Cla97C03G053540 (gene) Watermelon (97103) v2

NameCla97C03G053540
Typegene
OrganismCitrullus lanatus (Watermelon (97103) v2)
DescriptionFAD-binding Berberine family protein
LocationCla97Chr03 : 2722998 .. 2728085 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAGTCATATGGGAAACTACTCAAGTTTTGTCTTTCTTCTTCTTCTGTCCTTTCTCGTATGGGATTGTTCAGGTGACAATCTTCACAATTTTCTCCATTGTGTTTCTCCTCATTCCTCTCAGCTGGACGATTCAAATTCAACCTTCATTTACACTCCATCCTCTCCTTCTTACTCTTCCGTTCTAAATTTCACCATTCGAAACCTTAGATTCTCCGGACCCAACATCCCTAAACCCATCGCCATAATCAAACCATCACAACCTTCCCAAGTTCAAACAGCCCTCATTTGTTGCAAGAAGCATGGCATCCACATTCGAATTCGCAGCGGCGGCCACGACTTCGAGGGTCTTTCTTACGTCGCCAACCTCCCATTTCTACTCATTGATTTGGCCAATCTTAATGCGGTAACGGTTGAGGACGACAACACGGCATGGGTTCAATCAGGGGCGAGCATGGGGGAACTCTACTACAAGCTCAGCCAACAAACGTCATCCTTTGGGTTCCCTGGGGGAGTGCAGCCGAATGTTGGGGTCGGCGGGTTCTTTAGCGGCGGTGGGTACGGGTTGATGGTTAGAAAATATGGTCTTGGGGTGGATAATGTGGTGGATGCCCAACTTGTGGATGCAAATGGGGATCTTCTTGATAGGAAGTCGATGGGGGAGGATTTGTTTTGGGCCATTAGAGGGGGTGGAGGAGGAAGCTTTGGAATTGTTTTGGCTTGGAAGTTGAAGTTGGTTCCTGTTCCTGCCATTGTTACGTCCTTTACTGCTTTTAGAAGTTGGGGCCCAAACTCAACCAAGTTGGTCCATCGGTGGCAGTATGTAGCACCAGAGATTGACCATAGATTATTCATTGGCATCATGGTCACAGGTTTATATATATTCCTTCTCTTTCACTTTAATATCAAACTGCATGTCAAATTTAACACTCTATTTCATAATTATTTAGGTCTAGTCCCGTAATTATTTGGTTTTTTATTTCATATTTTTTACTTTTAAATTTTTTGTTTTGTTTACTTTTTTTTTATTGATGTTTAAAAAAATCAAATCAAATTATAAAATATTAAAAAAAAAAAGAGTAGTTTTTAAGCCTTTGTTTTTATTTTTGAGTATTCAAGACAGAAGACAGAAGCAAAACATTGTAAGAAATGAAGAGAAATTGACTTAATTTTCAAAAACAAAAAACAAAATGTTTGTCAAACTAGACCTTAGATTTCGTTATCACTATTCTGTTTTTTAAATATATGAATGTTTCTTTCCAATTTCTCTATTAAGTTTTTTTTTTTTTTTTCTCTCTCTCTCTCTTAAAGACAAAGTACAAATCTTAGTTTTTAAGTTATAAAACCAAAAATAAATTTTCGAAAACTACGAACCTTTTTAGTAACCATCTCAGTTGGTGGGTGTTTTTCTATTTTTAATTTTTGTTTTTTTAATAAAAAAAATTATATATGTTTGATAATTGTTGTTTTTGTTTCATGTGTTATTAAAAAACAAAAAATCATGAGCTGTTGGGACAGTTACGGCTTTGAGTTTTTAAAAATAAAAAGGAAAAGAACTAAAGAATTATTTGATTTTTGGTTTACTATAAAATTGATAGAAAACTATTTTTATAACATTGTCTGGCCTCCTATATGTATGTATGGGCCAAAATCTGCTTTAATTGTTTCGAACATTACAATAATGTAACCACTTTATCTTTTCGAACTTTACAATCTTGTAATCAATTTGTCTTTCTTCAGTAATCAATTAAGGGACAAATAATAAAAGAAACATAGGTTAGAGACACGAATTTAATTTATCAAATAATTTTTCAAAATATAAGGTCCAATTTTAAGAATTTGACCGTTTAAGGTAGAAATTGAATTTCTCTAAAATAAAAAAGACAAAGTGATGGAATAATTTTGTCCACATTTCAAAAGAACAGAGACTCTTTAATTGGTGGTTATCATTTTTGGAATTATGTAGGTAAAGAATTTTGTCGGTAAAAGGGACAAAAATGTTACACACGTCATATGTTAGTGTAAATTATAATATATAACAATAGTATCAATTAGAAAATAATATAGAAATATTATTTTAAATGATAAAATTTCTGAAAATATTGACATTTAATAGCGAAATTACACAATTTATCTGTGATATACTGCAATAGATCGTGATAAACTACTATATGTGTCTATCATGACACAAATAGTAGTCTATCACAGATTAACATTAAAATTTTGCTATATTTATAAATATTTTGATTACTTTTGTTATATTAAAATAGTCCAATAATATTTTTGACAGATTGGTTTTGTGTATAAAATAACAATATAATATTATAATAATTTAATATAGTAGACCAATTGTAATATAATATGACTGATATAATATTATAATACATTAAAAAATCTTGGGATAAAATTACACCATCCACTATAATACTATAATATGGCAGACATGATTTCTTTTTTCTTTTTTTTCTTTGTTTTAACAGTATAATATAGCAGACAGGATGTAGTTTTCAATTTCTTTTTCTTGTTTTTTTTTTTCTTCTGAATCTAAAAACGTTTTTAAAAATTTATAAACGATAGATGGAATAGATAATCAAATGTTTTCTCTTATAAAACTAAAAACAAAATTGGTTAGACTAAATCTTACACTTTTCAGTTTTTAAAAATTTCAAAATTTGACTTAGATTTTTAAAGTATTGTAGAATTTTATAGGTGGAATTAGTATTCATAAACTTAATTTTCAAAATCTAAAAACTAAATGTTGTCAAGTGGGGCCTTACACTAAAACAACATGTACATTTTATGACACATGAATAGTCTCATGTAAATAGGAGAGATAAAAAGCCGTCATTAAAGCTAATTTCACCTGTTTTTTTGTGGGAAAAAAATGGAGTTTTATGAATTTTTTAAAATTTATGACTGTTTTTTTTGTCACGAATTCATAACGGTAAATAAAAGTCATTAAATGTAGGTTTTTATTTTAAGTTTTTGAAAATGATGCTTATAATTATACACTATTCCCTCTTATAAGTTTTTGTTTTGTTTTCTTTTTGTTATTTACTTTAATCATTGTTTTACTAAACCAATCCAAATCTTAAATTTTTTTAAAAAATTGTTTTTATTTTTGACATTTTACTAAGAATTTAAATATTTATTTAAAAAACTTGTTATCTAGTGAAGGTTAAAATTTTAGGTTTTTGTTTATGAAGTATAAAAAGTGTAAAATTCCTAGATTTTTAAGCTTGTGCCTAAACTTTAAAAAATGTCAGATAAGTTCATAAACTTTCAATATTTTATTTTATTTTTTTATCCATAGATTTTGAATTTTGAATTTTGTTCATAGTTAATGACTCTTACCTATATACAACATTTTTTTTAAATATAATTTCAGACTCACAAGATATTAAATTCAAAGTTTAAAGGTTGCATTATGCATAGAATTCTAAACGCAATTAATTTCAATAGACCATGTTTAGTAATTATTAGTTTTTAGTTTTTAGTTTTTGAAAAAGTATATTTTCTCTCAACTTTGCAATAATTTTTATGTTTCTTAAGTAAAAGAGTTAAATTCTTAACCAAATTTTAAATTCCAAAAGCTACTTTAAAAATTATAAACTATTTTTTTAGTTTTCAAAACTTGGTTTAAATTTTTAAAACATTGATAGAAAATAGATGACAATGGAAGAAATTTAGAGTTTTATAAGTCTAAATTTCAAAAACTAAATAGTTACCCAATGAAGTTATAAATTTAAATTTTAAATATATGATTGCTTTTAACCGTAGATAATTGAATGGACCAATTGAATACTAGTTAAAATTAAGATACCAAAATGGATACAATTACCTTTAAAATTGATAGATTGTTTTTGTAACTTATTTTTTTTTTTTTTTTTTTTAAGGAAAACAAACAACAAATCAAACTAGGAAGACAAATAAGGATTCTTAACCAAGTTCAAATTAATATTTTTGTCCTTGTAGAAAACTCAACATCAAAAAGGCTCTCACTCATTAATTATTATTTTATTTTTTAAAATTAAAAAGTGAAAAAATACTTAGCTACATCTTGATACTTCATACTACACCATATCTTTGTATAGCCTACTCAATTGTTAGTTCCCACTGTCAAAATGTTTTTCTTTTTTTTCATTTTTTTTAAATGTTATTTTACTTTTGACTATTGATTTTTTTTTTTTTTTTAATTGATTGAAGTAGACTACACAAATATGGATATAATGGAGATTTAGATTGATAAATGTGATGCTAAAAGTATATATAATTTGTAATTTGTAATCTTCACACAGGTGCAAATTCAGGAGGAGGAAGAATAATGGGAGTATTTATGTACTCTCTATTTTTAGGAAGGGCAGAGGAGCTTATTTCCATCATGGACAAGAATTTTCCAGAACTTGGATTGAAAAAAGAGGATTGTGTTGAATCCAATTGGGTTGAATCAATGGCATATCCAGCAAGTGGGTTTGTCACAACAAAGGATATGAATTTCTTGCTAGATAGAACCCCACTGAAGAATGGCAGATACAAAACCAAATCTGATTATGCAACTAAACCCATTTCAGAAACTGCCCTTGAAGGCATATGGGAAAGGTTCAATGCCAAAGGAATAGAAACAGTGCAACTCGTACTGATCCCTTATGGAGGCAAAATGAATGAATTTTCTGAAACAGATACTCCTTCCCCACATAGAGCTGGATATCCCATCAAAATTGCTTATTATGTCACATGGAATTCACCCCAAGACGACTCGAGACACTTGGAGTGGACCCGAGAGCTCTACGACTACATGGCTCCATTTGTGTCGAAATCGCCTAGGGCTGCATATGTCAATTATAGAGATCTTGACATCGGAAGAAACAATGAGGACGGAATCCCGACCAGCTACGAGGAAGCGAGCGTTTGGGGGTTGAAGTATTTTGGGGATAATTTTGAGAGGCTGGTGGAAGTGAAAACGAAGGTGGATCCCTTTAACTTATTCCGGCATGAACAGAGCATACCACCAGTTGCTCGTGGAGGAGCCCAAGCAAAGATGTCTTCATGCCCCGATGTGTCCTACAATGCAATGCAATATCTATAA

