CsaV3_7G001370 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_7G001370 vs. NCBI nr
Match: XP_004144926.1 (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis sativus] >KGN43288.1 hypothetical protein Csa_7G017710 [Cucumis sativus])

HSP 1 Score: 732.3 bits (1889), Expect = 8.3e-208
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MEQTITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQL 60
           MEQTITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQL
Sbjct: 1   MEQTITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQL 60

Query: 61  STVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEI 120
           STVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEI
Sbjct: 61  STVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEI 120

Query: 121 VASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVI 180
           VASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVI
Sbjct: 121 VASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVI 180

Query: 181 TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV 240
           TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV
Sbjct: 181 TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV 240

Query: 241 EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE 300
           EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE
Sbjct: 241 EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE 300

Query: 301 NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQ 360
           NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQ
Sbjct: 301 NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQ 360

Query: 361 AFIS 365
           AFIS
Sbjct: 361 AFIS 364

BLAST of CsaV3_7G001370 vs. NCBI nr
Match: XP_023554129.1 (xanthohumol 4'-O-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 498.0 bits (1281), Expect = 2.6e-137
Identity = 258/361 (71.47%), Postives = 289/361 (80.06%), Query Frame = 0

Query: 6   TKESIWKK--EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTV 65
           +KES WKK              VFGFAEAAVIKCA+ELKIA+ IESHGRP MTLSQLS+ 
Sbjct: 5   SKESSWKKXXXXXXXXXXXXXXVFGFAEAAVIKCAVELKIADAIESHGRP-MTLSQLSSA 64

Query: 66  LICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEIVAS 125
           L CSPPLLFRI+RFL HRGIFK++ T E  IAYSQTPLS ML  +A LLLLENSP +VA 
Sbjct: 65  LNCSPPLLFRILRFLVHRGIFKQQTTAEGPIAYSQTPLSRMLTTMASLLLLENSPTMVAP 124

Query: 126 WQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVITLP 185
           W NLSA+++     + +      FE AHGKDIWSY A NP FN +FNEGL CNARV+TLP
Sbjct: 125 WHNLSAKVKADAAGHSNL-----FEAAHGKDIWSYAAANPAFNTLFNEGLDCNARVVTLP 184

Query: 186 AILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHV 245
           AIL +C D+FNGV SLVDVGGGNGTTLSVLVKA PWMKGINFDLPNVV TS++Y+GV+HV
Sbjct: 185 AILGSCRDMFNGVGSLVDVGGGNGTTLSVLVKACPWMKGINFDLPNVVSTSQEYEGVQHV 244

Query: 246 GGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENEE 305
           GG+M D+VPKADAAFFMWILHAWDDE+CIKILKNCKEAI EN   GKV+I+D+VIDE EE
Sbjct: 245 GGSMFDYVPKADAAFFMWILHAWDDEECIKILKNCKEAIPENT--GKVMIVDTVIDEKEE 304

Query: 306 NKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQAFI 365
           NK+  DIRLTLDIM+MTR RKGRERT +EW  LL  KAGFSRC ITPI   VPSIIQAFI
Sbjct: 305 NKLLLDIRLTLDIMLMTRFRKGRERTGEEWANLL-KKAGFSRCIITPIVGVVPSIIQAFI 356

BLAST of CsaV3_7G001370 vs. NCBI nr
Match: XP_022969149.1 (xanthohumol 4'-O-methyltransferase-like [Cucurbita maxima])

HSP 1 Score: 491.1 bits (1263), Expect = 3.2e-135
Identity = 254/362 (70.17%), Postives = 287/362 (79.28%), Query Frame = 0

Query: 6   TKESIWK---KEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLST 65
           +KES WK               Y+FGFAEAAVIKCAIEL+IA+ IESHG P MTLSQLS+
Sbjct: 5   SKESSWKXXXXXXXXXXXXXXXYIFGFAEAAVIKCAIELQIADAIESHGSP-MTLSQLSS 64

Query: 66  VLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEIVA 125
            L CSP LLFRI+RFL HRGIFK++ T E+ IAYS TPLS ML  +APLLLLENSP +VA
Sbjct: 65  ALNCSPSLLFRILRFLVHRGIFKQQTTTESPIAYSHTPLSRMLTTMAPLLLLENSPTMVA 124

Query: 126 SWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVITL 185
            W NLSA+++     + +      FE AHGKDIWSY A NP FN +FNEGL CNARV+TL
Sbjct: 125 PWHNLSAKVKADAAGHSNL-----FEAAHGKDIWSYAAANPAFNTLFNEGLGCNARVVTL 184

Query: 186 PAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEH 245
           PAIL +C D+FNGV SLVDVGGGNGTTLS+LVKA PWMKGINFDLPNVV TS++Y+GV+H
Sbjct: 185 PAILGSCRDMFNGVGSLVDVGGGNGTTLSLLVKACPWMKGINFDLPNVVNTSQEYEGVQH 244

