Sbjct: 1   MDDTSSKASNKLKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHG-DSMTL 60

Query: 61  SQLSTVLNCSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLI-----NVAPLL 120
           SQLS+ LNCSP LL RI+RFL HRGIFKEE T +    YS TP+S +L      ++APL 
Sbjct: 61  SQLSSSLNCSPLLLHRILRFLVHRGIFKEEKTTK---CYSHTPMSRLLTTTGPNSMAPLH 120

Query: 121 LLENSPEIVASWHNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWRYGARNPGFNRMFNEG 180
           LLE+SP ++A WHNLSA ++ S + N       PFE+AHG D+W Y A NP  + + NE 
Sbjct: 121 LLESSPVMIAPWHNLSACVKASDHENGSQ----PFEMAHGMDLWTYAAANPSHSSLINES 180

Query: 181 LACNARVITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVL 240
           +AC AR++ LPA+LE CG+IF+GV  LVDVGGGNGT LS+LVKA PW+KGINFDLP+VV 
Sbjct: 181 MACFARLVILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVS 240

Query: 241 TSEKYDGVEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVI 300
            S++Y+G++HVGGNM D VPKADAAF M +LH WDDE+CIKILKNCKEAI E    GKVI
Sbjct: 241 VSKEYEGIQHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKT--GKVI 300

Query: 301 IIDSVIDENEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIP 360
           I++ VIDE EE    +D RL +D++MM  + KG+ERT +EW  +L  KAGFS  TITP  
Sbjct: 301 IVEVVIDEKEEISKYSDARLMMDMIMMAHTIKGKERTNEEWANVL-QKAGFSYYTITP-T 358

Query: 361 AAVPSIIQAF 363
           + + S+IQAF
Sbjct: 361 STIQSVIQAF 358