mRNA sequence

ATGGATAGTCATATGGGAAACTACTCAAGTTTTGTCTTTCTTCTTCTTCTGTCCTTTCTCGTATGGGATTGTTCAGGTGACAATCTTCACAATTTTCTCCATTGTGTTTCTCCTCATTCCTCTCAGCTGGACGATTCAAATTCAACCTTCATTTACACTCCATCCTCTCCTTCTTACTCTTCCGTTCTAAATTTCACCATTCGAAACCTTAGATTCTCCGGACCCAACATCCCTAAACCCATCGCCATAATCAAACCATCACAACCTTCCCAAGTTCAAACAGCCCTCATTTGTTGCAAGAAGCATGGCATCCACATTCGAATTCGCAGCGGCGGCCACGACTTCGAGGGTCTTTCTTACGTCGCCAACCTCCCATTTCTACTCATTGATTTGGCCAATCTTAATGCGGTAACGGTTGAGGACGACAACACGGCATGGGTTCAATCAGGGGCGAGCATGGGGGAACTCTACTACAAGCTCAGCCAACAAACGTCATCCTTTGGGTTCCCTGGGGGAGTGCAGCCGAATGTTGGGGTCGGCGGGTTCTTTAGCGGCGGTGGGTACGGGTTGATGGTTAGAAAATATGGTCTTGGGGTGGATAATGTGGTGGATGCCCAACTTGTGGATGCAAATGGGGATCTTCTTGATAGGAAGTCGATGGGGGAGGATTTGTTTTGGGCCATTAGAGGGGGTGGAGGAGGAAGCTTTGGAATTGTTTTGGCTTGGAAGTTGAAGTTGGTTCCTGTTCCTGCCATTGTTACGTCCTTTACTGCTTTTAGAAGTTGGGGCCCAAACTCAACCAAGTTGGTCCATCGGTGGCAGTATGTAGCACCAGAGATTGACCATAGATTATTCATTGGCATCATGGTCACAGGTGCAAATTCAGGAGGAGGAAGAATAATGGGAGTATTTATGTACTCTCTATTTTTAGGAAGGGCAGAGGAGCTTATTTCCATCATGGACAAGAATTTTCCAGAACTTGGATTGAAAAAAGAGGATTGTGTTGAATCCAATTGGGTTGAATCAATGGCATATCCAGCAAGTGGGTTTGTCACAACAAAGGATATGAATTTCTTGCTAGATAGAACCCCACTGAAGAATGGCAGATACAAAACCAAATCTGATTATGCAACTAAACCCATTTCAGAAACTGCCCTTGAAGGCATATGGGAAAGGTTCAATGCCAAAGGAATAGAAACAGTGCAACTCGTACTGATCCCTTATGGAGGCAAAATGAATGAATTTTCTGAAACAGATACTCCTTCCCCACATAGAGCTGGATATCCCATCAAAATTGCTTATTATGTCACATGGAATTCACCCCAAGACGACTCGAGACACTTGGAGTGGACCCGAGAGCTCTACGACTACATGGCTCCATTTGTGTCGAAATCGCCTAGGGCTGCATATGTCAATTATAGAGATCTTGACATCGGAAGAAACAATGAGGACGGAATCCCGACCAGCTACGAGGAAGCGAGCGTTTGGGGGTTGAAGTATTTTGGGGATAATTTTGAGAGGCTGGTGGAAGTGAAAACGAAGGTGGATCCCTTTAACTTATTCCGGCATGAACAGAGCATACCACCAGTTGCTCGTGGAGGAGCCCAAGCAAAGATGTCTTCATGCCCCGATGTGTCCTACAATGCAATGCAATATCTATAA

Coding sequence (CDS)

ATGGATAGTCATATGGGAAACTACTCAAGTTTTGTCTTTCTTCTTCTTCTGTCCTTTCTCGTATGGGATTGTTCAGGTGACAATCTTCACAATTTTCTCCATTGTGTTTCTCCTCATTCCTCTCAGCTGGACGATTCAAATTCAACCTTCATTTACACTCCATCCTCTCCTTCTTACTCTTCCGTTCTAAATTTCACCATTCGAAACCTTAGATTCTCCGGACCCAACATCCCTAAACCCATCGCCATAATCAAACCATCACAACCTTCCCAAGTTCAAACAGCCCTCATTTGTTGCAAGAAGCATGGCATCCACATTCGAATTCGCAGCGGCGGCCACGACTTCGAGGGTCTTTCTTACGTCGCCAACCTCCCATTTCTACTCATTGATTTGGCCAATCTTAATGCGGTAACGGTTGAGGACGACAACACGGCATGGGTTCAATCAGGGGCGAGCATGGGGGAACTCTACTACAAGCTCAGCCAACAAACGTCATCCTTTGGGTTCCCTGGGGGAGTGCAGCCGAATGTTGGGGTCGGCGGGTTCTTTAGCGGCGGTGGGTACGGGTTGATGGTTAGAAAATATGGTCTTGGGGTGGATAATGTGGTGGATGCCCAACTTGTGGATGCAAATGGGGATCTTCTTGATAGGAAGTCGATGGGGGAGGATTTGTTTTGGGCCATTAGAGGGGGTGGAGGAGGAAGCTTTGGAATTGTTTTGGCTTGGAAGTTGAAGTTGGTTCCTGTTCCTGCCATTGTTACGTCCTTTACTGCTTTTAGAAGTTGGGGCCCAAACTCAACCAAGTTGGTCCATCGGTGGCAGTATGTAGCACCAGAGATTGACCATAGATTATTCATTGGCATCATGGTCACAGGTGCAAATTCAGGAGGAGGAAGAATAATGGGAGTATTTATGTACTCTCTATTTTTAGGAAGGGCAGAGGAGCTTATTTCCATCATGGACAAGAATTTTCCAGAACTTGGATTGAAAAAAGAGGATTGTGTTGAATCCAATTGGGTTGAATCAATGGCATATCCAGCAAGTGGGTTTGTCACAACAAAGGATATGAATTTCTTGCTAGATAGAACCCCACTGAAGAATGGCAGATACAAAACCAAATCTGATTATGCAACTAAACCCATTTCAGAAACTGCCCTTGAAGGCATATGGGAAAGGTTCAATGCCAAAGGAATAGAAACAGTGCAACTCGTACTGATCCCTTATGGAGGCAAAATGAATGAATTTTCTGAAACAGATACTCCTTCCCCACATAGAGCTGGATATCCCATCAAAATTGCTTATTATGTCACATGGAATTCACCCCAAGACGACTCGAGACACTTGGAGTGGACCCGAGAGCTCTACGACTACATGGCTCCATTTGTGTCGAAATCGCCTAGGGCTGCATATGTCAATTATAGAGATCTTGACATCGGAAGAAACAATGAGGACGGAATCCCGACCAGCTACGAGGAAGCGAGCGTTTGGGGGTTGAAGTATTTTGGGGATAATTTTGAGAGGCTGGTGGAAGTGAAAACGAAGGTGGATCCCTTTAACTTATTCCGGCATGAACAGAGCATACCACCAGTTGCTCGTGGAGGAGCCCAAGCAAAGATGTCTTCATGCCCCGATGTGTCCTACAATGCAATGCAATATCTATAA

Protein sequence

MDSHMGNYSSFVFLLLLSFLVWDCSGDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVEDDNTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPNSTKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIPPVARGGAQAKMSSCPDVSYNAMQYL
BLAST of Cla97C03G053540 vs. NCBI nr
Match: XP_022964765.1 (berberine bridge enzyme-like 26 [Cucurbita moschata])

HSP 1 Score: 886.3 bits (2289), Expect = 5.2e-254
Identity = 439/551 (79.67%), Postives = 481/551 (87.30%), Query Frame = 0

Query: 7   NYSSFVFLLLLSFLVWDCSGDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFT 66
           NY + V LLL  F + D  G NL +FL CVS      DDSN   I TPSS +YSSVLNFT
Sbjct: 6   NYLNLVTLLLF-FAISDSFGGNLDDFLRCVSN-----DDSNPILINTPSSSTYSSVLNFT 65

Query: 67  IRNLRFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVAN-LP 126
           +RNLRFSGP+ PKP AII PSQ S VQ  +IC K HG+HIR+RSGGHDFEGLSYVA+ +P
Sbjct: 66  VRNLRFSGPDTPKPFAIINPSQASHVQGGVICAKNHGVHIRVRSGGHDFEGLSYVAHGVP 125