Query: 246 VGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENE 305
           VGG+M D+VPKADAAFFMWILHAWDDE+CIKILKNCKEAI EN   GKVII+D+V+DE E
Sbjct: 245 VGGSMFDYVPKADAAFFMWILHAWDDEECIKILKNCKEAIPENT--GKVIIVDTVMDEKE 304

Query: 306 ENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQAF 365
           ENKM  DIRLTLDIM+MTR RKGRERT +EW  LL  KAGFSRC ITPI   VPSIIQAF
Sbjct: 305 ENKMLLDIRLTLDIMLMTRFRKGRERTGEEWANLL-KKAGFSRCIITPIVGVVPSIIQAF 357

BLAST of CsaV3_7G001370 vs. NCBI nr
Match: XP_022952487.1 (xanthohumol 4'-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 482.3 bits (1240), Expect = 1.5e-132
Identity = 246/334 (73.65%), Postives = 275/334 (82.34%), Query Frame = 0

Query: 31  EAAVIKCAIELKIANVIESHGRPTMTLSQLSTVLICSPPLLFRIMRFLTHRGIFKEEITP 90
           EAAVIKCAIELKI + I SHG P MTLSQLS+ L CSPPLLFRI+RFL HRGIFK++ T 
Sbjct: 33  EAAVIKCAIELKIPDAIGSHGSP-MTLSQLSSALNCSPPLLFRILRFLVHRGIFKQQTTA 92

Query: 91  ENLIAYSQTPLSHMLINVAPLLLLENSPEIVASWQNLSARLQNSYNNNDDSDLLVPFEVA 150
           E  IAYSQTPLS ML  +APLLLLENSP +VA W NLSA+++     + +      FE A
Sbjct: 93  EGPIAYSQTPLSRMLTTMAPLLLLENSPTMVAPWHNLSAKVKADAAGHSNL-----FEAA 152

Query: 151 HGKDIWSYGARNPGFNRMFNEGLACNARVITLPAILENCGDIFNGVESLVDVGGGNGTTL 210
           HGKDIWSY A NP FN +FNEGL CNARV+TLPAIL +C D+FNGV SLVDVGGGNGTTL
Sbjct: 153 HGKDIWSYAAANPAFNVLFNEGLGCNARVVTLPAILGSCRDMFNGVGSLVDVGGGNGTTL 212

Query: 211 SVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHVGGNMLDFVPKADAAFFMWILHAWDDED 270
           SVLVKA PWMKGINFDLPNVV TS++Y+GV+HVGG+M D+VPKADAAFFMWILHAWDDE+
Sbjct: 213 SVLVKACPWMKGINFDLPNVVNTSQEYEGVQHVGGSMFDYVPKADAAFFMWILHAWDDEE 272

Query: 271 CIKILKNCKEAIGENKAGGKVIIIDSVIDENEENKMATDIRLTLDIMMMTRSRKGRERTA 330
           CIKILKNCKEAI EN   GKV+I+D+VIDE EENK+  DIRLTLDIM+MTR RKGRERT 
Sbjct: 273 CIKILKNCKEAIPENT--GKVMIVDTVIDEKEENKLLMDIRLTLDIMLMTRFRKGRERTG 332

Query: 331 DEWTQLLINKAGFSRCTITPIPAAVPSIIQAFIS 365
           +EW  LL  KAGFSRC ITPI   VPSIIQAFIS
Sbjct: 333 EEWANLL-KKAGFSRCIITPIVGVVPSIIQAFIS 357

BLAST of CsaV3_7G001370 vs. NCBI nr
Match: XP_016900458.1 (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 463.8 bits (1192), Expect = 5.5e-127
Identity = 232/261 (88.89%), Postives = 239/261 (91.57%), Query Frame = 0

Query: 1   MEQTITKESIWKK-EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60
           ME+TITKES WKK               GFAEAAVIKCAIELKIANVIESHGRPTMTLSQ
Sbjct: 1   MEETITKESKWKKXXXXXXXXXXXXXXXGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60

Query: 61  LSTVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPE 120
           LST L CSPPLLFRIMRFLTHRGIF+EE TPENLIAYSQTPLSHMLINVAPLLLLENSPE
Sbjct: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120

Query: 121 IVASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARV 180
           IVASW NLSA+L+NSYNNN++SDL+VPFEVAHGKDIWSYGARNPGFNRMFNEGL CNARV
Sbjct: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180

Query: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDG 240
           ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVV TSEKYDG
Sbjct: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240

Query: 241 VEHVGGNMLDFVPKADAAFFM 261
           VEHVGGNMLDFVPKADAAFFM
Sbjct: 241 VEHVGGNMLDFVPKADAAFFM 261

BLAST of CsaV3_7G001370 vs. TAIR10
Match: AT4G35160.1 (O-methyltransferase family protein)