Query: 127 FLLIDLANLNAVTVE-DDNTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFS 186
           F+LID+A LN+V+V+ DDNTAWV+SGASMGELYYKL  +TS FGFPGGVQPNVGVGGF S
Sbjct: 126 FVLIDMAKLNSVSVDTDDNTAWVESGASMGELYYKLGLETSGFGFPGGVQPNVGVGGFLS 185

Query: 187 GGGYGLMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKL 246
           GGGYGLMVRKYGL VDNVVDAQ+VDANG L+DR SMGEDLFWAIRGGGGGSFGIV+AWKL
Sbjct: 186 GGGYGLMVRKYGLAVDNVVDAQVVDANGRLVDRDSMGEDLFWAIRGGGGGSFGIVVAWKL 245

Query: 247 KLVPVPAIVTSFTAFRSWGPNSTKLVHRWQYVAPEIDHRLFIGIMVTGANS--GGGRIMG 306
           KLVPVP+IVTSFTAFR W  NST LVH+WQ VAPEID RLFIG MVT ANS  GGGRIMG
Sbjct: 246 KLVPVPSIVTSFTAFRIWDQNSTNLVHKWQLVAPEIDERLFIGAMVTAANSGEGGGRIMG 305

Query: 307 VFMYSLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDR 366
           VF+YSLFLGRAEE+ISIM+ NFPE+GL+ EDCVESNWVESMAYPASGFVT KDMNFLLDR
Sbjct: 306 VFLYSLFLGRAEEVISIMENNFPEVGLRMEDCVESNWVESMAYPASGFVTAKDMNFLLDR 365

Query: 367 TPLKNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPS 426
           TPL NGRYKTKSDYAT+PIS+TAL GIWERFNAKGI+TVQLVLIPYGGKMN+FSETDTPS
Sbjct: 366 TPLTNGRYKTKSDYATEPISQTALHGIWERFNAKGIQTVQLVLIPYGGKMNKFSETDTPS 425

Query: 427 PHRAGYPIKIAYYVTWNSPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNN 486
           PHRA YPIKIAYYVTW++ + DSRHLEWTRELYDYM PFVSKSP+AAYVNYRDLDIGRNN
Sbjct: 426 PHRARYPIKIAYYVTWDTLEGDSRHLEWTRELYDYMTPFVSKSPKAAYVNYRDLDIGRNN 485

Query: 487 EDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIPPVARGGAQAKMSSC 546
           E+GIPTSYEEA VWGLKYFGDNFERLVEVKTKVDP+NLFRHEQSIPPVA   AQ K+SS 
Sbjct: 486 EEGIPTSYEEAGVWGLKYFGDNFERLVEVKTKVDPYNLFRHEQSIPPVA---AQVKISSG 545

Query: 547 PDVSYNAMQYL 554
            DV+ + M+YL
Sbjct: 546 NDVACDKMEYL 547

BLAST of Cla97C03G053540 vs. NCBI nr
Match: XP_023521064.1 (berberine bridge enzyme-like 26 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 880.9 bits (2275), Expect = 2.2e-252
Identity = 432/531 (81.36%), Postives = 471/531 (88.70%), Query Frame = 0

Query: 28  NLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPS 87
           NL +FL CVS      DDSN   I TPSS +YSSVLNFT+RNLRFSGPN PKP AII PS
Sbjct: 3   NLDDFLRCVSN-----DDSNPILINTPSSSTYSSVLNFTVRNLRFSGPNTPKPCAIINPS 62

Query: 88  QPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVAN-LPFLLIDLANLNAVTVE-DDNTA 147
           Q S VQ  +IC K HG+HIR+RSGGHDFEGLSYVA+ + F+LID+A LN+V+V+ DDNTA
Sbjct: 63  QASHVQAGVICAKNHGVHIRVRSGGHDFEGLSYVAHGVAFVLIDMAKLNSVSVDTDDNTA 122

Query: 148 WVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDA 207
           WV+SGASMGELYYKL  +TS FGFPGGVQPNVGVGGF SGGGYGLMVRKYGL VDNVVDA
Sbjct: 123 WVESGASMGELYYKLGLETSGFGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDNVVDA 182

Query: 208 QLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPN 267
           Q+VDANG ++DR SMGEDLFWAIRGGGGGSFGIV+AWKLKLVPVP+IVTSFTAFR W  N
Sbjct: 183 QVVDANGRVVDRDSMGEDLFWAIRGGGGGSFGIVVAWKLKLVPVPSIVTSFTAFRIWDQN 242

Query: 268 STKLVHRWQYVAPEIDHRLFIGIMVTGANS---GGGRIMGVFMYSLFLGRAEELISIMDK 327
           ST LVH+WQ VAPEID RLFIG MVT ANS   GGGRIMGVF+YSLFLGRAEE+ISIM+ 
Sbjct: 243 STNLVHKWQLVAPEIDERLFIGAMVTAANSGEGGGGRIMGVFLYSLFLGRAEEVISIMEN 302

Query: 328 NFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPIS 387
           NFPE+GL+ EDCVESNWVESMAYPASGFVT KDMNFLLDRTPL NGRYKTKSDYAT+PIS
Sbjct: 303 NFPEVGLRMEDCVESNWVESMAYPASGFVTAKDMNFLLDRTPLTNGRYKTKSDYATEPIS 362

Query: 388 ETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQ 447
           +TAL GIWERFNAKGI+TVQLVLIPYGGKMN+FSETDTPSPHRA YPIKIAYYVTW++P+
Sbjct: 363 QTALHGIWERFNAKGIQTVQLVLIPYGGKMNKFSETDTPSPHRARYPIKIAYYVTWDTPE 422

Query: 448 DDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFG 507
            DSRHLEWTRELYDYM PFVSKSPRAAYVNYRDLDIGRNNE+GIPTSYEEASVWGLKYFG
Sbjct: 423 GDSRHLEWTRELYDYMTPFVSKSPRAAYVNYRDLDIGRNNEEGIPTSYEEASVWGLKYFG 482

Query: 508 DNFERLVEVKTKVDPFNLFRHEQSIPPVARGGAQAKMSSCPDVSYNAMQYL 554
           DNFERLVEVKTKVDP+NLFRHEQSIPPVA   AQ K+SS  DV+ + M+YL
Sbjct: 483 DNFERLVEVKTKVDPYNLFRHEQSIPPVA---AQVKISSGNDVACDKMEYL 525

BLAST of Cla97C03G053540 vs. NCBI nr
Match: XP_023525672.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 814.3 bits (2102), Expect = 2.5e-232
Identity = 394/534 (73.78%), Postives = 448/534 (83.90%), Query Frame = 0

Query: 7   NYSSFVFLLLLSFLVWDCSGDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFT 66
           NY +F+  +L+S L+W C G NL +FLHC+S +S     SNST +YT SS SYSSVLNFT
Sbjct: 6   NYVNFI-SVLVSLLLWGCWGSNLEDFLHCISSNS-----SNSTLLYTQSSSSYSSVLNFT 65

Query: 67  IRNLRFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPF 126
           IRNLRFSGPN+PKPIAIIKPSQPSQVQ A+ICC  H + IR+RSGGHDFEGLSYVAN+ F
Sbjct: 66  IRNLRFSGPNVPKPIAIIKPSQPSQVQAAVICCNNHDLRIRVRSGGHDFEGLSYVANVTF 125

Query: 127 LLIDLANLNAVTVE-DDNTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSG 186
           ++IDL NLN+VTV+ ++NTAWVQSGA+ GE+YYKL  ++S+FGFPGGVQP VG+GGF SG
Sbjct: 126 VVIDLVNLNSVTVDIENNTAWVQSGATQGEVYYKLGLKSSAFGFPGGVQPTVGIGGFLSG 185

Query: 187 GGYGLMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLK 246
           GGYG+MVRKYGLG DNVVDA LVDANG LLDR SMGEDLFWAIRGGGGGSFGIVLAWKLK
Sbjct: 186 GGYGMMVRKYGLGADNVVDALLVDANGRLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLK 245

Query: 247 LVPVPAIVTSFTAFRSWGPNSTKLVHRWQYVAPEIDHRLFIGIMVTG-------ANSGGG 306
           LVPVP  VTSF A+RS   N+T LVHRWQ+VAP ID RLFIG++V+G             
Sbjct: 246 LVPVPPTVTSFAAYRSLDQNATDLVHRWQFVAPVIDERLFIGVIVSGLXXXXXXXXXXXX 305

Query: 307 RIMGVFMYSLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNF 366
              G F YSLFLG+AEELI IM+K+FPELGLKK DC+E+NWVES AYPASGFVT  D+N 
Sbjct: 306 XXKGAFFYSLFLGKAEELIPIMEKSFPELGLKKADCLETNWVESTAYPASGFVTANDLNV 365

Query: 367 LLDRTPLKNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSET 426
           LLDR PL +GR+KTKSDY T+PISE AL+GIWER +A GIETVQLVLIPYGGKMNE +ET
Sbjct: 366 LLDRDPLSSGRFKTKSDYITEPISEAALDGIWERLDADGIETVQLVLIPYGGKMNEIAET 425

Query: 427 DTPSPHRAGYPIKIAYYVTWNSPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDI 486
           + PSPHR GYPIKI YYVTW SP+ D RHLEW+RE+Y+YMAPFVSKSPRAAYVNYRDLDI
Sbjct: 426 ELPSPHRDGYPIKIGYYVTWESPEADMRHLEWSREVYEYMAPFVSKSPRAAYVNYRDLDI 485

Query: 487 GRNNEDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIPPVAR 533
           G NNEDG PTS+EEASVWGLKYFG NFERLV VKT VDP NLFRH+QSIPP+A+
Sbjct: 486 GTNNEDGTPTSFEEASVWGLKYFGVNFERLVRVKTMVDPCNLFRHQQSIPPMAQ 533

BLAST of Cla97C03G053540 vs. NCBI nr
Match: XP_022964764.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 784.6 bits (2025), Expect = 2.1e-223
Identity = 376/547 (68.74%), Postives = 443/547 (80.99%), Query Frame = 0

Query: 7   NYSSFVFLLLLSFLVWDCSGDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFT 66
           N   F+ LLLLSF  W C  DNL +FLHC+S HSS    SNST I+TPSS SY  VLNF+
Sbjct: 27  NIEDFISLLLLSFASWVCGSDNLQDFLHCISLHSSN-HHSNSTLIHTPSSSSYPHVLNFS 86

Query: 67  IRNLRFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPF 126
           IRNLRF+  + PKP+AI+ P QPS+VQ  +ICCK HG+ IR RSGGHDFEG SYV+N+PF
Sbjct: 87  IRNLRFAESHTPKPVAIVTPWQPSEVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNVPF 146

Query: 127 LLIDLANLNAVTVEDDN-TAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSG 186
           +LIDL NLN++T++ DN TAW QSGA++GELYYK+ +++ +  FP G   ++G+GGFFSG
Sbjct: 147 VLIDLINLNSITIDVDNETAWAQSGATVGELYYKIGEKSRTLAFPAGFTASIGLGGFFSG 206

Query: 187 GGYGLMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLK 246
           GG G++VRKYGL VDNVVDA LVDANG LLDR SMGEDLFWAIRGGGGGSFGIVLAWKLK
Sbjct: 207 GGLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLK 266

Query: 247 LVPVPAIVTSFTAFRSWGPNSTKLVHRWQYVAPEIDHRLFIGIMVTGANS----GGGRIM 306
           LV VP  VTSF   +SW  N+ KL+HRWQYVAP++D  LFI   VT +NS    GGGR+M
Sbjct: 267 LVRVPPTVTSFAVHKSWDLNAAKLIHRWQYVAPKVDEDLFITAWVTASNSSEGEGGGRVM 326

Query: 307 GVFMYSLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLD 366
               +SLFLG+A EL+S+M K FPEL LKKEDC+E +WVE+MA+ AS +V+ +++  LLD
Sbjct: 327 KASFFSLFLGKAAELVSLMKKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLD 386

Query: 367 RTPLKNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTP 426
           RTPL  GRYK KSDYATKPISETAL GIWERF  + +ETVQL+LIP+GGKMNE SET TP
Sbjct: 387 RTPLITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISETATP 446

Query: 427 SPHRAGYPIKIAYYVTWNSPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRN 486
           +PHRA +PI I YYVTW+ P  DSRHLEWTREL+DYM PFVS+SPRAAY+NY+DLDIGRN
Sbjct: 447 NPHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGRN 506

Query: 487 NEDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIPPVA-RGGAQAKMS 546
           NEDGIPTSY+EASVWG +YFG NFERLVEVKTKVDPFN F+HE+SIPP A  G  QAKM+
Sbjct: 507 NEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFQHEESIPPAAPMGVIQAKMA 566

Query: 547 SCPDVSY 548
           SCPDVSY
Sbjct: 567 SCPDVSY 572

BLAST of Cla97C03G053540 vs. NCBI nr
Match: XP_023521065.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 780.8 bits (2015), Expect = 3.1e-222
Identity = 375/542 (69.19%), Postives = 442/542 (81.55%), Query Frame = 0

Query: 10  SFVFLLLLSFLVWDCSGDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRN 69
           SF+ LLLLSF  W C G +L +FLHC+S HSS    SNST I+TPSS SY  VLNF+IRN
Sbjct: 9   SFISLLLLSFASWGC-GSHLEDFLHCISLHSSN-HHSNSTLIHTPSSSSYPYVLNFSIRN 68

Query: 70  LRFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLI 129
           LRF+  + PKP+AI+ P Q SQVQ  +ICCK HG+ IR RSGGHDFEG SYV+N+PF+LI
Sbjct: 69  LRFAESHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNVPFVLI 128

Query: 130 DLANLNAVTVE-DDNTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGY 189
           DL NLN++T++ DD TAW QSGA++GELYYK+ +++ +  FP G   ++G+GGFFSGGG 
Sbjct: 129 DLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGFTASIGLGGFFSGGGL 188

Query: 190 GLMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVP 249
           G++VRKYGL VDN+VDA LVDANG LLDR SMGEDLFWAIRGGGGGSFGIVLAWKLKLV 
Sbjct: 189 GMLVRKYGLAVDNMVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVR 248

Query: 250 VPAIVTSFTAFRSWGPNSTKLVHRWQYVAPEIDHRLFIGIMVTGANS--GGGRIMGVFMY 309
           VP  VTSF   +SW  N+ KL+HRWQYVAPE+D  LFI   VT +NS  GGGR+M    +
Sbjct: 249 VPPTVTSFAIHKSWDLNAAKLIHRWQYVAPEVDEDLFITARVTASNSSYGGGRVMKASFF 308

Query: 310 SLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLK 369
           SLFLG+  EL+S+M K FPEL LKKEDC+E +WVE+MA+ AS +V+ +++  LLDRT L 
Sbjct: 309 SLFLGKVAELVSLMGKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTSLI 368

Query: 370 NGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRA 429
            GRYK KSDYATKPISETAL GIWERF  + +E VQL+LIP+GGKMNE SETDTP+PHRA
Sbjct: 369 TGRYKMKSDYATKPISETALNGIWERFKIEELEKVQLILIPFGGKMNEISETDTPNPHRA 428

Query: 430 GYPIKIAYYVTWNSPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGI 489
            +PI I YYVTW+ P  DSRHLEWTREL+DYM PFVS+SPRAAY+NY+DLDIGRNNEDGI
Sbjct: 429 SHPIHIGYYVTWHQPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGRNNEDGI 488

Query: 490 PTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIPPVA-RGGAQAKMSSCPDV 548
           PTSY+EASVWG +YFG NFE+LVEVKTK+DPFN FRHEQSIPPVA  GG QAKM+SCPDV
Sbjct: 489 PTSYDEASVWGYRYFGINFEKLVEVKTKIDPFNFFRHEQSIPPVASMGGIQAKMASCPDV 548

BLAST of Cla97C03G053540 vs. TrEMBL
Match: tr|A0A1S3CIZ3|A0A1S3CIZ3_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103501494 PE=3 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 5.8e-209
Identity = 342/505 (67.72%), Postives = 415/505 (82.18%), Query Frame = 0

Query: 29  LHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPSQ 88
           + +F+ C+  HSS  +DSNST I+TPSS SYS VLNF+IRNLRFS P  PKP+AII PS 
Sbjct: 1   MEDFVDCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60

Query: 89  PSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVE-DDNTAWV 148
            SQVQ  +ICCK HG+ IR RSGGHDFEG SYVAN+PF+LIDL NLN++T++ +D TAWV
Sbjct: 61  ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDETAWV 120

Query: 149 QSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQL 208
           QSGA++GELYYK+ +++ + GFP G +  +G+GGF SGGG G+MVRKYGLG DNVVDA +
Sbjct: 121 QSGATVGELYYKIGEKSRTLGFPAGFKATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180

Query: 209 VDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPNST 268
           VD NG +++R SMGEDLFWAIRGGGGGSFGIVLAWKL+LV VP+IVTSF   ++W  N+ 
Sbjct: 181 VDRNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240

Query: 269 KLVHRWQYVAPEIDHRLFIGIMVTGANSG---GGRIMGVFMYSLFLGRAEELISIMDKNF 328
            L++RWQY+AP +D  LFI   VT +NS     GRIM    +SLFLG A EL+S+M+K F
Sbjct: 241 NLIYRWQYIAPWVDQDLFISAWVTASNSSKDESGRIMEASFFSLFLGNATELLSLMEKTF 300

Query: 329 PELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPISET 388
           PELGLKK+DC+E +WVESMA+ ASGFV  K +  LLDRTP+ NGRYKTKSDYAT+PIS+T
Sbjct: 301 PELGLKKKDCLEMSWVESMAFSASGFVYAKSLELLLDRTPVDNGRYKTKSDYATEPISKT 360

Query: 389 ALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQDD 448
           AL+G+WERF  + +ETVQL+LIP+GGKMNE SE++TPSPHRAGYPI I YY+TW  P  D
Sbjct: 361 ALKGMWERFKDEELETVQLILIPFGGKMNEISESETPSPHRAGYPIHIGYYLTWQQPNAD 420

Query: 449 SRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFGDN 508
           SR+LEW REL+DYM PFVSKSPRAAYVNYRDLD+G NN+DG+PT Y+EAS+WG +YFG+N
Sbjct: 421 SRNLEWARELHDYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNN 480

Query: 509 FERLVEVKTKVDPFNLFRHEQSIPP 530
           FERL+EVK KVDPFN FRHEQSIPP
Sbjct: 481 FERLMEVKMKVDPFNFFRHEQSIPP 505

BLAST of Cla97C03G053540 vs. TrEMBL
Match: tr|A0A1S3BE31|A0A1S3BE31_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488607 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 7.4e-172
Identity = 300/529 (56.71%), Postives = 387/529 (73.16%), Query Frame = 0

Query: 9   SSFVFLLLLSFLVWDCSGDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIR 68
           +SF+ + ++    W  S  N  +FLHC+S HS     S S  IYTP++PSYSSVLNF+I 
Sbjct: 6   TSFLHIFIVLSFAWAASAHNHESFLHCLSHHSPN-TSSISKIIYTPTNPSYSSVLNFSIH 65