HSP 1 Score: 305.1 bits (780), Expect = 5.9e-83
Identity = 167/367 (45.50%), Postives = 233/367 (63.49%), Query Frame = 0

Query: 14  EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESH-GRPTMTLSQLSTVLICSPPLLF 73
           EE +A + I+KYVFGFA+ A  KCAI+LKI   IE+H     +TL++LS+ +  SP  L 
Sbjct: 25  EEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHLR 84

Query: 74  RIMRFLTHRGIFKEEITPENL-IAYSQTPLSHMLI-------NVAPLLLLENSPEIVASW 133
           RIMRFL H+GIFKE  T + L   Y  TPLS  L+       ++AP +L E +PE++A W
Sbjct: 85  RIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAPW 144

Query: 134 QNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVITLPA 193
             LS+ + +  N +       PF+  HGKD+WS+   NP  + M NE +AC+AR + +P 
Sbjct: 145 LRLSSVVSSPVNGSTPP----PFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRV-VPR 204

Query: 194 ILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHVG 253
           +   C  +F+GV ++VDVGGG G T+ +LVK FPW+KG NFDLP+V+  +E  DGVE+V 
Sbjct: 205 VAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVE 264

Query: 254 GNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDEN--- 313
           G+M D +P  DA F  W+LH W D+DCIKILKNCKEA+  N   GKV+I++SVI EN   
Sbjct: 265 GDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNI--GKVLIVESVIGENKKT 324

Query: 314 ----EENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPS 365
               E ++    +RL LD++MM  +  G+ERT  EW   ++ +AGF+R  +  I   V S
Sbjct: 325 MIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEW-DFVLKEAGFARYEVRDID-DVQS 382

BLAST of CsaV3_7G001370 vs. TAIR10
Match: AT4G35150.1 (O-methyltransferase family protein)

HSP 1 Score: 255.4 bits (651), Expect = 5.4e-68
Identity = 144/360 (40.00%), Postives = 208/360 (57.78%), Query Frame = 0

Query: 14  EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESH-GRPTMTLSQLSTVLICSPPLLF 73
           EE +A + I++YVFGFA+ A  KCAI+LKI   IE+H     +TLS+LS+ +  SP  L 
Sbjct: 11  EEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSHLR 70

Query: 74  RIMRFLTHRGIFKEEITPENL-IAYSQTPLSHMLINVAPLLLLENSPEIVASWQNLSARL 133
           RIMRFL H+G+FKE  T + L   Y+ TPLS  ++                         
Sbjct: 71  RIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM------------------------- 130

Query: 134 QNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVITLPAILENCGD 193
                              HGKD+W++   N   +++ NE +AC+AR + +P +   C  
Sbjct: 131 ---------------ITKLHGKDLWAFAQDNLCHSQLINEAMACDARRV-VPRVAGACQG 190

Query: 194 IFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHVGGNMLDFV 253
           +F+GV ++VDVGGG G T+ +LVK FPW+KG NFDLP+V+  ++  DGVE+V G+M D +
Sbjct: 191 LFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSI 250

Query: 254 PKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENEENKMATD-- 313
           P +DA    W+LH W D+DCIKILKNCKEA+  N   GKV+I++ VI E +   +A +  
Sbjct: 251 PASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNI--GKVLIVECVIGEKKNTMIAEERD 310

Query: 314 -----IRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQAFIS 365
                +RL LD++MM  +  G+ERT  EW   ++ +AGF+R  +      V S+I A+ S
Sbjct: 311 DKLEHVRLQLDMVMMVHTSTGKERTLKEW-DFVLTEAGFARYEVRDFD-DVQSLIIAYRS 325

BLAST of CsaV3_7G001370 vs. TAIR10
Match: AT5G54160.1 (O-methyltransferase 1)

HSP 1 Score: 183.0 bits (463), Expect = 3.4e-46
Identity = 111/322 (34.47%), Postives = 177/322 (54.97%), Query Frame = 0

Query: 35  IKCAIELKIANVIESHG---RPTMTLSQLSTVLICSPPLLFRIMRFLTHRGIF---KEEI 94
           +K A+EL +  ++  +G    PT   S+L T    +P +L RI+R LT   +      ++
Sbjct: 36  LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKL 95

Query: 95  TPENL-IAYSQTPLSHML------INVAPLLLLENSPEIVASWQNLSARLQNSYNNNDDS 154
           + + +   Y   P+   L      +++A L L+     ++ SW +L   +          
Sbjct: 96  SGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAI---------L 155

Query: 155 DLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVITLPAILENCGDIFNGVESLVD 214
           D  +PF  A+G   + Y   +P FN++FN G++ N   IT+  ILE     F G+ SLVD
Sbjct: 156 DGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMS-NHSTITMKKILETYKG-FEGLTSLVD 215

Query: 215 VGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHVGGNMLDFVPKADAAFFMW 274
           VGGG G TL ++V  +P +KGINFDLP+V+  +  + G+EHVGG+M   VPK DA F  W
Sbjct: 216 VGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKW 275

Query: 275 ILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENEENKMATDIRLTLDIMMMTR 334
           I H W DE C+K LKNC E++ E+   GKVI+ + ++ E  ++ ++T   + +D +M+  
Sbjct: 276 ICHDWSDEHCVKFLKNCYESLPED---GKVILAECILPETPDSSLSTKQVVHVDCIMLAH 335

Query: 335 SRKGRERTADEWTQLLINKAGF 344
           +  G+ERT  E+ + L   +GF
Sbjct: 336 NPGGKERTEKEF-EALAKASGF 342

BLAST of CsaV3_7G001370 vs. TAIR10
Match: AT5G53810.1 (O-methyltransferase family protein)

HSP 1 Score: 162.9 bits (411), Expect = 3.7e-40
Identity = 117/375 (31.20%), Postives = 185/375 (49.33%), Query Frame = 0

Query: 1   MEQTITKESIWKKE---EEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTL 60
           ++  +TK    K+E   EEEA++   +     A   V+K A+EL + + I + G   + L
Sbjct: 5   LQVPLTKPDRVKEEQEVEEEARLLARRLANAAASPMVLKAALELGVIDTITTVGGGDLWL 64

Query: 61  SQLSTVL--------ICSPPLLFRIMRFLTHRGIFK-EEITPEN------LIAYSQTPLS 120
           S     L        + +P LL R++RFL    + K   +  EN         Y+  P+ 
Sbjct: 65  SPSEIALRLPTKPCNLEAPALLDRMLRFLVSHSVLKCRTVIEENGQTGKVERVYAAEPVC 124

Query: 121 HMLIN--------VAPLLLLENSPEIVASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKD 180
             L+N         A L +L+ S   + +W +L   +    +          F  AHG  
Sbjct: 125 KYLLNKSDDVSGSFASLFMLDLSDVFIKTWTHLEDVILEGRD---------AFSSAHGMK 184

Query: 181 IWSYGARNPGFNRMFNEGLACNARVITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLV 240
           ++ Y   +  F ++FN  +  ++ ++T   +    G  F  V++LVDVGGG G TL ++ 
Sbjct: 185 LFEYIQADERFGKVFNRAMLESSTMVTEKVLKFYEG--FKDVKTLVDVGGGLGNTLGLIT 244

Query: 241 KAFPWMKGINFDLPNVVLTSEKYDGVEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKI 300
             +P + GINFDL  V+  +  Y GV HV G+M   +PK DA F  WILH W DE C+ I
Sbjct: 245 SKYPHLIGINFDLAPVLANAHSYPGVNHVAGDMFIKIPKGDAIFMKWILHDWTDEQCVAI 304

Query: 301 LKNCKEAIGENKAGGKVIIIDSVID-ENEENKMATDIRLTLDIMMMTRSRKGRERTADEW 349
           LKNC +++ EN   GK+II++ V   E +   + ++I   +D+ M+T+   G+ER   E+
Sbjct: 305 LKNCWKSLEEN---GKLIIVEMVTPVEAKSGDICSNIVFGMDMTMLTQCSGGKERDLYEF 364

BLAST of CsaV3_7G001370 vs. TAIR10
Match: AT1G77520.1 (O-methyltransferase family protein)

HSP 1 Score: 155.6 bits (392), Expect = 5.8e-38
Identity = 115/370 (31.08%), Postives = 185/370 (50.00%), Query Frame = 0

Query: 5   ITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPT-MTLSQLSTV 64
           +TKE   + +E+   +Q    V   A   V+K A EL + + I + G  T ++  +++  
Sbjct: 17  LTKEE-QEVDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCEIACS 76

Query: 65  LIC------SPPLLFRIMRFLTHRGIFKEEI--TPEN------LIAYSQTPLSHMLI--- 124
           L        +P LL R++  L    I K  +  T EN         Y+  P+    +   
Sbjct: 77  LPTKPTNPEAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDS 136

Query: 125 ----NVAPLLLLENSPEIVASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARN 184
               ++ PL +L ++     +W NL   +    +          F  AHG  I+ Y   +
Sbjct: 137 DGTGSLVPLFMLLHTQVFFKTWTNLKDVILEGRD---------AFNSAHGMKIFEYINSD 196

Query: 185 PGFNRMFNEGLACNARVITLPAILENCGDIFNG---VESLVDVGGGNGTTLSVLVKAFPW 244
             F  +FN  ++  + +     I++   D++ G   V +LVDVGGGNGT L ++   +P 
Sbjct: 197 QPFAELFNRAMSEPSTM-----IMKKVLDVYRGFEDVNTLVDVGGGNGTVLGLVTSKYPH 256