Query: 69  NLRFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLL 128
           NLRF+ P  PKP  I+ P   SQ+Q ++IC KK G  IR RSGGHD+EGLSYV+++ F++
Sbjct: 66  NLRFTSPKTPKPHIIVTPFLVSQIQASIICAKKTGFQIRTRSGGHDYEGLSYVSDVSFVV 125

Query: 129 IDLANLNAVTVE-DDNTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGG 188
           +DL NL +++V+ ++N AWVQSGA++GELYY++++++ + GFP GV P VGVGG FSGGG
Sbjct: 126 VDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGG 185

Query: 189 YGLMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLV 248
           YGLM+RK+GL  DNV+DA  +D NG L DRKSMGED+FWAIRGGGG SFGIVLAWK+KL+
Sbjct: 186 YGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWKIKLL 245

Query: 249 PVPAIVTSFTAFRSWGPNSTKLVHRWQYVAPEIDHRLFIGIMVTGANS-----GGGRIMG 308
           PVP+IVT FT  R+   N+T L+HRWQYV+ + D +LFI I++TG N+     G  R + 
Sbjct: 246 PVPSIVTVFTITRTLEQNATDLIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIE 305

Query: 309 VFMYSLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDR 368
               SLFLG+ EEL+ IM+K FPELGL KEDC+E +W+ES+ Y A GF   + +N LLDR
Sbjct: 306 AAFNSLFLGKIEELVPIMEKTFPELGLTKEDCIEMSWIESVLYFA-GFSRGQPLNVLLDR 365

Query: 369 TPLKNGR-YKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTP 428
            PL   R +K KSDY  +PI +  LEGIWE F+ +  E   L+L PYGG M++ SE++ P
Sbjct: 366 RPLTPKRFFKAKSDYVNEPIPKAGLEGIWELFDEEEAEAAVLILSPYGGIMDKISESEIP 425

Query: 429 SPHRAGYPIKIAYYVTWNSPQDD--SRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIG 488
            PHRAG   KI + V W+   +D   RH+ W R+LY YMAPFVSK+PRAAY+NYRDLDIG
Sbjct: 426 FPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIG 485

Query: 489 RNNEDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIP 529
            N ++G  TSY EASVWG+KYF  NF+RLV VKTKVDP N F++EQSIP
Sbjct: 486 TNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIP 532

BLAST of Cla97C03G053540 vs. TrEMBL
Match: tr|A0A1S3BDB7|A0A1S3BDB7_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488652 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.2e-166
Identity = 292/525 (55.62%), Postives = 382/525 (72.76%), Query Frame = 0

Query: 12  VFLLLLSFLVWDCSGDNLH-NFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNL 71
           + +++ S  +W  +  + H NFLHC+  HSS+     S  +YTP + SYSSVLNF+IRN 
Sbjct: 15  IIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSK-SYPISKVVYTPINSSYSSVLNFSIRNF 74

Query: 72  RFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLID 131
           RFS P  PKP+ II PS  S +Q A+IC K HG+ IR RSGGHD+EGLSYVA  PF+++D
Sbjct: 75  RFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYRPFIIVD 134

Query: 132 LANLNAVTVE-DDNTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYG 191
           L N+ +++V+  +NTAWV+SGA++GELYY++ +++ +  FP G+ P VGVGG FSGGGYG
Sbjct: 135 LINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYG 194

Query: 192 LMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPV 251
            ++RKYGL  DNV+DA LVDANG   DR+SMGEDLFWAIRGGGGGSFGIV+AWK+KLVPV
Sbjct: 195 FLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPV 254

Query: 252 PAIVTSFTAFRSWGPNSTKLVHRWQYVAPEIDHRLFIGIMVTGANS---GGGRIMGVFMY 311
           PA+VT  +  ++   ++ K++H+WQYVA ++   +F+GI++TG N+   GG +      Y
Sbjct: 255 PALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGIKKPTATFY 314

Query: 312 SLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASG-FVTTKDMNFLLDRTPL 371
           SLFLG+ +EL++ +   FPE GL K+DC+E  W+ES    ASG F T + +  LL+RTP 
Sbjct: 315 SLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQTVESLKPLLNRTPS 374

Query: 372 KNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHR 431
                K KSDY  KPI + A+EGIW+R  A  IE  QLV +PYG +M++ SE++TP  HR
Sbjct: 375 TIDSTKIKSDYIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVPYGARMSQISESETPFSHR 434

Query: 432 AGYPIKIAYYVTWNSP--QDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNE 491
           AGY  KI Y V W     +  +RH+ WTRELY+YM PFVSKSPR AYVNYRDLDIG NN+
Sbjct: 435 AGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAYVNYRDLDIGSNNK 494

Query: 492 DGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIP 529
            G  TSY++AS+WGLKYFG+NF+RLV VK KVDP + FRHEQSIP
Sbjct: 495 YG-KTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIP 537

BLAST of Cla97C03G053540 vs. TrEMBL
Match: tr|A0A1S3BDA8|A0A1S3BDA8_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488637 PE=3 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.0e-166
Identity = 298/526 (56.65%), Postives = 380/526 (72.24%), Query Frame = 0

Query: 10  SFVFLLLLS-FLVWDCSGDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIR 69
           S VF+++ S  L    S     NFL C+S HSS+     S  +YTP + SYSSVL+F+IR
Sbjct: 11  SLVFIVVSSASLCASASPQKYQNFLQCLSEHSSK-SYPISKVVYTPINSSYSSVLDFSIR 70

Query: 70  NLRFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLL 129
           NL FS P  PKP+ II PS  S +Q A+IC K HG+ IR RSGGHDFEGLSYVA  PF++
Sbjct: 71  NLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIV 130

Query: 130 IDLANLNAVTVE-DDNTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGG 189
           +DL NL +V VE ++NTAWV+SGA++GELYY++ +++ +  FP GV P VG+GG FSGGG
Sbjct: 131 VDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSGGG 190

Query: 190 YGLMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLV 249
           YGLM+RK+GL  DNV+DA LVDANG +LD++SMGEDLFWAIRGGGGGSFGIV+AWK+KLV
Sbjct: 191 YGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVAWKIKLV 250

Query: 250 PVPAIVTSFTAFRSWGPNSTKLVHRWQYVAPEIDHRLFIGIMVTGANS---GGGRIMGVF 309
           PVP  VT  +  R+   ++ KL+HRWQYV  ++D  +++GI++TG N+    G       
Sbjct: 251 PVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQAGITNPTAR 310

Query: 310 MYSLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTP 369
            +SLFLGRA+EL++ +   FPELGL K+DCVE++WVES      G    + +  LL+RT 
Sbjct: 311 FFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEPLLNRTL 370

Query: 370 LKNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPH 429
                 K KSDY T+PI   A++ IW+R   + IET QLV +PYGG+M++ SE++TP  H
Sbjct: 371 TTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQISESETPFSH 430

Query: 430 RAGYPIKIAYYVTW--NSPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNN 489
           RA Y  KI Y V W   S +   RH+ W RE+Y+YMAPFVSKSPRAAY NYRDLDIG NN
Sbjct: 431 RARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNYRDLDIGSNN 490

Query: 490 EDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIP 529
           + G  TSY+ ASVWGLKYFG+NF+RLV VKTKVDP++ FRHEQSIP
Sbjct: 491 KYG-KTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIP 534

BLAST of Cla97C03G053540 vs. TrEMBL
Match: tr|A0A1S3BDZ5|A0A1S3BDZ5_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488631 PE=3 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.0e-166
Identity = 298/530 (56.23%), Postives = 383/530 (72.26%), Query Frame = 0

Query: 10  SFVFLLLLSFLVWDCSGDNLH-NFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIR 69
           S + +L+ S   W  +  + H NFL C+S HSS+     S  ++TP + SYSSVL+F+IR
Sbjct: 11  SLILILVSSSSSWVSASPHKHQNFLQCLSEHSSKRYPI-SKVVHTPINSSYSSVLDFSIR 70

Query: 70  NLRFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLL 129
           NL FS P  PKP+ II PS  S +Q A+IC K HG+ IR RSGGHDFEGLSYVA  PF++
Sbjct: 71  NLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYHPFII 130

Query: 130 IDLANLNAVTVE-DDNTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGG 189
           +DL NL ++ ++ + NTAWV+SGA++GELYY++ +++ +  FPGGV P VGVGG FSGGG
Sbjct: 131 VDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTVGVGGHFSGGG 190

Query: 190 YGLMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLV 249
           YGLM+RK+GL  DNV+DA LVDANG + DRKSMGEDLFWAIRGGGGGSFGIV+AWK+KLV
Sbjct: 191 YGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGIVVAWKIKLV 250

Query: 250 PVPAIVTSFTAFRSWGPNSTKLVHRWQYVAPEIDHRLFIGIMVTGAN-----SGGGRIMG 309
            VPA VT  +  R+   +  KLVH+WQYVA ++D  LF+GIM+TG N      G    + 
Sbjct: 251 SVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITTTQEGIANPIA 310

Query: 310 VFMYSLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDR 369
            F +SLFLG+ + L++ +   FPELG  KEDCVE++W+ES    A+G  T + +  LL+R
Sbjct: 311 TF-FSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTVESLEPLLNR 370

Query: 370 TP--LKNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDT 429
           TP  L+NG  K KSDY  +PIS   +EG+W+R  AK IET QL+ IPYGG+M++ SE +T
Sbjct: 371 TPSNLENG--KMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMSQISEMET 430

Query: 430 PSPHRAGYPIKIAYYVTW--NSPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDI 489
           P  HRAG   KI Y+V W   S +   +H+ W R++Y YM PFVSKSPR+AY NYRDLDI
Sbjct: 431 PFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYANYRDLDI 490

Query: 490 GRNNEDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIP 529
           G N + G  TS  +AS+WGLKYFG+NF+RLV VKTKVDP++ FRHEQSIP
Sbjct: 491 GANKKYG-KTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIP 535