Query: 245 MKGINFDLPNVVLTSEKYDGVEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCK 304
           +KG+NFDL  V+  +  Y GVEHV G+M   VPK DA F  WILH W DEDCIKILKNC 
Sbjct: 257 IKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGDAVFMKWILHDWGDEDCIKILKNCW 316

Query: 305 EAIGENKAGGKVIIIDSVI-DENEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLI 349
           +++ E    GK+II++ V   E +   ++++    +D++M+T+   G+ER+  ++  L  
Sbjct: 317 KSLPEK---GKIIIVEFVTPKEPKGGDLSSNTVFAMDLLMLTQCSGGKERSLSQFENLAF 367

BLAST of CsaV3_7G001370 vs. Swiss-Prot
Match: sp|Q9T003|ASMT_ARATH (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 1.1e-81
Identity = 167/367 (45.50%), Postives = 233/367 (63.49%), Query Frame = 0

Query: 14  EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESH-GRPTMTLSQLSTVLICSPPLLF 73
           EE +A + I+KYVFGFA+ A  KCAI+LKI   IE+H     +TL++LS+ +  SP  L 
Sbjct: 25  EEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHLR 84

Query: 74  RIMRFLTHRGIFKEEITPENL-IAYSQTPLSHMLI-------NVAPLLLLENSPEIVASW 133
           RIMRFL H+GIFKE  T + L   Y  TPLS  L+       ++AP +L E +PE++A W
Sbjct: 85  RIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAPW 144

Query: 134 QNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVITLPA 193
             LS+ + +  N +       PF+  HGKD+WS+   NP  + M NE +AC+AR + +P 
Sbjct: 145 LRLSSVVSSPVNGSTPP----PFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRV-VPR 204

Query: 194 ILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHVG 253
           +   C  +F+GV ++VDVGGG G T+ +LVK FPW+KG NFDLP+V+  +E  DGVE+V 
Sbjct: 205 VAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVE 264

Query: 254 GNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDEN--- 313
           G+M D +P  DA F  W+LH W D+DCIKILKNCKEA+  N   GKV+I++SVI EN   
Sbjct: 265 GDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNI--GKVLIVESVIGENKKT 324

Query: 314 ----EENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPS 365
               E ++    +RL LD++MM  +  G+ERT  EW   ++ +AGF+R  +  I   V S
Sbjct: 325 MIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEW-DFVLKEAGFARYEVRDID-DVQS 382

BLAST of CsaV3_7G001370 vs. Swiss-Prot
Match: sp|Q6WUC2|7OMT_PAPSO ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 5.9e-64
Identity = 146/364 (40.11%), Postives = 213/364 (58.52%), Query Frame = 0

Query: 13  KEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVLICSPP--- 72
           +E  + + +I++++F F ++  +KCA+EL I ++I SHGRP +T+S++   L  + P   
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRP-VTISEIVDSLKTNTPSSS 64

Query: 73  ----LLFRIMRFLTHRGIFKEEITPE-NLIAYSQTPLSHMLI-----NVAPLLLLENSPE 132
                L RIMR L H+ +F  E+  E N + Y+ T  S  L+     N++PL+L E +P 
Sbjct: 65  PNIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPI 124

Query: 133 IVASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARV 192
           ++  WQ L    Q   +         PFE AHG +IW     +P FN   N  + C+   
Sbjct: 125 LLKPWQYLGKCAQEKSS---------PFERAHGCEIWDLALADPKFNNFLNGAMQCSTTT 184

Query: 193 ITLPAILENCGDIFNGVE-SLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYD 252
           I    +LE   D F+G+  SLVDVGGG G+ ++ +VKA P ++GINFDLP+VV T+ ++ 
Sbjct: 185 IINEMLLE-YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFP 244

Query: 253 GVEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVI 312
           GV+HVGG+M   +P+ADA    WILH W DEDC  ILKNC  AI   K  GKVII+D V+
Sbjct: 245 GVKHVGGDMFVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAI-RKKKNGKVIIVDCVL 304

Query: 313 DENEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSI 363
              + N +   + L  D++MM  +  G+ERT  EW ++L+N AGF R  +   P A P I
Sbjct: 305 -RPDGNDLFDKMGLIFDVLMMAHTTAGKERTEAEW-KILLNNAGFPRYNVIRTP-AFPCI 353

BLAST of CsaV3_7G001370 vs. Swiss-Prot
Match: sp|B0ZB56|OMT2_HUMLU (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 5.5e-62
Identity = 148/371 (39.89%), Postives = 221/371 (59.57%), Query Frame = 0

Query: 12  KKEEEEAKIQ----IYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVLICS 71
           + ++ EA ++    ++K + G A+  V+KCA+EL+I +++ SH  P +TL+Q+++ +  S
Sbjct: 5   RNDQTEAALRGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAP-ITLAQIASSVPDS 64

Query: 72  PPL----LFRIMRFLTHRGIFKEEITPE-NLIAYSQTPLSHML---------INVAPLLL 131
           P L    L RIMR L  R IF +  + +   + Y  T  S +L         + +AP + 
Sbjct: 65  PSLNLSYLSRIMRLLVRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVTLAPFVA 124

Query: 132 LENSPEIVASWQNLSARLQNSYNNNDDSDLLVPFEVAH-GKDIWSYGARNPGFNRMFNEG 191
               P + A W  L+  ++   N          FE+ H G+ +W     NP FN++FN+G
Sbjct: 125 FMTHPYLSAPWSCLARCVKEGGNG---------FEMVHGGRQLWDLSPGNPEFNKVFNDG 184

Query: 192 LACNARVITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVL 251
           +A  AR+ T+ AIL    D+F G+ SLVDVGG  G ++S +VK+ P +KGIN+DLP+VV 
Sbjct: 185 MASTARITTM-AILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVA 244

Query: 252 TSEKYDG-VEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKV 311
           T+  Y G V HVGGNM +++P A A F  WILH W DEDC+KILKNC+ A+ E   GGK+
Sbjct: 245 TAPTYTGLVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEK--GGKI 304

Query: 312 IIIDSVIDENEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPI 363
           II+D V+ E E N +  D  + LDI +M  +R G+ERT  EW ++L  + GF R  I  I
Sbjct: 305 IIVDIVL-EPEGNGLFDDAAVMLDIALMALTR-GKERTEKEWKRVL-EEGGFPRYQILKI 358

BLAST of CsaV3_7G001370 vs. Swiss-Prot
Match: sp|Q9LEL6|6OMT_COPJA ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 3.0e-60
Identity = 143/360 (39.72%), Postives = 215/360 (59.72%), Query Frame = 0

Query: 12  KKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVLICSP--- 71
           KK+   ++ +++ +++GFAE+ V+KCA++L +AN+I + G  +MTLS+LS+ L   P   
Sbjct: 4   KKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSG-TSMTLSELSSRLPSQPVNE 63

Query: 72  PLLFRIMRFLTHRGIF-KEEITPENLIAYSQTPLSHMLIN-----VAPLLLLENSPEIVA 131
             L+R+MR+L H  +F K  I  E  + Y   P +  L+      +   +L     + +A
Sbjct: 64  DALYRVMRYLVHMKLFTKASIDGE--LRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMA 123

Query: 132 SWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVITL 191
            W  L   L               FE A G +IW Y A +P  N++FNE +A ++R+I +
Sbjct: 124 PWHYLKDGLSGESG--------TAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLI-M 183

Query: 192 PAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEH 251
            A+++ CG+IFNG+ +LVDVGGG GT +  +  AFP +K   +DLP+V+  S  Y  V  
Sbjct: 184 SALVKECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHC 243

Query: 252 VGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENE 311
           V G+M  F+PKADA     ILH WDD++CI+ILK CKEA+     GGKVII+D V++   
Sbjct: 244 VAGDMFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVK--GGKVIIVDIVLNVQS 303

Query: 312 ENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQAF 363
           E+   T +RLTLD+ MM  +  G+ERT +EW + LI+ AG+    IT I  AV S+I+A+
Sbjct: 304 EHPY-TKMRLTLDLDMMLNT-GGKERTEEEWKK-LIHDAGYKGHKITQI-TAVQSVIEAY 345

BLAST of CsaV3_7G001370 vs. Swiss-Prot
Match: sp|B0ZB55|OMT1_HUMLU (Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 5.2e-60
Identity = 140/359 (39.00%), Postives = 222/359 (61.84%), Query Frame = 0

Query: 21  QIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVL------ICSP--PLLF 80
           QI++ +F F ++  +KCAIEL+IA++I SHG+P +TLSQ+++ +        SP  P L 
Sbjct: 9   QIWQLMFSFVDSMALKCAIELRIADIIHSHGKP-ITLSQIASGIRSNSNSSISPNIPYLS 68

Query: 81  RIMRFLTHRGIFKE--EITPENLIAYSQTPLSHMLI-----NVAPLLLLENSPEIVASWQ 140
           RIMRFL  + IF E  E   E +  Y  +  S  L+     ++AP++L++  P  +A W 
Sbjct: 69  RIMRFLVRKNIFTEHQEDNDEVISLYGLSDSSRWLLRDFKSSLAPMVLMQTHPLSMAVWH 128