BLAST of Cla97C03G053540 vs. Swiss-Prot
Match: sp|Q9FKU8|BBE26_ARATH (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 2.0e-135
Identity = 242/506 (47.83%), Postives = 339/506 (67.00%), Query Frame = 0

Query: 32  FLHCVSPHSSQLDDSNSTFIY-TPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPSQPS 91
           F++CV  ++        TF   T +   +S VL  T +NLRF   ++PKP  I  P   S
Sbjct: 35  FINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHES 94

Query: 92  QVQTALICCKKHGIHIRIRSGGHDFEGLSYVANL--PFLLIDLANLNAVTVE-DDNTAWV 151
            VQ ++IC KK  +H+R+RSGGHD+EGLSYV+ +  PF+L+DL+ +  V +   DN+AWV
Sbjct: 95  HVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWV 154

Query: 152 QSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQL 211
           QSGA++GELYY++++++   GFP G+  ++G+GG  +GG YG M+RKYGLG DNV+DA++
Sbjct: 155 QSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKI 214

Query: 212 VDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPN-S 271
           VDANG LLDR +MGED FWAIRGG GGSFGI+LAWK+KLVPVP  VT FT  ++   +  
Sbjct: 215 VDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVG 274

Query: 272 TKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNFPE 331
            K++ +WQ VA ++   LFI ++   A +GG + +     +LFLG    L+++M K+FPE
Sbjct: 275 NKIISKWQRVADKLVEELFIRVLFNVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPE 334

Query: 332 LGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLD-RTPLKNGRYKTKSDYATKPISETA 391
           LGL  +DC+E +W+ES+AY  SGF T    N LL  ++P     +K KSD+   PI E+ 
Sbjct: 335 LGLTFKDCIEMSWLESIAY-ISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESG 394

Query: 392 LEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTW-NSPQDD 451
           L+GI+++   + I    ++  PYGG M +  E+  P PHR G   K+ Y  +W +S +  
Sbjct: 395 LQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRP 454

Query: 452 SRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFGDN 511
           SRH+ W R+LY YM P+VS +PR AYVNYRDLD+GRN +D + T  ++A VWG  YF +N
Sbjct: 455 SRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKD-VKTCIKQAQVWGANYFKNN 514

Query: 512 FERLVEVKTKVDPFNLFRHEQSIPPV 531
           F RL+ +K KVDP N FRHEQSIPP+
Sbjct: 515 FNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Cla97C03G053540 vs. Swiss-Prot
Match: sp|Q33DQ2|THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.6e-135
Identity = 241/530 (45.47%), Postives = 339/530 (63.96%), Query Frame = 0

Query: 12  VFLLLLSFLVWDCSGDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLR 71
           +    LSF +     +   NFL C S +    + +N  FIYT     Y SVLN TI+NLR
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKCFSEYIPN-NPANPKFIYTQHDQLYMSVLNSTIQNLR 73

Query: 72  FSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDL 131
           F+    PKP+ I+ PS  S +Q +++C KK G+ IR RSGGHD EGLSY++ +PF ++DL
Sbjct: 74  FTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDL 133

Query: 132 ANLNAVTVE-DDNTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGL 191
            N++ V V+    TAWV++GA++GE+YY +++   +F FPGG  P VGVGG FSGGGYG 
Sbjct: 134 RNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGA 193

Query: 192 MVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVP 251
           ++R YGL  DN++DA LV+ +G +LDRKSMGEDLFWAIRGGGG +FGI+ AWK+KLV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVP 253

Query: 252 AIVTSFTAFRSWGPNS-TKLVHRWQYVAPEIDHRLFIGI-----MVTGANSGGGRIMGVF 311
           +  T F+  ++   +   KL ++WQ +A + D  L +        +T  +      +  +
Sbjct: 254 SKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGY 313

Query: 312 MYSLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNF----LL 371
             S+FLG  + L+ +M+K+FPELG+KK DC E +W+++  +  SG V     NF    LL
Sbjct: 314 FSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIF-YSGVVNYNTANFKKEILL 373

Query: 372 DRTPLKNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDT 431
           DR+  K   +  K DY  K I ETA+  I E+   + +     VL PYGG M+E SE+  
Sbjct: 374 DRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAI 433

Query: 432 PSPHRAGYPIKIAYYVTWNSPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGR 491
           P PHRAG   ++ Y  TW   +D+ +H+ W R +Y++  P+VS++PR AY+NYRDLD+G+
Sbjct: 434 PFPHRAGIMYELWYTATWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGK 493

Query: 492 NNEDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIPPV 531
            N +  P +Y +A +WG KYFG NF RLV+VKTK DP N FR+EQSIPP+
Sbjct: 494 TNPES-PNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of Cla97C03G053540 vs. Swiss-Prot
Match: sp|Q8GTB6|THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 PE=1 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 2.8e-134
Identity = 237/530 (44.72%), Postives = 338/530 (63.77%), Query Frame = 0

Query: 12  VFLLLLSFLVWDCSGDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLR 71
           +    LSF +     +   NFL C S H    + +N   +YT     Y S+LN TI+NLR
Sbjct: 14  IIFFFLSFHIQISIANPRENFLKCFSKHIPN-NVANPKLVYTQHDQLYMSILNSTIQNLR 73

Query: 72  FSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDL 131
           F     PKP+ I+ PS  S +Q  ++C KK G+ IR RSGGHD EG+SY++ +PF+++DL
Sbjct: 74  FISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDL 133

Query: 132 ANLNAVTVE-DDNTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGL 191
            N++++ ++    TAWV++GA++GE+YY ++++  +  FPGG  P VGVGG FSGGGYG 
Sbjct: 134 RNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGA 193

Query: 192 MVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVP 251
           ++R YGL  DN++DA LV+ +G +LDRKSMGEDLFWAIRGGGG +FGI+ AWK+KLV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVP 253

Query: 252 AIVTSFTAFRSWGPNS-TKLVHRWQYVAPEIDHRL-----FIGIMVTGANSGGGRIMGVF 311
           +  T F+  ++   +   KL ++WQ +A + D  L     FI   +T  +      +  +
Sbjct: 254 SKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGY 313

Query: 312 MYSLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNF----LL 371
             S+F G  + L+ +M+K+FPELG+KK DC E +W+++  +  SG V     NF    LL
Sbjct: 314 FSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIF-YSGVVNFNTANFKKEILL 373

Query: 372 DRTPLKNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDT 431
           DR+  K   +  K DY  KPI ETA+  I E+   + +     VL PYGG M E SE+  
Sbjct: 374 DRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAI 433

Query: 432 PSPHRAGYPIKIAYYVTWNSPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGR 491
           P PHRAG   ++ Y  +W   +D+ +H+ W R +Y++  P+VS++PR AY+NYRDLD+G+
Sbjct: 434 PFPHRAGIMYELWYTASWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGK 493

Query: 492 NNEDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIPPV 531
            N    P +Y +A +WG KYFG NF RLV+VKTKVDP N FR+EQSIPP+
Sbjct: 494 TNH-ASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Cla97C03G053540 vs. Swiss-Prot
Match: sp|Q9FI25|BBE27_ARATH (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.4e-133
Identity = 236/532 (44.36%), Postives = 342/532 (64.29%), Query Frame = 0

Query: 9   SSFVFLLLLS-----FLVWDCSGDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVL 68
           S F++ LLL+     F        N  NFL C+   S ++++ +S  I+T   PSY S+L
Sbjct: 9   SLFIYFLLLNLSLSHFPSISAQRTNHENFLKCL---SHRINEDDSRIIHTSKDPSYFSIL 68

Query: 69  NFTIRNLRFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVA- 128
           N +I+N RF     PKP++II P Q + VQ+ + C + HGIHIR RSGGHD+EGLSY+A 
Sbjct: 69  NSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAK 128

Query: 129 NLPFLLIDLANLNAVTVEDDN-TAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGG 188
           + PF++IDL NL ++T++ DN T WVQSGA++GELYY++ + + S  FP G+ P VG+GG
Sbjct: 129 SRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGG 188

Query: 189 FFSGGGYGLMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLA 248
            F GGGYG ++RKYGL  DNV+DA +VDANG  LDR+ MGED FWAIRGGGG SF +VL+
Sbjct: 189 QFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLS 248

Query: 249 WKLKLVPVPAIVTSFTAFRSWGPNSTKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIM 308
           WK++L+ VP++VT F   ++    +  ++++WQY+A ++ + LFI  M+           
Sbjct: 249 WKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKETEVYASFP 308

Query: 309 GVFMYSLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLD 368
           G     L+LG   +L+++M   FPELGL+  +C E +W+ES+ +    F+  + M  L  
Sbjct: 309 G-----LYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLW----FIKGESMEILAK 368

Query: 369 RTPLKNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTP 428
           R       +K K D+  +PI +TA++ +W RF A      +++L P+GGKM+E ++ + P
Sbjct: 369 RKRTSRS-FKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIP 428

Query: 429 SPHRAGYPIKIAYYVTWNSPQDDS-----RHLEWTRELYDYMAPFVSKSPRAAYVNYRDL 488
            PHR G   +I Y   W+  +D +     ++L W   +Y++M P+VSKSPR AYVN+RD+
Sbjct: 429 FPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDI 488

Query: 489 DIGRNNEDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIP 529
           D+G      + T YEEA VWG+KYF +NF+RLV VKT VDP + F  EQSIP
Sbjct: 489 DLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of Cla97C03G053540 vs. Swiss-Prot
Match: sp|Q9SVG5|BBE18_ARATH (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 3.1e-133
Identity = 237/506 (46.84%), Postives = 341/506 (67.39%), Query Frame = 0