Query: 141 NLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVITLPAI 200
            L   ++NS N          FE AHG +IW + + NP FN++FN  +A +   I + A+
Sbjct: 129 FLEDYVRNSSNT---------FEKAHGCNIWEFSSANPDFNKIFNNAMA-SIVPIYMGAV 188

Query: 201 LENCGDIFNGVE-SLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHVG 260
           L +  D    ++ ++VDVGGG G ++S L+K +P +KGINFDLP+V+ T+   DGV H+ 
Sbjct: 189 LSSYKDGLGCIKGTVVDVGGGTGGSISELMKYYPNIKGINFDLPHVIATAPALDGVTHIS 248

Query: 261 GNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENEEN 320
           G++ + +P ADA     +LH + DE C+K+L+NC++AI + K  GK+II++ V+D    N
Sbjct: 249 GDIFESIPSADAVLMKGVLHCFSDEKCVKVLRNCRKAITDKK-NGKIIILEIVLDPT-SN 308

Query: 321 KMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQAFI 364
           ++  + R+  D+++   S  G+ERT  EW +LL N+AGF+   IT IP  +P+II+AF+
Sbjct: 309 QIFDETRMVYDLLIPXFS-GGKERTELEWKRLL-NEAGFTSIKITKIP-IIPAIIEAFL 351

BLAST of CsaV3_7G001370 vs. TrEMBL
Match: tr|A0A0A0K1F0|A0A0A0K1F0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G017710 PE=3 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 5.5e-208
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MEQTITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQL 60
           MEQTITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQL
Sbjct: 1   MEQTITKESIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQL 60

Query: 61  STVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEI 120
           STVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEI
Sbjct: 61  STVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPEI 120

Query: 121 VASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVI 180
           VASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVI
Sbjct: 121 VASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVI 180

Query: 181 TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV 240
           TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV
Sbjct: 181 TLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGV 240

Query: 241 EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE 300
           EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE
Sbjct: 241 EHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDE 300

Query: 301 NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQ 360
           NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQ
Sbjct: 301 NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSIIQ 360

Query: 361 AFIS 365
           AFIS
Sbjct: 361 AFIS 364

BLAST of CsaV3_7G001370 vs. TrEMBL
Match: tr|A0A1S4DWV8|A0A1S4DWV8_CUCME ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490399 PE=3 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 3.7e-127
Identity = 232/261 (88.89%), Postives = 239/261 (91.57%), Query Frame = 0

Query: 1   MEQTITKESIWKK-EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60
           ME+TITKES WKK               GFAEAAVIKCAIELKIANVIESHGRPTMTLSQ
Sbjct: 1   MEETITKESKWKKXXXXXXXXXXXXXXXGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60

Query: 61  LSTVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPE 120
           LST L CSPPLLFRIMRFLTHRGIF+EE TPENLIAYSQTPLSHMLINVAPLLLLENSPE
Sbjct: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120

Query: 121 IVASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARV 180
           IVASW NLSA+L+NSYNNN++SDL+VPFEVAHGKDIWSYGARNPGFNRMFNEGL CNARV
Sbjct: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180

Query: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDG 240
           ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVV TSEKYDG
Sbjct: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240

Query: 241 VEHVGGNMLDFVPKADAAFFM 261
           VEHVGGNMLDFVPKADAAFFM
Sbjct: 241 VEHVGGNMLDFVPKADAAFFM 261

BLAST of CsaV3_7G001370 vs. TrEMBL
Match: tr|A0A0A0K2N4|A0A0A0K2N4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G017720 PE=3 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 3.7e-111
Identity = 211/366 (57.65%), Postives = 276/366 (75.41%), Query Frame = 0

Query: 7   KESIWKK--EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVL 66
           ++SI KK  EEEEA+++I+KY+FGF E A++KCAIEL+I + IESHG P MTLSQLST L
Sbjct: 6   QKSIPKKQEEEEEARVEIWKYIFGFVEMAIVKCAIELRIGDTIESHGSP-MTLSQLSTAL 65

Query: 67  ICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLI-----NVAPLLLLENSPE 126
            CS  LL+RI+RFL  RGIFK+EI   N+I+Y QTPLS +L      ++AP LLLE+SP 
Sbjct: 66  NCSASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMAPFLLLESSPV 125

Query: 127 IVASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARV 186
           ++A W  LSAR++ +           PFE AHGKD+WS+ A +P  N + N+ ++C ARV
Sbjct: 126 MLAPWHRLSARIKGNGE--------TPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARV 185

Query: 187 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDG 246
           +T+PAILE C  IF G+ SLVDVGGGNGT LS++VKAFPW+KGINFDLP+V+ +S++Y G
Sbjct: 186 LTVPAILEECPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIG 245

Query: 247 VEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVID 306
           VEHVGGNMLD +PKADAAF MW+LH WDDE CIKILKNCK AI E +  GKVII++++I+
Sbjct: 246 VEHVGGNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKGAISEKR--GKVIIVEALIE 305