Query: 28  NLHNFLHCVSPHSSQLDDSN--STFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIK 87
           N  +FL C+   S QL+DSN  S  I+TP+  S+SSVL  +I+N RFS P++PKP+ I+ 
Sbjct: 33  NQSSFLQCL---SLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILT 92

Query: 88  PSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVEDDN-T 147
           P QPS VQ+A+ C ++ GIHIR RSGGHD+EGLSYV + PF+++DL NL ++TV+ DN +
Sbjct: 93  PVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRS 152

Query: 148 AWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVD 207
            WVQ+GA++GELYY++ ++  +  FP GV P VGVGG FSGGGYG ++RK+GL  D+V+D
Sbjct: 153 VWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVID 212

Query: 208 AQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGP 267
           A++VDA G +L+R+ MGED FWAIRGGGG SF +VL+WK+ L+ VP+ VT F   +    
Sbjct: 213 ARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQ 272

Query: 268 NSTKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNF 327
           ++ K++HRWQ+VA ++   LFI +M+    +    ++      L+LG  + L+ +++K F
Sbjct: 273 SALKIIHRWQFVADKVSDDLFIRVMLQRYKN----MVRASFPGLYLGSVKNLLKMVNKEF 332

Query: 328 PELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPISET 387
           PELGL+++DC E +W+ES+ + A   +  + +N L  RT      +K KSD+  +P+ +T
Sbjct: 333 PELGLEEDDCTEMSWIESVIWFAE--LGEEPINVLTKRTRASLA-FKAKSDFVQEPMPKT 392

Query: 388 ALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQDD 447
           A+  +W R      E  QL+  P+GGKM+E ++ +TP PHR G   +I Y   W     +
Sbjct: 393 AISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRGDVKE 452

Query: 448 SRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTS-YEEASVWGLKYFGD 507
            +++ W   +YD M+ FV+KSPR AY+N RDLD+G     G+  S YEE   WG+KYF +
Sbjct: 453 -KYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM--YVGVKRSKYEEGKSWGVKYFKN 512

Query: 508 NFERLVEVKTKVDPFNLFRHEQSIPP 530
           NFERLV VKT VDP + F  EQSIPP
Sbjct: 513 NFERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of Cla97C03G053540 vs. TAIR10
Match: AT5G44400.1 (FAD-binding Berberine family protein)

HSP 1 Score: 484.2 bits (1245), Expect = 1.1e-136
Identity = 242/506 (47.83%), Postives = 339/506 (67.00%), Query Frame = 0

Query: 32  FLHCVSPHSSQLDDSNSTFIY-TPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPSQPS 91
           F++CV  ++        TF   T +   +S VL  T +NLRF   ++PKP  I  P   S
Sbjct: 35  FINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHES 94

Query: 92  QVQTALICCKKHGIHIRIRSGGHDFEGLSYVANL--PFLLIDLANLNAVTVE-DDNTAWV 151
            VQ ++IC KK  +H+R+RSGGHD+EGLSYV+ +  PF+L+DL+ +  V +   DN+AWV
Sbjct: 95  HVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWV 154

Query: 152 QSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQL 211
           QSGA++GELYY++++++   GFP G+  ++G+GG  +GG YG M+RKYGLG DNV+DA++
Sbjct: 155 QSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKI 214

Query: 212 VDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPN-S 271
           VDANG LLDR +MGED FWAIRGG GGSFGI+LAWK+KLVPVP  VT FT  ++   +  
Sbjct: 215 VDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVG 274

Query: 272 TKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNFPE 331
            K++ +WQ VA ++   LFI ++   A +GG + +     +LFLG    L+++M K+FPE
Sbjct: 275 NKIISKWQRVADKLVEELFIRVLFNVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPE 334

Query: 332 LGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLD-RTPLKNGRYKTKSDYATKPISETA 391
           LGL  +DC+E +W+ES+AY  SGF T    N LL  ++P     +K KSD+   PI E+ 
Sbjct: 335 LGLTFKDCIEMSWLESIAY-ISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESG 394

Query: 392 LEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTW-NSPQDD 451
           L+GI+++   + I    ++  PYGG M +  E+  P PHR G   K+ Y  +W +S +  
Sbjct: 395 LQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRP 454

Query: 452 SRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFGDN 511
           SRH+ W R+LY YM P+VS +PR AYVNYRDLD+GRN +D + T  ++A VWG  YF +N
Sbjct: 455 SRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKD-VKTCIKQAQVWGANYFKNN 514

Query: 512 FERLVEVKTKVDPFNLFRHEQSIPPV 531
           F RL+ +K KVDP N FRHEQSIPP+
Sbjct: 515 FNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Cla97C03G053540 vs. TAIR10
Match: AT5G44410.1 (FAD-binding Berberine family protein)

HSP 1 Score: 478.0 bits (1229), Expect = 7.8e-135
Identity = 236/532 (44.36%), Postives = 342/532 (64.29%), Query Frame = 0

Query: 9   SSFVFLLLLS-----FLVWDCSGDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVL 68
           S F++ LLL+     F        N  NFL C+   S ++++ +S  I+T   PSY S+L
Sbjct: 9   SLFIYFLLLNLSLSHFPSISAQRTNHENFLKCL---SHRINEDDSRIIHTSKDPSYFSIL 68

Query: 69  NFTIRNLRFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVA- 128
           N +I+N RF     PKP++II P Q + VQ+ + C + HGIHIR RSGGHD+EGLSY+A 
Sbjct: 69  NSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAK 128

Query: 129 NLPFLLIDLANLNAVTVEDDN-TAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGG 188
           + PF++IDL NL ++T++ DN T WVQSGA++GELYY++ + + S  FP G+ P VG+GG
Sbjct: 129 SRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGG 188

Query: 189 FFSGGGYGLMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLA 248
            F GGGYG ++RKYGL  DNV+DA +VDANG  LDR+ MGED FWAIRGGGG SF +VL+
Sbjct: 189 QFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLS 248

Query: 249 WKLKLVPVPAIVTSFTAFRSWGPNSTKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIM 308
           WK++L+ VP++VT F   ++    +  ++++WQY+A ++ + LFI  M+           
Sbjct: 249 WKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKETEVYASFP 308

Query: 309 GVFMYSLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLD 368
           G     L+LG   +L+++M   FPELGL+  +C E +W+ES+ +    F+  + M  L  
Sbjct: 309 G-----LYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLW----FIKGESMEILAK 368

Query: 369 RTPLKNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTP 428
           R       +K K D+  +PI +TA++ +W RF A      +++L P+GGKM+E ++ + P
Sbjct: 369 RKRTSRS-FKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIP 428

Query: 429 SPHRAGYPIKIAYYVTWNSPQDDS-----RHLEWTRELYDYMAPFVSKSPRAAYVNYRDL 488
            PHR G   +I Y   W+  +D +     ++L W   +Y++M P+VSKSPR AYVN+RD+
Sbjct: 429 FPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDI 488

Query: 489 DIGRNNEDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIP 529
           D+G      + T YEEA VWG+KYF +NF+RLV VKT VDP + F  EQSIP
Sbjct: 489 DLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of Cla97C03G053540 vs. TAIR10
Match: AT4G20820.1 (FAD-binding Berberine family protein)

HSP 1 Score: 476.9 bits (1226), Expect = 1.7e-134
Identity = 237/506 (46.84%), Postives = 341/506 (67.39%), Query Frame = 0

Query: 28  NLHNFLHCVSPHSSQLDDSN--STFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIK 87
           N  +FL C+   S QL+DSN  S  I+TP+  S+SSVL  +I+N RFS P++PKP+ I+ 
Sbjct: 33  NQSSFLQCL---SLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILT 92

Query: 88  PSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVEDDN-T 147
           P QPS VQ+A+ C ++ GIHIR RSGGHD+EGLSYV + PF+++DL NL ++TV+ DN +
Sbjct: 93  PVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRS 152

Query: 148 AWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVD 207
            WVQ+GA++GELYY++ ++  +  FP GV P VGVGG FSGGGYG ++RK+GL  D+V+D
Sbjct: 153 VWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVID 212

Query: 208 AQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGP 267
           A++VDA G +L+R+ MGED FWAIRGGGG SF +VL+WK+ L+ VP+ VT F   +    
Sbjct: 213 ARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQ 272

Query: 268 NSTKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNF 327
           ++ K++HRWQ+VA ++   LFI +M+    +    ++      L+LG  + L+ +++K F
Sbjct: 273 SALKIIHRWQFVADKVSDDLFIRVMLQRYKN----MVRASFPGLYLGSVKNLLKMVNKEF 332

Query: 328 PELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPISET 387
           PELGL+++DC E +W+ES+ + A   +  + +N L  RT      +K KSD+  +P+ +T
Sbjct: 333 PELGLEEDDCTEMSWIESVIWFAE--LGEEPINVLTKRTRASLA-FKAKSDFVQEPMPKT 392

Query: 388 ALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQDD 447
           A+  +W R      E  QL+  P+GGKM+E ++ +TP PHR G   +I Y   W     +
Sbjct: 393 AISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRGDVKE 452

Query: 448 SRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTS-YEEASVWGLKYFGD 507
            +++ W   +YD M+ FV+KSPR AY+N RDLD+G     G+  S YEE   WG+KYF +
Sbjct: 453 -KYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM--YVGVKRSKYEEGKSWGVKYFKN 512

Query: 508 NFERLVEVKTKVDPFNLFRHEQSIPP 530
           NFERLV VKT VDP + F  EQSIPP
Sbjct: 513 NFERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of Cla97C03G053540 vs. TAIR10
Match: AT5G44380.1 (FAD-binding Berberine family protein)

HSP 1 Score: 466.5 bits (1199), Expect = 2.3e-131
Identity = 235/507 (46.35%), Postives = 338/507 (66.67%), Query Frame = 0

Query: 32  FLHCVSPHSSQLDDSNSTFIYTPSS--PSYSSVLNFTIRNLRFSGPNIPKPIAIIKPSQP 91
           F++CV  ++        T ++TP+     ++ VL  T +NL+F   ++PKP  I +P   
Sbjct: 38  FINCVKRNTHVSFPLEKT-LFTPAKNVSLFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQ 97

Query: 92  SQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANL--PFLLIDLANLNAVTVE-DDNTAW 151
           SQVQ ++IC KK GIH R+RSGGHDFE LSYV+ +  PF+L+DL+ L  + V+ + N+AW
Sbjct: 98  SQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLSKLKQINVDIESNSAW 157

Query: 152 VQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQ 211
           VQ GA++GELYY++++++   GFP G+  +VG+GG+ +GGGYG ++RKYGL  DNV+D +
Sbjct: 158 VQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVK 217

Query: 212 LVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPNS 271
           +VDANG LLDR +MGEDLFWAIRGGGG SFGIVLAWK+KLVPVP  VT FT  ++   ++
Sbjct: 218 MVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDA 277

Query: 272 -TKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNFP 331
             K + +WQ ++ +I   + I +++  A + G + + +     FLG    L+ +M+K FP
Sbjct: 278 RLKTISKWQQISSKIIEEIHIRVVLRAAGNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFP 337

Query: 332 ELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLD-RTPLKNGRYKTKSDYATKPISET 391
           ELGL ++DC E +W+E+  +   GF T   +  LL  ++PL    +K  SD+  +PI   
Sbjct: 338 ELGLTQKDCTEMSWIEAALF-HGGFPTGSPIEILLQLKSPLGKDYFKATSDFVKEPIPVI 397

Query: 392 ALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTW-NSPQD 451
            L+GI++R   +G  T  L   PYGG M++  E+  P PHR G   KI YY  W  + + 
Sbjct: 398 GLKGIFKRL-IEG-NTTFLNWTPYGGMMSKIPESAIPFPHRNGTLFKILYYANWLENDKT 457

Query: 452 DSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFGD 511
            SR + W +E+Y+YMAP+VS +PR AYVNYRDLD G+ N++    ++ EA +WG KYF  
Sbjct: 458 SSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQ-NKNNAKVNFIEAKIWGPKYFKG 517

Query: 512 NFERLVEVKTKVDPFNLFRHEQSIPPV 531
           NF+RLV++KTKVDP N FRHEQSIPP+
Sbjct: 518 NFDRLVKIKTKVDPENFFRHEQSIPPM 539

BLAST of Cla97C03G053540 vs. TAIR10
Match: AT1G30760.1 (FAD-binding Berberine family protein)

HSP 1 Score: 465.7 bits (1197), Expect = 4.0e-131
Identity = 238/534 (44.57%), Postives = 349/534 (65.36%), Query Frame = 0

Query: 5   MGNYSSF-VFLLLLSFLVWDCSGDNL-HNFLHCVSPHS-SQLDDSNSTFIYTPSSPSYSS 64
           M N ++F V LLLLS      S   +  +F+ C+  +S +     +S F +  ++ S+  
Sbjct: 6   MNNTNAFLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKL 65

Query: 65  VLNFTIRNLRFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYV 124
            L  + +NLR+  P+ PKP  I +P   + VQ A++C KK  +H+R+RSGGHD+EGLSYV
Sbjct: 66  ALETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYV 125

Query: 125 ANL--PFLLIDLANLNAVTVE-DDNTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVG 184
           + +   F+++DL+ L  ++V+ + N+AWV +GAS+GE+YY++ +++   GFP G+  ++G
Sbjct: 126 SEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLG 185

Query: 185 VGGFFSGGGYGLMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGI 244
           +GG   GG YG M+RK+GLG DNV+DA++VDA+G +L+R +MGED+FWAIRGGGGGSFG+
Sbjct: 186 IGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGV 245

Query: 245 VLAWKLKLVPVPAIVTSFTAFRSWGPNSTKLVHRWQYVAPEIDHRLFIGIMVTGAN---S 304
           +LAWK+KLVPVP IVT FT  R+   + TKL+++WQ VA ++D  LFI +++   +    
Sbjct: 246 ILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPK 305

Query: 305 GGGRIMGVFMYSLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKD 364
              R +       FLG A  L+ +M ++FP+LGL K+DC+E++W++S+ Y A GF +T  
Sbjct: 306 SKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIA-GFPSTAP 365

Query: 365 MNFLLDRTPLKNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEF 424
              LLD   L    +K KSDY  +PI    LEG+WE+   +  ++   +  PYGG M + 
Sbjct: 366 SEALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEE--DSPLTIWNPYGGMMAKI 425

Query: 425 SETDTPSPHRAGYPIKIAYYVTW-NSPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYR 484
            ET+TP PHR+G   KI +   W +    +++H+ W RE+Y YM  +VSKSPR+AYVNYR
Sbjct: 426 PETETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYR 485

Query: 485 DLDIGRNNEDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIP 529
           DLD+G N +        +A  WG +YF  NFERLVE+K K DP N FRHEQSIP
Sbjct: 486 DLDLGMNGKG------SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022964765.15.2e-25479.67berberine bridge enzyme-like 26 [Cucurbita moschata][more]
XP_023521064.12.2e-25281.36berberine bridge enzyme-like 26 [Cucurbita pepo subsp. pepo][more]
XP_023525672.12.5e-23273.78berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo][more]
XP_022964764.12.1e-22368.74berberine bridge enzyme-like 18 [Cucurbita moschata][more]
XP_023521065.13.1e-22269.19berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
tr|A0A1S3CIZ3|A0A1S3CIZ3_CUCME5.8e-20967.72tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10350149... [more]
tr|A0A1S3BE31|A0A1S3BE31_CUCME7.4e-17256.71tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348860... [more]
tr|A0A1S3BDB7|A0A1S3BDB7_CUCME1.2e-16655.62tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348865... [more]
tr|A0A1S3BDA8|A0A1S3BDA8_CUCME6.0e-16656.65tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348863... [more]
tr|A0A1S3BDZ5|A0A1S3BDZ5_CUCME6.0e-16656.23tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348863... [more]
Match NameE-valueIdentityDescription
sp|Q9FKU8|BBE26_ARATH2.0e-13547.83Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=... [more]
sp|Q33DQ2|THCAI_CANSA2.6e-13545.47Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 PE=3 SV... [more]
sp|Q8GTB6|THCAS_CANSA2.8e-13444.72Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 PE=1 SV=1[more]
sp|Q9FI25|BBE27_ARATH1.4e-13344.36Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=... [more]
sp|Q9SVG5|BBE18_ARATH3.1e-13346.84Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=... [more]
Match NameE-valueIdentityDescription
AT5G44400.11.1e-13647.83FAD-binding Berberine family protein[more]
AT5G44410.17.8e-13544.36FAD-binding Berberine family protein[more]
AT4G20820.11.7e-13446.84FAD-binding Berberine family protein[more]
AT5G44380.12.3e-13146.35FAD-binding Berberine family protein[more]
AT1G30760.14.0e-13144.57FAD-binding Berberine family protein[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0071949FAD binding
GO:0003824catalytic activity
GO:0050660flavin adenine dinucleotide binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR036318FAD-bd_2-like_sf
IPR016166FAD-bd_2
IPR016167FAD-bd_2_sub1
IPR006094Oxid_FAD_bind_N
IPR012951BBE
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006040 amino sugar metabolic process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
molecular_function GO:0008762 UDP-N-acetylmuramate dehydrogenase activity
molecular_function GO:0071949 FAD binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C03G053540.1Cla97C03G053540.1mRNA


Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.465.50coord: 466..529
e-value: 2.1E-147
score: 493.4
coord: 141..251
e-value: 2.1E-147
score: 493.4
coord: 338..365
e-value: 2.1E-147
score: 493.4
NoneNo IPR availableGENE3DG3DSA:3.40.462.20coord: 366..465
e-value: 2.1E-147
score: 493.4
coord: 252..337
e-value: 2.1E-147
score: 493.4
NoneNo IPR availablePANTHERPTHR32448FAMILY NOT NAMEDcoord: 7..530
NoneNo IPR availablePANTHERPTHR32448:SF53SUBFAMILY NOT NAMEDcoord: 7..530
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 469..528
e-value: 8.5E-22
score: 76.9
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 80..215
e-value: 1.7E-28
score: 99.0
IPR016167FAD-binding, type 2, subdomain 1GENE3DG3DSA:3.30.43.10coord: 27..134
e-value: 4.0E-38
score: 131.5
IPR016166FAD-binding, type 2PROSITEPS51387FAD_PCMHcoord: 76..249
score: 19.191
IPR036318FAD-binding, type 2-like superfamilySUPERFAMILYSSF56176FAD-binding/transporter-associated domain-likecoord: 48..250

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cla97C03G053540Silver-seed gourdcarwmbB0467
Cla97C03G053540Cucumber (Gy14) v2cgybwmbB034
Cla97C03G053540Cucumber (Gy14) v1cgywmbB386
Cla97C03G053540Cucurbita moschata (Rifu)cmowmbB209
Cla97C03G053540Wild cucumber (PI 183967)cpiwmbB034
Cla97C03G053540Cucumber (Chinese Long) v3cucwmbB032
Cla97C03G053540Cucumber (Chinese Long) v2cuwmbB034
Cla97C03G053540Melon (DHL92) v3.6.1medwmbB142
Cla97C03G053540Melon (DHL92) v3.5.1mewmbB153
Cla97C03G053540Wax gourdwgowmbB481