Query: 307 E--NEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPS 364
           E   E N    D+ L LD++MM  ++ G+ERT+ EW  +L ++AGF++ TITPI  AV S
Sbjct: 306 ERSEENNNKLGDVGLMLDMVMMAHTKNGKERTSKEWGHVL-HQAGFTQYTITPI-RAVHS 358

BLAST of CsaV3_7G001370 vs. TrEMBL
Match: tr|A0A0A0K0Q2|A0A0A0K0Q2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 7.6e-109
Identity = 217/370 (58.65%), Postives = 271/370 (73.24%), Query Frame = 0

Query: 1   MEQTITKESIWKKEEEE---AKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTL 60
           M+ T +K S   K EEE    K+QI+KY+FGF E AVIKCAIELKI +VIESHG  +MTL
Sbjct: 1   MDDTSSKASNKLKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHG-DSMTL 60

Query: 61  SQLSTVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLI-----NVAPLL 120
           SQLS+ L CSP LL RI+RFL HRGIFKEE T +    YS TP+S +L      ++APL 
Sbjct: 61  SQLSSSLNCSPLLLHRILRFLVHRGIFKEEKTTK---CYSHTPMSRLLTTTGPNSMAPLH 120

Query: 121 LLENSPEIVASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEG 180
           LLE+SP ++A W NLSA ++ S + N       PFE+AHG D+W+Y A NP  + + NE 
Sbjct: 121 LLESSPVMIAPWHNLSACVKASDHENGSQ----PFEMAHGMDLWTYAAANPSHSSLINES 180

Query: 181 LACNARVITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVL 240
           +AC AR++ LPA+LE CG+IF+GV  LVDVGGGNGT LS+LVKA PW+KGINFDLP+VV 
Sbjct: 181 MACFARLVILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVS 240

Query: 241 TSEKYDGVEHVGGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVI 300
            S++Y+G++HVGGNM D VPKADAAF M +LH WDDE+CIKILKNCKEAI E    GKVI
Sbjct: 241 VSKEYEGIQHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKT--GKVI 300

Query: 301 IIDSVIDENEENKMATDIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIP 360
           I++ VIDE EE    +D RL +D++MM  + KG+ERT +EW  +L  KAGFS  TITP  
Sbjct: 301 IVEVVIDEKEEISKYSDARLMMDMIMMAHTIKGKERTNEEWANVL-QKAGFSYYTITP-T 358

Query: 361 AAVPSIIQAF 363
           + + S+IQAF
Sbjct: 361 STIQSVIQAF 358

BLAST of CsaV3_7G001370 vs. TrEMBL
Match: tr|A0A1S3BII4|A0A1S3BII4_CUCME ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490252 PE=3 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 6.5e-108
Identity = 209/364 (57.42%), Postives = 262/364 (71.98%), Query Frame = 0

Query: 9   SIWKKEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTVLICSP 68
           SI KKEEEEA++QI+KY+FGF E A++KCAIEL+I + IESHG P MTLSQLST L CS 
Sbjct: 8   SISKKEEEEARVQIWKYIFGFVEMAIVKCAIELRIGDTIESHGSP-MTLSQLSTALNCSS 67

Query: 69  PLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLI-----NVAPLLLLENSPEIVAS 128
            LL+RI+RFL  RGIFK+EIT EN+I+Y  TPLS +L      ++APLLLLE+SP ++A 
Sbjct: 68  SLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMAPLLLLESSPVMLAP 127

Query: 129 WQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARVITLP 188
           W  LSAR++ +           PFE AHGKD+WS+ A +P  N + N+ ++C ARV T+P
Sbjct: 128 WHRLSARIKGNGE--------TPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVHTVP 187

Query: 189 AILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDGVEHV 248
           AILE+C  IF G+ SLVDVGGGNGT LS++VKAFPW+KGINFDLP+V+ +S++Y GVEHV
Sbjct: 188 AILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHV 247

Query: 249 GGNMLDFVPKADAAFFMWILHAWDDEDCIKILKNCKEAIGENKAGGKVIIIDSVIDENEE 308
           GGNMLD +PKADAAF MW+LH WDDE CIKILKNCKEAI E +  GKV            
Sbjct: 248 GGNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKEAISEKR--GKVXXXXXXXXXXXX 307

Query: 309 NKMAT----DIRLTLDIMMMTRSRKGRERTADEWTQLLINKAGFSRCTITPIPAAVPSII 364
                    D+ L LD++MM  +  G+ERT  EW  +L  +AGF+R TITPI  AV S+I
Sbjct: 308 XXXXXXXLGDVGLMLDMVMMAHTENGKERTIKEWGNVL-RQAGFTRYTITPI-RAVHSVI 358